14-3-3 zeta/beta Antibody (22-IID8B)

Western Blot: 14-3-3 zeta/beta Antibody (22-IID8B) [NB100-1964] - Human epidermoid carcinoma A431 cell lysate.

Product Discontinued

14-3-3 zeta/beta Antibody (22-IID8B) Summary

• Immunogen: Lysed transformed amnion cells (AMA) (Human).
• Localization: Cytoplasmic
• Isotype: IgG
• Clonality: Monoclonal
• Host: Mouse
• Gene: YWHAZ
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1 ug/ml
• Theoretical MW: 28 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  14-3-3 zeta Overexpression Lysate
  HeLa Whole Cell Lysate

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2), 0.1mM PMSF and 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for 14-3-3 zeta/beta Antibody (22-IID8B)

• KCIP-1MGC126532,14-3-3-zeta
• MGC111427
• MGC138156
• tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, deltapolypeptide
• tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zetapolypeptide
• zeta polypeptide

Background

14-3-3 proteins are highly conserved proteins which play a role in both signal transduction and progression through the cell cycle by binding to and regulating several different proteins. 14-3-3 proteins activate tyrosine and tryptophan hydroxylases and protein kinase C. They mediate signal transduction by binding to phosphoserine-containing proteins. There are at least 7 mammalian isoforms: alpha, beta, gamma, delta, epsilon, zeta, and eta. An eighth subtype, termed theta has been found in rat brain. The 14-3-3 proteins exist in vitro and in vivo as either homo- or heterodimers which interact via their N-terminal domains and are subject to phosphorylation by protein kinase C. 14-3-3 proteins are localized in the cytoplasm of neurons in the cerebral cortex and are axonally transported to the nerve terminals. They may be present at lower levels in various other eukaryotic tissues. Northern blot analysis has shown expression of the eta chain in cultured cell lines derived from various tumors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 14-3-3 zeta/beta Antibody (NB100-1964)(3)

Publications using NB100-1964

• Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018-01-01
• Yu DS, Weng TH, Hu CY et al. Chaperones, Membrane Trafficking and Signal Transduction Proteins Regulate Zaire Ebola Virus trVLPs and Interact With trVLP Elements. Front Microbiol. 2018-11-12 [PMID: 30483236] (WB, Human)
• Choi EK, Zaidi NF, Miller JS et al. Calsenilin is a substrate for caspase-3 that preferentially interacts with the familial Alzheimer's disease-associated C-terminal fragment of presenilin 2. J Biol Chem. 2001-06-01 [PMID: 11278424]

Bioinformatics

• Gene Symbol: YWHAZ
• Entrez:
  • 7534(Human)
• Uniprot:
  • P63104()