14-3-3 zeta Antibody

Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, Raji human Burkitt's lymphoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheo-chromocytoma cell line. PVDF membrane was probed with 0.25 µg/mL Goat Anti-Human/ Mouse 14-3-3 zeta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2669) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human 14-3-3 beta, epsilon, eta, gamma, sigma, and zeta (2 ng/lane) were included. A specific band for 14-3-3 zeta was detected at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

14-3-3 zeta Antibody Summary

• Immunogen: E. coli-derived recombinant human 14-3-3 zeta
  Met1-Asn245
  Accession # P63104
• Specificity: Detects human, mouse, and rat 14-3-3 zeta in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human 14‑3‑3 beta, epsilon, eta, gamma, or sigma is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: YWHAZ
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for 14-3-3 zeta Antibody

• 1433 zeta
• 14-3-3 zeta
• KCIP-1
• KCIP-1MGC126532,14-3-3-zeta
• MGC111427
• MGC138156
• tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, deltapolypeptide
• tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zetapolypeptide
• YWHAZ
• zeta polypeptide

Background

14-3-3 proteins are a highly conserved family of homo- and heterodimeric phosphoserine/threonine-binding proteins present in high abundance in all eukaryotic cells. 14-3-3 proteins were the first polypeptides shown to have pSer/Thr binding properties, generally recognizing the consensus sequences RSXpSXP and RXY/FXpSXP (where X is any amino acid). 14-3-3 proteins act as key regulators of intracellular signal transduction through their ability to bind specific motifs phosphorylated on serine or threonine. For example, the binding of 14-3-3 to phosphorylated BAD blocks its proapoptotic association with Bcl-XL. There are at least seven distinct 14-3-3 genes in vertebrates, alpha/beta, epsilon, eta, gamma, theta, sigma and zeta ( alpha / beta , epsilon , eta , gamma , tau / theta , sigma , and zeta /δ). 14-3-3 zeta, also known as Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein, zeta isoform (YWHAZ) is a 245 amino acid, 27 kDa protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 14-3-3 zeta Antibody (AF2669)(2)

Publications using AF2669

• Toyo-oka K, Wachi T, Hunt R, Baraban S, Taya S, Ramshaw H, Kaibuchi K, Schwarz Q, Lopez A, Wynshaw-Boris A 14-3-3epsilon and zeta regulate neurogenesis and differentiation of neuronal progenitor cells in the developing brain. J Neurosci, 2014-09-03;34(36):12168-81. 2014-09-03 [PMID: 25186760] (Western Blot, Mouse)
• Yu KH, Barry CG, Austin D Stable isotope dilution multidimensional liquid chromatography-tandem mass spectrometry for pancreatic cancer serum biomarker discovery. J. Proteome Res., 2009-03-01;8(3):1565-76. 2009-03-01 [PMID: 19199705] (Western Blot, Human)

Review for 14-3-3 zeta Antibody (AF2669) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer Simple Western Mouse 07/29/2019

Summary

• Application: Simple Western
• Sample Tested: Mouse kidney,Mouse Spleen
• Species: Mouse
• Lot: UVJ0208091

Bioinformatics

• Gene Symbol: YWHAZ
• Uniprot:
  • P63104()