14-3-3 Antibody (JJ084-3)

Western Blot: 14-3-3 Antibody (JJ084-3) [NBP2-67866] - Analysis of 14-3-3 on different lysates using anti-14-3-3 antibody at 1/1,000 dilution. Positive control: Lane 1: HT29 Lane 2: Human skin Lane 3: NIH/3T3

Western Blot: 14-3-3 Antibody (JJ084-3) [NBP2-67866] - Western blot analysis of 14-3-3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Ra

Product Discontinued

14-3-3 Antibody (JJ084-3) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human 14-3-3 aa 1-122 / 246. (SwissProt: P31946 Human; SwissProt: P63104 Human; SwissProt: P63101 Mouse; SwissProt: Q9CQV8 Mouse; SwissProt: P35213 Rat; SwissProt: P63102 Rat)
• Localization: Cytoplasm, Melanosome.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: YWHAQ
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for 14-3-3 Antibody (JJ084-3)

• 14-3-3 protein tau
• 14-3-3 protein T-cell
• 14-3-3 protein theta
• 1433
• 14-3-3
• 14-3-3,1C5
• HS1
• Protein HS1
• protein tau
• tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, thetapolypeptide

Background

14-3-3 family of proteins is composed of seven isotypes, beta, gamma, zeta, epsilon, tau, eta, and sigma, that play critical roles in cell signaling events that control cell cycle progression, transcriptional alterations, and apoptosis (1-4). 14-3-3 proteins were the first signaling molecules to be identified as specific phosphoserine/threonine binding proteins (1). 14-3-3 can serve as a direct regulator of its target by altering the function of the protein (3). 14-3-3 protein targets include the signalling intermediates Raf, MEKK, PI-3 kinase and IRS-1, cell cycle proteins CDK2, Wee1, and Cdc25, and apoptosis proteins BAD and ASK-1 (3,4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: YWHAQ