CXCR4: Novus Biologicals

MAB172

CXCR4 Antibody (44716) [Unconjugated]

(2 Reviews)

100 μg

MAB172

MAB004, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox">

MAB004, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins." alt="Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human CXCR4 Monoclonal Antibody (Catalog # MAB172, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

CXCR4 was detected in immersion fixed paraffin-embedded sections of human lymph node using 15 µg/mL Human CXCR4 Monoclonal Antibody (Catalog # MAB172) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # <a class=

CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

CXCR4 was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human CXCR4 Monoclonal Antibody (Catalog # MAB172) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # <a class=

CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

350-NS) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF-1a (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCR4 Monoclonal Antibody (Catalog # MAB172). The ND₅₀ is typically 2.5-12 µg/mL." class="big_lightbox">

Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF-1a (Catalog # <a class=

350-NS) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF-1a (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCR4 Monoclonal Antibody (Catalog # MAB172). The ND₅₀ is typically 2.5-12 µg/mL." class="big_thumb" />

<b>Biological Strategies Validation. </b>CXCR4 neutralization leads to attenuation of the CD44+/CD133+ prostate progenitor population.(A) DU145 cells were plated in 96-well low-attachment plates at 100 cells per well (5 replicates) and the spheres were grown in serum-free, EBM medium with supplements. The antibodies were replenished daily. Cells were imaged with an Acumen eX3 microplate cytometer and spheres were detected using image analysis software. The sphere size was measured by GFP intensity. The spheres were discriminated from cell debris using a Gaussian filter. The spheres included in the analysis are outlined in red and indicated by arrows. Representative data from one of two independent experiments is shown; *- p value<0.05. (B) Flow cytometry analysis revealed attenuation of CD44+/CD133+ population in DU145 cells treated with 10 µg/ml neutralizing anti-CXCR4 (mouse monoclonal IgG, clone 44716, R&D Systems) or 10 µg/ml control antibody (mouse IgG isotype control, Lifespan Bioscience Inc.) for 5 days. The cell were grown in medium supplemented with 2% FBS. Culture medium was refreshed every second day; *- p value<0.05. (C) Western blot analysis of DU145 cells treated with 10 µg/ml neutralizing anti-CXCR4 antibody for 5 days demonstrated downregulation of the PI3K/AKT pathway activation compared to the cells treated with 10 µg/ml control antibody. The cell were grown in medium supplemented with 2% FBS. Culture medium was refreshed every second day. (D) Preincubation of prostate cancer cells with neutralizing anti-CXCR4 antibody significantly delays tumor growth. 5×105 DU145 cells pretreated with neutralizing anti-CXCR4 or control antibody for 5 days were embedded in BD matrigel and injected s.c. into NOD/SCID mice.*- p value<0.01. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0031226), licensed under a CC-BY license. Not internally tested by R&D Systems.

CXCR4 and CD133 are highly expressed in ovarian cancer patients. (A) Representative panel of immunohistochemistry for CD133 and CXCR4 expression (Magnification 200X; insert 400X). Non-homogeneous and focal cytoplasmic and membranous CD133 expression rated as negative (I), focally low (1–10% cancer cells) (II), focally high CD133 expression (>10% stained cancer cells) (III). Extensive homogeneous cytoplasmic and membranous, CXCR4 staining was reported rated as negative (IV) moderate (<50% of cancer cells) (V) and high expression (>50% of cancer cells) (VI). (B) QPCR analysis of CXCR4 and CD133 in freshly resected ovarian tumors and corresponding normal ovarian tissue. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep10357), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemical staining for MIF and CXCR4 in human esophageal carcinoma. Our data showed low expression levels of MIF (a) and CXCR4 (c) (X 400) and high expression levels of MIF (b) and CXCR4 (d), compared with the negative control (e) (X 400), in tumor tissues from patients with ESCC. The arrows point to the positive staining of tumor cells or TILs. Image collected and cropped by CiteAb from the following publication (//translational-medicine.biomedcentral.com/articles/10.1186/1479-5876-11-60), licensed under a CC-BY license. Not internally tested by R&D Systems.

Inflammatory markers. Sections of activated ovine spleen, isolated from a sheep with endocarditis. Hematoxylin and eosin (H&E, top left) staining reveals the characteristic splenic red pulp (R) and white pulp (W), with the central arterioles (CA). Sections are stained with antibodies against CD45, CD3, CD64, EGF-like module-containing mucin-like hormone receptor-like 1 (EMR-1), colony stimulating factor-1 receptor (CSF-1R), interleukin-10 (IL-10), inducible nitric oxide synthase (iNOS), CD163, CD200R, CD44, Arginase-1 (Arg-1), CCR-7, CXCR4, and tumor necrosis factor-alpha (TNF-alpha ). Scale bars, 200 μm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/30159315), licensed under a CC-BY license. Not internally tested by R&D Systems.

Knockdown of CXCR4 abrogates trastuzumab resistance. CXCR4 was silenced by specific shRNA in HCC1419 cells (“Materials and methods” section). The puromycin-resistant stable colonies were pooled together and named shCXCR4. A pool of cells infected with the lentivirus containing a non-effective vector (shRNA-NE) was selected and used as the control. A Western blot analysis was used to confirm the reduction in CXCR4 expression. B Illustration of co-cultures. C. BCAFs and HMFs were cultured in Dulbecco modified Eagle medium/nutrient mixture/F-12 supplemented with 10% FBS for 72 h. The culture supernatant was collected and tested for SDF-1 alpha using ELISA following the manufacturer’s instructions. The medium used for the cell culture was used as the negative control. The data were analyzed with one-way ANOVA. D, E CXCR4-knockdown cells or non-silent control cells were co-cultured with BCAFs (D) or with BCAFs and PBMCs (E) in 3D, followed by treatment with trastuzumab as illustrated in B. At the endpoint of the study, relative cell viability was quantitatively analyzed using CellTiter-Glo 3D viability assay kit. The data were analyzed with one-way ANOVA (*P < 0.05, **P < 0.01, ***P < 0.001 compared with the non-silent control cells). F, G CXCR4-knockdown cells or non-silent control cells were used for trastuzumab-induced antibody-dependent cellular cytotoxicity (detail in “Materials and methods” section). The cells were stained with propidium iodide and analyzed by flow cytometry (F). Data were analyzed using t-test analysis of variance and are reported as the mean ± SD of triplicates (G) Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37280713), licensed under a CC-BY license. Not internally tested by R&D Systems.

Targeting CXCR4 abrogates trastuzumab resistance. A HER2 + breast cancer cells with or without primary trastuzumab resistance were examined for CXCR4 expression with its antibody (UMB2, Abcam) using western blot analysis. The density of the bands was quantitatively analyzed. B, C Cell lines with high expression of CXCR4 (HCC1419 and HCC202) and low expression of CXCR4 (BT474 and SKBR3) were seeded in 3D Matrigel and treated with trastuzumab (B) or AMD3100 with SDF-1 alpha (4 ng/ml). (C). The total area of the acini was quantitatively analyzed (see “Materials and methods” section). D HCC1419 cells grown in 3D Matrigel culture were treated with trastuzumab (2 µg/ml), AMD3100 (1 µM), SDF-1 alpha (4 ng/ml), or the combination. Photographs were taken on day 13 after the start of treatment. The total area of the acini was quantitatively analyzed using AlphaView SA software (E). F–I Clonogenic assay. HCC1419 (F) and HCC202 (H) cells were seeded at low density and treated with AMD3100 (0.5 µM), SDF-1 alpha (4 ng/ml), trastuzumab (2.5 µg/ml), or the combination. The plates were scanned on day 18 after the start of treatment. Colony formation was quantitatively analyzed using AlphaView SA software. E, G, I Data were analyzed using one-way ANOVA and are reported as mean ± SD of triplicates, representing two independent experiments (*P < 0.0001 compared with vehicle) Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37280713), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB172U-100UG
Alexa Fluor 405	FAB172V-100UG
Alexa Fluor 488	FAB172G-100UG
Alexa Fluor 532	FAB172X
Alexa Fluor 594	FAB172T-100UG
Alexa Fluor 647	FAB172R-100UG
Alexa Fluor 700	FAB172N-100UG
Alexa Fluor 750	FAB172S-100UG
Alexa Fluor Plus 405	FAB172AFP405-100UG
Alexa Fluor Plus 488	FAB172AFP488-100UG
Alexa Fluor Plus 555	FAB172AFP555-100UG
Alexa Fluor Plus 594	FAB172AFP594-100UG
Alexa Fluor Plus 647	FAB172AFP647-100UG
Alexa Fluor Plus 680	FAB172AFP680-100UG
Allophycocyanin	FAB172A
Allophycocyanin/Cy7	FAB172APCCY7
CoraFluor 1	FAB172CL1
DyLight 350	FAB172D
DyLight 405	FAB172E
DyLight 488	FAB172K
DyLight 550	FAB172L
DyLight 594	FAB172M
DyLight 650	FAB172W
DyLight 680	FAB172Y
DyLight 755	FAB172Z
FITC	FAB172F
HRP	FAB172H
Janelia Fluor 525	FAB172JF525
Janelia Fluor 549	FAB172I
Janelia Fluor 646	FAB172J
Janelia Fluor 669	FAB172JF669
PE	FAB172P
PE/Atto594	FAB172PEATT594
PE/Cy5.5	FAB172PECY55
PE/Cy7	FAB172PECY7
PerCP	FAB172C
mFluor Violet 450 SE	FAB172MFV450
mFluor Violet 500 SE	FAB172MFV500
mFluor Violet 610 SE	FAB172MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB172-SP
Carrier Free	FAB172V-100UG
Carrier Free	FAB172U-100UG
Carrier Free	FAB172T-100UG
Carrier Free	FAB172S-100UG
Carrier Free	FAB172R-100UG
Carrier Free	FAB172N-100UG
Carrier Free	FAB172G-100UG

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MAB21651

CXCR4 Antibody (247506) [Unconjugated]

(1 Review)

(47 Publications)

100 μg

MAB21651

MAB0061) followed by anti-Rat IgG PE-conjugated Secondary Antibody (Catalog # F0105B) and Rat anti-Mouse CD8 alpha APC-conjugated Monoclonal Antibody (Catalog # FAB116A). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox">

MAB0061) followed by anti-Rat IgG PE-conjugated Secondary Antibody (Catalog # F0105B) and Rat anti-Mouse CD8 alpha APC-conjugated Monoclonal Antibody (Catalog # FAB116A). View our protocol for Staining Membrane-associated Proteins." alt="Mouse thymocytes were stained with (A) Rat Anti-Mouse CXCR4 Monoclonal Antibody (Catalog # MAB21651) or (B) Rat IgG2B isotype control antibody (Catalog # MAB0061) followed by anti-Rat IgG PE-conjugated Secondary Antibody (Catalog # F0105B) and Rat anti-Mouse CD8 alpha APC-conjugated Monoclonal Antibody (Catalog # FAB116A). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

CXCR4 was detected in immersion fixed mouse neural progenitor cells using 10 µg/mL Rat Anti-Mouse CXCR4 Monoclonal Antibody (Catalog # MAB21651) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # <a class=

NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

CXCR4 was detected in immersion fixed mouse splenocytes using 10 µg/mL Rat Anti-Mouse CXCR4 Monoclonal Antibody (Catalog # MAB21651) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # <a class=

NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

CTS017) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox">

CXCR4 was detected in immersion fixed frozen sections of mouse spleen using Rat Anti-Mouse CXCR4 Monoclonal Antibody (Catalog # MAB21651) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS017) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

460-SD) chemo-attracts the BaF3 mouse pro-B cell line transfected with mouse CXCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL12/ SDF-1a (10 ng/mL) is neutral-ized (green line) by increasing concentrations of Rat Anti-Mouse CXCR4 Monoclonal Antibody (Catalog # MAB21651). The ND₅₀ is typically 8-40 µg/mL." class="big_lightbox">

Recombinant Mouse CXCL12/SDF-1a (Catalog # <a class=

460-SD) chemo-attracts the BaF3 mouse pro-B cell line transfected with mouse CXCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL12/ SDF-1a (10 ng/mL) is neutral-ized (green line) by increasing concentrations of Rat Anti-Mouse CXCR4 Monoclonal Antibody (Catalog # MAB21651). The ND₅₀ is typically 8-40 µg/mL." class="big_thumb" />

<b>Biological Strategies Validation. </b>Effects of SDF-1-CXCR4/CXCR7 pathway on MSC chemotaxis in vitro.(A) The chemotaxis in response to SDF-1 alpha (10 ng/ml for 12 h) was performed in the NP-MSCs and HP-MSCs treated with a neutralizing anti-CXCR4 antibody, an anti-CXCR7 antibody, and the respective isotype-matched control antibodies. *P<0.05, vs NP-MSCs; †P<0.05, vs the respective isotype-matched control antibodies. (B) NP-MSCs were transiently overexpressed with CXCR4 using pORF9-mCXCR4 vector or with CXCR7 using pORF9-mCXCR7 vector (n = 6). A negative control empty (pORF9-MCS) vector was used. (C) The transfected cells were subjected to chemotaxis in response to the indicated concentrations of SDF-1 alpha for 12 h. *P<0.05, vs the empty vector. (D and E) Western blot analysis (D) and FCM (E) were performed to determine the intracellular and extracellular expression of both CXCR4 and CXCR7 in the cells treated with or without SDF-1 alpha (50 ng/ml for 60 min). (F) The chemotaxis in response to SDF-1 alpha was performed in the cells treated with or without SDF-1 alpha . Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22511954), licensed under a CC-BY license. Not internally tested by R&D Systems.

Downregulation of Cxcr4, SSEA1 and expression of TRA98 after release from GoST induction.(A) Immunofluorescence of Cxcr4 (white) and SSEA1 (green) after release from GoST induction. Cxcr4 and SSEA1 were detected simultaneously. DNA was counterstained with DAPI (blue). Cxcr4 and SSEA1 were expressed in ES, cES and GoST cells. Cxcr4 was downregulated at 4, 8 and 12 days after release from GoST induction, while SSEA1 remained expressed until 4 days after release from GoST induction and was downregulated afterwards at 8 and 12 days after release. Scalebar = 50 μm. (B) Immunofluorescence of TRA98 (white) and SSEA1 (green) after release from GoST induction. TRA98 and SSEA1 were detected simultaneously. DNA was counterstained with DAPI (blue). TRA98 and SSEA1 were expressed in ES, cES and GoST cells. Expression of TRA98 slightly decreased but remained detectable in the nucleus at 4, 8 and 12 days after release from GoST induction, while SSEA1 remained expressed until 4 days after release from GoST induction and was downregulated afterwards at 8 and 12 days after release. Scalebar = 50 μm. See Fig. S6. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep25104), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>CXCL12 induces CXCR4/CXCR7 and cell polarization in HUVECs. HUVECs were cultured on plastic Petri dishes (pretreated with PDL) with BSA stripes (the control group) and BSA plus CXCL12 stripes (CXCL12 group) for 5 min. HUVECs were fixed and stained with antibodies against CXCR4 (purple), CXCR7 (blue) and F-Actin (red). The micro stripes of fluorescein-conjugated BSA (A) or CXCL12 (H) are shown. CXCR4 (B), CXCR7 (C) and F-Actin (D) in HUVECs cultured on BSA stripes stayed in the resting state, while the polarization of CXCR4 (arrow: I), CXCR7 (arrow: J) and F-Actin (arrow: K) was observed in HUVECs cultured on CXCL12 stripes for 5 min. Moreover, the HUVECs in the BSA control group showed no morphological changes (E–G). The CXCL12 group HUVECs shows polarization towards the CXCL12 stripe (L–N). Scale bar: 10 μm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28811579), licensed under a CC-BY license. Not internally tested by R&D Systems.

RhoA modulates the CXCR4-CXCL12 axis in breast tumors. (a) Representative immunohistochemical (IHC) staining images of breast primary tumor sections of mice orthotopically injected with 4T1 GFP-LUC cells carrying indicated lentiviral modifications, stained with antibodies for CD31 (top, left), F4/80 (middle, left) and alpha -SMA (bottom, left). CD31+ area (top, right), F4/80+ cells (middle, right) and alpha -SMA+ area (bottom, right) per tumor field of view (FOV) (mean ± SE) quantifications of IHC images. N = 4. (b) Representative IHC staining images of breast primary tumor sections of above mice stained with CXCL12 antibody (left). CXCL12+ area per tumor FOV (mean ± SE) quantification of IHC images. N = 4. (c) Representative immunofluorescent images of breast primary tumor sections of above mice co-stained with alpha -SMA, CXCL12 and DRAQ5 nuclear stain, showing the co-localization of alpha -SMA and CXCL12 signals. Scale bar: 50 µm. (d) Representative IHC staining images of breast primary tumor sections of above mice stained with CXCR4 antibody (left). CXCR4+ area per tumor FOV (mean ± SE) quantification of IHC images. N = 4. (e) Relative mCXCR4 mRNA levels normalized with mActin (mean ± SE), as quantified by qRT-PCR in primary breast tumors of above mice (top) and in 4T1 GFP-LUC cells carrying indicated lentiviral modifications (bottom). N = 4, ns- not significant *P < 0.05, unpaired Student’s t-test (two-tailed). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31705019), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, , , ,

Applications: , , , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB21651U-100UG
Alexa Fluor 405	FAB21651V-100UG
Alexa Fluor 488	FAB21651G-100UG
Alexa Fluor 532	FAB21651X
Alexa Fluor 594	FAB21651T-100UG
Alexa Fluor 647	FAB21651R-100UG
Alexa Fluor 700	FAB21651N-100UG
Alexa Fluor 750	FAB21651S-100UG
Alexa Fluor Plus 405	FAB21651AFP405-100UG
Alexa Fluor Plus 488	FAB21651AFP488-100UG
Alexa Fluor Plus 555	FAB21651AFP555-100UG
Alexa Fluor Plus 594	FAB21651AFP594-100UG
Alexa Fluor Plus 647	FAB21651AFP647-100UG
Alexa Fluor Plus 680	FAB21651AFP680-100UG
Allophycocyanin	FAB21651A-025UG
Allophycocyanin	FAB21651A-100UG
Allophycocyanin/Cy7	FAB21651APCCY7
Biotin	FAB21651B
CoraFluor 1	FAB21651CL1
DyLight 350	FAB21651D
DyLight 405	FAB21651E
DyLight 488	FAB21651K
DyLight 550	FAB21651L
DyLight 594	FAB21651M
DyLight 650	FAB21651W
DyLight 680	FAB21651Y
DyLight 755	FAB21651Z
Fluorescein	FAB21651F-100
Fluorescein	FAB21651F-025
HRP	FAB21651H
Janelia Fluor 525	FAB21651JF525
Janelia Fluor 549	FAB21651I
Janelia Fluor 646	FAB21651J
Janelia Fluor 669	FAB21651JF669
PE/Atto594	FAB21651PEATT594
PE/Cy5.5	FAB21651PECY55
PE/Cy7	FAB21651PECY7
Phycoerythrin	FAB21651P-100
Phycoerythrin	FAB21651P-025
mFluor Violet 450 SE	FAB21651MFV450
mFluor Violet 500 SE	FAB21651MFV500
mFluor Violet 610 SE	FAB21651MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB21651F-025
Carrier Free	FAB21651F-100
Carrier Free	FAB21651P-025
Carrier Free	FAB21651P-100
Carrier Free	MAB21651-SP
Carrier Free	MAB21651-500
Carrier Free	FAB21651U-100UG
Carrier Free	FAB21651T-100UG
Carrier Free	FAB21651S-100UG
Carrier Free	FAB21651R-100UG
Carrier Free	FAB21651N-100UG
Carrier Free	FAB21651G-100UG
Carrier Free	FAB21651V-100UG
Carrier Free	FAB21651A-100UG
Carrier Free	FAB21651A-025UG

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NB100-74396

CXCR4 Antibody - BSA Free

(1 Review)

(30 Publications)

0.1 ml

NB100-74396

<b>Biological Strategies Validation. </b>CXCR4-Antibody-Immunohistochemistry-NB100-74396-img0010.jpg

<b>Biological Strategies Validation. </b>CXCR4-Antibody-Immunohistochemistry-NB100-74396-img0011.jpg

Western Blot: CXCR4 Antibody [NB100-74396] - Analysis of CXCR4 in HeLa whole cell extract.

Western Blot: CXCR4 Antibody [NB100-74396] - Analysis of CXCR4 protein in human small intestine tissue lysate using 1:500 dilution of rabbit polyclonal CXCR4 antibody (Lot A2). The signal was developed using ECL method and the antibody generated a specific band of CXCR4 at ~40 kDa position.

Immunocytochemistry/Immunofluorescence: CXCR4 Antibody [NB100-74396] - CXCR4 antibody was tested in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).

Immunohistochemistry: CXCR4 Antibody [NB100-74396] - Immunostaining of CXCR4 in human cervical carcinoma tissue sections.

Immunocytochemistry/ Immunofluorescence: CXCR4 Antibody [NB100-74396] - The A2AR & baicalin attenuated CXCR4 expression in the hypoxia-induced PAH mouse model. CXCR4 & MYH11 expression levels in mouse PASMCs were analyzed by immunofluorescence staining (a; n = 3). CXCR4 protein is stained red, & MYH11 is stained green to indicate the PASMCs (×400; scale bars indicate 50 μm). CXCR4 fluorescence intensity was calculated (b; n = 3). CXCR4 protein expression levels in lung tissue were examined by western blot. GAPDH served as an internal control (c, d; n = 3). Data are presented as the mean ± SD. #Value significantly greater than the corresponding value in saline-treated normoxic mice (##P < 0.01). *Value significantly less than the corresponding value in hypoxic mice (*P < 0.05, **P < 0.01). §Value significantly less than the corresponding value in baicalin-treated mice (§P < 0.05, §§P < 0.01). +Value significantly different between WT & A2AR−/− mice (+P < 0.05, ++P < 0.01). WTH, wild-type hypoxic; A2AR−/−H, A2AR−/− hypoxic; s, saline-treated Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28774332), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

$Immunocytochemistry/ Immunofluorescence: CXCR4 Antibody [NB100-74396] - Isolation of prostate CTCs from metastatic PCa patients using anti-CD45 immunomagnetic depletion.2.5 ml blood from three metastatic PCa patients (> 50 CTCs/ 2.5 ml blood) was processed via ficoll density centrifugation & the PBMC fraction was collected. Immunomagnetic anti-CD45 depletion was performed on the obtained PBMCs & the remaining cells were washed, cytospunned onto the slides. Slides were stained for PSMA, EpCAM, sLex, & CXCR4 using the protocol as described in Figure S1. MDA, PC3, & KG1 cells were simultaneously stained as a control for the following markers: PSMA= Magenta, EpCAM= Yellow, HECA-452= Green, CXCR4= Red. All prostate CTCs expressed CXCR4, while, sLex expression was variable. The analysis of sLex intensity is shown in Figure 4. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24386459), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-74396SS

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MAB170

CXCR4 Antibody (12G5) [Unconjugated]

(43 Publications)

500 μg

MAB170

FAB4867A) and either (A) Mouse Anti-Human CXCR4 Monoclonal Antibody (Catalog # MAB170) or (B) Mouse IgG_2AIsotype Control (Catalog # MAB003) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox">

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # <a class=

FAB4867A) and either (A) Mouse Anti-Human CXCR4 Monoclonal Antibody (Catalog # MAB170) or (B) Mouse IgG_2AIsotype Control (Catalog # MAB003) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." alt="Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Mouse Anti-Human CXCR4 Monoclonal Antibody (Catalog # MAB170) or (B) Mouse IgG_2AIsotype Control (Catalog # MAB003) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

350-NS) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human/Feline/ Rhesus Macaque CXCL12/ SDF-1a (1 ng/mL) is neutralized (green line) by increasing concentrations of Human CXCR4 Monoclonal Antibody (Catalog # MAB170). The ND₅₀ is typically 0.3-1.2 µg/mL." class="big_lightbox">

350-NS) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human/Feline/ Rhesus Macaque CXCL12/ SDF-1a (1 ng/mL) is neutralized (green line) by increasing concentrations of Human CXCR4 Monoclonal Antibody (Catalog # MAB170). The ND₅₀ is typically 0.3-1.2 µg/mL." class="big_thumb" />

F0116). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox">

Jurkat (filled histogram) vs K562 (open histogram) cells were stained with Mouse Anti-Human CXCR4 Monoclonal Antibody followed by Phycoerythrin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # <a class=

F0116). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

Effect of hypoxia on CX3CR1 and CXCR4 expression by early culture of MSCs.(A) The MSCs were cultured for 4–20 h in normoxic (Nor) or hypoxic (Hyp) conditions, as indicated. Total RNA was analyzed by RT-PCR for CX3CR1, CX3CR4, and b-actin mRNA expression. (B) Total RNA from MSCs cultured for 20 h in the presence of increasing concentrations of DFX was analyzed by RT-PCR for CX3CR1 and CXCR4 mRNA expression. (C) Quantitaive RT-PCR for CXCR4 and CX3CR1 mRNA expression at 20 h of treatment. The vertical axis represents the relative ratio of indicated condition to normoxic condition without the addition of DFX.. (D) Surface expression of CXCR4 and CX3CR1 were determined by flow cytometry using a mouse mAb anti-CXCR4 and a rat mAb anti-CX3CR1, respectively (continuous line). Irrelevant antibody is indicated by the dotted line. The results are representative of three independent experiments. (E) Hypoxia and DFX-treated MSCs were cultured for 24 h in the indicated conditions and analyzed by western blot for HIF-1 alpha , CXCR4, CX3CR1, and actin protein levels. (F) HIF-1 alpha binding to the CX3CR1 promoter. The MSCs were cultured for 4 h in normoxic or hypoxic condition. ChIP was performed with or without rabbit antibody specific for HIF-1 alpha . There is no difference between PCR of the CX3CR1 promoter using input chromatin for normoxic and hypoxic cells. However, a band was noted for hypoxic cells but not for normoxic cells, when PCR of the CX3CR1 promoter was performed using immunoprecipitation with HIF-1 alpha antibody. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/17476338), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB170U-100UG
Alexa Fluor 405	FAB170V-100UG
Alexa Fluor 488	FAB170G-025
Alexa Fluor 488	FAB170G-100
Alexa Fluor 532	FAB170X
Alexa Fluor 594	FAB170T-100UG
Alexa Fluor 647	FAB170R-100UG
Alexa Fluor 750	FAB170S-100UG
Alexa Fluor Plus 405	FAB170AFP405-100UG
Alexa Fluor Plus 488	FAB170AFP488-100UG
Alexa Fluor Plus 555	FAB170AFP555-100UG
Alexa Fluor Plus 594	FAB170AFP594-100UG
Alexa Fluor Plus 647	FAB170AFP647-100UG
Alexa Fluor Plus 680	FAB170AFP680-100UG
Allophycocyanin	FAB170A-025
Allophycocyanin	FAB170A-100
Allophycocyanin/Cy7	FAB170APCCY7
Biotin	FAB170B
CoraFluor 1	FAB170CL1
DyLight 350	FAB170D
DyLight 405	FAB170E
DyLight 488	FAB170K
DyLight 550	FAB170L
DyLight 594	FAB170M
DyLight 650	FAB170W
DyLight 680	FAB170Y
DyLight 755	FAB170Z
Fluorescein	FAB170F-100
Fluorescein	FAB170F-025
HRP	FAB170H
Janelia Fluor 525	FAB170JF525
Janelia Fluor 549	FAB170I
Janelia Fluor 646	FAB170J
Janelia Fluor 669	FAB170JF669
PE/Atto594	FAB170PEATT594
PE/Cy5.5	FAB170PECY55
PE/Cy7	FAB170PECY7
Phycoerythrin	FAB170P-100
Phycoerythrin	FAB170P-025
mFluor Violet 450 SE	FAB170MFV450
mFluor Violet 500 SE	FAB170MFV500
mFluor Violet 610 SE	FAB170MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB170F-025
Carrier Free	FAB170F-100
Carrier Free	FAB170P-025
Carrier Free	FAB170P-100
Carrier Free	FAB170A-025
Carrier Free	FAB170A-100
Carrier Free	FAB170B
Carrier Free	FAB170G-100
Carrier Free	FAB170G-025
Carrier Free	MAB170-SP
Carrier Free	FAB170V-100UG
Carrier Free	FAB170U-100UG
Carrier Free	FAB170T-100UG
Carrier Free	FAB170S-100UG
Carrier Free	FAB170R-100UG

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NB100-56437

CXCR4 Antibody - BSA Free

(1 Review)

(22 Publications)

0.1 mg

NB100-56437

Immunocytochemistry/Immunofluorescence: CXCR4 Antibody [NB100-56437] - Jurkat cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-CXCR4 (NB100-56437) at a 1:25 dilution for 1 hour at room temperature and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with and anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry-Paraffin: CXCR4 Antibody [NB100-56437] - CXCR4 was detected in immersion sections of human tonsil using rabbit anti-human antibody (Catalog # NB100-56437) at 1:300 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-rabbit HRP polymer detection reagent with DAB chromogen (brown) and counterstained with hematoxylin (blue).

CXCL12 secreted from DFs induces AR and CXCR4 in DPCs. The effects of rCXCL12 on the expression of AR and CXCR4 in DPCs were observed using qRT-PCR (A,B) and Western blot analysis (C). rCXCL12 increased the mRNA and protein expression of the AR and CXCR4 in DPCs. $ p < 0.05, $$ p < 0.01 vs. Control. (D) DFs were treated with 100 nM TP or DHT for 48 h and the culture medium (CM) was collected. CM from DFs treated with TP and DHT (DFCMTP and DFCMDHT) significantly reduced hair length in human hair organ culture. $ p < 0.05 vs. DFCM, n = 10. The dollar sign ($) indicates differences in a one-way ANOVA. Image collected and cropped by CiteAb from the following open publication (//www.mdpi.com/1422-0067/25/3/1705), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Sh

Species Glossary

Reactivity: Hu, Mu, Rt, Sh

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-537

Alt Catalog# IMG-537

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-24862
BSA Free	NB100-56437SS

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MAB173

CXCR4 Antibody (44717) [Unconjugated]

(31 Publications)

100 μg

MAB173

350-NS) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF-1a (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCR4 Monoclonal Antibody (Catalog # MAB173). The ND₅₀ is typically 1-5 µg/mL." class="big_lightbox">

350-NS) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF-1a (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCR4 Monoclonal Antibody (Catalog # MAB173). The ND₅₀ is typically 1-5 µg/mL." alt="Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF-1a (Catalog # 350-NS) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF-1a (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCR4 Monoclonal Antibody (Catalog # MAB173). The ND₅₀ is typically 1-5 µg/mL." class="big_thumb" />

Ectopic expression of CXCR7 and CXCR4 on MDCK cells.(A) MDCK were stably transfected with empty vector (Mock), with CXCR7, CXCR4, a vector coding for a CXCR7 lacking the cytoplasmic C-terminus ( delta CXCR7), and a vector coding for a chimeric CXCR7 containing the DRYLAIV motive of CXCR4. Receptor expression was determined by FACS analysis using saturating antibody concentrations (see Methods). (B) Confocal immunofluorescence analysis of unfixed MDCK cells expressing CXCR7 (upper panels) or CXCR4 (lower panels). Cells also expressed N-ter-Lck mCherry as membrane marker (red fluorescence). Left panels: confocal images of planes cut through intracellular regions of MDCK monolayers. Right panels: x-z planes reconstructed from confocal x-y stacks. For receptor (green) detection anti-CXCR7 (11G8 R&D) or anti CXCR4 (MAB173 R&D) were used. Receptor-bound primary antibodies were revealed with goat anti mouse IgG conjugated with Alexa488 (green fluorescence). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/20161793), licensed under a CC-BY license. Not internally tested by R&D Systems.

In vitro cell invasion is stimulated by the SDF-1/CXCR4 pathway independently from HPV status.The modulation of E6 expression was monitored using Western-blot in HPV-positive HeLa (A) and TC-1 (B) cells and in HPV-negative B16F10 (C) cells, after 3 and 6 days of incubation with Cidofovir (CDF). Modulation of P53 expression was assessed in HeLa cells after CDF incubation (A). Cell invasion was measured using a Matrigel assay in HeLa (A), TC-1 (B) and B16F10 (C) cells. Recombinant human CXCL12/SDF-1 (100 ng/mL; R&D Systems) was used as chemoattractant and modulation of cell migration was recorded after treatment with CXCR4-blocking antibody or/and Cidofovir (CDF). The invasion rate was determined by counting crystal violet-stained cells. Invasion was stimulated by SDF-1 alpha /CXCR4 independently from the HPV status of the cells but Cidofovir anti-invasive action was restricted to the two HPV-positive cell lines. Three independent experiments with three chambers each time were performed. ***P<0.01, for a statistically true difference, as compared to the untreated group. # P<0.01 compared to SDF-1 alpha -treated group. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/19325708), licensed under a CC-BY license. Not internally tested by R&D Systems.

TC-1 cell lung metastasis is mediated by the CXCR4 in vivo.TC-1 cells (1×106) were injected into mice tail veins either without or with pre-treatment with CXCR4-blocking antibody (2B11), irrelevant antibody (CD71), or trypsin/EDTA. (A). Representative Hematoxylin-Eosin-Saffranin (HES)-satined sections of lungs of mice are shown. Digitization of the whole slides was performed for each animal/treatment type. Histopathological analysis was done after delineation of metastases (dashed line) vs whole lung area (B) on each HES-stained section with the ImageJ software. The lung metastasis indices within each cohort of mice are expressed as fold decrease (+/−SEM) over untreated controls ***P<0.01. (C) Representative sections of CXCR4 immunostaining are shown. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/19325708), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB173U-100UG
Alexa Fluor 405	FAB173V-100UG
Alexa Fluor 488	FAB173G-100
Alexa Fluor 488	FAB173G-025
Alexa Fluor 532	FAB173X
Alexa Fluor 594	FAB173T-100UG
Alexa Fluor 647	FAB173R-100UG
Alexa Fluor 700	FAB173N-100UG
Alexa Fluor 750	FAB173S-100UG
Alexa Fluor Plus 405	FAB173AFP405-100UG
Alexa Fluor Plus 488	FAB173AFP488-100UG
Alexa Fluor Plus 555	FAB173AFP555-100UG
Alexa Fluor Plus 594	FAB173AFP594-100UG
Alexa Fluor Plus 647	FAB173AFP647-100UG
Alexa Fluor Plus 680	FAB173AFP680-100UG
Allophycocyanin	FAB173A-025
Allophycocyanin	FAB173A-100
Allophycocyanin/Cy7	FAB173APCCY7
CoraFluor 1	FAB173CL1
DyLight 350	FAB173D
DyLight 405	FAB173E
DyLight 488	FAB173K
DyLight 550	FAB173L
DyLight 594	FAB173M
DyLight 650	FAB173W
DyLight 680	FAB173Y
DyLight 755	FAB173Z
FITC	FAB173F
HRP	FAB173H
Janelia Fluor 525	FAB173JF525
Janelia Fluor 549	FAB173I
Janelia Fluor 646	FAB173J
Janelia Fluor 669	FAB173JF669
PE/Atto594	FAB173PEATT594
PE/Cy5.5	FAB173PECY55
PE/Cy7	FAB173PECY7
Phycoerythrin	FAB173P-100
Phycoerythrin	FAB173P-025
mFluor Violet 450 SE	FAB173MFV450
mFluor Violet 500 SE	FAB173MFV500
mFluor Violet 610 SE	FAB173MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB173A-025
Carrier Free	FAB173A-100
Carrier Free	FAB173G-025
Carrier Free	FAB173G-100
Carrier Free	FAB173P-025
Carrier Free	FAB173P-100
Carrier Free	MAB173-SP
Carrier Free	FAB173V-100UG
Carrier Free	FAB173U-100UG
Carrier Free	FAB173T-100UG
Carrier Free	FAB173S-100UG
Carrier Free	FAB173R-100UG
Carrier Free	FAB173N-100UG

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MAB171

CXCR4 Antibody (44708) [Unconjugated]

(23 Publications)

100 μg

MAB171

350-NS) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF-1a (1 ng/mL) is neutralized (green line) by increasing concentrations of Human CXCR4 Monoclonal Antibody (Catalog # MAB171). The ND₅₀ is typically 0.2-1.2 µg/mL." class="big_lightbox">

350-NS) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF-1a (1 ng/mL) is neutralized (green line) by increasing concentrations of Human CXCR4 Monoclonal Antibody (Catalog # MAB171). The ND₅₀ is typically 0.2-1.2 µg/mL." alt="Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF-1a (Catalog # 350-NS) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF-1a (1 ng/mL) is neutralized (green line) by increasing concentrations of Human CXCR4 Monoclonal Antibody (Catalog # MAB171). The ND₅₀ is typically 0.2-1.2 µg/mL." class="big_thumb" />

<b>Biological Strategies Validation. </b>Anti-CXCR4 antibody reduces CXCR4 surface expression, and blocks migration toward CXCL12 and BMSCs.(A) Relative MFIR of CXCR4 surface staining after incubation of Raji transfectants with 100 µg/mL of anti-CXCR4 antibody for 30 minutes. (B) Flow cytometry histograms showing the reduction in CXCR4 expression after incubation with neutralizing anti-CXCR4 antibody. White histograms represent negative unstained controls. (C) CXCR4-blocked cells were stimulated with 100 ng/mL of CXCL12 for 5 minutes and activation of Akt and ERK1/2 proteins analyzed by western blotting. (D) CXCR4-blocked cells were subjected to migration assay for 4 hours at 37°C in 5% CO2 toward 100 ng/mL of CXCL12 (left panel) or the stromal MS-5 cell line (right panel). Cells in the lower chamber were counted by flow cytometry under a defined flow rate for 5 minutes. Number of migrated cells treated with isotypic control was considered 100%. Results are shown as the mean ± SEM of 4 independent experiments. (E) Cell viability was measured by Annexin V-PI staining in cells incubated with 100 µg/mL of anti-CXCR4 or isotypic control for 48 hours. Results are shown as the mean ± SEM of 4 independent experiments Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0081221), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB171U-100UG
Alexa Fluor 405	FAB171V-100UG
Alexa Fluor 488	FAB171G-100UG
Alexa Fluor 532	FAB171X-100UG
Alexa Fluor 594	FAB171T-100UG
Alexa Fluor 647	FAB171R-100UG
Alexa Fluor 700	FAB171N-100UG
Alexa Fluor 750	FAB171S-100UG
Alexa Fluor Plus 405	FAB171AFP405-100UG
Alexa Fluor Plus 488	FAB171AFP488-100UG
Alexa Fluor Plus 555	FAB171AFP555-100UG
Alexa Fluor Plus 594	FAB171AFP594-100UG
Alexa Fluor Plus 647	FAB171AFP647-100UG
Alexa Fluor Plus 680	FAB171AFP680-100UG

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB171-SP

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NBP1-77067

CXCR4 Antibody - BSA Free

(5 Publications)

0.1 mg

NBP1-77067

<b>Genetic Strategies Validation. </b>Flow Cytometry: CXCR4 Antibody [NBP1-77067] - Astrocytes from wild-type or CXCR4 knockout mice were stained with primary antibodies against CXCR4 and FITClabeled secondary antibodies, and subsequently subjected to flow cytometry. CXCR4-/- astrocytes (red) showed loss of CXCR4 cell-surface expression compared with wild-type cells (black).

Western Blot: CXCR4 Antibody [NBP1-77067] - HeLa whole cell lysate with CXCR4 antibody at 0.5 ug/ml.

Western Blot: CXCR4 Antibody [NBP1-77067] - Recombinant Protein Test: Loading: CXCR4 partial recombinant protein (H00007852-Q01). Lane 1: Anti-CXCR4 antibody (0.1 ug/mL) 1 h incubation at RT in 5% NFDM/TBST. Lane 2: Coomassie blue staining.

Western Blot: CXCR4 Antibody [NBP1-77067] - CXCR4 protein was detected in the human metastatic melanoma cell lines and human melanoma cell line (colo38), but not in the human primary melanocytes (MPR1) with anti-CXCR4 antibodies.

Western Blot: CXCR4 Antibody [NBP1-77067] - U87MG and U87MG-CXCR4 extracts were included as negative and positive controls, respectively, for CXCR4 detection with anti-CXCR4 antibodies.

Western Blot: CXCR4 Antibody [NBP1-77067] - Loading: Lysates/proteins at 20 ug per lane. Antibodies: NBP1-7706 (2 ug/mL) or NBP1-76480 (2 ug/mL). 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

<b>Genetic Strategies Validation. </b>Western Blot: CXCR4 Antibody [NBP1-77067] - HeLa cells were transfected with control siRNAs (lane 1) or CXCR4 siRNAs (lane 2) Loading: 10 ug of HeLa whole cell lysates per lane. Antibodies: NBP1-7706 (2 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat antirabbit IgG HRP conjugate at 1:10000 dilution.

Flow Cytometry: CXCR4 Antibody [NBP1-77067] - Analysis of HeLa cells using CXCR4 antibody at 0.1 ug/ml. Purple: cells without staining, Green: Isotype control. Red : CXCR4 antibody.

Immunocytochemistry/ Immunofluorescence: CXCR4 Antibody - BSA Free [NBP1-77067] - Validation of CXCR4 in HeLa Cells. Immunocytochemical analysis of HeLa cells using anti-CXCR4 antibody at 2 u/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4;C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunocytochemistry/ Immunofluorescence: CXCR4 Antibody - BSA Free [NBP1-77067] - Validation of CXCR4 in HeLa Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling CXCR4 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing both membrane and cytoplasmic staining on HeLa cells.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-125-1

Alt Catalog# IMG-125-1

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-77067AF350
Alexa Fluor 405	NBP1-77067AF405
Alexa Fluor 488	NBP1-77067AF488
Alexa Fluor 532	NBP1-77067AF532
Alexa Fluor 594	NBP1-77067AF594
Alexa Fluor 647	NBP1-77067AF647
Alexa Fluor 700	NBP1-77067AF700
Alexa Fluor 750	NBP1-77067AF750
Alexa Fluor Plus 405	NBP1-77067AFP405
Alexa Fluor Plus 488	NBP1-77067AFP488
Alexa Fluor Plus 555	NBP1-77067AFP555
Alexa Fluor Plus 594	NBP1-77067AFP594
Alexa Fluor Plus 647	NBP1-77067AFP647
Alexa Fluor Plus 680	NBP1-77067AFP680
Allophycocyanin	NBP1-77067APC
Biotin	NBP1-77067B
CoraFluor 1	NBP1-77067CL1
DyLight 350	NBP1-77067UV
DyLight 405	NBP1-77067V
DyLight 488	NBP1-77067G
DyLight 550	NBP1-77067R
DyLight 594	NBP1-77067DL594
DyLight 650	NBP1-77067C
DyLight 680	NBP1-77067FR
DyLight 755	NBP1-77067IR
FITC	NBP1-77067F
HRP	NBP1-77067H
Janelia Fluor 525	NBP1-77067JF525
Janelia Fluor 549	NBP1-77067JF549
Janelia Fluor 646	NBP1-77067JF646
Janelia Fluor 669	NBP1-77067JF669
PE	NBP1-77067PE
PerCP	NBP1-77067PCP
mFluor Violet 450 SE	NBP1-77067MFV450
mFluor Violet 500 SE	NBP1-77067MFV500
mFluor Violet 610 SE	NBP1-77067MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-77067SS

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NBP1-76480

CXCR4 Antibody - BSA Free

(1 Publication)

0.1 mg

NBP1-76480

Immunohistochemistry-Paraffin: CXCR4 Antibody [NBP1-76480] - Staining of CXCR4 in mouse spleen tissue with CXCR4 antibody at 5 ug/ml.

Western Blot: CXCR4 Antibody [NBP1-76480] - HeLa total cell lysate.

<b>Genetic Strategies Validation. </b>Western Blot: CXCR4 Antibody [NBP1-76480] - Validation with CXCR4 siRNA Knockdown. HeLa cells were transfected with control siRNAs (lane 1) or CXCR4 siRNAs (lane 2) Loading: 10 ug of HeLa whole cell lysates per lane. Antibodies: NBP1-76480 (2 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate.

Reactivity:

Hu, Mu, Rt, Po

Species Glossary

Reactivity: Hu, Mu, Rt, Po

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-76480AF350
Alexa Fluor 405	NBP1-76480AF405
Alexa Fluor 488	NBP1-76480AF488
Alexa Fluor 532	NBP1-76480AF532
Alexa Fluor 594	NBP1-76480AF594
Alexa Fluor 647	NBP1-76480AF647
Alexa Fluor 700	NBP1-76480AF700
Alexa Fluor 750	NBP1-76480AF750
Allophycocyanin	NBP1-76480APC
Biotin	NBP1-76480B
CoraFluor 1	NBP1-76480CL1
DyLight 350	NBP1-76480UV
DyLight 405	NBP1-76480V
DyLight 488	NBP1-76480G
DyLight 550	NBP1-76480R
DyLight 594	NBP1-76480DL594
DyLight 650	NBP1-76480C
DyLight 680	NBP1-76480FR
DyLight 755	NBP1-76480IR
FITC	NBP1-76480F
HRP	NBP1-76480H
Janelia Fluor 525	NBP1-76480JF525
Janelia Fluor 549	NBP1-76480JF549
Janelia Fluor 646	NBP1-76480JF646
Janelia Fluor 669	NBP1-76480JF669
PE	NBP1-76480PE
PerCP	NBP1-76480PCP
mFluor Violet 450 SE	NBP1-76480MFV450
mFluor Violet 500 SE	NBP1-76480MFV500
mFluor Violet 610 SE	NBP1-76480MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-76480-0.025mg

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NBP1-77560

CXCR4 Antibody - BSA Free

(2 Publications)

0.1 ml

NBP1-77560

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-77560AF350
Alexa Fluor 405	NBP1-77560AF405
Alexa Fluor 488	NBP1-77560AF488
Alexa Fluor 532	NBP1-77560AF532
Alexa Fluor 594	NBP1-77560AF594
Alexa Fluor 647	NBP1-77560AF647
Alexa Fluor 700	NBP1-77560AF700
Alexa Fluor 750	NBP1-77560AF750
Alexa Fluor Plus 405	NBP1-77560AFP405
Alexa Fluor Plus 488	NBP1-77560AFP488
Alexa Fluor Plus 555	NBP1-77560AFP555
Alexa Fluor Plus 594	NBP1-77560AFP594
Alexa Fluor Plus 647	NBP1-77560AFP647
Alexa Fluor Plus 680	NBP1-77560AFP680
Allophycocyanin	NBP1-77560APC
Biotin	NBP1-77560B
CoraFluor 1	NBP1-77560CL1
DyLight 350	NBP1-77560UV
DyLight 405	NBP1-77560V
DyLight 488	NBP1-77560G
DyLight 550	NBP1-77560R
DyLight 594	NBP1-77560DL594
DyLight 650	NBP1-77560C
DyLight 680	NBP1-77560FR
DyLight 755	NBP1-77560IR
FITC	NBP1-77560F
HRP	NBP1-77560H
Janelia Fluor 525	NBP1-77560JF525
Janelia Fluor 549	NBP1-77560JF549
Janelia Fluor 646	NBP1-77560JF646
Janelia Fluor 669	NBP1-77560JF669
PE	NBP1-77560PE
PerCP	NBP1-77560PCP
mFluor Violet 450 SE	NBP1-77560MFV450
mFluor Violet 500 SE	NBP1-77560MFV500
mFluor Violet 610 SE	NBP1-77560MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-77560SS

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NLS1380

CXCR4 Antibody - BSA Free

(1 Review)

(5 Publications)

0.05 ml

NLS1380

Immunohistochemistry-Paraffin: CXCR4 Antibody [NLS1380] - Analysis of anti-CXCR4 antibody with human colon, carcinoma.

Immunohistochemistry-Paraffin: CXCR4 Antibody [NLS1380] - Human Lymph Node, Non-Hodgkins Lymphoma formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

<b>Biological Strategies Validation. </b>Immunohistochemistry-Frozen: CXCR4 Antibody [NLS1380] - Rat brain tissue sections. Rat Nucleus Accumbens treated with MDPV. Images taken with fluorescent microscope. Primary antibody was diluted to 1:200. Secondary antibody used was donkey anti-Rabbit Alexa Fluor 594 at 1:500. IHC-Fr image submitted by a verified customer review.

Immunohistochemistry-Paraffin: CXCR4 Antibody [NLS1380] - Lung, small cell carcinoma

Reactivity:

Hu, Mu, Rt, Ch, Ha, Pm, Pm, Rb, Pm, Ma-Op, Pm-Or, Tr

Species Glossary

Reactivity: Hu, Mu, Rt, Ch, Ha, Pm, Pm, Rb, Pm, Ma-Op, Pm-Or, Tr

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP3-06841

Human CXCR4 ELISA Kit (Colorimetric)

1 Kit

NBP3-06841

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Standard Curve:

78.13 - 5000 pg/mL

Sensitivity:

46.88 pg/mL

Sample Type:

Serum, plasma, cell supernatant, and other biological fluids

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

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