CCL2/JE/MCP-1: Novus Biologicals

DCP00

Human CCL2/MCP-1 Quantikine ELISA Kit

(271 Publications)

1 Kit

DCP00

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Conjugate	Catalog #	Availability	Size	Price
HRP	PDCP00
HRP	SCP00

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DY479

Mouse CCL2/JE/MCP-1 DuoSet ELISA, 15 Plate

(182 Publications)

1 Kit (for 5 Plates)

DY479

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

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DY279

Human CCL2/MCP-1 DuoSet ELISA, 15 Plate

(165 Publications)

1 Kit (for 5 Plates)

DY279

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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279-MC

Recombinant Human CCL2/MCP-1 Protein

(103 Publications)

10 μg

279-MC

1 μg/lane of Recombinant Human CCL2/MCP-1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 10 kDa.

MALDI-TOF analysis of Recombinant Human CCL2/MCP-1. The major peak corresponds to the calculated molecular mass, 8679 Da. The minor peak at 8887 is a matrix-associated artifact of the MALDI-TOF.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	279-MC-001MG/CF
Carrier Free	279-MC-010/CF
Carrier Free	279-MC-050/CF

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479-JE

Recombinant Mouse CCL2/JE/MCP-1 Protein

(100 Publications)

10 μg

479-JE

1 μg/lane of Recombinant Mouse CCL2/JE/MCP-1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 8 kDa.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	479-JE-010/CF
Carrier Free	479-JE-050/CF

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AF-479-NA

CCL2/JE/MCP-1 Antibody [Unconjugated]

(2 Reviews)

(63 Publications)

100 μg

AF-479-NA

479-JE), Recombinant Human CCL2/JE/MCP-1 (Catalog # 279-MC), Recombinant Rat CCL2/JE/MCP 3144-JE), Recombinant Mouse CCL11/Eotaxin (Catalog # 420-ME), Recombinant Mouse CCL12/MCP-5 (Catalog # 428-P5), and Recombinant Mouse CCL7/MCP-3/MARC (456-MC). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse CCL2/JE/MCP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-479-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for CCL2/JE/ MCP-1 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_lightbox">

Western blot shows 25 ng of Recombinant Mouse CCL2/JE/MCP-1 (<a class=

479-JE), Recombinant Human CCL2/JE/MCP-1 (Catalog # 279-MC), Recombinant Rat CCL2/JE/MCP 3144-JE), Recombinant Mouse CCL11/Eotaxin (Catalog # 420-ME), Recombinant Mouse CCL12/MCP-5 (Catalog # 428-P5), and Recombinant Mouse CCL7/MCP-3/MARC (456-MC). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse CCL2/JE/MCP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-479-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for CCL2/JE/ MCP-1 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." alt="Western blot shows 25 ng of Recombinant Mouse CCL2/JE/MCP-1 (479-JE), Recombinant Human CCL2/JE/MCP-1 (Catalog # 279-MC), Recombinant Rat CCL2/JE/MCP 3144-JE), Recombinant Mouse CCL11/Eotaxin (Catalog # 420-ME), Recombinant Mouse CCL12/MCP-5 (Catalog # 428-P5), and Recombinant Mouse CCL7/MCP-3/MARC (456-MC). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse CCL2/JE/MCP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-479-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for CCL2/JE/ MCP-1 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_thumb" />

479-JE) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL2/JE (0.04 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL2/JE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-479-NA). The ND₅₀ is typically 0.5-2.5 µg/mL." class="big_lightbox">

Recombinant Mouse CCL2/JE (Catalog # <a class=

479-JE) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL2/JE (0.04 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL2/JE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-479-NA). The ND₅₀ is typically 0.5-2.5 µg/mL." class="big_thumb" />

479-JE) was serially diluted and captured by Goat Anti-Mouse CCL2/JE/MCP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-479-NA) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CCL2/JE/MCP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-479-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

Recombinant Mouse CCL2/JE/MCP‑1 (Catalog # <a class=

479-JE) was serially diluted and captured by Goat Anti-Mouse CCL2/JE/MCP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-479-NA) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CCL2/JE/MCP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-479-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

479-JE) was serially diluted and captured by Goat Anti-Mouse CCL2/JE/MCP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-479-NA) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CCL2/JE/MCP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-479-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

479-JE) was serially diluted and captured by Goat Anti-Mouse CCL2/JE/MCP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-479-NA) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CCL2/JE/MCP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-479-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF-479-NAU-100UG
Alexa Fluor 405	AF-479-NAV-100UG
Alexa Fluor 488	AF-479-NAG-100UG
Alexa Fluor 532	AF-479-NAX-100UG
Alexa Fluor 594	AF-479-NAT-100UG
Alexa Fluor 647	AF-479-NAR-100UG
Alexa Fluor 700	AF-479-NAN-100UG
Alexa Fluor 750	AF-479-NAS-100UG
Alexa Fluor Plus 405	AF-479-NAAFP405
Alexa Fluor Plus 488	AF-479-NAAFP488
Alexa Fluor Plus 555	AF-479-NAAFP555
Alexa Fluor Plus 594	AF-479-NAAFP594
Alexa Fluor Plus 647	AF-479-NAAFP647
Alexa Fluor Plus 680	AF-479-NAAFP680
Allophycocyanin	AF-479-NAA
Biotin	BAF479
CoraFluor 1	AF-479-NACL1
DyLight 350	AF-479-NAD
DyLight 405	AF-479-NAE
DyLight 488	AF-479-NAK
DyLight 550	AF-479-NAL
DyLight 594	AF-479-NAM
DyLight 650	AF-479-NAW
DyLight 680	AF-479-NAY
DyLight 755	AF-479-NAZ
FITC	AF-479-NAF
HRP	AF-479-NAH
Janelia Fluor 525	AF-479-NAJF525
Janelia Fluor 549	AF-479-NAI
Janelia Fluor 646	AF-479-NAJ
Janelia Fluor 669	AF-479-NAJF669
PE	AF-479-NAP
PerCP	AF-479-NAC
mFluor Violet 450 SE	AF-479-NAMFV450
mFluor Violet 500 SE	AF-479-NAMFV500
mFluor Violet 610 SE	AF-479-NAMFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF-479-SP

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MAB679

CCL2/JE/MCP-1 Antibody (23007) [Unconjugated]

(52 Publications)

100 μg

MAB679

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes and cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

CCL2/JE/MCP-1 was detected in immersion fixed paraffin-embedded sections of human Crohn's disease intestine using Mouse Anti-Human CCL2/JE/MCP-1 Monoclonal Antibody (Catalog # MAB679) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (<a class=

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes and cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="CCL2/JE/MCP-1 was detected in immersion fixed paraffin-embedded sections of human Crohn's disease intestine using Mouse Anti-Human CCL2/JE/MCP-1 Monoclonal Antibody (Catalog # MAB679) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes and cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

279-MC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (AR002). Chemotaxis elicited by Recombinant Human CCL2/MCP-1 (75 ng/mL) is neutralized (green line) by increasing concentrations of Human CCL2/MCP-1 Monoclonal Antibody (Catalog # MAB679). The ND₅₀ is typically 0.5-2.0 µg/mL." class="big_lightbox">

279-MC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (AR002). Chemotaxis elicited by Recombinant Human CCL2/MCP-1 (75 ng/mL) is neutralized (green line) by increasing concentrations of Human CCL2/MCP-1 Monoclonal Antibody (Catalog # MAB679). The ND₅₀ is typically 0.5-2.0 µg/mL." class="big_thumb" />

Expression patterns of CCL2, CCL5, TNF alpha and IL-1 beta in healthy individuals and breast cancer patients. Representative examples of the expression of CCL2, CCL5, TNF alpha and IL-1 beta in the different groups of patients included in the study, in biopsies obtained at the time of diagnosis. (a1-a4) Patients diagnosed with benign breast disorders. The pictures demonstrate the lack of staining of the four factors in the normal breast epithelial cells, as denoted in the majority of patients included in this group. (b1-b4) DCIS patients. The pictures demonstrate positive staining of the four factors in the malignant lesions, as denoted in the majority of patients included in this group. (c1-c4) IDC-no-relapse patients. The pictures demonstrate positive staining of the four factors in the tumor cells, as denoted in the majority of patients included in this group. (d1-d4) IDC-with-relapse patients. The pictures demonstrate positive staining of the four factors in the tumor cells, as denoted in the majority of patients included in this group. (a1, b1, c1, d1) CCL2 staining; (a2, b2, c2, d2) CCL5 staining; (a3, b3, c3, d3) TNF alpha staining; (a4, b4, c4, d4) IL-1 beta staining. The expression of the proteins was determined by IHC using specific antibodies, whose specificity in IHC was verified. The values of photo magnification are indicated in the left bottom corner of each of the pictures. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/21486440), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>Induction of CCL2 and CLXL8 in TNF alpha -stimulated MSCs is not mediated via the AP-1 pathway. (A) Human BM-derived MSCs were stimulated by TNF-alpha (50 ng/ml) for 5 and 10 minutes. Control cells were treated by the vehicle of TNF-alpha . c-Jun levels and phosphorylation were determined by western blot (WB) analyses. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as loading control. (B) Human BM-derived MSCs were transiently transfected by small interfering RNA (siRNA) to c-Jun or by control siRNA. (B1) c-Jun expression was determined by WB analyses. beta -Tubulin was used as loading control. (B2) Following siRNA transfection, the cells were stimulated by TNF-alpha (25 ng/ml; in this part of the study we used a suboptimal concentration of TNF-alpha in order to facilitate detection of inhibitory effects) for 24 hours. Expression levels of CCL2 and CXCL8 in the supernatants of the cells were determined by ELISA, in the linear range of absorbance. #siRNA to c-Jun has yielded minor increases or reductions in CCL2 and CXCL8 secretion in different experiments (see Results and discussion), and thus overall there was no significant effect on CCL2 and CXCL8 secretion. In all panels, the findings are representatives of n = 3 independent experiments that have shown similar results. Image collected and cropped by CiteAb from the following publication (//stemcellres.biomedcentral.com/articles/10.1186/s13287-015-0080-7), licensed under a CC-BY license. Not internally tested by R&D Systems.

NF-kappa B is essential in mediating TNF-alpha -induced release of chemokines by MSCs. (A) Human BM-derived MSCs were stimulated by TNF-alpha (50 ng/ml) for 15 minutes. The levels of I kappa B alpha (the negative regulator of the NF-kappa B pathway) were determined by WB analyses. GAPDH was used as a loading control throughout. (B) CAFs were generated by culturing MSCs with Tumor CM from MDA-MB-231 (MDA) or MCF-7 breast tumor cells over a prolonged period of time (~30 days). TNF-alpha (50 ng/ml) was added for the last 24 hours to MSCs + Tumor CM cells and I kappa B alpha levels were determined by WB analyses. (C) CAF #1 cells were stimulated for 48 hours by TNF-alpha (50 ng/ml). I kappa B alpha levels were determined by WB analyses. (D) Human BM-derived MSCs were stimulated with TNF-alpha (50 ng/ml) for 10 minutes. p65 phosphorylation was determined by WB analyses. (E) Human BM-derived MSCs were transiently transfected by siRNA to p65 or by control siRNA. (E1) p65 expression was determined by WB analyses. (E2) Following siRNA transfection, the cells were stimulated by TNF-alpha (25 ng/ml; a suboptimal concentration of TNF-alpha in order to facilitate detection of inhibitory effects) for 48 hours. Expression of CCL2 and CXCL8 in the supernatants of the cells was determined by ELISA, in the linear range of absorbance. In all panels, the findings are representatives of n = 3 independent experiments that have shown similar results. Image collected and cropped by CiteAb from the following publication (//stemcellres.biomedcentral.com/articles/10.1186/s13287-015-0080-7), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>Microparticle composition influences inflammatory cytokine production from activated monocytes.THP-1 cells (A) or CD14 sorted blood monocytes (B) were treated with control microparticles (grey bars) or PPAR gamma -overexpressing microparticles (black bars) or no microparticles (white bars) for 4 hours before activation with LPS or PAM3CSK4 for 24 hours. Supernatants were collected and pro-inflammatory cytokines IL-8 (top), MCP-1 (bottom) were measured by ELISA. Dotted line represents baseline cytokine production from unactivated cells with no microparticle exposure. Mean values with standard errors represent one of at least 3 experiments. Two-way ANOVA with Tukey's multiple comparison post test was performed to determine statistical significance. * indicates (p<0.05). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25426628), licensed under a CC-BY license. Not internally tested by R&D Systems.

MAB679) at 20ug/mL with 1 hour incubation at room temperature followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and membrane. " class="big_lightbox">

Formalin-fixed paraffin-embedded tissue sections of human Crohn’s Disease were probed for CCL2 mRNA (ACD RNAScope Probe, catalog # 423811; Fast Red chromogen, ACD catalog # 322360). Adjacent tissue section was processed for immunohistochemistry using mouse anti-human CCL2 monoclonal antibody (R&D Systems <a class=

MAB679) at 20ug/mL with 1 hour incubation at room temperature followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and membrane. " class="big_thumb" />

279-MC) was serially diluted and captured by Mouse Anti-Human CCL2/JE/MCP‑1 Monoclonal Antibody (Catalog # MAB679) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CCL2/JE/MCP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-279-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

Recombinant Human CCL2/JE/MCP‑1 (Catalog # <a class=

279-MC) was serially diluted and captured by Mouse Anti-Human CCL2/JE/MCP‑1 Monoclonal Antibody (Catalog # MAB679) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CCL2/JE/MCP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-279-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB679U-100UG
Alexa Fluor 405	FAB679V-100UG
Alexa Fluor 488	FAB679G-100UG
Alexa Fluor 532	FAB679X-100UG
Alexa Fluor 594	FAB679T-100UG
Alexa Fluor 647	FAB679R-100UG
Alexa Fluor 700	FAB679N-100UG
Alexa Fluor 750	FAB679S-100UG
Alexa Fluor Plus 405	FAB679AFP405-100UG
Alexa Fluor Plus 488	FAB679AFP488-100UG
Alexa Fluor Plus 555	FAB679AFP555-100UG
Alexa Fluor Plus 594	FAB679AFP594-100UG
Alexa Fluor Plus 647	FAB679AFP647-100UG
Alexa Fluor Plus 680	FAB679AFP680-100UG
Allophycocyanin	FAB679A
Biotin	FAB679B
CoraFluor 1	FAB679CL1
DyLight 350	FAB679D
DyLight 405	FAB679E
DyLight 488	FAB679K
DyLight 550	FAB679L
DyLight 594	FAB679M
DyLight 650	FAB679W
DyLight 680	FAB679Y
DyLight 755	FAB679Z
FITC	FAB679F
HRP	FAB679H
Janelia Fluor 525	FAB679JF525
Janelia Fluor 549	FAB679I
Janelia Fluor 646	FAB679J
Janelia Fluor 669	FAB679JF669
PE	FAB679P
PerCP	FAB679C
mFluor Violet 450 SE	FAB679MFV450
mFluor Violet 500 SE	FAB679MFV500
mFluor Violet 610 SE	FAB679MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB679-SP
Carrier Free	MAB679-500

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NBP2-22115

CCL2/MCP1 Antibody (2D8) - BSA Free

(1 Review)

(29 Publications)

0.1 ml

NBP2-22115

CCL2-MCP1-Antibody-2D8-BSA-Free-Immunohistochemistry-NBP2-22115-img0009.jpg

Flow Cytometry: CCL2/MCP1 Antibody (2D8) - BSA Free [NBP2-22115] - Analysis of A549 cells using MCP1 mouse mAb (green) and negative control (red).

Western Blot: CCL2/MCP1 Antibody (2D8) - BSA Free [NBP2-22115] - Analysis using MCP1 mAb against human MCP1 (AA: 1-99) recombinant protein. (Expected MW is 36.5 kDa)

<b>Biological Strategies Validation. </b>CCL2-MCP1-Antibody-2D8-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-22115-img0010.jpg

<b>Biological Strategies Validation. </b>CCL2-MCP1-Antibody-2D8-BSA-Free-Immunohistochemistry-NBP2-22115-img0012.jpg

Immunohistochemistry-Paraffin: CCL2/MCP1 Antibody (2D8) - BSA Free [NBP2-22115] - Analysis of paraffin-embedded liver cancer tissues using MCP1 mouse mAb with DAB staining.

ELISA: CCL2/MCP1 Antibody (2D8) - BSA Free [NBP2-22115] - Red: Control Antigen (100ng), Purple: Antigen (10ng), Green: Antigen (50ng), Blue: Antigen (100ng).

Immunocytochemistry/ Immunofluorescence: CCL2/MCP1 Antibody (2D8) - BSA Free [NBP2-22115] - Expression patterns of CCL2 & CCR2 in unwounded & wounded corneas of WT & MMP12 KO mice. (A) Immunofluorescence of CCL2 chemokine & (B) its receptor CCR2 in unwounded & chemically wounded (2-days after injury) WT & Mmp12−/− mouse corneas. Control images represent mouse corneas stained with secondary antibody only & without primary antibody. Nuclei were visualized by staining with DAPI (blue). Scale bars: 50 µm. A magnified image of a wounded WT cornea shows perinuclear expression of CCL2 (orange box). CCL2 staining was visualized in epithelial, stromal, & endothelial layers of wounded WT & Mmp12−/− corneas. CCR2 staining was visualized in epithelial & stromal layers of unwounded & wounded WT & Mmp12−/− corneas. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31399604), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: CCL2/MCP1 Antibody (2D8) - BSA Free [NBP2-22115] - LIF represses CXCL9 through epigenetic silencing. a, b qRT-PCR analysis of the indicated genes in BMDMs. BMDMs were pre-incubated with 20 ng/ml LIF for 72 h & then stimulated with 5 ng/ml IFN gamma or 10 μg/ml IL4 during 6 h (a) or with 0.1, 0.5, 1 & 5 ng/ml IFN gamma for 24 h (b). c CXCL9 ELISA from BMDMs pre-incubated with 20 ng/ml LIF & then stimulated with 0.1 ng/ml IFN gamma for 24 h. d CXCL9 ELISA from human CD11b+sorted cells (77% CD11b+ CD14+, see Supplementary Fig. 7b, c) from human GBM cultured with 20 ng/ml LIF for 72 h & then with 0.1 ng/ml IFN gamma for 24 h. e ChIP of Tri-methyl-histone H3 (H3K27me3), EZH2 & acetyl-histone4 (H4ac) was performed in BMDMs treated with 20 ng/ml LIF for 72 h. Scheme shows the analysed CXCL9 promoter region. Representative data are presented as mean ± SD. f Representative images of IF of the indicated markers in human GBM organotypic slices (patients 1, 2, 3) incubated with 10 μg/ml anti-LIF for 3 days. Scale bar, 20 μm. (see Supplementary Fig. 8). Bottom, percentage of double positive cells relative to Iba1+ cells & percentage of CXCL9+ cells relative to the total number of cells. Data are mean of all patients ± SEM. Statistical analyses by Student’s t-test or Mann–Whitney T-test. *P < 0.05, **P < 0.01; ***P < 0.001; ****P < 0.0001 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31186412), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: CCL2/MCP1 Antibody (2D8) - BSA Free [NBP2-22115] - LIF regulates CXCL9, CCL2, CD206, & CD163 in TAMs. a Differential expression analysis of isolated CD11b+ cells from anti-LIF treated ID8 mice vs. control. Volcano plot representing the genes significantly (Q-value < 0.1) overexpressed (brown) & significantly underexpressed (turquoise). Heatmap representing the expression values of the indicated genes, each column represents a sample & each row a gene. The last column represents the log2 fold change (log2 FC) of gene expression. b mRNA expression for the indicated genes in isolated CD11b+ cells from anti-LIF treated or untreated ID8 & GL261N tumors. c Percentage & mean fluorescence intensity (MFI) of CCL2 & CXCL9 in TAMs (CD11b+ Ly6G− Ly6C−) from anti-LIF treated or untreated GL261N tumors. d Representative IF images of Iba1 & the indicated markers stainings of GL261N tumors (see Supplementary Fig. 4). Scale bar, 20 μm. Bottom, percentage of double positive cells relative to the TAM marker positive cells. CXCL9 quantification is relative to the total number of cells. e Tumor growth of GL261N in WT, CXCL9−/−, & CCL2−/− mice or mice treated with the indicated antibodies is shown as total flux (p/s). f Fold change (FC) of tumor infiltrating CD8+ T cells in the indicated treatments. Data are mean ± SEM. g, h Representative IHC of the indicated markers from 20 GBM tumors. The degree of staining was quantified using H-score method. Correlations between LIF & CCL2, CD206, CD163, & CXCL9 with the R-squared coefficients (R2) were calculated (h). Statistical analyses by Mann–Whitney T-test. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31186412), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: CCL2/MCP1 Antibody (2D8) - BSA Free [NBP2-22115] - LIF regulates CXCL9, CCL2, CD206, & CD163 in TAMs. a Differential expression analysis of isolated CD11b+ cells from anti-LIF treated ID8 mice vs. control. Volcano plot representing the genes significantly (Q-value < 0.1) overexpressed (brown) & significantly underexpressed (turquoise). Heatmap representing the expression values of the indicated genes, each column represents a sample & each row a gene. The last column represents the log2 fold change (log2 FC) of gene expression. b mRNA expression for the indicated genes in isolated CD11b+ cells from anti-LIF treated or untreated ID8 & GL261N tumors. c Percentage & mean fluorescence intensity (MFI) of CCL2 & CXCL9 in TAMs (CD11b+ Ly6G− Ly6C−) from anti-LIF treated or untreated GL261N tumors. d Representative IF images of Iba1 & the indicated markers stainings of GL261N tumors (see Supplementary Fig. 4). Scale bar, 20 μm. Bottom, percentage of double positive cells relative to the TAM marker positive cells. CXCL9 quantification is relative to the total number of cells. e Tumor growth of GL261N in WT, CXCL9−/−, & CCL2−/− mice or mice treated with the indicated antibodies is shown as total flux (p/s). f Fold change (FC) of tumor infiltrating CD8+ T cells in the indicated treatments. Data are mean ± SEM. g, h Representative IHC of the indicated markers from 20 GBM tumors. The degree of staining was quantified using H-score method. Correlations between LIF & CCL2, CD206, CD163, & CXCL9 with the R-squared coefficients (R2) were calculated (h). Statistical analyses by Mann–Whitney T-test. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31186412), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

The production of MCP-1, VCAM-1, and ICAM-1 was significantly inhibited in Kaempferol treatment during sepsis(A) The protein expression level of MCP-1, VCAM-1, and ICAM-1 at 24 h; (B) protein expression level of MCP-1, VCAM-1, and ICAM-1 at 48 h in sham, saline-treated CLP mice, and kaempferol treated CLP mice (n = 3 per group, mean +/- S.E.M, *P<0.05, **P<0.01, ***P<0.001). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37440431), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Fe, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Fe, Pm

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-22115AF350
Alexa Fluor 405	NBP2-22115AF405
Alexa Fluor 488	NBP2-22115AF488
Alexa Fluor 532	NBP2-22115AF532
Alexa Fluor 594	NBP2-22115AF594
Alexa Fluor 647	NBP2-22115AF647
Alexa Fluor 700	NBP2-22115AF700
Alexa Fluor 750	NBP2-22115AF750
Alexa Fluor Plus 405	NBP2-22115AFP405
Alexa Fluor Plus 488	NBP2-22115AFP488
Alexa Fluor Plus 555	NBP2-22115AFP555
Alexa Fluor Plus 594	NBP2-22115AFP594
Alexa Fluor Plus 647	NBP2-22115AFP647
Alexa Fluor Plus 680	NBP2-22115AFP680
Allophycocyanin	NBP2-22115APC
Biotin	NBP2-22115B
CoraFluor 1	NBP2-22115CL1
DyLight 350	NBP2-22115UV
DyLight 405	NBP2-22115V
DyLight 488	NBP2-22115G
DyLight 550	NBP2-22115R
DyLight 594	NBP2-22115DL594
DyLight 650	NBP2-22115C
DyLight 680	NBP2-22115FR
DyLight 755	NBP2-22115IR
FITC	NBP2-22115F
HRP	NBP2-22115H
Janelia Fluor 525	NBP2-22115JF525
Janelia Fluor 549	NBP2-22115JF549
Janelia Fluor 646	NBP2-22115JF646
Janelia Fluor 669	NBP2-22115JF669
PE	NBP2-22115PE
PerCP	NBP2-22115PCP
mFluor Violet 450 SE	NBP2-22115MFV450
mFluor Violet 500 SE	NBP2-22115MFV500
mFluor Violet 610 SE	NBP2-22115MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-22115SS
Azide and BSA Free	NBP2-80618

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NBP1-07035

CCL2/MCP1 Antibody - BSA Free

(1 Review)

(26 Publications)

0.1 ml

NBP1-07035

<b>Biological Strategies Validation. </b>CCL2-MCP1-Antibody-Western-Blot-NBP1-07035-img0013.jpg

Immunocytochemistry/Immunofluorescence: CCL2/MCP1 Antibody [NBP1-07035] - MCP1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Immunohistochemistry-Paraffin: CCL2/MCP1 Antibody [NBP1-07035] - IHC analysis of a formalin-fixed paraffin-embedded (FFPE) human breast carcinoma tissue section using 1:1000 dilution of CCL2/MCP1 antibody (NBP1-07035) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This antibody generated a diffused cytoplasmic staining of CCL2 antigen in the cancer cells, stromal cells as well as the endothelial cells. The stroma itself showed a weak immunopositivity for CCL2. Staining was performed by Histowiz.

Western Blot: CCL2/MCP1 Antibody [NBP1-07035] - CCL2 expression level was evaluated in control group and aLAG-3 group.

Western Blot: CCL2/MCP1 Antibody - BSA Free [NBP1-07035] - Ox-LDL induced macrophage foam cell formation, SIRT1 inhibition, autophagy impairment, & MCP-1 production in THP-1 cellsHuman THP-1 macrophages were exposed to 0, 20, 40, 60, & 80 μg/mL of ox-LDL for 24 hrs. Treated cells were photographed using light microscopy (A). The THP-1 macrophage-derived foam cell formation was determined using ORO staining method (B) & (C). Western blot for SIRT1, LC3, Beclin1, p62, & MCP-1 proteins were analyzed from the ox-LDL-stimulated THP-1 cells. beta -actin was used as loading control (D-I). Scale bar: 20 μm. Bar graph indicates the mean ± SD (n = 3). *P < 0.05 & **P < 0.01 vs. Cont group (0 μg/mL of ox-LDL). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.17691), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: CCL2/MCP1 Antibody - BSA Free [NBP1-07035] - Inhibition of autophagy with 3-MA led to increased foam cell formation & MCP-1 productionHuman THP-1 macrophages were pretreated with 3-MA (5 μM) for 2 hrs, & then exposed to 80 μg/mL of ox-LDL for an additional 24 hrs. Western blot for LC3, Beclin1, p62, & MCP-1 proteins were analyzed from the ox-LDL-stimulated THP-1 cells. beta -actin was used as loading control (A-E). THP-1 macrophage-derived foam cell formation was determined using ORO staining method (F-G). Scale bar: 40 μm. Bar graph indicates the mean ± SD (n = 3). *P < 0.05 & **P < 0.01 vs. Cont group; #P < 0.05 & ##P < 0.01 vs. 3-MA group; &P < 0.05 & &&P < 0.01 represent significant differences between ox-LDL group & 3-MA+ox-LDL group. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.17691), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: CCL2/MCP1 Antibody - BSA Free [NBP1-07035] - CCL2-ir cellular distribution in ependymal layers attached to the CC. A, B CCL2-ir (CCL2 panels, arrows) & GFAP (GFAP panels, arrowheads) double immunostained structures (Merged panels, arrow-arrowheads) are seen inlaid in the ependymal layers attached to the CC in both saline & LPS injected mice. C A CCL2-ir & Iba-1 double labeled soma (Merged, arrow-arrowhead) is seen at a corner of lateral ventricle, which seems be just next to an invaginated choroid plexus between the CC & basal ganglion (framed areas & insets). D, E Hyper-ramified & amoeba like Iba-1 labeled cells (Iba-1 panel, opened arrowheads) are observed in the CC of LPS injected mice, indicating their activated states. The CCL2-ir & Iba-1 double labeled structures are also regarded in the ependymal layers (Merged, arrow-arrowheads). Scare bar = 100 µm in all A– E Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35354428), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: CCL2/MCP1 Antibody - BSA Free [NBP1-07035] - Inhibition of autophagy using Atg5 siRNA aggravated foam cell formation & MCP-1 expressionHuman THP-1 macrophages were pretreated with Atg5 siRNA (20 μM) for 24 hrs, & then exposed to 80 μg/mL of ox-LDL for an additional 24 hrs. Western blot forAtg5, LC3, Beclin1, p62, & MCP-1 proteins were analyzed from the ox-LDL-stimulated THP-1 cells. beta -actin was used as loading control (A-F) & (I). THP-1 macrophage-derived foam cell formation was determined using ORO staining method (G) & (H). Scale bar: 40 μm. Bar graph indicates the mean ± SD (n = 3). *P < 0.05 & **P < 0.01 vs. NC siRNA group; #P < 0.05 & ##P < 0.01 vs. Atg5 siRNA group; &P < 0.05 & &&P < 0.01 represent significant differences between NC siRNA+ox-LDL group & Atg5 siRNA+ox-LDL group. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.17691), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: CCL2/MCP1 Antibody - BSA Free [NBP1-07035] - Inhibition of SIRT1 using EX-527 or SIRT1 siRNA transfection enhanced MCP-1 expression & foam cell formationHuman THP-1 macrophages were pretreated with EX-527 (2 μM, for 2 hrs) or SIRT1 siRNA (20 μM, for 24 hrs), & then exposed to 80 μg/mL of ox-LDL for an additional 24 hrs. Western blot for SIRT1 & MCP-1 proteins were analyzed from the ox-LDL-stimulated THP-1 cells. beta -actin was used as loading control (A-C) & (F-H). THP-1 macrophage-derived foam cell formation was determined using ORO staining method (D-E) & (I-J). Scale bar: 40 μm. Bar graph indicates the mean ± SD (n = 3). *P < 0.05 & **P < 0.01 vs. Cont group (NC siRNA group); #P < 0.05 & ##P < 0.01 vs. EX-527 group (SIRT1 siRNA group); &P < 0.05 & &&P < 0.01 represent significant differences between ox-LDL group (NC siRNA+ox-LDL group) & EX-527+ox-LDL group (SIRT1 siRNA+ox-LDL group). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.17691), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: CCL2/MCP1 Antibody - BSA Free [NBP1-07035] - Overexpression of SIRT1 using adenoviral transfection reversed ox-LDL-induced macrophage foam cell formation & autophagy impairment in THP-1 cellsHuman THP-1 macrophages were transfected by SIRT1 over-expressing adenovirus (HBAD-SIRT1) or NC adenovirus (HBAD-GFP) for 24 hrs & then exposed to 80 μg/mL of ox-LDL for an additional 24 hrs. The transfected THP-1 cells were observed using an inverted fluorescence microscope (A) & then were harvested for transfection efficiency analysis by Western blot method (B). THP-1 macrophage-derived foam cell formation was determined using ORO staining method (C) & (D). Western blot for LC3, Beclin1, p62, & Atg5 proteins & immunoprecipitation for acetyl-Lys Atg5 were analyzed from the ox-LDL-stimulated THP-1 cells. beta -actin was used as loading control (E-L). Scale bar: 40 μm. Bar graph indicates the mean ± SD (n = 3). *P < 0.05 & **P < 0.01 vs. HBAD-GFP group; #P < 0.05 & ##P < 0.01 vs. HBAD-SIRT1 group; &P < 0.05 & &&P < 0.01 represent significant differences between HBAD-GFP+ox-LDL group & HBAD-SIRT1+ox-LDL group. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.17691), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-07035AF350
Alexa Fluor 405	NBP1-07035AF405
Alexa Fluor 488	NBP1-07035AF488
Alexa Fluor 532	NBP1-07035AF532
Alexa Fluor 594	NBP1-07035AF594
Alexa Fluor 647	NBP1-07035AF647
Alexa Fluor 700	NBP1-07035AF700
Alexa Fluor 750	NBP1-07035AF750
Alexa Fluor Plus 405	NBP1-07035AFP405
Alexa Fluor Plus 488	NBP1-07035AFP488
Alexa Fluor Plus 555	NBP1-07035AFP555
Alexa Fluor Plus 594	NBP1-07035AFP594
Alexa Fluor Plus 647	NBP1-07035AFP647
Alexa Fluor Plus 680	NBP1-07035AFP680
Allophycocyanin	NBP1-07035APC
Biotin	NBP1-07035B
CoraFluor 1	NBP1-07035CL1
DyLight 350	NBP1-07035UV
DyLight 405	NBP1-07035V
DyLight 488	NBP1-07035G
DyLight 550	NBP1-07035R
DyLight 594	NBP1-07035DL594
DyLight 650	NBP1-07035C
DyLight 680	NBP1-07035FR
DyLight 755	NBP1-07035IR
FITC	NBP1-07035F
HRP	NBP1-07035H
Janelia Fluor 525	NBP1-07035JF525
Janelia Fluor 549	NBP1-07035JF549
Janelia Fluor 646	NBP1-07035JF646
Janelia Fluor 669	NBP1-07035JF669
PE	NBP1-07035PE
PerCP	NBP1-07035PCP
mFluor Violet 450 SE	NBP1-07035MFV450
mFluor Violet 500 SE	NBP1-07035MFV500
mFluor Violet 610 SE	NBP1-07035MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-07035SS

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MAB279

CCL2/JE/MCP-1 Antibody (24822) [Unconjugated]

(29 Publications)

100 μg

MAB279

279-MC), Recombinant Human CCL8/ MCP-2 (Catalog # 281-CP), Recombinant Human CCL7/MCP-3/MARC (Catalog # 282-P3), Recombinant Human CCL13/ MCP-4 (Catalog # 327-P4), Recombinant Human CCL11/Eotaxin (Catalog # 320-EO), Recombinant Mouse CCL2/JE/MCP-1 (Catalog # 479-JE), and Recombinant Rat CCL2/JE/MCP-1 (Catalog # 3144-JE). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human CCL2/JE/MCP-1 Monoclonal Antibody (Catalog # MAB279) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CCL2/JE/ MCP-1 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_lightbox">

Western blot shows 25 ng of Recombinant Human CCL2/JE/MCP-1 (Catalog # <a class=

279-MC), Recombinant Human CCL8/ MCP-2 (Catalog # 281-CP), Recombinant Human CCL7/MCP-3/MARC (Catalog # 282-P3), Recombinant Human CCL13/ MCP-4 (Catalog # 327-P4), Recombinant Human CCL11/Eotaxin (Catalog # 320-EO), Recombinant Mouse CCL2/JE/MCP-1 (Catalog # 479-JE), and Recombinant Rat CCL2/JE/MCP-1 (Catalog # 3144-JE). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human CCL2/JE/MCP-1 Monoclonal Antibody (Catalog # MAB279) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CCL2/JE/ MCP-1 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." alt="Western blot shows 25 ng of Recombinant Human CCL2/JE/MCP-1 (Catalog # 279-MC), Recombinant Human CCL8/ MCP-2 (Catalog # 281-CP), Recombinant Human CCL7/MCP-3/MARC (Catalog # 282-P3), Recombinant Human CCL13/ MCP-4 (Catalog # 327-P4), Recombinant Human CCL11/Eotaxin (Catalog # 320-EO), Recombinant Mouse CCL2/JE/MCP-1 (Catalog # 479-JE), and Recombinant Rat CCL2/JE/MCP-1 (Catalog # 3144-JE). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human CCL2/JE/MCP-1 Monoclonal Antibody (Catalog # MAB279) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CCL2/JE/ MCP-1 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_thumb" />

279-MC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL2/JE/ MCP-1 (0.15 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL2/JE/ MCP-1 Monoclonal Antibody (Catalog # MAB279). The ND₅₀ is typically 0.9-4.5 µg/mL." class="big_lightbox">

279-MC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL2/JE/ MCP-1 (0.15 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL2/JE/ MCP-1 Monoclonal Antibody (Catalog # MAB279). The ND₅₀ is typically 0.9-4.5 µg/mL." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB279U
Alexa Fluor 405	FAB279V
Alexa Fluor 532	FAB279X
Alexa Fluor 594	FAB279T
Alexa Fluor 647	FAB279R
Alexa Fluor 700	FAB279N
Alexa Fluor 750	FAB279S
Allophycocyanin	FAB279A
Biotin	FAB279B
CoraFluor 1	FAB279CL1
DyLight 350	FAB279D
DyLight 405	FAB279E
DyLight 488	FAB279K
DyLight 550	FAB279L
DyLight 594	FAB279M
DyLight 650	FAB279W
DyLight 680	FAB279Y
DyLight 755	FAB279Z
FITC	FAB279F
HRP	FAB279H
Janelia Fluor 525	FAB279JF525
Janelia Fluor 549	FAB279I
Janelia Fluor 646	FAB279J
Janelia Fluor 669	FAB279JF669
PE	FAB279P
PerCP	FAB279C
mFluor Violet 450 SE	FAB279MFV450
mFluor Violet 500 SE	FAB279MFV500
mFluor Violet 610 SE	FAB279MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB279-SP
Carrier Free	MAB279-500

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AF-279-NA

CCL2/JE/MCP-1 Antibody [Unconjugated]

(21 Publications)

100 μg

AF-279-NA

279-MC), Recombinant Mouse CCL2/JE/MCP-1 (Catalog # 479-JE), Recombinant Rat CCL2/JE/MCP-1 (Catalog # 3144-JE), Recombinant Human CCL7/MCP-3/MARC (Catalog # 282-P3), Recombinant Human CCL11/Eotaxin (Catalog # 320-EO), Recombinant Human CCL8/MCP-2 (Catalog # 281-CP), and Recombinant Human CCL13/MCP-4 (Catalog # 327-P4). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human CCL2/JE/MCP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-279-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CCL2/JE/MCP-1 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_lightbox">

279-MC), Recombinant Mouse CCL2/JE/MCP-1 (Catalog # 479-JE), Recombinant Rat CCL2/JE/MCP-1 (Catalog # 3144-JE), Recombinant Human CCL7/MCP-3/MARC (Catalog # 282-P3), Recombinant Human CCL11/Eotaxin (Catalog # 320-EO), Recombinant Human CCL8/MCP-2 (Catalog # 281-CP), and Recombinant Human CCL13/MCP-4 (Catalog # 327-P4). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human CCL2/JE/MCP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-279-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CCL2/JE/MCP-1 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." alt="Western blot shows 25 ng of Recombinant Human CCL2/JE/MCP-1 (Catalog # 279-MC), Recombinant Mouse CCL2/JE/MCP-1 (Catalog # 479-JE), Recombinant Rat CCL2/JE/MCP-1 (Catalog # 3144-JE), Recombinant Human CCL7/MCP-3/MARC (Catalog # 282-P3), Recombinant Human CCL11/Eotaxin (Catalog # 320-EO), Recombinant Human CCL8/MCP-2 (Catalog # 281-CP), and Recombinant Human CCL13/MCP-4 (Catalog # 327-P4). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human CCL2/JE/MCP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-279-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CCL2/JE/MCP-1 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_thumb" />

VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

CCL2/JE/MCP-1 was detected in immersion fixed paraffin-embedded sections of human Crohn's disease intestine using Goat Anti-Human CCL2/JE/MCP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-279-NA) at 25 µg/mL for overnight at 4° C temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

279-MC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL2/MCP-1 (150 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL2/MCP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-279-NA). The ND₅₀ is typically 1.5-4.5 µg/mL." class="big_lightbox">

Recombinant Human CCL2/ MCP-1 (Catalog # <a class=

279-MC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL2/MCP-1 (150 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL2/MCP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-279-NA). The ND₅₀ is typically 1.5-4.5 µg/mL." class="big_thumb" />

279-MC) was serially diluted and captured by Mouse Anti-Human CCL2/JE/MCP‑1 Monoclonal Antibody (Catalog # MAB679) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CCL2/JE/MCP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-279-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

279-MC) was serially diluted and captured by Mouse Anti-Human CCL2/JE/MCP‑1 Monoclonal Antibody (Catalog # MAB679) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CCL2/JE/MCP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-279-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF-279-NAU-100UG
Alexa Fluor 405	AF-279-NAV-100UG
Alexa Fluor 488	AF-279-NAG-100UG
Alexa Fluor 532	AF-279-NAX-100UG
Alexa Fluor 594	AF-279-NAT-100UG
Alexa Fluor 647	AF-279-NAR-100UG
Alexa Fluor 700	AF-279-NAN-100UG
Alexa Fluor 750	AF-279-NAS-100UG
Alexa Fluor Plus 405	AF-279-NAAFP405
Alexa Fluor Plus 488	AF-279-NAAFP488
Alexa Fluor Plus 555	AF-279-NAAFP555
Alexa Fluor Plus 594	AF-279-NAAFP594
Alexa Fluor Plus 647	AF-279-NAAFP647
Alexa Fluor Plus 680	AF-279-NAAFP680
Allophycocyanin	AF-279-NAA
Biotin	BAF279
CoraFluor 1	AF-279-NACL1
DyLight 350	AF-279-NAD
DyLight 405	AF-279-NAE
DyLight 488	AF-279-NAK
DyLight 550	AF-279-NAL
DyLight 594	AF-279-NAM
DyLight 650	AF-279-NAW
DyLight 680	AF-279-NAY
DyLight 755	AF-279-NAZ
FITC	AF-279-NAF
HRP	AF-279-NAH
Janelia Fluor 525	AF-279-NAJF525
Janelia Fluor 549	AF-279-NAI
Janelia Fluor 646	AF-279-NAJ
Janelia Fluor 669	AF-279-NAJF669
PE	AF-279-NAP
PerCP	AF-279-NAC
mFluor Violet 450 SE	AF-279-NAMFV450
mFluor Violet 500 SE	AF-279-NAMFV500
mFluor Violet 610 SE	AF-279-NAMFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF-279-SP

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MAB479

CCL2/JE/MCP-1 Antibody (123616)

(25 Publications)

100 μg

MAB479

479-JE) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL2/JE (40 ng/mL) is neutralized (green line) by increasing concentrations of Mouse CCL2/JE Monoclonal Antibody (Catalog # MAB479). The ND₅₀ is typically 0.75-3.0 µg/mL." class="big_lightbox">

479-JE) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL2/JE (40 ng/mL) is neutralized (green line) by increasing concentrations of Mouse CCL2/JE Monoclonal Antibody (Catalog # MAB479). The ND₅₀ is typically 0.75-3.0 µg/mL." alt="Recombinant Mouse CCL2/JE (Catalog # 479-JE) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL2/JE (40 ng/mL) is neutralized (green line) by increasing concentrations of Mouse CCL2/JE Monoclonal Antibody (Catalog # MAB479). The ND₅₀ is typically 0.75-3.0 µg/mL." class="big_thumb" />

Tryptase directly induced the expression of angiogenic factors in retinal endothelial cells.(F) Addition of recombinant mMCP6 into the culture of primary retinal endothelial cells induced typical tube formation. The effect of mMCP6 was suppressed by addition of anti-MCP1 mAbs. n = 4 in each group. *P < 0.05 versus rat isotype mAb, Dunnett’s test. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/28990934), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB479U-100UG
Alexa Fluor 405	FAB479V-100UG
Alexa Fluor 488	FAB479G-100UG
Alexa Fluor 532	FAB479X-100UG
Alexa Fluor 594	FAB479T-100UG
Alexa Fluor 700	FAB479N-100UG
Alexa Fluor 750	FAB479S-100UG
Alexa Fluor Plus 405	FAB479AFP405-100UG
Alexa Fluor Plus 488	FAB479AFP488-100UG
Alexa Fluor Plus 555	FAB479AFP555-100UG
Alexa Fluor Plus 594	FAB479AFP594-100UG
Alexa Fluor Plus 647	FAB479AFP647-100UG
Alexa Fluor Plus 680	FAB479AFP680-100UG
Biotin	FAB479B
CoraFluor 1	FAB479CL1
DyLight 350	FAB479D
DyLight 405	FAB479E
DyLight 488	FAB479K
DyLight 550	FAB479L
DyLight 594	FAB479M
DyLight 650	FAB479W
DyLight 680	FAB479Y
DyLight 755	FAB479Z
FITC	FAB479F
HRP	FAB479H
Janelia Fluor 525	FAB479JF525
Janelia Fluor 549	FAB479I
Janelia Fluor 646	FAB479J
Janelia Fluor 669	FAB479JF669
PE	FAB479P
PerCP	FAB479C
mFluor Violet 450 SE	FAB479MFV450
mFluor Violet 500 SE	FAB479MFV500
mFluor Violet 610 SE	FAB479MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB479-SP
Carrier Free	MAB479-500

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Kit Type

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

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