| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-65599SS | |||
NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="Integrin beta 4/CD104 was detected in immersion fixed A431 human epithelial carcinoma cell line using Mouse Anti-Human Integrin beta 4/CD104 Monoclonal Antibody (Catalog # MAB4060) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | FAB4060G | |||
| Carrier Free | FAB4060A | |||
| Carrier Free | MAB4060-SP | |||
| Carrier Free | FAB4060V-100UG | |||
| Carrier Free | FAB4060U-100UG | |||
| Carrier Free | FAB4060T-100UG | |||
| Carrier Free | FAB4060S-100UG | |||
| Carrier Free | FAB4060R-100UG | |||
| Carrier Free | FAB4060N-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38297-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB4054-SP | |||
| Carrier Free | FAB4054V-100UG | |||
| Carrier Free | FAB4054U-100UG | |||
| Carrier Free | FAB4054T-100UG | |||
| Carrier Free | FAB4054S-100UG | |||
| Carrier Free | FAB4054R-100UG | |||
| Carrier Free | FAB4054N-100UG | |||
| Carrier Free | FAB4054G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF4054-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44420 | |||
| BSA Free | NBP2-76525-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44419 | |||
| BSA Free | NBP2-76524-25ul | |||
NL010) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="Integrin beta 4/CD104 was detected in immersion fixed A431 human epithelial carcinoma cell line using Sheep Anti-Human Integrin beta 4/CD104 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4060) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF4060-SP | |||
![Immunohistochemistry-Frozen: Integrin beta 4/CD104 Antibody (450-9D) [NB100-65599] - Staining of human tonsil cryostat section with Mouse anti human CD104](http://images.novusbio.com/fullsize/Integrin-beta-4-CD104-Antibody-450-9D-Immunohistochemistry-Frozen-NB100-65599-img0002.jpg "Immunohistochemistry-Frozen: Integrin beta 4/CD104 Antibody (450-9D) [NB100-65599] - Staining of human tonsil cryostat section with Mouse anti human CD104")
![Immunocytochemistry/Immunofluorescence: Integrin beta 4/CD104 Antibody [NBP2-38297] - Staining of human cell line HaCaT shows localization to nucleus, plasma membrane & cell junctions. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Integrin-beta-4-CD104-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38297-img0003.jpg "Immunocytochemistry/Immunofluorescence: Integrin beta 4/CD104 Antibody [NBP2-38297] - Staining of human cell line HaCaT shows localization to nucleus, plasma membrane & cell junctions. Antibody staining is shown in green.")
![Flow Cytometry: Integrin beta 4/CD104 Antibody (439-9B) [NBP1-43369] - Analysis using the Biotin conjugate of NBP1-43369. Staining of the A549 cell line with 0.125 ug of Rat IgG2b k Isotype Control Biotin (open histogram) or 0.125 ug of Anti-Human CD104 (Integrin BETA 4) Biotin (filled histogram) followed by Streptavidin PE.](http://images.novusbio.com/fullsize/Integrin-beta-4-CD104-Antibody-439-9B-Flow-Cytometry-NBP1-43369-img0004.jpg "Flow Cytometry: Integrin beta 4/CD104 Antibody (439-9B) [NBP1-43369] - Analysis using the Biotin conjugate of NBP1-43369. Staining of the A549 cell line with 0.125 ug of Rat IgG2b k Isotype Control Biotin (open histogram) or 0.125 ug of Anti-Human CD104 (Integrin BETA 4) Biotin (filled histogram) followed by Streptavidin PE.")
![Flow Cytometry: Integrin beta 4/CD104 Antibody (439-9B) [NBP1-43369] - Staining of the A549 cell line with 0.5 ug of Rat IgG2b k Isotype Control Purified (open histogram) or 0.5 ug of Anti-Human CD104 (Integrin beta 4) Purified (filled histogram) followed by Anti-Rat IgG Biotin and Streptavidin PE. Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/Integrin-beta-4-CD104-Antibody-439-9B-Flow-Cytometry-NBP1-43369-img0002.jpg)
![Western Blot: Integrin beta 4/CD104 Antibody (CL6973) [NBP2-76525] - Analysis in human cell line A-431.](http://images.novusbio.com/fullsize/Integrin-beta-4-CD104-Antibody-CL6973-Western-Blot-NBP2-76525-img0001.jpg "Western Blot: Integrin beta 4/CD104 Antibody (CL6973) [NBP2-76525] - Analysis in human cell line A-431.")
![Immunohistochemistry-Paraffin: Integrin beta 4/CD104 Antibody (CL6973) [NBP2-76525] - Staining of human placenta shows strong positivity in basement membrane of trophoblastic cells.](http://images.novusbio.com/fullsize/Integrin-beta-4-CD104-Antibody-CL6973-Immunohistochemistry-Paraffin-NBP2-76525-img0006.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Integrin beta 4/CD104 Antibody (CL6973) [NBP2-76525] - Analysis in human duodenum and liver tissues. Corresponding ITGB4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Integrin-beta-4-CD104-Antibody-CL6973-Immunohistochemistry-Paraffin-NBP2-76525-img0002.jpg)
![Immunohistochemistry-Paraffin: Integrin beta 4/CD104 Antibody (CL6973) [NBP2-76525] - Staining of human duodenum shows moderate membranous immunoreactivity in glandular cells and strong positivity in basement membrane.](http://images.novusbio.com/fullsize/Integrin-beta-4-CD104-Antibody-CL6973-Immunohistochemistry-Paraffin-NBP2-76525-img0003.jpg)
![Immunohistochemistry-Paraffin: Integrin beta 4/CD104 Antibody (CL6973) [NBP2-76525] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Integrin-beta-4-CD104-Antibody-CL6973-Immunohistochemistry-Paraffin-NBP2-76525-img0005.jpg)
![Immunohistochemistry-Paraffin: Integrin beta 4/CD104 Antibody (CL6973) [NBP2-76525] - Staining of human prostate shows moderate membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Integrin-beta-4-CD104-Antibody-CL6973-Immunohistochemistry-Paraffin-NBP2-76525-img0004.jpg)
![Western Blot: Integrin beta 4/CD104 Antibody (CL6972) [NBP2-76524] - Analysis in human cell line A-431.](http://images.novusbio.com/fullsize/Integrin-beta-4-CD104-Antibody-CL6972-Western-Blot-NBP2-76524-img0001.jpg "Western Blot: Integrin beta 4/CD104 Antibody (CL6972) [NBP2-76524] - Analysis in human cell line A-431.")
![Immunohistochemistry-Paraffin: Integrin beta 4/CD104 Antibody (CL6972) [NBP2-76524] - Staining of human placenta shows moderate to strong positivity in basement membrane of trophoblastic cells.](http://images.novusbio.com/fullsize/Integrin-beta-4-CD104-Antibody-CL6972-Immunohistochemistry-Paraffin-NBP2-76524-img0006.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Integrin beta 4/CD104 Antibody (CL6972) [NBP2-76524] - Analysis in human small intestine and liver tissues. Corresponding ITGB4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Integrin-beta-4-CD104-Antibody-CL6972-Immunohistochemistry-Paraffin-NBP2-76524-img0002.jpg)
![Immunohistochemistry-Paraffin: Integrin beta 4/CD104 Antibody (CL6972) [NBP2-76524] - Staining of human small intestine shows moderate positivity in basement membrane of glandular cells.](http://images.novusbio.com/fullsize/Integrin-beta-4-CD104-Antibody-CL6972-Immunohistochemistry-Paraffin-NBP2-76524-img0003.jpg)
![Immunohistochemistry-Paraffin: Integrin beta 4/CD104 Antibody (CL6972) [NBP2-76524] - Staining of human liver shows no positivity as expected.](http://images.novusbio.com/fullsize/Integrin-beta-4-CD104-Antibody-CL6972-Immunohistochemistry-Paraffin-NBP2-76524-img0005.jpg)
![Immunohistochemistry-Paraffin: Integrin beta 4/CD104 Antibody (CL6972) [NBP2-76524] - Staining of human prostate shows moderate positivity in basement membrane of glandular cells.](http://images.novusbio.com/fullsize/Integrin-beta-4-CD104-Antibody-CL6972-Immunohistochemistry-Paraffin-NBP2-76524-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Integrin beta 4/CD104 [p Tyr1494] Antibody [NBP3-23397] - Immunocytochemical labeling of Integrin beta 4/CD104 in control (Top) and pervanadate-treated A431 cells (Bottom). The cells were labeled with mouse monoclonal anti-Integrin beta 4/CD104 (Cytoplasmic region) (left) or rabbit polyclonals anti-Integrin beta 4/CD104 (Tyr-1494) (middle) or anti-Integrin beta 4/CD104 (Tyr-1526) (right), then the antibodies were detected using appropriate secondary antibodies conjugated to DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23397_rabbit-integrin-beta-4-cd104-p-tyr1494-pab-119202316331611.jpg "<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Integrin beta 4/CD104 [p Tyr1494] Antibody [NBP3-23397] - Immunocytochemical labeling of Integrin beta 4/CD104 in control (Top) and pervanadate-treated A431 cells (Bottom). The cells were labeled with mouse monoclonal anti-Integrin beta 4/CD104 (Cytoplasmic region) (left) or rabbit polyclonals anti-Integrin beta 4/CD104 (Tyr-1494) (middle) or anti-Integrin beta 4/CD104 (Tyr-1526) (right), then the antibodies were detected using appropriate secondary antibodies conjugated to DyLight(R) 594.")
![<b>Biological Strategies Validation. </b>Western Blot: Integrin beta 4/CD104 [p Tyr1494] Antibody [NBP3-23397] - Western blot analysis of A431 cells serum starved overnight (lanes 1, 3, & 5) and treated with pervanadate (1 mM) for 30 min (lanes 2, 4, & 6). The blots were probed with rabbit polyclonal anti-Integrin beta 4/CD104 (Tyr-1526) (lanes 1 & 2) and anti-Integrin beta 4/CD104 (Tyr-1494) (lanes 3 & 4) or with mouse monoclonal anti-Integrin beta 4/CD104 (lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23397_rabbit-integrin-beta-4-cd104-p-tyr1494-pab-1192023166538.jpg)
![Immunocytochemistry/Immunofluorescence: Integrin beta 4/CD104 [p Tyr1526] Antibody [NBP3-23401] - Immunocytochemical labeling of Integrin beta 4/CD104 in control (Top) and pervanadate-treated A431 cells (Bottom). The cells were labeled with mouse monoclonal anti-Integrin beta 4/CD104 (Cytoplasmic region) (left) or rabbit polyclonals anti-Integrin beta 4/CD104 (Tyr-1494) (middle) or anti-Integrin beta 4/CD104 (Tyr-1526) (right), then the antibodies were detected using appropriate secondary antibodies conjugated to DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23401_rabbit-integrin-beta-4-cd104-p-tyr1526-pab-199202312523611.jpg "<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Integrin beta 4/CD104 [p Tyr1526] Antibody [NBP3-23401] - Immunocytochemical labeling of Integrin beta 4/CD104 in control (Top) and pervanadate-treated A431 cells (Bottom). The cells were labeled with mouse monoclonal anti-Integrin beta 4/CD104 (Cytoplasmic region) (left) or rabbit polyclonals anti-Integrin beta 4/CD104 (Tyr-1494) (middle) or anti-Integrin beta 4/CD104 (Tyr-1526) (right), then the antibodies were detected using appropriate secondary antibodies conjugated to DyLight(R) 594.")
![<b>Biological Strategies Validation. </b>Western Blot: Integrin beta 4/CD104 [p Tyr1526] Antibody [NBP3-23401] - Western blot analysis of A431 cells serum starved overnight (lanes 1, 3, & 5) and treated with pervanadate (1 mM) for 30 min (lanes 2, 4, & 6). The blots were probed with rabbit polyclonal anti-Integrin beta 4/CD104 (Tyr-1526) (lanes 1 & 2) and anti-Integrin beta 4/CD104 (Tyr-1494) (lanes 3 & 4) or with mouse monoclonal anti-Integrin beta 4/CD104 (lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23401_rabbit-integrin-beta-4-cd104-p-tyr1526-pab-19920231992822.jpg)
