Products Related to Gene-ID: 23411

HAF016). A specific band was detected for Sirtuin 1/SIRT1 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF016). A specific band was detected for Sirtuin 1/SIRT1 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of A172 human glioblastoma cell line, A549 human lung carcinoma cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human Sirtuin 1/SIRT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7714) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Sirtuin 1/SIRT1 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules." class="big_lightbox"> 5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules." class="big_thumb" />

HAF016). A specific band was detected for Sirtuin 1/SIRT1 at approximately 120 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF016). A specific band was detected for Sirtuin 1/SIRT1 at approximately 120 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL007). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL007). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="Sirtuin 1/SIRT1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Sirtuin 1/SIRT1 Monoclonal Antibody (Catalog # MAB7714) at 25 µg/mL for 1 hour at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules." class="big_lightbox"> MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules." class="big_thumb" />