Products Related to Gene-ID: 2252

345-FG), Recombinant Human Noggin (6057-NG), and Recombinant Human R-Spondin 1 (4645-RS), along with the other reagents listed in the lung organoid expansion medium recipe in the lung organoid culture protocol. Lung organoids were able to differentiate and exhibit markers for various cell types of the lung. Lung organoids were stained with (A) a Rabbit Anti-Human Cytokeratin 5 (KRT5) Monoclonal Antibody (Novus Biologicals, Catalog # NB110-56916; green) and a Goat Anti-Human p63/TP73L Polyclonal Antibody (AF1916; red) to visualize basal cells, (B) a Hamster Anti-Mouse Podoplanin (PDPN) Monoclonal Antibody (Novus Biologicals, Catalog # NB600-1015; green) to visualize alveolar type I cells and a Goat Anti-Human p63/TP73L Polyclonal Antibody (AF1916; red) to visualize basal cells, and (C, D) a Mouse Anti-MUC5AC Monoclonal Antibody (Novus Biologicals, Catalog # NBP2-15196; green) to visualize Goblet cells and a Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (MAB2018; red). All samples were counterstained with DAPI (5748; blue). " class="big_lightbox"> 345-FG), Recombinant Human Noggin (6057-NG), and Recombinant Human R-Spondin 1 (4645-RS), along with the other reagents listed in the lung organoid expansion medium recipe in the lung organoid culture protocol. Lung organoids were able to differentiate and exhibit markers for various cell types of the lung. Lung organoids were stained with (A) a Rabbit Anti-Human Cytokeratin 5 (KRT5) Monoclonal Antibody (Novus Biologicals, Catalog # NB110-56916; green) and a Goat Anti-Human p63/TP73L Polyclonal Antibody (AF1916; red) to visualize basal cells, (B) a Hamster Anti-Mouse Podoplanin (PDPN) Monoclonal Antibody (Novus Biologicals, Catalog # NB600-1015; green) to visualize alveolar type I cells and a Goat Anti-Human p63/TP73L Polyclonal Antibody (AF1916; red) to visualize basal cells, and (C, D) a Mouse Anti-MUC5AC Monoclonal Antibody (Novus Biologicals, Catalog # NBP2-15196; green) to visualize Goblet cells and a Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (MAB2018; red). All samples were counterstained with DAPI (5748; blue). " class="big_thumb" />

251-KG) stimulates proliferation in the 4MBr‑5 rhesus monkey epithelial cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human KGF/FGF‑7 (125 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human KGF/FGF‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-251-NA). The ND₅₀ is typically 6-12 µg/mL." class="big_lightbox"> 251-KG) stimulates proliferation in the 4MBr‑5 rhesus monkey epithelial cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human KGF/FGF‑7 (125 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human KGF/FGF‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-251-NA). The ND₅₀ is typically 6-12 µg/mL." alt="Recombinant Human KGF/FGF‑7 (Catalog # 251-KG) stimulates proliferation in the 4MBr‑5 rhesus monkey epithelial cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human KGF/FGF‑7 (125 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human KGF/FGF‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-251-NA). The ND₅₀ is typically 6-12 µg/mL." class="big_thumb" />

CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to trophoblast cells in chorionic villi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to trophoblast cells in chorionic villi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

251-KG) was serially diluted and captured by Mouse Anti-Human KGF/FGF‑7 Monoclonal Antibody (Catalog # MAB251) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human KGF/FGF‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-251-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 251-KG) was serially diluted and captured by Mouse Anti-Human KGF/FGF‑7 Monoclonal Antibody (Catalog # MAB251) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human KGF/FGF‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-251-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

251-KG) stimulates proliferation in the 4MBr-5 rhesus monkey epithelial cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human KGF/FGF-7 (100 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human KGF/FGF-7 Monoclonal Antibody (Catalog # MAB251). The ND₅₀ is typically 0.1-0.5 µg/mL." class="big_lightbox"> 251-KG) stimulates proliferation in the 4MBr-5 rhesus monkey epithelial cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human KGF/FGF-7 (100 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human KGF/FGF-7 Monoclonal Antibody (Catalog # MAB251). The ND₅₀ is typically 0.1-0.5 µg/mL." alt="Recombinant Human KGF/FGF-7 (Catalog # 251-KG) stimulates proliferation in the 4MBr-5 rhesus monkey epithelial cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human KGF/FGF-7 (100 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human KGF/FGF-7 Monoclonal Antibody (Catalog # MAB251). The ND₅₀ is typically 0.1-0.5 µg/mL." class="big_thumb" />

251-KG) was serially diluted and captured by Mouse Anti-Human KGF/FGF‑7 Monoclonal Antibody (Catalog # MAB251) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human KGF/FGF‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-251-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 251-KG) was serially diluted and captured by Mouse Anti-Human KGF/FGF‑7 Monoclonal Antibody (Catalog # MAB251) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human KGF/FGF‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-251-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

BT-KGF-AFL) grades of RecombinantHuman KGF/FGF-7 measured in a cell proliferationassay using Ba/F3 mouse pro Bcells transfected with human FGFRIIb (orange and green, respectively)." class="big_lightbox"> BT-KGF-AFL) grades of RecombinantHuman KGF/FGF-7 measured in a cell proliferationassay using Ba/F3 mouse pro Bcells transfected with human FGFRIIb (orange and green, respectively)." class="big_thumb" />

BT-KGF, BT-KGF-AFL, and BT-KGF-GMP). The iPSC cells (1210a cell line) were cultured in a 24-well plate and differentiated into pancreatic progenitor cells using different lots of KGF and other differentiation factors. Expression of pancreatic progenitor markers was analyzed via flow cytometry with anti-PDX-1 (Catalog # MAB2419) and anti-NKX6.1 antibodies. The PDX-1+/NKX6.1+ double positive cell population was quantified as the pancreatic progenitor cells. The data is represented as the average of three technical replicates." class="big_lightbox"> BT-KGF, BT-KGF-AFL, and BT-KGF-GMP). The iPSC cells (1210a cell line) were cultured in a 24-well plate and differentiated into pancreatic progenitor cells using different lots of KGF and other differentiation factors. Expression of pancreatic progenitor markers was analyzed via flow cytometry with anti-PDX-1 (Catalog # MAB2419) and anti-NKX6.1 antibodies. The PDX-1+/NKX6.1+ double positive cell population was quantified as the pancreatic progenitor cells. The data is represented as the average of three technical replicates." class="big_thumb" />

MAB14171) and the anti-somatostatin antibody (Catalog # MAB2358), followed by the secondary antibody staining (anti-mouse IgG NL493-conjugated antibody: Catalog # NL009; anti-rat IgG NL557-conjugated antibody: Catalog # NL013). A Leica THUNDER Imaging System was utilized for the imaging of C-peptide and somatostatin expression in the beta islets. GMP KGF successfully differentiates the iPSCs into functional beta islets capable of expressing these characteristic markers. " class="big_lightbox"> MAB14171) and the anti-somatostatin antibody (Catalog # MAB2358), followed by the secondary antibody staining (anti-mouse IgG NL493-conjugated antibody: Catalog # NL009; anti-rat IgG NL557-conjugated antibody: Catalog # NL013). A Leica THUNDER Imaging System was utilized for the imaging of C-peptide and somatostatin expression in the beta islets. GMP KGF successfully differentiates the iPSCs into functional beta islets capable of expressing these characteristic markers. " class="big_thumb" />

CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to chorionic villi trophoblasts. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to chorionic villi trophoblasts. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="KGF/FGF-7 was detected in immersion fixed paraffin-embedded sections of canine placenta (chorionic villi) using Mouse Anti-Canine KGF/FGF-7 Monoclonal Antibody (Catalog # MAB1957) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to chorionic villi trophoblasts. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

BT-KGF-GMP), Animal-Free (Catalog # BT-KGF-AFL) and RUO (BT-KGF) grades of Recombinant Human KGF/FGF-7 GMP protein as measured in a cell proliferation assay using Ba/F3 mouse pro B cells transfected with human FGF RIIb (orange, green, red, respectively). " class="big_lightbox"> BT-KGF-GMP), Animal-Free (Catalog # BT-KGF-AFL) and RUO (BT-KGF) grades of Recombinant Human KGF/FGF-7 GMP protein as measured in a cell proliferation assay using Ba/F3 mouse pro B cells transfected with human FGF RIIb (orange, green, red, respectively). " alt="Equivalent bioactivity of GMP (BT-KGF-GMP), Animal-Free (Catalog # BT-KGF-AFL) and RUO (BT-KGF) grades of Recombinant Human KGF/FGF-7 GMP protein as measured in a cell proliferation assay using Ba/F3 mouse pro B cells transfected with human FGF RIIb (orange, green, red, respectively). " class="big_thumb" />