Immunoprecipitation (IP) is a common research technique used to precipitate a specific protein (or protein complex) of interest out of a heterogeneous lysate. The concept of precipitating proteins out of solution is often called a "pull-down". There are a variety of methods to achieve immunoprecipitation, the most common of which uses antibodies bound to magnetic or agarose beads. This process can be used to isolate and concentrate a particular protein or complex from a sample containing many thousands of different proteins, and is often used to study protein-protein interactions through Co-IP experiments.
Novus offers a range of support options for IP when you buy a product to help you with your IP experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF6394-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB4364-SP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 350 | AF2649U-100UG | |||
| Alexa Fluor 405 | AF2649V-100UG | |||
| Alexa Fluor 488 | AF2649G-100UG | |||
| Alexa Fluor 532 | AF2649X-100UG | |||
| Alexa Fluor 594 | AF2649T-100UG | |||
| Alexa Fluor 647 | AF2649R-100UG | |||
| Alexa Fluor 700 | AF2649N-100UG | |||
| Alexa Fluor 750 | AF2649S-100UG | |||
| Biotin | BAF2649 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2649-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB4099-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB2666-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2079SS | |||
| Azide and BSA Free | NBP2-80732 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-81927-0.025ml | |||
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-HCF1 antibody NB100-68209 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. HCF1 was also immunoprecipitated by rabbit anti-HCF1 antibody NB100-68210, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/Host-Cell-Factor-1-HCFC1-Antibody-Western-Blot-NB100-68209-img0010.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-HCF1 antibody NB100-68209 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. HCF1 was also immunoprecipitated by rabbit anti-HCF1 antibody NB100-68210, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).")
![<b>Independent Antibodies Validation and Genetic Strategies Validation.</b>Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - Expression pattern of HCF1, MLL, and Nrf2 in SNUC5/5-FUR cells was determined by Western blot analysis. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.9745) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Host-Cell-Factor-1-HCFC1-Antibody-Knockdown-Validated-NB100-68209-img0011.jpg)
![Immunoprecipitation: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - Interaction between MLL and HCF1was examined by immunoprecipitation analysis using anti-MLL and anti-HCF1 antibodies followed by Western blotting with anti-HCF1 and anti-MLL antibodies. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.9745) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Host-Cell-Factor-1-HCFC1-Antibody-Immunoprecipitation-NB100-68209-img0012.jpg)
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - 5-FU-produced ROS involved in histone methyltransferase-mediated Nrf2 expression. E. Expression patterns of TET1, OGT, HCF1, & MLL in NAC pre-treated SNUC5/5-FUR cells determined by WB analysis. *Significantly different from SNUC5 cells (p<0.05), & #significantly different from SNUC5/5-FUR cells (p<0.05). Also, SNUC5 cells pre-treated with 500 μM of NAC & incubated for an additional 1 h at 37°. Cells then treated with 140 μM 5-FU for 48 h. 10 μM of DHR123 was added to plate & incubated for an additional 30 min at 37°C. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.9745), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68209_rabbit-polyclonal-host-cell-factor-1-hcfc1-antibody-310202415171343.jpg)
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - 5-FU-produced ROS involved in histone methyltransferase-mediated Nrf2 expression. J. Expression of TET1 & interaction proteins detected by WB analysis. *Significantly different from SNUC5 cells (p<0.05), & #significantly different from 5-FU-treated SNUC5 cells (p<0.05). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.9745), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68209_rabbit-polyclonal-host-cell-factor-1-hcfc1-antibody-310202415345312.jpg)
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - 5-FU-produced ROS involved in histone methyltransferase-mediated Nrf2 expression. F. The intracellular ROS level was detected by flow cytometry. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.9745), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68209_rabbit-polyclonal-host-cell-factor-1-hcfc1-antibody-31020241534395.jpg)
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - Effect of MLL-HCF1 interaction on histone methylation & Nrf2 expression in SNUC5/5-FUR cellsA. HCF1 expression was determined by Western blotting. *Significantly different from SNUC5 cells (p<0.05). B. Interaction between MLL & HCF1was examined by immunoprecipitation analysis using anti-MLL & anti-HCF1 antibodies followed by Western blotting with anti-HCF1 & anti-MLL antibodies. *Significantly different from SNUC5 cells (p<0.05). C. Interaction between MLL & HCF1was assessed by PLA. Each green spot represents for a single interaction (MLL & HCF1) & DNA was stained with DAPI. *Significantly different from SNUC5 cells (p<0.05). The cells were transfected with non-targeting siRNA (siControl) or siHCF1 RNA for 24 h. D. Expression pattern of MLL mRNA in SNUC5/5-FUR cells was determined by RT-PCR analysis. *Significantly different from siControl-transfected cells (p<0.05). E. Expression pattern of HCF1, MLL, & Nrf2 in SNUC5/5-FUR cells was determined by Western blot analysis. *Significantly different from siControl-transfected cells (p<0.05). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.9745), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68209_rabbit-polyclonal-host-cell-factor-1-hcfc1-antibody-310202415532061.jpg)
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - Relation of OGT-TET1 interaction on histone methylation & Nrf2 expression in SNUC5/5-FUR cellsA. Interaction between OGT & TET1 was examined by immunoprecipitation analyses using anti-OGT & anti-TET1 antibodies followed by Western blotting with anti-TET1 & anti-OGT antibodies. B. Interaction between OGT & TET1 was assessed by using PLA. Each green spot represents for a single interaction (OGT & TET1) & DNA was stained with DAPI. *Significantly different from SNUC5 cells (p<0.05). C. The O-GlcNAcylated TET1 or O-GlcNAcylated OGT was examined by immunoprecipitation analyses using anti-O-GlcNAc antibody followed by Western blotting with anti-TET1 & anti-OGT antibodies. The cells were transfected with non-targeting siRNA (siControl) or siOGT RNA or siTET1 RNA for 24 h. D. Expression pattern of OGT & TET1 in SNUC5/5-FUR cells was determined by Western blot analysis. *Significantly different from siControl-transfected cells (p<0.05). E. Expression pattern of TET1, OGT, HCF1, MLL, & Nrf2 in SNUC5/5-FUR cells was determined by Western blot analysis. *Significantly different from siControl-transfected cells (p<0.05). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.9745), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68209_rabbit-polyclonal-host-cell-factor-1-hcfc1-antibody-310202415523922.jpg)
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - Involvement of OGT in histone methylase MLL/COMPASS-like complex-mediated Nrf2 expression in SNUC5/5-FUR cellsA. OGT & O-GlcNAc expression was determined by Western blotting. *Significantly different from SNUC5 cells (p<0.05). B. Interaction between HCF1 & OGT was examined by immunoprecipitation analysis using anti-HCF1 & anti-OGT antibodies followed by Western blotting with anti-HCF1 & anti-OGT antibodies. C. Interaction between HCF1 & OGT was assessed by using PLA. Each green spot represents for a single interaction (HCF1 & OGT) & DNA was stained with DAPI. *Significantly different from SNUC5 cells (p<0.05). D. Interaction between MLL & OGT was examined by immunoprecipitation analysis using anti-MLL & anti-OGT antibodies followed by Western blotting with anti-OGT & anti-MLL antibodies. E. Interaction between MLL & OGT was assessed by using PLA. Each green spot represents for a single interaction (MLL & OGT) & DNA was stained with DAPI. *Significantly different from SNUC5 cells (p<0.05). The cells were transfected with non-targeting siRNA (siControl) or siOGT RNA for 24 h. F. Expression pattern of MLL mRNA in SNUC5/5-FUR cells was determined by RT-PCR analysis. *Significantly different from siControl-transfected cells (p<0.05). G. Expression pattern of OGT, MLL, HFC1, & Nrf2 in SNUC5/5-FUR cells was determined by Western blot analysis. *Significantly different from siControl-transfected cells (p<0.05). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.9745), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68209_rabbit-polyclonal-host-cell-factor-1-hcfc1-antibody-310202415532084.jpg)
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - Involvement of OGT in histone methylase MLL/COMPASS-like complex-mediated Nrf2 expression in SNUC5/5-FUR cellsA. OGT & O-GlcNAc expression was determined by Western blotting. *Significantly different from SNUC5 cells (p<0.05). B. Interaction between HCF1 & OGT was examined by immunoprecipitation analysis using anti-HCF1 & anti-OGT antibodies followed by Western blotting with anti-HCF1 & anti-OGT antibodies. C. Interaction between HCF1 & OGT was assessed by using PLA. Each green spot represents for a single interaction (HCF1 & OGT) & DNA was stained with DAPI. *Significantly different from SNUC5 cells (p<0.05). D. Interaction between MLL & OGT was examined by immunoprecipitation analysis using anti-MLL & anti-OGT antibodies followed by Western blotting with anti-OGT & anti-MLL antibodies. E. Interaction between MLL & OGT was assessed by using PLA. Each green spot represents for a single interaction (MLL & OGT) & DNA was stained with DAPI. *Significantly different from SNUC5 cells (p<0.05). The cells were transfected with non-targeting siRNA (siControl) or siOGT RNA for 24 h. F. Expression pattern of MLL mRNA in SNUC5/5-FUR cells was determined by RT-PCR analysis. *Significantly different from siControl-transfected cells (p<0.05). G. Expression pattern of OGT, MLL, HFC1, & Nrf2 in SNUC5/5-FUR cells was determined by Western blot analysis. *Significantly different from siControl-transfected cells (p<0.05). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.9745), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68209_rabbit-polyclonal-host-cell-factor-1-hcfc1-antibody-310202415525241.jpg)
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - Effect of MLL-HCF1 interaction on histone methylation & Nrf2 expression in SNUC5/5-FUR cellsA. HCF1 expression was determined by Western blotting. *Significantly different from SNUC5 cells (p<0.05). B. Interaction between MLL & HCF1was examined by immunoprecipitation analysis using anti-MLL & anti-HCF1 antibodies followed by Western blotting with anti-HCF1 & anti-MLL antibodies. *Significantly different from SNUC5 cells (p<0.05). C. Interaction between MLL & HCF1was assessed by PLA. Each green spot represents for a single interaction (MLL & HCF1) & DNA was stained with DAPI. *Significantly different from SNUC5 cells (p<0.05). The cells were transfected with non-targeting siRNA (siControl) or siHCF1 RNA for 24 h. D. Expression pattern of MLL mRNA in SNUC5/5-FUR cells was determined by RT-PCR analysis. *Significantly different from siControl-transfected cells (p<0.05). E. Expression pattern of HCF1, MLL, & Nrf2 in SNUC5/5-FUR cells was determined by Western blot analysis. *Significantly different from siControl-transfected cells (p<0.05). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.9745), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68209_rabbit-polyclonal-host-cell-factor-1-hcfc1-antibody-310202415523923.jpg)
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - Effect of MLL-HCF1 interaction on histone methylation & Nrf2 expression in SNUC5/5-FUR cellsA. HCF1 expression was determined by Western blotting. *Significantly different from SNUC5 cells (p<0.05). B. Interaction between MLL & HCF1was examined by immunoprecipitation analysis using anti-MLL & anti-HCF1 antibodies followed by Western blotting with anti-HCF1 & anti-MLL antibodies. *Significantly different from SNUC5 cells (p<0.05). C. Interaction between MLL & HCF1was assessed by PLA. Each green spot represents for a single interaction (MLL & HCF1) & DNA was stained with DAPI. *Significantly different from SNUC5 cells (p<0.05). The cells were transfected with non-targeting siRNA (siControl) or siHCF1 RNA for 24 h. D. Expression pattern of MLL mRNA in SNUC5/5-FUR cells was determined by RT-PCR analysis. *Significantly different from siControl-transfected cells (p<0.05). E. Expression pattern of HCF1, MLL, & Nrf2 in SNUC5/5-FUR cells was determined by Western blot analysis. *Significantly different from siControl-transfected cells (p<0.05). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.9745), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68209_rabbit-polyclonal-host-cell-factor-1-hcfc1-antibody-310202415533831.jpg)
![Western Blot: G3BP1 Antibody [NBP1-18922] - Whole cell lysate (50 ug) from HeLa, MCF-7, U2OS, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-G3BP1antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with anexposure time of 1 second.](http://images.novusbio.com/fullsize/G3BP1-Antibody-Western-Blot-NBP1-18922-img0008.jpg "Western Blot: G3BP1 Antibody [NBP1-18922] - Whole cell lysate (50 ug) from HeLa, MCF-7, U2OS, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-G3BP1antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with anexposure time of 1 second.")
![Immunocytochemistry/Immunofluorescence: G3BP1 Antibody [NBP1-18922] - G3BP1 in red (Alexa fluor 555) and nucleus in blue (DAPI). Human primary fibroblasts untreated in the left and treated in the right with G3BP1 stress granules labeling. Alexa fluor 555 1s exposure and DAPI 1s exposure. Fixation: PFA 4% 10min RT, permeabilization: Triton 0.1% TBS, blocking: Donkey Serum 10% TBS-T, Ab dilution: 1:1000, Secondary Ab: Alexa fluor 555 Donkey anti-rabbit 1:600, Mounting medium with DAPI. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/G3BP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-18922-img0007.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: G3BP1 Antibody [NBP1-18922] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50mcg) cells. Affinity purified rabbit anti-G3BP1 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. G3BP1 was also immunoprecipitated by rabbit anti-G3BP1 antibody NBP1-18923, which recognizes a downstream epitope.](http://images.novusbio.com/fullsize/G3BP1-Antibody-Western-Blot-NBP1-18922-img0006.jpg)
![Immunohistochemistry-Paraffin: SIN3B Antibody [NBP2-20367] - MCF7 xenograft, using SIN3B antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/SIN3B-Antibody-Immunohistochemistry-Paraffin-NBP2-20367-img0005.jpg "Immunohistochemistry-Paraffin: SIN3B Antibody [NBP2-20367] - MCF7 xenograft, using SIN3B antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Immunoprecipitation: SIN3B Antibody [NBP2-20367] - Immunoprecipitation of SIN3B protein from HCT-116 whole cell extracts using 5 ug of SIN3B antibody [N1N2], N-term Western blot analysis was performed using SIN3B antibody [N1N2], N-term. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/SIN3B-Antibody-Immunoprecipitation-NBP2-20367-img0008.jpg)
![Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with SIN3B antibody [N1N2], N-term (NBP2-20367) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-20367_rabbit-polyclonal-sin3b-antibody-western-blot-261120251824165.jpg)
![SIN3B antibody [N1N2], N-term detects SIN3B protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SIN3B protein stained by SIN3B antibody [N1N2], N-term (NBP2-20367) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp2-20367_rabbit-polyclonal-sin3b-antibody-immunocytochemistry-immunofluorescence-261120251827460.jpg)
![Western Blot: FGF R1 Antibody (M17A3) [NB100-2079] - Whole cell protein from HepG2 (lane 1), 3T3 (lane 2) and PC12 (lane 3) was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 4.0 ug/ml anti-FGFR1 in 1% milk and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/FGF-R1-Antibody-M17A3-Western-Blot-NB100-2079-img0004.jpg "Western Blot: FGF R1 Antibody (M17A3) [NB100-2079] - Whole cell protein from HepG2 (lane 1), 3T3 (lane 2) and PC12 (lane 3) was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 4.0 ug/ml anti-FGFR1 in 1% milk and detected with an anti-mouse HRP secondary antibody using chemiluminescence.")
![Immunohistochemistry-Paraffin: FGF R1 Antibody (M17A3) [NB100-2079] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human normal skin using mouse monoclonal FGF R1 Antibody (clone M17A3) at 5 ug/ml concentration. The keratinocytes of the epidermal layer showed a very strong membrane-cytoplasmic with relatively weak nuclear staining for FGF R1 Antibody. The stratum basale layer of keratinocytes depicted more of membrane-cytoplasmic staining for this protein.](http://images.novusbio.com/fullsize/FGF-R1-Antibody-M17A3-Immunohistochemistry-Paraffin-NB100-2079-img0003.jpg)
![Flow Cytometry: FGF R1 Antibody (M17A3) [NB100-2079] - Analysis of FGF R1 in HEK293 cells (2x10^6 cells/ml) were stained with FGF R1 antibody (NB100-2079, red) at 1:1000 dilution. Detected with FITC conjugated goat anti-mouse IgG1 isotype control (green). Two distinct samples shown.](http://images.novusbio.com/fullsize/FGF-R1-Antibody-M17A3-Flow-Cytometry-NB100-2079-img0006.jpg)
![Flow Cytometry: FGF R1 Antibody (M17A3) [NB100-2079] - Analysis of FGF R1 in MCF-7 cells (1x10^6 cells/ml) were stained with FGF R1 antibody (NB100-2079, red) at 1:1000 dilution. Detected with FITC conjugated goat anti-mouse IgG1 isotype control (green). Two distinct samples shown.](http://images.novusbio.com/fullsize/FGF-R1-Antibody-M17A3-Flow-Cytometry-NB100-2079-img0005.jpg)
![Immunohistochemistry-Paraffin: FoxO1/FKHR [p Ser256] Antibody [NB100-81927] - Immunohistochemical staining in human lung cancer formalin fixed paraffin-embedded tissue section.](http://images.novusbio.com/fullsize/nb100-81927_rabbit-polyclonal-foxo1-fkhr-p-ser256-antibody-2342024155517.jpg "Immunohistochemistry-Paraffin: FoxO1/FKHR [p Ser256] Antibody [NB100-81927] - Immunohistochemical staining in human lung cancer formalin fixed paraffin-embedded tissue section.")
![Immunocytochemistry/ Immunofluorescence: FoxO1/FKHR [p Ser256] Antibody [NB100-81927] - Immunofluorescent staining in MCF7 cells.](http://images.novusbio.com/fullsize/nb100-81927_rabbit-polyclonal-foxo1-fkhr-p-ser256-antibody-234202415232510.jpg)
![Western Blot: PTBP1 Antibody (3H8) [H00005725-M01] - Analysis of PTBP1 expression in transfected 293T cell line by PTBP1 monoclonal antibody (M01), clone 3H8.Lane 1: PTBP1 transfected lysate(59.6 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PTBP1-Antibody-3H8-Western-Blot-H00005725-M01-img0019.jpg "Western Blot: PTBP1 Antibody (3H8) [H00005725-M01] - Analysis of PTBP1 expression in transfected 293T cell line by PTBP1 monoclonal antibody (M01), clone 3H8.Lane 1: PTBP1 transfected lysate(59.6 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: PTBP1 Antibody (3H8) [H00005725-M01] - Analysis of monoclonal antibody to PTBP1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/PTBP1-Antibody-3H8-Immunocytochemistry-Immunofluorescence-H00005725-M01-img0013.jpg)
![Immunohistochemistry-Paraffin: PTBP1 Antibody (3H8) [H00005725-M01] - Analysis of monoclonal antibody to PTBP1 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/PTBP1-Antibody-3H8-Immunohistochemistry-Paraffin-H00005725-M01-img0015.jpg)
![Western Blot: PTBP1 Antibody (3H8) [H00005725-M01] - 2 minute exposure for Mouse embryonic stem cell - V6.5 with a dilution of 1:500 - Courtesy of Paul Fields at MIT](http://images.novusbio.com/fullsize/PTBP1-Antibody-3H8-Western-Blot-H00005725-M01-img0008.jpg)
![Western Blot: PTBP1 Antibody (3H8) [H00005725-M01] - 5 minute exposure for Mouse embryonic stem cell - V6.5 with a dilution of 1:500 - Courtesy of Paul Fields at MIT](http://images.novusbio.com/fullsize/PTBP1-Antibody-3H8-Western-Blot-H00005725-M01-img0009.jpg)
![Western Blot: PTBP1 Antibody (3H8) [H00005725-M01] - PTBP1 monoclonal antibody (M01), clone 3H8 Analysis of PTBP1 expression in A-431.](http://images.novusbio.com/fullsize/PTBP1-Antibody-3H8-Western-Blot-H00005725-M01-img0018.jpg)
![Immunoprecipitation: PTBP1 Antibody (3H8) [H00005725-M01] - Analysis of PTBP1 transfected lysate using anti-PTBP1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PTBP1 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/PTBP1-Antibody-3H8-Immunoprecipitation-H00005725-M01-img0016.jpg)
![ELISA: PTBP1 Antibody (3H8) [H00005725-M01] - Detection limit for recombinant GST tagged PTBP1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PTBP1-Antibody-3H8-ELISA-H00005725-M01-img0020.jpg)
![Western Blot: KIF2C Antibody (1G2) [H00011004-M01] - KIF2C monoclonal antibody (M01), clone 1G2 Analysis of KIF2C expression in Hela S3 NE.](http://images.novusbio.com/fullsize/KIF2C-Antibody-1G2-Western-Blot-H00011004-M01-img0014.jpg "Western Blot: KIF2C Antibody (1G2) [H00011004-M01] - KIF2C monoclonal antibody (M01), clone 1G2 Analysis of KIF2C expression in Hela S3 NE.")
![Immunocytochemistry/Immunofluorescence: KIF2C Antibody (1G2) [H00011004-M01] - Analysis of monoclonal antibody to KIF2C on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/KIF2C-Antibody-1G2-Immunocytochemistry-Immunofluorescence-H00011004-M01-img0008.jpg)
![Immunohistochemistry-Paraffin: KIF2C Antibody (1G2) [H00011004-M01] - Analysis of monoclonal antibody to KIF2C on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/KIF2C-Antibody-1G2-Immunohistochemistry-Paraffin-H00011004-M01-img0010.jpg)
![Western Blot: KIF2C Antibody (1G2) [H00011004-M01] - Analysis of KIF2C over-expressed 293 cell line, cotransfected with KIF2C Validated Chimera RNAi ( Cat # H00011004-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with KIF2C monoclonal antibody (M01), clone 1G2 (Cat # H00011004-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/KIF2C-Antibody-1G2-Western-Blot-H00011004-M01-img0012.jpg)
![Western Blot: KIF2C Antibody (1G2) [H00011004-M01] - Analysis of KIF2C expression in transfected 293T cell line by KIF2C monoclonal antibody (M01), clone 1G2.Lane 1: KIF2C transfected lysate(81.3 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/KIF2C-Antibody-1G2-Western-Blot-H00011004-M01-img0015.jpg)
![Immunoprecipitation: KIF2C Antibody (1G2) [H00011004-M01] - Analysis of KIF2C transfected lysate using anti-KIF2C monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with KIF2C MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/KIF2C-Antibody-1G2-Immunoprecipitation-H00011004-M01-img0011.jpg)
![Sandwich ELISA: KIF2C Antibody (1G2) [H00011004-M01] - Detection limit for recombinant GST tagged KIF2C is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/KIF2C-Antibody-1G2-Sandwich-ELISA-H00011004-M01-img0013.jpg)