Immunoprecipitation (IP) is a common research technique used to precipitate a specific protein (or protein complex) of interest out of a heterogeneous lysate. The concept of precipitating proteins out of solution is often called a "pull-down". There are a variety of methods to achieve immunoprecipitation, the most common of which uses antibodies bound to magnetic or agarose beads. This process can be used to isolate and concentrate a particular protein or complex from a sample containing many thousands of different proteins, and is often used to study protein-protein interactions through Co-IP experiments.
Novus offers a range of support options for IP when you buy a product to help you with your IP experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-42186-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2623-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52405-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54625-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2724-0.025mg | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alkaline Phosphatase | NBP1-74819 | |||
| Biotin | NBP1-74820 | |||
| DyLight 488 | NBP1-74824 | |||
| DyLight 550 | NBP1-75606 | |||
| DyLight 594 | NBP1-75607 | |||
| DyLight 650 | NBP1-75609 | |||
| FITC | NBP1-74821 | |||
| HRP | NBP1-74822 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Pre-adsorbed | NBP1-74819 | |||
| Pre-adsorbed | NBP1-74820 | |||
| Pre-adsorbed | NBP1-74821 | |||
| Pre-adsorbed | NBP1-74822 | |||
| Pre-adsorbed | NBP1-74824 | |||
| Pre-adsorbed | NBP1-75606 | |||
| Pre-adsorbed | NBP1-75607 | |||
| Pre-adsorbed | NBP1-75609 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alkaline Phosphatase | NBP1-75301 | |||
| Biotin | NBP1-75302 | |||
| DyLight 488 | NBP1-75306 | |||
| DyLight 550 | NBP1-76060 | |||
| DyLight 594 | NBP1-76061 | |||
| DyLight 633 | NBP1-76062 | |||
| DyLight 650 | NBP1-76063-0.1mg | |||
| FITC | NBP1-75303 | |||
| HRP | NBP1-75304-0.1mg | |||
| TRITC | NBP1-75305-1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Pre-adsorbed | NBP1-75301 | |||
| Pre-adsorbed | NBP1-75302 | |||
| Pre-adsorbed | NBP1-75303 | |||
| Pre-adsorbed | NBP1-75306 | |||
| Pre-adsorbed | NBP1-76060 | |||
| Pre-adsorbed | NBP1-76061 | |||
| Pre-adsorbed | NBP1-76062 | |||
| Pre-adsorbed | NBP1-75305-1mg | |||
| Pre-adsorbed | NBP1-76063-0.1mg | |||
| Pre-adsorbed | NBP1-75304-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Pre-adsorbed | NBP1-74876 | |||
| Pre-adsorbed | NBP1-74877 | |||
| Pre-adsorbed | NBP1-74878 | |||
| Pre-adsorbed | NBP1-74880 | |||
| Pre-adsorbed | NBP1-74874-0.5mg | |||
| Pre-adsorbed | NBP1-74874JF549 | |||
| Pre-adsorbed | NBP1-74874JF646 | |||
| Pre-adsorbed | NBP1-75746-1000ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97446-0.025mg | |||
![Western Blot: OLIG1 Antibody [NBP1-28666] - Neonatal rat brain lysate showing specific immunolabeling of the ~ 27k Olig1 protein.](http://images.novusbio.com/fullsize/OLIG1-Antibody-Western-Blot-NBP1-28666-img0001.jpg "Western Blot: OLIG1 Antibody [NBP1-28666] - Neonatal rat brain lysate showing specific immunolabeling of the ~ 27k Olig1 protein.")
![Immunocytochemistry/Immunofluorescence: Olig1 Antibody [NBP1-28666] - Immunostaining of primary rat oligodendrocytes using anti-Olig1.](http://images.novusbio.com/fullsize/Olig1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-28666-img0002.jpg)
![Western Blot: Ataxin 1 Antibody (S76-8) [NBP2-42186] - Western Blot analysis of Monkey COS-1 cells transfected with Ataxin- 1 showing detection of ~85 kDa Ataxin 1 protein using Mouse Anti-Ataxin 1 Monoclonal Antibody, Clone S76-8 (NBP2-42186). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS-1 cells transfected with Ataxin- 1. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Ataxin 1 Monoclonal Antibody (NBP2-42186) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~85 kDa.](http://images.novusbio.com/fullsize/Ataxin-1-Antibody-S76-8-Western-Blot-NBP2-42186-img0002.jpg "Western Blot: Ataxin 1 Antibody (S76-8) [NBP2-42186] - Western Blot analysis of Monkey COS-1 cells transfected with Ataxin- 1 showing detection of ~85 kDa Ataxin 1 protein using Mouse Anti-Ataxin 1 Monoclonal Antibody, Clone S76-8 (NBP2-42186). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS-1 cells transfected with Ataxin- 1. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Ataxin 1 Monoclonal Antibody (NBP2-42186) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~85 kDa.")
![Immunocytochemistry/Immunofluorescence: Ataxin 1 Antibody (S76-8) [NBP2-42186] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ataxin 1 Monoclonal Antibody, Clone S76-8 (NBP2-42186). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Ataxin 1 Monoclonal Antibody (NBP2-42186) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Ataxin 1 Antibody (D) Composite.](http://images.novusbio.com/fullsize/Ataxin-1-Antibody-S76-8-Immunocytochemistry-Immunofluorescence-NBP2-42186-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Ataxin 1 Antibody (S76-8) [NBP2-42186] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ataxin 1 Monoclonal Antibody, Clone S76-8 (NBP2-42186). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ataxin 1 Monoclonal Antibody (NBP2-42186) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Ataxin 1 Antibody. (D) Composite.](http://images.novusbio.com/fullsize/Ataxin-1-Antibody-S76-8-Immunocytochemistry-Immunofluorescence-NBP2-42186-img0004.jpg)
![Western Blot: BORIS Antibody (20B11) [NBP2-52405] - Antibody from clone 20B11 specifically recognises the C terminal domain of BORIS, and the endogenous and exogenous BORIS protein. Cell lysates were resolved by SDS-PAGE, blotted and probed with the original mouse supernatant of the 20B11 clone. Positions of BORIS and the C-terminal domain of BORIS are indicated. B, bacterially expressed BORIS; 293T cells transfected with 0.5ug of the plasmid expressing BORIS; M, Molecular marker.](http://images.novusbio.com/fullsize/BORIS-Antibody-20B11-Western-Blot-NBP2-52405-img0006.jpg "Western Blot: BORIS Antibody (20B11) [NBP2-52405] - Antibody from clone 20B11 specifically recognises the C terminal domain of BORIS, and the endogenous and exogenous BORIS protein. Cell lysates were resolved by SDS-PAGE, blotted and probed with the original mouse supernatant of the 20B11 clone. Positions of BORIS and the C-terminal domain of BORIS are indicated. B, bacterially expressed BORIS; 293T cells transfected with 0.5ug of the plasmid expressing BORIS; M, Molecular marker.")
![Immunohistochemistry: BORIS Antibody (20B11) [NBP2-52405] - Analysis using the supernatants from the clone 20B11 at following dilutions: (A) 20B11 undiluted supernatant and (B) 20B11 at 1:25. (B) Cells have been counterstained with the nuclear marker hematoxylin (blue). Images were taken at x60 magnification using the Olympus BX41 microscope. Expression of BORIS protein is indicated by the presence of brown staining, with a pattern characteristic for BORIS ( both, cytoplasmic and nuclear).](http://images.novusbio.com/fullsize/BORIS-Antibody-20B11-Immunohistochemistry-NBP2-52405-img0003.jpg)
![Immunoprecipitation: BORIS Antibody (20B11) [NBP2-52405] - The 83-kDa protein can be immunoprecipitated with the mouse anti- BORIS C terminal specific antibody. Proteins were immunoprecipitated from the MCF7 cell extracts with the mouse anti- BORIS C terminal antibody 20B11 in their original supernatant form using the following volumes: (1) 400 ul of 20B11; (2) 800 ul of 20B11; (3) Input; (4) MCF7 cell lysates as BORIS positive control. The extracts from MCF7 cells were incubated overnight at 40C followed by 2 hour incubation with protein G sepharose beads. Proteins were resolved by SDS-PAGE, and Western blot analysis was performed with the concentrated form of the mouse anti- BORIS N terminal (4A7) specific antibody (1:500).](http://images.novusbio.com/fullsize/BORIS-Antibody-20B11-Immunoprecipitation-NBP2-52405-img0005.jpg)
![Western Blot: KCC2/SLC12A5 [p Ser940] Antibody [NBP2-29513] - Rat hippocampal homogenate showing specific labeling of the ~ 135k KCC2 protein (Control). Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (Peptide) but not by the corresponding dephospho-peptide (not shown).](http://images.novusbio.com/fullsize/KCC2-SLC12A5-[p-Ser940]-Antibody-Western-Blot-NBP2-29513-img0002.jpg "Western Blot: KCC2/SLC12A5 [p Ser940] Antibody [NBP2-29513] - Rat hippocampal homogenate showing specific labeling of the ~ 135k KCC2 protein (Control). Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (Peptide) but not by the corresponding dephospho-peptide (not shown).")
![Western Blot: CEACAM-5/CD66e Antibody (5C8C4) [NBP2-54625] - Analysis of human granulocyte lysate: Detection using 10 ug/ml Anti-CEACAM5 [5C8C4] followed by HRP coupled secondary pAb. Visualized by Chemiluminescence detection.](http://images.novusbio.com/fullsize/CEACAM-5-CD66e-Antibody-5C8C4-Western-Blot-NBP2-54625-img0008.jpg "Western Blot: CEACAM-5/CD66e Antibody (5C8C4) [NBP2-54625] - Analysis of human granulocyte lysate: Detection using 10 ug/ml Anti-CEACAM5 [5C8C4] followed by HRP coupled secondary pAb. Visualized by Chemiluminescence detection.")
![Flow Cytometry: CEACAM-5/CD66e Antibody (5C8C4) [NBP2-54625] - Binding of 10 ug/ml primary positive control mAb (upper panel), Anti-CEACAM5 [5C8C4] (lower panel), negative isotype matched control mAb (thin line) on different CHO-human CEACAM transfected cells (the CEACAM family member expressed is stated above).](http://images.novusbio.com/fullsize/CEACAM-5-CD66e-Antibody-5C8C4-Flow-Cytometry-NBP2-54625-img0003.jpg)
![ELISA: CEACAM-5/CD66e Antibody (5C8C4) [NBP2-54625] - Solid phase was coated with 3 ug/ml Anti-CEA (Dako) binding human CEACAM1-CEACAM8. After washing, blocking and coating human CEACAM1 antigen, detecting antibody Anti-CEACAM5/8 [5C8C4] (10 ug/ml) followed by HRP-coupled goat anti-mouse Ig was added. TMB was used for visualizing the binding measured by Tecan-ELISA reader at 450 nm.](http://images.novusbio.com/fullsize/CEACAM-5-CD66e-Antibody-5C8C4-ELISA-NBP2-54625-img0002.jpg)
![Immunoprecipitation: CEACAM-5/CD66e Antibody (5C8C4) [NBP2-54625] - Human granulocyte lysate: Utilizing 10 ug/ml Anti-CEACAM5/8 [5C8C4]. Detection of the sample using 5 ug/ml pAb Anti-CEA followed by HRP coupled secondary pAb. Visualized by Chemiluminescence detection.](http://images.novusbio.com/fullsize/CEACAM-5-CD66e-Antibody-5C8C4-Immunoprecipitation-NBP2-54625-img0006.jpg)
![Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-74818] - Lysates of mouse embryo tissue loaded at the indicated concentrations. PVDF membrane was probed with 0.2 ug/mL goat anti-P-cadherin polyclonal antibody (AF761) followed by a 1:5000 Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) conjugated to HRP (NBP1-74822). Image using the HRP form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-74818-img0004.jpg "Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-74818] - Lysates of mouse embryo tissue loaded at the indicated concentrations. PVDF membrane was probed with 0.2 ug/mL goat anti-P-cadherin polyclonal antibody (AF761) followed by a 1:5000 Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) conjugated to HRP (NBP1-74822). Image using the HRP form of this antibody.")
![Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-74818] - Lysates of mouse embryo tissue loaded at the indicated concentrations. PVDF membrane was probed with 0.2 ug/mL goat anti-P-cadherin polyclonal antibody (AF761) followed by a 1:5000 donkey anti-goat IgG-HRP (NBP1-74822). Image using the HRP form of Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed).](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-74818-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Goat anti-Guinea Pig IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-74874] - Analysis using the DyLight-594 conjugate of Goat anti-Guinea Pig IgG (H+L) Secondary Antibody (Pre-adsorbed). Mouse brain (corpus striatum). Image from verified customer review.](http://images.novusbio.com/fullsize/Goat-anti-Guinea-Pig-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP1-74874-img0002.jpg "Immunocytochemistry/Immunofluorescence: Goat anti-Guinea Pig IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-74874] - Analysis using the DyLight-594 conjugate of Goat anti-Guinea Pig IgG (H+L) Secondary Antibody (Pre-adsorbed). Mouse brain (corpus striatum). Image from verified customer review.")
![Western Blot: Dynamin Antibody (D5) [NB100-1993] - Analysis of chicken SL29 cell Lysate](http://images.novusbio.com/fullsize/Dynamin-Antibody-D5-Western-Blot-NB100-1993-img0003.jpg "Western Blot: Dynamin Antibody (D5) [NB100-1993] - Analysis of chicken SL29 cell Lysate")
![Western Blot: Dynamin Antibody (D5) [NB100-1993] - Western blot analysis of Dynamin, mAb (D5): Lane 1: MW marker, Lane 2: Mouse Brain, Lane 3: Rat Brain](http://images.novusbio.com/fullsize/Dynamin-Antibody-D5-Western-Blot-NB100-1993-img0001.jpg)