Goat anti-Rabbit IgG (H+L) Secondary Antibody [DyLight 488] (Pre-absorbed)

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Product Details

Summary
Reactivity RbSpecies Glossary
Applications Flow, IM
Clonality
Polyclonal
Host
Goat
Conjugate
DyLight 488
Format
Pre-absorbed
Concentration
LYOPH

Goat anti-Rabbit IgG (H+L) Secondary Antibody [DyLight 488] (Pre-absorbed) Summary

Immunogen
Purified Rabbit IgG (H&L)
Specificity
Based on Immunoelectrophoresis, this antibody reacts with heavy (gamma) chains on rabbit IgG and light chains on all rabbit immunoglobulins. Cross Adsorbed: This antibody was pre-adsorbed to reduce cross-reactivity with proteins of non-related species. Minimal cross-reactivity with bovine, human, mouse IgG serum proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:20-1:2000
  • Immunomicroscopy 1:20-1:2000
Application Notes
This conjugate is suitable for immunomicroscopy and flow cytometry.

Reactivity Notes

Based on IEP, this antibody reacts with heavy (gamma) chains on rabbit IgG and light chains on all rabbit immunoglobulins.

Packaging, Storage & Formulations

Storage
Store at 4C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20C. Avoid repeated freeze-thaw cycles.
Buffer
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free.
Preservative
0.05% Sodium Azide
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 1.1 ml deionized water and let stand 30 minutes at RT to disolve. Centrifuge to remove any particulates.

Notes

Purity > 95% based on SDS-PAGE. Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian. After reconstituted, prepare fresh working dilution daily.

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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