Enzyme-linked immunosorbent assay (ELISA) is an analytic biochemical assay that utilizes enzyme-linked antibodies on a microwell platform to quantitate specific proteins within biological samples. Visual detection is then achieved by adding the enzyme’s substrate, which causes a color-changing reaction to occur. There are three different types of ELISA that are most commonly used: indirect ELISA, sandwich ELISA and competitive ELISA. These assays are extremely useful for high-throughput diagnostic screenings drug testing.
Novus offers a range of support options for ELISA when you buy a product to help you with your ELISA experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77158-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41318-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41250-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77193-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76965-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77127-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77115-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77074-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76918-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76792-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-64803-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-29480-0.025mg | |||
![Western Blot: PIAS1 Antibody [NBP1-77158] - human kidney tissue lysate with PIAS1 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/PIAS1-Antibody-Western-Blot-NBP1-77158-img0001.jpg "Western Blot: PIAS1 Antibody [NBP1-77158] - human kidney tissue lysate with PIAS1 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.")
![Immunohistochemistry: PIAS1 Antibody - BSA Free [NBP1-77158] - Immunohistochemistry of PIAS1 in rat kidney tissue with PIAS1 antibody at 5 u/mL.](http://images.novusbio.com/fullsize/nbp1-77158_rabbit-polyclonal-pias1-antibody-271120241947584.jpg)
![Immunocytochemistry/ Immunofluorescence: PIAS1 Antibody - BSA Free [NBP1-77158] - Immunofluorescence of PIAS1 in rat kidney tissue with PIAS1 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-77158_rabbit-polyclonal-pias1-antibody-271120242074677.jpg)
![Western Blot: MAGEA4 Antibody [NBP2-41318] - A431 cell lysate antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/MAGEA4-Antibody-Western-Blot-NBP2-41318-img0008.jpg "Western Blot: MAGEA4 Antibody [NBP2-41318] - A431 cell lysate antibody at 1 ug/ml.")
![Immunohistochemistry-Paraffin: MAGEA4 Antibody [NBP2-41318] - Human breast tumor with MAGEA4 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/MAGEA4-Antibody-Immunohistochemistry-Paraffin-NBP2-41318-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: MAGEA4 Antibody [NBP2-41318] - HeLa cells with MAGEA4 antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/MAGEA4-Antibody-Immunofluorescence-NBP2-41318-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: MAGEA4 Antibody - BSA Free [NBP2-41318] - Immunofluorescence of MAGEA4 in human breast cancer tissue with MAGEA4 antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/nbp2-41318_rabbit-polyclonal-magea4-antibody-2711202420115559.jpg)
![Immunocytochemistry/ Immunofluorescence: MAGEA4 Antibody - BSA Free [NBP2-41318] - Immunocytochemistry of MAGEA4 in HeLa cells with MAGEA4 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/nbp2-41318_rabbit-polyclonal-magea4-antibody-271120241925152.gif)
![Immunocytochemistry/ Immunofluorescence: MAGEA4 Antibody - BSA Free [NBP2-41318] - Immunofluorescence of MAGEA4 in human breast cancer tissue with MAGEA4 antibody at 20 ug/ml.Green: MAGEA4 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp2-41318_rabbit-polyclonal-magea4-antibody-2711202420312950.gif)
![Western Blot: CXCR3 Antibody [NBP2-41250] - Analysis of CXCR3 in HepG2 lysate with CXCR3 antibody at (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/fullsize/CXCR3-Antibody-Western-Blot-NBP2-41250-img0001.jpg "Western Blot: CXCR3 Antibody [NBP2-41250] - Analysis of CXCR3 in HepG2 lysate with CXCR3 antibody at (A) 1 and (B) 2 ug/ml.")
![Immunocytochemistry/Immunofluorescence: CXCR3 Antibody [NBP2-41250] - Immunocytochemistry of CXCR3 in HepG2 cells with CXCR3 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/CXCR3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-41250-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: CXCR3 Antibody - BSA Free [NBP2-41250] - Immunofluorescence of CXCR3 in HepG2 cells with CXCR3 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41250_rabbit-polyclonal-cxcr3-antibody-2711202420414587.jpg)
![Western Blot: cIAP-1/HIAP-2 Antibody [NBP1-77193] - Western Blot Validation in Human and Rat Tissue Lysates. Loading: 15 ug of lysates per lane. Antibodies: cIAP NBP1-77193 (1 ug/mL ), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/cIAP-1-HIAP-2-Antibody-Western-Blot-NBP1-77193-img0008.jpg "Western Blot: cIAP-1/HIAP-2 Antibody [NBP1-77193] - Western Blot Validation in Human and Rat Tissue Lysates. Loading: 15 ug of lysates per lane. Antibodies: cIAP NBP1-77193 (1 ug/mL ), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Western Blot: cIAP-1/HIAP-2 Antibody [NBP1-77193] - Analysis in human lung lysate with antibody at 1 (lane A), 2 (lane B), and 4 (lane C) ug/mL, respectively.](http://images.novusbio.com/fullsize/cIAP-1-HIAP-2-Antibody-Western-Blot-NBP1-77193-img0005.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: cIAP-1/HIAP-2 Antibody [NBP1-77193] - Independent Antibody Validation (IAV) via Protein Expression Profile in Human, Mouse and Rat Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: cIAP NBP1-77193 (2 ug/mL), cIAP, competitor antibody, (1 ug/mL) and beta-actin NBP1-76692 (1.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/cIAP-1-HIAP-2-Antibody-Western-Blot-NBP1-77193-img0006.jpg)
![Western Blot: cIAP-1/HIAP-2 Antibody [NBP1-77193] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane.Antibodies: cIAP NBP1-77193 (2 ug/mL ), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/cIAP-1-HIAP-2-Antibody-Western-Blot-NBP1-77193-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: cIAP-1/HIAP-2 Antibody [NBP1-77193] - Human Lung cells with cIAP-1/HIAP-2 antibody at 20ug/ml](http://images.novusbio.com/fullsize/cIAP-1-HIAP-2-Antibody-Immunofluorescence-NBP1-77193-img0003.jpg)
![Immunohistochemistry: cIAP-1/HIAP-2 Antibody - BSA Free [NBP1-77193] - Validation of cIAP-1/HIAP-2 in Human Lung Tissue. Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-cIAP-1/HIAP-2 antibody at 10 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77193_rabbit-polyclonal-ciap-1-hiap-2-antibody-2711202419381717.jpg)
![Western Blot: LASS6 Antibody [NBP1-76965] - Rat brain tissue lysate with LASS6 antibody at (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/fullsize/LASS6-Antibody-Western-Blot-NBP1-76965-img0002.jpg "Western Blot: LASS6 Antibody [NBP1-76965] - Rat brain tissue lysate with LASS6 antibody at (A) 1 and (B) 2 ug/ml.")
![<b>Genetic Strategies Validation. </b>Western Blot: LASS6 Antibody [NBP1-76965] - CerS6 (LASS6)-dependent C16-ceramide generation mediates stress response to serum starvation. Serum withdrawal elevates CerS6 in cultured cells with concomitant increase of p53 and p21 while CerS6 siRNA silencing prevents the p53 and p21 accumulation in response to serum withdrawal. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41467-018-06650-y) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LASS6-Antibody-Knockdown-Validated-NBP1-76965-img0004.jpg)
![Immunohistochemistry: LASS6 Antibody - BSA Free [NBP1-76965] - Immunohistochemistry of LASS6 in mouse brain tissue with LASS6 antibody at 2.5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76965_rabbit-polyclonal-lass6-antibody-271120242013120.jpg)
![Immunocytochemistry/ Immunofluorescence: LASS6 Antibody - BSA Free [NBP1-76965] - Immunofluorescence of LASS6 in Mouse Brain cells with LASS6 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76965_rabbit-polyclonal-lass6-antibody-2711202419592022.jpg)
![Western Blot: DCLK1 Antibody [NBP1-77127] - Analysis of DCLK1 expression in rat brain tissue lysate with DCLK1 antibody at (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/fullsize/DCLK1-Antibody-Western-Blot-NBP1-77127-img0006.jpg "Western Blot: DCLK1 Antibody [NBP1-77127] - Analysis of DCLK1 expression in rat brain tissue lysate with DCLK1 antibody at (A) 1 and (B) 2 ug/ml.")
![Immunohistochemistry: DCLK1 Antibody [NBP1-77127] - Staining of DCLK1 in mouse brain tissue with DCLK1 Antibodyat 5 ug/mL.](http://images.novusbio.com/fullsize/DCLK1-Antibody-Immunohistochemistry-NBP1-77127-img0004.jpg)
![Western Blot: DCLK1 Antibody [NBP1-77127] - Human brain tissue lysate with DCAMILK1 antibody at (A) 0.5 and (B) 1 ug/mL.](http://images.novusbio.com/fullsize/DCLK1-Antibody-Western-Blot-NBP1-77127-img0001.jpg)
![Immunohistochemistry: DCLK1 Antibody - BSA Free [NBP1-77127] - Immunohistochemistry of DLCK1 in rat brain tissue with DLCK1 antibody at 2.5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77127_rabbit-polyclonal-dclk1-antibody-271120241958465.jpg)
![Immunocytochemistry/ Immunofluorescence: DCLK1 Antibody - BSA Free [NBP1-77127] - Immunofluorescence of DCLK1 in mouse brain tissue with DCLK1 Antibody at 20 ug/mL.Green: DCLK1 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-77127_rabbit-polyclonal-dclk1-antibody-2711202420432326.gif)
![Western Blot: GATA-3 Antibody [NBP1-77115] - Analysis in K562 cell lysate with antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/GATA-3-Antibody-Western-Blot-NBP1-77115-img0010.jpg "Western Blot: GATA-3 Antibody [NBP1-77115] - Analysis in K562 cell lysate with antibody at 1 ug/mL.")
![Immunocytochemistry/ Immunofluorescence: GATA-3 Antibody - BSA Free [NBP1-77115] - Immunofluorescence of GATA-3 in Rat Brain tissue with GATA-3 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-77115_rabbit-polyclonal-gata-3-antibody-2711202420212392.jpg)
![Immunohistochemistry: GATA-3 Antibody - BSA Free [NBP1-77115] - Immunohistochemistry of GATA-3 in mouse brain tissue with GATA-3 antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/nbp1-77115_rabbit-polyclonal-gata-3-antibody-2711202419463470.gif)
![Immunohistochemistry: GATA-3 Antibody - BSA Free [NBP1-77115] - Immunohistochemistry of GATA-3 in rat brain tissue with GATA-3 antibody at 2.5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77115_rabbit-polyclonal-gata-3-antibody-2711202419172230.jpg)
![Immunocytochemistry/ Immunofluorescence: GATA-3 Antibody - BSA Free [NBP1-77115] - Immunofluorescence of GATA-3 in mouse brain tissue with GATA-3 Antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77115_rabbit-polyclonal-gata-3-antibody-271120242042572.gif)
![Western Blot: JMJD5 Antibody [NBP1-77074] - Human liver tissue lysate with JMJD5 antibody at (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/fullsize/JMJD5-Antibody-Western-Blot-NBP1-77074-img0002.jpg "Western Blot: JMJD5 Antibody [NBP1-77074] - Human liver tissue lysate with JMJD5 antibody at (A) 1 and (B) 2 ug/ml.")
![Immunohistochemistry-Paraffin: JMJD5 Antibody [NBP1-77074] - Rat liver tissue with JMJD5 antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/JMJD5-Antibody-Immunohistochemistry-Paraffin-NBP1-77074-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: JMJD5 Antibody - BSA Free [NBP1-77074] - Immunofluorescence of JMJD5 in Rat Liver cells with JMJD5 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77074_rabbit-polyclonal-jmjd5-antibody-2711202419333429.jpg)
![Western Blot: SLC22A17 Antibody [NBP1-76918] - Analysis in SK-N-SH lysate with antibody at 0.5 ug/mL in (A) the presence and (B) the absence of blocking peptide.](http://images.novusbio.com/fullsize/SLC22A17-Antibody-Western-Blot-NBP1-76918-img0005.jpg "Western Blot: SLC22A17 Antibody [NBP1-76918] - Analysis in SK-N-SH lysate with antibody at 0.5 ug/mL in (A) the presence and (B) the absence of blocking peptide.")
![Immunocytochemistry/ Immunofluorescence: SLC22A17 Antibody - BSA Free [NBP1-76918] - Immunofluorescence of SLC22A17 in rat kidney tissue tissue with SLC22A17 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76918_rabbit-polyclonal-slc22a17-antibody-271120242013237.jpg)
![Immunocytochemistry/ Immunofluorescence: SLC22A17 Antibody - BSA Free [NBP1-76918] - Immunofluorescence of SLC22A17 in mouse kidney tissue with SLC22A17 antibody at 20 ug/ml.Green: Slc22A17 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-76918_rabbit-polyclonal-slc22a17-antibody-271120241928813.gif)
![Immunohistochemistry: SLC22A17 Antibody - BSA Free [NBP1-76918] - Immunohistochemistry of SLC22A17 in mouse kidney tissue with SLC22A17 antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/nbp1-76918_rabbit-polyclonal-slc22a17-antibody-2711202419293816.gif)
![Western Blot: Presenilin-1 Antibody [NBP1-76792] - Human brain lysate with presenilin1 antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.](http://images.novusbio.com/fullsize/Presenilin-1-Antibody-Western-Blot-NBP1-76792-img0002.jpg "Western Blot: Presenilin-1 Antibody [NBP1-76792] - Human brain lysate with presenilin1 antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.")
![Immunocytochemistry/Immunofluorescence: Presenilin-1 Antibody [NBP1-76792] - Cell surface staining of VDR (green) on live neurons followed by fixation and immunofluorescence labeling of target protein (100x) Presenilin-1. Overlay images indicate the possible co-localization of limited co-localization of VDR/ Presenilin-1 on the neuronal plasma membrane. Image collected and cropped by CiteAb from the following publication (//journals.plos.org/plosone/article?id=10.1371/journal.pone.0188605) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Presenilin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76792-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: Presenilin-1 Antibody - BSA Free [NBP1-76792] - Immunofluorescence of Presenilin-1 in Human Brain cells with Presenilin-1 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76792_rabbit-polyclonal-presenilin-1-antibody-2711202419201530.jpg)
![Immunohistochemistry: Presenilin-1 Antibody - BSA Free [NBP1-76792] - Immunohistochemistry of Presenilin-1 in human brain tissue with Presenilin-1 antibody at 2.5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76792_rabbit-polyclonal-presenilin-1-antibody-271120242092064.jpg)
![Immunohistochemistry-Paraffin: Myeloperoxidase/MPO Antibody (2C7) [NB100-64803] - Mouse anti Human Myeloperoxidase.](http://images.novusbio.com/fullsize/Myeloperoxidase-MPO-Antibody-2C7-Immunohistochemistry-Paraffin-NB100-64803-img0002.jpg "Immunohistochemistry-Paraffin: Myeloperoxidase/MPO Antibody (2C7) [NB100-64803] - Mouse anti Human Myeloperoxidase.")
![Flow Cytometry: Myeloperoxidase/MPO Antibody (2C7) [NB100-64803] - An intracellular stain was performed on Jurkat cells with Myeloperoxidase antibody (2C7) NB100-64803PCP (blue) and a matched isotype control NBP2-27287PCP (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Peridinin-Chlorophyll-Protein complex.](http://images.novusbio.com/fullsize/Myeloperoxidase-MPO-Antibody-2C7-Flow-Cytometry-NB100-64803-img0005.jpg)
![Flow Cytometry: Myeloperoxidase/MPO Antibody (2C7) [NB100-64803] - Staining of human peripheral blood granulocytes with Mouse anti Human myeloperoxidase following permeabilisation with Leucoperm.](http://images.novusbio.com/fullsize/Myeloperoxidase-MPO-Antibody-2C7-Flow-Cytometry-NB100-64803-img0003.jpg)
![Flow Cytometry: Myeloperoxidase/MPO Antibody (2C7) [NB100-64803] - Analysis using the FITC conjugate of NB100-64803. Staining of permeabilised human peripheral blood granulocytes.](http://images.novusbio.com/fullsize/Myeloperoxidase-MPO-Antibody-2C7-Flow-Cytometry-NB100-64803-img0004.jpg)
![Immunohistochemistry-Paraffin: CXCL12/SDF-1 alpha Antibody [NBP2-29480] - Formalin fixed paraffin embedded tissue section of human colorectal carcinoma stained using SDF-1 alpha antibody (NBP2-29480) with DAB detection. Sodium citrate buffer (pH6.0) based heat mediated antigen retrieval was employed in this IHC-P validation.](http://images.novusbio.com/fullsize/CXCL12-SDF-1-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-29480-img0007.jpg "Immunohistochemistry-Paraffin: CXCL12/SDF-1 alpha Antibody [NBP2-29480] - Formalin fixed paraffin embedded tissue section of human colorectal carcinoma stained using SDF-1 alpha antibody (NBP2-29480) with DAB detection. Sodium citrate buffer (pH6.0) based heat mediated antigen retrieval was employed in this IHC-P validation.")
![Immunohistochemistry-Paraffin: CXCL12/SDF-1 alpha Antibody [NBP2-29480] - Formalin fixed paraffin embedded tissue section of human colorectal carcinoma stained using SDF-1 alpha antibody (NBP2-29480) with DAB detection. Sodium citrate buffer (pH6.0) based heat mediated antigen retrieval was employed in this IHC-P validation.](http://images.novusbio.com/fullsize/CXCL12-SDF-1-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-29480-img0006.jpg)