200-LA)." class="big_lightbox"> 200-LA)." alt="Recombinant Human IL-1ra/IL-1F3 (Catalog # 280-RA) inhibits IL-1a-dependent cell proliferation of the D10.G4.1 mouse helper T cell line. The ED₅₀ for this effect is 5-40 ng/mL in the presence of 50 pg/mL of Recombinant Human IL-1a/IL-1F1 (Catalog # 200-LA)." class="big_thumb" />

HAF017). A specific band was detected for IL-1ra/IL-1F3 at approximately 18-20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). A specific band was detected for IL-1ra/IL-1F3 at approximately 18-20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of RT-4 human bladder carcinoma cell line and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human IL-1ra/IL-1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-280-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL-1ra/IL-1F3 at approximately 18-20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to keratinocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to keratinocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox"> HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

280-RA) inhibits Recombinant Human IL-1a/IL-1F1 (Catalog # 200-LA) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Inhibition of Recombinant Human IL-1a/IL-1F1 (50 pg/mL) activity elicited by Recombinant Human IL-1ra/IL-1F3 (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-1ra/IL-1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-280-NA). The ND₅₀ is typically ≤ 4 µg/mL." class="big_lightbox"> 280-RA) inhibits Recombinant Human IL-1a/IL-1F1 (Catalog # 200-LA) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Inhibition of Recombinant Human IL-1a/IL-1F1 (50 pg/mL) activity elicited by Recombinant Human IL-1ra/IL-1F3 (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-1ra/IL-1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-280-NA). The ND₅₀ is typically ≤ 4 µg/mL." class="big_thumb" />

280-RA) was serially diluted and captured by Mouse Anti-Human IL‑1ra/IL‑1F3 Monoclonal Antibody (Catalog # MAB280) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑1ra/IL‑1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-280-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 280-RA) was serially diluted and captured by Mouse Anti-Human IL‑1ra/IL‑1F3 Monoclonal Antibody (Catalog # MAB280) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑1ra/IL‑1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-280-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

480-RM) inhibits Recombinant Human IL-1a/IL-1F1 (Catalog # 200-LA) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL-1a/IL-1F1 (50 pg/mL) activity elicited by Recombinant Mouse IL-1ra/IL-1F3 (150 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-1ra/IL-1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-480-NA). The ND₅₀ is typically 1-5 µg/mL." class="big_lightbox"> 480-RM) inhibits Recombinant Human IL-1a/IL-1F1 (Catalog # 200-LA) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL-1a/IL-1F1 (50 pg/mL) activity elicited by Recombinant Mouse IL-1ra/IL-1F3 (150 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-1ra/IL-1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-480-NA). The ND₅₀ is typically 1-5 µg/mL." alt="Recombinant Mouse IL-1ra/IL-1F3 (Catalog # 480-RM) inhibits Recombinant Human IL-1a/IL-1F1 (Catalog # 200-LA) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL-1a/IL-1F1 (50 pg/mL) activity elicited by Recombinant Mouse IL-1ra/IL-1F3 (150 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-1ra/IL-1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-480-NA). The ND₅₀ is typically 1-5 µg/mL." class="big_thumb" />

480-RM) was serially diluted and captured by Goat Anti-Mouse IL‑1ra/IL‑1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-480-NA) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑1ra/IL‑1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-480-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 480-RM) was serially diluted and captured by Goat Anti-Mouse IL‑1ra/IL‑1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-480-NA) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑1ra/IL‑1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-480-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

480-RM) was serially diluted and captured by Goat Anti-Mouse IL‑1ra/IL‑1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-480-NA) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑1ra/IL‑1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-480-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 480-RM) was serially diluted and captured by Goat Anti-Mouse IL‑1ra/IL‑1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-480-NA) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑1ra/IL‑1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-480-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

280-RA) inhibits Recombinant Human IL-1a/IL-1F1 (Catalog # 200-LA) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL-1a/IL-1F1 (50 pg/mL) activity elicited by Recombinant Human IL-1ra/IL-1F3 (50 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-1ra/IL-1F3 Monoclonal Antibody (Catalog # MAB280). The ND₅₀ is typically 10-20 µg/mL." class="big_lightbox"> 280-RA) inhibits Recombinant Human IL-1a/IL-1F1 (Catalog # 200-LA) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL-1a/IL-1F1 (50 pg/mL) activity elicited by Recombinant Human IL-1ra/IL-1F3 (50 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-1ra/IL-1F3 Monoclonal Antibody (Catalog # MAB280). The ND₅₀ is typically 10-20 µg/mL." alt="Recombinant Human IL-1ra/IL-1F3 (Catalog # 280-RA) inhibits Recombinant Human IL-1a/IL-1F1 (Catalog # 200-LA) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL-1a/IL-1F1 (50 pg/mL) activity elicited by Recombinant Human IL-1ra/IL-1F3 (50 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-1ra/IL-1F3 Monoclonal Antibody (Catalog # MAB280). The ND₅₀ is typically 10-20 µg/mL." class="big_thumb" />

280-RA) was serially diluted and captured by Mouse Anti-Human IL‑1ra/IL‑1F3 Monoclonal Antibody (Catalog # MAB280) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑1ra/IL‑1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-280-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 280-RA) was serially diluted and captured by Mouse Anti-Human IL‑1ra/IL‑1F3 Monoclonal Antibody (Catalog # MAB280) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑1ra/IL‑1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-280-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />