HAF019). A specific band was detected for FGF-21 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF019). A specific band was detected for FGF-21 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse spleen tissue and rat spleen tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse FGF-21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3057) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for FGF-21 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

MAB25374 coated on a Clear Polystyrene Microplate DY990. Goat Anti-Human FGF‑21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2539) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP DY998 followed by Substrate Solution DY999 and stopping the enzymatic reaction with Stop Solution DY994." class="big_lightbox"> MAB25374 coated on a Clear Polystyrene Microplate DY990. Goat Anti-Human FGF‑21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2539) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP DY998 followed by Substrate Solution DY999 and stopping the enzymatic reaction with Stop Solution DY994." alt="Recombinant Human FGF‑21 protein was serially diluted 2-fold and captured by Mouse Anti-Human FGF‑21 Monoclonal Antibody MAB25374 coated on a Clear Polystyrene Microplate DY990. Goat Anti-Human FGF‑21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2539) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP DY998 followed by Substrate Solution DY999 and stopping the enzymatic reaction with Stop Solution DY994." class="big_thumb" />

2539-FG) was serially diluted and captured by Mouse Anti-Human FGF‑21 Monoclonal Antibody (Catalog # MAB25374) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human FGF‑21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2539) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 2539-FG) was serially diluted and captured by Mouse Anti-Human FGF‑21 Monoclonal Antibody (Catalog # MAB25374) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human FGF‑21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2539) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />