| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-582SS | |||
| Azide and BSA Free | NBP2-80959 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2215SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74392 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84827-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-81051-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61764-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84826-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43926 | |||
| BSA Free | NBP2-34478-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70687 | |||
![Western Blot: SREBP1 Antibody (2A4) [NB600-582] - Hepatic SREBP1c and SREBP2 protein abundance in C57BL/6 mice assigned to a high-fat (HF) or a high-fat/exercise (HF + EX) group for 8 weeks. Cytosolic and nuclear SREBP1c abundance. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/jl/2013/908048/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SREBP1-Antibody-2A4-Western-Blot-NB600-582-img0004.jpg "Western Blot: SREBP1 Antibody (2A4) [NB600-582] - Hepatic SREBP1c and SREBP2 protein abundance in C57BL/6 mice assigned to a high-fat (HF) or a high-fat/exercise (HF + EX) group for 8 weeks. Cytosolic and nuclear SREBP1c abundance. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/jl/2013/908048/), licensed under a CC-BY license.")
![Western Blot: SREBP1 Antibody (2A4) [NB600-582] - Analysis of whole cell lysate from HeLa cells showing a single specific band for the expression of SREBP1 precursor protein (~120 kDa).](http://images.novusbio.com/fullsize/SREBP1-Antibody-2A4-Western-Blot-NB600-582-img0002.jpg)
![Simple Western: SREBP1 Antibody (2A4) [NB600-582] - Lane view shows a specific band for SREBP1 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/SREBP1-Antibody-2A4-Simple-Western-NB600-582-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: SREBP1 Antibody (2A4) [NB600-582] - KO and OE of SREBP1 influenced lipid metabolism in Bel-7402 cells. The protein level of lipid-associated genes in SREBP1-KO, SREBP1-OE, and WT Bel-7402 cells. Data were expressed as mean -/+ standard error of the mean. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. WT group, respectively.](http://images.novusbio.com/fullsize/SREBP1-Antibody-2A4-Knockout-Validated-NB600-582-img0005.jpg)
![Western Blot: SREBP1 Antibody (2A4) - BSA Free [NB600-582] - Effect of GEN on the expression of Nrf2‐related signalling proteins in OA (660 μmol/L) & PA (330 μmol/L)‐induced HepG2 cells. GEN was added into HepG2 cells prior 1 h to stimulation of OA & PA for 18 h. A‐C, Protein expression of Nrf2, PPAR alpha , PPAR gamma in nucleus & HO‐1 in cytoplasm was detected by Western blot. (D & E) Protein expression of P‐ACC, ACC, P‐AKT, AKT, P‐AMPK alpha , AMPK alpha , P‐AMPK beta & AMPK beta was detected by Western blot. F‐H, Protein expression of PI3K, P‐mTORC, mTORC, P‐S6K, S6K, P‐S6, S6, SREBP‐1c & HMGB1 was detected by Western blot. The similar results were collected from three dependent experiments. All data were expressed by mean ± SEM (n = 5 in each group). ##P < .01 vs Control Group; *P < .05 & **P < .01 vs OA & PA Group Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32293113), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-582_mouse-monoclonal-srebp1-antibody-2a4-31020241534375.jpg)
![Western Blot: SREBP1 Antibody (2A4) - BSA Free [NB600-582] - KO & OE of SREBP1 influenced lipid metabolism in Bel-7402 cells. (A) Total cellular TG content of SREBP1-KO, SREBP1-OE, & WT Bel-7402 cells. (B) HPLC-MS determination of the SFAs to MUFAs ratios (palmitic acid to palmitoleic acid, 16:0 to 16:1) (stearic acid to oleic acid, 18:0 to 18:1) as well as the content of 16:0, 16:1, 18:0, & 18:1 in SREBP1-KO, SREBP1-OE, & WT Bel-7402 cells. (C) The transcript level of lipid-associated genes in SREBP1-KO, SREBP1-OE, & WT Bel-7402 cells. (D & E) The protein level of lipid-associated genes in SREBP1-KO, SREBP1-OE, & WT Bel-7402 cells. Data were expressed as mean ± standard error of the mean. *P < 0.05, **P < 0.01, & ***P < 0.001 vs. WT group, respectively. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31297058), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-582_mouse-monoclonal-srebp1-antibody-2a4-310202415384185.jpg)
![Western Blot: SREBP1 Antibody (2A4) - BSA Free [NB600-582] - Construction of knockout (KO) & overexpression (OE) cell lines. (A) The sequencing results of KO positive clone cells; (B) The messenger RNA (mRNA) level of SREBP1 in wild type (WT) & OE Bel-7402 cells. Data were expressed as mean ± standard error of the mean. ***P < 0.001 vs. WT group; (C & D) The protein level of SREBP1 in WT, OE, & KO Bel-7402 cells. Data were expressed as mean ± standard error of the mean. ***P < 0.001 vs. WT group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31297058), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-582_mouse-monoclonal-srebp1-antibody-2a4-31020241539748.jpg)
![Western Blot: SREBP1 Antibody (2A4) - BSA Free [NB600-582] - p75NTR stimulation activated SREBP1 & SREBP2 in Huh7 hepatocyte cells.Human Huh7 cells were stimulated with 10 ng/ml pro-NGF or 50 ng/ml NGF for 16 h followed by immunoblotting using antibodies to detect the presence of cleaved/activated SREBP1 & SREBP2. a SREBP1, immunoblots & b quantification. Values are mean ± SD, n = 3. **P < 0.01 & *p < 0.05 for treated vs. control cells. c, d SREBP2. Cells were treated with 10 ng/ml pro-NGF in the absence or presence of 0.5 μM PF429242 (PF), which is a selective inhibitor of the Site 1 protease (S1P). c Immunoblots & d quantification. PF decreased the amount of processed SREBP2 in these cells; however, addition of pro-NGF increased the relative level of cleaved SREBP2. Values are mean ± SD, n = 3. *p < 0.05 for treated vs. the corresponding control Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31296846), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-582_mouse-monoclonal-srebp1-antibody-2a4-310202416161836.jpg)
![Western Blot: SREBP1 Antibody (2A4) - BSA Free [NB600-582] - p75NTR stimulation activated SREBP1 & SREBP2 in Huh7 hepatocyte cells.Human Huh7 cells were stimulated with 10 ng/ml pro-NGF or 50 ng/ml NGF for 16 h followed by immunoblotting using antibodies to detect the presence of cleaved/activated SREBP1 & SREBP2. a SREBP1, immunoblots & b quantification. Values are mean ± SD, n = 3. **P < 0.01 & *p < 0.05 for treated vs. control cells. c, d SREBP2. Cells were treated with 10 ng/ml pro-NGF in the absence or presence of 0.5 μM PF429242 (PF), which is a selective inhibitor of the Site 1 protease (S1P). c Immunoblots & d quantification. PF decreased the amount of processed SREBP2 in these cells; however, addition of pro-NGF increased the relative level of cleaved SREBP2. Values are mean ± SD, n = 3. *p < 0.05 for treated vs. the corresponding control Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31296846), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-582_mouse-monoclonal-srebp1-antibody-2a4-310202416175210.jpg)
![Western Blot: SREBP1 Antibody (2A4) - BSA Free [NB600-582] - Effect of GEN on the protein expression of Nrf2, ACC & mTORC signalling pathways in tyloxapol‐induced mice. GEN (50, 75, 100 mg/kg) was administered to WT or Nrf2−/− mice prior 1 h to stimulation of tyloxapol (500 mg/kg) for 18 h. A‐C, Protein expression of Nrf2, PPAR alpha , PPAR gamma in nucleus & HO‐1 in cytoplasm of WT mice was detected by Western blot. (D & E) Protein expression of Nrf2, PPAR alpha & PPAR gamma in Nrf2−/− mice was detected by Western blot. F‐H, Protein expression of P‐ACC, ACC, P‐AKT, AKT, P‐AMPK alpha , AMPK alpha , P‐AMPK beta , AMPK beta in WT & Nrf2−/− mice was detected by Western blot. I‐M, Protein expression of PI3K, P‐mTORC, mTORC, P‐S6K, S6K, P‐S6, S6, SREBP‐1c & HMGB1 WT & Nrf2−/− mice was detected by Western blot. The similar results were collected from three dependent experiments. All data were expressed by mean ± SEM (n = 5 in each group). ##P < .01 vs Control group; *P < .05 & **P < .01 vs Tyloxapol group Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32293113), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-582_mouse-monoclonal-srebp1-antibody-2a4-310202416171460.jpg)
![Simple Western: SREBP1 Antibody [NB100-2215] - Image shows a specific band for SREBP1 in 1 mg/mL of HeLa cell lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/SREBP1-Antibody-Simple-Western-NB100-2215-img0007.jpg "Simple Western: SREBP1 Antibody [NB100-2215] - Image shows a specific band for SREBP1 in 1 mg/mL of HeLa cell lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Immunocytochemistry/Immunofluorescence: SREBP1 Antibody [NB100-2215] - CDC2 associates with SPDY at ERES. (A-C) 0 h GV stage oocyte labeled for CDC2 (A, green), DNA (A, blue), and SPDY (B, red). Both CDC2 and SPDY localize to the same cortical domain (C). Note that the center of this oocyte is dented (the area that contains the arrow) causing the structure to appear in the middle of the oocyte, whereas it is located in the cortex. Images are Z-projections of 2 (A-C); scale bars represent 20 um in C and 5 um in C'. Arrows indicate the region of the oocyte that is shown enlarged in the insets (B'-C'). Arrowheads denote the position of the GV. Image collected and cropped by CiteAb from the following publication (//bmcdevbiol.biomedcentral.com/articles/10.1186/1471-213X-9-8) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SREBP1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2215-img0011.jpg)
![Western Blot: SREBP1 Antibody [NB100-2215] - MiR-181a overexpression impairs and miR-181a inhibition improves glucose and lipid homeostasis in HepG2 cellsHepG2 cells were divided into 4 groups as follows: a control group, mimics group (HepG2 cells transfected with 10 nM mimics), NEFA group (treated with 1. 2 mM NEFA), and miR-181a + NEFA group (transfected with 50 nM miR-181a inhibitors and then treated with 1. 2 mM NEFA). It was followed with or without 100 nM insulin. Immunoblot analysis (left) and quantification (right) of SREBP-1c and PPARI+- in HepG2 cells. Image collected and cropped by Citeab from the following publication (Upregulation of miR-181a impairs hepatic glucose and lipid homeostasis. Oncotarget (2017)) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SREBP1-Antibody-Western-Blot-NB100-2215-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: SREBP1 Antibody [NB100-2215] - SREBP1 antibody was tested in HeLa cells with DyLight488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Nuclear and punctate vesicle staining was observed.](http://images.novusbio.com/fullsize/SREBP1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2215-img0006.jpg)
![Immunohistochemistry-Paraffin: SREBP1 Antibody [NB100-2215] - nalysis of FFPE tissue section of mouse liver with SREBP1 antibody at 1:200. The antibody generated an expected cytoplasmic-nuclear immunostaining of SREBP1 protein in the hepatocytes.](http://images.novusbio.com/fullsize/SREBP1-Antibody-Immunohistochemistry-Paraffin-NB100-2215-img0008.jpg)
![Western Blot: SREBP1 Antibody [NB100-2215] - Detection of SREBP1. Lane 1: human liver. Lane 2: mouse liver.](http://images.novusbio.com/fullsize/SREBP1-Antibody-Western-Blot-NB100-2215-img0004.jpg)
![Western Blot: SREBP1 Antibody - BSA Free [NB100-2215] - MiR-181a overexpression impairs & miR-181a inhibition improves glucose & lipid homeostasis in HepG2 cellsHepG2 cells were divided into 4 groups as follows: a control group, mimics group (HepG2 cells transfected with 10 nM mimics), NEFA group (treated with 1.2 mM NEFA), & miR-181a + NEFA group (transfected with 50 nM miR-181a inhibitors & then treated with 1.2 mM NEFA). B was followed with or without 100 nM insulin. (A) Immunoblot analysis (top) & quantification (bottom) of SIRT1, PGC-1 alpha & acetylated PGC-1 alpha in HepG2 cells. (B) Immunoblotting analysis (top) & quantification (bottom) of insulin-stimulated phosphorylation of AKT in HepG2 cells. (C) Glucose concentration in medium. (D) The glycogen content in HepG2 cells. (E) The mRNA expression levels of G6Pase & PEPCK. (F) Immunoblot analysis (left) & quantification (right) of SREBP-1c & PPAR alpha in HepG2 cells. (G) TG content in HepG2 cells. *P < 0.05, **P < 0.01. All experiments were repeated at least three times & representative results are shown. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29207650), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2215_rabbit-polyclonal-srebp1-antibody-31020241682194.jpg)
![Western Blot: SREBP1 Antibody - BSA Free [NB100-2215] - Effect of restoration of macrophage A2bAR on liver.Liver was collected from WT, A2bAR KO, & CD68-Tg mice after 16 weeks of HFD as described in the methods. Relative mRNA expression was determined using the delta delta CT method with normalization to 18s rRNA. A. mRNA expression of TNF-alpha in liver. A2bAR KO (n = 5) vs WT (n = 7) p-value = 0.0271; A2bAR KO vs CD68-Tg (n = 6) p-value = 0.0210. B. mRNA expression of IL-6 in liver. A2bAR KO (n = 8) vs WT (n = 7) p-value = 0.0159; A2bAR KO vs CD68-Tg (n = 8) p-value = 0.0080. C. mRNA expression of IRS-2 in liver. CD68-Tg (n = 7) vs A2bAR KO (n = 8) p-value = 0.0300. D. Western blot analysis of liver; one representative (of 3 sets) WT, CD68-Tg & A2bAR KO group shown at 15 minutes post-insulin injection, following 16 weeks of HFD. Levels of mature SREBP-1 (68 kDa), & IRS-2 (185 kDa), were probed by Western blot analysis, using beta -actin (43 kDa) as loading control. Quantification of Western Blot results was performed with Image J software (//rsb.info.nih.gov/ij/) with normalization to beta -actin. WT to A2bAR KO: IRS-2 p-value = 0.0092, SREBP-1 p-value = 0.0154; CD68-Tg to A2bAR KO: IRS-2 p-value = 0.0247, SREBP-1 p-value = 0.0170. Data are averages ± SD. *Student two-tail t-test assuming equal variance was found significant only when p-value <0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24892847), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2215_rabbit-polyclonal-srebp1-antibody-31020241682175.jpg)
![Western Blot: SREBP1 Antibody - BSA Free [NB100-2215] - MiR-181a overexpression or SIRT1 knockdown impairs glucose & lipid metabolism in vivo(Aa & Ab) Immunoblotting analysis (left) & quantification (right) of SIRT1, PGC-1 alpha & acetylated PGC-1 alpha in the liver of mice injected with Ago-181a or Ago-NC or Ad-shRNA NC or Ad-shRNA SIRT1. (B) Glucose tolerance test of mice injected with Ago-181a (n = 7) or Ago-NC (n = 7) or Ad-shRNA NC (n = 6) or Ad-shRNA SIRT1 (n = 6). (C) Insulin tolerance test of mice injected with Ago-181a (n = 7) or Ago-NC (n = 7) or Ad-shRNA NC (n = 6) or Ad-shRNA SIRT1 (n = 6). (D) Representative images of PAS staining (original magnification × 20) of liver sections from mice injected with Ago-181a or Ago-NC or Ad-shRNA NC or Ad-shRNA SIRT1. (Ea & Eb) Immunoblotting analysis (top) & quantification (bottom) of insulin-stimulated phosphorylation of IR, AKT & GSK3 beta in liver of mice injected with Ago-181a or Ago-NC or Ad-shRNA NC or Ad-shRNA SIRT1. (F) Representative images of H&E staining (left, original magnification × 20) & Oil-red staining (right, original magnification × 20) of liver sections from mice injected with Ago-181a or Ago-NC or Ad-shRNA NC or Ad-shRNA SIRT1. (Ga & Gb) Immunoblotting analysis (top) & quantification (bottom) of SREBP-1c & PPAR alpha in the liver of mice injected with Ago-181a or Ago-NC or Ad-shRNA NC or Ad-shRNA SIRT1. *P < 0.05, **P < 0.01. All experiments were repeated at least three times & representative results are shown. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29207650), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2215_rabbit-polyclonal-srebp1-antibody-31020241553209.jpg)
![Western Blot: SREBP1 Antibody - BSA Free [NB100-2215] - MiR-181a overexpression impairs & miR-181a inhibition improves glucose & lipid metabolism in vitro(A, B, C, D & E) Hepatocytes were transfected with 10 nM miR-181a mimics or negative controls in the absence or presence of 100 nM insulin. (A) Immunoblot analysis (left) & quantification (right) of insulin-stimulated phosphorylation of IR, Akt & GSK3 beta in hepatocytes. (B) The mRNA expression levels of G6Pase & PEPCK in hepatocytes. (C) Glucose concentration in medium. (D) Immunoblot analysis (left) & quantification (right) of SREBP-1c & PPAR alpha expression in hepatocytes. (E) TG content in hepatocytes. (F, G, H, I & J) Hepatocytes were transfected with 50 nM miR-181a inhibitors or negative controls in the absence or presence of 100 nM insulin. (F) Immunoblot analysis (left) & quantification (right) of insulin-stimulated phosphorylation of IR, Akt & GSK3 beta in hepatocytes. (G) The expression levels of G6Pase & PEPCK in hepatocytes. (H) Glucose concentration in medium. (I) Immunoblot analysis (left) & quantification (right) of SREBP-1c & PPAR alpha expression in hepatocytes. (J) TG content in hepatocytes. *P < 0.05, **P < 0.01. All experiments were repeated at least three times & representative results are shown. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29207650), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2215_rabbit-polyclonal-srebp1-antibody-310202415535169.jpg)
![Western Blot: SREBP1 Antibody - BSA Free [NB100-2215] - Effect of restoration of macrophage A2bAR on adipose tissue.Visceral (epididymal) adipose tissue was collected from WT, A2bAR KO, & CD68-Tg mice after 16 weeks of HFD as described in the methods. A-C,E. Relative mRNA expression was determined using the delta delta CT method with normalization to 18s rRNA. A. mRNA expression of TNF-alpha in visceral fat. A2bAR KO (n = 7) vs CD68-Tg (n = 9) p-value = 0.0345. B. mRNA expression of IL-6 in visceral fat. A2bAR KO (n = 7) vs WT (n = 7) p-value = 0.0073; A2bAR KO vs CD68-Tg (n = 7) p-value = 0.0141. C. mRNA expression of MCP1 in visceral fat. A2bAR KO (n = 7) vs CD68-Tg (n = 9) p-value = 0.0232. D. Visceral adipose tissue from WT, A2bAR KO, & CD68-Tg mice after 16 weeks of HFD was fixed in 4% paraformaldehyde & paraffin-embedded. Sections immunostained with the macrophage marker F4/80. Representative sections for each genotype at a magnification of 200x & 400x. Arrows point to crown-like structures. E. mRNA expression of IRS-2 in visceral fat. CD68-Tg (n = 8) vs A2bAR KO (n = 8) p-value = 0.0115, WT (n = 6) vs A2bAR KO p-value = 0.0358. F. Western blot analysis of visceral fat; one representative (of 3 sets) WT, CD68-Tg & A2bAR KO group shown at 15 minutes post-insulin injection, following 16 weeks of HFD. WT to A2bAR KO: IRS-2 p-value = 0.0411, SREBP-1 p-value = 0.0103. CD68-Tg to A2bAR KO: IRS-2 p-value = 0.0305, SREBP-1 p-value = 0.0459. Data are averages ± SD. *Student two-tail t-test assuming equal variance was found significant only when p-value <0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24892847), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2215_rabbit-polyclonal-srebp1-antibody-31020241682189.jpg)
![Western Blot: SREBP1 Antibody - BSA Free [NB100-2215] - SIRT1 knockdown impairs & SIRT1 overexpression improves glucose & lipid metabolism in hepatocytes(A) Immunoblot analysis (left) & quantification (right) of insulin-stimulated phosphorylation of IR, Akt & GSK3 beta protein levels in hepatocytes. Hepatocytes were transfected with SIRT1 siRNA with or without 100 nM insulin. (B) Immunoblot analysis (left) & quantification (right) of SREBP-1c & PPAR alpha protein levels in hepatocytes. Hepatocytes were transfected with SIRT1 siRNA. (C) Immunoblot analysis (left) & quantification (right) of SIRT1, PGC-1 alpha & acetylated PGC-1 alpha in hepatocytes. Hepatocytes were infected with Ad-SIRT1. (D) Immunoblotting analysis (left) & quantification (right) of insulin-stimulated phosphorylation of IR, AKT & GSK3 beta in hepatocytes. Hepatocytes were treated with 1.2 mM NEFA or 1.2 mM NEFA & Ad-SIRT1 with or without 100 nM insulin. (E, F & G) Hepatocytes were treated with 1.2 mM NEFA or 1.2 mM NEFA & Ad-SIRT1. (E) Glucose concentration in medium. (F) Immunoblot analysis (top) & quantification (bottom) of SREBP-1c & PPAR alpha in hepatocytes. (G) TG contents in hepatocytes. *P < 0.05, **P < 0.01. All experiments were repeated at least three times & representative results are shown. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29207650), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2215_rabbit-polyclonal-srebp1-antibody-310202415541953.jpg)
![Western Blot: STAT5A Antibody (OTI9F7) [NBP2-00622] - Analysis of extracts (35ug) from 9 different cell lines by using anti-STAT5A monoclonal antibody.](http://images.novusbio.com/fullsize/STAT5A-Antibody-OTI9F7-Western-Blot-NBP2-00622-img0011.jpg "Western Blot: STAT5A Antibody (OTI9F7) [NBP2-00622] - Analysis of extracts (35ug) from 9 different cell lines by using anti-STAT5A monoclonal antibody.")
![Immunohistochemistry-Paraffin: STAT5A Antibody (OTI9F7) [NBP2-00622] - Staining of paraffin-embedded Human prostate tissue using anti-STAT5A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/STAT5A-Antibody-OTI9F7-Immunohistochemistry-Paraffin-NBP2-00622-img0010.jpg)
![Flow Cytometry: STAT5A Antibody (OTI9F7) [NBP2-00622] - Analysis of Jurkat cells, using anti-STAT5A antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/STAT5A-Antibody-OTI9F7-Flow-Cytometry-NBP2-00622-img0002.jpg)
![Western Blot: STAT5A Antibody (OTI9F7) [NBP2-00622] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STAT5A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STAT5A.](http://images.novusbio.com/fullsize/STAT5A-Antibody-OTI9F7-Western-Blot-NBP2-00622-img0003.jpg)
![Immunohistochemistry-Paraffin: STAT5A Antibody (OTI9F7) [NBP2-00622] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-STAT5A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/STAT5A-Antibody-OTI9F7-Immunohistochemistry-Paraffin-NBP2-00622-img0004.jpg)
![Immunohistochemistry-Paraffin: STAT5A Antibody (OTI9F7) [NBP2-00622] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-STAT5A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/STAT5A-Antibody-OTI9F7-Immunohistochemistry-Paraffin-NBP2-00622-img0005.jpg)
![Immunohistochemistry-Paraffin: STAT5A Antibody (OTI9F7) [NBP2-00622] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-STAT5A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/STAT5A-Antibody-OTI9F7-Immunohistochemistry-Paraffin-NBP2-00622-img0006.jpg)
![Immunohistochemistry-Paraffin: STAT5A Antibody (OTI9F7) [NBP2-00622] - Staining of paraffin-embedded Human Kidney tissue using anti-STAT5A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/STAT5A-Antibody-OTI9F7-Immunohistochemistry-Paraffin-NBP2-00622-img0007.jpg)
![Immunohistochemistry-Paraffin: STAT5A Antibody (OTI9F7) [NBP2-00622] - Staining of paraffin-embedded Human lymph node tissue using anti-STAT5A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/STAT5A-Antibody-OTI9F7-Immunohistochemistry-Paraffin-NBP2-00622-img0008.jpg)
![Immunohistochemistry-Paraffin: STAT5A Antibody (OTI9F7) [NBP2-00622] - Staining of paraffin-embedded Human lymphoma tissue using anti-STAT5A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/STAT5A-Antibody-OTI9F7-Immunohistochemistry-Paraffin-NBP2-00622-img0009.jpg)
![Flow Cytometry: STAT5A Antibody (OTI9F7) [NBP2-00622] - Analysis of Hela cells, using anti-STAT5A antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/STAT5A-Antibody-OTI9F7-Flow-Cytometry-NBP2-00622-img0001.jpg)
![Western Blot: ER alpha/NR3A1 Antibody (33) [NB300-560] - Analysis of recombinant ER alpha.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-33-Western-Blot-NB300-560-img0004.jpg "Western Blot: ER alpha/NR3A1 Antibody (33) [NB300-560] - Analysis of recombinant ER alpha.")
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (33) [NB300-560] - Human breast tissue (B) and magnified section (C) compared with an isotype control (A).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-33-Immunohistochemistry-Paraffin-NB300-560-img0010.jpg)
![Immunohistochemistry-Frozen: ER alpha/NR3A1 Antibody (33) [NB300-560] - Human breast carcinoma (B) and magnified section (C) compared with an isotype control (A).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-33-Immunohistochemistry-Frozen-NB300-560-img0009.jpg)
![Western Blot: ER alpha/NR3A1 Antibody [NB100-81909] - Western blot analysis of extracts from MCF7 cells using NB100-81909 and the same antibody preincubated with blocking peptide.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Western-Blot-NB100-81909-img0010.jpg "Western Blot: ER alpha/NR3A1 Antibody [NB100-81909] - Western blot analysis of extracts from MCF7 cells using NB100-81909 and the same antibody preincubated with blocking peptide.")
![Immunocytochemistry/Immunofluorescence: ER alpha/NR3A1 Antibody [NB100-81909] - ICC/IF showing estrogen receptor alpha (green) in human cervical stromal cells. Nuclei are stained with DAPI (blue). Antibody at 1:200 in 3% BSA. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-81909-img0009.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NB100-81909] - Analysis of paraffin-embedded human breast carcinoma tissue using NB100-81909 (left) or the same antibody preincubated with blocking peptide(right).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NB100-81909-img0006.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NB100-81909] - Immunofluorescence analysis of rat-lung tissue.](http://images.novusbio.com/fullsize/nb100-81909_rabbit-polyclonal-er-alpha-nr3a1-antibody-23420241523500.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NB100-81909] - Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue.](http://images.novusbio.com/fullsize/nb100-81909_rabbit-polyclonal-er-alpha-nr3a1-antibody-2342024155515.jpg)
![Immunocytochemistry/ Immunofluorescence: ER alpha/NR3A1 Antibody [NB100-81909] - Immunofluorescence analysis of Hela cells.](http://images.novusbio.com/fullsize/nb100-81909_rabbit-polyclonal-er-alpha-nr3a1-antibody-234202414521010.jpg)
![Western Blot: ER alpha/NR3A1 Antibody [NB100-81909] - Western blot analysis of lysates from 293 cells, using NB100-81909. The lane on the right is blocked with the synthesized peptide.](http://images.novusbio.com/fullsize/nb100-81909_rabbit-polyclonal-er-alpha-nr3a1-antibody-234202414462211.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NB100-81909] - Immunohistochemical analysis of paraffin-embedded Human-lung tissue.](http://images.novusbio.com/fullsize/nb100-81909_rabbit-polyclonal-er-alpha-nr3a1-antibody-23420241521260.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84827] - Analysis in human endometrium and cerebral cortex tissues. Corresponding ER alpha/NR3A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84827-img0018.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84827] - Analysis in human endometrium and cerebral cortex tissues. Corresponding ER alpha/NR3A1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84827] - Staining of human cerebral cortex, cervix, uterine, endometrium and gastrointestinal using Anti-ER alpha/NR3A1 antibody NBP1-84827 (A) shows similar protein distribution across tissues to independent antibody NBP1-84826 (B).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84827-img0021.jpg)
![Immunocytochemistry/Immunofluorescence: ER alpha/NR3A1 Antibody [NBP1-84827] - Staining of human cell line MCF7 shows localization to nucleus & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84827-img0006.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84827] - Staining of human uterine cervix shows strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84827-img0024.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84827] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84827-img0019.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84827] - Staining of human endometrium shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84827-img0022.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84827] - Staining of human small intestine shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84827-img0023.jpg)
![Western Blot: STAT5A Antibody [NBP1-81051] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/STAT5A-Antibody-Western-Blot-NBP1-81051-img0008.jpg "Western Blot: STAT5A Antibody [NBP1-81051] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).")
![Immunocytochemistry/Immunofluorescence: STAT5A Antibody [NBP1-81051] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/STAT5A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81051-img0009.jpg)
![Immunohistochemistry-Paraffin: STAT5A Antibody [NBP1-81051] - Staining of human lymph node shows moderate to strong cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/STAT5A-Antibody-Immunohistochemistry-Paraffin-NBP1-81051-img0014.jpg)
![Western Blot: STAT5A Antibody [NBP1-81051] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/STAT5A-Antibody-Western-Blot-NBP1-81051-img0005.jpg)
![Immunohistochemistry-Paraffin: STAT5A Antibody [NBP1-81051] - Staining of human Fallopian tube shows weak cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/STAT5A-Antibody-Immunohistochemistry-Paraffin-NBP1-81051-img0011.jpg)
![Immunohistochemistry-Paraffin: STAT5A Antibody [NBP1-81051] - Staining of human kidney shows moderate to strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/STAT5A-Antibody-Immunohistochemistry-Paraffin-NBP1-81051-img0012.jpg)
![Immunohistochemistry-Paraffin: STAT5A Antibody [NBP1-81051] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/STAT5A-Antibody-Immunohistochemistry-Paraffin-NBP1-81051-img0013.jpg)
![Western Blot: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis using ESR1 mAb against HEK293 (1) and ESR1 (AA: 2-185)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-Western-Blot-NBP2-61764-img0008.jpg "Western Blot: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis using ESR1 mAb against HEK293 (1) and ESR1 (AA: 2-185)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis of Hela cells using ESR1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-Immunocytochemistry-Immunofluorescence-NBP2-61764-img0004.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis of esophageal cancer tissues using ESR1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-Immunohistochemistry-NBP2-61764-img0006.jpg)
![Flow Cytometry: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis of Hela cells using ESR1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-ELISA-NBP2-61764-img0002.jpg)
![Western Blot: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis using ESR1 mAb against human ESR1 (AA: 2-185) recombinant protein. (Expected MW is 45.8 kDa)](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-Western-Blot-NBP2-61764-img0007.jpg)
![Western Blot: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis using ESR1 mouse mAb against MOLT4 (1), Raji (2), MCF-7 (3), T47D (4), SK-Br-3 (5), Hela (6), and C6 (7) cell lysate.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-Western-Blot-NBP2-61764-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis of Hela cells using ESR1 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-Immunocytochemistry-Immunofluorescence-NBP2-61764-img0003.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis of breast cancer tissues using ESR1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-Immunohistochemistry-NBP2-61764-img0005.jpg)
![ELISA: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-ELISA-NBP2-61764-img0001.jpg)
![Western Blot: YB1 Antibody (9J7M4) [NBP3-16213] - Analysis of extracts of various cell lines, using YB1 Rabbit mAb (NBP3-16213) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/YB1-Antibody-9J7M4-Western-Blot-NBP3-16213-img0008.jpg "Western Blot: YB1 Antibody (9J7M4) [NBP3-16213] - Analysis of extracts of various cell lines, using YB1 Rabbit mAb (NBP3-16213) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: YB1 Antibody (9J7M4) [NBP3-16213] - Analysis of extracts of HeLa cells, using YB1 Rabbit mAb (NBP3-16213) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/YB1-Antibody-9J7M4-Western-Blot-NBP3-16213-img0007.jpg)
![Immunoprecipitation: YB1 Antibody (9J7M4) [NBP3-16213] - Immunoprecipitation of YB1 from 200 ug extracts of HeLa cells was performed using 0.5 ug of YB1 Rabbit mAb . Rabbit IgG isotype control was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using YB1 Rabbit mAb at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-16213_rabbit-yb1-mab-9j7m4-5220251255573.jpg)
![Immunocytochemistry/ Immunofluorescence: YB1 Antibody (9J7M4) [NBP3-16213] - Confocal imaging of NIH/3T3 cells using YB1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L).The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16213_rabbit-yb1-mab-9j7m4-52202511361248.jpg)
![Immunohistochemistry: YB1 Antibody (9J7M4) [NBP3-16213] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using YB1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16213_rabbit-yb1-mab-9j7m4-5220251215454.jpg)
![Immunohistochemistry: YB1 Antibody (9J7M4) [NBP3-16213] - Immunohistochemistry analysis of paraffin-embedded Human colon using YB1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16213_rabbit-yb1-mab-9j7m4-52202511374130.jpg)
![Immunohistochemistry: YB1 Antibody (9J7M4) [NBP3-16213] - Immunohistochemistry analysis of paraffin-embedded Rat kidney using YB1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16213_rabbit-yb1-mab-9j7m4-52202511411489.jpg)
![Immunohistochemistry: YB1 Antibody (9J7M4) [NBP3-16213] - Immunohistochemistry analysis of paraffin-embedded Rat colon using YB1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16213_rabbit-yb1-mab-9j7m4-5220251140277.jpg)
![Immunohistochemistry: YB1 Antibody (9J7M4) [NBP3-16213] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using YB1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16213_rabbit-yb1-mab-9j7m4-52202511392221.jpg)
![Immunocytochemistry/ Immunofluorescence: YB1 Antibody (9J7M4) [NBP3-16213] - Confocal imaging of U-2 OS cells using YB1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L).The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16213_rabbit-yb1-mab-9j7m4-52202511374198.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84826] - Analysis in human endometrium and cerebral cortex tissues. Corresponding ER alpha/NR3A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84826-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84826] - Analysis in human endometrium and cerebral cortex tissues. Corresponding ER alpha/NR3A1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84826] - Staining of human cerebral cortex, cervix, uterine, endometrium and gastrointestinal using Anti-ER alpha/NR3A1 antibody NBP1-84826 (A) shows similar protein distribution across tissues to independent antibody NBP1-84827 (B).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84826-img0016.jpg)
![Western Blot: ER alpha/NR3A1 Antibody [NBP1-84826] - Analysis in control (vector only transfected HEK293T lysate) and ESR1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Western-Blot-NBP1-84826-img0007.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84826] - Staining of human uterine cervix shows strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84826-img0019.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84826] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84826-img0014.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84826] - Staining of human endometrium shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84826-img0017.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84826] - Staining of human small intestine shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84826-img0018.jpg)
![Western Blot: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ESR1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-CL1196-Western-Blot-NBP2-34478-img0008.jpg "<b>Genetic Strategies Validation. </b>Western Blot: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ESR1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Staining of MCF7 cells using the Anti-ESR1 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-CL1196-Immunocytochemistry-Immunofluorescence-NBP2-34478-img0009.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Staining of human testis shows absence of immunoreactivity (negative control).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-CL1196-Immunohistochemistry-NBP2-34478-img0005.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Staining of human endometrium shows strong nuclear immunoreactivity in glandular cells.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-CL1196-Immunohistochemistry-NBP2-34478-img0001.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Staining of human cervix shows strong nuclear positivity in epithelial cells.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-CL1196-Immunohistochemistry-NBP2-34478-img0002.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Staining of human fallopian tube shows strong immunoreactivity in epithelial cells.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-CL1196-Immunohistochemistry-NBP2-34478-img0003.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Staining of human breast cancer shows moderate to strong nuclear positivity in a subset of tumor cells.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-CL1196-Immunohistochemistry-NBP2-34478-img0004.jpg)
![Western Blot: Estrogen Receptor alpha Antibody (1B1) [NBP2-01371] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Estrogen Receptor alpha (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Estrogen Receptor alpha.](http://images.novusbio.com/fullsize/Estrogen-Receptor-alpha-Antibody-1B1-Western-Blot-NBP2-01371-img0001.jpg "Western Blot: Estrogen Receptor alpha Antibody (1B1) [NBP2-01371] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Estrogen Receptor alpha (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Estrogen Receptor alpha.")
![Immunohistochemistry-Paraffin: Estrogen Receptor alpha Antibody (1B1) [NBP2-01371] Staining of paraffin-embedded Human tonsil using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Estrogen-Receptor-alpha-Antibody-1B1-Immunohistochemistry-Paraffin-NBP2-01371-img0009.jpg)
![Immunohistochemistry-Paraffin: Estrogen Receptor alpha Antibody (1B1) [NBP2-01371] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Estrogen-Receptor-alpha-Antibody-1B1-Immunohistochemistry-Paraffin-NBP2-01371-img0002.jpg)
![Immunohistochemistry-Paraffin: Estrogen Receptor alpha Antibody (1B1) [NBP2-01371] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Estrogen-Receptor-alpha-Antibody-1B1-Immunohistochemistry-Paraffin-NBP2-01371-img0003.jpg)
![Immunohistochemistry-Paraffin: Estrogen Receptor alpha Antibody (1B1) [NBP2-01371] - Staining of paraffin-embedded Human breast tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Estrogen-Receptor-alpha-Antibody-1B1-Immunohistochemistry-Paraffin-NBP2-01371-img0004.jpg)
![Immunohistochemistry-Paraffin: Estrogen Receptor alpha Antibody (1B1) [NBP2-01371] - Staining of paraffin-embedded Human Kidney tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Estrogen-Receptor-alpha-Antibody-1B1-Immunohistochemistry-Paraffin-NBP2-01371-img0005.jpg)
![Immunohistochemistry-Paraffin: Estrogen Receptor alpha Antibody (1B1) [NBP2-01371] - Staining of paraffin-embedded Human lymphoma tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Estrogen-Receptor-alpha-Antibody-1B1-Immunohistochemistry-Paraffin-NBP2-01371-img0006.jpg)
![Immunohistochemistry-Paraffin: Estrogen Receptor alpha Antibody (1B1) [NBP2-01371] - Staining of paraffin-embedded Human pancreas tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Estrogen-Receptor-alpha-Antibody-1B1-Immunohistochemistry-Paraffin-NBP2-01371-img0007.jpg)
![Immunohistochemistry-Paraffin: Estrogen Receptor alpha Antibody (1B1) [NBP2-01371] - Staining of paraffin-embedded Human prostate tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Estrogen-Receptor-alpha-Antibody-1B1-Immunohistochemistry-Paraffin-NBP2-01371-img0008.jpg)