| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85769-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08660 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88192-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86928-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85741-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47860-0.1mg | |||
| IHC-Prediluted | NBP2-48317 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-45337 | |||
| Azide and BSA Free | NBP2-34716-0.1mg | |||
![Immunohistochemistry-Paraffin: NGFR/TNFRSF16/p75NTR Antibody [NBP1-85769] - Staining in human spleen and skeletal muscle tissues using NBP1-85769 antibody. Corresponding NGFR RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NGFR-TNFRSF16-p75NTR-Antibody-Immunohistochemistry-Paraffin-NBP1-85769-img0019.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NGFR/TNFRSF16/p75NTR Antibody [NBP1-85769] - Staining in human spleen and skeletal muscle tissues using NBP1-85769 antibody. Corresponding NGFR RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: NGFR/TNFRSF16/p75NTR Antibody [NBP1-85769] - Analysis in human cell line RH-30.](http://images.novusbio.com/fullsize/NGFR-TNFRSF16-p75NTR-Antibody-Western-Blot-NBP1-85769-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: NGFR/TNFRSF16/p75NTR Antibody [NBP1-85769] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NGFR-TNFRSF16-p75NTR-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85769-img0007.jpg)
![Immunohistochemistry-Paraffin: NGFR/TNFRSF16/p75NTR Antibody [NBP1-85769] - Staining of human lymph node shows strong membranous positivity in germinal center cells.](http://images.novusbio.com/fullsize/NGFR-TNFRSF16-p75NTR-Antibody-Immunohistochemistry-Paraffin-NBP1-85769-img0014.jpg)
![Immunohistochemistry-Paraffin: NGFR/TNFRSF16/p75NTR Antibody [NBP1-85769] - Staining of human prostate shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/NGFR-TNFRSF16-p75NTR-Antibody-Immunohistochemistry-Paraffin-NBP1-85769-img0015.jpg)
![Immunohistochemistry-Paraffin: NGFR/TNFRSF16/p75NTR Antibody [NBP1-85769] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/NGFR-TNFRSF16-p75NTR-Antibody-Immunohistochemistry-Paraffin-NBP1-85769-img0016.jpg)
![Immunohistochemistry-Paraffin: NGFR/TNFRSF16/p75NTR Antibody [NBP1-85769] - Staining of human spleen shows strong membranous positivity.](http://images.novusbio.com/fullsize/NGFR-TNFRSF16-p75NTR-Antibody-Immunohistochemistry-Paraffin-NBP1-85769-img0017.jpg)
![Immunohistochemistry-Paraffin: NGFR/TNFRSF16/p75NTR Antibody [NBP1-85769] - Staining of human testis shows strong membranous positivity in peritubular myoid cells.](http://images.novusbio.com/fullsize/NGFR-TNFRSF16-p75NTR-Antibody-Immunohistochemistry-Paraffin-NBP1-85769-img0018.jpg)
![Immunohistochemistry-Paraffin: p63/TP73L Antibody (rTP40/3690) [NBP3-07430] - Formalin-fixed, paraffin-embedded human kidney stained with p63/TP73L Recombinant Mouse Monoclonal Antibody (rTP40/3690).](http://images.novusbio.com/fullsize/p63-TP73L-Antibody-rTP40-3690-Immunohistochemistry-Paraffin-NBP3-07430-img0003.jpg "Immunohistochemistry-Paraffin: p63/TP73L Antibody (rTP40/3690) [NBP3-07430] - Formalin-fixed, paraffin-embedded human kidney stained with p63/TP73L Recombinant Mouse Monoclonal Antibody (rTP40/3690).")
![Flow Cytometry: p63/TP73L Antibody (rTP40/3690) [NBP3-07430] - Flow Cytometric Analysis of PFA-fixed HeLa cells using p63/TP73L Recombinant Mouse Monoclonal Antibody (rTP40/3690) followed by goat anti- Mouse IgG-CF488 (blue); isotype control (red).](http://images.novusbio.com/fullsize/p63-TP73L-Antibody-rTP40-3690-Flow-Cytometry-NBP3-07430-img0001.jpg)
![Immunohistochemistry-Paraffin: p63/TP73L Antibody (rTP40/3690) [NBP3-07430] - Formalin-fixed, paraffin-embedded human skin stained with p63/TP73L Recombinant Mouse Monoclonal Antibody (rTP40/3690).](http://images.novusbio.com/fullsize/p63-TP73L-Antibody-rTP40-3690-Immunohistochemistry-Paraffin-NBP3-07430-img0002.jpg)
![Immunohistochemistry-Paraffin: CXADR Antibody [NBP1-88192] - Analysis in human skin and skeletal muscle tissues using NBP1-88192 antibody. Corresponding CXADR RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CXADR-Antibody-Immunohistochemistry-Paraffin-NBP1-88192-img0018.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CXADR Antibody [NBP1-88192] - Analysis in human skin and skeletal muscle tissues using NBP1-88192 antibody. Corresponding CXADR RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: CXADR Antibody [NBP1-88192] - Analysis in human cell lines Caco-2 and MCF-7. Corresponding CXADR RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/CXADR-Antibody-Western-Blot-NBP1-88192-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: CXADR Antibody [NBP1-88192] - Staining of human cell line U-2 OS shows localization to nucleoplasm, plasma membrane & cell junctions. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CXADR-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88192-img0010.jpg)
![Immunohistochemistry-Paraffin: CXADR Antibody [NBP1-88192] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/CXADR-Antibody-Immunohistochemistry-Paraffin-NBP1-88192-img0012.jpg)
![Immunohistochemistry-Paraffin: CXADR Antibody [NBP1-88192] - Staining of human prostate shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/CXADR-Antibody-Immunohistochemistry-Paraffin-NBP1-88192-img0015.jpg)
![Immunohistochemistry-Paraffin: CXADR Antibody [NBP1-88192] - Staining of human skin shows strong membranous positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/CXADR-Antibody-Immunohistochemistry-Paraffin-NBP1-88192-img0016.jpg)
![Immunohistochemistry-Paraffin: CXADR Antibody [NBP1-88192] - Staining of human testis shows strong membranous positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/CXADR-Antibody-Immunohistochemistry-Paraffin-NBP1-88192-img0017.jpg)
![Western Blot: TOP2A Antibody [NBP1-86928] - MDA-MB-231 and MCF-7 whole cell lysates were loaded (40 ug/lane). 10% SDS-PAGE. TOP2A Antibody (NBP1-86928) primary antibody at 1:1000, 4C, overnight. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/TOP2A-Antibody-Western-Blot-NBP1-86928-img0016.jpg "Western Blot: TOP2A Antibody [NBP1-86928] - MDA-MB-231 and MCF-7 whole cell lysates were loaded (40 ug/lane). 10% SDS-PAGE. TOP2A Antibody (NBP1-86928) primary antibody at 1:1000, 4C, overnight. WB image submitted by a verified customer review.")
![Immunohistochemistry-Paraffin: TOP2A Antibody [NBP1-86928] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/TOP2A-Antibody-Immunohistochemistry-Paraffin-NBP1-86928-img0013.jpg)
![Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody [NBP1-85741] - Analysis in human placenta and pancreas tissues. Corresponding TfR (Transferrin R) RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-Immunohistochemistry-Paraffin-NBP1-85741-img0015.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody [NBP1-85741] - Analysis in human placenta and pancreas tissues. Corresponding TfR (Transferrin R) RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: TfR (Transferrin R) Antibody [NBP1-85741] - Staining of human cell line U-251 MG shows localization to endosomes and lysosomes. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85741-img0014.jpg)
![Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody [NBP1-85741] - Staining of human placenta shows strong membranous positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-Immunohistochemistry-Paraffin-NBP1-85741-img0019.jpg)
![Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody [NBP1-85741] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-Immunohistochemistry-Paraffin-NBP1-85741-img0016.jpg)
![Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody [NBP1-85741] - Staining of human lung shows strong cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-Immunohistochemistry-Paraffin-NBP1-85741-img0017.jpg)
![Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody [NBP1-85741] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-Immunohistochemistry-Paraffin-NBP1-85741-img0018.jpg)
![Western Blot: TRAP1 Antibody (TRAP1-6) [NB300-555] - Figure 2 illustrates IP of TRAP1 visualized by Coomassie Blue staining.](http://images.novusbio.com/fullsize/TRAP1-Antibody-TRAP1-6-Western-Blot-NB300-555-img0005.jpg "Western Blot: TRAP1 Antibody (TRAP1-6) [NB300-555] - Figure 2 illustrates IP of TRAP1 visualized by Coomassie Blue staining.")
![Immunocytochemistry/Immunofluorescence: TRAP1 Antibody (TRAP1-6) [NB300-555] - Analysis of TRAP1 in NCI-H460 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a TRAP1 monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. TRAP1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/TRAP1-Antibody-TRAP1-6-Immunocytochemistry-Immunofluorescence-NB300-555-img0006.jpg)
![Immunohistochemistry-Paraffin: TRAP1 Antibody (TRAP1-6) [NB300-555] - Normal biopsies of deparaffinized Human liver tissue, dilution of 1:20.](http://images.novusbio.com/fullsize/TRAP1-Antibody-TRAP1-6-Immunohistochemistry-Paraffin-NB300-555-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: TRAP1 Antibody (TRAP1-6) [NB300-555] - Immunolocalization of TRAP1 in PC-3-M cells.](http://images.novusbio.com/fullsize/TRAP1-Antibody-TRAP1-6-Immunocytochemistry-Immunofluorescence-NB300-555-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: TRAP1 Antibody (TRAP1-6) [NB300-555] - PGE2 regulates migration & osteoclast differentiation through the G alpha s/PI3K/AKT/MAPK signaling pathways downstream of EP4. PGE2 regulates migration & osteoclast differentiation independent of beta -arrestin1 (a) & beta -arrestin2 (b) but via G alpha s (c). BMMs from the WT & littermate beta -arrestin1- & beta -arrestin2-knockout mice were used to generate osteoclasts by stimulation with 10 ng·mL−1 M-CSF & 50 ng·mL−1 RANKL & incubation with 100 nmol·L−1 PGE2. For G alpha s experiments, osteoclasts were generated from the GMMs of the G alpha sfl/fl controls & G alpha sfl/fl; LysM-cre (G alpha sLysM) mice. A differentiation assay (TRAP staining) (left) & the corresponding quantitative analysis (right). Scale bars, 50 μm. Error bars are the mean ± s.d. n = 3. Two-way ANOVA followed by Tukey’s t tests. Scale bars, 50 μm. d cAMP production was measured via ELISAs for osteoclasts derived from the Ep4fl/fl & Ep4LysM mice. BMMs from the two mouse strains were isolated & stimulated with 10 ng·mL−1 M-CSF & 50 ng·mL−1 RANKL to differentiate into osteoclasts & incubated with 100 nmol·L−1 PGE2 for 30 min prior to cAMP measurements. Error bars are the mean ± s.d. ***P < 0.001 by unpaired two-tailed Student’s t test. The experiment was performed with three biological replicates. e Representative images of the indicated protein expression by western blotting for osteoclasts generated using BMMs of Ep4fl/fl & Ep4LysM mice. The cells were treated either with osteoclastogenic media (10 ng·mL−1 M-CSF & 50 ng·mL−1 RANKL) alone, with PGE2 (100 nmol·L−1), or PGE2 with IBMX (1 mmol·L−1) for 3 h. The experiments were performed with three biological replicates Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35260562), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-555_mouse-monoclonal-trap1-antibody-trap1-6-310202416212330.jpg)
![Western Blot: Tau [p Ser 202, p Thr 205] Antibody (AH36) [NBP3-18265] - Western Blot analysis of Human iPSC-derived cortical neurons showing detection of Tau protein using Rabbit Anti-Tau Monoclonal Antibody, Clone AH36 (NBP3-18265). Lane 1: MW ladder. Lane 2: Control (non-disease) line. Lane 2: Ex10+16 tau mutant sample. Lane 3: P301L tau mutant sample. Load: 50ug. Primary Antibody: Rabbit Anti-Tau Monoclonal Antibody (NBP3-18265) at 1:500 for Overnight. pSer202/pThr205 was detected using NBP3-18265. Total tau was detected using mouse anti-tau antibody (clone HT7). The bar graph on the right shows quantification of pSer202/pThr205 compared to total tau in each sample.](http://images.novusbio.com/fullsize/Tau-p-Ser-202-p-Thr-205-Antibody-AH36-Western-Blot-NBP3-18265-img0002.jpg "Western Blot: Tau [p Ser 202, p Thr 205] Antibody (AH36) [NBP3-18265] - Western Blot analysis of Human iPSC-derived cortical neurons showing detection of Tau protein using Rabbit Anti-Tau Monoclonal Antibody, Clone AH36 (NBP3-18265). Lane 1: MW ladder. Lane 2: Control (non-disease) line. Lane 2: Ex10+16 tau mutant sample. Lane 3: P301L tau mutant sample. Load: 50ug. Primary Antibody: Rabbit Anti-Tau Monoclonal Antibody (NBP3-18265) at 1:500 for Overnight. pSer202/pThr205 was detected using NBP3-18265. Total tau was detected using mouse anti-tau antibody (clone HT7). The bar graph on the right shows quantification of pSer202/pThr205 compared to total tau in each sample.")
![Immunocytochemistry/Immunofluorescence: Tau [p Ser 202, p Thr 205] Antibody (AH36) [NBP3-18265] - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Tau Monoclonal Antibody, Clone AH36 (NBP3-18265). Tissue: iPSC-derived cortical excitatory neurons. Species: Human. Primary Antibody: Rabbit Anti-Tau Monoclonal Antibody (NBP3-18265) at 1:500 for Overnight. Secondary Antibody: Donkey anti-rabbit: Alexa Fluor 488 at 1:1000. Counterstain: DAPI. A) iPSC-derived neurons from non-demented control (NDC). B) iPSC-derived neurons from subject with P301L MAPT mutation. Images acquired using an automated Opera Phoenix system. Each field of view is a max projection from 10 planes of 1 um stacks.](http://images.novusbio.com/fullsize/Tau-p-Ser-202-p-Thr-205-Antibody-AH36-Immunocytochemistry-Immunofluorescence-NBP3-18265-img0001.jpg)
![Dot Blot: Tau [p Ser 202, p Thr 205] Antibody (AH36) [NBP3-18265] - Dot Blot analysis using Rabbit Anti-Tau Monoclonal Antibody, Clone AH36 (NBP3-18265). Species: E. Coli, Baculovirus. Primary Antibody: Rabbit Anti-Tau Monoclonal Antibody (NBP3-18265) at 1:500. Secondary Antibody: Goat anti-rabbit IgG:HRP.](http://images.novusbio.com/fullsize/Tau-p-Ser-202-p-Thr-205-Antibody-AH36-Dot-Blot-NBP3-18265-img0003.jpg)
![Western Blot: Nucleolin Antibody (NCL/902) [NBP2-44610] - A431 Cell Lysate using Nucleolin Monoclonal Antibody (NCL/902)](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-902-Western-Blot-NBP2-44610-img0009.jpg "Western Blot: Nucleolin Antibody (NCL/902) [NBP2-44610] - A431 Cell Lysate using Nucleolin Monoclonal Antibody (NCL/902)")
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (NCL/902) [NBP2-44610] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells stained with Nucleolin Antibody (NCL/902) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-902-Immunocytochemistry-Immunofluorescence-NBP2-44610-img0016.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/902) [NBP2-44610] - Formalin-fixed, paraffin-embedded human colon stained with Nucleolin Antibody (NCL/902).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-902-Immunohistochemistry-Paraffin-NBP2-44610-img0021.jpg)
![Flow Cytometry: Nucleolin Antibody (NCL/902) [NBP2-44610] - Flow Cytometry of Human Nucleolin Ag on Jurkat cells. Grey: Isotype Control; Green: FITC-labeled; Purple: APC-Labeled; Turquoise: AF700 Labeled Nucleolin Antibody (NCL/902).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-902-Flow-Cytometry-NBP2-44610-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (NCL/902) [NBP2-44610] - HeLa cells stained with AF488 labeled Nucleolin Antibody (NCL/902) Green: AF488-labeled Ab. Blue: DAPI.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-902-Immunocytochemistry-Immunofluorescence-NBP2-44610-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (NCL/902) [NBP2-44610] - Immunofluorescence Analysis of HeLa cells stained with CF647R labeled Nucleolin Antibody (NCL/902)(Green) Blue: DAPI.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-902-Immunocytochemistry-Immunofluorescence-NBP2-44610-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (NCL/902) [NBP2-44610] - Immunofluorescence Analysis of PFA-fixed K562 cells stained with Nucleolin Antibody (NCL/902) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-902-Immunocytochemistry-Immunofluorescence-NBP2-44610-img0015.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/902) [NBP2-44610] - Human Bladder carcinoma stained with Nucleolin Monoclonal Antibody (NCL/902).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-902-Immunohistochemistry-Paraffin-NBP2-44610-img0003.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/902) [NBP2-44610] - Human Ovarian Carcinoma stained with Nucleolin Monoclonal Antibody (NCL/902).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-902-Immunohistochemistry-Paraffin-NBP2-44610-img0004.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/902) [NBP2-44610] - Human Skin stained with Nucleolin Monoclonal Antibody (NCL/902).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-902-Immunohistochemistry-Paraffin-NBP2-44610-img0005.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/902) [NBP2-44610] - Human Uterus stained with Nucleolin Monoclonal Antibody (NCL/902).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-902-Immunohistochemistry-Paraffin-NBP2-44610-img0008.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/902) [NBP2-44610] - Formalin-fixed, paraffin-embedded human Skin stained with Nucleolin Antibody (NCL/902).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-902-Immunohistochemistry-Paraffin-NBP2-44610-img0018.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/902) [NBP2-44610] - Formalin-fixed, paraffin-embedded human Uterus stained with Nucleolin Antibody (NCL/902).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-902-Immunohistochemistry-Paraffin-NBP2-44610-img0019.jpg)
![Western Blot: SUMO1 Antibody (SM1/495) [NBP2-32901] - Analysis using the Azide and BSA Free version of NBP2-32901. Detection of SUMO1 in human HeLa Cell lysate.](http://images.novusbio.com/fullsize/SUMO1-Antibody-SM1-495-Western-Blot-NBP2-32901-img0004.jpg "Western Blot: SUMO1 Antibody (SM1/495) [NBP2-32901] - Analysis using the Azide and BSA Free version of NBP2-32901. Detection of SUMO1 in human HeLa Cell lysate.")
![Immunocytochemistry/Immunofluorescence: SUMO1 Antibody (SM1/495) [NBP2-32901] - Paraformaldehyde-fixed HepG2 cells stained with SUMO1 Antibody (SM1/495)followed by goat anti-Mouse IgG-CF488 (Green). Cell membranes are stained with Phalloidin (red).](http://images.novusbio.com/fullsize/SUMO1-Antibody-SM1-495-Immunocytochemistry-Immunofluorescence-NBP2-32901-img0007.jpg)
![Immunohistochemistry-Paraffin: SUMO1 Antibody (SM1/495) [NBP2-32901] - Formalin-fixed, paraffin-embedded human tonsil stained with SUMO-1 MAb (SM1/495)](http://images.novusbio.com/fullsize/SUMO1-Antibody-SM1-495-Immunohistochemistry-Paraffin-NBP2-32901-img0001.jpg)
![Flow Cytometry: SUMO1 Antibody (SM1/495) [NBP2-32901] - Flow Cytometric Analysis of paraformaldehyde-fixed HepG2 cells using SUMO1 Antibody (SM1/495) followed by goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/SUMO1-Antibody-SM1-495-Flow-Cytometry-NBP2-32901-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: HNF-3 alpha/FoxA1 Antibody [NBP1-30877] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/HNF-3-alpha-FoxA1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30877-img0008.jpg "Immunocytochemistry/Immunofluorescence: HNF-3 alpha/FoxA1 Antibody [NBP1-30877] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:1000 dilution.")
![Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody [NBP1-30877] - Human cervical carcinoma. FOXA1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/HNF-3-alpha-FoxA1-Antibody-Immunohistochemistry-Paraffin-NBP1-30877-img0007.jpg)
![Chromatin Immunoprecipitation: HNF-3 alpha/FoxA1 Antibody [NBP1-30877] - Cross-linked ChIP was performed with MCF-7 chromatin extract and 5 ug of either control rabbit IgG or anti-FOXA1 antibody. The precipitated DNA was detected by PCR with primer set targeting to TFF1 or HK1.](http://images.novusbio.com/fullsize/HNF-3-alpha-FoxA1-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP1-30877-img0012.jpg)
![Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody [NBP1-30877] - Human breast carcinoma. FOXA1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/HNF-3-alpha-FoxA1-Antibody-Immunohistochemistry-Paraffin-NBP1-30877-img0006.jpg)
![Immunoprecipitation: HNF-3 alpha/FoxA1 Antibody [NBP1-30877] - FOXA1 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 40 ug whole cell lysate/extract of FOXA1 protein expressing HepG2 cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of FOXA1 by 2.5 ug of FOXA1 antibody 10% SDS-PAGE The immunoprecipitated FOXA1 protein was detected by FOXA1 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/HNF-3-alpha-FoxA1-Antibody-Immunoprecipitation-NBP1-30877-img0014.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: HNF-3 alpha/FoxA1 Antibody [NBP1-30877] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with FOXA1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp1-30877_rabbit-polyclonal-hnf-3-alpha-foxa1-antibody-1610202419405736.jpg)
![Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase, Tissue Non-Specific Antibody (TRA-2-49) - BSA Free [NB600-540] - Confocal fluorescent analysis of A431 using anti-TRA-2-49 Mouse monoclonal antibody (Red). Actin filaments have been labeled with AlexaFluor(R) 488-Phalloidin (Green). Nucleus is stained with DAPI (Blue). Positive cytosol staining.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-TRA-2-49-BSA-Free-Immunocytochemistry-Immunofluorescence-NB600-540-img0011.jpg)
![Flow Cytometry: Alkaline Phosphatase, Tissue Non-Specific Antibody (TRA-2-49) [NB600-540] - Analysis using anti-TRA-2-49. 2102Ep cells were stained with anti-TRA-2-49 antibody (Green) or isotype control antibody (grey).](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-TRA-2-49-Flow-Cytometry-NB600-540-img0008.jpg)
![Western Blot: BMI-1 Antibody [NB100-87026] - Detection of human BMI1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, LNCaP, RKO, Hep-G2, U2OS, A-549, Jurkat, K-562, MCF-7, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-BMI1 antibody NB100-87026 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-RPS3.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NB100-87026-img0016.jpg "Western Blot: BMI-1 Antibody [NB100-87026] - Detection of human BMI1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, LNCaP, RKO, Hep-G2, U2OS, A-549, Jurkat, K-562, MCF-7, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-BMI1 antibody NB100-87026 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-RPS3.")
![Immunohistochemistry-Paraffin: BMI-1 Antibody [NB100-87026] - Human pancreatic islet cell carcinoma (left) and mouse renal cell carcinoma (right). Antibody used at a dilution of 1:1000 (1ug/ml).](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunohistochemistry-Paraffin-NB100-87026-img0010.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: BMI-1 Antibody [NB100-87026] - Detection of Human and Mouse BMI1 on HeLa whole cell lysate using NB100-87026. BMI1 was also immunoprecipitated by another rabbit anti-BMI1 antibody.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NB100-87026-img0011.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: BMI-1 Antibody [NB100-87026] - Detection of Human and Mouse BMI1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-BMI1 antibody NB100-87026 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NB100-87026-img0013.jpg)
![Western Blot: BMI-1 Antibody [NB100-87026] - Detection of mouse BMI1 by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-BMI1 antibody NB100-87026 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-RPS3.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NB100-87026-img0014.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: BMI-1 Antibody [NB100-87026] - Detection of human BMI1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BMI1 antibody NB100-87026 used for IP at 6 ug/mg lysate. BMI1 was also immunoprecipitated by another rabbit anti-BMI1 antibody. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunoprecipitation-NB100-87026-img0015.jpg)