Stem-Cell-Markers reagents: Novus Biologicals

NBP2-46630

MBP Antibody (CL2819) - BSA Free

(1 Publication)

0.1 ml

NBP2-46630

Immunohistochemistry: MBP Antibody (CL2819) [NBP2-46630] - Staining of human tonsil shows absence of immunoreactivity (negative control).

Western Blot: MBP Antibody (CL2819) [NBP2-46630] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue

Immunohistochemistry: MBP Antibody (CL2819) [NBP2-46630] - Staining of rat cerebral cortex shows strong immunoreactivity in myelinated neural fibers.

Immunohistochemistry: MBP Antibody (CL2819) [NBP2-46630] - Staining of mouse cerebral cortex shows strong positivity in myelinated neural fibers.

Immunohistochemistry: MBP Antibody (CL2819) [NBP2-46630] - Staining of human cerebral cortex shows strong immunoreactivity in myelinated fibers.

Immunohistochemistry: MBP Antibody (CL2819) [NBP2-46630] - Staining of mouse striatum shows strong positivity in myelinated fibers.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-43790AF350
Alexa Fluor 405	NBP3-43790AF405
Alexa Fluor 488	NBP3-43790AF488
Alexa Fluor 532	NBP3-43790AF532
Alexa Fluor 594	NBP3-43790AF594
Alexa Fluor 647	NBP3-43790AF647
Alexa Fluor 700	NBP3-43790AF700
Alexa Fluor 750	NBP3-43790AF750
Allophycocyanin	NBP3-43790APC
Biotin	NBP3-43790B
CoraFluor 1	NBP3-43790CL1
DyLight 350	NBP3-43790UV
DyLight 405	NBP3-43790V
DyLight 488	NBP3-43790G
DyLight 550	NBP3-43790R
DyLight 594	NBP3-43790DL594
DyLight 650	NBP3-43790C
DyLight 680	NBP3-43790FR
DyLight 755	NBP3-43790IR
FITC	NBP3-43790F
HRP	NBP3-43790H
Janelia Fluor 525	NBP3-43790JF525
Janelia Fluor 549	NBP3-43790JF549
Janelia Fluor 646	NBP3-43790JF646
Janelia Fluor 669	NBP3-43790JF669
PE	NBP3-43790PE
PerCP	NBP3-43790PCP
mFluor Violet 450 SE	NBP3-43790MFV450
mFluor Violet 500 SE	NBP3-43790MFV500
mFluor Violet 610 SE	NBP3-43790MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-43790
BSA Free	NBP2-46630-25ul

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NBP2-01245

CDKN3 Antibody (OTI2E11)

(1 Publication)

0.1 ml

NBP2-01245

Immunohistochemistry-Paraffin: CDKN3 Antibody (2E11) [NBP2-01245] - Staining of paraffin-embedded Human prostate tissue using anti-CDKN3 mouse monoclonal antibody.

Flow Cytometry: CDKN3 Antibody (2E11) [NBP2-01245] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CDKN3 antibody, and then analyzed by flow cytometry.

Immunohistochemistry-Paraffin: CDKN3 Antibody (2E11) [NBP2-01245] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CDKN3 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: CDKN3 Antibody (2E11) [NBP2-01245] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CDKN3 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: CDKN3 Antibody (2E11) [NBP2-01245] - Staining of paraffin-embedded Human endometrium tissue using anti-CDKN3 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: CDKN3 Antibody (2E11) [NBP2-01245] - Staining of paraffin-embedded Human Kidney tissue using anti-CDKN3 mouse monoclonal antibody.

Flow Cytometry: CDKN3 Antibody (2E11) [NBP2-01245] - Analysis of Jurkat cells, using anti-CDKN3 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-70399AF350
Alexa Fluor 405	NBP2-70399AF405
Alexa Fluor 488	NBP2-70399AF488
Alexa Fluor 532	NBP2-70399AF532
Alexa Fluor 594	NBP2-70399AF594
Alexa Fluor 647	NBP2-70399AF647
Alexa Fluor 700	NBP2-70399AF700
Alexa Fluor 750	NBP2-70399AF750
Allophycocyanin	NBP2-70399APC
Allophycocyanin/Cy7	NBP2-70399APCCY7
Biotin	NBP2-70399B
CoraFluor 1	NBP2-70399CL1
DyLight 350	NBP2-70399UV
DyLight 405	NBP2-70399V
DyLight 488	NBP2-70399G
DyLight 550	NBP2-70399R
DyLight 594	NBP2-70399DL594
DyLight 650	NBP2-70399C
DyLight 680	NBP2-70399FR
DyLight 755	NBP2-70399IR
FITC	NBP2-70399F
HRP	NBP2-70399H
Janelia Fluor 525	NBP2-70399JF525
Janelia Fluor 549	NBP2-70399JF549
Janelia Fluor 646	NBP2-70399JF646
Janelia Fluor 669	NBP2-70399JF669
PE	NBP2-70399PE
PE/Atto594	NBP2-70399PEATT594
PE/Cy5.5	NBP2-70399PECY55
PE/Cy7	NBP2-70399PECY7
PerCP	NBP2-70399PCP
mFluor Violet 450 SE	NBP2-70399MFV450
mFluor Violet 500 SE	NBP2-70399MFV500
mFluor Violet 610 SE	NBP2-70399MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-70399

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NBP2-22481

LIN-28A Antibody (14E6-4E6)

100 μg

NBP2-22481

Immunohistochemistry-Paraffin: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis showing staining in the cytoplasm of human seminoma (right) compared with a negative control without primary antibody (left).

Flow Cytometry: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis of Lin28 (blue histogram) on H9 embryonic stem cells. To generate single cells suspensions, colonies were treated with TrypLE cell dissociation enzyme for 5 minutes at 37C. Cells were incubated with a Lin28 monoclonal antibody or mouse IgG (green histogram) at a dilution of 1:100 for 1 hour on ice, washed with PBS + 5% fetal calf serum (FACS buffer), and incubated with a fluorescein-conjugated secondary antibody at a dilution of 1:200 for 30 minutes on ice. Cells were washed with cold FACS buffer, resuspended in 500ul of FACS buffer containing 10ul of 4% paraformaldehyde.

Chromatin Immunoprecipitation: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis performed using cross-linked chromatin from rat hepatoma cells treated with insulin. IP performed using a multiplex microplate Matrix ChIP assay of LIN28 monoclonal antibody. Chromatin aliquots from cells were used per ChIP pull-down. Quantitative PCR data done in quadruplicate using 1ul of DNA in 2ul SYBR real-time PCR reactions containing primers to amplify -15kb upstream of Egr1 or exon-1 or exon-2-3 of Egr1. Quantitation of immunoprecipitated chromatin is presented as signal relative to the total amount of input chromatin. Results represent the mean +/- SEM. A schematic representation of the rat Egr-1 locus is shown; oxes represent exons (black boxes = translated, white boxes = untranslated), the zigzag line represents an intron, and the straight line represents upstream sequence. Regions amplified by Egr-1 primers are represented by black bars. Data courtesy of the Innovators Program.

Immunocytochemistry/Immunofluorescence: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis of Lin28 (green) in H9 embryonic stem cells grown for a few days on Matrigel-coated chamber slides. Cells fixed in 4% paraformaldehyde were permeabilized with 0.1% Triton X-100 for 15 minutes at room temperature. Cells were probed with a Lin28 monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBST, and incubated with a fluorescein-conjugated secondary antibody at a dilution of 1:100 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI and cells were analyzed by fluorescence microscopy at 20X magnification.

Immunocytochemistry/Immunofluorescence: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis of Lin28 (green) in HEL 11.4 induced IPS cells grown for a few days on Matrigel-coated chamber slides. Cells fixed in 4% paraformaldehyde were permeabilized with 0.1% Triton X-100 for 15 minutes at room temperature. Cells were probed with a Lin28 monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBST, and incubated with a fluorescein-conjugated secondary antibody at a dilution of 1:100 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI and cells were analyzed by fluorescence microscopy at 20X magnification.

Immunohistochemistry-Paraffin: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis showing staining in the nucleus and cytoplasm of mouse testis tissue (right) compared with a negative control without primary antibody (left).

Flow Cytometry: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis of Lin28 (blue histogram) on HEL 11.4 induced IPS cells. To generate single cells suspensions, colonies were treated with TrypLE cell dissociation enzyme for 5 minutes at 37C. Cells were incubated with a Lin28 monoclonal antibody or mouse IgG (green histogram) at a dilution of 1:100 for 1 hour on ice, washed with PBS + 5% fetal calf serum (FACS buffer), and incubated with a fluorescein-conjugated secondary antibody at a dilution of 1:200 for 30 minutes on ice. Cells were washed with cold FACS buffer, resuspended in 500ul of FACS buffer containing 10ul of 4% paraformaldehyde.

Immunoprecipitation: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis of LIN28 was performed. Antigen-antibody complexes were formed by incubating 700ug of lysate with 5 ug of an LIN28 monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50 ul Protein A/G Agarose was loaded as a positive control for detection. Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a LIN28 monoclonal antibody at a dilution of 1:1000 overnight rotating at 4C, washed in TBST, and probed with Clean-blot IP Detection Reagent at a dilution of 1:1000 for at least 1 hour.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP1-47754

Fumarase Antibody (OTI1F10)

(1 Publication)

0.1 ml

NBP1-47754

Immunohistochemistry: Fumarase Antibody (OTI1F10) [NBP1-47754] - Fumarase Antibody (1F10) [NBP1-47754] - Staining of paraffin-embedded Human colon tissue using anti-Fumarase mouse monoclonal antibody.

Flow Cytometry: Fumarase Antibody (1F10) [NBP1-47754] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Fumarase antibody, and then analyzed by flow cytometry.

Western Blot: Fumarase Antibody (1F10) [NBP1-47754] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Fumarase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Fumarase.

Immunohistochemistry-Paraffin: Fumarase Antibody (1F10) [NBP1-47754] - Staining of paraffin-embedded Human endometrium tissue using anti-Fumarase mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Fumarase Antibody (1F10) [NBP1-47754] - Staining of paraffin-embedded Human Kidney tissue using anti-Fumarase mouse monoclonal antibody.

Reactivity:

Hu, Mu, Rt, Ca

Species Glossary

Reactivity: Hu, Mu, Rt, Ca

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-70733AF350
Alexa Fluor 405	NBP2-70733AF405
Alexa Fluor 488	NBP2-70733AF488
Alexa Fluor 532	NBP2-70733AF532
Alexa Fluor 594	NBP2-70733AF594
Alexa Fluor 647	NBP2-70733AF647
Alexa Fluor 700	NBP2-70733AF700
Alexa Fluor 750	NBP2-70733AF750
Allophycocyanin	NBP2-70733APC
Biotin	NBP2-70733B
CoraFluor 1	NBP2-70733CL1
DyLight 350	NBP2-70733UV
DyLight 405	NBP2-70733V
DyLight 488	NBP2-70733G
DyLight 550	NBP2-70733R
DyLight 594	NBP2-70733DL594
DyLight 650	NBP2-70733C
DyLight 680	NBP2-70733FR
DyLight 755	NBP2-70733IR
FITC	NBP2-70733F
HRP	NBP2-70733H
Janelia Fluor 525	NBP2-70733JF525
Janelia Fluor 549	NBP2-70733JF549
Janelia Fluor 646	NBP2-70733JF646
Janelia Fluor 669	NBP2-70733JF669
PE	NBP2-70733PE
PerCP	NBP2-70733PCP
mFluor Violet 450 SE	NBP2-70733MFV450
mFluor Violet 500 SE	NBP2-70733MFV500
mFluor Violet 610 SE	NBP2-70733MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-70733

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NBP1-48002

SSB Antibody (OTI2C8)

0.1 ml

NBP1-48002

Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Human prostate tissue using anti-SSB mouse monoclonal antibody.

Western Blot: SSB Antibody (OTI2C8) [NBP1-48002] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSB.

Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SSB mouse monoclonal antibody.

Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SSB mouse monoclonal antibody.

Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SSB mouse monoclonal antibody.

Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Human breast tissue using anti-SSB mouse monoclonal antibody.

Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Human colon tissue using anti-SSB mouse monoclonal antibody.

Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Human endometrium tissue using anti-SSB mouse monoclonal antibody.

Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Human Kidney tissue using anti-SSB mouse monoclonal antibody.

Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Human lung tissue using anti-SSB mouse monoclonal antibody.

Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Human Ovary tissue using anti-SSB mouse monoclonal antibody.

Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Human pancreas tissue using anti-SSB mouse monoclonal antibody.

Immunoprecipitation: SSB Antibody (OTI2C8) [NBP1-48002] - (Negative control: IP without adding anti-SSB antibody.). For each experiment, 500ul of DDK tagged SSB overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-SSB antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Reactivity:

Hu, Ca, Pm, Pm

Species Glossary

Reactivity: Hu, Ca, Pm, Pm

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-74356AF350
Alexa Fluor 405	NBP2-74356AF405
Alexa Fluor 488	NBP2-74356AF488
Alexa Fluor 532	NBP2-74356AF532
Alexa Fluor 594	NBP2-74356AF594
Alexa Fluor 647	NBP2-74356AF647
Alexa Fluor 700	NBP2-74356AF700
Alexa Fluor 750	NBP2-74356AF750
Allophycocyanin	NBP2-74356APC
Biotin	NBP2-74356B
CoraFluor 1	NBP2-74356CL1
DyLight 350	NBP2-74356UV
DyLight 405	NBP2-74356V
DyLight 488	NBP2-74356G
DyLight 550	NBP2-74356R
DyLight 594	NBP2-74356DL594
DyLight 650	NBP2-74356C
DyLight 680	NBP2-74356FR
DyLight 755	NBP2-74356IR
FITC	NBP2-74356F
HRP	NBP2-74356H
Janelia Fluor 525	NBP2-74356JF525
Janelia Fluor 549	NBP2-74356JF549
Janelia Fluor 646	NBP2-74356JF646
Janelia Fluor 669	NBP2-74356JF669
PE	NBP2-74356PE
PerCP	NBP2-74356PCP
mFluor Violet 450 SE	NBP2-74356MFV450
mFluor Violet 500 SE	NBP2-74356MFV500
mFluor Violet 610 SE	NBP2-74356MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-74356

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NBP1-47695

Chk2 Antibody (OTI5C4)

0.1 ml

NBP1-47695

Immunohistochemistry-Paraffin: Chk2 Antibody (OTI5C4) [NBP1-47695] - Staining of paraffin-embedded Human pancreas tissue using anti-Chk2 mouse monoclonal antibody.

Flow Cytometry: Chk2 Antibody (OTI5C4) [NBP1-47695] - Analysis of Jurkat cells, using anti-Chk2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Western Blot: Chk2 Antibody (OTI5C4) [NBP1-47695] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-Chk2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemistry-Paraffin: Chk2 Antibody (OTI5C4) [NBP1-47695] - Staining of paraffin-embedded Human breast tissue using anti-Chk2 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Chk2 Antibody (OTI5C4) [NBP1-47695] - Staining of paraffin-embedded Human colon tissue using anti-Chk2 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Chk2 Antibody (OTI5C4) [NBP1-47695] - Staining of paraffin-embedded Human Kidney tissue using anti-Chk2 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Chk2 Antibody (OTI5C4) [NBP1-47695] - Staining of paraffin-embedded Human liver tissue using anti-Chk2 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Chk2 Antibody (OTI5C4) [NBP1-47695] - Staining of paraffin-embedded Human lung tissue using anti-Chk2 mouse monoclonal antibody.

Reactivity:

Hu, Mu, Rt, Ca

Species Glossary

Reactivity: Hu, Mu, Rt, Ca

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-71688AF350
Alexa Fluor 405	NBP2-71688AF405
Alexa Fluor 488	NBP2-71688AF488
Alexa Fluor 532	NBP2-71688AF532
Alexa Fluor 594	NBP2-71688AF594
Alexa Fluor 647	NBP2-71688AF647
Alexa Fluor 700	NBP2-71688AF700
Alexa Fluor 750	NBP2-71688AF750
Allophycocyanin	NBP2-71688APC
Biotin	NBP2-71688B
CoraFluor 1	NBP2-71688CL1
DyLight 350	NBP2-71688UV
DyLight 405	NBP2-71688V
DyLight 488	NBP2-71688G
DyLight 550	NBP2-71688R
DyLight 594	NBP2-71688DL594
DyLight 650	NBP2-71688C
DyLight 680	NBP2-71688FR
DyLight 755	NBP2-71688IR
FITC	NBP2-71688F
HRP	NBP2-71688H
Janelia Fluor 525	NBP2-71688JF525
Janelia Fluor 549	NBP2-71688JF549
Janelia Fluor 646	NBP2-71688JF646
Janelia Fluor 669	NBP2-71688JF669
PE	NBP2-71688PE
PerCP	NBP2-71688PCP
mFluor Violet 450 SE	NBP2-71688MFV450
mFluor Violet 500 SE	NBP2-71688MFV500
mFluor Violet 610 SE	NBP2-71688MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-71688

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NB100-774

$Validation of R-Ras2 as the defatty-acylation target of SIRT6.(A) mRNA and protein levels of R-Ras2 in Sirt6 WT and KO MEFs. (B) In-gel fluorescence (with NH2OH treatment) showing that R-Ras2 has higher lysine fatty acylation level in Sirt6 KO MEFs than in Sirt6 WT MEFs. Right histogram shows the quantification of bands on the fluorescence gel. Values with error bars indicate mean ± s.d. of three biological replicates. * indicates p<0.05. The full fluorescence gel is shown in Figure 2—figure supplement 2A. (C) Detection of R-Ras2 lysine fatty acylation levels in control and SIRT6 knockown HEK 293 T cells by in-gel fluorescence. Right histogram shows the quantification of bands on the fluorescence gel. Values with error bars indicate mean ± s.d. of three biological replicates. * indicates p<0.05. (D) Lysine fatty acylation levels of endogenous R-Ras2 in Sirt6 WT and KO MEFs. (E, F) SIRT6 defatty-acylated R-Ras2 in a NAD+-dependent manner in vitro. In-gel fluorescence was used to detect R-Ras2 lysine fatty acylation (E). A 32P-NAD+ assay was used to detect fatty acyl ADPR product from defatty-acylation reaction. (F). (G) In-gel fluorescence (with NH2OH treatment) showing that mutation of four lysine residues at the C-terminus of R-Ras2 significantly decreased lysine fatty acylation in Sirt6 KO MEFs. Right histogram shows the quantification of bands on the fluorescence gel. Values with error bars indicate mean ± s.d. of three biological replicates. * indicates p<0.05. The full fluorescence gel including R-Ras2 total fatty acylation levels (without NH2OH treatment) is shown in Figure 2—figure supplement 2C. (H) Tandem mass (MS/MS) spectrum of doubly charged Alk14 modified (on K194) R-Ras2 peptide. The b- and y- ions are shown along with the peptide sequence. (I) In-gel fluorescence (with NH2OH treatment) showing that single mutation of four lysine residues at the C-terminus of R-Ras2 did not affect R-Ras2 lysine fatty acylation. (J) Confocal imaging showed that R-Ras2 WT was mainly localized in the plasma membrane in Sirt6 KO MEFs. R-Ras2 WT in Sirt6 WT MEFs as well as R-Ras2 4KR in Sirt6 WT and KO MEFs was localized in both the intracellular vesicles and plasma membrane (n = 5, 5, 5, 6 cells for each sample from left to right, respectively. The images of other cells were shown in Figure 2—figure supplement 4A.DOI://dx.doi.org/10.7554/eLife.25158.005Scheme showing the in-gel fluorescence method with Alk14 metabolic labeling to identify R-Ras2 as a lysine fatty acylated protein.FLAG-tagged R-Ras2 protein was enriched from whole cell lysates by FlAG immunoprecipitation. Alk14-labeled R-Ras2 protein was detected by in-gel fluorescence after incorporating BODIPY (B)-azide using click chemistry.DOI://dx.doi.org/10.7554/eLife.25158.006Validation of R-Ras2 as the defatty-acylation target of SIRT6. (A) Full gel image of R-Ras2 fatty acylation level with or without NH2OH treatment in Sirt6 WT and KO MEFs. (B) Lysine fatty acylation levels of overexpressed R-Ras2 in Sirt6 WT and KO MEFs. (C) Full gel image of R-Ras2 WT and 4KR fatty acylation levels with or without NH2OH treatment in Sirt6 KO MEFs. (D) In-gel fluorescence showing lysine fatty acylation level of overexpressed R-Ras2 WT and C199S mutant in HEK 293T cells with SIRT6 knockdown. C, control shRNA. S6, SIRT6 shRNA#1.DOI://dx.doi.org/10.7554/eLife.25158.007Total ion chromatogram (TIC), extracted ion chromatogram (XIC) and parent MS (MS1) of Ak14 modified R-Ras2 peptide.DOI://dx.doi.org/10.7554/eLife.25158.008Lysine fatty acylation targets R-Ras2 to plasma membrane.(A) Confocal imaging showing subcellular localization of GFP-tagged R-Ras2 WT and 4KR in Sirt6 WT and KO MEFs. (B) Subcellular fractionation of R-Ras2 WT and 4KR in HEK 293T cells with palmitic acid or TM3 treatment. GAPDH was used as the marker of cytosol fraction and Na,K-APTase was used as the marker of plasma membrane fraction.DOI://dx.doi.org/10.7554/eLife.25158.009 Image collected and cropped by CiteAb from the following open publication (//elifesciences.org/articles/25158), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Reactivity:

Hu, Mu, Rt, Po, Bv, Ca

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ca

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP2-52424

BMP-4 Antibody (3C11C7) - BSA Free

(2 Publications)

0.1 mg

NBP2-52424

Flow Cytometry: BMP-4 Antibody (3C11C7) [NBP2-52424] - Flow cytometric analysis of Hela cells using BMP4 mouse mAb (green) and negative control (red).

Western Blot: BMP-4 Antibody (3C11C7) [NBP2-52424] - Analysis using BMP4 mAb against HEK293 (1) and BMP4 (AA: 277-408)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot: BMP-4 Antibody (3C11C7) [NBP2-52424] - Analysis using BMP4 mAb against human BMP4 recombinant protein. (Expected MW is 41 kDa)

Immunocytochemistry/Immunofluorescence: BMP-4 Antibody (3C11C7) [NBP2-52424] - Immunofluorescence analysis of Hela cells using BMP4 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

ELISA: BMP-4 Antibody (3C11C7) [NBP2-52424] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Reactivity:

Hu, Rt

Species Glossary

Reactivity: Hu, Rt

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-52424-0.025mg

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NBP2-22204

BMI-1 Antibody (3E3)

(2 Publications)

0.1 ml

NBP2-22204

Immunohistochemistry-Paraffin: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis of paraffin-embedded rectum cancer tissues using Bmi1 mouse mAb with DAB staining.

Flow Cytometry: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis of NIH/3T3 cells using Bmi1 mouse mAb (green) and negative control (red).

Western Blot: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis using Bmi1 mAb against HEK293 (1) and BMI1-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemistry-Paraffin: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis of paraffin-embedded cervical cancer tissues using Bmi1 mouse mAb with DAB staining.

ELISA: BMI-1 Antibody (3E3) [NBP2-22204] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP1-97563

Integrin beta 3/CD61 Antibody (VIPL2) - BSA Free

(2 Publications)

0.1 mg

NBP1-97563

Flow Cytometry: CD41/CD61 Antibody (VIPL2) [NBP1-97563] - Staining of human peripheral blood with anti-human CD61 (VIPL2) APC.

Flow Cytometry: CD41/CD61 Antibody (VIPL2) [NBP1-97563] - Staining of human peripheral blood with anti-human CD61 (VIPL2) APC-CyTM7.

Flow Cytometry: CD41/CD61 Antibody (VIPL2) [NBP1-97563] - Staining of human peripheral blood with anti-human CD61 (VIPL2) PE.

Flow Cytometry: CD41/CD61 Antibody (VIPL2) [NBP1-97563] - Staining of human peripheral blood with anti-human CD61 (VIPL2) PerCP.

Flow Cytometry: CD41/CD61 Antibody (VIPL2) [NBP1-97563] - Staining of human peripheral blood with anti-human CD61 (VIPL2) FITC.

Separation of human CD61 positive thrombocytes (red-filled) from neutrophil granulocytes (black-dashed) in Analysis (surface staining) of peripheral whole blood stained using anti-human CD61 (VIPL2) purified antibody (concentration in sample 3 ug/ml, GAM APC).

Reactivity:

Hu, Pm

Species Glossary

Reactivity: Hu, Pm

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-97563AF350
Alexa Fluor 405	NBP1-97563AF405
Alexa Fluor 488	NBP1-97563AF488
Alexa Fluor 532	NBP1-97563AF532
Alexa Fluor 594	NBP1-97563AF594
Alexa Fluor 647	NBP1-97563AF647
Alexa Fluor 700	NBP1-97563AF700
Alexa Fluor 750	NBP1-97563AF750
Allophycocyanin	NBP1-97563APC
Allophycocyanin/Cy7	NBP1-97563APCCY7
Biotin	NBP1-97563B
CoraFluor 1	NBP1-97563CL1
DyLight 350	NBP1-97563UV
DyLight 405	NBP1-97563V
DyLight 488	NBP1-97563G
DyLight 550	NBP1-97563R
DyLight 594	NBP1-97563DL594
DyLight 650	NBP1-97563C
DyLight 680	NBP1-97563FR
DyLight 755	NBP1-97563IR
FITC	NBP1-97563F
HRP	NBP1-97563H
Janelia Fluor 525	NBP1-97563JF525
Janelia Fluor 549	NBP1-97563JF549
Janelia Fluor 646	NBP1-97563JF646
Janelia Fluor 669	NBP1-97563JF669
PE	NBP1-97563PE
PE/Atto594	NBP1-97563PEATT594
PE/Cy5.5	NBP1-97563PECY55
PE/Cy7	NBP1-97563PECY7
PerCP	NBP1-97563PCP
mFluor Violet 450 SE	NBP1-97563MFV450
mFluor Violet 500 SE	NBP1-97563MFV500
mFluor Violet 610 SE	NBP1-97563MFV610

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NBP2-67292

VCAM-1/CD106 Antibody (SA05-04)

(1 Publication)

100 ul

NBP2-67292

Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (SA05-04) [NBP2-67292] - Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-VCAM-1/CD106 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA

Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (SA05-04) [NBP2-67292] - Analysis of paraffin-embedded human spleen tissue using anti-VCAM1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (SA05-04) [NBP2-67292] - Analysis of paraffin-embedded human tonsil tissue using anti-VCAM1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (SA05-04) [NBP2-67292] - Analysis of paraffin-embedded mouse spleen tissue using anti-VCAM1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (SA05-04) [NBP2-67292] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-VCAM-1/CD106 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA

Reactivity:

Hu, Mu, Rt, Ze

Species Glossary

Reactivity: Hu, Mu, Rt, Ze

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NBP2-47916

Moesin Antibody (MSN/492) - Azide and BSA Free

0.1 mg

NBP2-47916

Immunohistochemistry-Paraffin: Moesin Antibody (MSN/492) - Azide and BSA Free [NBP2-47916] - Human Melanoma stained with Moesin Monoclonal Antibody (MSN/492)

Flow Cytometry: Moesin Antibody (MSN/492) - Azide and BSA Free [NBP2-47916] - Flow Cytometric Analysis of K562 cells. Moesin Antibody (MSN/492) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Western Blot: Moesin Antibody (MSN/492) - Azide and BSA Free [NBP2-47916] - Western Blot Analysis of human Jurkat cell lysate using Moesin Antibody (MSN/492).

Immunohistochemistry-Paraffin: Moesin Antibody (MSN/492) - Azide and BSA Free [NBP2-47916] - Human Testicular Carcinoma stained with Moesin Monoclonal Antibody (MSN/492)

Immunohistochemistry-Paraffin: Moesin Antibody (MSN/492) - Azide and BSA Free [NBP2-47916] - Human Placenta stained with Moesin Monoclonal Antibody (MSN/492)

Reactivity:

Hu, Rt(-)

Species Glossary

Reactivity: Hu, Rt(-)

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-47916AF350
Alexa Fluor 405	NBP2-47916AF405
Alexa Fluor 488	NBP2-47916AF488
Alexa Fluor 532	NBP2-47916AF532
Alexa Fluor 594	NBP2-47916AF594
Alexa Fluor 647	NBP2-47916AF647
Alexa Fluor 700	NBP2-47916AF700
Alexa Fluor 750	NBP2-47916AF750
Allophycocyanin	NBP2-47916APC
Allophycocyanin/Cy7	NBP2-47916APCCY7
Biotin	NBP2-47916B
CoraFluor 1	NBP2-47916CL1
DyLight 350	NBP2-47916UV
DyLight 405	NBP2-47916V
DyLight 488	NBP2-47916G
DyLight 550	NBP2-47916R
DyLight 594	NBP2-47916DL594
DyLight 650	NBP2-47916C
DyLight 680	NBP2-47916FR
DyLight 755	NBP2-47916IR
FITC	NBP2-47916F
HRP	NBP2-47916H
Janelia Fluor 525	NBP2-47916JF525
Janelia Fluor 549	NBP2-47916JF549
Janelia Fluor 585	NBP2-47916JF585
Janelia Fluor 635	NBP2-47916JF635
Janelia Fluor 646	NBP2-47916JF646
Janelia Fluor 669	NBP2-47916JF669
PE	NBP2-47916PE
PE/Atto594	NBP2-47916PEATT594
PE/Cy5.5	NBP2-47916PECY55
PE/Cy7	NBP2-47916PECY7
PerCP	NBP2-47916PCP
mFluor Violet 450 SE	NBP2-47916MFV450
mFluor Violet 500 SE	NBP2-47916MFV500
mFluor Violet 610 SE	NBP2-47916MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
IHC-Prediluted	NBP2-48305

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Marker

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Kit Type

Lysate Type

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

MBP Antibody (CL2819) - BSA Free

CDKN3 Antibody (OTI2E11)

LIN-28A Antibody (14E6-4E6)

Fumarase Antibody (OTI1F10)

SSB Antibody (OTI2C8)

Chk2 Antibody (OTI5C4)

PGAM1/2/4 Antibody

BMP-4 Antibody (3C11C7) - BSA Free

BMI-1 Antibody (3E3)

Integrin beta 3/CD61 Antibody (VIPL2) - BSA Free

VCAM-1/CD106 Antibody (SA05-04)

Moesin Antibody (MSN/492) - Azide and BSA Free