| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89557-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92192-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70963 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-79127-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-21803-0.025ml | |||
![Immunohistochemistry-Paraffin: CLPP Antibody [NBP1-89557] - Staining of human small intestine shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/CLPP-Antibody-Immunohistochemistry-Paraffin-NBP1-89557-img0015.jpg "Immunohistochemistry-Paraffin: CLPP Antibody [NBP1-89557] - Staining of human small intestine shows strong granular cytoplasmic positivity in glandular cells.")
![Immunohistochemistry-Paraffin: CLPP Antibody [NBP1-89557] - Staining of human kidney shows moderate granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/CLPP-Antibody-Immunohistochemistry-Paraffin-NBP1-89557-img0012.jpg)
![Immunohistochemistry-Paraffin: CLPP Antibody [NBP1-89557] - Staining of human liver shows moderate granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/CLPP-Antibody-Immunohistochemistry-Paraffin-NBP1-89557-img0013.jpg)
![Immunohistochemistry-Paraffin: CLPP Antibody [NBP1-89557] - Staining of human pancreas shows moderate granular cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/CLPP-Antibody-Immunohistochemistry-Paraffin-NBP1-89557-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: Borealin Antibody [NBP1-89951] - Staining of human cell line U-251 MG shows localization to nucleus & nucleoli. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Borealin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89951-img0010.jpg "Immunocytochemistry/Immunofluorescence: Borealin Antibody [NBP1-89951] - Staining of human cell line U-251 MG shows localization to nucleus & nucleoli. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Borealin Antibody [NBP1-89951] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Borealin-Antibody-Immunohistochemistry-Paraffin-NBP1-89951-img0015.jpg)
![Immunohistochemistry-Paraffin: Borealin Antibody [NBP1-89951] - Staining of human duodenum shows strong nuclear positivity in a subset of glandular cells.](http://images.novusbio.com/fullsize/Borealin-Antibody-Immunohistochemistry-Paraffin-NBP1-89951-img0012.jpg)
![Immunohistochemistry-Paraffin: Borealin Antibody [NBP1-89951] - Staining of human lymphoid tissues shows moderate nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/Borealin-Antibody-Immunohistochemistry-Paraffin-NBP1-89951-img0013.jpg)
![Western Blot: Borealin Antibody [NBP1-89951] - Aurora B preferentially binds to H3R2me2a & recruits CPC components.a The amino acid sequences of modified histone H3 peptides. b In vitro Haspin kinase assay w/ 21-aa peptide. c In vitro Aurora B kinase assay w/ 21-aa peptide for phosphorylation. d In vitro PRMT6 methyltransferase assay. e–h The cells treated w/ siPRMT6, PRMT6 inhibitor MS023, or the Haspin kinase inhibitor CHR-6494. The intensities of H3R2me2a (f) & H3T3ph (h) in chromosome arm & centromere analyzed by IF microscopy of nocodazole-arrested HeLa chromosome spreads & plotted (n = 100 chromosomes from three independent experiments). i After transfection of indicated plasmids, HeLa cells treated w/ a Haspin kinase inhibitor, CHR-6494, for 5 h & the intensity of CPC components analyzed for 100 centromeres from three independent experiments. j Recombinant CPC proteins incubated w/ biotinylated histone peptides & pulled down w/ streptavidin-agarose beads, & the binding visualized by immunoblotting. k Lysates of TN-arrested Aurora B-depleted cells supplemented w/ recombinant Survivin & Borealin proteins w/ or w/out recombinant Aurora B protein. The lysates incubated w/ antibodies against INCENP & the H3R2me2a peptide, & pulldown conducted w/ agarose A beads. The binding visualized by immunoblotting. The asterisk denotes the light chain of antibodies. l Lysates of TN-arrested mitotic HeLa cells incubated w/ biotinylated histone peptides & pulled down w/ streptavidin-agarose beads, & the binding visualized by immunoblotting. Relative band intensities of band measured w/ image processing software (Image Studio ver5.0). Error bars, SEMs. Scale bars, 5 μm. Source data are provided as a Source Data file. (Student’s t-test *p < 0.01). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32001712), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-89951_rabbit-polyclonal-borealin-antibody-310202416113528.jpg)
![Western Blot: Borealin Antibody [NBP1-89951] - Aurora B preferentially binds to H3R2me2a & recruits CPC components.a The amino acid sequences of modified histone H3 peptides. b In vitro Haspin kinase assay w/ 21-aa peptide. c In vitro Aurora B kinase assay w/ 21-aa peptide for phosphorylation. d In vitro PRMT6 methyltransferase assay. e–h The cells treated w/ siPRMT6, PRMT6 inhibitor MS023, or the Haspin kinase inhibitor CHR-6494. The intensities of H3R2me2a (f) & H3T3ph (h) in chromosome arm & centromere analyzed by IF microscopy of nocodazole-arrested HeLa chromosome spreads & plotted (n = 100 chromosomes from three independent experiments). i After transfection of indicated plasmids, HeLa cells treated w/ a Haspin kinase inhibitor, CHR-6494, for 5 h & the intensity of CPC components analyzed for 100 centromeres from three independent experiments. j Recombinant CPC proteins incubated w/ biotinylated histone peptides & pulled down w/ streptavidin-agarose beads, & the binding visualized by immunoblotting. k Lysates of TN-arrested Aurora B-depleted cells supplemented w/ recombinant Survivin & Borealin proteins w/ or w/out recombinant Aurora B protein. The lysates incubated w/ antibodies against INCENP & the H3R2me2a peptide, & pulldown conducted w/ agarose A beads. The binding visualized by immunoblotting. The asterisk denotes the light chain of antibodies. l Lysates of TN-arrested mitotic HeLa cells incubated w/ biotinylated histone peptides & pulled down w/ streptavidin-agarose beads, & the binding visualized by immunoblotting. Relative band intensities of band measured w/ image processing software (Image Studio ver5.0). Error bars, SEMs. Scale bars, 5 μm. Source data are provided as a Source Data file. (Student’s t-test *p < 0.01). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32001712), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-89951_rabbit-polyclonal-borealin-antibody-310202416121943.jpg)
![Immunocytochemistry/Immunofluorescence: NOL1 Antibody [NBP1-92192] - Staining of human cell line A-431 shows localization to nucleoli. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NOL1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-92192-img0012.jpg "Immunocytochemistry/Immunofluorescence: NOL1 Antibody [NBP1-92192] - Staining of human cell line A-431 shows localization to nucleoli. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: NOL1 Antibody [NBP1-92192] - Staining of human skin shows distinct nucleolar positivity in keratinocytes.](http://images.novusbio.com/fullsize/NOL1-Antibody-Immunohistochemistry-Paraffin-NBP1-92192-img0014.jpg)
![Western Blot: NOL1 Antibody [NBP1-92192] - Analysis in human cell line NTERA-2.](http://images.novusbio.com/fullsize/NOL1-Antibody-Western-Blot-NBP1-92192-img0010.jpg)
![Western Blot: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Analysis of extracts from 9 different cell lines: (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-9F8-Western-Blot-NBP1-47801-img0022.jpg "Western Blot: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Analysis of extracts from 9 different cell lines: (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunocytochemistry/Immunofluorescence: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY GRP75/HSPA9B/Mortalin.](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-9F8-Immunocytochemistry-Immunofluorescence-NBP1-47801-img0014.jpg)
![Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Staining of paraffin-embedded Human pancreas tissue using anti-GRP75/HSPA9B/Mortalin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-9F8-Immunohistochemistry-Paraffin-NBP1-47801-img0020.jpg)
![Flow Cytometry: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Analysis of Jurkat cells, using anti-Mortalin antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-9F8-Flow-Cytometry-NBP1-47801-img0013.jpg)
![Western Blot: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRP75/HSPA9B/Mortalin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRP75/HSPA9B/Mortalin.](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-9F8-Western-Blot-NBP1-47801-img0021.jpg)
![Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-GRP75/HSPA9B/Mortalin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-9F8-Immunohistochemistry-Paraffin-NBP1-47801-img0015.jpg)
![Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-GRP75/HSPA9B/Mortalin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-9F8-Immunohistochemistry-Paraffin-NBP1-47801-img0016.jpg)
![Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-GRP75/HSPA9B/Mortalin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-9F8-Immunohistochemistry-Paraffin-NBP1-47801-img0017.jpg)
![Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-GRP75/HSPA9B/Mortalin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-9F8-Immunohistochemistry-Paraffin-NBP1-47801-img0018.jpg)
![Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Staining of paraffin-embedded Human endometrium tissue using anti-GRP75/HSPA9B/Mortalin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-9F8-Immunohistochemistry-Paraffin-NBP1-47801-img0019.jpg)
![Flow Cytometry: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Analysis of Hela cells, using anti-Mortalin antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-9F8-Flow-Cytometry-NBP1-47801-img0023.jpg)
![Western Blot: TRAP1 Antibody (TRAP1-6) [NB300-555] - Figure 2 illustrates IP of TRAP1 visualized by Coomassie Blue staining.](http://images.novusbio.com/fullsize/TRAP1-Antibody-TRAP1-6-Western-Blot-NB300-555-img0005.jpg "Western Blot: TRAP1 Antibody (TRAP1-6) [NB300-555] - Figure 2 illustrates IP of TRAP1 visualized by Coomassie Blue staining.")
![Immunocytochemistry/Immunofluorescence: TRAP1 Antibody (TRAP1-6) [NB300-555] - Analysis of TRAP1 in NCI-H460 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a TRAP1 monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. TRAP1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/TRAP1-Antibody-TRAP1-6-Immunocytochemistry-Immunofluorescence-NB300-555-img0006.jpg)
![Immunohistochemistry-Paraffin: TRAP1 Antibody (TRAP1-6) [NB300-555] - Normal biopsies of deparaffinized Human liver tissue, dilution of 1:20.](http://images.novusbio.com/fullsize/TRAP1-Antibody-TRAP1-6-Immunohistochemistry-Paraffin-NB300-555-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: TRAP1 Antibody (TRAP1-6) [NB300-555] - Immunolocalization of TRAP1 in PC-3-M cells.](http://images.novusbio.com/fullsize/TRAP1-Antibody-TRAP1-6-Immunocytochemistry-Immunofluorescence-NB300-555-img0003.jpg)
![Immunohistochemistry: TRAP1 Antibody (TRAP1-6) [NB300-555] - Representative images of IHF staining of EP2 or EP4 (green) and TRAP1 (NB300-555) (red) in subchondral bone 2 weeks post-sham or ACLT surgery (left) and quantitative analysis (right). The white arrows indicate TRAP-positive osteoclasts expressing EP2 or EP4. Scale bars, 20 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35260562/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nb300-555_mouse-monoclonal-trap1-antibody-trap1-6-25520231539518.jpg)
![Immunocytochemistry/ Immunofluorescence: TRAP1 Antibody (TRAP1-6) [NB300-555] - PGE2 regulates migration & osteoclast differentiation through the G alpha s/PI3K/AKT/MAPK signaling pathways downstream of EP4. PGE2 regulates migration & osteoclast differentiation independent of beta -arrestin1 (a) & beta -arrestin2 (b) but via G alpha s (c). BMMs from the WT & littermate beta -arrestin1- & beta -arrestin2-knockout mice were used to generate osteoclasts by stimulation with 10 ng·mL−1 M-CSF & 50 ng·mL−1 RANKL & incubation with 100 nmol·L−1 PGE2. For G alpha s experiments, osteoclasts were generated from the GMMs of the G alpha sfl/fl controls & G alpha sfl/fl; LysM-cre (G alpha sLysM) mice. A differentiation assay (TRAP staining) (left) & the corresponding quantitative analysis (right). Scale bars, 50 μm. Error bars are the mean ± s.d. n = 3. Two-way ANOVA followed by Tukey’s t tests. Scale bars, 50 μm. d cAMP production was measured via ELISAs for osteoclasts derived from the Ep4fl/fl & Ep4LysM mice. BMMs from the two mouse strains were isolated & stimulated with 10 ng·mL−1 M-CSF & 50 ng·mL−1 RANKL to differentiate into osteoclasts & incubated with 100 nmol·L−1 PGE2 for 30 min prior to cAMP measurements. Error bars are the mean ± s.d. ***P < 0.001 by unpaired two-tailed Student’s t test. The experiment was performed with three biological replicates. e Representative images of the indicated protein expression by western blotting for osteoclasts generated using BMMs of Ep4fl/fl & Ep4LysM mice. The cells were treated either with osteoclastogenic media (10 ng·mL−1 M-CSF & 50 ng·mL−1 RANKL) alone, with PGE2 (100 nmol·L−1), or PGE2 with IBMX (1 mmol·L−1) for 3 h. The experiments were performed with three biological replicates Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35260562), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-555_mouse-monoclonal-trap1-antibody-trap1-6-310202416212330.jpg)
![Western Blot: Presenilin-1 Antibody (APS 11) [NB300-749] - Analysis of 25 ug of SH-SY5Y (lane 1), human brain (lane 2) and mouse brain (lane 3).](http://images.novusbio.com/fullsize/Presenilin-1-Antibody-APS-11-Western-Blot-NB300-749-img0007.jpg "Western Blot: Presenilin-1 Antibody (APS 11) [NB300-749] - Analysis of 25 ug of SH-SY5Y (lane 1), human brain (lane 2) and mouse brain (lane 3).")
![Immunocytochemistry/Immunofluorescence: Presenilin-1 Antibody (APS 11) [NB300-749] - Mouse fibroblasts.](http://images.novusbio.com/fullsize/Presenilin-1-Antibody-APS-11-Immunocytochemistry-Immunofluorescence-NB300-749-img0004.jpg)
![Immunohistochemistry-Paraffin: Presenilin-1 Antibody (APS 11) [NB300-749] - Immunohistochemistry was performed on normal biopsies of deparaffinized Human liver tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Presenilin 1 or without primary antibody (negative control) overnight at 4C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Tissues were counterstained with hematoxylin and prepped for mounting.](http://images.novusbio.com/fullsize/Presenilin-1-Antibody-APS-11-Immunohistochemistry-Paraffin-NB300-749-img0006.jpg)
![Immunohistochemistry-Paraffin: Presenilin-1 Antibody (APS 11) [NB300-749] - Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing Presenilin 1 or without primary antibody (negative control) overnight at 4C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Tissues were counterstained with hematoxylin and prepped for mounting.](http://images.novusbio.com/fullsize/Presenilin-1-Antibody-APS-11-Immunohistochemistry-Paraffin-NB300-749-img0005.jpg)
![Flow Cytometry: CD45 Antibody (HI30) [NBP1-79127] - Surface staining of human peripheral blood cells with anti-human CD45 antibody (HI30) [NBP1-79127] Pacific BlueTM.](http://images.novusbio.com/fullsize/CD45-Antibody-HI30-Flow-Cytometry-NBP1-79127-img0007.jpg "Flow Cytometry: CD45 Antibody (HI30) [NBP1-79127] - Surface staining of human peripheral blood cells with anti-human CD45 antibody (HI30) [NBP1-79127] Pacific BlueTM.")
![Flow (Cell Surface): CD45 Antibody (HI30) [NBP1-79127] - hPBMC cells were stained with CD45 antibody (HI30) [NBP1-79127] (blue) and a matched isotype control [NBP2-27287] (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. The antibodies were directly conjugated to Alexa Flour 488.](http://images.novusbio.com/fullsize/CD45-Antibody-HI30-Flow-Cell-Surface-NBP1-79127-img0005.jpg)
![Flow Cytometry: CD45 Antibody (HI30) [NBP1-79127] - Surface staining of human peripheral blood cells with anti-human CD45 antibody (HI30) [NBP1-79127] Pacific OrangeTM.](http://images.novusbio.com/fullsize/CD45-Antibody-HI30-Flow-Cytometry-NBP1-79127-img0008.jpg)
![Flow Cytometry: CD45 Antibody (HI30) [NBP1-79127] - Surface staining of human peripheral blood cells with anti-human CD45 antibody (HI30) [NBP1-79127] purified, GAM-APC.](http://images.novusbio.com/fullsize/CD45-Antibody-HI30-Flow-Cytometry-NBP1-79127-img0009.jpg)
![Flow Cytometry: CD45 Antibody (HI30) [NBP1-79127] - Analysis of PE/Cy7 conjugate of CD45 antibody (HI30) [NBP1-79127]. Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control PE-Cy7 (blue histogram) or Anti-Human CD45 PE-Cy7 (purple histogram). Cells in the lymphocyte gate were used for analysis.](http://images.novusbio.com/fullsize/CD45-Antibody-HI30-Flow-Cytometry-NBP1-79127-img0010.jpg)
![Flow (Cell Surface): CD45 Antibody (HI30) [NBP1-79127] - Flow (Cell Surface): Cell surface staining of PBMC's (Lymphocyte gate at 1 x 10^6 cells/ml) with CD45 antibody (HI30) [Alexa Fluor (R) 647] [NBP1-79127AF647] (orange) stained at a dilution of 1:500. Shown with mIgG1 (AF647 conjugated) Isotype control (blue). Image using the Alexa Fluor 647 form of this antibody.](http://images.novusbio.com/fullsize/CD45-Antibody-HI30-Flow-Cell-Surface-NBP1-79127-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Glucagon Antibody (09) [NBP2-21803] - Staining in human pancreas tissueSignal is strong in alpha cells. Dilution: 1:4000](http://images.novusbio.com/fullsize/Glucagon-Antibody-09-Immunocytochemistry-Immunofluorescence-NBP2-21803-img0001.jpg "Immunocytochemistry/Immunofluorescence: Glucagon Antibody (09) [NBP2-21803] - Staining in human pancreas tissueSignal is strong in alpha cells. Dilution: 1:4000")
![Immunohistochemistry-Paraffin: Glucagon Antibody (09) [NBP2-21803] - Staining of human pancreas tissue . Alpha cells of the Langerhans islet were stained strongly. Dilution: 1:8000](http://images.novusbio.com/fullsize/Glucagon-Antibody-09-Immunohistochemistry-Paraffin-NBP2-21803-img0002.jpg)
![Sandwich ELISA: Glucagon Antibody (09) - BSA Free [NBP2-21803] - Glucagon Antibody (09) [NBP2-21803] - The calibration curve of a sandwich assay for Glucagon using NBP2-21803 as the capture antibody and NBP2-21802B as the biotinylated detection antibody.](http://images.novusbio.com/fullsize/Glucagon-Antibody-09-Sandwich-ELISA-NBP2-21803-img0009.jpg)
![Western Blot: CD14 Antibody (SC69-02) [NBP2-67630] - Analysis of CD14 on NIH/3T3 cell lysates with Rabbit anti-CD14 antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 40 kDa Observed band size: 65 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/CD14-Antibody-SC69-02-Western-Blot-NBP2-67630-img0012.jpg "Western Blot: CD14 Antibody (SC69-02) [NBP2-67630] - Analysis of CD14 on NIH/3T3 cell lysates with Rabbit anti-CD14 antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 40 kDa Observed band size: 65 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: CD14 Antibody (SC69-02) [NBP2-67630] - Staining CD14 in NCCIT cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD14-Antibody-SC69-02-Immunocytochemistry-Immunofluorescence-NBP2-67630-img0010.jpg)
![Immunohistochemistry-Paraffin: CD14 Antibody (SC69-02) [NBP2-67630] - Analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-CD14 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD14-Antibody-SC69-02-Immunohistochemistry-Paraffin-NBP2-67630-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: CD14 Antibody (SC69-02) [NBP2-67630] - Staining CD14 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD14-Antibody-SC69-02-Immunocytochemistry-Immunofluorescence-NBP2-67630-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD14 Antibody (SC69-02) [NBP2-67630] - Staining CD14 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD14-Antibody-SC69-02-Immunocytochemistry-Immunofluorescence-NBP2-67630-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD14 Antibody (SC69-02) [NBP2-67630] - Staining CD14 in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD14-Antibody-SC69-02-Immunocytochemistry-Immunofluorescence-NBP2-67630-img0009.jpg)
![Immunohistochemistry-Paraffin: CD14 Antibody (SC69-02) [NBP2-67630] - Analysis of paraffin-embedded human colon cancer tissue using anti-CD14 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD14-Antibody-SC69-02-Immunohistochemistry-Paraffin-NBP2-67630-img0003.jpg)
![Immunohistochemistry-Paraffin: CD14 Antibody (SC69-02) [NBP2-67630] - Analysis of paraffin-embedded human liver tissue using anti-CD14 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD14-Antibody-SC69-02-Immunohistochemistry-Paraffin-NBP2-67630-img0004.jpg)
![Immunohistochemistry-Paraffin: CD14 Antibody (SC69-02) [NBP2-67630] - Analysis of paraffin-embedded human spleen tissue using anti-CD14 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD14-Antibody-SC69-02-Immunohistochemistry-Paraffin-NBP2-67630-img0005.jpg)
![Immunohistochemistry-Paraffin: CD14 Antibody (SC69-02) [NBP2-67630] - Analysis of paraffin-embedded human tonsil tissue using anti-CD14 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD14-Antibody-SC69-02-Immunohistochemistry-Paraffin-NBP2-67630-img0006.jpg)
![Immunohistochemistry-Paraffin: CD14 Antibody (SC69-02) [NBP2-67630] - Analysis of paraffin-embedded human uterus tissue using anti-CD14 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD14-Antibody-SC69-02-Immunohistochemistry-Paraffin-NBP2-67630-img0007.jpg)
![Immunohistochemistry-Paraffin: CD14 Antibody (SC69-02) [NBP2-67630] - Analysis of paraffin-embedded human liver tissue with Rabbit anti-CD14 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD14-Antibody-SC69-02-Immunohistochemistry-Paraffin-NBP2-67630-img0013.jpg)
![Immunohistochemistry-Paraffin: CD14 Antibody (SC69-02) [NBP2-67630] - Analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD14 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD14-Antibody-SC69-02-Immunohistochemistry-Paraffin-NBP2-67630-img0014.jpg)
![Western Blot: CD14 Antibody (SC69-02) [NBP2-67630] - analysis of CD14 on different lysates with Rabbit anti-CD14 antibody at 1/1,000 dilution.Lane 1: SW480-si NT cell lysateLane 2: SW480-si CD14 cell lysateLysates/proteins at 10 ug/Lane.Predicted band size: 40 kDaObserved band size: 50-60 kDaExposure time: 7 seconds;ECL: merk4-20% SDS-PAGE gel.](http://images.novusbio.com/fullsize/nbp2-67630_rabbit-monoclonal-cd14-antibody-sc69-02-western-blot-264202493347..jpg)
![Western Blot: CD14 Antibody (SC69-02) [NBP2-67630] - analysis of CD14 on different lysates with Rabbit anti-CD14 antibody at 1/5,000 dilution.Lane 1: THP-1 cell lysateLane 2: RAW264.7 cell lysateLysates/proteins at 15 ug/Lane.Predicted band size: 40 kDaObserved band size: 60 kDaExposure time: 1 minute 40 seconds;4-20% SDS-PAGE gel.](http://images.novusbio.com/fullsize/nbp2-67630_rabbit-monoclonal-cd14-antibody-sc69-02-western-blot-264202493452..jpg)
![Flow Cytometry: Endoglin/CD105 Antibody (MEM-226) [NB500-452] - A cell surface stain was performed on HeLa cells with NB500-452F (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to FITC.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MEM-226-Flow-Cytometry-NB500-452-img0009.jpg "Flow Cytometry: Endoglin/CD105 Antibody (MEM-226) [NB500-452] - A cell surface stain was performed on HeLa cells with NB500-452F (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to FITC.")
![Flow Cytometry: Endoglin/CD105 Antibody (MEM-226) [NB500-452] - Surface staining (mass cytometry) of hTERT cell line with anti-human CD105 (MEM-226) 143Nd. Gated on singlets.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MEM-226-Flow-Cytometry-NB500-452-img0001.jpg)
![Flow (Cell Surface): Endoglin/CD105 Antibody (MEM-226) [NB500-452] - Analysis using the FITC conjugate of NB500-452. Staining of HUVEC (human umbilical vein endothelial cells) with anti-human CD105 (MEM-226) FITC. Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MEM-226-Flow-Cell-Surface-NB500-452-img0003.jpg)
![Flow Cytometry: Endoglin/CD105 Antibody (MEM-226) [NB500-452] - Surface staining of HUVEC (human umbilical vein endothelial cells) with anti-CD105 (MEM-226) PE. HEK-293 cells used as a negative control.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MEM-226-Flow-Cytometry-NB500-452-img0004.jpg)
![Flow (Cell Surface): Endoglin/CD105 Antibody (MEM-226) [NB500-452] - A cell surface stain was performed on HeLa cells with Endoglin/CD105 Antibody (MEM-226) NB500-452PE (blue) and a matched isotype control(orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MEM-226-Flow-Cell-Surface-NB500-452-img0005.jpg)
![Flow Cytometry: Endoglin/CD105 Antibody (MEM-226) [NB500-452] - A cell surface stain was performed on HeLa cells with Endoglin/CD105 Antibody (MEM-226) NB500-452AF647 (blue) and a matched isotype control(orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MEM-226-Flow-Cytometry-NB500-452-img0006.jpg)
![Flow Cytometry: Endoglin/CD105 Antibody (MEM-226) [NB500-452] - A cell surface stain was performed on HeLa cells with Endoglin/CD105 Antibody (MEM-226) NB500-452PCP (blue) and a matched isotype control(orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to PerCP.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MEM-226-Flow-Cytometry-NB500-452-img0007.jpg)
![Western Blot: Integrin beta 1/CD29 Antibody (MEM-101A) [NB500-383] - Western Blotting analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using anti-Integrin beta 1 Antibody (MEM-101A). Lane 1: lysate of human PBL Lane 2: lysate of canine PBL Lane 3: lysate of porcine PBL.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-MEM-101A-Western-Blot-NB500-383-img0003.jpg "Western Blot: Integrin beta 1/CD29 Antibody (MEM-101A) [NB500-383] - Western Blotting analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using anti-Integrin beta 1 Antibody (MEM-101A). Lane 1: lysate of human PBL Lane 2: lysate of canine PBL Lane 3: lysate of porcine PBL.")
![Flow Cytometry: Integrin beta 1/CD29 Antibody (MEM-101A) [NB500-383] - Staining of human peripheral blood with anti-human CD29 (MEM-101A) PE.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-MEM-101A-Flow-Cytometry-NB500-383-img0004.jpg)
![Flow Cytometry: Integrin beta 1/CD29 Antibody (MEM-101A) [NB500-383] - Surface staining of human peripheral blood with anti-human CD29 (MEM-101A) APC conjugated.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-MEM-101A-Flow-Cytometry-NB500-383-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: MCM7 Antibody [NBP1-31350] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/MCM7-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31350-img0004.jpg "Immunocytochemistry/Immunofluorescence: MCM7 Antibody [NBP1-31350] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: MCM7 Antibody [NBP1-31350] - Paraffin-embedded Cal27 Xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/MCM7-Antibody-Immunohistochemistry-Paraffin-NBP1-31350-img0005.jpg)
![Chromatin Immunoprecipitation (ChIP): MCM7 Antibody [NBP1-31350] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit igG or anti-MCM7 antibody. The precipitated DNA was detected by PCR with primer set targeting to Myc promoter.](http://images.novusbio.com/fullsize/MCM7-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP1-31350-img0008.jpg)
![Immunoprecipitation: MCM7 Antibody [NBP1-31350] - MCM7 antibody [N2C2], Internal immunoprecipitates MCM7 protein in IP experiments. IP samples: HeLa whole cell extract. A. 40 ug HeLa whole cell extract. B. Control with 4 ug of preimmune Rabbit IgG. C. Immunoprecipitation of MCM7 protein by 4 ug MCM7 antibody [N2C2], Internal. 7.5 % SDS-PAGE. The immunoprecipitated MCM7 protein was detected by MCM7 antibody [N2C2], Internal diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/MCM7-Antibody-Immunoprecipitation-NBP1-31350-img0007.jpg)
![Western Blot: Rabbit Polyclonal MCM7 Antibody [NBP1-31350] - Whole cell lysates from MDA-MB-231 and SUM159 cells were loaded with 50 ug/lane.10% SDS-PAGE. MCM7 Antibody (NBP1-31350) was used for primary antibody: 1:1000, 4℃, overnight. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-31350_rabbit-polyclonal-mcm7-antibody-western-blot-212202416030..jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM7 antibody [N2C2], Internal (NBP1-31350) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31350_rabbit-polyclonal-mcm7-antibody-western-blot-1411202518243316.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM7 antibody [N2C2], Internal (NBP1-31350) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31350_rabbit-polyclonal-mcm7-antibody-western-blot-141120251843245.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM7 antibody [N2C2], Internal (NBP1-31350) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31350_rabbit-polyclonal-mcm7-antibody-western-blot-1411202518381712.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM7 antibody [N2C2], Internal (NBP1-31350) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31350_rabbit-polyclonal-mcm7-antibody-western-blot-1411202518273411.jpg)