| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-13078-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59933-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05122-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27087SS | |||
| Azide and BSA Free | NBP2-80673 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47761-0.1mg | |||
| IHC-Prediluted | NBP2-48397 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27115SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26167-50ul | |||
![Western Blot: Fas/TNFRSF6/CD95 Antibody (4F8D6) - BSA Free [NBP2-13078] - Figure 3: Western blot analysis using Fas/TNFRSF6/CD95 mouse monoclonal antibody against Hela (1), Jurkat (2) cell lysate.](http://images.novusbio.com/fullsize/Fas-TNFRSF6-CD95-Antibody-4F8D6-Western-Blot-NBP2-13078-img0003.jpg "Western Blot: Fas/TNFRSF6/CD95 Antibody (4F8D6) - BSA Free [NBP2-13078] - Figure 3: Western blot analysis using Fas/TNFRSF6/CD95 mouse monoclonal antibody against Hela (1), Jurkat (2) cell lysate.")
![Flow Cytometry: Fas/TNFRSF6/CD95 Antibody (4F8D6) - BSA Free [NBP2-13078] - Figure 4: Flow cytometric analysis of Hela cells using Fas/TNFRSF6/CD95 mouse monoclonal antibody (green) and negative control (red).](http://images.novusbio.com/fullsize/Fas-TNFRSF6-CD95-Antibody-4F8D6-Flow-Cytometry-NBP2-13078-img0004.jpg)
![Western Blot: Fas/TNFRSF6/CD95 Antibody (4F8D6) - BSA Free [NBP2-13078] - Figure 2: Western blot analysis using Fas/TNFRSF6/CD95 monoclonal antibody against human Fas/TNFRSF6/CD95 (AA: 87-278) recombinant protein. (Expected MW is 47.2 kDa)](http://images.novusbio.com/fullsize/Fas-TNFRSF6-CD95-Antibody-4F8D6-Western-Blot-NBP2-13078-img0002.jpg)
![ELISA: Fas Receptor/TNFRSF6/CD95 Antibody (4F8D6) [NBP2-13078] - Fas/TNFRSF6/CD95 Antibody (4F8D6) [NBP2-13078] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Fas-TNFRSF6-CD95-Antibody-4F8D6-ELISA-NBP2-13078-img0001.jpg)
![Western Blot: Lamin A + C Antibody (WL4G10) [NBP2-59933] - Lamin A + C (WL4G10) [NBP2-59933] - Whole cell lysates from HeLa and 293T cells were separated on SDS-PAGE gel, transferred to nitrocellulose membrane and stained with WL4G10 antibody. The membrane was blocked overnight in blocking buffer (5% nonfat dry milk resuspended in 0.1% Tween and 1X PBS). Both primary and secondary antibodies were diluted in blocking buffer. As expected, 293T cells express less of the lamin A isoform.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-WL4G10-Western-Blot-NBP2-59933-img0006.jpg "Western Blot: Lamin A + C Antibody (WL4G10) [NBP2-59933] - Lamin A + C (WL4G10) [NBP2-59933] - Whole cell lysates from HeLa and 293T cells were separated on SDS-PAGE gel, transferred to nitrocellulose membrane and stained with WL4G10 antibody. The membrane was blocked overnight in blocking buffer (5% nonfat dry milk resuspended in 0.1% Tween and 1X PBS). Both primary and secondary antibodies were diluted in blocking buffer. As expected, 293T cells express less of the lamin A isoform.")
![Immunohistochemistry-Paraffin: Lamin A + C Antibody (WL4G10) [NBP2-59933] - Analysis of formalin-fixed paraffin-embedded human gut. Following heat induced antigen retrieval, the sections were stained with mouse monoclonal anti-lamin A+C clone WL4G10 antibody (green). Nuclei were visualized with DAPI.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-WL4G10-Immunocytochemistry-Immunofluorescence-NBP2-59933-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (WL4G10) [NBP2-59933] - Confocal images of methanol fixed Hela cells that were immunofluorescently stained with mouse monoclonal WL4G10 antibody. The antibody works as well on cells fixed with 4% PFA.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-WL4G10-Immunocytochemistry-Immunofluorescence-NBP2-59933-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (WL4G10) [NBP2-59933] - The mouse monoclonal WL4G10 antibody recognizes human lamins A and C. Immortalized mouse adult LMNA null fibroblast stably expressing: (A) mEos2-lamin A or (B) mEos2- lamin C fused were fixed in 4% PFA and stained with WL4G10 antibody (magenta). Expression of the respective LMNA isoform is shown in green. The nuclei were counter-stained with Hoechst. Of note, the WL4G10 antibody does not recognize mouse lamins A and C.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-WL4G10-Immunocytochemistry-Immunofluorescence-NBP2-59933-img0003.jpg)
![Immunohistochemistry: Complement C3a Antibody (K13/16-5.7) - BSA Free [NBP1-05122] - Staining in human kidney. Strong staining of plasma of nephritis kidney was seen. Dilution: 1:75.](http://images.novusbio.com/fullsize/Complement-C3a-Antibody-K13-16-5-7-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-05122-img0011.jpg "Immunohistochemistry: Complement C3a Antibody (K13/16-5.7) - BSA Free [NBP1-05122] - Staining in human kidney. Strong staining of plasma of nephritis kidney was seen. Dilution: 1:75.")
![Western Blot: Complement C3a Antibody (K13/16-5.7) - BSA Free [NBP1-05122] - Lane 1: purified C3, lane 2: purifed C3 reduced/DTT, lane 3: purified C3a , lane 4: EDTA-plasma. Lane 5: zymosan-activated serum](http://images.novusbio.com/fullsize/Complement-C3a-Antibody-K13-16-5-7-BSA-Free-Western-Blot-NBP1-05122-img0004.jpg)
![Immunohistochemistry-Paraffin: Complement C3a Antibody (K13/16-5.7) - BSA Free [NBP1-05122] - Staining of human kidney. Strong staining of veins of nephritis kidney was seen. Dilution: 1:150.](http://images.novusbio.com/fullsize/Complement-C3a-Antibody-K13-16-5-7-BSA-Free-Immunohistochemistry-Paraffin-NBP1-05122-img0007.jpg)
![Sandwich ELISA Capture: Complement C3a Antibody (K13/16-5.7) - BSA Free [NBP1-05122] - The calibration curve of a sandwich assay for Complement C3a using NBP1-05122 as the capture antibody and NBP2-23496 as the biotinylated detection antibody.](http://images.novusbio.com/fullsize/Complement-C3a-Antibody-K13-16-5-7-BSA-Free-Sandwich-ELISA-Capture-NBP1-05122-img0005.jpg)
![Western Blot: RHAMM/CD168 Antibody (PSH02-16) [NBP3-32129] - Western blot analysis of RHAMM/CD168 on different lysates with Rabbit anti-RHAMM/CD168 antibody (NBP3-32129) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: SW480 cell lysate Lane 3: HepG2 cell lysate Lane 4: Raji cell lysate Lane 5: MCF7 cell lysate Lane 6: SK-Br-3 cell lysate Lane 7: HeLa cell lysate Lane 8: Jurkat cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 84 kDa Observed band size: 84 kDa Exposure time: 1 minute 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32129) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32129_rabbit-rhamm-cd168-mab-psh02-16-278202415212458.jpg "Western Blot: RHAMM/CD168 Antibody (PSH02-16) [NBP3-32129] - Western blot analysis of RHAMM/CD168 on different lysates with Rabbit anti-RHAMM/CD168 antibody (NBP3-32129) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: SW480 cell lysate Lane 3: HepG2 cell lysate Lane 4: Raji cell lysate Lane 5: MCF7 cell lysate Lane 6: SK-Br-3 cell lysate Lane 7: HeLa cell lysate Lane 8: Jurkat cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 84 kDa Observed band size: 84 kDa Exposure time: 1 minute 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32129) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: RHAMM/CD168 Antibody (PSH02-16) [NBP3-32129] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-RHAMM/CD168 antibody (NBP3-32129) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32129) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32129_rabbit-rhamm-cd168-mab-psh02-16-29820241411547.jpg)
![Immunocytochemistry/ Immunofluorescence: RHAMM/CD168 Antibody (PSH02-16) [NBP3-32129] - Immunocytochemistry analysis of HeLa cells labeling RHAMM/CD168 with Rabbit anti-RHAMM/CD168 antibody (NBP3-32129) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-RHAMM/CD168 antibody (NBP3-32129) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32129_rabbit-rhamm-cd168-mab-psh02-16-2920241114816.jpg)
![Flow Cytometry: RHAMM/CD168 Antibody (PSH02-16) [NBP3-32129] - Flow cytometric analysis of HeLa cells labeling RHAMM/CD168. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32129, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32129_rabbit-rhamm-cd168-mab-psh02-16-392024884629.jpg)
![Flow Cytometry: Fas Receptor/TNFRSF6/CD95 Antibody (LT95) [NB500-503] - ytometry analysis of Fas (CD95) expression on the surface of various hematopoietic cell lines with purified anti-human CD95 (LT95) (detection by Goat anti-mouse IgG1 PE). Histograms: Red - Isotype mouse IgG1 control staining Black - standard anti-CD95 monoclonal antibody (DX2) Green - anti-CD95 (LT95) A - JURKAT human peripheral blood T cell leukemia cell line B - RAMOS human Burkitt lymphoma cell line C - CEM human leukemia cell line D - MOLT-4 human acute lymphoblastic T cell leukemia cell line](http://images.novusbio.com/fullsize/Fas-Receptor-TNFRSF6-CD95-Antibody-LT95-Flow-Cytometry-NB500-503-img0004.jpg "Flow Cytometry: Fas Receptor/TNFRSF6/CD95 Antibody (LT95) [NB500-503] - ytometry analysis of Fas (CD95) expression on the surface of various hematopoietic cell lines with purified anti-human CD95 (LT95) (detection by Goat anti-mouse IgG1 PE). Histograms: Red - Isotype mouse IgG1 control staining Black - standard anti-CD95 monoclonal antibody (DX2) Green - anti-CD95 (LT95) A - JURKAT human peripheral blood T cell leukemia cell line B - RAMOS human Burkitt lymphoma cell line C - CEM human leukemia cell line D - MOLT-4 human acute lymphoblastic T cell leukemia cell line")
![Flow Cytometry: Fas Receptor/TNFRSF6/CD95 Antibody (LT95) [NB500-503] - Fas/TNFRSF6/CD95 Antibody (LT95) [NB500-503] - Fig. 1. Flow cytometry analysis of Fas (CD95) expression on the surface of various hematopoietic cell lines with purified anti-human CD95 (LT95) (detection by Goat anti-mouse IgG1 PE). Histograms: Red - Isotype mouse IgG1 control staining Black - standard anti-CD95 monoclonal antibody Green - anti-CD95 (LT95) A. JURKAT human peripheral blood T cell leukemia cell line B. RAMOS human Burkitt lymphoma cell line C. CEM human leukemia cell line D. MOLT-4 human acute lymphoblastic T cell leukemia cell line.](http://images.novusbio.com/fullsize/Fas-TNFRSF6-CD95-Antibody-LT95-Flow-Cytometry-NB500-503-img0001.jpg)
![Flow Cytometry: Fas Receptor/TNFRSF6/CD95 Antibody (LT95) [NB500-503] - Fas/TNFRSF6/CD95 Antibody (LT95) [NB500-503] - A surface stain was performed on HeLa with Fas/TNFRSF6/CD95 Antibody (LT95) NB500-503AF647 and a matched isotype control. Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/Fas-TNFRSF6-CD95-Antibody-LT95-Flow-Cytometry-NB500-503-img0003.jpg)
![Flow Cytometry: Fas Receptor/TNFRSF6/CD95 Antibody (LT95) [NB500-503] - Fas/TNFRSF6/CD95 Antibody (LT95) [NB500-503] - Using the Allophycocyanin direct conjugate Surface staining of human peripheral blood cells with anti-CD95 (LT95) APC.](http://images.novusbio.com/fullsize/Fas-TNFRSF6-CD95-Antibody-LT95-Flow-Cytometry-NB500-503-img0002.jpg)
![Western Blot: SOX1 Antibody [NBP2-24466] - Western blot analysis of SOX1 in human brain lysate in the 1) absence and 2) presence of immunizing peptide and 3) mouse brain lysate using NBP2-24466 at 0.5 ug/ml, 0.5 ug/ml and 2 ug/ml respectively.](http://images.novusbio.com/fullsize/SOX1-Antibody-Western-Blot-NBP2-24466-img0002.jpg "Western Blot: SOX1 Antibody [NBP2-24466] - Western blot analysis of SOX1 in human brain lysate in the 1) absence and 2) presence of immunizing peptide and 3) mouse brain lysate using NBP2-24466 at 0.5 ug/ml, 0.5 ug/ml and 2 ug/ml respectively.")
![Western Blot: 14-3-3 tau/theta Antibody [NBP1-31537] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membranes were blotted with 14-3-3 theta antibody and a competitor's antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/14-3-3-tau-theta-Antibody-Western-Blot-NBP1-31537-img0005.jpg "<b>Independent Antibodies Validation. </b>Western Blot: 14-3-3 tau/theta Antibody [NBP1-31537] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membranes were blotted with 14-3-3 theta antibody and a competitor's antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Independent Antibodies Validation. </b>Western Blot: 14-3-3 tau/theta Antibody [NBP1-31537] - Sample (20 ug of whole cell lysate) A: mouse brain 12% SDS PAGE, antibody diluted at 1:30000.](http://images.novusbio.com/fullsize/14-3-3-tau-theta-Antibody-Western-Blot-NBP1-31537-img0003.jpg)
![Western Blot: 14-3-3 tau/theta Antibody [NBP1-31537] - Sample(30 ug whole cell lysate) A: 293T B: A431 C: H1299 D: HeLa S3 E: Hep G2 F: MOLT4 G: Raji 12% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/14-3-3-tau-theta-Antibody-Western-Blot-NBP1-31537-img0004.jpg)
![Western Blot: 14-3-3 tau/theta Antibody [NBP1-31537] - Sample (30 ug of whole cell lysate) A: zebrafish eye B: zebrafish heart 12% SDS PAGE NBP1-31537 diluted at 1:1000](http://images.novusbio.com/fullsize/nbp1-31537_rabbit-polyclonal-14-3-3-tau-theta-antibody-91020242021915.jpg)
![Immunohistochemistry-Paraffin: 14-3-3 tau/theta Antibody [NBP1-31537] - 14-3-3 tau/theta antibody detects 14-3-3 tau/theta protein at cytoplasm in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. 14-3-3 tau/theta antibody (NBP1-31537) diluted at 1:500.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/nbp1-31537_rabbit-polyclonal-14-3-3-tau-theta-antibody-91020242041235.jpg)
![Immunohistochemistry-Paraffin: LRRFIP2 Antibody [NBP2-27199] - analysis of LRRFIP2 in formalin-fixed, paraffin-embedded human colon tissue using this antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/LRRFIP2-Antibody-Immunohistochemistry-Paraffin-NBP2-27199-img0001.jpg "Immunohistochemistry-Paraffin: LRRFIP2 Antibody [NBP2-27199] - analysis of LRRFIP2 in formalin-fixed, paraffin-embedded human colon tissue using this antibody at 5 ug/ml.")
![Western Blot: CLEC9a Antibody (14N8D7) [NBP2-27087] - Analysis of A) HCT-116 and B) MCF-7 lysate using this antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/CLEC9a-Antibody-14N8D7-Western-Blot-NBP2-27087-img0003.jpg "Western Blot: CLEC9a Antibody (14N8D7) [NBP2-27087] - Analysis of A) HCT-116 and B) MCF-7 lysate using this antibody at 1 ug/ml.")
![Immunocytochemistry/Immunofluorescence: Glypican 3 Antibody (SPM595) [NBP2-44485] - ICC/IF analysis of MeOH-fixed HepG2 cells labeling Glypican-3 with followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/Glypican-3-Antibody-SPM595-Immunofluorescence-NBP2-44485-img0004.jpg "Immunocytochemistry/Immunofluorescence: Glypican 3 Antibody (SPM595) [NBP2-44485] - ICC/IF analysis of MeOH-fixed HepG2 cells labeling Glypican-3 with followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)")
![Immunohistochemistry-Paraffin: Glypican 3 Antibody (SPM595) [NBP2-44485] - Human Melanoma stained with Glypican-3 Monoclonal Antibody (SPM595)](http://images.novusbio.com/fullsize/Glypican-3-Antibody-SPM595-Immunohistochemistry-Paraffin-NBP2-44485-img0002.jpg)
![Flow Cytometry: Glypican 3 Antibody (SPM595) [NBP2-44485] - Flow Cytometric Analysis of PFA-fixed HepG2 cells using Glypican 3 Antibody (SPM595) followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Glypican-3-Antibody-SPM595-Flow-Cytometry-NBP2-44485-img0003.jpg)
![Immunohistochemistry-Paraffin: Glypican 3 Antibody (SPM595) [NBP2-44485] - Human Hepatocellular Carcinoma stained with Glypican-3 Monoclonal Antibody (SPM595)](http://images.novusbio.com/fullsize/Glypican-3-Antibody-SPM595-Immunohistochemistry-Paraffin-NBP2-44485-img0001.jpg)
![Immunohistochemistry-Paraffin: Neurogenin-3 Antibody [NBP2-27115] - Analysis of Neurogenin3 in FFPE mouse pancreas tissue using NBP2-27115 at 5 ug/ml.](http://images.novusbio.com/fullsize/Neurogenin-3-Antibody-Immunohistochemistry-Paraffin-NBP2-27115-img0002.jpg "Immunohistochemistry-Paraffin: Neurogenin-3 Antibody [NBP2-27115] - Analysis of Neurogenin3 in FFPE mouse pancreas tissue using NBP2-27115 at 5 ug/ml.")
![Immunoprecipitation: Lamin A + C Antibody (4H7) [NBP3-26167] - Immunoprecipitating Lamin A + C in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26167 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26167 (3ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (20ug).](http://images.novusbio.com/fullsize/nbp3-26167_rabbit-lamin-a-c-mab-4h7-262202413221512.jpg "Immunoprecipitation: Lamin A + C Antibody (4H7) [NBP3-26167] - Immunoprecipitating Lamin A + C in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26167 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26167 (3ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (20ug).")
![Immunohistochemistry: Lamin A + C Antibody (4H7) [NBP3-26167] - Image of Lamin A + C Antibody (4H7) diluted at 1:115 and staining in paraffin-embedded human glioma cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26167_rabbit-lamin-a-c-mab-4h7-262202414471576.jpg)
![Immunohistochemistry: Lamin A + C Antibody (4H7) [NBP3-26167] - Image of Lamin A + C Antibody (4H7) diluted at 1:115 and staining in paraffin-embedded human glioma cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26167_rabbit-lamin-a-c-mab-4h7-2722024820395.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (4H7) [NBP3-26167] - Staining of Hela cells with Lamin A + C Antibody (4H7) at 1:38, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26167_rabbit-lamin-a-c-mab-4h7-282202412493637.jpg)
![Flow Cytometry: Lamin A + C Antibody (4H7) [NBP3-26167] - Overlay histogram showing Hela cells stained with Lamin A + C Antibody (4H7) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26167_rabbit-lamin-a-c-mab-4h7-282202414312758.jpg)