| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-189SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-71691SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84971-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84576-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-13878-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-79816SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87401-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87383-25ul | |||
![Western Blot: VAP-A Antibody [NBP1-31237] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with VAPA antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/VAP-A-Antibody-Western-Blot-NBP1-31237-img0013.jpg "Western Blot: VAP-A Antibody [NBP1-31237] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with VAPA antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: VAP-A Antibody [NBP1-31237] - WDR44 tubules are in close contact with the ER via binding to VAPA/B. Confocal images of transfected HeLa cells showing colocalization of GRAF2-RFP tubules with GFP-VAPA. Image collected and cropped by CiteAb from the following publication (//rupress.org/jcb/article/doi/10.1083/jcb.201811014/151714/GRAF2-WDR44-and-MICAL1-mediate-Rab81011dependent) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/VAP-A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31237-img0014.jpg)
![Immunohistochemistry-Paraffin: VAP-A Antibody [NBP1-31237] - Human breast cancer, using VAPA antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/VAP-A-Antibody-Immunohistochemistry-Paraffin-NBP1-31237-img0007.jpg)
![Western Blot: VAP-A Antibody [NBP1-31237] - Analysis of antibody on A:NIH-3T3 12% SDS PAGE diluted at 1:1000](http://images.novusbio.com/fullsize/VAP-A-Antibody-Western-Blot-NBP1-31237-img0008.jpg)
![Western Blot: VAP-A Antibody [NBP1-31237] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with VAPA antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/VAP-A-Antibody-Western-Blot-NBP1-31237-img0011.jpg)
![Immunohistochemistry-Paraffin: VAP-A Antibody [NBP1-31237] - Human lung adenocarcinoma, using VAPA antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/VAP-A-Antibody-Immunohistochemistry-Paraffin-NBP1-31237-img0006.jpg)
![Western Blot: VAP-A Antibody [NBP1-31237] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with VAP-A antibody [N1N3] (NBP1-31237) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31237_rabbit-polyclonal-vap-a-antibody-91020242001081.jpg)
![Western Blot: metabotropic Glutamate Receptor 6 Antibody [NB300-189] - Detection of metabotropic Glutamate Receptor 6 in mouse brain lysate at 0.5 ug/ml.](http://images.novusbio.com/fullsize/metabotropic-Glutamate-Receptor-6-Antibody-Western-Blot-NB300-189-img0006.jpg "Western Blot: metabotropic Glutamate Receptor 6 Antibody [NB300-189] - Detection of metabotropic Glutamate Receptor 6 in mouse brain lysate at 0.5 ug/ml.")
![Immunocytochemistry/Immunofluorescence: mGluR6 Antibody [NB300-189] - metabotropic Glutamate Receptor 6 Antibody [NB300-189] - Detection of metabotropic Glutamate Receptor 6 in trapezoid body in mouse brain using NB300-189 and anti-rabbit Cy3 conjugated secondary antibodies (red). DAPI was used to counterstain cell nuclei.](http://images.novusbio.com/fullsize/metabotropic-Glutamate-Receptor-6-Antibody-Immunocytochemistry-Immunofluorescence-NB300-189-img0005.jpg)
![Western Blot: NUP62 Antibody [NBP1-31381] - Whole cell extracts (30 ug) was separated by 10% SDS-PAGE, and blotted with Nucleoporin p62 antibody diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/NUP62-Antibody-Western-Blot-NBP1-31381-img0015.jpg "Western Blot: NUP62 Antibody [NBP1-31381] - Whole cell extracts (30 ug) was separated by 10% SDS-PAGE, and blotted with Nucleoporin p62 antibody diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Nucleoporin p62 Antibody [NBP1-31381] - Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Nucleoporin p62 protein stained by Nucleoporin p62 antibody diluted at 1:750. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/Nucleoporin-p62-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31381-img0012.jpg)
![Immunohistochemistry-Paraffin: Nucleoporin p62 Antibody [NBP1-31381] - Paraffin-embedded NCIN87 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Nucleoporin-p62-Antibody-Immunohistochemistry-Paraffin-NBP1-31381-img0008.jpg)
![Western Blot: Nucleoporin p62 Antibody [NBP1-31381] - PC-12 whole cell lysate (30 ug) stained wtih Nucleoporin p62 Antibody diluted at 1:5000.](http://images.novusbio.com/fullsize/Nucleoporin-p62-Antibody-Western-Blot-NBP1-31381-img0002.jpg)
![Western Blot: Nucleoporin p62 Antibody [NBP1-31381] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 D: C2C12 7. 5% SDS PAGE; antibody diluted at 1:5000.](http://images.novusbio.com/fullsize/Nucleoporin-p62-Antibody-Western-Blot-NBP1-31381-img0009.jpg)
![Immunoprecipitation: Nucleoporin p62 Antibody [NBP1-31381] - A. Control with 4 ug of preimmune Rabbit IgGB. Immunoprecipitation of NUP62 protein by 4 ug Nucleoporin p62 antibody 7.5 % SDS-PAGE The immunoprecipitated NUP62 protein was detected by Nucleoporin p62 antibody diluted at 1:1000.[EasyBlot anti-rabbit IgG) was used as a secondary reagent]](http://images.novusbio.com/fullsize/Nucleoporin-p62-Antibody-Immunoprecipitation-NBP1-31381-img0010.jpg)
![Immunohistochemistry-Paraffin: NUP62 Antibody [NBP1-31381] - NUP62 antibody detects NUP62 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.NUP62 stained by NUP62 antibody (NBP1-31381) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31381_rabbit-polyclonal-nup62-antibody-91020242014638.jpg)
![Western Blot: Peroxiredoxin 6 Antibody (3A10-2A11) [H00009588-M01] - PRDX6 monoclonal antibody (M01), clone S1. Analysis of PRDX6 expression in MCF-7.](http://images.novusbio.com/fullsize/Peroxiredoxin-6-Antibody-3A10-2A11-Western-Blot-H00009588-M01-img0002.jpg "Western Blot: Peroxiredoxin 6 Antibody (3A10-2A11) [H00009588-M01] - PRDX6 monoclonal antibody (M01), clone S1. Analysis of PRDX6 expression in MCF-7.")
![Western Blot: Peroxiredoxin 6 Antibody (3A10-2A11) [H00009588-M01] - PRDX6 monoclonal antibody (M01), clone 3A10-2A11. Analysis of PRDX6 expression in human liver.](http://images.novusbio.com/fullsize/Peroxiredoxin-6-Antibody-3A10-2A11-Western-Blot-H00009588-M01-img0003.jpg)
![ELISA: Peroxiredoxin 6 Antibody (3A10-2A11) [H00009588-M01] - Detection limit for recombinant GST tagged PRDX6 is 3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Peroxiredoxin-6-Antibody-3A10-2A11-Sandwich-ELISA-H00009588-M01-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Dicer Antibody [NBP1-71691] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Dicer at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Dicer-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-71691-img0008.jpg "Immunocytochemistry/Immunofluorescence: Dicer Antibody [NBP1-71691] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Dicer at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Immunohistochemistry-Paraffin: Dicer Antibody [NBP1-71691] - Analysis of Dicer in paraffin embedded human colorectal cancer tissue using anti-Dicer antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Dicer-Antibody-Immunohistochemistry-Paraffin-NBP1-71691-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Dicer Antibody [NBP1-71691] - Dicer antibody was tested in HeLa cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Dicer-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-71691-img0005.jpg)
![Immunohistochemistry: Dicer Antibody [NBP1-71691] - Staining of Dicer in mouse prostate using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Dicer-Antibody-Immunohistochemistry-NBP1-71691-img0006.jpg)
![Immunohistochemistry-Paraffin: Biglycan Antibody [NBP1-84971] - Analysis in human placenta and skeletal muscle tissues. Corresponding BGN RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Biglycan-Antibody-Immunohistochemistry-Paraffin-NBP1-84971-img0018.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Biglycan Antibody [NBP1-84971] - Analysis in human placenta and skeletal muscle tissues. Corresponding BGN RNA-seq data are presented for the same tissues.")
![<b>Biological Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: Biglycan Antibody [NBP1-84971] - Treatment of CAFs with combined cytokines or cancer cell-derived secretomes affects ECM protein expression. Western blot showing the effects of bFGF, EGF and TGF beta 1 treatment (10 ng/ml) on the expression of biglycan in CAFs. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25593993/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Biglycan-Antibody-Western-Blot-NBP1-84971-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: Biglycan Antibody [NBP1-84971] - Staining of human cell line BJ shows localization to endoplasmic reticulum & the Golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Biglycan-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84971-img0006.jpg)
![Western Blot: Biglycan Antibody [NBP1-84971] - Analysis in human cell line U-138 MG.](http://images.novusbio.com/fullsize/Biglycan-Antibody-Western-Blot-NBP1-84971-img0005.jpg)
![Western Blot: Biglycan Antibody [NBP1-84971] - Analysis in control (vector only transfected HEK293T lysate) and BGN over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/Biglycan-Antibody-Western-Blot-NBP1-84971-img0013.jpg)
![Immunohistochemistry-Paraffin: Biglycan Antibody [NBP1-84971] - Staining of human testis shows weak to moderate cytoplasmic positivity in peritubular myoid cells.](http://images.novusbio.com/fullsize/Biglycan-Antibody-Immunohistochemistry-Paraffin-NBP1-84971-img0014.jpg)
![Immunohistochemistry-Paraffin: Biglycan Antibody [NBP1-84971] - Staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Biglycan-Antibody-Immunohistochemistry-Paraffin-NBP1-84971-img0016.jpg)
![Immunohistochemistry-Paraffin: Biglycan Antibody [NBP1-84971] - Staining of human kidney shows moderate extracellular space positivity.](http://images.novusbio.com/fullsize/Biglycan-Antibody-Immunohistochemistry-Paraffin-NBP1-84971-img0015.jpg)
![Immunohistochemistry-Paraffin: Biglycan Antibody [NBP1-84971] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Biglycan-Antibody-Immunohistochemistry-Paraffin-NBP1-84971-img0017.jpg)
![Western Blot: Biglycan Antibody [NBP1-84971] - Treatment of CAFs with combined cytokines or cancer cell-derived secretomes affects ECM protein expression(A) Western blot showing the effects of bFGF, EGF & TGF-beta 1 treatment (10 ng/ml) on the expression of biglycan, decorin, alpha -SMA & versican in CAFs. (B) Western blot illustrating the effects of treatment with cancer cell-derived conditioned medium (CM) on the expression of biglycan, decorin, alpha -SMA & versican in CAFs. TGF-beta 1 treatment (1 & 10 ng/ml) was included as a positive control. Tubulin was used as loading control (A, B). Image collected & cropped by CiteAb from the following publication (//www.oncoscience.us/lookup/doi/10.18632/oncoscience.87), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-84971_rabbit-polyclonal-biglycan-antibody-310202415394533.jpg)
![Western Blot: Biglycan Antibody [NBP1-84971] - Treatment of CAFs with combined cytokines or cancer cell-derived secretomes affects ECM protein expression(A) Western blot showing the effects of bFGF, EGF & TGF-beta 1 treatment (10 ng/ml) on the expression of biglycan, decorin, alpha -SMA & versican in CAFs. (B) Western blot illustrating the effects of treatment with cancer cell-derived conditioned medium (CM) on the expression of biglycan, decorin, alpha -SMA & versican in CAFs. TGF-beta 1 treatment (1 & 10 ng/ml) was included as a positive control. Tubulin was used as loading control (A, B). Image collected & cropped by CiteAb from the following publication (//www.oncoscience.us/lookup/doi/10.18632/oncoscience.87), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-84971_rabbit-polyclonal-biglycan-antibody-310202415392538.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody [NBP1-84576] - Staining in human placenta and skeletal muscle tissues using anti-ITGA5 antibody. Corresponding ITGA5 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-Immunohistochemistry-Paraffin-NBP1-84576-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody [NBP1-84576] - Staining in human placenta and skeletal muscle tissues using anti-ITGA5 antibody. Corresponding ITGA5 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody [NBP1-84576] - Staining of human endometrium shows weak to moderate membranous positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-Immunohistochemistry-Paraffin-NBP1-84576-img0014.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody [NBP1-84576] - Staining of human placenta shows high expression.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-Immunohistochemistry-Paraffin-NBP1-84576-img0010.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody [NBP1-84576] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-Immunohistochemistry-Paraffin-NBP1-84576-img0011.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody [NBP1-84576] - Staining of human prostate shows moderate membranous positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-Immunohistochemistry-Paraffin-NBP1-84576-img0013.jpg)
![Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody [NBP2-13878] - Staining of human cerebral cortex, heart muscle, kidney and pancreas using Anti-CS antibody NBP2-13878 (A) shows similar protein distribution across tissues to independent antibody NBP2-13877 (B).](http://images.novusbio.com/fullsize/CS-Citrate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP2-13878-img0011.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody [NBP2-13878] - Staining of human cerebral cortex, heart muscle, kidney and pancreas using Anti-CS antibody NBP2-13878 (A) shows similar protein distribution across tissues to independent antibody NBP2-13877 (B).")
![<b>Independent Antibodies Validation. </b>Western Blot: CS Citrate Synthase Antibody [NBP2-13878] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/CS-Citrate-Synthase-Antibody-Western-Blot-NBP2-13878-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: CS Citrate Synthase Antibody [NBP2-13878] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CS-Citrate-Synthase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13878-img0007.jpg)
![Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody [NBP2-13878] - Staining of human cererbellum shows moderate to strong cytoplasmic positivity in purkinje cells.](http://images.novusbio.com/fullsize/CS-Citrate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP2-13878-img0017.jpg)
![Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody [NBP2-13878] - Staining of human heart muscle shows high expression.](http://images.novusbio.com/fullsize/CS-Citrate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP2-13878-img0008.jpg)
![Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody [NBP2-13878] - Staining of human kidney.](http://images.novusbio.com/fullsize/CS-Citrate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP2-13878-img0012.jpg)
![Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody [NBP2-13878] - Staining of human skeletal muscle shows weak to moderate cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/CS-Citrate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP2-13878-img0013.jpg)
![Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody [NBP2-13878] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/CS-Citrate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP2-13878-img0014.jpg)
![Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody [NBP2-13878] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/CS-Citrate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP2-13878-img0016.jpg)
![Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody [NBP2-13878] - Staining of human pancreas.](http://images.novusbio.com/fullsize/CS-Citrate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP2-13878-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: FLNC Antibody - BSA Free [NBP2-79816] - U2OS cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with FLNC Antibody (NBP2-79816) at 1 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/FLNC-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-79816-img0007.jpg "Immunocytochemistry/Immunofluorescence: FLNC Antibody - BSA Free [NBP2-79816] - U2OS cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with FLNC Antibody (NBP2-79816) at 1 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Immunocytochemistry/Immunofluorescence: FLNC Antibody - BSA Free [NBP2-79816] - U2OS cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with FLNC Antibody conjugated to Alexa Fluor 647 (NBP2-79816AF647) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/FLNC-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-79816-img0006.jpg)
![Western Blot: FLNC Antibody [NBP2-79816] - Total protein from HeLa was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-FLNC in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/FLNC-Antibody-Western-Blot-NBP2-79816-img0004.jpg)
![Immunohistochemistry-Paraffin: FLNC Antibody [NBP2-79816] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of human skeletal muscle using FLNC antibody (NBP2-79816) at 1:200 dilution. The primary antibody binding to FLNC was detected using HRP conjugated anti-rabbit secondary antibody with DAB reagent, and the sections were further counterstained with hematoxylin for labeling cellular nuclei. Strong staining in the cytoplasm of myocytes was observed.](http://images.novusbio.com/fullsize/FLNC-Antibody-Immunohistochemistry-Paraffin-NBP2-79816-img0002.jpg)
![Flow Cytometry: FLNC Antibody - BSA Free [NBP2-79816] - An intracellular stain was performed on U2-OS cells with FLNC NBP2-79816AF647 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/FLNC-Antibody---BSA-Free-Flow-Cytometry-NBP2-79816-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: FLNC Antibody [NBP2-79816] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-FLNC at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/FLNC-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-79816-img0001.jpg)
![Flow Cytometry: FLNC Antibody [NBP2-79816] - An intracellular stain was performed on NIH3T3 cells with NBP2-79816 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.](http://images.novusbio.com/fullsize/FLNC-Antibody-Flow-Cytometry-NBP2-79816-img0005.jpg)
![Immunohistochemistry-Paraffin: Adenylate Kinase 1 Antibody [NBP1-87401] - Staining in human fallopian tube and small intestine tissues using anti-AK1 antibody. Corresponding AK1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Adenylate-Kinase-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87401-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Adenylate Kinase 1 Antibody [NBP1-87401] - Staining in human fallopian tube and small intestine tissues using anti-AK1 antibody. Corresponding AK1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Adenylate Kinase 1 Antibody [NBP1-87401] - Lane 1: Marker [kDa] 229, 112, 84, 48, 32, 27, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/Adenylate-Kinase-1-Antibody-Western-Blot-NBP1-87401-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Adenylate Kinase 1 Antibody [NBP1-87401] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Adenylate-Kinase-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87401-img0006.jpg)
![Immunohistochemistry-Paraffin: Adenylate Kinase 1 Antibody [NBP1-87401] - Staining of human small intestine shows low expression as expected.](http://images.novusbio.com/fullsize/Adenylate-Kinase-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87401-img0008.jpg)
![Western Blot: Adenylate Kinase 1 Antibody [NBP1-87401] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/Adenylate-Kinase-1-Antibody-Western-Blot-NBP1-87401-img0004.jpg)
![Immunohistochemistry-Paraffin: Adenylate Kinase 1 Antibody [NBP1-87401] - Staining of human fallopian tube shows high expression.](http://images.novusbio.com/fullsize/Adenylate-Kinase-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87401-img0007.jpg)
![Western Blot: Nesfatin-1/Nucleobindin-2 Antibody [NBP1-87383] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Nesfatin-1-Nucleobindin-2-Antibody-Western-Blot-NBP1-87383-img0010.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Nesfatin-1/Nucleobindin-2 Antibody [NBP1-87383] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Nesfatin-1/Nucleobindin-2 Antibody [NBP1-87383] - Staining of human cell line U-2 OS shows localization to the Golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Nesfatin-1-Nucleobindin-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87383-img0009.jpg)
![Immunohistochemistry-Paraffin: Nesfatin-1/Nucleobindin-2 Antibody [NBP1-87383] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Nesfatin-1-Nucleobindin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-87383-img0002.jpg)
![Western Blot: Nesfatin-1/Nucleobindin-2 Antibody [NBP1-87383] - Analysis in human cell line HL-60.](http://images.novusbio.com/fullsize/Nesfatin-1-Nucleobindin-2-Antibody-Western-Blot-NBP1-87383-img0007.jpg)
![Immunohistochemistry-Paraffin: Nesfatin-1/Nucleobindin-2 Antibody [NBP1-87383] -Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/antibody/nbp1-87383_rabbit-polyclonal-nesfatin-1-nucleobindin-2-antibody-immunohistochemistry-paraffin-25520239927..jpg)
![Immunohistochemistry-Paraffin: Nesfatin-1/Nucleobindin-2 Antibody [NBP1-87383] -Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/antibody/nbp1-87383_rabbit-polyclonal-nesfatin-1-nucleobindin-2-antibody-immunohistochemistry-paraffin-255202391111..jpg)
![Immunohistochemistry-Paraffin: Nesfatin-1/Nucleobindin-2 Antibody [NBP1-87383] -Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/antibody/nbp1-87383_rabbit-polyclonal-nesfatin-1-nucleobindin-2-antibody-immunohistochemistry-paraffin-255202391223..jpg)
![Western Blot: RhoA Antibody (1A11-4G10) [NBP2-22528] - Analysis of 25 ug of various whole cell lysates or 0.5 ug of purified protein (full-length His-RhoA, truncated GST-RhoB or full-length GST-RhoC fusion) per well onto a 4-20% Tris-HCl polyacrylamide gel.](http://images.novusbio.com/fullsize/RhoA-Antibody-1A11-4G10-Western-Blot-NBP2-22528-img0001.jpg "Western Blot: RhoA Antibody (1A11-4G10) [NBP2-22528] - Analysis of 25 ug of various whole cell lysates or 0.5 ug of purified protein (full-length His-RhoA, truncated GST-RhoB or full-length GST-RhoC fusion) per well onto a 4-20% Tris-HCl polyacrylamide gel.")
![Immunocytochemistry/Immunofluorescence: RhoA Antibody (1A11-4G10) [NBP2-22528] - Analysis of Rho A/B/C (green) showing staining in the in the cytoplasm of A431 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RhoA-Antibody-1A11-4G10-Immunocytochemistry-Immunofluorescence-NBP2-22528-img0005.jpg)
![Immunohistochemistry-Paraffin: RhoA Antibody (1A11-4G10) [NBP2-22528] - Analysis showing staining in the cytoplasm of human hepatocarcinoma (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RhoA-Antibody-1A11-4G10-Immunohistochemistry-Paraffin-NBP2-22528-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: RhoA Antibody (1A11-4G10) [NBP2-22528] - Analysis of RhoA/B/C (green) in NIH3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% BSA/TBST for 15 minutes at room temperature. Cells were probed with a RhoA/B/C monoclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488-conjugated goat-anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/RhoA-Antibody-1A11-4G10-Immunocytochemistry-Immunofluorescence-NBP2-22528-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: RhoA Antibody (1A11-4G10) [NBP2-22528] - Analysis of Rho A/B/C (green) showing staining in the in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RhoA-Antibody-1A11-4G10-Immunocytochemistry-Immunofluorescence-NBP2-22528-img0004.jpg)
![Immunohistochemistry-Paraffin: RhoA Antibody (1A11-4G10) [NBP2-22528] - Analysis of showing staining in the cytoplasm of mouse breast tissue (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RhoA-Antibody-1A11-4G10-Immunohistochemistry-Paraffin-NBP2-22528-img0006.jpg)
![Immunohistochemistry-Paraffin: RhoA Antibody (1A11-4G10) [NBP2-22528] - Analysis showing staining in the cytoplasm of human prostate carcinoma (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RhoA-Antibody-1A11-4G10-Immunohistochemistry-Paraffin-NBP2-22528-img0007.jpg)
![Immunoprecipitation: RhoA Antibody (1A11-4G10) [NBP2-22528] - Analysis of RhoA/B/C was performed on HeLa cells. Antigen-antibody complexes were formed by incubating 750 ug of HeLa cell lysate with 2 ug of a RhoA/B/C monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50 ul Protein A/G Plus Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer. Eluted sample (right lane) and 25 ug of HeLa cell lysate (left lane) were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a Rho A/B/C monoclonal antibody at a dilution of 1:1000 overnight rotating at 4C, washed in TBST, and probed with Clean-blot IP Detection Reagent at a dilution of 1:2500 for at least 1 hour.](http://images.novusbio.com/fullsize/RhoA-Antibody-1A11-4G10-Immunoprecipitation-NBP2-22528-img0002.jpg)
