| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56649SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2519SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84123-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85550-25ul | |||
![Western Blot: Lamin A + C Antibody [NBP2-19324] - Wild-type (WT) and Lamin A + C knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody. The HRP-conjugated anti-rabbit IgG (NBP2-19301) antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Western-Blot-NBP2-19324-img0017.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Lamin A + C Antibody [NBP2-19324] - Wild-type (WT) and Lamin A + C knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody. The HRP-conjugated anti-rabbit IgG (NBP2-19301) antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody [NBP2-19324] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Lamin A + C stained by Lamin A + C antibody diluted at 1:1000. Red: alpha Tubulin, stained by alpha Tubulin antibody [114] (NBP2-43837) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19324-img0020.jpg)
![Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19324] - Mouse stomach. Lamin A + C stained by Lamin A + C antibody diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunohistochemistry-Paraffin-NBP2-19324-img0022.jpg)
![Western Blot: Lamin A + C Antibody [NBP2-19324] - Sample (30 ug of whole cell lysate) A: NIH-3T3 7.5% SDS PAGE diluted at 1:1000.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Western-Blot-NBP2-19324-img0009.jpg)
![Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19324] - C2C12 xenograft, using Lamin A + C antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunohistochemistry-Paraffin-NBP2-19324-img0003.jpg)
![Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19324] - RT2 xenograft, using Lamin A + C antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunohistochemistry-Paraffin-NBP2-19324-img0005.jpg)
![Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19324] - Human breast carcinoma. Lamin A + C antibody diluted at 1:250. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunohistochemistry-Paraffin-NBP2-19324-img0007.jpg)
![Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19324] - Mahlarvu xenograft, using Lamin A + C antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunohistochemistry-Paraffin-NBP2-19324-img0018.jpg)
![Immunoprecipitation: Lamin A + C Antibody [NBP2-19324] - A. 50 ug HeLa whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of Lamin A + C protein by 4 ug Lamin A + C antibody (NBP2-19324)7.5 % SDS-PAGE](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunoprecipitation-NBP2-19324-img0015.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Lamin A + C Antibody [NBP2-19324] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-19324_rabbit-polyclonal-lamin-a-c-antibody-1610202419414527.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Lamin A + C Antibody [NBP2-19324] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-19324_rabbit-polyclonal-lamin-a-c-antibody-1610202419405760.jpg)
![Immunohistochemistry: SIRP alpha/CD172a Antibody (OX-41) [NB100-65530] - Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a as a detection reagent. High power](http://images.novusbio.com/fullsize/SIRP-alpha-CD172a-Antibody-OX-41-Immunohistochemistry-NB100-65530-img0007.jpg "Immunohistochemistry: SIRP alpha/CD172a Antibody (OX-41) [NB100-65530] - Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a as a detection reagent. High power")
![Immunohistochemistry: SIRP alpha/CD172a Antibody (OX-41) [NB100-65530] - Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a as a detection reagent. Low power](http://images.novusbio.com/fullsize/SIRP-alpha-CD172a-Antibody-OX-41-Immunohistochemistry-NB100-65530-img0004.jpg)
![Immunohistochemistry: SIRP alpha/CD172a Antibody (OX-41) [NB100-65530] - Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a as a detection reagent. Medium powerPrevious](http://images.novusbio.com/fullsize/SIRP-alpha-CD172a-Antibody-OX-41-Immunohistochemistry-NB100-65530-img0005.jpg)
![Immunohistochemistry: SIRP alpha/CD172a Antibody (OX-41) [NB100-65530] - Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a as a detection reagent. High power](http://images.novusbio.com/fullsize/SIRP-alpha-CD172a-Antibody-OX-41-Immunohistochemistry-NB100-65530-img0006.jpg)
![Immunofluorescence: SIRP alpha/CD172a Antibody (OX-41) [NB100-65530] - Staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody, red in A and Mouse anti Rat CD4, green in B. C is the Merged image with nuclei counter-stained blue using DAPI. Low power](http://images.novusbio.com/fullsize/SIRP-alpha-CD172a-Antibody-OX-41-Immunofluorescence-NB100-65530-img0001.jpg)
![Immunofluorescence: SIRP alpha/CD172a Antibody (OX-41) [NB100-65530] - Staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody, red in A and Mouse anti Rat CD4, green in B. C is the Merged image with nuclei counter-stained blue using DAPI. Medium power](http://images.novusbio.com/fullsize/SIRP-alpha-CD172a-Antibody-OX-41-Immunofluorescence-NB100-65530-img0002.jpg)
![Immunofluorescence: SIRP alpha/CD172a Antibody (OX-41) [NB100-65530] - Staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody, red in A and Mouse anti Rat CD4, green in B. C is the Merged image with nuclei counter-stained blue using DAPI. High power](http://images.novusbio.com/fullsize/SIRP-alpha-CD172a-Antibody-OX-41-Immunofluorescence-NB100-65530-img0003.jpg)
![Western Blot: Lamin A + C Antibody [NB100-56649] - Analysis of human Lamin A + C in 15 ug of HeLa cell lysate using NB100-56649 at 1:1000.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Western-Blot-NB100-56649-img0003.jpg "Western Blot: Lamin A + C Antibody [NB100-56649] - Analysis of human Lamin A + C in 15 ug of HeLa cell lysate using NB100-56649 at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody [NB100-56649] - Lamin A/C antibody was tested in A431 cells at 1:100 against DyLight 488 (Green). Alpha tubulin and nuclei were counterstained against DyLight 568 (Red) and DAPI (Blue).](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56649-img0005.jpg)
![Flow (Intracellular): Lamin A + C Antibody [NB100-56649] - Intracellular staining of HeLa cells (1 x 10^6 cells/mL) with Lamin A+C antibody (orange) stained at 1:1000. Detected with a GtxRb DyLight 488 secondary. Shown with the secondary only control (blue).](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Flow-Intracellular-NB100-56649-img0004.jpg)
![Western Blot: Laforin/EPM2A Antibody (6C6) [H00007957-M02] - EPM2A monoclonal antibody (M02), clone 6C6 Analysis of EPM2A expression in PC-12.](http://images.novusbio.com/fullsize/Laforin-EPM2A-Antibody-6C6-Western-Blot-H00007957-M02-img0005.jpg "Western Blot: Laforin/EPM2A Antibody (6C6) [H00007957-M02] - EPM2A monoclonal antibody (M02), clone 6C6 Analysis of EPM2A expression in PC-12.")
![Western Blot: Laforin/EPM2A Antibody (6C6) [H00007957-M02] - Analysis of EPM2A expression in transfected 293T cell line by EPM2A monoclonal antibody (M02), clone 6C6.Lane 1: EPM2A transfected lysate(37.2 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Laforin-EPM2A-Antibody-6C6-Western-Blot-H00007957-M02-img0006.jpg)
![Sandwich ELISA: Laforin/EPM2A Antibody (6C6) [H00007957-M02] - Detection limit for recombinant GST tagged EPM2A is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Laforin-EPM2A-Antibody-6C6-Sandwich-ELISA-H00007957-M02-img0004.jpg)
![Western Blot: Lamin A + C Antibody [NBP2-25153] - Stripe blot of crude HeLa cell extract. Note two strong and clean bands at 74kDa and 65kDa, corresponding to Lamin A and C.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Western-Blot-NBP2-25153-img0001.jpg "Western Blot: Lamin A + C Antibody [NBP2-25153] - Stripe blot of crude HeLa cell extract. Note two strong and clean bands at 74kDa and 65kDa, corresponding to Lamin A and C.")
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody [NBP2-25153] - HeLa cells stained with NBP2-25153 (red) counterstained with monoclonal antibody to Hsp27, NBP2-25149 (green) and DNA (blue). NBP2-25153 reveals strong nuclear lamina staining, while NBP2-25149 reveals strong cytoplasmic staining. Since both DNA (blue) and Lamin A + C (red) are associated with the nuclear compartment, this region appears crimson in this image.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-25153-img0003.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody [NB100-2519] - Ethanol induces the expression of heat shock proteins (HSPs) in primary astrocyte culture. Increase in alphabeta-crystallin protein levels after treatment for 2 h with 60 mmol/L ethanol (EtOH) or 42C heat shock (HS) in primary astrocyte culture. alpha-tubulin used as an internal standard. The bar graph to the right of the immunoblots represents the quantification of immunoreactive bands intensities normalized to the internal standard, expressed in arbitrary optical density (OD) units. The data are the mean +/- SEM of normalized relative OD values analyzed by one-way ANOVA with Dunnett's multiple comparison post hoc test, n = 3 (significantly differently at the level of *P < 0.05, **P < 0.01, ***P < 0.001). Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1002/brb3.125) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Western-Blot-NB100-2519-img0007.jpg "<b>Biological Strategies Validation. </b>Western Blot: AlphaB Crystallin/CRYAB Antibody [NB100-2519] - Ethanol induces the expression of heat shock proteins (HSPs) in primary astrocyte culture. Increase in alphabeta-crystallin protein levels after treatment for 2 h with 60 mmol/L ethanol (EtOH) or 42C heat shock (HS) in primary astrocyte culture. alpha-tubulin used as an internal standard. The bar graph to the right of the immunoblots represents the quantification of immunoreactive bands intensities normalized to the internal standard, expressed in arbitrary optical density (OD) units. The data are the mean +/- SEM of normalized relative OD values analyzed by one-way ANOVA with Dunnett's multiple comparison post hoc test, n = 3 (significantly differently at the level of *P < 0.05, **P < 0.01, ***P < 0.001). Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1002/brb3.125) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody [NB100-2519] - CRYAB antibody was tested in SHSH5Y cells with DyLight488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2519-img0004.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody [NB100-2519] - Detection of CryAB using NB 100-2519 (0.5ug/ml). Lane 1: Human skeletal muscle Lane 2: Mouse skeletal muscle.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Western-Blot-NB100-2519-img0005.jpg)
![Immunohistochemistry-Paraffin: ATAD2 Antibody [NBP1-84123] - Analysis in human testis and skeletal muscle tissues using NBP1-84123 antibody. Corresponding ATAD2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ATAD2-Antibody-Immunohistochemistry-Paraffin-NBP1-84123-img0014.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ATAD2 Antibody [NBP1-84123] - Analysis in human testis and skeletal muscle tissues using NBP1-84123 antibody. Corresponding ATAD2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ATAD2 Antibody [NBP1-84123] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ATAD2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84123-img0012.jpg)
![Immunohistochemistry-Paraffin: ATAD2 Antibody [NBP1-84123] - Staining of human Skeletal muscle shows very weak positivity in myocytes.](http://images.novusbio.com/fullsize/ATAD2-Antibody-Immunohistochemistry-Paraffin-NBP1-84123-img0017.jpg)
![Immunohistochemistry-Paraffin: ATAD2 Antibody [NBP1-84123] - Staining of human Bone marrow shows strong nuclear positivity in hematopoietic cells.](http://images.novusbio.com/fullsize/ATAD2-Antibody-Immunohistochemistry-Paraffin-NBP1-84123-img0015.jpg)
![Immunohistochemistry-Paraffin: ATAD2 Antibody [NBP1-84123] - Staining of human Lymph node shows moderate nuclear positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/ATAD2-Antibody-Immunohistochemistry-Paraffin-NBP1-84123-img0016.jpg)
![Immunohistochemistry-Paraffin: ATAD2 Antibody [NBP1-84123] - Staining of human Testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/ATAD2-Antibody-Immunohistochemistry-Paraffin-NBP1-84123-img0018.jpg)
![Simple Western: ATAD2 Antibody [NBP1-84123] - Simple Western lane view shows a specific band for ATAD2 in 0.2 mg/ml of U-251MG sp lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system.](http://images.novusbio.com/fullsize/ATAD2-Antibody-Simple-Western-NBP1-84123-img0006.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454] - Western blot analysis of extracts (35ug) from 9 different cell lines by using AlphaB Crystallin/CRYAB Antibody (OTI6A9). (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Azide-and-BSA-Free-Western-Blot-NBP2-70454-img0002.jpg "Western Blot: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454] - Western blot analysis of extracts (35ug) from 9 different cell lines by using AlphaB Crystallin/CRYAB Antibody (OTI6A9). (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454] - Immunofluorescent staining of HT29 cells using AlphaB Crystallin/CRYAB Antibody (OTI6A9) (NBP2-70454).](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70454-img0006.jpg)
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454] - Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using AlphaB Crystallin/CRYAB Antibody (OTI6A9). (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min, 0454)](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-70454-img0004.jpg)
![Flow Cytometry: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454] - HEK293T cells transfected with either RC202718 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by AlphaB Crystallin/CRYAB Antibody (OTI6A9) (NBP2-70454), and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70454-img0008.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRYAB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with AlphaB Crystallin/CRYAB Antibody (OTI6A9).](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Azide-and-BSA-Free-Western-Blot-NBP2-70454-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454] - AlphaB Crystallin/CRYAB Antibody (OTI6A9) (NBP2-70454) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CRYAB.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70454-img0005.jpg)
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454] - Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using AlphaB Crystallin/CRYAB Antibody (OTI6A9). (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min, NBP2-70454)](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-70454-img0003.jpg)
![Immunoprecipitation: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454] - Immunoprecipitation (IP) of CRYAB by using a monoclonal anti-CRYAB antibodies (Negative control: IP without adding anti-CRYAB antibody.). For each experiment, 500ul of DDK tagged CRYAB overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-CRYAB antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Azide-and-BSA-Free-Immunoprecipitation-NBP2-70454-img0007.jpg)
![Western Blot: PUMA Antibody (SR42-09) [NBP2-67326] - Analysis of PUMA on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: K562 cell lysate](http://images.novusbio.com/fullsize/PUMA-Antibody-SR42-09-Western-Blot-NBP2-67326-img0008.jpg "Western Blot: PUMA Antibody (SR42-09) [NBP2-67326] - Analysis of PUMA on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: K562 cell lysate")
![Immunocytochemistry/Immunofluorescence: PUMA Antibody (SR42-09) [NBP2-67326] - Staining PUMA in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PUMA-Antibody-SR42-09-Immunocytochemistry-Immunofluorescence-NBP2-67326-img0003.jpg)
![Immunohistochemistry-Paraffin: PUMA Antibody (SR42-09) [NBP2-67326] - Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-PUMA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BS](http://images.novusbio.com/fullsize/PUMA-Antibody-SR42-09-Immunohistochemistry-Paraffin-NBP2-67326-img0009.jpg)
![Flow Cytometry: PUMA Antibody (SR42-09) [NBP2-67326] - Analysis of Hela cells with PUMA antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/PUMA-Antibody-SR42-09-Flow-Cytometry-NBP2-67326-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PUMA Antibody (SR42-09) [NBP2-67326] - Staining PUMA in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PUMA-Antibody-SR42-09-Immunocytochemistry-Immunofluorescence-NBP2-67326-img0002.jpg)
![Immunohistochemistry-Paraffin: PUMA Antibody (SR42-09) [NBP2-67326] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-PUMA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PUMA-Antibody-SR42-09-Immunohistochemistry-Paraffin-NBP2-67326-img0004.jpg)
![Immunohistochemistry-Paraffin: PUMA Antibody (SR42-09) [NBP2-67326] - Analysis of paraffin-embedded human gastric carcimnoma tissue using anti-PUMA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PUMA-Antibody-SR42-09-Immunohistochemistry-Paraffin-NBP2-67326-img0005.jpg)
![Immunohistochemistry-Paraffin: PUMA Antibody (SR42-09) [NBP2-67326] - Analysis of paraffin-embedded mouse small intestine tissue using anti-PUMA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PUMA-Antibody-SR42-09-Immunohistochemistry-Paraffin-NBP2-67326-img0006.jpg)
![Immunohistochemistry-Paraffin: PUMA Antibody (SR42-09) [NBP2-67326] - Analysis of paraffin-embedded mouse stomach tissue using anti-PUMA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PUMA-Antibody-SR42-09-Immunohistochemistry-Paraffin-NBP2-67326-img0007.jpg)
![Western Blot: MKP-1/DUSP1 Antibody (JJ0930) [NBP2-67909] - Analysis of MKP-1/DUSP1 on SK-BR-3 cell tissue lysates with Rabbit anti-MKP-1/DUSP1 antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size:39 kDa Observed band size: 39 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/MKP-1-DUSP1-Antibody-JJ0930-Western-Blot-NBP2-67909-img0005.jpg "Western Blot: MKP-1/DUSP1 Antibody (JJ0930) [NBP2-67909] - Analysis of MKP-1/DUSP1 on SK-BR-3 cell tissue lysates with Rabbit anti-MKP-1/DUSP1 antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size:39 kDa Observed band size: 39 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: MKP-1/DUSP1 Antibody (JJ0930) [NBP2-67909] - Analysis of paraffin-embedded mouse lung tissue using anti-DUSP1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MKP-1-DUSP1-Antibody-JJ0930-Immunohistochemistry-Paraffin-NBP2-67909-img0003.jpg)
![Flow Cytometry: MKP-1/DUSP1 Antibody (JJ0930) [NBP2-67909] - Analysis of HepG2 cells with DUSP1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/MKP-1-DUSP1-Antibody-JJ0930-Flow-Cytometry-NBP2-67909-img0001.jpg)
![Western Blot: MKP-1/DUSP1 Antibody (JJ0930) [NBP2-67909] - Analysis of DUSP1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: NIH/3T3 cell lysateLane 2: Jurkat cell lysate Lane 3: mouse spleen tissue lysate](http://images.novusbio.com/fullsize/MKP-1-DUSP1-Antibody-JJ0930-Western-Blot-NBP2-67909-img0004.jpg)
![Immunohistochemistry-Paraffin: MKP-1/DUSP1 Antibody (JJ0930) [NBP2-67909] - Analysis of paraffin-embedded human lung cancer tissue using anti-DUSP1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MKP-1-DUSP1-Antibody-JJ0930-Immunohistochemistry-Paraffin-NBP2-67909-img0002.jpg)
![Western Blot: Lamin A + C Antibody [NBP1-85550] - Lamin A Antibody [NBP1-85550] - Analysis in human cell lines PC-3 and HEK293. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.](http://images.novusbio.com/fullsize/Lamin-A-Antibody-Western-Blot-NBP1-85550-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Lamin A + C Antibody [NBP1-85550] - Lamin A Antibody [NBP1-85550] - Analysis in human cell lines PC-3 and HEK293. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Lamin A Antibody [NBP1-85550] - Staining of human fallopian tube shows moderate positivity in nuclear membrane in glandular cells.](http://images.novusbio.com/fullsize/Lamin-A-Antibody-Immunohistochemistry-Paraffin-NBP1-85550-img0013.jpg)
![Immunohistochemistry-Paraffin: Lamin A Antibody [NBP1-85550] - Staining of human prostate shows strong positivity in nuclear membrane in smooth muscle cells and glandular cells.](http://images.novusbio.com/fullsize/Lamin-A-Antibody-Immunohistochemistry-Paraffin-NBP1-85550-img0011.jpg)
![Immunohistochemistry-Paraffin: Lamin A Antibody [NBP1-85550] - Staining of human stomach shows strong positivity in nuclear membrane in glandular cells.](http://images.novusbio.com/fullsize/Lamin-A-Antibody-Immunohistochemistry-Paraffin-NBP1-85550-img0012.jpg)
![Immunohistochemistry-Paraffin: Lamin A Antibody [NBP1-85550] - Staining of human skin shows strong positivity in nuclear membrane in squamous epithelial cells.](http://images.novusbio.com/fullsize/Lamin-A-Antibody-Immunohistochemistry-Paraffin-NBP1-85550-img0014.jpg)
![Western Blot: Rabbit Polyclonal Lamin A + C Antibody [NBP1-85550] - Western blot showing over-expression of lamin A/C tagged with mKate, as well as endogenous lamin A/C, in zebrafish embryos. Actin used as loading control. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-85550_rabbit-polyclonal-lamin-a-c-antibody-western-blot-722024124819..jpg)
![Immunohistochemistry-Paraffin: PP2A alpha Antibody (HL1447) - Azide and BSA Free [NBP3-25611] - PP2A alpha antibody [HL1447] detects PP2A alpha protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded cat kidney. PP2A alpha stained by PP2A alpha antibody [HL1447] (NBP3-25611) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25611_rabbit-pp2a-alpha-mab-hl1447-azide-and-bsa-free-2120249374979.jpg "Immunohistochemistry-Paraffin: PP2A alpha Antibody (HL1447) - Azide and BSA Free [NBP3-25611] - PP2A alpha antibody [HL1447] detects PP2A alpha protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded cat kidney. PP2A alpha stained by PP2A alpha antibody [HL1447] (NBP3-25611) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![<b>Genetic Strategies Validation. </b>Western Blot: PP2A alpha Antibody (HL1447) - Azide and BSA Free [NBP3-25611] - Non-transfected (-) and transfected (+) PP2A alpha shRNA-transfected 293T (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PP2A alpha antibody [HL1447] (NBP3-25611) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25611_rabbit-pp2a-alpha-mab-hl1447-azide-and-bsa-free-212024941484.jpg)
![Immunohistochemistry-Paraffin: PP2A alpha Antibody (HL1447) - Azide and BSA Free [NBP3-25611] - PP2A alpha antibody [HL1447] detects PP2A alpha protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded dog kidney. PP2A alpha stained by PP2A alpha antibody [HL1447] (NBP3-25611) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25611_rabbit-pp2a-alpha-mab-hl1447-azide-and-bsa-free-2120249342754.jpg)
![Immunohistochemistry-Paraffin: PP2A alpha Antibody (HL1447) - Azide and BSA Free [NBP3-25611] - PP2A alpha antibody [HL1447] detects PP2A alpha protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. PP2A alpha stained by PP2A alpha antibody [HL1447] (NBP3-25611) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25611_rabbit-pp2a-alpha-mab-hl1447-azide-and-bsa-free-212024935555.jpg)
![Immunocytochemistry/Immunofluorescence: PP2A alpha Antibody (HL1447) - Azide and BSA Free [NBP3-25611] - PP2A alpha antibody [HL1447] detects PP2A alpha protein by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PP2A alpha stained by PP2A alpha antibody [HL1447] (NBP3-25611) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25611_rabbit-pp2a-alpha-mab-hl1447-azide-and-bsa-free-2120249362724.jpg)
![Western Blot: PP2A alpha Antibody (HL1447) - Azide and BSA Free [NBP3-25611] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PP2A alpha antibody [HL1447] (NBP3-25611) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25611_rabbit-pp2a-alpha-mab-hl1447-azide-and-bsa-free-2120249413318.jpg)
![Western Blot: PP2A alpha Antibody (HL1447) - Azide and BSA Free [NBP3-25611] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PP2A alpha antibody [HL1447] (NBP3-25611) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25611_rabbit-pp2a-alpha-mab-hl1447-azide-and-bsa-free-212024938310.jpg)
![Immunohistochemistry-Paraffin: PP2A alpha Antibody (HL1447) - Azide and BSA Free [NBP3-25611] - PP2A alpha antibody [HL1447] detects PP2A alpha protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. PP2A alpha stained by PP2A alpha antibody [HL1447] (NBP3-25611) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25611_rabbit-pp2a-alpha-mab-hl1447-azide-and-bsa-free-2120249403842.jpg)
![Western Blot: PP2A alpha Antibody (HL1447) - Azide and BSA Free [NBP3-25611] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PP2A alpha antibody [HL1447] (NBP3-25611) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25611_rabbit-pp2a-alpha-mab-hl1447-azide-and-bsa-free-2120249374958.jpg)