| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-102SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-201SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89236-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56485SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92178-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61775-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97747-50ul | |||
![Immunohistochemistry: Neurokinin B Antibody - BSA Free [NB300-201] - Immunofluorescence photomicrograph showing that the expression of Neurokinin B in the arcuate nucleus (ARC). Scale bars: 20 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32921318/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Neurokinin-B-Antibody---BSA-Free-Immunohistochemistry-NB300-201-img0009.jpg "<b>Biological Strategies Validation. </b>Immunohistochemistry: Neurokinin B Antibody - BSA Free [NB300-201] - Immunofluorescence photomicrograph showing that the expression of Neurokinin B in the arcuate nucleus (ARC). Scale bars: 20 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32921318/) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Neurokinin B Antibody [NB300-201] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse brain using Neurokinin B antibody at 1:200 dilution. The antibody depicted an expected strong cytoplasmic staining in the neuronal cells.](http://images.novusbio.com/fullsize/Neurokinin-B-Antibody-Immunohistochemistry-Paraffin-NB300-201-img0005.jpg)
![Immunohistochemistry-Paraffin: Neurokinin B Antibody [NB300-201] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse brain using Neurokinin B antibody at 1:100 dilution. The antibody showed a specific cytoplasmic staining in the Purkinje cells and some granular neurons from the granular layer with weak signal in the fibers within the molecular layer of the section.](http://images.novusbio.com/fullsize/Neurokinin-B-Antibody-Immunohistochemistry-Paraffin-NB300-201-img0006.jpg)
![Immunohistochemistry: Neurokinin B Antibody [NB300-201] - IF analysis of Neurokinin B in mouse hypothalamus. Image courtesy of an anonymous customer review.](http://images.novusbio.com/fullsize/Neurokinin-B-Antibody-Immunohistochemistry-NB300-201-img0007.jpg)
![Immunohistochemistry: Neurokinin B Antibody [NB300-201] - Detection of Neurokinin B in rat brain tissue (Alexa 488 stain), using NB 300-201.](http://images.novusbio.com/fullsize/Neurokinin-B-Antibody-Immunohistochemistry-NB300-201-img0008.jpg)
![Immunocytochemistry/ Immunofluorescence: Neurokinin B Antibody - BSA Free [NB300-201] - Troxerutin reduced the expression of Kisspepetin1 & Neurokinin B in the ARC. a Immunofluorescence photomicrograph showing that the expression of Kisspeptin1 & NKB in the arcuate nucleus (ARC). Scale bars:20 μm. b A lower magnification image showing the whole ARC & median eminence. Rectangular frame a & b represent ARC & median eminence (ME) analyzed in Fig. 5a & Fig. 6a, respectively. Scale bars:200 μm. c Quantitative analysis of integral optical density (IOD) of Kisspeptin1 & NKB in the ARC. P values were determined by one-way ANOVA with Tukey’s multiple comparison test & data were presented as means±s.e.m. n = 4 per group. **P < 0.01, ***P < 0.001vs. sham group; #P < 0.05, ##P < 0.01 vs. PCOS group Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32921318), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-201_rabbit-polyclonal-neurokinin-b-antibody-310202415175279.jpg)
![Immunohistochemistry: Neurokinin B Antibody - BSA Free [NB300-201] - Characterization of Kiss1-null mice.(A-C) Immunofluorescence for kisspeptin (A,B) & NKB (C) in the ARN of wild-type (A) & Kiss1-null (B,C) female mice. (D) Cell-attached recordings showing the effects of 100 nM NKB on firing of KNDy neurons in acute brain slices prepared from female heterozygous Kiss1Cre/+ and homozygous (null) Kiss1Cre/Cre;Rosa26-tdT mice. (E) Mean ± SEM changes in KNDy neuron firing evoked by 100 nM NKB in heterozygous Kiss1Cre/+and homozygous (null) Kiss1Cre/Cre;Rosa26-tdT mice. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33464205), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-201_rabbit-polyclonal-neurokinin-b-antibody-310202415291957.jpg)
![Immunohistochemistry-Paraffin: ADNP Antibody [NBP1-89236] - Analysis in human testis and liver tissues using NBP1-89236 antibody. Corresponding ADNP RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ADNP-Antibody-Immunohistochemistry-Paraffin-NBP1-89236-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ADNP Antibody [NBP1-89236] - Analysis in human testis and liver tissues using NBP1-89236 antibody. Corresponding ADNP RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: ADNP Antibody [NBP1-89236] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/ADNP-Antibody-Western-Blot-NBP1-89236-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: ADNP Antibody [NBP1-89236] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ADNP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89236-img0009.jpg)
![Immunohistochemistry-Paraffin: ADNP Antibody [NBP1-89236] - Staining of human prostate shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/ADNP-Antibody-Immunohistochemistry-Paraffin-NBP1-89236-img0013.jpg)
![Western Blot: ADNP Antibody [NBP1-89236] - Analysis in human cell line SH-SY5Y.](http://images.novusbio.com/fullsize/ADNP-Antibody-Western-Blot-NBP1-89236-img0007.jpg)
![Immunohistochemistry-Paraffin: ADNP Antibody [NBP1-89236] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/ADNP-Antibody-Immunohistochemistry-Paraffin-NBP1-89236-img0011.jpg)
![Immunohistochemistry-Paraffin: ADNP Antibody [NBP1-89236] - Staining of human endometrium shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/ADNP-Antibody-Immunohistochemistry-Paraffin-NBP1-89236-img0012.jpg)
![Immunohistochemistry-Paraffin: ADNP Antibody [NBP1-89236] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/ADNP-Antibody-Immunohistochemistry-Paraffin-NBP1-89236-img0014.jpg)
![Western Blot: CRHR2/CRF2 Antibody [NB100-56485] - Analysis of CRHR2 in human brain lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. Both of the isoforms detected by this antibody.](http://images.novusbio.com/fullsize/CRHR2-CRF2-Antibody-Western-Blot-NB100-56485-img0002.jpg "Western Blot: CRHR2/CRF2 Antibody [NB100-56485] - Analysis of CRHR2 in human brain lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. Both of the isoforms detected by this antibody.")
![Simple Western: CRHR2/CRF2 Antibody [NB100-56485] - Shows a specific band for Corticotropin Releasing Factor 2 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/CRHR2-CRF2-Antibody-Simple-Western-NB100-56485-img0003.jpg)
![Western Blot: GluR2 Antibody (JU20-43) [NBP2-75510] - Analysis of GluR2 on rat brain lysates with Rabbit anti-GluR2 antibody at 1/500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 99 kDa Observed band size: 90 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/GluR2-Antibody-JU20-43-Western-Blot-NBP2-75510-img0005.jpg "Western Blot: GluR2 Antibody (JU20-43) [NBP2-75510] - Analysis of GluR2 on rat brain lysates with Rabbit anti-GluR2 antibody at 1/500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 99 kDa Observed band size: 90 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: GluR2 Antibody (JU20-43) [NBP2-75510] - Staining GluR2 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/GluR2-Antibody-JU20-43-Immunocytochemistry-Immunofluorescence-NBP2-75510-img0002.jpg)
![Immunohistochemistry-Paraffin: GluR2 Antibody (JU20-43) [NBP2-75510] - Analysis of paraffin-embedded mouse brain tissue with Rabbit anti-GluR2 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/GluR2-Antibody-JU20-43-Immunohistochemistry-Paraffin-NBP2-75510-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: GluR2 Antibody (JU20-43) [NBP2-75510] - Staining GluR2 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/GluR2-Antibody-JU20-43-Immunocytochemistry-Immunofluorescence-NBP2-75510-img0001.jpg)
![Immunohistochemistry-Paraffin: GluR2 Antibody (JU20-43) [NBP2-75510] - Analysis of paraffin-embedded mouse brain tissue using anti-GluR2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GluR2-Antibody-JU20-43-Immunohistochemistry-Paraffin-NBP2-75510-img0003.jpg)
![Immunohistochemistry-Paraffin: GluR2 Antibody (JU20-43) [NBP2-75510] - Analysis of paraffin-embedded rat brain tissue using anti-GluR2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GluR2-Antibody-JU20-43-Immunohistochemistry-Paraffin-NBP2-75510-img0004.jpg)
![Immunohistochemistry-Paraffin: GluR2 Antibody (JU20-43) [NBP2-75510] - Analysis of paraffin-embedded rat brain tissue with Rabbit anti-GluR2 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/GluR2-Antibody-JU20-43-Immunohistochemistry-Paraffin-NBP2-75510-img0006.jpg)
![Western Blot: GNB1 Antibody [NBP2-16699] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/GNB1-Antibody-Western-Blot-NBP2-16699-img0002.jpg "Western Blot: GNB1 Antibody [NBP2-16699] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:10000.")
![Immunohistochemistry-Paraffin: GNB1 Antibody [NBP2-16699] - Mouse testis. GNB1 antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/GNB1-Antibody-Immunohistochemistry-Paraffin-NBP2-16699-img0008.jpg)
![Immunohistochemistry-Paraffin: GNB1 Antibody [NBP2-16699] - Human lung papillory adenocarcinoma, using GNB1antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/GNB1-Antibody-Immunohistochemistry-Paraffin-NBP2-16699-img0003.jpg)
![Immunoprecipitation: GNB1 Antibody [NBP2-16699] - Immunoprecipitation of GNB1 protein from 293T whole cell extracts using 5 ug of GNB1 antibody. Western blot analysis was performed using GNB1 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/GNB1-Antibody-Immunoprecipitation-NBP2-16699-img0005.jpg)
![Immunohistochemistry Whole-Mount:GNB1 Antibody [NBP2-16699] -Analysis (whole mount) of zebrafish embryo, using GNB1 antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/antibody/nbp2-16699_rabbit-polyclonal-gnb1-antibody-immunohistochemistry-whole-mount-310202481127..jpg)
![GNB1 antibody detects GNB1 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GNB1 protein stained by GNB1 antibody (NBP2-16699) diluted at 1:500.Red: FIS1, a mitochondria marker, stained by FIS1 antibody [GT9810] diluted at 1:20000.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-16699_rabbit-polyclonal-gnb1-antibody-immunocytochemistry-immunofluorescence-26112025194810.jpg)
![Immunocytochemistry/Immunofluorescence: Neurokinin B Antibody [NBP1-92178] - Staining of human cell line U-2 OS shows localization to vesicles.](http://images.novusbio.com/fullsize/Neurokinin-B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-92178-img0004.jpg "Immunocytochemistry/Immunofluorescence: Neurokinin B Antibody [NBP1-92178] - Staining of human cell line U-2 OS shows localization to vesicles.")
![Immunohistochemistry-Paraffin: Neurokinin B Antibody [NBP1-92178] - Staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.](http://images.novusbio.com/fullsize/Neurokinin-B-Antibody-Immunohistochemistry-Paraffin-NBP1-92178-img0002.jpg)
![Immunohistochemistry-Paraffin: Neurokinin B Antibody [NBP1-92178] -Staining of human Placenta shows weak cytoplasmic positivity in syncytiotrophoblast.](http://images.novusbio.com/fullsize/antibody/nbp1-92178_rabbit-polyclonal-neurokinin-b-antibody-immunohistochemistry-paraffin-22120258449..png)
![Immunohistochemistry-Paraffin: Neurokinin B Antibody [NBP1-92178] -Staining of human Liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/antibody/nbp1-92178_rabbit-polyclonal-neurokinin-b-antibody-immunohistochemistry-paraffin-22120258539..png)
![Immunocytochemistry/Immunofluorescence: Neurokinin B Antibody [NBP1-92178] -Staining of Mouse brain shows moderate positivity in cells in molecular layer in the cerebellum.](http://images.novusbio.com/fullsize/antibody/nbp1-92178_rabbit-polyclonal-neurokinin-b-antibody-immunocytochemistry-immunofluorescence-22120258647..png)
![Western Blot: GluR2 Antibody (9R1X2) [NBP3-15370] - Analysis of extracts of various cell lines, using GluR2 Rabbit mAb (NBP3-15370) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/GluR2-Antibody-9R1X2-Western-Blot-NBP3-15370-img0006.jpg "Western Blot: GluR2 Antibody (9R1X2) [NBP3-15370] - Analysis of extracts of various cell lines, using GluR2 Rabbit mAb (NBP3-15370) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: GluR2 Antibody (9R1X2) [NBP3-15370] - Immunofluorescence analysis of SH-SY5Y cells using GluR2 Rabbit mAb (NBP3-15370) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/GluR2-Antibody-9R1X2-Immunocytochemistry-Immunofluorescence-NBP3-15370-img0003.jpg)
![Immunohistochemistry: GluR2 Antibody (9R1X2) [NBP3-15370] - Immunofluorescence analysis of mouse brain using GluR2 Rabbit mAb (NBP3-15370) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/GluR2-Antibody-9R1X2-Immunohistochemistry-NBP3-15370-img0005.jpg)
![Immunohistochemistry-Paraffin: GluR2 Antibody (9R1X2) [NBP3-15370] - Rat brain using GluR2 Rabbit mAb (NBP3-15370) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/GluR2-Antibody-9R1X2-Immunohistochemistry-Paraffin-NBP3-15370-img0001.jpg)
![Immunohistochemistry-Paraffin: GluR2 Antibody (9R1X2) [NBP3-15370] - Mouse brain using GluR2 Rabbit mAb (NBP3-15370) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/GluR2-Antibody-9R1X2-Immunohistochemistry-Paraffin-NBP3-15370-img0002.jpg)
![Immunohistochemistry: GluR2 Antibody (9R1X2) [NBP3-15370] - Immunofluorescence analysis of rat brain using GluR2 Rabbit mAb (NBP3-15370) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/GluR2-Antibody-9R1X2-Immunohistochemistry-NBP3-15370-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: GluR2 Antibody (9R1X2) [GluR2] - Confocal imaging of paraffin-embedded Rat brain tissue using GluR2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15370_rabbit-glur2-mab-9r1x2-52202517162341.jpg)
![Immunocytochemistry/ Immunofluorescence: GluR2 Antibody (9R1X2) [GluR2] - Confocal imaging of paraffin-embedded Mouse brain tissue using GluR2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15370_rabbit-glur2-mab-9r1x2-52202517172841.jpg)
![Western Blot: GluR2 Antibody (7A7A3) [NBP2-61775] - Analysis using GRIA2 mAb against human GRIA2 (AA: 35-175) recombinant protein. (Expected MW is 41.9 kDa)](http://images.novusbio.com/fullsize/GluR2-Antibody-7A7A3-Western-Blot-NBP2-61775-img0004.jpg "Western Blot: GluR2 Antibody (7A7A3) [NBP2-61775] - Analysis using GRIA2 mAb against human GRIA2 (AA: 35-175) recombinant protein. (Expected MW is 41.9 kDa)")
![Immunohistochemistry-Paraffin: GluR2 Antibody (7A7A3) [NBP2-61775] - Analysis of rectum cancer tissues using GRIA2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/GluR2-Antibody-7A7A3-Immunohistochemistry-NBP2-61775-img0003.jpg)
![Flow Cytometry: GluR2 Antibody (7A7A3) [NBP2-61775] - Analysis of SK-N-SH cells using GRIA2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/GluR2-Antibody-7A7A3-Flow-Cytometry-NBP2-61775-img0002.jpg)
![Western Blot: GluR2 Antibody (7A7A3) [NBP2-61775] - Analysis using GRIA2 mAb against HEK293 (1) and GRIA2 (AA: 35-175)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/GluR2-Antibody-7A7A3-Western-Blot-NBP2-61775-img0005.jpg)
![ELISA: GluR2 Antibody (7A7A3) [NBP2-61775] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/GluR2-Antibody-7A7A3-ELISA-NBP2-61775-img0001.jpg)
![Western Blot: ADNP Antibody [NBP2-97747] - Anti-ADNP rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: U-251 MG Whole Cell Lysate. Lysates/proteins at 30 ug per lane.](http://images.novusbio.com/fullsize/ADNP-Antibody-Western-Blot-NBP2-97747-img0003.jpg "Western Blot: ADNP Antibody [NBP2-97747] - Anti-ADNP rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: U-251 MG Whole Cell Lysate. Lysates/proteins at 30 ug per lane.")
![Immunocytochemistry/Immunofluorescence: ADNP Antibody [NBP2-97747] - Immunofluorescence staining of ADNP in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human ADNP polyclonal antibody (dilution ratio 1:300) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG Secondary antibody (red). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/ADNP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-97747-img0001.jpg)
![Immunoprecipitation: ADNP Antibody [NBP2-97747] - ADNP was immunoprecipitated using: Lane A: 0.5 mg U-251MG Whole Cell Lysate. 4 ul anti-ADNP rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-ADNP rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 122 kDa. Observed band size: 150 kDa.](http://images.novusbio.com/fullsize/ADNP-Antibody-Immunoprecipitation-NBP2-97747-img0004.jpg)
