| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-58345SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47946-0.1mg | |||
| IHC-Prediluted | NBP2-48095 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59320-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-74570SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89152-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-96431-0.025mg | |||
![Western Blot: CDR2 Antibody [NB110-58345] - Cdr2 expressed during mitosis, ubiquitinated and degraded during mitotic exit. Upper left, flow cytometry of G1/S- and G2/M-arrested HEK293s. Upper right, western blots of HEK293 G1/S and G2/M extracts probed with anti-cdr2 (NB110; top), cyclinB1 (middle) and gamma-tubulin (bottom). Bottom panel, cdr2 and cyclin B1 protein quantitation, normalized here and in D to gamma--tubulin levels; *p<0.001. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0010045), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CDR2-Antibody-Western-Blot-NB110-58345-img0009.jpg "Western Blot: CDR2 Antibody [NB110-58345] - Cdr2 expressed during mitosis, ubiquitinated and degraded during mitotic exit. Upper left, flow cytometry of G1/S- and G2/M-arrested HEK293s. Upper right, western blots of HEK293 G1/S and G2/M extracts probed with anti-cdr2 (NB110; top), cyclinB1 (middle) and gamma-tubulin (bottom). Bottom panel, cdr2 and cyclin B1 protein quantitation, normalized here and in D to gamma--tubulin levels; *p<0.001. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0010045), licensed under a CC-BY license.")
![Immunohistochemistry: CDR2 Antibody [NB110-58345] - Purkinje neurons of the cerebellum.](http://images.novusbio.com/fullsize/CDR2-Antibody-Immunohistochemistry-NB110-58345-img0004.jpg)
![Flow Cytometry: CDR2 Antibody [NB110-58345] - Analysis using the DyLight 488 conjugate of NB110-58345. Thymus epithelial cells CDR2. Image from verified customer review.](http://images.novusbio.com/fullsize/CDR2-Antibody-Flow-Cytometry-NB110-58345-img0008.jpg)
![Western Blot: CDR2 Antibody [NB110-58345] - Analysis of CDR2 antibody on HeLa whole cell.](http://images.novusbio.com/fullsize/CDR2-Antibody-Western-Blot-NB110-58345-img0006.jpg)
![Immunohistochemistry-Paraffin: Glutathione S-Transferase pi 1/GSTP1 Antibody [NBP2-16756] - U87 xenograft, using GSTP1antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Glutathione-S-Transferase-pi-1-GSTP1-Antibody-Immunohistochemistry-Paraffin-NBP2-16756-img0014.jpg)
![GSTP1 antibody detects GSTP1 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GSTP1 protein stained by GSTP1 antibody (NBP2-16756) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-16756_rabbit-polyclonal-glutathione-s-transferase-pi-1-gstp1-antibody-immunocytochemistry-immunofluorescence-2611202518283519.jpg)
![Western Blot: Cytokeratin 19 Antibody (A53-B/A2.26) - Azide and BSA Free [NBP2-33134] - Western Blot Analysis of human Prostate Cancer PC-3 cell lysate Cytokeratin 19 Antibody (A53-B/A2.26).](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Azide-and-BSA-Free-Western-Blot-NBP2-33134-img0007.jpg "Western Blot: Cytokeratin 19 Antibody (A53-B/A2.26) - Azide and BSA Free [NBP2-33134] - Western Blot Analysis of human Prostate Cancer PC-3 cell lysate Cytokeratin 19 Antibody (A53-B/A2.26).")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody (A53-B/A2.26) - Azide and BSA Free [NBP2-33134] - Immunofluorescence Analysis of MCF-7 cells labeling Cytokeratin 19 with Cytokeratin 19 Antibody (A53-B/A2.26) followed by Goat anti-Mouse IgG-CF488 (Cyan). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-33134-img0005.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (A53-B/A2.26) - Azide and BSA Free [NBP2-33134] - Formalin-paraffin colon carcinoma stained with Cytokeratin 19 Ab (A53-B/A2.26).](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-33134-img0008.jpg)
![Western Blot: Cytokeratin 19 Antibody (A53-B/A2.26) - Azide and BSA Free [NBP2-33134] - Western blot 1) Hep-G2 and 2) MCF-7 lysate using of Cytokeratin 19 Antibody (A53-B/A2.26).](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Azide-and-BSA-Free-Western-Blot-NBP2-33134-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody (A53-B/A2.26) - Azide and BSA Free [NBP2-33134] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-Cytokeratin 19 (A53-B/A2.26) conjugated to Alexa Fluor 488 [NBP2-33134AF488] at 10ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the Alexa Fluor 488 version of this antibody.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-33134-img0004.jpg)
![Immunohistochemistry-Frozen: Cytokeratin 19 Antibody (A53-B/A2.26) - Azide and BSA Free [NBP2-33134] - Breast cancer tissue stained for Cytokeratin 19 (red) counter stained with DAPI (blue). The Janelia-549 conjugate of this antibody was used. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Azide-and-BSA-Free-Immunohistochemistry-Frozen-NBP2-33134-img0001.jpg)
![Simple Western: Cytokeratin 19 Antibody (A53-B/A2.26) - Azide and BSA Free [NBP2-33134] - Simple Western lane view shows a specific band for Cytokeratin 19 in 0.2 mg/ml of HeLa lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Azide-and-BSA-Free-Simple-Western-NBP2-33134-img0002.jpg)
![Simple Western: Cytokeratin 19 Antibody (A53-B/A2.26) - Azide and BSA Free [NBP2-33134] - Electropherogram image of the corresponding Simple Western lane. Cytokeratin 19 antibody was used at 10 ug/ml dilution of HeLa lysates(s) respectively.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Azide-and-BSA-Free-Simple-Western-NBP2-33134-img0003.jpg)
![Western Blot: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Western Blot Analysis of human lung lysate using Cytokeratin 19 Antibody (KRT19/800).](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-800-Western-Blot-NBP2-44827-img0012.jpg "Western Blot: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Western Blot Analysis of human lung lysate using Cytokeratin 19 Antibody (KRT19/800).")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Immunofluorescence Analysis of MeOH-fixed MCF-7 cells labeling CK19 using Cytokeratin 19 Antibody (KRT19/800) followed by Goat anti-Mouse IgG-CF488 (Cyan). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-800-Immunocytochemistry-Immunofluorescence-NBP2-44827-img0010.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Formalin-fixed, paraffin-embedded Rat Colon stained with Cytokeratin 19 Antibody (KRT19/800).](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-800-Immunohistochemistry-Paraffin-NBP2-44827-img0011.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Human Cervical Carcinoma stained with Cytokeratin 19 Monoclonal Antibody (KRT19/800)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-800-Immunohistochemistry-Paraffin-NBP2-44827-img0002.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Human Endometrial Carcinoma stained with Cytokeratin 19 Monoclonal Antibody (KRT19/800)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-800-Immunohistochemistry-Paraffin-NBP2-44827-img0003.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Human Pancreas stained with Cytokeratin 19 Monoclonal Antibody (KRT19/800)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-800-Immunohistochemistry-Paraffin-NBP2-44827-img0005.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Rat Colon stained with Cytokeratin 19 Monoclonal Antibody (KRT19/800)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-800-Immunohistochemistry-Paraffin-NBP2-44827-img0006.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Rat Lung stained with Cytokeratin 19 Monoclonal Antibody (KRT19/800)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-800-Immunohistochemistry-Paraffin-NBP2-44827-img0007.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Rat Stomach stained with Cytokeratin 19 Monoclonal Antibody (KRT19/800)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-800-Immunohistochemistry-Paraffin-NBP2-44827-img0008.jpg)
![Western Blot: SUR1 Antibody (S289-16) [NBP2-59320] - Western Blot analysis of Rat Brain Membrane showing detection of ~160 kDa SUR1 protein using Mouse Anti-SUR1 Monoclonal Antibody, Clone S289-16 (NBP2-59320). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-SUR1 Monoclonal Antibody (NBP2-59320) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~160 kDa.](http://images.novusbio.com/fullsize/SUR1-Antibody-S289-16-Western-Blot-NBP2-59320-img0007.jpg "Western Blot: SUR1 Antibody (S289-16) [NBP2-59320] - Western Blot analysis of Rat Brain Membrane showing detection of ~160 kDa SUR1 protein using Mouse Anti-SUR1 Monoclonal Antibody, Clone S289-16 (NBP2-59320). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-SUR1 Monoclonal Antibody (NBP2-59320) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~160 kDa.")
![Immunocytochemistry/Immunofluorescence: SUR1 Antibody (S289-16) [NBP2-59320] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SUR1 Monoclonal Antibody, Clone S289-16 (NBP2-59320). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-SUR1 Monoclonal Antibody (NBP2-59320) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SUR1 Antibody (D) Composite.](http://images.novusbio.com/fullsize/SUR1-Antibody-S289-16-Immunocytochemistry-Immunofluorescence-NBP2-59320-img0008.jpg)
![Western Blot: SUR1 Antibody (S289-16) [NBP2-59320] - Analysis of mouse pancreatic islets. Lane 1: Molecular Weight Ladder. Lanes 2-5: Samples from pooled pancreatic islets. Load: ~10ug. Primary Antibody: Anti-SUR1 Monoclonal Antibody at 1:200 for ~16hrs at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1hr RT. Image from verified customer review.](http://images.novusbio.com/fullsize/SUR1-Antibody-S289-16-Western-Blot-NBP2-59320-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: SUR1 Antibody (S289-16) [NBP2-59320] - Analysis using Mouse Anti-SUR1 Monoclonal Antibody, Clone S289-16. Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SUR1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) SUR1 Antibody (D) Composite.](http://images.novusbio.com/fullsize/SUR1-Antibody-S289-16-Immunocytochemistry-Immunofluorescence-NBP2-59320-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: SUR1 Antibody (S289-16) [NBP2-59320] - Glial fibrillary acidic protein (GFAP)-positive specimens from human contusion- traumatic brain injury (TBI) exhibit sulfonylurea receptor 1 (SUR1) expression in astrocytes.Double immunolabeling for SUR1 (red) and GFAP (green) showed astrocyte expression of SUR1; merged images confirm co-localization (yellow); in situ hybridization of the same tissue section for Abcc8 messenger RNA showed positive signal co-localized with GFAP-positive, SUR1-expressing astrocytes; arrowheads point to cells with all three signals. Image collected and cropped by CiteAb from the following publication (//www.liebertpub.com/doi/10.1089/neu.2018.5986) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SUR1-Antibody-S289-16-Immunocytochemistry-Immunofluorescence-NBP2-59320-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: SUR1 Antibody (S289-16) [NBP2-59320] - Glial fibrillary acidic protein (GFAP)–positive specimens from human contusion- traumatic brain injury (TBI) exhibit KIR6.2 expression in astrocytes. (A) Immunolabeling for KIR6.2 showed sparse immunoreactivity in the control specimen (CTR) vs. widespread expression in a GFAP-positive specimen from contusion-TBI. (B) Double immunolabeling for GFAP (red) & KIR6.2 (green) showed astrocyte expression of KIR6.2; merged images confirm co-localization (yellow); in situ hybridization of the same tissue section for Kcnj11 messenger RNA showed positive signal co-localized with GFAP-positive, KIR6.2-expressing astrocytes; arrowheads point to cells with all three signals. (C) Double immunolabeling showed that KIR6.2 (red) & sulfonylurea receptor 1 (SUR1; green) were co-localized (yellow) in astrocytes. (D) ImmunoFRET for SUR1 (magenta) & KIR6.2 (red) showed co-assembly of SUR1-KIR6.2 heteromers (yellow pseudocolor) in astrocytes. (E) Double immunolabeling showed that KIR6.2 (green) & transient receptor potential cation channel subfamily M member 4 (TRPM4) (red) were co-localized in astrocytes. The findings illustrated are representative of all GFAP-positive specimens from eight cases of human contusion-TBI. (case #2, 11 days post-TBI; case #5, 1 day post-TBI) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30160201), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-59320_mouse-monoclonal-sur1-antibody-s289-16-310202416114773.jpg)
![Western Blot: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of CD34 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: TF-1 cell lysate Lane 2: Mouse lung tissue lysate](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Western-Blot-NBP2-67399-img0007.jpg "Western Blot: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of CD34 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: TF-1 cell lysate Lane 2: Mouse lung tissue lysate")
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - (G) CD34 + microvascular staining in EOC tissue. Correlation between HE4 serum levels and CD34 + area in patient EOC tissue (n = 14). **p < 0.005, ****p < 0.0005. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-020-65353-x), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0008.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded rat kidney tissue with Rabbit anti-CD34 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - Staining CD34 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - Staining CD34 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - Staining CD34 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0003.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human kidney tissue using anti-CD34 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0004.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded mouse kidney tissue using anti-CD34 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0006.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human tonsil tissue using anti-CD34 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0005.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human tonsil tissue labeling CD34 with Rabbit anti-CD34 antibody washed with PBS, and then probed with the primary antibody green) at 1/50 dilution overnight at 4 , washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0009.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-CD34 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0010.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human placenta tissue with Rabbit anti-CD34 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0011.jpg)
![Western Blot: CHT1 Antibody (62-2E8) [NB110-74570] - Western Blot Image of anti-CHT1 (62-2E8). Total protein from mouse brain was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with a 1:500 dilution of anti-CHT1 in 1% milk/TBST. Reactivity was detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/CHT1-Antibody-62-2E8-Western-Blot-NB110-74570-img0003.jpg "Western Blot: CHT1 Antibody (62-2E8) [NB110-74570] - Western Blot Image of anti-CHT1 (62-2E8). Total protein from mouse brain was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with a 1:500 dilution of anti-CHT1 in 1% milk/TBST. Reactivity was detected with an anti-mouse HRP secondary antibody using chemiluminescence.")
![Immunohistochemistry: CHT1 Antibody (62-2E8) [NB110-74570] - Staining of neurons in rat basal forebrain](http://images.novusbio.com/fullsize/CHT1-Antibody-62-2E8-Immunohistochemistry-NB110-74570-img0002.jpg)
![Western Blot: Glutathione Synthetase Antibody (JB95-33) [NBP2-75513] - Analysis of Glutathione Synthetase on different lysates using anti-Glutathione Synthetase antibody at 1/500 dilution.Positive control:Lane 1: SiHa Lane 2: Mouse colonLane 3: Rat liver](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-JB95-33-Western-Blot-NBP2-75513-img0008.jpg "Western Blot: Glutathione Synthetase Antibody (JB95-33) [NBP2-75513] - Analysis of Glutathione Synthetase on different lysates using anti-Glutathione Synthetase antibody at 1/500 dilution.Positive control:Lane 1: SiHa Lane 2: Mouse colonLane 3: Rat liver")
![Immunocytochemistry/Immunofluorescence: Glutathione Synthetase Antibody (JB95-33) [NBP2-75513] - Staining Glutathione Synthetase in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-JB95-33-Immunocytochemistry-Immunofluorescence-NBP2-75513-img0002.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody (JB95-33) [NBP2-75513] - Analysis of paraffin-embedded rat epididymis tissue using anti-Glutathione Synthetase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-JB95-33-Immunohistochemistry-Paraffin-NBP2-75513-img0007.jpg)
![Flow Cytometry: Glutathione Synthetase Antibody (JB95-33) [NBP2-75513] - Analysis of HepG2 cells with Glutathione Synthetase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-JB95-33-Flow-Cytometry-NBP2-75513-img0001.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody (JB95-33) [NBP2-75513] - Analysis of paraffin-embedded human tonsil tissue using anti-Glutathione Synthetase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-JB95-33-Immunohistochemistry-Paraffin-NBP2-75513-img0003.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody (JB95-33) [NBP2-75513] - Analysis of paraffin-embedded human colon cancer tissue using anti-Glutathione Synthetase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-JB95-33-Immunohistochemistry-Paraffin-NBP2-75513-img0004.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody (JB95-33) [NBP2-75513] - Analysis of paraffin-embedded human kidney tissue using anti-Glutathione Synthetase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-JB95-33-Immunohistochemistry-Paraffin-NBP2-75513-img0005.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody (JB95-33) [NBP2-75513] - Analysis of paraffin-embedded mouse colon tissue using anti-Glutathione Synthetase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-JB95-33-Immunohistochemistry-Paraffin-NBP2-75513-img0006.jpg)
![Western Blot: BIRC6 Antibody [NB110-40730] - Dual IAP-targeting ASOs knockdown BIRC6, cIAP1 or survivin proteins and lead to marked suppression of CRPC cell proliferation. Western blotting showing protein levels of BIRC6, cIAP1 and survivin in two CRPC cell lines (A) PC-3 cells and (B) C4-2 cells transfected with Mock or increasing dosages of scrambled ASO (Scrb), dASOs 6w2 and 6w5 for 72 hr. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/fulltext/2229), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/BIRC6-Antibody-Western-Blot-NB110-40730-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: BIRC6 Antibody [NB110-40730] - Dual IAP-targeting ASOs knockdown BIRC6, cIAP1 or survivin proteins and lead to marked suppression of CRPC cell proliferation. Western blotting showing protein levels of BIRC6, cIAP1 and survivin in two CRPC cell lines (A) PC-3 cells and (B) C4-2 cells transfected with Mock or increasing dosages of scrambled ASO (Scrb), dASOs 6w2 and 6w5 for 72 hr. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/fulltext/2229), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: BIRC6 Antibody [NB110-40730] - Treatments with dASOs resulted in significant lower viable tumor volume. Representative images of control ASO-6w2- and 6w5-treated PC-3 xenografts using H&E, and IHC staining of BIRC6, cleaved-caspase 3 and Ki-67. 20x magnification. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/fulltext/2229), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/BIRC6-Antibody-Immunohistochemistry-NB110-40730-img0005.jpg)
![Immunohistochemistry-Paraffin: BIRC6 Antibody [NB110-40730] - FFPE section of human metastatic lymph node. Antibody: Affinity purified rabbitanti-BIRC6/Apollon used at a dilution of 1:250. Detection: DAB](http://images.novusbio.com/fullsize/BIRC6-Antibody-Immunohistochemistry-Paraffin-NB110-40730-img0003.jpg)
![Immunohistochemistry-Paraffin: BIRC6 Antibody [NB110-40730] - FFPE section of human ovarian tumor. Antibody: Affinity purified rabbit antiBIRC6/Apollon used at a dilution of 1:250. Detection: DAB](http://images.novusbio.com/fullsize/BIRC6-Antibody-Immunohistochemistry-Paraffin-NB110-40730-img0002.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry: BIRC6 Antibody [NB110-40730] - Elevated BIRC6 expression is associated with advanced stages of prostate cancer: co-upregulation of other IAP members. Representative images of correlated expressions between BIRC6 and survivin, XIAP and cIAP1. 20x magnification, scale bar, 100 um. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/fulltext/2229), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/BIRC6-Antibody-Immunohistochemistry-NB110-40730-img0004.jpg)
![Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP1-89152] - Analysis in human cell lines A-549 and A-431 using anti-ALDH1A1 antibody. Corresponding ALDH1A1 RNA-seq data are presented for the same cell lines. Loading control: anti-PPIB.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-1-A1-ALDH1A1-Antibody-Western-Blot-NBP1-89152-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP1-89152] - Analysis in human cell lines A-549 and A-431 using anti-ALDH1A1 antibody. Corresponding ALDH1A1 RNA-seq data are presented for the same cell lines. Loading control: anti-PPIB.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP1-89152] - Staining of human cell line A549 shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-1-A1-ALDH1A1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89152-img0006.jpg)
![Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP1-89152] - Staining of human tonsil shows very weak positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-1-A1-ALDH1A1-Antibody-Immunohistochemistry-Paraffin-NBP1-89152-img0015.jpg)
![Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP1-89152] - Staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-1-A1-ALDH1A1-Antibody-Immunohistochemistry-Paraffin-NBP1-89152-img0012.jpg)
![Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP1-89152] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-1-A1-ALDH1A1-Antibody-Immunohistochemistry-Paraffin-NBP1-89152-img0013.jpg)
![Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP1-89152] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-1-A1-ALDH1A1-Antibody-Immunohistochemistry-Paraffin-NBP1-89152-img0014.jpg)
![Western Blot: HSP27 Antibody (8A7) [NB110-96431] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:8,000 of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NB110-96431) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HSP27-Antibody-8A7-Knockout-Validated-NB110-96431-img0015.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody (8A7) [NB110-96431] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:8,000 of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NB110-96431) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody (8A7) [NB110-96431] - analysis of Rat Lung tissue lysates showing detection of Hsp27 protein using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7 . Load: 15 ug protein. Block: 5% blocking solution. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/HSP27-Antibody-8A7-Western-Blot-NB110-96431-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody (8A7) [NB110-96431] - analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7 . Tissue: embryo somites. Species: Rat. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: Rhodamine-phalloidin labeled actin (red). Courtesy of: Mike Welsh, Umich.](http://images.novusbio.com/fullsize/HSP27-Antibody-8A7-Immunocytochemistry-Immunofluorescence-NB110-96431-img0007.jpg)
![Immunohistochemistry: HSP27 Antibody (8A7) [NB110-96431] - Immunohistochemistry analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7 (NB110-96431). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody (NB110-96431) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.](http://images.novusbio.com/fullsize/HSP27-Antibody-8A7-Immunohistochemistry-NB110-96431-img0016.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (8A7) [NB110-96431] - Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody at 1:5000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.](http://images.novusbio.com/fullsize/HSP27-Antibody-8A7-Immunohistochemistry-Paraffin-NB110-96431-img0008.jpg)
![Immunohistochemistry: HSP27 Antibody (8A7) [NB110-96431] - Immunohistochemistry analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7 (NB110-96431). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody (NB110-96431) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.](http://images.novusbio.com/fullsize/HSP27-Antibody-8A7-Immunohistochemistry-NB110-96431-img0017.jpg)
![Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NBP1-31258] - Paraffin-embedded endometrial, using antibody at 1:150 dilution.](http://images.novusbio.com/fullsize/GPER-GPR30-Antibody-Immunohistochemistry-Paraffin-NBP1-31258-img0009.jpg "Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NBP1-31258] - Paraffin-embedded endometrial, using antibody at 1:150 dilution.")
![Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NBP1-31258] - Paraffin-embedded endometrial, using antibody at 1:150 dilution.](http://images.novusbio.com/fullsize/GPER-GPR30-Antibody-Immunohistochemistry-Paraffin-NBP1-31258-img0008.jpg)
![Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NBP1-31258] - GPER/GPR30 antibody [C2C3], C-term detects GPER/GPR30 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human colon cancer.GPER/GPR30 stained by GPER/GPR30 antibody [C2C3], C-term (NBP1-31258) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31258_rabbit-polyclonal-gper-gpr30-antibody-91020242001028.jpg)
![Immunocytochemistry/Immunofluorescence: GPER/GPR30 Antibody [NBP1-31258] - DIV11 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GPR30 stained by GPR30 antibody [C2C3], C-term diluted at 1:100.Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [11710] diluted at 1:500.Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/antibody/nbp1-31258_rabbit-polyclonal-gper-gpr30-antibody-immunocytochemistry-immunofluorescence-16122024114732..png)
![Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NBP1-31258] - Mouse tissues.GPR30 stained by GPR30 antibody [C2C3], C-term diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/antibody/nbp1-31258_rabbit-polyclonal-gper-gpr30-antibody-immunohistochemistry-paraffin-1612202411499..png)
![<b>Orthogonal Strategies Validation. </b>Boiled and unboiled AsPC-1 whole cell and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GPER/GPR30 antibody [C2C3], C-term (NBP1-31258) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody](http://images.novusbio.com/fullsize/nbp1-31258_rabbit-polyclonal-gper-gpr30-antibody-western-blot-1411202518352213.jpg)
![<b>Orthogonal Strategies Validation. </b>Boiled and unboiled various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GPER/GPR30 antibody [C2C3], C-term (NBP1-31258) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp1-31258_rabbit-polyclonal-gper-gpr30-antibody-western-blot-1411202518344912.jpg)
![GPER/GPR30 antibody [C2C3], C-term detects GPER/GPR30 protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.Green: GPER/GPR30 stained by GPER/GPR30 antibody [C2C3], C-term (NBP1-31258) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-31258_rabbit-polyclonal-gper-gpr30-antibody-immunocytochemistry-immunofluorescence-1411202518434418.jpg)
![<b>Orthogonal Strategies Validation. </b>Non-transfected (-) and transfected (+) bolied and unboiled 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GPER/GPR30 antibody [C2C3], C-term (NBP1-31258) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp1-31258_rabbit-polyclonal-gper-gpr30-antibody-western-blot-1411202518344921.jpg)
![GPER/GPR30 antibody detects GPER/GPR30 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GPER/GPR30 stained by GPER/GPR30 antibody (NBP1-31258) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-31258_rabbit-polyclonal-gper-gpr30-antibody-immunocytochemistry-immunofluorescence-141120251841523.jpg)