Neuroscience reagents: Novus Biologicals

NBP2-01926

LIM1 Antibody (OTI2D5)

(4 Publications)

0.1 ml

NBP2-01926

Immunohistochemistry-Paraffin: LIM1 Antibody (OTI2D5) [NBP2-01926] - Staining of paraffin-embedded Human prostate tissue using anti-LIM1 mouse monoclonal antibody.

Flow Cytometry: LIM1 Antibody (OTI2D5) [NBP2-01926] - Analysis of Jurkat cells, using anti-LHX1 antibody (Red) compared to a nonspecific negative control antibody (Blue).

Immunohistochemistry-Paraffin: LIM1 Antibody (OTI2D5) [NBP2-01926] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-LIM1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: LIM1 Antibody (OTI2D5) [NBP2-01926] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-LIM1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: LIM1 Antibody (OTI2D5) [NBP2-01926] - Staining of paraffin-embedded Human Kidney tissue using anti-LIM1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: LIM1 Antibody (OTI2D5) [NBP2-01926] - Staining of paraffin-embedded Human liver tissue using anti-LIM1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: LIM1 Antibody (OTI2D5) [NBP2-01926] - Staining of paraffin-embedded Human pancreas tissue using anti-LIM1 mouse monoclonal antibody.

Flow Cytometry: LIM1 Antibody (OTI2D5) [NBP2-01926] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LIM1 antibody, and then analyzed by flow cytometry.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-71134AF350
Alexa Fluor 405	NBP2-71134AF405
Alexa Fluor 488	NBP2-71134AF488
Alexa Fluor 532	NBP2-71134AF532
Alexa Fluor 594	NBP2-71134AF594
Alexa Fluor 647	NBP2-71134AF647
Alexa Fluor 700	NBP2-71134AF700
Alexa Fluor 750	NBP2-71134AF750
Allophycocyanin	NBP2-71134APC
Biotin	NBP2-71134B
CoraFluor 1	NBP2-71134CL1
DyLight 350	NBP2-71134UV
DyLight 405	NBP2-71134V
DyLight 488	NBP2-71134G
DyLight 550	NBP2-71134R
DyLight 594	NBP2-71134DL594
DyLight 650	NBP2-71134C
DyLight 680	NBP2-71134FR
DyLight 755	NBP2-71134IR
FITC	NBP2-71134F
HRP	NBP2-71134H
Janelia Fluor 525	NBP2-71134JF525
Janelia Fluor 549	NBP2-71134JF549
Janelia Fluor 646	NBP2-71134JF646
Janelia Fluor 669	NBP2-71134JF669
PE	NBP2-71134PE
PerCP	NBP2-71134PCP
mFluor Violet 450 SE	NBP2-71134MFV450
mFluor Violet 500 SE	NBP2-71134MFV500
mFluor Violet 610 SE	NBP2-71134MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-71134

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NBP2-02577

$Immunoprecipitation: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - RASSF1A depletion does not affect nuclear F‐actin levelsWestern blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with truncated RASSF1A mutants.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRASSF1A & siRASSF1A together with XPO6 plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRNA‐resistant FLAG‐RASSF1A plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siMST2. Quantification of nuclear actin & profilin relative to Lamin A/C is shown. Error bars derive from three independent experiments & represent the SEM.Western blot analysis of exportin‐6 (XPO6) & importin‐9 (IPO9) in the absence of RASSF1A. GAPDH was used as a loading control.Confocal images of endogenous filamentous actin (F‐actin) in siCTRL & siRASSF1A cells using phalloidin staining (Alexa Fluor 568‐conjugated, red). DNA was stained with DAPI. Scale bars = 10 μm.Western blot analysis of actin, profilin, GAPDH & Lamin A/C levels in nuclear & cytoplasmic fractions of MDA‐MB‐231 cells treated with DMSO or 5′‐aza‐dC. The graph shows the nuclear levels of actin & profilin relative to Lamin A/C in MDA‐MB‐231 cells expressing RASSF1A. Error bars derive from two independent experiments & represent the SEM.Data information: Two‐tailed Student's t‐test was used for statistical analysis.*P < 0.05, **P < 0.01, ***P < 0.001.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31414556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

$Immunoprecipitation: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - RASSF1A is involved in actin & profilin nuclear export processHeLa cells treated with control or RASSF1A siRNA were fractionated into cytoplasmic & nuclear extracts. Lysates from each fraction were probed for actin & profilin alongside GAPDH (as a marker of the cytoplasmic fraction) & Lamin A/C (as a marker of the nuclear fraction). Right: quantification of nuclear actin & profilin relative to Lamin A/C is shown. Error bars derive from two independent experiments & represent the SEM.Immunofluorescence images of profilin in control & RASSF1A siRNA‐transfected HeLa cells. Right: the profilin localisation was scored as nuclear/cytoplasmic or predominantly cytoplasmic in approximately 100 cells. Error bars derive from three independent experiments & represent the SEM.Confocal images of endogenous monomeric globular actin (G‐actin) in siCTRL & siRASSF1A cells using DNase I staining (Alexa Fluor 488‐conjugated, green). Scale bars = 10 μm.Western blot analysis of actin, profilin, GAPDH & Lamin A/C levels in nuclear & cytoplasmic fractions of MDA‐MB‐231 cells treated with control pcDNA3 or RASSF1A vector. The graph shows the nuclear levels of actin & profilin relative to Lamin A/C in MDA‐MB‐231 cells expressing RASSF1A. Error bars derive from two independent experiments & represent the SEM.Data information: Two‐tailed Student's t‐test was used for statistical analysis. *P < 0.05, **P < 0.01.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31414556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

$Immunoprecipitation: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - RASSF1A depletion does not affect nuclear F‐actin levelsWestern blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with truncated RASSF1A mutants.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRASSF1A & siRASSF1A together with XPO6 plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRNA‐resistant FLAG‐RASSF1A plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siMST2. Quantification of nuclear actin & profilin relative to Lamin A/C is shown. Error bars derive from three independent experiments & represent the SEM.Western blot analysis of exportin‐6 (XPO6) & importin‐9 (IPO9) in the absence of RASSF1A. GAPDH was used as a loading control.Confocal images of endogenous filamentous actin (F‐actin) in siCTRL & siRASSF1A cells using phalloidin staining (Alexa Fluor 568‐conjugated, red). DNA was stained with DAPI. Scale bars = 10 μm.Western blot analysis of actin, profilin, GAPDH & Lamin A/C levels in nuclear & cytoplasmic fractions of MDA‐MB‐231 cells treated with DMSO or 5′‐aza‐dC. The graph shows the nuclear levels of actin & profilin relative to Lamin A/C in MDA‐MB‐231 cells expressing RASSF1A. Error bars derive from two independent experiments & represent the SEM.Data information: Two‐tailed Student's t‐test was used for statistical analysis.*P < 0.05, **P < 0.01, ***P < 0.001.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31414556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Reactivity:

Hu, Mu, Rt, Ca, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Ca, Pm

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-73605AF350
Alexa Fluor 405	NBP2-73605AF405
Alexa Fluor 488	NBP2-73605AF488
Alexa Fluor 532	NBP2-73605AF532
Alexa Fluor 594	NBP2-73605AF594
Alexa Fluor 647	NBP2-73605AF647
Alexa Fluor 700	NBP2-73605AF700
Alexa Fluor 750	NBP2-73605AF750
Allophycocyanin	NBP2-73605APC
Biotin	NBP2-73605B
CoraFluor 1	NBP2-73605CL1
DyLight 350	NBP2-73605UV
DyLight 405	NBP2-73605V
DyLight 488	NBP2-73605G
DyLight 550	NBP2-73605R
DyLight 594	NBP2-73605DL594
DyLight 650	NBP2-73605C
DyLight 680	NBP2-73605FR
DyLight 755	NBP2-73605IR
FITC	NBP2-73605F
HRP	NBP2-73605H
Janelia Fluor 525	NBP2-73605JF525
Janelia Fluor 549	NBP2-73605JF549
Janelia Fluor 646	NBP2-73605JF646
Janelia Fluor 669	NBP2-73605JF669
PE	NBP2-73605PE
PerCP	NBP2-73605PCP
mFluor Violet 450 SE	NBP2-73605MFV450
mFluor Violet 500 SE	NBP2-73605MFV500
mFluor Violet 610 SE	NBP2-73605MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-73605

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NBP1-47923

OCT4 Antibody (OTI9B7)

(6 Publications)

0.1 ml

NBP1-47923

Immunohistochemistry-Paraffin: OCT4 Antibody (OTI9B7) [NBP1-47923] - Staining of paraffin-embedded Human Ovary tissue using anti-41186 mouse monoclonal antibody.

Immunocytochemistry/Immunofluorescence: OCT4 Antibody (OTI9B7) [NBP1-47923] - Representative IF IHC-stained sections of MDBMSCC. Expression of both SOX2 (red) and OCT4 (green), appearing as orange, was seen on cells within the tumor nests and the stroma, and the endothelium of the microvessels within the stroma. Scale bars: 20 um. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fsurg.2016.00046), licensed under a CC-BY license.

Immunohistochemistry-Paraffin: OCT4 Antibody (OTI9B7) [NBP1-47923] - Staining of paraffin-embedded Human endometrium tissue using anti-41186 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: OCT4 Antibody (OTI9B7) [NBP1-47923] - Staining of paraffin-embedded Human lung tissue using anti-41186 mouse monoclonal antibody.

Immunohistochemistry: OCT4 Antibody (OTI9B7) [NBP1-47923] - Representative DAB IHC-stained sections of MDBMSCC demonstrating nuclear expression of EMA of cells within the tumor nests [(A), brown]. Expression of SOX2 was seen in cells within the tumor nests [(B), brown, thick arrows] & the stroma [(B), brown, arrowheads], & on the endothelium of the microvessels within the stroma [(B), brown, thin arrows]. Expression of SALL4 was limited to cells within the tumor nests [(C), brown, thick arrows] & the endothelium of the microvessels [(C), brown, thin arrows]. OCT4 was also expressed in cells within tumor nests [(D), brown, thick arrows] & the stroma [(D), brown, arrowheads], & the endothelium of the microvessels within the stroma [(D), brown, thin arrows]. Expression of pSTAT3 was detected on cells within the tumor nests [(E), brown, thick arrows] & the stroma [(E), brown, arrowheads], & the endothelium of the microvessels within the stroma [(E), brown, thin arrows]. NANOG was also seen in cells within the tumor nests [(F), brown, thick arrows] & the stroma [(F), brown, arrowheads], & the endothelium of the microvessels within the stroma [(F), brown, thin arrows]. CD44 expression was seen as membranous staining of the tumor nest cells [(G), brown]. Original magnification: 400×. Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/Article/10.3389/fsurg.2016.00046/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Representative IF IHC-stained sections of MDBMSCC demonstrating the expression of pSTAT3 [(A), red] and EMA [(A), green] by cells within the tumor nests. There was a CSC subpopulation remonstrating nuclear co-expression of STAT3 [(B), red] and CD34 [(B), green], appearing as orange, on the endothelium of the microvessels within the stroma; and another subpopulation staining only positively for pSTAT3 within the stroma [(B), red]. Nuclear expression of NANOG [(C), red] was demonstrated on the endothelium of the microvessels which expressed CD34 [(C), green] within stroma. The NANOG+ cells [(C), red] that do not express CD34 were seen within the tumor nests and the stroma. SOX2 [(D), red] was also expressed by cells within tumor nests and the stroma, and the endothelium of the microvessels expressing CD34 [(D), green]. Nuclear expression of both SOX2 [(E), red] and SALL4 [(E), green], appearing as orange, was seen on the cells within the tumor nests and the stroma. Expression of both SOX2 [(F), red] and OCT4 [(F), green], appearing as orange, was seen on cells within the tumor nests and the stroma, and the endothelium of the microvessels within the stroma. pSTAT3 [(G), red] and membranous staining CD44 [(G), green] were co-expressed by cells within the tumor nests. Scale bars: 20 μm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/27532037), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-71225AF350
Alexa Fluor 405	NBP2-71225AF405
Alexa Fluor 488	NBP2-71225AF488
Alexa Fluor 532	NBP2-71225AF532
Alexa Fluor 594	NBP2-71225AF594
Alexa Fluor 647	NBP2-71225AF647
Alexa Fluor 700	NBP2-71225AF700
Alexa Fluor 750	NBP2-71225AF750
Allophycocyanin	NBP2-71225APC
Biotin	NBP2-71225B
CoraFluor 1	NBP2-71225CL1
DyLight 350	NBP2-71225UV
DyLight 405	NBP2-71225V
DyLight 488	NBP2-71225G
DyLight 550	NBP2-71225R
DyLight 594	NBP2-71225DL594
DyLight 650	NBP2-71225C
DyLight 680	NBP2-71225FR
DyLight 755	NBP2-71225IR
FITC	NBP2-71225F
HRP	NBP2-71225H
Janelia Fluor 525	NBP2-71225JF525
Janelia Fluor 549	NBP2-71225JF549
Janelia Fluor 646	NBP2-71225JF646
Janelia Fluor 669	NBP2-71225JF669
PE	NBP2-71225PE
PerCP	NBP2-71225PCP
mFluor Violet 450 SE	NBP2-71225MFV450
mFluor Violet 500 SE	NBP2-71225MFV500
mFluor Violet 610 SE	NBP2-71225MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-71225

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NB100-53791

CPT1A Antibody

(9 Publications)

0.1 mg

NB100-53791

Immunohistochemistry-Paraffin: CPT1A Antibody [NB100-53791] - Negative Control showing staining of paraffin embedded Human Liver, with no primary antibody.

Immunohistochemistry-Paraffin: CPT1A Antibody [NB100-53791] - (5ug/ml) staining of paraffin embedded Human Liver. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Unconjugated

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NBP1-97712

Cytokeratin 19 Antibody (RCK108) - BSA Free

(3 Publications)

0.1 mg

NBP1-97712

Flow Cytometry: Cytokeratin 19 Antibody (RCK108) [NBP1-97712] - Flow Cytometry: Cytokeratin 19 Antibody (RCK108) [DyLight 650] [NBP1-97712C] - An intracellular stain was performed on HT-29 cells with Cytokeratin 19 (RCK108) NBP1-97712C (blue) and a matched isotype control NBP1-97005C (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight650. Image using the DyLight 650 form of this antibody.

Immunohistochemistry-Frozen: Cytokeratin 19 Antibody (RCK108) [NBP1-97712] - Analysis using the Unpurified version of NBP1-97712. Immunohistochemistry on frozen section of human liver bile duct epithelium

Immunohistochemistry-Frozen: Cytokeratin 19 Antibody (RCK108) [NBP1-97712] - Immunohistochemistry on a frozen tissue sections of human kidney with NBP1-97712 (RCK108; dilution 1:500).

Immunohistochemistry-Frozen: Cytokeratin 19 Antibody (RCK108) [NBP1-97712] - Immunohistochemistry on frozen tissue sections of human colon with NBP1-97712 (RCK108; dilution 1:500).

Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (RCK108) [NBP1-97712] - Indirect immunoperoxidase staining of human small intestine paraffin tissue section with NBP1-97712 (RCK108; Mouse anti Cytokeratin 19). Dilution 1:50 and microwave pretreatment. Specific staining of the epithelial cells. No reactivity in the connective tissues.

Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (RCK108) [NBP1-97712] - Indirect immunoperoxidase staining of human placenta paraffin tissue section with NBP1-97712 (RCK108; Mouse anti Cytokeratin 19). Dilution 1:50 and microwave pretreatment. Specific staining of the epithelial cells. No reactivity in the connective tissues.

Reactivity:

Hu, Rt, Ze

Species Glossary

Reactivity: Hu, Rt, Ze

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-97712AF350
Alexa Fluor 405	NBP1-97712AF405
Alexa Fluor 488	NBP1-97712AF488
Alexa Fluor 532	NBP1-97712AF532
Alexa Fluor 594	NBP1-97712AF594
Alexa Fluor 647	NBP1-97712AF647
Alexa Fluor 700	NBP1-97712AF700
Alexa Fluor 750	NBP1-97712AF750
Allophycocyanin	NBP1-97712APC
Biotin	NBP1-97712B
CoraFluor 1	NBP1-97712CL1
DyLight 350	NBP1-97712UV
DyLight 405	NBP1-97712V
DyLight 488	NBP1-97712G
DyLight 550	NBP1-97712R
DyLight 594	NBP1-97712DL594
DyLight 650	NBP1-97712C
DyLight 680	NBP1-97712FR
DyLight 755	NBP1-97712IR
FITC	NBP1-97712F
HRP	NBP1-97712H
Janelia Fluor 525	NBP1-97712JF525
Janelia Fluor 549	NBP1-97712JF549
Janelia Fluor 646	NBP1-97712JF646
Janelia Fluor 669	NBP1-97712JF669
PE	NBP1-97712PE
PerCP	NBP1-97712PCP
mFluor Violet 450 SE	NBP1-97712MFV450
mFluor Violet 500 SE	NBP1-97712MFV500
mFluor Violet 610 SE	NBP1-97712MFV610

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NBP2-22116

Cytokeratin 19 Antibody (4E8) - BSA Free

(2 Publications)

0.1 ml

NBP2-22116

Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (4E8) [NBP2-22116] - Immunohistochemical analysis of paraffin-embedded human stomach cancer tissues using Cytokeratin 19 mouse mAb with DAB staining.

Western Blot: Cytokeratin 19 Antibody (4E8) [NBP2-22116] - Western blot analysis using Cytokeratin 19 mAb against human Cytokeratin 19 (AA: 115-269) recombinant protein. (Expected MW is 43.1 kDa)

Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (4E8) [NBP2-22116] - Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using Cytokeratin 19 mouse mAb with DAB staining.

Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (4E8) [NBP2-22116] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using Cytokeratin 19 mouse mAb with DAB staining.

ELISA: Cytokeratin 19 Antibody (4E8) [NBP2-22116] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-22116AF350
Alexa Fluor 405	NBP2-22116AF405
Alexa Fluor 488	NBP2-22116AF488
Alexa Fluor 532	NBP2-22116AF532
Alexa Fluor 594	NBP2-22116AF594
Alexa Fluor 647	NBP2-22116AF647
Alexa Fluor 700	NBP2-22116AF700
Alexa Fluor 750	NBP2-22116AF750
Allophycocyanin	NBP2-22116APC
Biotin	NBP2-22116B
CoraFluor 1	NBP2-22116CL1
DyLight 350	NBP2-22116UV
DyLight 405	NBP2-22116V
DyLight 488	NBP2-22116G
DyLight 550	NBP2-22116R
DyLight 594	NBP2-22116DL594
DyLight 650	NBP2-22116C
DyLight 680	NBP2-22116FR
DyLight 755	NBP2-22116IR
FITC	NBP2-22116F
HRP	NBP2-22116H
Janelia Fluor 525	NBP2-22116JF525
Janelia Fluor 549	NBP2-22116JF549
Janelia Fluor 646	NBP2-22116JF646
Janelia Fluor 669	NBP2-22116JF669
PE	NBP2-22116PE
PerCP	NBP2-22116PCP
mFluor Violet 450 SE	NBP2-22116MFV450
mFluor Violet 500 SE	NBP2-22116MFV500
mFluor Violet 610 SE	NBP2-22116MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-22116SS
Azide and BSA Free	NBP2-80689

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NBP2-60735

LC3B Antibody (1251B) - BSA Free

0.1 mg

NBP2-60735

Immunocytochemistry/Immunofluorescence: LC3B Antibody (1251B) [NBP2-60735] - HeLa cells were treated with 50uM CQ (Chloroquine) overnight, fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti- [Catalog # NBP2-60735] at 2 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

<b>Biological Strategies Validation. </b>Western Blot: LC3B Antibody (1251B) [NBP2-60735] - Total protein from HeLa and Neuro2A cells treated with or without 50 uM chloroquine (CQ) for 24 hours was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF (Polyvinylidene difluoride) membrane and blocked in 5% non-fat milk in TBST (Tris-buffered saline, 0.1% Tween 20). The membrane was probed with 12.0 ug/ml anti-LC3B Antibody (1251B) [Catalog # NBP2-60735] in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Immunohistochemistry-Paraffin: LC3B Antibody (1251B) [NBP2-60735] - Analysis of a FFPE (Formalin-Fixed Paraffin-Embedded) tissue section of mouse brain using anti-LC3B antibody (clone 1251B) [Catalog # NBP2-60735] at 5ug/ml concentration (1:200 dilution). The primary antibody binding to LC3 in cells was detected using HRP conjugated anti-Rabbit secondary antibody with DAB (3,3'Diaminobenzidine) reagent, and the sections were further counterstained with hematoxylin for labeling cellular nuclei. This LC3 antibody generated a diffused staining in all cell types (except the endothelial cells of blood vessels) and the signal was strongest in a subset of neuronal cells. A few cells depicted LC3 puncta formation/dotted staining in apparently autophagic cells.

<b>Biological Strategies Validation. </b>Flow (Intracellular): LC3B Antibody (1251B) [NBP2-60735] - HeLa cells were either (A) untreated or (B) treated with 50uM chloroquine for 24 hours. An intracellular stain was performed with anti-LC3B (1251B) antibody [Catalog # NBP2-60735] (blue) and a matched isotype control [Catalog # MAB1050] (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by rabbit IgG APC-conjugated secondary antibody (F0111, R&D Systems).

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-60735AF350
Alexa Fluor 405	NBP2-60735AF405
Alexa Fluor 488	NBP2-60735AF488
Alexa Fluor 532	NBP2-60735AF532
Alexa Fluor 594	NBP2-60735AF594
Alexa Fluor 647	NBP2-60735AF647
Alexa Fluor 700	NBP2-60735AF700
Alexa Fluor 750	NBP2-60735AF750
Allophycocyanin	NBP2-60735APC
Biotin	NBP2-60735B
CoraFluor 1	NBP2-60735CL1
DyLight 350	NBP2-60735UV
DyLight 405	NBP2-60735V
DyLight 488	NBP2-60735G
DyLight 550	NBP2-60735R
DyLight 594	NBP2-60735DL594
DyLight 650	NBP2-60735C
DyLight 680	NBP2-60735FR
DyLight 755	NBP2-60735IR
FITC	NBP2-60735F
HRP	NBP2-60735H
Janelia Fluor 525	NBP2-60735JF525
Janelia Fluor 549	NBP2-60735JF549
Janelia Fluor 646	NBP2-60735JF646
Janelia Fluor 669	NBP2-60735JF669
PE	NBP2-60735PE
PerCP	NBP2-60735PCP
mFluor Violet 450 SE	NBP2-60735MFV450
mFluor Violet 500 SE	NBP2-60735MFV500
mFluor Violet 610 SE	NBP2-60735MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-60735SS
Azide and BSA Free	NBP2-80831

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NBP1-89468

MEF2C Antibody - BSA Free

(4 Publications)

0.1 ml

NBP1-89468

Staining of human liver shows no positivity in hepatocytes as expected.

Staining of human cerebral cortex shows strong nuclear positivity in neurons.

Staining of human skeletal muscle shows moderate nuclear positivity in myocytes.

Staining of human tonsil shows moderate nuclear positivity in germinal center cells.

Analysis in human cerebral cortex and liver tissues using NBP1-89468 antibody. Corresponding MEF2C RNA-seq data are presented for the same tissues.

ChIP-Exo-Seq composite graph for Anti-MEF2C (NBP1-89468) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-89468-25ul

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NBP1-90186

SOD1/Cu-Zn SOD Antibody - BSA Free

(3 Publications)

0.1 ml

NBP1-90186

Immunohistochemistry-Paraffin: SOD1/Cu-Zn SOD Antibody [NBP1-90186] - staining of human liver shows strong nuclear and cytoplasmic positivity in hepatocytes.

Immunohistochemistry-Paraffin: SOD1/Cu-Zn SOD Antibody [NBP1-90186] - Staining of human pancreas shows very weak nuclear positivity in exocrine glandular cells.

Immunohistochemistry-Paraffin: SOD1/Cu-Zn SOD Antibody [NBP1-90186] - Staining of human testis shows moderate nuclear and cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.

Immunohistochemistry-Paraffin: SOD1/Cu-Zn SOD Antibody [NBP1-90186] - Staining of human fallopian tube shows moderate nuclear positivity in glandular cells.

Simple Western: SOD1/Cu-Zn SOD Antibody [NBP1-90186] - Simple Western lane view shows a specific band for SOD1 in 0.2 mg/ml of RT-4 (left) and U-251MG sp (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: SOD1/Cu-Zn SOD Antibody [NBP1-90186] - Electropherogram image(s) of corresponding Simple Western lane view. SOD1/Cu-Zn SOD antibody was used at 1:30 dilution on RT-4 and U-251MG sp lysates(s).

<b>Genetic Strategies Validation. </b>Western Blot: SOD1/Cu-Zn SOD Antibody [NBP1-90186] - Analysis in Hep-G2 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-SOD1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-90186-25ul

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NBP2-42388

LAMC2 Antibody (CL2980) - BSA Free

(4 Publications)

0.1 ml

NBP2-42388

Immunocytochemistry/Immunofluorescence: LAMC2 Antibody (CL2980) [NBP2-42388] - Staining of A-431 cells using the Anti-LAMC2 monoclonal antibody, showing specific staining in the cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Western Blot: LAMC2 Antibody (CL2980) [NBP2-42388] - Analysis of purified human recombinant Laminin-332, Laminin-421, Laminin-511, Laminin-121 and Laminin-221.

Immunohistochemistry-Paraffin: LAMC2 Antibody (CL2980) [NBP2-42388] - Staining of human skin shows moderate positivity in basement membrane of epidermis.

Immunohistochemistry-Paraffin: LAMC2 Antibody (CL2980) [NBP2-42388] - Staining of human fallopian tube shows moderate positivity in basement membrane of glandular cells.

Immunohistochemistry-Paraffin: LAMC2 Antibody (CL2980) [NBP2-42388] - Staining of human small intestine shows moderate positivity in basement membrane of glandular cells.

Immunohistochemistry-Paraffin: LAMC2 Antibody (CL2980) [NBP2-42388] - Staining of human liver shows no positivity in hepatocytes as expected.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-44421AF350
Alexa Fluor 405	NBP3-44421AF405
Alexa Fluor 488	NBP3-44421AF488
Alexa Fluor 532	NBP3-44421AF532
Alexa Fluor 594	NBP3-44421AF594
Alexa Fluor 647	NBP3-44421AF647
Alexa Fluor 700	NBP3-44421AF700
Alexa Fluor 750	NBP3-44421AF750
Allophycocyanin	NBP3-44421APC
Biotin	NBP3-44421B
CoraFluor 1	NBP3-44421CL1
DyLight 350	NBP3-44421UV
DyLight 405	NBP3-44421V
DyLight 488	NBP3-44421G
DyLight 550	NBP3-44421R
DyLight 594	NBP3-44421DL594
DyLight 650	NBP3-44421C
DyLight 680	NBP3-44421FR
DyLight 755	NBP3-44421IR
FITC	NBP3-44421F
HRP	NBP3-44421H
Janelia Fluor 525	NBP3-44421JF525
Janelia Fluor 549	NBP3-44421JF549
Janelia Fluor 646	NBP3-44421JF646
Janelia Fluor 669	NBP3-44421JF669
PE	NBP3-44421PE
PerCP	NBP3-44421PCP
mFluor Violet 450 SE	NBP3-44421MFV450
mFluor Violet 500 SE	NBP3-44421MFV500
mFluor Violet 610 SE	NBP3-44421MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-44421
BSA Free	NBP2-42388-25ul

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NBP1-87493

Monoamine Oxidase B Antibody - BSA Free

(5 Publications)

0.1 ml

NBP1-87493

Western Blot: Monoamine Oxidase B Antibody [NBP1-87493] - Analysis in human liver tissue.

Immunohistochemistry-Paraffin: Monoamine Oxidase B Antibody [NBP1-87493] - Staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: Monoamine Oxidase B Antibody [NBP1-87493] - Staining of human liver shows moderate to strong granular cytoplasmic positivity in hepatocytes.

Immunohistochemistry-Paraffin: Monoamine Oxidase B Antibody [NBP1-87493] - Staining of human testis shows moderate to strong granular cytoplasmic positivity in Leydig cells.

Glutamate-driven astrocytic MAO-B leading to reactive astrogliosis and astrocytic scar formation. a Schematic representation of glutamate-driven astrocytic MAO-B inducing reactive astrogliosis and astrocytic scar formation. b-d Western blot analysis for EAAT1 and MAO-B expression in astrocyte monocultures upon glutamate treatment. Glutamate treatment increased MAO-B expression in astrocytes. Blockage of glutamate transport activity by using glutamate transporter inhibitor (TBOA) decreased MAO-B expression. e, f Reactivity of astrocyte monocultures upon glutamate treatment assessed by immunostaining with GFAP. Inhibiting glutamate transport (TBOA) or MAO-B (KDS2010) decreased GFAP expression. g ELISA for CSPGs deposited from reactive astrocytes. h, i Immunofluorescence images and quantification of GFAP intensity in the mouse tissues. j, k Immunofluorescence images and quantification of CSPGs intensity in the mouse tissues. Quantitative data were presented as means +/- SD (n = 3, unless otherwise noted). *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001. p values were calculated by two-tailed unpaired Student's t-test for comparisons between two groups, and by one-way ANOVA for multiple comparisons. Scale bars represent 100 μm Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37468961), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

MAO-B-positive mitovesicles are more numerous in the brain than MAO-A-positive mitovesicles. (A, B) Representative flow cytometry analyses of Fr8 brain EVs (mitovesicle-enriched, second columns) immunolabeled with antibodies against MAO-A (A) and MAO-B (B). The left columns show the same analyses performed on a 1:1:1 v/v mixture of Fr1, Fr2, and Fr3 EVs (microvesicle-enriched) isolated from the same brains (negative control). The right columns show mitovesicles incubated with the secondary antibody only. The gating strategy is shown for all conditions (first rows). Both the y- and the x-axis are in a log10 scale. 20,000 EVs were acquired for each experiment. FSC-A: forward scatter– area. SSC-A: side scatter– area. Ab: antibody. (C) Percentage of mitovesicles positive for either MAO-A or MAO-B. n = 3 mice per group. The percentage of positive EVs was determined on the basis of the secondary antibody control gate of each sample (set to 0.8% maximum). Bars: mean +/- SEM. Statistical test used: two-tailed, unpaired Student’s t-test. MAO-A vs. MAO-B, P = 0.0004. *** P < 0.001 Image collected and cropped by CiteAb from the following open publication (//molecularneurodegeneration.biomedcentral.com/articles/10.1186/s13024-024-00721-z), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP1-89370

S100A10 Antibody - BSA Free

(4 Publications)

0.1 ml

NBP1-89370

Staining of human lung shows strong membranous positivity in macrophages.

<b>Genetic Strategies Validation. </b>Analysis in human lung and skeletal muscle tissues using NBP1-89370 antibody. Corresponding S100A10 RNA-seq data are presented for the same tissues.

Staining of human small intestine shows strong membranous positivity in glandular cells.

Staining of human skin shows strong membranous positivity in squamous epithelial cells.

<b>Orthogonal Strategies Validation. </b>Analysis in human cell lines Caco-2 and HEK293 using Anti-S100A10 antibody. Corresponding S100A10 RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.

<b>Genetic Strategies Validation. </b>Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-S100A10 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

The AT2-blocker PD123319 induces S100A10 expression in astrocytes with increased proliferation whereas Telmisartan does not affect S100A10 or Ki67 expression. *p < 0.05, **p < 0.01, and ***p < 0.001 compared different experimental groups as marked by horizontal bar; graphs depict mean values +/- standard error of the mean (SEM). A Experimental timeline. Three to five days after subculturing, astrocytes were left unstimulated or treated with 10 uM telmisartan or 10 uM PD123319 for 48 h. They were then exposed to 2 h of oxygen–glucose deprivation (OGD) before they were allowed to recover in regular culture medium for 24 h. Afterward, the cells were used for further experiments. Unstimulated astrocytes, which were exposed to 2 h of OGD, served as controls. B S100A10 marker expression in untreated (unstimulated) or OGD-treated (2 h of OGD) astrocytes with or without the additional preincubation with 10 uM telmisartan or 10 uM PD123319. C Proliferation rate of untreated (unstimulated) and OGD-treated (2 h OGD) astrocytes, with or without preincubation with 10 uM telmisartan or 10 uM PD123319 over 48 h as assessed by mRNA-ki67 expression. D ATP concentration of untreated or OGD-treated astrocytes with or without preincubation with 10 uM telmisartan or 10 uM PD123319 over 48 h. E Expression of the connexin Cx43 in untreated or OGD-treated astrocytes with or without preincubation with 10 uM telmisartan and 10 uM PD123319 over 48 h. F Representative immunocytochemical stainings of astrocytes with Glia Fibrillary Acidic Protein (GFAP) + (green) and S100A10 + (red) in unstimulated (control), PD123319-preincubated (PD123319), or after 2 h of OGD (OGD + Control). Hoechst stained all cell nuclei blue; scale bars = 50 um Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/38926677), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-89370-25ul

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Marker

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Kit Type

Lysate Type

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

LIM1 Antibody (OTI2D5)

Profilin 1 Antibody (OTI1D5)

OCT4 Antibody (OTI9B7)

CPT1A Antibody

Cytokeratin 19 Antibody (RCK108) - BSA Free

Cytokeratin 19 Antibody (4E8) - BSA Free

LC3B Antibody (1251B) - BSA Free

MEF2C Antibody - BSA Free

SOD1/Cu-Zn SOD Antibody - BSA Free

LAMC2 Antibody (CL2980) - BSA Free

Monoamine Oxidase B Antibody - BSA Free

S100A10 Antibody - BSA Free