| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05194-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-157SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-27395 | |||
| BSA Free | NBP2-27396SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33132 | |||
| IHC-Prediluted | NBP2-44711 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22116SS | |||
| Azide and BSA Free | NBP2-80689 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-65284SS | |||
| Azide and BSA Free | NBP2-80625 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89065-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50057SS | |||
![Western Blot: NF-L Antibody [NB300-131] - Analysis of different tissue lysates using rabbit pAb to NF-L, NB300-131, dilution 1:20,000. in green. [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. The strong band at 68kDa corresponds to the NF-L protein.](http://images.novusbio.com/fullsize/NF-L-Antibody-Western-Blot-NB300-131-img0004.jpg "Western Blot: NF-L Antibody [NB300-131] - Analysis of different tissue lysates using rabbit pAb to NF-L, NB300-131, dilution 1:20,000. in green. [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. The strong band at 68kDa corresponds to the NF-L protein.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: NF-L Antibody [NB300-131] - Neurofilament (NF-L antibody) used as axon marker in WT and KO mice using immunofluorescence staining to illustrate the axon morphology of retinal ganglion cells. Central part of retina is enlarged and displayed in WT and KO mice. Scale bars are 500 micrometers for left and 200 micrometers for right. Citation: Lin Y-S, Kuo K-T, Chen S-K, Huang H-S (2018) RBFOX3/NeuN is dispensable for visual function. PLoS ONE 13(2): e0192355. //doi.org/10.1371/journal.pone.0192355](http://images.novusbio.com/fullsize/NF-L-Antibody-Immunofluorescence-NB300-131-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: 68kDa Neurofilament Light Antibody [NB300-131] - Mixed neuron/glia cultures from newborn rat brain stained with NB300-220 antibody to peripherin (green) and rabbit polyclonal antibody to NF-L RPCA-NF-L (red channel). A class of large neurons, like the one in the middle of this image, contain peripherin, while the majority of neurons and their processes contain NF-L and not peripherin. Interestingly, the periperin positive cells often contain a cytoplasmic inclusion next to the nucleus which stains for both peripherin and NF-L, and so appears golden in this kind of image. The blue channel reveals the localization of DNA](http://images.novusbio.com/fullsize/68kDa-Neurofilament-Light-Antibody-Immunocytochemistry-Immunofluorescence-NB300-131-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: NF-L Antibody [NB300-131] - Analysis of mouse cerebellum section stained with rabbit pAb to NF-L, NB300-131, dilution 1:5,000 in red, and costained with chicken pAb to MBP, dilution 1:5,000, in green. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. NB300-131 antibody labels dendrites and axons of neuronal cells, and MBP antibody stains the network of myelin sheathes around axons.](http://images.novusbio.com/fullsize/NF-L-Antibody-Immunocytochemistry-Immunofluorescence-NB300-131-img0003.jpg)
![Immunohistochemistry Free-Floating: alpha-Synuclein Antibody (2A7) [NBP1-05194] - Analysis of rat cerebellum section costained with mouse mAb to alpha-synuclein NBP1-05194, dilution 1:1,000, in red, and rabbit pAb to GFAP, dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The alpha-synuclein protein is concentrated in synaptic regions, while the GFAP antibody stains the filamentous cytoskeleton of Bergmann glia and astrocytic cells.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-2A7-Immunocytochemistry-Immunofluorescence-NBP1-05194-img0004.jpg "Immunohistochemistry Free-Floating: alpha-Synuclein Antibody (2A7) [NBP1-05194] - Analysis of rat cerebellum section costained with mouse mAb to alpha-synuclein NBP1-05194, dilution 1:1,000, in red, and rabbit pAb to GFAP, dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The alpha-synuclein protein is concentrated in synaptic regions, while the GFAP antibody stains the filamentous cytoskeleton of Bergmann glia and astrocytic cells.")
![Simple Western: alpha-Synuclein Antibody (2A7) [NBP1-05194] - Synuclein-alpha Antibody (2A7) [NBP1-05194] - Simple Western lane view shows a specific band for alpha-Synuclein in 0.05 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Synuclein-alpha-Antibody-2A7-Simple-Western-NBP1-05194-img0003.jpg)
![Western Blot: alpha-Synuclein Antibody (2A7) [NBP1-05194] - Western blot analysis of tissue lysates using mAb to alpha-synuclein NBP1-05194. dilution 1:1,000 in green. Lane [1] protein standard in red, [2] whole rat brain lysate, [3] rat spinal cord lysate. Strong band at about 15kDa corresponds to alpha-synuclein protein.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-2A7-Western-Blot-NBP1-05194-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Synuclein-alpha Antibody (2A7) [NBP1-05194] - Mixed neuron-glial cultures stained with NBP1-05194, our monoclonal antibody to alpha-synuclein (red) and chicken polylclonal antibody to MAP2 NB300-213 (green). The alpha-synuclein antibody stains vesicular structures the perikarya and processes of the neurons in this image. Note that some of the neuronal perikarya contain much more alpha-synuclein than others. The blue channel shows the localization of DNA.](http://images.novusbio.com/fullsize/Synuclein-alpha-Antibody-2A7-Immunocytochemistry-Immunofluorescence-NBP1-05194-img0002.jpg)
![Flow Cytometry: alpha-Synuclein Antibody (2A7) [NBP1-05194] - An intracellular stain was performed on SK-MEL-28 cells with alpha-Synuclein [2A7] Antibody NBP1-05194AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-2A7-Flow-Cytometry-NBP1-05194-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Somatostatin R2/SSTR2 Antibody [NB300-157] - U2OS cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with antibody at 1:50 overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at 1:500. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Somatostatin-R2-SSTR2-Antibody-Immunocytochemistry-Immunofluorescence-NB300-157-img0005.jpg "Immunocytochemistry/Immunofluorescence: Somatostatin R2/SSTR2 Antibody [NB300-157] - U2OS cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with antibody at 1:50 overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at 1:500. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Immunohistochemistry-Paraffin: Somatostatin R2/SSTR2 Antibody [NB300-157] - Staining of Somatostatin Receptor 2 in mouse pancreas using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Somatostatin-Receptor-2-Antibody-Immunohistochemistry-Paraffin-NB300-157-img0004.jpg)
![Immunohistochemistry-Paraffin: Somatostatin R2/SSTR2 Antibody [NB300-157] - Staining of paraffin embedded rat pancreas tissue.](http://images.novusbio.com/fullsize/Somatostatin-Receptor-2-Antibody-Immunohistochemistry-Paraffin-NB300-157-img0003.jpg)
![Western Blot: HMGB1/HMG-1 Antibody (19N10B7) [NBP2-27396] - Hepatocyte protein lysate at 1:1000 4C overnight. Image from verified customer review.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-19N10B7-Western-Blot-NBP2-27396-img0034.jpg "Western Blot: HMGB1/HMG-1 Antibody (19N10B7) [NBP2-27396] - Hepatocyte protein lysate at 1:1000 4C overnight. Image from verified customer review.")
![Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) [NBP2-27396] - Formalin-fixed, paraffin-embedded human liver tissue stained with HMGB1 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-19N10B7-Immunohistochemistry-Paraffin-NBP2-27396-img0035.jpg)
![Flow Cytometry: HMGB1/HMG-1 Antibody (19N10B7) [NBP2-27396] - Intracellular analysis using HMGB1 antibody. Human Jurkat cells were probed using 0.5 ug of HMGB1 antibody (red) and 0.5 ug of isotype control antibody (green).](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-19N10B7-Flow-Cytometry-NBP2-27396-img0021.jpg)
![Western Blot: HMGB1/HMG-1 Antibody (19N10B7) [NBP2-27396] - Analysis using 2 ug/ml concentration of HMGB1 antibody (clone 19N10B7) on (A) Full-length human HMGB1 protein, (B) Human Jurkat cell lysate and (C) Mouse NIH 3T3 cell lysate. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution was used for this test.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-19N10B7-Western-Blot-NBP2-27396-img0033.jpg)
![Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) [NBP2-27396] - Formalin-fixed, paraffin-embedded human spleen tissue stained with HMGB1 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-19N10B7-Immunohistochemistry-Paraffin-NBP2-27396-img0022.jpg)
![Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) [NBP2-27396] - Formalin-fixed, paraffin-embedded human breast stained with HMGB1 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-19N10B7-Immunohistochemistry-Paraffin-NBP2-27396-img0023.jpg)
![Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) [NBP2-27396] - Formalin-fixed paraffin-embedded human prostate tissue stained with HMGB1 antibody at 10 ug/ml concentration. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-19N10B7-Immunohistochemistry-Paraffin-NBP2-27396-img0024.jpg)
![Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) [NBP2-27396] - Analysis of formalin-fixed, decalcified, paraffin embedded tissue section from the paws of mouse (collagen-induced arthritis model) using HMGB1 antibody (clone 19N10B7) at 5 ug/ml concentration. [Image courtesy of Dr Ulf Andersson, Karolinska Institute].](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-19N10B7-Immunohistochemistry-Paraffin-NBP2-27396-img0025.jpg)
![Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) [NBP2-27396] - IHC-P detection of HMGB1 protein in a formalin-fixed paraffin-embedded tissue section of human stomach using HMGB1 antibody (clone 19N10B7) at 5 ug/ml concentration. Distinct nuclear staining of HMGB1 was observed in different cells of the glandular stomach.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-19N10B7-Immunohistochemistry-Paraffin-NBP2-27396-img0026.jpg)
![Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) [NBP2-27396] - IHC-P detection of HMGB1 protein in a formalin-fixed paraffin-embedded tissue section of normal human skin using HMGB1 antibody (clone 19N10B7) at 5 ug/ml concentration. The various cells of the epidermal layer showed intense nuclear staining along with weak cytoplasmic staining. The blood vessels, glandular cells and the other cells in dermal layer also showed nuclear positivity for HMGB1 immunostaining.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-19N10B7-Immunohistochemistry-Paraffin-NBP2-27396-img0027.jpg)
![Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) [NBP2-27396] - IHC-P detection of HMGB1 protein in a formalin-fixed paraffin-embedded tissue section of normal human brain using HMGB1 antibody (clone 19N10B7) at 5 ug/ml concentration. Representative image shows a distinct nuclear immunostaining of HMGB1 in the various brain cells.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-19N10B7-Immunohistochemistry-Paraffin-NBP2-27396-img0028.jpg)
![Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) [NBP2-27396] - IHC-P detection of HMGB1 protein in a formalin-fixed paraffin-embedded tissue section of normal human colon using HMGB1 antibody (clone 19N10B7) at 5 ug/ml concentration. Representative image shows specific HMGB1 nuclear positivity in different mucosal cells of the colon.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-19N10B7-Immunohistochemistry-Paraffin-NBP2-27396-img0029.jpg)
![Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) [NBP2-27396] - IHC-P detection of HMGB1 protein in a formalin-fixed paraffin-embedded tissue section of malignant stromal tumor of small bowel from human using HMGB1 antibody (clone 19N10B7) at 5 ug/ml concentration. HMGB1 immunopositivity of differential intensity was observed in the cells of tested section.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-19N10B7-Immunohistochemistry-Paraffin-NBP2-27396-img0030.jpg)
![Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) [NBP2-27396] - IHC-P detection of HMGB1 protein in a formalin-fixed paraffin-embedded tissue section of human bladder cancer using HMGB1 antibody (clone 19N10B7) at 5 ug/ml concentration. Strong nuclear HMGB1 immunopositivity was observed in the bladder cancer cells whereas the staining was weak in cells of tumor stroma.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-19N10B7-Immunohistochemistry-Paraffin-NBP2-27396-img0031.jpg)
![Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) [NBP2-27396] - IHC-P detection of HMGB1 protein in a formalin-fixed paraffin-embedded tissue section of human stomach cancer using HMGB1 antibody (clone 19N10B7) at 5 ug/ml concentration. This representative image shows a distinct nuclear HMGB1 immunopositivity in the cancerous and sub-mucosal cells.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-19N10B7-Immunohistochemistry-Paraffin-NBP2-27396-img0032.jpg)
![Simple Western: HMGB1/HMG-1 Antibody (19N10B7) [NBP2-27396] - Simple Western lane view shows a specific band for HMGB1 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-19N10B7-Simple-Western-NBP2-27396-img0020.jpg)
![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184] - Analysis of NCAM-1/CD56 antibody (123C3.D5) on Human colon tissue. Incubation: 1 ug/ml for 30 minutes at room temperature. Image from verified customer review.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-123C3-D5-Immunohistochemistry-Paraffin-NBP2-15184-img0019.jpg "Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184] - Analysis of NCAM-1/CD56 antibody (123C3.D5) on Human colon tissue. Incubation: 1 ug/ml for 30 minutes at room temperature. Image from verified customer review.")
![Flow Cytometry: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184] - Flow cytometry of lymphocyte gated PBMCs unstained (gray) orstained with CF568-labeled NCAM-1/CD56 antibody (123C3.D5) (orange).](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-123C3-D5-Flow-Cytometry-NBP2-15184-img0020.jpg)
![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184] - Formalin-fixed, paraffin-embedded human colon Ganglion stained with CD56 Monoclonal Antibody (123C3.D5)](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-123C3-D5-Immunohistochemistry-Paraffin-NBP2-15184-img0016.jpg)
![Flow Cytometry: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184] - Analysis using the Azide and BSA Free version of NBP2-15184. Staining of AF700 conjugated CD56 in human PBMC using anti-CD56 antibody. The primary antibody was used at a dilution of 1:100, incubated for 25 min at 4C in 2% human serum, 0.5 mM EDTA in DPBS. Image from verified customer review.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-123C3-D5-Flow-Cytometry-NBP2-15184-img0014.jpg)
![Simple Western: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184] - Simple Western lane view shows a specific band for NCAM-1/CD56 in 0.2 mg/ml of IMR-32 lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-123C3-D5-Simple-Western-NBP2-15184-img0007.jpg)
![Simple Western: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184] - Electropherogram image of the corresponding Simple Western lane. NCAM-1/CD56 antibody was used at 10 ug/ml dilution of IMR-32 lysates(s) respectively.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-123C3-D5-Simple-Western-NBP2-15184-img0012.jpg)
![Western Blot: Cytokeratin 19 Antibody (4E8) [NBP2-22116] - Western blot analysis using Cytokeratin 19 mouse mAb against T47D (1), MCF-7 (2), HepG2 (3) and SW620 (4) cell lysate.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-4E8-Western-Blot-NBP2-22116-img0003.jpg "Western Blot: Cytokeratin 19 Antibody (4E8) [NBP2-22116] - Western blot analysis using Cytokeratin 19 mouse mAb against T47D (1), MCF-7 (2), HepG2 (3) and SW620 (4) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody (4E8) [NBP2-22116] - Immunofluorescence analysis of HepG2 cells using Cytokeratin 19 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-4E8-Immunocytochemistry-Immunofluorescence-NBP2-22116-img0004.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (4E8) [NBP2-22116] - Immunohistochemical analysis of paraffin-embedded human stomach cancer tissues using Cytokeratin 19 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-4E8-Immunohistochemistry-Paraffin-NBP2-22116-img0007.jpg)
![Western Blot: Cytokeratin 19 Antibody (4E8) [NBP2-22116] - Western blot analysis using Cytokeratin 19 mAb against human Cytokeratin 19 (AA: 115-269) recombinant protein. (Expected MW is 43.1 kDa)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-4E8-Western-Blot-NBP2-22116-img0002.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (4E8) [NBP2-22116] - Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using Cytokeratin 19 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-4E8-Immunohistochemistry-Paraffin-NBP2-22116-img0005.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (4E8) [NBP2-22116] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using Cytokeratin 19 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-4E8-Immunohistochemistry-Paraffin-NBP2-22116-img0006.jpg)
![ELISA: Cytokeratin 19 Antibody (4E8) [NBP2-22116] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-4E8-ELISA-NBP2-22116-img0001.jpg)
![Flow Cytometry: CD11b Antibody (OX-42) [NB100-65284] - Detection of CD11b in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with Mouse Anti-Rat CD11b Monoclonal Antibody (OX-42) [NB100-65284, filled histogram] or Mouse IgG2A Isotype Control Antibody [MAB003, open histogram] followed by anti-Mouse IgG PE-conjugated secondary antibody [F0102B] and Mouse Anti-Rat CD161 Alexa Fluor 647-conjugated Monoclonal Antibody [FAB9766R]. Splenocytes were gated on CD161+ cells.](http://images.novusbio.com/fullsize/CD11b-Antibody-OX-42-Flow-Cytometry-NB100-65284-img0001.jpg "Flow Cytometry: CD11b Antibody (OX-42) [NB100-65284] - Detection of CD11b in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with Mouse Anti-Rat CD11b Monoclonal Antibody (OX-42) [NB100-65284, filled histogram] or Mouse IgG2A Isotype Control Antibody [MAB003, open histogram] followed by anti-Mouse IgG PE-conjugated secondary antibody [F0102B] and Mouse Anti-Rat CD161 Alexa Fluor 647-conjugated Monoclonal Antibody [FAB9766R]. Splenocytes were gated on CD161+ cells.")
![Flow Cytometry: CD11b Antibody (OX-42) [NB100-65284] - Detection of CD11b in Rat Bone Marrow cells by Flow Cytometry. Rat cells were stained with Mouse Anti-Rat CD11b Monoclonal Antibody (OX-42) [NB100-65284, filled histogram] or Mouse IgG2A Isotype Control Antibody [MAB003, open histogram] followed by anti-Mouse IgG APC-conjugated secondary antibody [F0101B] .](http://images.novusbio.com/fullsize/antibody/nb100-65284_mouse-monoclonal-cd11b-antibody-ox-42-flow-cell-surface--31102023115850..jpg)
![Immunocytochemistry/Immunofluorescence: c-Fos Antibody [NBP1-89065] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/c-Fos-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89065-img0011.jpg "Immunocytochemistry/Immunofluorescence: c-Fos Antibody [NBP1-89065] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: c-Fos Antibody [NBP1-89065] - Staining of human stomach shows moderate to strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/c-Fos-Antibody-Immunohistochemistry-Paraffin-NBP1-89065-img0019.jpg)
![Immunohistochemistry-Paraffin: c-Fos Antibody [NBP1-89065] - Staining of human cervix, uterine shows moderate to strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/c-Fos-Antibody-Immunohistochemistry-Paraffin-NBP1-89065-img0016.jpg)
![Immunohistochemistry-Paraffin: c-Fos Antibody [NBP1-89065] - Staining of human kidney shows weak to moderate nuclear positivity in cells in tubules.](http://images.novusbio.com/fullsize/c-Fos-Antibody-Immunohistochemistry-Paraffin-NBP1-89065-img0017.jpg)
![Immunohistochemistry-Paraffin: c-Fos Antibody [NBP1-89065] - Staining of human pancreas shows very weak and few nuclear positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/c-Fos-Antibody-Immunohistochemistry-Paraffin-NBP1-89065-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: SOD2/Mn-SOD Antibody [NBP2-20535] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20535-img0006.jpg "Immunocytochemistry/Immunofluorescence: SOD2/Mn-SOD Antibody [NBP2-20535] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody [NBP2-20535] - Human breast carcinoma. SOD2 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-Immunohistochemistry-Paraffin-NBP2-20535-img0014.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: SOD2/Mn-SOD Antibody [NBP2-20535] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-20535_rabbit-polyclonal-sod2-mn-sod-antibody-1610202419405789.jpg)
![Immunohistochemistry-Paraffin: c-Fos Antibody (2G2) [NBP2-37492] - Analysis of colon cancer tissues using FOS mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/c-Fos-Antibody-2G2-Immunohistochemistry-NBP2-37492-img0005.jpg "Immunohistochemistry-Paraffin: c-Fos Antibody (2G2) [NBP2-37492] - Analysis of colon cancer tissues using FOS mouse mAb with DAB staining.")
![Western Blot: c-Fos Antibody (2G2) [NBP2-37492] - Western blot analysis using FOS mouse mAb against HeLa (1), and HeLa (2) cell lysate.](http://images.novusbio.com/fullsize/c-Fos-Antibody-2G2-Western-Blot-NBP2-37492-img0003.jpg)
![Immunohistochemistry-Paraffin: c-Fos Antibody (2G2) [NBP2-37492] - Analysis of cerebellum tissues using FOS mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/c-Fos-Antibody-2G2-Immunohistochemistry-NBP2-37492-img0006.jpg)
![Flow Cytometry: c-Fos Antibody (2G2) [NBP2-37492] - Flow cytometric analysis of HeLa cells using FOS mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/c-Fos-Antibody-2G2-Flow-Cytometry-NBP2-37492-img0004.jpg)
![Western Blot: c-Fos Antibody (2G2) [NBP2-37492] - Western blot analysis using FOS mAb against human FOS (AA: 116-298) recombinant protein. (Expected MW is 45.8 kDa)](http://images.novusbio.com/fullsize/c-Fos-Antibody-2G2-Western-Blot-NBP2-37492-img0002.jpg)
![ELISA: c-Fos Antibody (2G2) [NBP2-37492] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/c-Fos-Antibody-2G2-ELISA-NBP2-37492-img0001.jpg)
![Western Blot: SERCA2 ATPase Antibody [NB100-237] - Representative Western blot analysis showing SERCA2 bands in HL-1 cells exposed to increasing LDL concentrations (0, 50, 100 and 200 ug/mL) (A) or to similar dose (500 ug/ml) of LDL or HDL (B) for 24 hours. HL-1 cells incubated in absence of LDL. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23516438) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SERCA2-ATPase-Antibody-Western-Blot-NB100-237-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: SERCA2 ATPase Antibody [NB100-237] - Representative Western blot analysis showing SERCA2 bands in HL-1 cells exposed to increasing LDL concentrations (0, 50, 100 and 200 ug/mL) (A) or to similar dose (500 ug/ml) of LDL or HDL (B) for 24 hours. HL-1 cells incubated in absence of LDL. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23516438) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Western Blot: SERCA2 ATPase Antibody [NB100-237] - Detection of human and mouse SERCA2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SERCA2 antibody NB100-237 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/SERCA2-ATPase-Antibody-Western-Blot-NB100-237-img0001.jpg)
![Western Blot: SERCA2 ATPase Antibody [NB100-237] - LDL reduces SERCA expression & SR calcium loading.Representative Western blot analysis showing SERCA2 bands in HL-1 cells exposed to increasing LDL concentrations (0, 50, 100 & 200 µg/mL) (A) or to similar dose (500 µg/ml) of LDL or HDL (B) for 24 hours. The bar graph below shows SERCA2 band quantification. Unchanged levels of beta -tubulin are shown as loading control. Results are mean±SEM of three independent experiments performed in duplicate. *P<0.05 versus. HL-1 cells incubated in absence of LDL. C Calcium images acquired at rest before (CON) & during a rapid caffeine application. D Representative calcium transients recorded in cultures incubated without lipoproteins (CON), with 500 µg/ml LDL or with 500 µg/ml HDL. The lower panel shows the corresponding time integrals of the calcium transients. E summary of the effect of LDL & HDL on the time integral of the caffeine induced calcium transient. *P<0.05 vs. cells incubated with LDL Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0058128), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-237_rabbit-polyclonal-serca2-atpase-antibody-31020241683920.jpg)
![Immunocytochemistry/Immunofluorescence: c-Fos Antibody [NBP2-50057] - Analysis of HeLa cells stained with rabbit pAb to c-FOS, NBP2-50057, dilution 1:5,000 in red, and mouse mAb to tubulin, dilution 1:10,000, in green. The blue is DAPI staining of nuclear DNA. HeLa cells were kept in fetal bovine serum (FBS) free media for 36 hours. Then the cells were treated with PBS (A), as a control, or stimulated with 20% FBS (B) for 30 min. c-FOS antibody labels only the nuclei of stimulated cells. Mouse hippocampus (C) or olfactory bulb sections (D) stained with NBP2-50057, dilution 1:20,000 in red, and mouse mAb to NF-L, dilution 1:5,000, in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The c-FOS antibody stains only nuclei of spontaneously active neurons. NF-L is expressed in axons of neuronal cells.](http://images.novusbio.com/fullsize/c-Fos-Antibody-Western-Blot-NBP2-50057-img0001.jpg "Immunocytochemistry/Immunofluorescence: c-Fos Antibody [NBP2-50057] - Analysis of HeLa cells stained with rabbit pAb to c-FOS, NBP2-50057, dilution 1:5,000 in red, and mouse mAb to tubulin, dilution 1:10,000, in green. The blue is DAPI staining of nuclear DNA. HeLa cells were kept in fetal bovine serum (FBS) free media for 36 hours. Then the cells were treated with PBS (A), as a control, or stimulated with 20% FBS (B) for 30 min. c-FOS antibody labels only the nuclei of stimulated cells. Mouse hippocampus (C) or olfactory bulb sections (D) stained with NBP2-50057, dilution 1:20,000 in red, and mouse mAb to NF-L, dilution 1:5,000, in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The c-FOS antibody stains only nuclei of spontaneously active neurons. NF-L is expressed in axons of neuronal cells.")
![Western Blot: c-Fos Antibody [NBP2-50057] - Analysis of cell lysates using rabbit pAb to c-FOS, NBP2-50057, dilution 1:5,000, in green, and mouse mAb to GAPDH, dilution 1:5,000, in red, used as a loading control. [1] protein standard (red), [2] HeLa cells grown in FBS free media, [3] HeLa cells stimulated with 20% FBS for 2 hours after being in FBS free media for 36 hours, [4] rat cortical neurons, [5] rat cortical neurons treated with membrane depolarization buffer for 5 hours. Multiple bands at 50-65 kDa in stimulated or treated cell lysates, correspond to different isoforms of the c-FOS protein](http://images.novusbio.com/fullsize/c-Fos-Antibody-Western-Blot-NBP2-50057-img0002.jpg)