| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44857 | |||
| Azide and BSA Free | NBP2-34528-1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-96877-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-228SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05423-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62566-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-14024-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-71656SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-78278SS | |||
![Western Blot: GPER/GPR30 Antibody [NBP1-31239] - The MW of GPR30 is estimated to be 42 kDa, but higher MW sizes have been reported due to glycosylation and interaction with other proteins. Bands are identified as glycosylated and nonglycosylated based on reports cited in the text, but could include interaction with other proteins. For each GPER western, the membrane was stripped and reprobed for b-actin as a loading control. Quantitation of GPER was evaluated by summing all immunoreactive bands and dividing by b-actin, then normalizing to HBEC2-KT in each blot. Image collected and cropped by CiteAb from the following publication (biomedcentral.com/1471-2407/12/624), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GPER-GPR30-Antibody-Western-Blot-NBP1-31239-img0012.jpg "Western Blot: GPER/GPR30 Antibody [NBP1-31239] - The MW of GPR30 is estimated to be 42 kDa, but higher MW sizes have been reported due to glycosylation and interaction with other proteins. Bands are identified as glycosylated and nonglycosylated based on reports cited in the text, but could include interaction with other proteins. For each GPER western, the membrane was stripped and reprobed for b-actin as a loading control. Quantitation of GPER was evaluated by summing all immunoreactive bands and dividing by b-actin, then normalizing to HBEC2-KT in each blot. Image collected and cropped by CiteAb from the following publication (biomedcentral.com/1471-2407/12/624), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: GPER/GPR30 Antibody [NBP1-31239] - Detection of GPER/GPR30 in HUVEC nucleus. Image courtesy of a product review by Dr. Subhadeep Chakrabarti of University of Alberta.](http://images.novusbio.com/fullsize/GPER-GPR30-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31239-img0010.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: GPER/GPR30 Antibody [NBP1-31239] - A. 50 ug 293T whole cell lysate/extract. B. 50 ug whole cell lysate/extract of GFP-human GPR30-transfected 293T cells (No boiling). C. 50 ug whole cell lysate/extract of GFP-human GPR30 and GPR30 siRNA-transfected 293T cells (No boiling). 5 % SDS-PAGE GPR30 antibody ) dilution: 1:1000](http://images.novusbio.com/fullsize/GPER-GPR30-Antibody-Western-Blot-NBP1-31239-img0011.jpg)
![Western Blot: GPER/GPR30 Antibody [NBP1-31239] - AhR & GPR30 receptors are present & functional in the MCF10AT1 cells. (A) RT-qPCR analysis of AhR & GPR30 mRNA expression levels represented in arbitrary units (a.u.) in the MCF10AT1 & MCF10CA1a.cl1 cells. MCF-7 cells were used as a control. Values represent mean ± SD of three independent experiments conducted in triplicate. (B) Representative Western blot analyses from three independent experiments of AhR & GPR30 protein expression in MCF10AT1 & MCF10CA1a.cl1 cells. MCF-7 cells were used as a control. (C) XRE-luciferase activity following 8 h exposure of MCF10AT1 cells to ITE at the indicated concentrations. TCDD 10−7 M was used as a control & results were expressed as % of TCDD 10−7 M activity. ***p < 0.001 in Student t-test. (D) XRE-luciferase activity upon 8 h of exposure to ITE 10−10 M alone or in combination with GNF351 at the indicated concentrations. TCDD 10−7 M was used as a control, & results were expressed as % of TCDD 10−7 M activity. Student t-tests revealed the statistically significant differences between unexposed & exposed cells: ***p < 0.001; & between ITE & ITE+GNF351: ###p < 0.001. Values in (C,D) represent mean ± SD of three independent experiments. (E) Representative Western blot analyses from three independent experiments of the phospho-MAPK/MAPK ratio upon exposure of MCF10AT1 cells to G1 (GPR30 agonist) for the times indicated, in the presence or absence of a 2 h pre-treatment with G15 (GPR30 antagonist). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32670863), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-31239_rabbit-polyclonal-gper-gpr30-antibody-310202416171440.jpg)
![Western Blot: GPER/GPR30 Antibody [NBP1-31239] - Effects of short-term exposure of BPA, B[a]P, ITE & G1 10−10 M on AIG & MFE are inhibited by siRNA-AhR & siRNA-GPR30. Representative Western blot analysis from three independent experiments of AhR & GPR30 expression in transfected MCF10AT1 cells with (A) siRNA-AhR, (B) siRNA-GPR30 or their scrambled controls. Quantification of protein expression levels was normalized against tubulin expression. (C,D) Secondary mammospheres formation & (E,F) average number of colonies in soft agar, with the following treatments: BPA and/or B[a]P, G1, or ITE, 10−10 M. Cells were transfected with either siRNA-AhR, siRNA-GPR30 or their scrambled controls before being subjected to the treatments. Treatments were maintained throughout the course of experiments. (mean ± SD of 2 independent experiments, in triplicate). ***p < 0.001, *p < 0.05 vs. their respective unexposed; ###p < 0.001 siRNA vs. scrambled in Student t-test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32670863), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-31239_rabbit-polyclonal-gper-gpr30-antibody-310202416173515.jpg)
![Western Blot: GPER/GPR30 Antibody [NBP1-31239] - Human endothelial cells express GPR30 protein in the cell nucleus.(A) Confluent monolayers of HUVECs from 3 different cords were lysed & the protein lysates were immunoblotted for GPR30. alpha -tubulin was used as loading control. (B) HUVEC monolayers at 30–40% confluence were treated with 40 nM siRNA (control or GPR30_4) for 48 hours prior to lysis followed by immunoblotting of the cell lysates for GPR30. alpha -tubulin was used as loading control. Data shown are mean ± SEM of 4 independent experiments. ** & ## indicate p<0.01 compared to untreated & control siRNA-treated cells, respectively. (C) Confluent HUVECs grown on glass coverslips were fixed, permeabilized & immunostained with anti-GPR30 antibody. Nuclei were stained with Hoechst33342 dye. The merged image shows GPR30 (red) & nuclei (blue) in pseudocolor. Representative images from 3 independent experiments are shown. Bar, 20 µm. (D) Confluent HUVECs were lysed & fractionated into cytosolic (C) & nuclear (N) fractions prior to western blotting for eNOS, GPR30, p65, alpha -tubulin & c-Jun. A representative set of images (obtained from different membranes) from 3 independent experiments is shown. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23285008), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-31239_rabbit-polyclonal-gper-gpr30-antibody-310202416175294.jpg)
![Western Blot: GPER/GPR30 Antibody [NBP1-31239] - Effects of short-term exposure of BPA, B[a]P, ITE & G1 10−10 M on AIG & MFE are inhibited by siRNA-AhR & siRNA-GPR30. Representative Western blot analysis from three independent experiments of AhR & GPR30 expression in transfected MCF10AT1 cells with (A) siRNA-AhR, (B) siRNA-GPR30 or their scrambled controls. Quantification of protein expression levels was normalized against tubulin expression. (C,D) Secondary mammospheres formation & (E,F) average number of colonies in soft agar, with the following treatments: BPA and/or B[a]P, G1, or ITE, 10−10 M. Cells were transfected with either siRNA-AhR, siRNA-GPR30 or their scrambled controls before being subjected to the treatments. Treatments were maintained throughout the course of experiments. (mean ± SD of 2 independent experiments, in triplicate). ***p < 0.001, *p < 0.05 vs. their respective unexposed; ###p < 0.001 siRNA vs. scrambled in Student t-test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32670863), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-31239_rabbit-polyclonal-gper-gpr30-antibody-310202416173516.jpg)
![Western Blot: GPER/GPR30 Antibody [NBP1-31239] - Effects of short-term exposure of BPA, B[a]P, ITE & G1 10−10 M on AIG & MFE are inhibited by siRNA-AhR & siRNA-GPR30. Representative Western blot analysis from three independent experiments of AhR & GPR30 expression in transfected MCF10AT1 cells with (A) siRNA-AhR, (B) siRNA-GPR30 or their scrambled controls. Quantification of protein expression levels was normalized against tubulin expression. (C,D) Secondary mammospheres formation & (E,F) average number of colonies in soft agar, with the following treatments: BPA and/or B[a]P, G1, or ITE, 10−10 M. Cells were transfected with either siRNA-AhR, siRNA-GPR30 or their scrambled controls before being subjected to the treatments. Treatments were maintained throughout the course of experiments. (mean ± SD of 2 independent experiments, in triplicate). ***p < 0.001, *p < 0.05 vs. their respective unexposed; ###p < 0.001 siRNA vs. scrambled in Student t-test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32670863), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-31239_rabbit-polyclonal-gper-gpr30-antibody-310202416163721.jpg)
![Western Blot: Nitrotyrosine Antibody (39B6) [NB110-96877] - Western Blot analysis of Human HEK293 cells showing detection of Nitrotyrosineprotein using Mouse Anti-NitrotyrosineMonoclonal Antibody, Clone 39B6 (NB110-96877). Lane 1: MW Ladder. Lane A: Nitrosylated-HEK293 (15uL). Lane B: HEK293 (15 ug). Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-NitrotyrosineMonoclonal Antibody (NB110-96877) diluted in 1.5% BSA and TBST for 1 hours at RT with shaking . Secondary Antibody: Goat anti-mouse IgG: HRP at 1:4000 for 1 hour at RT with shaking . Predicted/Observed Size: Multiple Bands.](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-39B6-Western-Blot-NB110-96877-img0018.jpg "Western Blot: Nitrotyrosine Antibody (39B6) [NB110-96877] - Western Blot analysis of Human HEK293 cells showing detection of Nitrotyrosineprotein using Mouse Anti-NitrotyrosineMonoclonal Antibody, Clone 39B6 (NB110-96877). Lane 1: MW Ladder. Lane A: Nitrosylated-HEK293 (15uL). Lane B: HEK293 (15 ug). Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-NitrotyrosineMonoclonal Antibody (NB110-96877) diluted in 1.5% BSA and TBST for 1 hours at RT with shaking . Secondary Antibody: Goat anti-mouse IgG: HRP at 1:4000 for 1 hour at RT with shaking . Predicted/Observed Size: Multiple Bands.")
![Immunohistochemistry: Nitrotyrosine Antibody (39B6) [NB110-96877] - Immunohistochemistry analysis using Mouse Anti-NitrotyrosineMonoclonal Antibody, Clone 39B6 (NB110-96877). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-NitrotyrosineMonoclonal Antibody (NB110-96877) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Backskin obtained from transgenic mice.](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-39B6-Immunohistochemistry-NB110-96877-img0021.jpg)
![Western Blot: Nitrotyrosine Antibody (39B6) [NB110-96877] - Western Blot analysis of Human A549 cells showing detection of Multiple Bands Nitrotyrosineprotein using Mouse Anti-Nitrotyrosine Monoclonal Antibody, Clone 39B6 (NB110-96877). Lane 1: MW ladder. Lane 2: Human A549 Cells 15 ug). Load: 15 ug. Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-Nitrotyrosine Monoclonal Antibody (NB110-96877) at 1:1000 for 2.5 hours at RT with shaking . Secondary Antibody: Goat anti-mouse IgG:HRP at 1:1000 for 1 hour at RT with shaking . Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: Multiple Bands.](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-39B6-Western-Blot-NB110-96877-img0016.jpg)
![Western Blot: Nitrotyrosine Antibody (39B6) [NB110-96877] - Western Blot analysis of Human Recombinant Protein showing detection of Multiple Bands Nitrotyrosineprotein using Mouse Anti-NitrotyrosineMonoclonal Antibody, Clone 39B6 (NB110-96877). Lane 1: MW Ladder. Lane 2: hASYN Monomer (3.84 ug). Lane 3: Nitrosylated hASYN (3.84 ug).. Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-NitrotyrosineMonoclonal Antibody (NB110-96877) at 1:1000 for 2 hours at RT with shaking . Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 2 hour at RT with shaking . Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: Multiple Bands.](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-39B6-Western-Blot-NB110-96877-img0017.jpg)
![Immunohistochemistry: Nitrotyrosine Antibody (39B6) [NB110-96877] - Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Nitrotyrosine Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-39B6-Immunohistochemistry-NB110-96877-img0014.jpg)
![Immunohistochemistry-Paraffin: Nitrotyrosine Antibody (39B6) [NB110-96877] - Immuno-peroxidase (DAB) staining for nitrotyrosine (upper row) and immuno-alkaline phosphatase (ALP) staining for 8-OHdG (bottom row), hematoxylin counterstain. Representative small intestinal tumor histopathology of Category 1 (low-grade adenoma) in ApcMin/+ (left column) and ApcMin/+ Mieap-/- (second from left column) mice, Category 2 (high-grade adenoma, second from right column) and Category 3 (adenocarcinoma, right column) in the ApcMin/+ Mieap-/- mice. Scale bars: 100 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep12472), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-39B6-Immunohistochemistry-Paraffin-NB110-96877-img0015.jpg)
![Immunohistochemistry: Nitrotyrosine Antibody (39B6) [NB110-96877] - Immunohistochemistry analysis using Mouse Anti-NitrotyrosineMonoclonal Antibody, Clone 39B6 (NB110-96877). Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-NitrotyrosineMonoclonal Antibody (NB110-96877) at 1:1000000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Magnification: 40x. With anti-microbial. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications.](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-39B6-Immunohistochemistry-NB110-96877-img0019.jpg)
![Immunohistochemistry: Nitrotyrosine Antibody (39B6) [NB110-96877] - Immunohistochemistry analysis using Mouse Anti-NitrotyrosineMonoclonal Antibody, Clone 39B6 (NB110-96877). Tissue: liver tissue . Species: Rat. Primary Antibody: Mouse Anti-NitrotyrosineMonoclonal Antibody (NB110-96877) at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green).](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-39B6-Immunohistochemistry-NB110-96877-img0020.jpg)
![Immunohistochemistry: Nitrotyrosine Antibody (39B6) [NB110-96877] - Immunohistochemistry analysis using Mouse Anti-NitrotyrosineMonoclonal Antibody, Clone 39B6 (NB110-96877). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-NitrotyrosineMonoclonal Antibody (NB110-96877) at 1:25000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Magnification: 40x.](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-39B6-Immunohistochemistry-NB110-96877-img0022.jpg)
![Immunocytochemistry/Immunofluorescence: SCP1 Antibody [NB300-228] - SCP1 labeled in mouse pachytene preparation (red), using NB 300-228. CDK2 staining, near teleomeres, is also present (green).](http://images.novusbio.com/fullsize/SCP1-Antibody-Immunocytochemistry-Immunofluorescence-NB300-228-img0003.jpg "Immunocytochemistry/Immunofluorescence: SCP1 Antibody [NB300-228] - SCP1 labeled in mouse pachytene preparation (red), using NB 300-228. CDK2 staining, near teleomeres, is also present (green).")
![Simple Western: SCP1 Antibody [NB300-228] - Lane view shows a specific band for SCP1 in 0.5 mg/ml of Human Testis (left) and Mouse Testis (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody](http://images.novusbio.com/fullsize/SCP1-Antibody-Simple-Western-NB300-228-img0006.jpg)
![Western Blot: Peripherin Antibody [NBP1-05423] - Analysis of spinal cord tissue lysates (lanes 2-5) and cell lysates (lanes 6 and 7) using chicken pAb to peripherin, NBP1-05423, dilution 1:10,000 in green: [1] protein standard (red), [2] rat, [3] mouse, [4] pig, [5] cow spinal cord; [6] SH-SY5Y, and [7] PC12 cells. The band at 57kDa corresponds to peripherin protein.](http://images.novusbio.com/fullsize/Peripherin-Antibody-Western-Blot-NBP1-05423-img0003.jpg "Western Blot: Peripherin Antibody [NBP1-05423] - Analysis of spinal cord tissue lysates (lanes 2-5) and cell lysates (lanes 6 and 7) using chicken pAb to peripherin, NBP1-05423, dilution 1:10,000 in green: [1] protein standard (red), [2] rat, [3] mouse, [4] pig, [5] cow spinal cord; [6] SH-SY5Y, and [7] PC12 cells. The band at 57kDa corresponds to peripherin protein.")
![Immunocytochemistry/Immunofluorescence: Peripherin Antibody [NBP1-05423] - Analysis of mixed fibroblast and PC12 pheochromocytoma cell culture stained with chicken pAb to peripherin, NBP1-05423, dilution 1:5,000 in green, and costained with rabbit pAb to vimentin, RPCA-Vim, dilution 1:2,000, in red. The blue is DAPI staining of nuclear DNA. NBP1-05423 antibody reveals cytoplasmic filamentous staining in the small PC12 cells, while vimentin antibody stains intermediate filaments in the surrounding fibroblastic cells which do not express peripherin.](http://images.novusbio.com/fullsize/Peripherin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-05423-img0005.jpg)
![Immunohistochemistry-Frozen: Peripherin Antibody [NBP1-05423] - Peripherin staining (red) in nerve bundles in the human larynx. Image from verified customer review.](http://images.novusbio.com/fullsize/Peripherin-Antibody-Immunohistochemistry-Frozen-NBP1-05423-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Peripherin Antibody [NBP1-05423] - shows rat mixed neuron/glial cultures stained with NBP1-05423, our chicken polyclonal antibody to peripherin (green channel) and rabbit antibody to the neurofilament subunit alpha-internexin NBP1-05207 (green channel). These cultures contain mostly neurons which are rich in alpha-internexin, and a subgroup which have a large amount of peripherin also, such as the prominent cell in the middle of the micrograph. Since this cell expresses large amounts of peripherin and alpha-internexin, the green and red signals superimpose to produce a golden cell. Blue is a DNA stain.](http://images.novusbio.com/fullsize/Peripherin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-05423-img0001.jpg)
![Immunohistochemistry-Frozen: Peripherin Antibody [NBP1-05423] - Peripherin staining (green) in the mouse brainstem including the spinal trigeminal tract (right side of image) and arching fiber bundle to the solitary nuclear tract (center). Nissl Counterstain. Image from verified customer review.](http://images.novusbio.com/fullsize/Peripherin-Antibody-Immunohistochemistry-Frozen-NBP1-05423-img0006.jpg)
![Western Blot: USP9x Antibody [NBP1-48321] - WT1 is a substrate of pUSP9X (serine 2563) in mitosis. Co-immunoprecipitation of FLAG-tagged WT1 with endogenous USP9X from HEK 293T cells (EV, expression vector). Cells were exposed to nocodazole, collected, lysed (WCE, whole cell extracts), and subjected to anti-FLAG immunoprecipitation before analysis by western blot. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-020-15059-5), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/USP9x-Antibody-Western-Blot-NBP1-48321-img0010.jpg "Western Blot: USP9x Antibody [NBP1-48321] - WT1 is a substrate of pUSP9X (serine 2563) in mitosis. Co-immunoprecipitation of FLAG-tagged WT1 with endogenous USP9X from HEK 293T cells (EV, expression vector). Cells were exposed to nocodazole, collected, lysed (WCE, whole cell extracts), and subjected to anti-FLAG immunoprecipitation before analysis by western blot. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-020-15059-5), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: USP9x Antibody [NBP1-48321] - Immunocytochemical analysis of USP9X in NTERA-2 cells](http://images.novusbio.com/fullsize/USP9x-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-48321-img0005.jpg)
![Immunohistochemistry: USP9x Antibody [NBP1-48321] - IHC staining of Usp9x in brain tissue.](http://images.novusbio.com/fullsize/USP9x-Antibody-Immunohistochemistry-NBP1-48321-img0006.jpg)
![Western Blot: USP9x Antibody [NBP1-48321] - Analysis of USP9X in Caco-2 whole cell lysates.](http://images.novusbio.com/fullsize/USP9x-Antibody-Western-Blot-NBP1-48321-img0007.jpg)
![Western Blot: USP9x Antibody [NBP1-48321] - Analysis of USP9X in NIH/3T3 whole cell lysate.](http://images.novusbio.com/fullsize/USP9x-Antibody-Western-Blot-NBP1-48321-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: USP9x Antibody [NBP1-48321] - ICC/IC analysis of USP9x in mouse ES cell line 129. Image courtesy of anonymous customer product review.](http://images.novusbio.com/fullsize/USP9x-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-48321-img0009.jpg)
![Western Blot: APP Antibody (6E10) - Chimeric [NBP2-62566] - Anti-Amyloid beta antibody 6E10. Human brain cerebral cortex (A), frontal cortex (B) and cerebellum (C) lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of 6E10 (NBP2-62566) at 0.3 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Amyloid beta is ~35kDa, though this protein is known to form SDS-resistant low-molecular weight oligomers as seen in this blot (i.e. dimers, c.f. Kumar et al. PMID: 21527912). NBP2-62566 successfully detected aggregate human Amyloid beta in human brain cerebral cortex, frontal cortex and cerebellum lysate samples.](http://images.novusbio.com/fullsize/APP-Antibody-6E10-Chimeric-Western-Blot-NBP2-62566-img0002.jpg "Western Blot: APP Antibody (6E10) - Chimeric [NBP2-62566] - Anti-Amyloid beta antibody 6E10. Human brain cerebral cortex (A), frontal cortex (B) and cerebellum (C) lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of 6E10 (NBP2-62566) at 0.3 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Amyloid beta is ~35kDa, though this protein is known to form SDS-resistant low-molecular weight oligomers as seen in this blot (i.e. dimers, c.f. Kumar et al. PMID: 21527912). NBP2-62566 successfully detected aggregate human Amyloid beta in human brain cerebral cortex, frontal cortex and cerebellum lysate samples.")
![Immunohistochemistry-Paraffin: APP Antibody (6E10) - Chimeric [NBP2-62566] - Staining of human Alzheimer's disease hippocampus tissue using anti-Amyloid Beta antibody. 6E10 Anti-Amyloid Beta staining of paraffin embedded human hippocampus affected by Alzheimer's disease using the rabbit-chimeric version of 6E10 (NBP2-62566). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP2-62566 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Staining of amyloid plaques in the parahippocampal gyrus may be observed. Recommended concentration, 2-4 ug/ml.](http://images.novusbio.com/fullsize/APP-Antibody-6E10-Chimeric-Immunohistochemistry-Paraffin-NBP2-62566-img0001.jpg)
![Immunohistochemistry-Frozen: APP Antibody (6E10) - Chimeric [NBP2-62566] - 6E10 staining reveals amyloid plaque in frontal cortex of AD mouse model. Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-62566_rabbit-monoclonal-app-antibody-6e10-chimeric-immunohistochemistry-frozen-284202314243..jpg)
![Immunohistochemistry-Frozen: APP Antibody (6E10) - Chimeric [NBP2-62566] - Plaque staining in AD Mouse model (Hippocampus) - without formic acid pretreatment. Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-62566_rabbit-monoclonal-app-antibody-6e10-chimeric-immunohistochemistry-frozen-672023103423..jpg)
![Immunohistochemistry-Paraffin: Irisin/FNDC5 Antibody [NBP2-14024] - Specific positive staining can be observed in a subpopulation of mouse gastric mucosa cells. Green is FNDC5. Image from verified customer review.](http://images.novusbio.com/fullsize/Irisin-FNDC5-Antibody-Immunohistochemistry-Paraffin-NBP2-14024-img0011.jpg "Immunohistochemistry-Paraffin: Irisin/FNDC5 Antibody [NBP2-14024] - Specific positive staining can be observed in a subpopulation of mouse gastric mucosa cells. Green is FNDC5. Image from verified customer review.")
![Immunohistochemistry-Paraffin: Irisin/FNDC5 Antibody [NBP2-14024] - Staining of human skeletal muscle shows granular cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/Irisin-FNDC5-Antibody-Immunohistochemistry-Paraffin-NBP2-14024-img0007.jpg)
![Western Blot: Irisin/FNDC5 Antibody [NBP2-14024] - Comparison of Irisin/FNDC5 expression levels detected by Western blot in Normal Human Keratinocytes (HaCat) and Larynx Epidermoid Carcinoma 2 (HEp-2) (A). The mean value of the densitometric optical analysis of the 3 repeats of Western blot detecting irisin expression levels (B). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35053200/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp2-14024_rabbit-polyclonal-irisin-fndc5-antibody-2552023151496.jpg)
![Immunohistochemistry: Irisin/FNDC5 Antibody [NBP2-14024] - Comparison of irisin expression using immunohistochemistry (IHC; positive reactions—brown cell cytoplasm) in control tissue (A) & at different grades of malignancy (G) of breast cancer (BC; (B)—G1; (C)—G2, in apical parts of cells; (D)—G3) with Ki-67 (E) & PGC-1 alpha (F); magnification x200. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35408891), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-14024_rabbit-polyclonal-irisin-fndc5-antibody-310202415291921.jpg)
![Immunohistochemistry: Irisin/FNDC5 Antibody [NBP2-14024] - Positive immunohistochemical (IHC) reactions (brown color) indicating strong irisin expression in the apical parts of cancer cells & vesicles; magnification x400. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35408891), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-14024_rabbit-polyclonal-irisin-fndc5-antibody-310202415363743.jpg)
![Immunohistochemistry: Irisin/FNDC5 Antibody [NBP2-14024] - Comparison of irisin expression using immunohistochemistry (IHC; positive reactions—brown cell cytoplasm) in control tissue (A) & at different grades of malignancy (G) of breast cancer (BC; (B)—G1; (C)—G2, in apical parts of cells; (D)—G3) with Ki-67 (E) & PGC-1 alpha (F); magnification x200. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35408891), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-14024_rabbit-polyclonal-irisin-fndc5-antibody-310202415384153.jpg)
![Immunohistochemistry: Irisin/FNDC5 Antibody [NBP2-14024] - Comparison of irisin expression using immunohistochemistry (IHC; positive reactions—brown cell cytoplasm) in control tissue (A) & at different grades of malignancy (G) of breast cancer (BC; (B)—G1; (C)—G2, in apical parts of cells; (D)—G3) with Ki-67 (E) & PGC-1 alpha (F); magnification x200. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35408891), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-14024_rabbit-polyclonal-irisin-fndc5-antibody-310202415392544.jpg)
![Immunohistochemistry: Irisin/FNDC5 Antibody [NBP2-14024] - Comparison of irisin expression using immunohistochemistry (IHC; positive reactions—brown cell cytoplasm) in control tissue (A) & at different grades of malignancy (G) of breast cancer (BC; (B)—G1; (C)—G2, in apical parts of cells; (D)—G3) with Ki-67 (E) & PGC-1 alpha (F); magnification x200. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35408891), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-14024_rabbit-polyclonal-irisin-fndc5-antibody-310202415384123.jpg)
![Immunohistochemistry: Irisin/FNDC5 Antibody [NBP2-14024] - Comparison of irisin expression using immunohistochemistry (IHC; positive reactions—brown cell cytoplasm) in control tissue (A) & at different grades of malignancy (G) of breast cancer (BC; (B)—G1; (C)—G2, in apical parts of cells; (D)—G3) with Ki-67 (E) & PGC-1 alpha (F); magnification x200. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35408891), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-14024_rabbit-polyclonal-irisin-fndc5-antibody-310202415384172.jpg)
![Western Blot: Irisin/FNDC5 Antibody [NBP2-14024] - Comparison of irisin expression levels detected by Western blot in Normal Human Keratinocytes (HaCat) & Larynx Epidermoid Carcinoma 2 (HEp-2) (A). The mean value of the densitometric optical analysis of the 3 repeats of Western blot detecting irisin expression levels (B). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35053200), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-14024_rabbit-polyclonal-irisin-fndc5-antibody-310202415171334.jpg)
![Immunohistochemistry: Irisin/FNDC5 Antibody [NBP2-14024] - Comparison of irisin expression using immunohistochemistry (IHC; positive reactions—brown cell cytoplasm) in control tissue (A) & at different grades of malignancy (G) of breast cancer (BC; (B)—G1; (C)—G2, in apical parts of cells; (D)—G3) with Ki-67 (E) & PGC-1 alpha (F); magnification x200. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35408891), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-14024_rabbit-polyclonal-irisin-fndc5-antibody-31020241530429.jpg)
![Western Blot: FUS Antibody [NB100-561] - Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbitanti-FUS antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with anexposure time of 3 seconds.](http://images.novusbio.com/fullsize/FUS-Antibody-Western-Blot-NB100-561-img0023.jpg "Western Blot: FUS Antibody [NB100-561] - Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbitanti-FUS antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with anexposure time of 3 seconds.")
![Immunohistochemistry-Paraffin: FUS Antibody [NB100-561] - Detection of mouse FUS by immunohistochemistry. Sample: FFPE section of mouse teratoma Antibody: Affinity purified rabbit anti-FUS (NB100-561) used at 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/FUS-Antibody-Immunohistochemistry-Paraffin-NB100-561-img0024.jpg)
![Immunohistochemistry-Paraffin: FUS Antibody [NB100-561] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-FUS (NB100-561). Detection: DAB](http://images.novusbio.com/fullsize/FUS-Antibody-Immunohistochemistry-NB100-561-img0020.jpg)
![Immunoprecipitation: FUS Antibody [NB100-561] - FUS immunoprecipitation in human fibroblast cell lysates. Image from verified customer review.](http://images.novusbio.com/fullsize/FUS-Antibody-Immunoprecipitation-NB100-561-img0005.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: FUS Antibody [NB100-561] - Detection of human FUS by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FUS antibody NB100-561 used for IP at 3 ug per reaction. FUS was also immunoprecipitated by rabbit anti-FUS recombinant monoclonal antibody [BLR023E] (NBP2-76415) and rabbit anti-FUS antibody NB100-565. For blotting immunoprecipitated FUS, NBP2-76415 was used at 1:1000. Detection: Chemiluminescence with an exposure time time of 3 minutes.](http://images.novusbio.com/fullsize/FUS-Antibody-Immunoprecipitation-NB100-561-img0021.jpg)
![Western Blot: OPA1 Antibody (1E8-1D9) [NBP1-71656] - Analysis of OPA1 expression in 1) HeLa 2) MEF 3) HepG2 4) A431 5) CHO 6)PC12 and 7) Ntera2 whole cell lysates using NBP1-71656.](http://images.novusbio.com/fullsize/OPA1-Antibody-1E8-1D9-Western-Blot-NBP1-71656-img0009.jpg "Western Blot: OPA1 Antibody (1E8-1D9) [NBP1-71656] - Analysis of OPA1 expression in 1) HeLa 2) MEF 3) HepG2 4) A431 5) CHO 6)PC12 and 7) Ntera2 whole cell lysates using NBP1-71656.")
![Immunocytochemistry/Immunofluorescence: OPA1 Antibody (1E8-1D9) [NBP1-71656] - OPA1 antibody was tested in ARPE-19 cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/OPA1-Antibody-1E8-1D9-Immunocytochemistry-Immunofluorescence-NBP1-71656-img0007.jpg)
![Immunohistochemistry: OPA1 Antibody (1E8-1D9) [NBP1-71656] - Analysis of OPA1 on mouse skin using NBP1-71656.](http://images.novusbio.com/fullsize/OPA1-Antibody-1E8-1D9-Immunohistochemistry-NBP1-71656-img0008.jpg)
![Simple Western: OPA1 Antibody (1E8-1D9) [NBP1-71656] - Image shows a specific band for OPA1 in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/OPA1-Antibody-1E8-1D9-Simple-Western-NBP1-71656-img0010.jpg)
![Simple Western: OPA1 Antibody (1E8-1D9) - BSA Free [NBP1-71656] - Image shows a specific band for OPA1 in 0.1 ug/uL mouse hippocampus tissue lysate. Primary antibody dilution: 1:200. Image from verified customer review.](http://images.novusbio.com/fullsize/OPA1-Antibody-1E8-1D9-BSA-Free-Simple-Western-NBP1-71656-img0011.jpg)
![Western Blot: Cytokeratin 19 Antibody [NBP1-78278] - Analysis of Cytokeratin 19 in MCF7 lysate.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-Western-Blot-NBP1-78278-img0006.jpg "Western Blot: Cytokeratin 19 Antibody [NBP1-78278] - Analysis of Cytokeratin 19 in MCF7 lysate.")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody [NBP1-78278] - Antibody at 1:25 in MCF7 cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78278-img0004.jpg)
![Immunohistochemistry: Cytokeratin 19 Antibody [NBP1-78278] - Analysis of Cytokeratin 19 in human kidney carcinoma using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-Immunohistochemistry-NBP1-78278-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody [NBP1-78278] - Confocal immunofluorescent analysis of HepG2 cells using Cytokeratin 19 antibody (NBP1-78278, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78278-img0007.jpg)
![Simple Western: Cytokeratin 19 Antibody [NBP1-78278] - Image shows a specific band for Cytokeratin 19 in 0.05 mg/mL of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-Simple-Western-NBP1-78278-img0008.jpg)
![Western Blot: NF-L Antibody [NB300-131] - Analysis of different tissue lysates using rabbit pAb to NF-L, NB300-131, dilution 1:20,000. in green. [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. The strong band at 68kDa corresponds to the NF-L protein.](http://images.novusbio.com/fullsize/NF-L-Antibody-Western-Blot-NB300-131-img0004.jpg "Western Blot: NF-L Antibody [NB300-131] - Analysis of different tissue lysates using rabbit pAb to NF-L, NB300-131, dilution 1:20,000. in green. [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. The strong band at 68kDa corresponds to the NF-L protein.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: NF-L Antibody [NB300-131] - Neurofilament (NF-L antibody) used as axon marker in WT and KO mice using immunofluorescence staining to illustrate the axon morphology of retinal ganglion cells. Central part of retina is enlarged and displayed in WT and KO mice. Scale bars are 500 micrometers for left and 200 micrometers for right. Citation: Lin Y-S, Kuo K-T, Chen S-K, Huang H-S (2018) RBFOX3/NeuN is dispensable for visual function. PLoS ONE 13(2): e0192355. //doi.org/10.1371/journal.pone.0192355](http://images.novusbio.com/fullsize/NF-L-Antibody-Immunofluorescence-NB300-131-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: 68kDa Neurofilament Light Antibody [NB300-131] - Mixed neuron/glia cultures from newborn rat brain stained with NB300-220 antibody to peripherin (green) and rabbit polyclonal antibody to NF-L RPCA-NF-L (red channel). A class of large neurons, like the one in the middle of this image, contain peripherin, while the majority of neurons and their processes contain NF-L and not peripherin. Interestingly, the periperin positive cells often contain a cytoplasmic inclusion next to the nucleus which stains for both peripherin and NF-L, and so appears golden in this kind of image. The blue channel reveals the localization of DNA](http://images.novusbio.com/fullsize/68kDa-Neurofilament-Light-Antibody-Immunocytochemistry-Immunofluorescence-NB300-131-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: NF-L Antibody [NB300-131] - Analysis of mouse cerebellum section stained with rabbit pAb to NF-L, NB300-131, dilution 1:5,000 in red, and costained with chicken pAb to MBP, dilution 1:5,000, in green. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. NB300-131 antibody labels dendrites and axons of neuronal cells, and MBP antibody stains the network of myelin sheathes around axons.](http://images.novusbio.com/fullsize/NF-L-Antibody-Immunocytochemistry-Immunofluorescence-NB300-131-img0003.jpg)