| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-36570SS | |||
| Azide and BSA Free | NBP2-80608 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-13169SS | |||
| Azide and BSA Free | NBP2-80553 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-93504-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44055 | |||
| BSA Free | NBP2-52874-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61745-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-47365-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85519-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89112-25ul | |||
![Western Blot: Calnexin Antibody (1C2.2D11) [NBP2-36570] - Analysis of 11 kDa Partial Recombinant Human Calnexin protein with Calnexin antibody (clone 1C2.2D11) at 0.5 ug/mL.](http://images.novusbio.com/fullsize/Calnexin-Antibody-1C2-2D11-Western-Blot-NBP2-36570-img0008.jpg "Western Blot: Calnexin Antibody (1C2.2D11) [NBP2-36570] - Analysis of 11 kDa Partial Recombinant Human Calnexin protein with Calnexin antibody (clone 1C2.2D11) at 0.5 ug/mL.")
![Immunocytochemistry/Immunofluorescence: Calnexin Antibody (1C2.2D11) [NBP2-36570] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-Calnexin (1C2.2D11) conjugated to FITC [NBP2-36570F] at 10ug/mL for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Calnexin-Antibody-1C2-2D11-Immunocytochemistry-Immunofluorescence-NBP2-36570-img0012.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody (1C2.2D11) [NBP2-36570] - Analysis of FFPE tissue section of normal human kidney using mouse monoclonal Calnexin antibody (clone 1C2.2D11) at 7 ug/mL concentration. The cells of Glomeruli developed strong cytoplasmic staining.](http://images.novusbio.com/fullsize/Calnexin-Antibody-1C2-2D11-Immunohistochemistry-Paraffin-NBP2-36570-img0010.jpg)
![Flow (Intracellular): Calnexin Antibody (1C2.2D11) [NBP2-36570] - An intracellular stain was performed on HeLa cells with Calnexin [1C2.2D11] Antibody NBP2-36570AF594 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 594.](http://images.novusbio.com/fullsize/Calnexin-Antibody-1C2-2D11-Flow-Intracellular-NBP2-36570-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: Calnexin Antibody (1C2.2D11) [NBP2-36570] - Calnexin (1C2.2D11) antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Calnexin-Antibody-1C2-2D11-Immunocytochemistry-Immunofluorescence-NBP2-36570-img0009.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody (1C2.2D11) [NBP2-36570] - Analysis of FFPE tissue section of normal human breast using mouse monoclonal Calnexin antibody (clone 1C2.2D11) at 7 ug/mL concentration. The myoepithelial cells around the lobules depicted a very strong cytoplasmic staining.](http://images.novusbio.com/fullsize/Calnexin-Antibody-1C2-2D11-Immunohistochemistry-Paraffin-NBP2-36570-img0003.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody (1C2.2D11) [NBP2-36570] - Analysis of FFPE tissue section of malignant stromal tumor of the human small bowel using mouse monoclonal Calnexin antibody (clone 1C2.2D11) at 7 ug/mL concentration. The cancer cells showed a very strong cytoplasmic reactivity for Calnexin.](http://images.novusbio.com/fullsize/Calnexin-Antibody-1C2-2D11-Immunohistochemistry-Paraffin-NBP2-36570-img0005.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody (1C2.2D11) [NBP2-36570] - Analysis of FFPE tissue section of human placenta using mouse monoclonal Calnexin antibody (clone 1C2.2D11) at 7 ug/mL concentration.](http://images.novusbio.com/fullsize/Calnexin-Antibody-1C2-2D11-Immunohistochemistry-Paraffin-NBP2-36570-img0006.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody (1C2.2D11) [NBP2-36570] - Analysis of FFPE tissue section of human skin using mouse monoclonal Calnexin antibody (clone 1C2.2D11) at 7 ug/mL concentration. The outermost keratinocytes layer of the epidermis showed cytoplasmic positivity for Calnexin protein.](http://images.novusbio.com/fullsize/Calnexin-Antibody-1C2-2D11-Immunohistochemistry-Paraffin-NBP2-36570-img0007.jpg)
![Flow (Intracellular): Calnexin Antibody (1C2.2D11) [NBP2-36570] - Intracellular staining of human Calnexin in Flow cytometry using 5.0 ug of antibody per 1 million cells. Isotype control was mouse IgG2b kappa.](http://images.novusbio.com/fullsize/Calnexin-Antibody-1C2-2D11-Flow-Intracellular-NBP2-36570-img0002.jpg)
![Flow (Intracellular): Calnexin Antibody (1C2.2D11) [NBP2-36570] - An intracellular stain was performed on HeLa cells with Calnexin Antibody (1C2.2D11) NBP2-36570F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.](http://images.novusbio.com/fullsize/Calnexin-Antibody-1C2-2D11-Flow-Intracellular-NBP2-36570-img0011.jpg)
![Western Blot: A2BP1 Antibody (D8H8) [NBP2-13169] - Western blot analysis of A2BP1 expression in 1) human brain and 2) mouse brain tissue lysates.](http://images.novusbio.com/fullsize/A2BP1-Antibody-D8H8-Western-Blot-NBP2-13169-img0003.jpg "Western Blot: A2BP1 Antibody (D8H8) [NBP2-13169] - Western blot analysis of A2BP1 expression in 1) human brain and 2) mouse brain tissue lysates.")
![Immunocytochemistry/Immunofluorescence: A2BP1 Antibody (D8H8) [NBP2-13169] - A2BP1 antibody was tested in Neuro-2a cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/A2BP1-Antibody-D8H8-Immunocytochemistry-Immunofluorescence-NBP2-13169-img0004.jpg)
![Immunohistochemistry: A2BP1 Antibody (D8H8) [NBP2-13169] - IHC staining of A2BP1 in mouse brain using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/A2BP1-Antibody-D8H8-Immunohistochemistry-NBP2-13169-img0005.jpg)
![Simple Western: A2BP1 Antibody (D8H8) [NBP2-13169] - Simple Western lane view shows a specific band for A2BP1 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/A2BP1-Antibody-D8H8-Simple-Western-NBP2-13169-img0006.jpg)
![Western Blot: Calnexin Antibody (1563) [NBP2-43765] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 7.5 % SDS-PAGE Calnexin antibody [1563] dilution: 1:1000.](http://images.novusbio.com/fullsize/Calnexin-Antibody-1563-Western-Blot-NBP2-43765-img0001.jpg "Western Blot: Calnexin Antibody (1563) [NBP2-43765] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 7.5 % SDS-PAGE Calnexin antibody [1563] dilution: 1:1000.")
![Western Blot: Calnexin Antibody (1563) [NBP2-43765] - Analysis of A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat-2 whole cell lysate/extract 7.5 % SDS-PAGE Calnexin antibody [1563] dilution: 1:500](http://images.novusbio.com/fullsize/Calnexin-Antibody-1563-Western-Blot-NBP2-43765-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: Calnexin Antibody (1563) [NBP2-43765] - Calnexin antibody [GT1563] detects Calnexin protein at cytoplasm by immunofluorescent analysis.Sample: Neuro2A cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Calnexin stained by Calnexin antibody [GT1563] (NBP2-43765) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-43765_mouse-monoclonal-calnexin-antibody-1563-91020242001038.jpg)
![Western Blot: Calnexin Antibody (1563) [NBP2-43765] - Analysis of A. 50 ug mouse liver lysate/extract 7.5 % SDS-PAGE Calnexin antibody [1563] dilution: 1:1000](http://images.novusbio.com/fullsize/Calnexin-Antibody-1563-Western-Blot-NBP2-43765-img0003.jpg)
![Western Blot: Calnexin Antibody (1563) [NBP2-43765] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [GT1563] (NBP2-43765) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43765_mouse-monoclonal-calnexin-antibody-1563-91020242075941.jpg)
![Western Blot: Calnexin Antibody (1563) [NBP2-43765] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [GT1563] (NBP2-43765) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43765_mouse-monoclonal-calnexin-antibody-1563-910202420134012.jpg)
![<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) 293T whole cell extracts (15 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [GT1563] (NBP2-43765) diluted at 1:6000.](http://images.novusbio.com/fullsize/nbp2-43765_mouse-monoclonal-calnexin-antibody-1563-western-blot-261120251833102.jpg)
![Calnexin antibody [GT1563] detects Calnexin protein by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Calnexin stained by Calnexin antibody [GT1563] (NBP2-43765) diluted at 1:500.Red: Calnexin, stained by Calnexin antibody [C3], C-term diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-43765_mouse-monoclonal-calnexin-antibody-1563-immunocytochemistry-immunofluorescence-261120251847323.jpg)
![Immunohistochemistry-Paraffin: C9orf72 Antibody [NBP1-93504] - Staining of human cerebral cortex shows weak to moderate cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/C9orf72-Antibody-Immunohistochemistry-Paraffin-NBP1-93504-img0004.jpg "Immunohistochemistry-Paraffin: C9orf72 Antibody [NBP1-93504] - Staining of human cerebral cortex shows weak to moderate cytoplasmic positivity in neurons.")
![Immunohistochemistry-Paraffin: C9orf72 Antibody [NBP1-93504] - Staining of human gastrointestinal shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/C9orf72-Antibody-Immunohistochemistry-Paraffin-NBP1-93504-img0002.jpg)
![Western Blot: FUS Antibody [NB100-562] - Western blot shows lysates of HEK293 human embryonic kidney parental cell line and FUS knockout (KO) HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human FUS Polyclonal Antibody (Catalog # NB100-562) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for FUS at approximately 75 kDa (as indicated) in the parental HEK293 celll line, but is not detectable in the knockout HEK293 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/FUS-Antibody-Knockout-Validated-NB100-562-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: FUS Antibody [NB100-562] - Western blot shows lysates of HEK293 human embryonic kidney parental cell line and FUS knockout (KO) HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human FUS Polyclonal Antibody (Catalog # NB100-562) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for FUS at approximately 75 kDa (as indicated) in the parental HEK293 celll line, but is not detectable in the knockout HEK293 cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: FUS Antibody [NB100-562] - Detection of human FUS by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-FUS antibody NB100-562 (lot NB100-562-2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. FUS was also immunoprecipitated by a previous lot (lot NB100-562-1) of this antibody. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/FUS-Antibody-Western-Blot-NB100-562-img0010.jpg)
![Immunohistochemistry-Paraffin: FUS Antibody [NB100-562] - Section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-FUS used at a dilution of 1:5,000 (0.2ug/ml) and 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/FUS-Antibody-Immunohistochemistry-Paraffin-NB100-562-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: FUS Antibody [NB100-562] - Detection of human FUS on HeLa whole cell lysate using NB100-562. For IP in B, rabbit anti-FUS antibodies NB100-560, NB100-561, NB100-562 and NB100-565 were used.](http://images.novusbio.com/fullsize/FUS-Antibody-Western-Blot-NB100-562-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: FUS Antibody (CL0190) [NBP2-52874] - Immunofluorescence of HeLa wildtype (WT) and FUS knockout (KO) cells labelled with a green or a far-red fluorescent dye, respectively. WT and KO cells were mixed and plated to a 1:1 ratio on coverslips. Cells were stained with Mouse Anti-FUS Monoclonal Antibody (Catalog #NBP2-52874) at 1:1000, followed by the corresponding Alexa Fluor 555-coupled Secondary Antibody. Acquisition of the green (identification of WT cells), red (antibody staining) and far-red (identification of KO cells) channels was performed. Representative image of the red (grayscale) channel is shown. WT and KO cells are outlined with yellow and magenta dashed line, respectively. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/FUS-Antibody-CL0190-Immunocytochemistry-Immunofluorescence-NBP2-52874-img0021.jpg "<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: FUS Antibody (CL0190) [NBP2-52874] - Immunofluorescence of HeLa wildtype (WT) and FUS knockout (KO) cells labelled with a green or a far-red fluorescent dye, respectively. WT and KO cells were mixed and plated to a 1:1 ratio on coverslips. Cells were stained with Mouse Anti-FUS Monoclonal Antibody (Catalog #NBP2-52874) at 1:1000, followed by the corresponding Alexa Fluor 555-coupled Secondary Antibody. Acquisition of the green (identification of WT cells), red (antibody staining) and far-red (identification of KO cells) channels was performed. Representative image of the red (grayscale) channel is shown. WT and KO cells are outlined with yellow and magenta dashed line, respectively. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).")
![<b>Genetic Strategies Validation. </b>Western Blot: FUS Antibody (CL0190) [NBP2-52874] - Western blot using lysates of HeLa parental cell line (WT) and FUS knockout HeLa cell line (KO), collected in RIPA Buffer. Lysates were prepared using 30 ug of protein and the nitrocellulose membrane was probed with Mouse Anti-FUS Monoclonal Antibody (Catalog #NBP2-52874) at 1:5000 O/N at 4C, followed by peroxidase-conjugated Anti-Mouse Secondary Antibody and ECL detection. A specific band at ~70 kDa was detected in the FUS WT cell line but is not observed in the FUS KO cell line. The Ponceau stained transfer of the blot is shown to confirm equal protein loading. Image, protocol, and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/FUS-Antibody-CL0190-Western-Blot-NBP2-52874-img0023.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: FUS Antibody (CL0190) [NBP2-52874] - Staining in human cerebral cortex and liver tissues. Corresponding FUS RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/FUS-Antibody-CL0190-Immunohistochemistry-Paraffin-NBP2-52874-img0020.jpg)
![<b>Genetic Strategies Validation. </b>Knockdown Validated: FUS Antibody (CL0190) [NBP2-52874] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-FUS monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/FUS-Antibody-CL0190-Western-Blot-NBP2-52874-img0014.jpg)
![Western Blot: FUS Antibody (CL0190) [NBP2-52874] - Lane 1: Marker [kDa], Lane 2: Human cell line HeLa cytoplasmic fraction , Lane 3: Human cell line HeLa membrane fraction , Lane 4: Human cell line HeLa nuclear fraction , Lane 5: Human cell line HeLa chromatin fraction , Lane 6: Human cell line HeLa cytos](http://images.novusbio.com/fullsize/FUS-Antibody-CL0190-Western-Blot-NBP2-52874-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: FUS Antibody (CL0190) [NBP2-52874] - Staining in U2OS cell line with Anti-FUS monoclonal antibody, showing clear nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining shown in green.](http://images.novusbio.com/fullsize/FUS-Antibody-CL0190-Immunocytochemistry-Immunofluorescence-NBP2-52874-img0005.jpg)
![Western Blot: FUS Antibody (CL0190) [NBP2-52874] - Lane 1: Marker [kDa]Lane 2: Human cell line HeLaLane 3: Human cell line A-431Lane 4: Human cell line MCF-7Lane 5: Human cell line U2-OSLane 6: Human cell line Hep-G2](http://images.novusbio.com/fullsize/FUS-Antibody-CL0190-Western-Blot-NBP2-52874-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: FUS Antibody (CL0190) [NBP2-52874] - Staining in A431 cell line with Anti-FUS monoclonal antibody, showing clear nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/FUS-Antibody-CL0190-Immunocytochemistry-Immunofluorescence-NBP2-52874-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: FUS Antibody (CL0190) [NBP2-52874] - Staining in HeLa cell line with Anti-FUS monoclonal antibody, showing clear nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/FUS-Antibody-CL0190-Immunocytochemistry-Immunofluorescence-NBP2-52874-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: FUS Antibody (CL0190) [NBP2-52874] - Staining in MCF7 cell line with Anti-FUS monoclonal antibody, showing clear nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/FUS-Antibody-CL0190-Immunocytochemistry-Immunofluorescence-NBP2-52874-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: FUS Antibody (CL0190) [NBP2-52874] - Staining in U251 cell line with Anti-FUS monoclonal antibody, showing clear nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/FUS-Antibody-CL0190-Immunocytochemistry-Immunofluorescence-NBP2-52874-img0004.jpg)
![Immunohistochemistry-Paraffin: FUS Antibody (CL0190) [NBP2-52874] - Staining of human endometrium shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/FUS-Antibody-CL0190-Immunohistochemistry-Paraffin-NBP2-52874-img0016.jpg)
![Immunohistochemistry-Paraffin: FUS Antibody (CL0190) [NBP2-52874] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.](http://images.novusbio.com/fullsize/FUS-Antibody-CL0190-Immunohistochemistry-Paraffin-NBP2-52874-img0017.jpg)
![Immunohistochemistry-Paraffin: FUS Antibody (CL0190) [NBP2-52874] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/FUS-Antibody-CL0190-Immunohistochemistry-Paraffin-NBP2-52874-img0018.jpg)
![Immunohistochemistry-Paraffin: FUS Antibody (CL0190) [NBP2-52874] - Staining of human liver shows only very weak nuclear positivity in hepatocytes.](http://images.novusbio.com/fullsize/FUS-Antibody-CL0190-Immunohistochemistry-Paraffin-NBP2-52874-img0019.jpg)
![Western Blot: DDX1 Antibody (3E5E2) [NBP2-61745] - Analysis using DDX1 mAb against HEK293 (1) and DDX1 (AA: 642-740)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/DDX1-Antibody-3E5E2-Western-Blot-NBP2-61745-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: DDX1 Antibody (3E5E2) [NBP2-61745] - Analysis of Hela cells using DDX1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/DDX1-Antibody-3E5E2-Immunocytochemistry-Immunofluorescence-NBP2-61745-img0003.jpg)
![Immunohistochemistry-Paraffin: DDX1 Antibody (3E5E2) [NBP2-61745] - Analysis of bladder cancer tissues using DDX1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/DDX1-Antibody-3E5E2-Immunohistochemistry-NBP2-61745-img0005.jpg)
![Flow Cytometry: DDX1 Antibody (3E5E2) [NBP2-61745] - Analysis of Hela cells using DDX1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/DDX1-Antibody-3E5E2-Flow-Cytometry-NBP2-61745-img0002.jpg)
![Western Blot: DDX1 Antibody (3E5E2) [NBP2-61745] - Analysis using DDX1 mAb against human DDX1 (AA: 642-740) recombinant protein. (Expected MW is 37 kDa)](http://images.novusbio.com/fullsize/DDX1-Antibody-3E5E2-Western-Blot-NBP2-61745-img0006.jpg)
![Western Blot: DDX1 Antibody (3E5E2) [NBP2-61745] - Analysis using DDX1 mouse mAb against Hela (1), MCF-7 (2), A431 (3), PC-3 (4), NIH/3T3 (5), Jurkat (6), U251 (7), and HEK293 (8) cell lysate.](http://images.novusbio.com/fullsize/DDX1-Antibody-3E5E2-Western-Blot-NBP2-61745-img0008.jpg)
![Immunohistochemistry-Paraffin: DDX1 Antibody (3E5E2) [NBP2-61745] - Analysis of colon cancer tissues using DDX1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/DDX1-Antibody-3E5E2-Immunohistochemistry-NBP2-61745-img0004.jpg)
![ELISA: DDX1 Antibody (3E5E2) [NBP2-61745] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/DDX1-Antibody-3E5E2-ELISA-NBP2-61745-img0001.jpg)
![Western Blot: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Analysis of TDP43 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: K562 cell lysate Lane 2: Hela cell lysate.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-JM51-10-Western-Blot-NBP2-80137-img0008.jpg "Western Blot: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Analysis of TDP43 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: K562 cell lysate Lane 2: Hela cell lysate.")
![Immunocytochemistry/Immunofluorescence: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Analysis of paraffin-embedded human pancreas tissue usingTDP-43/TARDBP Antibody (JM51-10). Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-JM51-10-Immunocytochemistry-Immunofluorescence-NBP2-80137-img0007.jpg)
![Immunohistochemistry-Paraffin: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-TDP-43/TARDBP antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-JM51-10-Immunohistochemistry-Paraffin-NBP2-80137-img0009.jpg)
![Flow Cytometry: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Analysis of Hela cells with TDP-43/TARDBP Antibody (JM51-10) at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-JM51-10-Flow-Cytometry-NBP2-80137-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Staining TDP-43/TARDBP in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-JM51-10-Immunocytochemistry-Immunofluorescence-NBP2-80137-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Staining TDP-43/TARDBP in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-JM51-10-Immunocytochemistry-Immunofluorescence-NBP2-80137-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Staining TDP-43/TARDBP in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-JM51-10-Immunocytochemistry-Immunofluorescence-NBP2-80137-img0006.jpg)
![Immunohistochemistry-Paraffin: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Analysis of paraffin-embedded mouse placenta tissue using TDP-43/TARDBP Antibody (JM51-10). Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-JM51-10-Immunohistochemistry-Paraffin-NBP2-80137-img0003.jpg)
![Western Blot: FUS Antibody [NB100-2599] - Western blot shows lysates of HEK293 human embryonic kidney parental cell line and FUS knockout (KO) HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human FUS Polyclonal Antibody (Catalog # NB100-2599) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for FUS at approximately 75 kDa (as indicated) in the parental HEK293 celll line, but is not detectable in the knockout HEK293 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/FUS-Antibody-Knockout-Validated-NB100-2599-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: FUS Antibody [NB100-2599] - Western blot shows lysates of HEK293 human embryonic kidney parental cell line and FUS knockout (KO) HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human FUS Polyclonal Antibody (Catalog # NB100-2599) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for FUS at approximately 75 kDa (as indicated) in the parental HEK293 celll line, but is not detectable in the knockout HEK293 cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: FUS Antibody [NB100-2599] - Section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-FUS used at a dilution of 1:100. Detection: DAB](http://images.novusbio.com/fullsize/FUS-Antibody-Immunohistochemistry-Paraffin-NB100-2599-img0007.jpg)
![Immunohistochemistry-Paraffin: FUS Antibody [NB100-2599] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-FUS used at a dilution of 1:500. Detection: DAB staining using anti-rabbit IHC antibody.](http://images.novusbio.com/fullsize/FUS-Antibody-Immunohistochemistry-Paraffin-NB100-2599-img0006.jpg)
![Western Blot: MT-ND4 Antibody [NBP2-47365] - Analysis in human cell line HeLa.](http://images.novusbio.com/fullsize/MT-ND4-Antibody-Western-Blot-NBP2-47365-img0003.jpg "Western Blot: MT-ND4 Antibody [NBP2-47365] - Analysis in human cell line HeLa.")
![Immunohistochemistry-Paraffin: MT-ND4 Antibody [NBP2-47365] - Staining of human Stomach shows strong granular cytoplasmic positivity in enteroendocrine cells.](http://images.novusbio.com/fullsize/MT-ND4-Antibody-Immunohistochemistry-Paraffin-NBP2-47365-img0011.jpg)
![Immunohistochemistry-Paraffin: MT-ND4 Antibody [NBP2-47365] - Staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/MT-ND4-Antibody-Immunohistochemistry-Paraffin-NBP2-47365-img0004.jpg)
![Immunohistochemistry-Paraffin: MT-ND4 Antibody [NBP2-47365] - Staining of human Lymph node shows very weak granular cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/MT-ND4-Antibody-Immunohistochemistry-Paraffin-NBP2-47365-img0009.jpg)
![Immunohistochemistry-Paraffin: MT-ND4 Antibody [NBP2-47365] - Staining of human Salivary gland shows moderate granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/MT-ND4-Antibody-Immunohistochemistry-Paraffin-NBP2-47365-img0010.jpg)
![Western Blot: Calnexin Antibody [NBP1-85519] - LAMP2 released to the cytosol is part of small membrane vesicles. c Sucrose gradient fractions (0.3-2 M) immunoblotted against LAMP2 from cells exposed to LLOMe (1 mM, 2 h). Calnexin is used as an organelle marker. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-020-2527-8), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Western-Blot-NBP1-85519-img0018.jpg "Western Blot: Calnexin Antibody [NBP1-85519] - LAMP2 released to the cytosol is part of small membrane vesicles. c Sucrose gradient fractions (0.3-2 M) immunoblotted against LAMP2 from cells exposed to LLOMe (1 mM, 2 h). Calnexin is used as an organelle marker. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-020-2527-8), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Calnexin Antibody [NBP1-85519] - Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85519-img0006.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-85519] - Staining of human tonsil shows strong cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-Paraffin-NBP1-85519-img0017.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-85519] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-Paraffin-NBP1-85519-img0012.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-85519] - Staining of human cerebral cortex, placenta, testis and tonsil using Anti-Calnexin antibody NBP1-85519 (A) shows similar protein distribution across tissues to independent antibody NBP1-85520 (B).](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-Paraffin-NBP1-85519-img0014.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-85519] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-Paraffin-NBP1-85519-img0015.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-85519] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-Paraffin-NBP1-85519-img0016.jpg)
![Western Blot: VAP-B Antibody [NBP1-89112] - Analysis in human cell lines Caco-2 and HEK293 using anti-VAPB antibody. Corresponding VAPB RNA-seq data are presented for the same cell lines. Loading control: anti-COX4I1.](http://images.novusbio.com/fullsize/VAP-B-Antibody-Western-Blot-NBP1-89112-img0023.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: VAP-B Antibody [NBP1-89112] - Analysis in human cell lines Caco-2 and HEK293 using anti-VAPB antibody. Corresponding VAPB RNA-seq data are presented for the same cell lines. Loading control: anti-COX4I1.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: VAP-B Antibody [NBP1-89112] - Staining of human cell line U-2 OS shows positivity in endoplasmic reticulum. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/VAP-B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89112-img0021.jpg)
![Immunohistochemistry-Paraffin: VAP-B Antibody [NBP1-89112] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/VAP-B-Antibody-Immunohistochemistry-Paraffin-NBP1-89112-img0019.jpg)
![Western Blot: VAP-B Antibody [NBP1-89112] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/VAP-B-Antibody-Western-Blot-NBP1-89112-img0007.jpg)
![Immunohistochemistry-Paraffin: VAP-B Antibody [NBP1-89112] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/VAP-B-Antibody-Immunohistochemistry-Paraffin-NBP1-89112-img0016.jpg)
![Immunohistochemistry-Paraffin: VAP-B Antibody [NBP1-89112] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/VAP-B-Antibody-Immunohistochemistry-Paraffin-NBP1-89112-img0017.jpg)
![Immunohistochemistry-Paraffin: VAP-B Antibody [NBP1-89112] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/VAP-B-Antibody-Immunohistochemistry-Paraffin-NBP1-89112-img0018.jpg)