| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22121SS | |||
| Azide and BSA Free | NBP2-80844 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89236-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-488SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-32932-0.02mg | |||
| Azide and BSA Free | NBP2-34713-0.2mg | |||
| Azide and BSA Free | NBP2-34713-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-22444SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-71656SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-31374SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56351SS | |||
![Western Blot: MBP Antibody (2H9) [NBP2-22121] - Total human brain lysate was separated by SDS-PAGE on a 12% gel and transferred to PVDF membrane. The membrane was then probed with anti-MBP antibody at 2 ug/mL and detected using an anti-mouse HRP labeled secondary antibody and chemiluminescent substrate.](http://images.novusbio.com/fullsize/MBP-Antibody-2H9-Western-Blot-NBP2-22121-img0008.jpg "Western Blot: MBP Antibody (2H9) [NBP2-22121] - Total human brain lysate was separated by SDS-PAGE on a 12% gel and transferred to PVDF membrane. The membrane was then probed with anti-MBP antibody at 2 ug/mL and detected using an anti-mouse HRP labeled secondary antibody and chemiluminescent substrate.")
![Immunocytochemistry/Immunofluorescence: Myelin Basic Protein Antibody (2H9) [NBP2-22121] - Immunofluorescence analysis of MSCS cells using Myelin Basic Protein mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Myelin-Basic-Protein-Antibody-2H9-Immunocytochemistry-Immunofluorescence-NBP2-22121-img0005.jpg)
![Immunohistochemistry-Frozen: MBP Antibody (2H9) [NBP2-22121] - Mouse brain cryosections were stained with anti-MBP (1:400) and anti-mouse Alexa Fluor 555 IgG. Staining specific, hardly any background. Magnification 1:20. Image from verified customer review.](http://images.novusbio.com/fullsize/MBP-Antibody-2H9-Immunohistochemistry-Frozen-NBP2-22121-img0009.jpg)
![Flow Cytometry: Myelin Basic Protein Antibody (2H9) [NBP2-22121] - Flow cytometric analysis of HepG2 cells using Myelin Basic Protein mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Myelin-Basic-Protein-Antibody-2H9-Flow-Cytometry-NBP2-22121-img0004.jpg)
![Immunohistochemistry-Paraffin: Myelin Basic Protein Antibody (2H9) [NBP2-22121] - Immunohistochemical analysis of paraffin-embedded brain tissues using Myelin Basic Protein mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Myelin-Basic-Protein-Antibody-2H9-Immunohistochemistry-Paraffin-NBP2-22121-img0006.jpg)
![Immunohistochemistry-Paraffin: Myelin Basic Protein Antibody (2H9) [NBP2-22121] - Immunohistochemical analysis of paraffin-embedded cerebellum tissues using Myelin Basic Protein mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Myelin-Basic-Protein-Antibody-2H9-Immunohistochemistry-Paraffin-NBP2-22121-img0007.jpg)
![ELISA: Myelin Basic Protein Antibody (2H9) [NBP2-22121] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).](http://images.novusbio.com/fullsize/Myelin-Basic-Protein-Antibody-2H9-ELISA-NBP2-22121-img0001.jpg)
![Immunohistochemistry-Paraffin: ADNP Antibody [NBP1-89236] - Analysis in human testis and liver tissues using NBP1-89236 antibody. Corresponding ADNP RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ADNP-Antibody-Immunohistochemistry-Paraffin-NBP1-89236-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ADNP Antibody [NBP1-89236] - Analysis in human testis and liver tissues using NBP1-89236 antibody. Corresponding ADNP RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: ADNP Antibody [NBP1-89236] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/ADNP-Antibody-Western-Blot-NBP1-89236-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: ADNP Antibody [NBP1-89236] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ADNP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89236-img0009.jpg)
![Immunohistochemistry-Paraffin: ADNP Antibody [NBP1-89236] - Staining of human prostate shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/ADNP-Antibody-Immunohistochemistry-Paraffin-NBP1-89236-img0013.jpg)
![Western Blot: ADNP Antibody [NBP1-89236] - Analysis in human cell line SH-SY5Y.](http://images.novusbio.com/fullsize/ADNP-Antibody-Western-Blot-NBP1-89236-img0007.jpg)
![Immunohistochemistry-Paraffin: ADNP Antibody [NBP1-89236] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/ADNP-Antibody-Immunohistochemistry-Paraffin-NBP1-89236-img0011.jpg)
![Immunohistochemistry-Paraffin: ADNP Antibody [NBP1-89236] - Staining of human endometrium shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/ADNP-Antibody-Immunohistochemistry-Paraffin-NBP1-89236-img0012.jpg)
![Immunohistochemistry-Paraffin: ADNP Antibody [NBP1-89236] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/ADNP-Antibody-Immunohistochemistry-Paraffin-NBP1-89236-img0014.jpg)
![Western Blot: ZIC1 Antibody [NB600-488] - WB analysis of Zic1 in overexpression lysate.](http://images.novusbio.com/fullsize/ZIC1-Antibody-Western-Blot-NB600-488-img0003.jpg "Western Blot: ZIC1 Antibody [NB600-488] - WB analysis of Zic1 in overexpression lysate.")
![Immunohistochemistry-Paraffin: ZIC1 Antibody [NB600-488] - Altered brain pericyte proliferation in Foxc1 mutants. Triple immunofluorescence for Zic1 (pericyte nuclei; green), BrdU (red) and IB4 (blue) highlight proliferating (Zic1+/BrdU+; arrows) pericytes in the E14.5 cerebral vasculature of WT, Foxc1h/h and Foxc1l/l embryos. Image collected and cropped by CiteAb from the following publication (//bio.biologists.org/cgi/doi/10.1242/bio.20135009), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ZIC1-Antibody-Immunohistochemistry-Paraffin-NB600-488-img0005.jpg)
![Western Blot: ZIC1 Antibody [NB600-488] - Western blot analysis of Zic1 in mouse cerebellum extract. Protein extracts were prepared from mouse cerebellum between postnatal day 5 (P5) and P18, as indicated above the lanes.](http://images.novusbio.com/fullsize/ZIC1-Antibody-Western-Blot-NB600-488-img0001.jpg)
![Immunohistochemistry: ZIC1 Antibody [NB600-488] - IHC analysis of Zic1 in mouse kidney using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/ZIC1-Antibody-Immunohistochemistry-NB600-488-img0004.jpg)
![Immunohistochemistry-Paraffin: ZIC1 Antibody [NB600-488] - Pericyte conditional Foxc1 mutants have increased pericyte proliferation and density in the cerebral vasculature. Zic1 (green), BrdU (red), and IB4 (blue) triple immunofluorescence of E14.5 PDGFrbeta-cre; Foxc1fl-fl mutant and PDGFrbeta-cre; Foxc1fl-+ cerebral cortex. Arrows indicate Zic1+/BrdU+ pericytes. Image collected and cropped by CiteAb from the following publication (//bio.biologists.org/cgi/doi/10.1242/bio.20135009), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ZIC1-Antibody-Immunohistochemistry-Paraffin-NB600-488-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: ZIC1 Antibody - BSA Free [NB600-488] - Altered brain pericyte proliferation in Foxc1 mutants.(A–C) PDGFr beta (green) & IB4 (red) immunofluorescence highlight pericyte cell bodies in the cortices of E14.5 WT, Foxc1h/h & Foxc1l/l. (D–F) Triple immunofluorescence for Zic1 (pericyte nuclei; green), BrdU (red) & IB4 (blue) highlight proliferating (Zic1+/BrdU+; arrows) pericytes in the E14.5 cerebral vasculature of WT, Foxc1h/h & Foxc1l/l embryos. (G) Graph depicting quantification of Zic1+ pericyte nuclei/vessel length in all three genotypes at E14.5. (H) Graph depicting quantification of pericyte proliferation (labeling index) at E14.5. Scale bars: 100 µm. Means represent analysis of three independent litters for each mutant genotype (n = 3). Asterisks indicate a statistically significance difference from WT (P<0.05). Error bars depict ±SEM. Image collected & cropped by CiteAb from the following publication (//journals.biologists.com/bio/article/2/7/647/845/Foxc1-is-required-by-pericytes-during-fetal-brain), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-488_rabbit-polyclonal-zic1-antibody-310202416163796.jpg)
![Immunocytochemistry/ Immunofluorescence: ZIC1 Antibody - BSA Free [NB600-488] - Altered brain pericyte proliferation in Foxc1 mutants.(A–C) PDGFr beta (green) & IB4 (red) immunofluorescence highlight pericyte cell bodies in the cortices of E14.5 WT, Foxc1h/h & Foxc1l/l. (D–F) Triple immunofluorescence for Zic1 (pericyte nuclei; green), BrdU (red) & IB4 (blue) highlight proliferating (Zic1+/BrdU+; arrows) pericytes in the E14.5 cerebral vasculature of WT, Foxc1h/h & Foxc1l/l embryos. (G) Graph depicting quantification of Zic1+ pericyte nuclei/vessel length in all three genotypes at E14.5. (H) Graph depicting quantification of pericyte proliferation (labeling index) at E14.5. Scale bars: 100 µm. Means represent analysis of three independent litters for each mutant genotype (n = 3). Asterisks indicate a statistically significance difference from WT (P<0.05). Error bars depict ±SEM. Image collected & cropped by CiteAb from the following publication (//journals.biologists.com/bio/article/2/7/647/845/Foxc1-is-required-by-pericytes-during-fetal-brain), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-488_rabbit-polyclonal-zic1-antibody-310202416175243.jpg)
![Immunocytochemistry/ Immunofluorescence: ZIC1 Antibody - BSA Free [NB600-488] - Pericyte conditional Foxc1 mutants have increased pericyte proliferation & density in the cerebral vasculature.(A,C) Zic1 (green) & IB4 (red) co-immunofluorescence in the cortices of PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ brains at E14.5. (B,D) PDGFr beta (green) & IB4 (red) co-immunofluorescence in the cortices of PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ brains at E14.5. Arrowheads indicate PDGFr beta + pericytes. (E,F) Zic1 (green), BrdU (red), & IB4 (blue) triple immunofluorescence of E14.5 PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ cerebral cortex. Arrows indicate Zic1+/BrdU+ pericytes. (G) Graph depicts quantification of Zic1+ pericyte proliferation (labeling index) in the cerebral vasculature at E14.5. (H,J) Zic1 (green) & IB4 (red) co-immunofluorescence in the cortices of PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ brains at E18.5. (I,K) PDGFr beta (green) & IB4 (red) co-immunofluorescence in the cortices of PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ brains at E18.5. Arrowheads indicate PDGFr beta + pericytes. (L) Graph depicts pericyte density (Zic1+ pericyte nuclei/vessel length) in the cerebral vasculature at E14.5 & E18.5 in PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ animals. Asterisks indicate a statistically significance difference from PDGFr beta -cre; Foxc1fl-+ samples (P<0.05). Analysis of pericyte proliferation & density was performed on control & mutant brains from three separate litters at each time point (n = 3). Error bars depict ±SEM. Scale bars in A,C,E,F,H,J: 100 µm. Scale bars in B,D,I,K: 50 µm. Image collected & cropped by CiteAb from the following publication (//journals.biologists.com/bio/article/2/7/647/845/Foxc1-is-required-by-pericytes-during-fetal-brain), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-488_rabbit-polyclonal-zic1-antibody-310202416171429.jpg)
![Immunocytochemistry/ Immunofluorescence: ZIC1 Antibody - BSA Free [NB600-488] - Pericyte conditional Foxc1 mutants have increased pericyte proliferation & density in the cerebral vasculature.(A,C) Zic1 (green) & IB4 (red) co-immunofluorescence in the cortices of PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ brains at E14.5. (B,D) PDGFr beta (green) & IB4 (red) co-immunofluorescence in the cortices of PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ brains at E14.5. Arrowheads indicate PDGFr beta + pericytes. (E,F) Zic1 (green), BrdU (red), & IB4 (blue) triple immunofluorescence of E14.5 PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ cerebral cortex. Arrows indicate Zic1+/BrdU+ pericytes. (G) Graph depicts quantification of Zic1+ pericyte proliferation (labeling index) in the cerebral vasculature at E14.5. (H,J) Zic1 (green) & IB4 (red) co-immunofluorescence in the cortices of PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ brains at E18.5. (I,K) PDGFr beta (green) & IB4 (red) co-immunofluorescence in the cortices of PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ brains at E18.5. Arrowheads indicate PDGFr beta + pericytes. (L) Graph depicts pericyte density (Zic1+ pericyte nuclei/vessel length) in the cerebral vasculature at E14.5 & E18.5 in PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ animals. Asterisks indicate a statistically significance difference from PDGFr beta -cre; Foxc1fl-+ samples (P<0.05). Analysis of pericyte proliferation & density was performed on control & mutant brains from three separate litters at each time point (n = 3). Error bars depict ±SEM. Scale bars in A,C,E,F,H,J: 100 µm. Scale bars in B,D,I,K: 50 µm. Image collected & cropped by CiteAb from the following publication (//journals.biologists.com/bio/article/2/7/647/845/Foxc1-is-required-by-pericytes-during-fetal-brain), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-488_rabbit-polyclonal-zic1-antibody-310202416161818.jpg)
![Immunocytochemistry/ Immunofluorescence: ZIC1 Antibody - BSA Free [NB600-488] - Pericyte conditional Foxc1 mutants have increased pericyte proliferation & density in the cerebral vasculature.(A,C) Zic1 (green) & IB4 (red) co-immunofluorescence in the cortices of PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ brains at E14.5. (B,D) PDGFr beta (green) & IB4 (red) co-immunofluorescence in the cortices of PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ brains at E14.5. Arrowheads indicate PDGFr beta + pericytes. (E,F) Zic1 (green), BrdU (red), & IB4 (blue) triple immunofluorescence of E14.5 PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ cerebral cortex. Arrows indicate Zic1+/BrdU+ pericytes. (G) Graph depicts quantification of Zic1+ pericyte proliferation (labeling index) in the cerebral vasculature at E14.5. (H,J) Zic1 (green) & IB4 (red) co-immunofluorescence in the cortices of PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ brains at E18.5. (I,K) PDGFr beta (green) & IB4 (red) co-immunofluorescence in the cortices of PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ brains at E18.5. Arrowheads indicate PDGFr beta + pericytes. (L) Graph depicts pericyte density (Zic1+ pericyte nuclei/vessel length) in the cerebral vasculature at E14.5 & E18.5 in PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ animals. Asterisks indicate a statistically significance difference from PDGFr beta -cre; Foxc1fl-+ samples (P<0.05). Analysis of pericyte proliferation & density was performed on control & mutant brains from three separate litters at each time point (n = 3). Error bars depict ±SEM. Scale bars in A,C,E,F,H,J: 100 µm. Scale bars in B,D,I,K: 50 µm. Image collected & cropped by CiteAb from the following publication (//journals.biologists.com/bio/article/2/7/647/845/Foxc1-is-required-by-pericytes-during-fetal-brain), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-488_rabbit-polyclonal-zic1-antibody-310202416171415.jpg)
![Immunocytochemistry/ Immunofluorescence: ZIC1 Antibody - BSA Free [NB600-488] - Pericyte conditional Foxc1 mutants have increased pericyte proliferation & density in the cerebral vasculature.(A,C) Zic1 (green) & IB4 (red) co-immunofluorescence in the cortices of PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ brains at E14.5. (B,D) PDGFr beta (green) & IB4 (red) co-immunofluorescence in the cortices of PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ brains at E14.5. Arrowheads indicate PDGFr beta + pericytes. (E,F) Zic1 (green), BrdU (red), & IB4 (blue) triple immunofluorescence of E14.5 PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ cerebral cortex. Arrows indicate Zic1+/BrdU+ pericytes. (G) Graph depicts quantification of Zic1+ pericyte proliferation (labeling index) in the cerebral vasculature at E14.5. (H,J) Zic1 (green) & IB4 (red) co-immunofluorescence in the cortices of PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ brains at E18.5. (I,K) PDGFr beta (green) & IB4 (red) co-immunofluorescence in the cortices of PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ brains at E18.5. Arrowheads indicate PDGFr beta + pericytes. (L) Graph depicts pericyte density (Zic1+ pericyte nuclei/vessel length) in the cerebral vasculature at E14.5 & E18.5 in PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ animals. Asterisks indicate a statistically significance difference from PDGFr beta -cre; Foxc1fl-+ samples (P<0.05). Analysis of pericyte proliferation & density was performed on control & mutant brains from three separate litters at each time point (n = 3). Error bars depict ±SEM. Scale bars in A,C,E,F,H,J: 100 µm. Scale bars in B,D,I,K: 50 µm. Image collected & cropped by CiteAb from the following publication (//journals.biologists.com/bio/article/2/7/647/845/Foxc1-is-required-by-pericytes-during-fetal-brain), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-488_rabbit-polyclonal-zic1-antibody-31020241616049.jpg)
![Immunocytochemistry/ Immunofluorescence: ZIC1 Antibody - BSA Free [NB600-488] - Pericyte conditional Foxc1 mutants have increased pericyte proliferation & density in the cerebral vasculature.(A,C) Zic1 (green) & IB4 (red) co-immunofluorescence in the cortices of PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ brains at E14.5. (B,D) PDGFr beta (green) & IB4 (red) co-immunofluorescence in the cortices of PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ brains at E14.5. Arrowheads indicate PDGFr beta + pericytes. (E,F) Zic1 (green), BrdU (red), & IB4 (blue) triple immunofluorescence of E14.5 PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ cerebral cortex. Arrows indicate Zic1+/BrdU+ pericytes. (G) Graph depicts quantification of Zic1+ pericyte proliferation (labeling index) in the cerebral vasculature at E14.5. (H,J) Zic1 (green) & IB4 (red) co-immunofluorescence in the cortices of PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ brains at E18.5. (I,K) PDGFr beta (green) & IB4 (red) co-immunofluorescence in the cortices of PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ brains at E18.5. Arrowheads indicate PDGFr beta + pericytes. (L) Graph depicts pericyte density (Zic1+ pericyte nuclei/vessel length) in the cerebral vasculature at E14.5 & E18.5 in PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ animals. Asterisks indicate a statistically significance difference from PDGFr beta -cre; Foxc1fl-+ samples (P<0.05). Analysis of pericyte proliferation & density was performed on control & mutant brains from three separate litters at each time point (n = 3). Error bars depict ±SEM. Scale bars in A,C,E,F,H,J: 100 µm. Scale bars in B,D,I,K: 50 µm. Image collected & cropped by CiteAb from the following publication (//journals.biologists.com/bio/article/2/7/647/845/Foxc1-is-required-by-pericytes-during-fetal-brain), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-488_rabbit-polyclonal-zic1-antibody-310202416154640.jpg)
![Immunocytochemistry/ Immunofluorescence: ZIC1 Antibody - BSA Free [NB600-488] - Pericyte conditional Foxc1 mutants have increased pericyte proliferation & density in the cerebral vasculature.(A,C) Zic1 (green) & IB4 (red) co-immunofluorescence in the cortices of PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ brains at E14.5. (B,D) PDGFr beta (green) & IB4 (red) co-immunofluorescence in the cortices of PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ brains at E14.5. Arrowheads indicate PDGFr beta + pericytes. (E,F) Zic1 (green), BrdU (red), & IB4 (blue) triple immunofluorescence of E14.5 PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ cerebral cortex. Arrows indicate Zic1+/BrdU+ pericytes. (G) Graph depicts quantification of Zic1+ pericyte proliferation (labeling index) in the cerebral vasculature at E14.5. (H,J) Zic1 (green) & IB4 (red) co-immunofluorescence in the cortices of PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ brains at E18.5. (I,K) PDGFr beta (green) & IB4 (red) co-immunofluorescence in the cortices of PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ brains at E18.5. Arrowheads indicate PDGFr beta + pericytes. (L) Graph depicts pericyte density (Zic1+ pericyte nuclei/vessel length) in the cerebral vasculature at E14.5 & E18.5 in PDGFr beta -cre; Foxc1fl-fl mutant & PDGFr beta -cre; Foxc1fl-+ animals. Asterisks indicate a statistically significance difference from PDGFr beta -cre; Foxc1fl-+ samples (P<0.05). Analysis of pericyte proliferation & density was performed on control & mutant brains from three separate litters at each time point (n = 3). Error bars depict ±SEM. Scale bars in A,C,E,F,H,J: 100 µm. Scale bars in B,D,I,K: 50 µm. Image collected & cropped by CiteAb from the following publication (//journals.biologists.com/bio/article/2/7/647/845/Foxc1-is-required-by-pericytes-during-fetal-brain), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-488_rabbit-polyclonal-zic1-antibody-310202416182612.jpg)
![Immunocytochemistry/ Immunofluorescence: ZIC1 Antibody - BSA Free [NB600-488] - Altered brain pericyte proliferation in Foxc1 mutants.(A–C) PDGFr beta (green) & IB4 (red) immunofluorescence highlight pericyte cell bodies in the cortices of E14.5 WT, Foxc1h/h & Foxc1l/l. (D–F) Triple immunofluorescence for Zic1 (pericyte nuclei; green), BrdU (red) & IB4 (blue) highlight proliferating (Zic1+/BrdU+; arrows) pericytes in the E14.5 cerebral vasculature of WT, Foxc1h/h & Foxc1l/l embryos. (G) Graph depicting quantification of Zic1+ pericyte nuclei/vessel length in all three genotypes at E14.5. (H) Graph depicting quantification of pericyte proliferation (labeling index) at E14.5. Scale bars: 100 µm. Means represent analysis of three independent litters for each mutant genotype (n = 3). Asterisks indicate a statistically significance difference from WT (P<0.05). Error bars depict ±SEM. Image collected & cropped by CiteAb from the following publication (//journals.biologists.com/bio/article/2/7/647/845/Foxc1-is-required-by-pericytes-during-fetal-brain), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-488_rabbit-polyclonal-zic1-antibody-31020241616060.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (QBEnd/10) [NBP2-32932] - Formalin-fixed, paraffin-embedded human tonsil (10X) stained with CD34 MAb (QBEnd/10)](http://images.novusbio.com/fullsize/CD34-Antibody-QBEnd-10-Immunohistochemistry-Paraffin-NBP2-32932-img0001.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (QBEnd/10) [NBP2-32932] - Formalin-paraffin human tonsil (20X) stained with CD34 MAb (QBEnd/10)](http://images.novusbio.com/fullsize/CD34-Antibody-QBEnd-10-Immunohistochemistry-Paraffin-NBP2-32932-img0002.jpg)
![Immunohistochemistry: POMC Antibody [NB100-1533] - Representative confocal images of POMC in POMC-transfected WT and Sel1L-/- N2a cells. White arrows point to POMC-containing secretory granules, while yellow arrows point to perinuclear POMC. KDEL marks the ER. Representative data from at least 2 independent experiments are shown. Image collected and cropped by CiteAb from the following publication (jci.org/articles/view/96420), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/POMC-Antibody-Immunohistochemistry-NB100-1533-img0008.jpg "Immunohistochemistry: POMC Antibody [NB100-1533] - Representative confocal images of POMC in POMC-transfected WT and Sel1L-/- N2a cells. White arrows point to POMC-containing secretory granules, while yellow arrows point to perinuclear POMC. KDEL marks the ER. Representative data from at least 2 independent experiments are shown. Image collected and cropped by CiteAb from the following publication (jci.org/articles/view/96420), licensed under a CC-BY license.")
![Flow Cytometry: POMC Antibody [NB100-1533] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/POMC-Antibody-Flow-Cytometry-NB100-1533-img0006.jpg)
![Immunohistochemistry-Paraffin: POMC Antibody [NB100-1533] - Staining of paraffin embedded Human Pituitary Gland. Antibody at 2 ug/mL. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.](http://images.novusbio.com/fullsize/POMC-Antibody-Immunohistochemistry-Paraffin-NB100-1533-img0003.jpg)
![Immunohistochemistry: POMC Antibody [NB100-1533] - Representative immunofluorescence images of (B) POMC as a prohormone (n = 5-6 each) in the ARC of mice approximately 5 to 10 weeks of age. Image collected and cropped by CiteAb from the following publication (jci.org/articles/view/96420), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/POMC-Antibody-Immunohistochemistry-NB100-1533-img0007.jpg)
![Immunocytochemistry/ Immunofluorescence: POMC Antibody [NB100-1533] - Pathogenic POMC-C28F mutation is completely rescued by an intragenic suppressor mutation C50S.(A) Schematic diagram showing the sequence & positions of Cys residues in POMC-WT, C28F, & C28F/C50S. (B) Western blot analyses of POMC-Flag immunoprecipitates in HEK293T cells transfected with a combination of Myc- or Flag-tagged POMC under nonreducing (– beta -ME) & reducing (+ beta -ME) SDS-PAGE. Two panels were from the same experiment at the same exposure time, with the irrelevant lanes in the middle cut off. (C) Sucrose gradient fractionation & Western blot analysis of HEK293T cells expressing POMC-C28F or -C28F/C50S under nonreducing (– beta -ME) & reducing (+ beta -ME) SDS-PAGE. (D) Metabolic labeling experiments to visualize the maturation of nascent POMC in HEK293T cells transfected with POMC-Myc. Cells were pulsed for 30 minutes & chased for the indicated times, followed by immunoprecipitation with anti-Myc agarose beads & separation on SDS-PAGE gels under nonreducing or reducing conditions & autoradiography. (E) Representative confocal images of POMC in POMC-transfected WT N2a cells. White arrows point to secreted POMC in granules, while yellow arrows point to perinuclear POMC, possibly in the form of aggregates. KDEL marks the ER. Representative data from at least 2 independent experiments shown. Image collected & cropped by CiteAb from the following publication (//www.jci.org/articles/view/96420), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1533_goat-polyclonal-pomc-antibody-310202415535174.jpg)
![Western Blot: GABA-A R delta Antibody [NB300-200] - mouse whole brain (1) and mouse synaptic plasma membrane (2) lysates showing specific immunolabeling of the Approx. 50 kDa GAmma-subunit of the GABAA-R.](http://images.novusbio.com/fullsize/GABA-A-R-delta-Antibody-Western-Blot-NB300-200-img0001.jpg "Western Blot: GABA-A R delta Antibody [NB300-200] - mouse whole brain (1) and mouse synaptic plasma membrane (2) lysates showing specific immunolabeling of the Approx. 50 kDa GAmma-subunit of the GABAA-R.")
![Western Blot: Cav3.2 Antibody (S55/10) [NBP1-22444] - Western Blot analysis of Rat brain membrane lysate (native) showing detection of ~261 kDa Cav3.2protein using Mouse Anti-Cav3.2Monoclonal Antibody, Clone N55/10 (NBP1-22444). Block: 2% Skim Milk + 2% BSA in TBST. Primary Antibody: Mouse Anti-Cav3.2Monoclonal Antibody (NBP1-22444) at 1:1000 for 2 hours at RT. Secondary Antibody: Anti-Mouse: HRP at 1:4000. Predicted/Observed Size: ~261 kDa.](http://images.novusbio.com/fullsize/Cav3-2-Antibody-S55-10-Western-Blot-NBP1-22444-img0014.jpg "Western Blot: Cav3.2 Antibody (S55/10) [NBP1-22444] - Western Blot analysis of Rat brain membrane lysate (native) showing detection of ~261 kDa Cav3.2protein using Mouse Anti-Cav3.2Monoclonal Antibody, Clone N55/10 (NBP1-22444). Block: 2% Skim Milk + 2% BSA in TBST. Primary Antibody: Mouse Anti-Cav3.2Monoclonal Antibody (NBP1-22444) at 1:1000 for 2 hours at RT. Secondary Antibody: Anti-Mouse: HRP at 1:4000. Predicted/Observed Size: ~261 kDa.")
![Immunohistochemistry-Paraffin: Cav3.2 Antibody (S55/10) [NBP1-22444] - Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaV3.2 Calcium Channel Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/Cav3-2-Antibody-S55-10-Immunohistochemistry-Paraffin-NBP1-22444-img0011.jpg)
![Western Blot: Cav3.2 Antibody (S55/10) [NBP1-22444] - Western Blot using rat brain membranes and NBP1-22444 to target Cav3.2 channels visible at about 260 kDa.](http://images.novusbio.com/fullsize/Cav3-2-Antibody-S55-10-Western-Blot-NBP1-22444-img0012.jpg)
![Western Blot: Cav3.2 Antibody (S55/10) [NBP1-22444] - Western blot analysis of CaV3.2 proteins in mesenteric arteries of young versus old mice. (h), quantification of Western blot results. Mesenteric arteries were taken from 9 mice in each group. *, p < .05. Image collected and cropped by CiteAb from the following publication (onlinelibrary.wiley.com/doi/abs/10.1111/acel.13134) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Cav3-2-Antibody-S55-10-Western-Blot-NBP1-22444-img0013.jpg)
![Immunohistochemistry-Frozen: Cav3.2 Antibody (S55/10) [NBP1-22444] - Tissue: frozen brain section. Species: human. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-CaV3.2 Calcium channel Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 uL for 5 minutes at RT.](http://images.novusbio.com/fullsize/Cav3-2-Antibody-S55-10-Immunohistochemistry-Frozen-NBP1-22444-img0010.jpg)
![Western Blot: Cav3.2 Antibody (S55/10) [NBP1-22444] - Age attenuates the role of CaV3.2 channels in Ca2+ spark generation & decreases CaV3.2 protein expression in VSMC. (a), Ca2+ fluorescence images of a Fluo‐4‐AM–loaded VSMC from a young mouse & time course of Ca2+ fluorescence changes in the cellular ROI (upper panel). Cell boundary is marked with dashed line. (b), same as (a) but in the presence of Ni2+ (50 µM). (c), same as (a) but in a VSMC from an old mouse. (d), same as (c) but in the presence of Ni2+ (50 µM). (e, f), summary of the results. Ca2+ spark frequency (e) & fraction of cells producing Ca2+ sparks (f) in VSMCs from young mice (n = 102), in VSMCs from young mice cells incubated with Ni2+ (n = 85), in VSMCs from aged mice (n = 129), & in VSMCs from aged mice cells incubated with Ni2+ (n = 127). Cells were isolated from 4 mice in each group; 25–40 cells were recorded & analyzed from each mouse. VSMC, vascular smooth muscle cell. (g), Western blot analysis of CaV3.2 proteins in mesenteric arteries of young versus old mice. (h), quantification of Western blot results. Mesenteric arteries were taken from 9 mice in each group. *, p < .05. n.s., not significant Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32187825), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-22444_mouse-monoclonal-cav3-2-antibody-s55-10-31020241617145.jpg)
![Western Blot: GAPDH Antibody [NB300-324] - Detection of Human and Mouse GAPDH by Western Blot. Theoretical molecular weight: 36 kDa. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GAPDH antibody NB300-324 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-324-img0009.jpg "Western Blot: GAPDH Antibody [NB300-324] - Detection of Human and Mouse GAPDH by Western Blot. Theoretical molecular weight: 36 kDa. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GAPDH antibody NB300-324 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunocytochemistry/Immunofluorescence: GAPDH Antibody [NB300-324] - GAPDH antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/GAPDH-Antibody-Immunocytochemistry-Immunofluorescence-NB300-324-img0002.jpg)
![Immunohistochemistry: GAPDH Antibody [NB300-324] - Detection of human GAPDH by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-GAPDH (NB100-79808). Detection: DAB](http://images.novusbio.com/fullsize/GAPDH-Antibody-Immunohistochemistry-NB300-324-img0010.jpg)
![Western Blot: GAPDH Antibody [NB300-324] - Detection of Human and Mouse GAPDH, theoretical molecular weight 36 kDa. Whole cell lysate from HeLa (5, 15 and 50 mcg) and mouse NIH3T3 (M; 50 mcg). Affinity purified rabbit anti-GAPDH antibody used at 0.04 mcg/ml. Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-324-img0008.jpg)
![Immunohistochemistry-Paraffin: GAPDH Antibody [NB300-324] - IHC detection of GAPDH in formalin-fixed paraffin-embedded human lung carcinoma tissue sections with NB300-324 at a dilution of 1:200](http://images.novusbio.com/fullsize/GAPDH-Antibody-Immunohistochemistry-Paraffin-NB300-324-img0003.jpg)
![Immunohistochemistry-Paraffin: GAPDH Antibody [NB300-324] - IHC detection of GAPDH in formalin-fixed paraffin-embedded mouse squamous cell carcinoma tissue sections with NB300-324 at a dilution of 1:200.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Immunohistochemistry-Paraffin-NB300-324-img0004.jpg)
![Simple Western: GAPDH Antibody [NB300-324] - Simple Western lane view shows a specific band for GAPDH in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Note: band observed higher than predicted molecular weight of 36 kDa.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Simple-Western-NB300-324-img0007.jpg)
![Western Blot: GPER/GPR30 Antibody [NBP1-31239] - The MW of GPR30 is estimated to be 42 kDa, but higher MW sizes have been reported due to glycosylation and interaction with other proteins. Bands are identified as glycosylated and nonglycosylated based on reports cited in the text, but could include interaction with other proteins. For each GPER western, the membrane was stripped and reprobed for b-actin as a loading control. Quantitation of GPER was evaluated by summing all immunoreactive bands and dividing by b-actin, then normalizing to HBEC2-KT in each blot. Image collected and cropped by CiteAb from the following publication (biomedcentral.com/1471-2407/12/624), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GPER-GPR30-Antibody-Western-Blot-NBP1-31239-img0012.jpg "Western Blot: GPER/GPR30 Antibody [NBP1-31239] - The MW of GPR30 is estimated to be 42 kDa, but higher MW sizes have been reported due to glycosylation and interaction with other proteins. Bands are identified as glycosylated and nonglycosylated based on reports cited in the text, but could include interaction with other proteins. For each GPER western, the membrane was stripped and reprobed for b-actin as a loading control. Quantitation of GPER was evaluated by summing all immunoreactive bands and dividing by b-actin, then normalizing to HBEC2-KT in each blot. Image collected and cropped by CiteAb from the following publication (biomedcentral.com/1471-2407/12/624), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: GPER/GPR30 Antibody [NBP1-31239] - Detection of GPER/GPR30 in HUVEC nucleus. Image courtesy of a product review by Dr. Subhadeep Chakrabarti of University of Alberta.](http://images.novusbio.com/fullsize/GPER-GPR30-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31239-img0010.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: GPER/GPR30 Antibody [NBP1-31239] - A. 50 ug 293T whole cell lysate/extract. B. 50 ug whole cell lysate/extract of GFP-human GPR30-transfected 293T cells (No boiling). C. 50 ug whole cell lysate/extract of GFP-human GPR30 and GPR30 siRNA-transfected 293T cells (No boiling). 5 % SDS-PAGE GPR30 antibody ) dilution: 1:1000](http://images.novusbio.com/fullsize/GPER-GPR30-Antibody-Western-Blot-NBP1-31239-img0011.jpg)
![Western Blot: GPER/GPR30 Antibody [NBP1-31239] - AhR & GPR30 receptors are present & functional in the MCF10AT1 cells. (A) RT-qPCR analysis of AhR & GPR30 mRNA expression levels represented in arbitrary units (a.u.) in the MCF10AT1 & MCF10CA1a.cl1 cells. MCF-7 cells were used as a control. Values represent mean ± SD of three independent experiments conducted in triplicate. (B) Representative Western blot analyses from three independent experiments of AhR & GPR30 protein expression in MCF10AT1 & MCF10CA1a.cl1 cells. MCF-7 cells were used as a control. (C) XRE-luciferase activity following 8 h exposure of MCF10AT1 cells to ITE at the indicated concentrations. TCDD 10−7 M was used as a control & results were expressed as % of TCDD 10−7 M activity. ***p < 0.001 in Student t-test. (D) XRE-luciferase activity upon 8 h of exposure to ITE 10−10 M alone or in combination with GNF351 at the indicated concentrations. TCDD 10−7 M was used as a control, & results were expressed as % of TCDD 10−7 M activity. Student t-tests revealed the statistically significant differences between unexposed & exposed cells: ***p < 0.001; & between ITE & ITE+GNF351: ###p < 0.001. Values in (C,D) represent mean ± SD of three independent experiments. (E) Representative Western blot analyses from three independent experiments of the phospho-MAPK/MAPK ratio upon exposure of MCF10AT1 cells to G1 (GPR30 agonist) for the times indicated, in the presence or absence of a 2 h pre-treatment with G15 (GPR30 antagonist). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32670863), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-31239_rabbit-polyclonal-gper-gpr30-antibody-310202416171440.jpg)
![Western Blot: GPER/GPR30 Antibody [NBP1-31239] - Effects of short-term exposure of BPA, B[a]P, ITE & G1 10−10 M on AIG & MFE are inhibited by siRNA-AhR & siRNA-GPR30. Representative Western blot analysis from three independent experiments of AhR & GPR30 expression in transfected MCF10AT1 cells with (A) siRNA-AhR, (B) siRNA-GPR30 or their scrambled controls. Quantification of protein expression levels was normalized against tubulin expression. (C,D) Secondary mammospheres formation & (E,F) average number of colonies in soft agar, with the following treatments: BPA and/or B[a]P, G1, or ITE, 10−10 M. Cells were transfected with either siRNA-AhR, siRNA-GPR30 or their scrambled controls before being subjected to the treatments. Treatments were maintained throughout the course of experiments. (mean ± SD of 2 independent experiments, in triplicate). ***p < 0.001, *p < 0.05 vs. their respective unexposed; ###p < 0.001 siRNA vs. scrambled in Student t-test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32670863), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-31239_rabbit-polyclonal-gper-gpr30-antibody-310202416173515.jpg)
![Western Blot: GPER/GPR30 Antibody [NBP1-31239] - Human endothelial cells express GPR30 protein in the cell nucleus.(A) Confluent monolayers of HUVECs from 3 different cords were lysed & the protein lysates were immunoblotted for GPR30. alpha -tubulin was used as loading control. (B) HUVEC monolayers at 30–40% confluence were treated with 40 nM siRNA (control or GPR30_4) for 48 hours prior to lysis followed by immunoblotting of the cell lysates for GPR30. alpha -tubulin was used as loading control. Data shown are mean ± SEM of 4 independent experiments. ** & ## indicate p<0.01 compared to untreated & control siRNA-treated cells, respectively. (C) Confluent HUVECs grown on glass coverslips were fixed, permeabilized & immunostained with anti-GPR30 antibody. Nuclei were stained with Hoechst33342 dye. The merged image shows GPR30 (red) & nuclei (blue) in pseudocolor. Representative images from 3 independent experiments are shown. Bar, 20 µm. (D) Confluent HUVECs were lysed & fractionated into cytosolic (C) & nuclear (N) fractions prior to western blotting for eNOS, GPR30, p65, alpha -tubulin & c-Jun. A representative set of images (obtained from different membranes) from 3 independent experiments is shown. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23285008), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-31239_rabbit-polyclonal-gper-gpr30-antibody-310202416175294.jpg)
![Western Blot: GPER/GPR30 Antibody [NBP1-31239] - Effects of short-term exposure of BPA, B[a]P, ITE & G1 10−10 M on AIG & MFE are inhibited by siRNA-AhR & siRNA-GPR30. Representative Western blot analysis from three independent experiments of AhR & GPR30 expression in transfected MCF10AT1 cells with (A) siRNA-AhR, (B) siRNA-GPR30 or their scrambled controls. Quantification of protein expression levels was normalized against tubulin expression. (C,D) Secondary mammospheres formation & (E,F) average number of colonies in soft agar, with the following treatments: BPA and/or B[a]P, G1, or ITE, 10−10 M. Cells were transfected with either siRNA-AhR, siRNA-GPR30 or their scrambled controls before being subjected to the treatments. Treatments were maintained throughout the course of experiments. (mean ± SD of 2 independent experiments, in triplicate). ***p < 0.001, *p < 0.05 vs. their respective unexposed; ###p < 0.001 siRNA vs. scrambled in Student t-test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32670863), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-31239_rabbit-polyclonal-gper-gpr30-antibody-310202416173516.jpg)
![Western Blot: GPER/GPR30 Antibody [NBP1-31239] - Effects of short-term exposure of BPA, B[a]P, ITE & G1 10−10 M on AIG & MFE are inhibited by siRNA-AhR & siRNA-GPR30. Representative Western blot analysis from three independent experiments of AhR & GPR30 expression in transfected MCF10AT1 cells with (A) siRNA-AhR, (B) siRNA-GPR30 or their scrambled controls. Quantification of protein expression levels was normalized against tubulin expression. (C,D) Secondary mammospheres formation & (E,F) average number of colonies in soft agar, with the following treatments: BPA and/or B[a]P, G1, or ITE, 10−10 M. Cells were transfected with either siRNA-AhR, siRNA-GPR30 or their scrambled controls before being subjected to the treatments. Treatments were maintained throughout the course of experiments. (mean ± SD of 2 independent experiments, in triplicate). ***p < 0.001, *p < 0.05 vs. their respective unexposed; ###p < 0.001 siRNA vs. scrambled in Student t-test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32670863), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-31239_rabbit-polyclonal-gper-gpr30-antibody-310202416163721.jpg)
![Western Blot: OPA1 Antibody (1E8-1D9) [NBP1-71656] - Analysis of OPA1 expression in 1) HeLa 2) MEF 3) HepG2 4) A431 5) CHO 6)PC12 and 7) Ntera2 whole cell lysates using NBP1-71656.](http://images.novusbio.com/fullsize/OPA1-Antibody-1E8-1D9-Western-Blot-NBP1-71656-img0009.jpg "Western Blot: OPA1 Antibody (1E8-1D9) [NBP1-71656] - Analysis of OPA1 expression in 1) HeLa 2) MEF 3) HepG2 4) A431 5) CHO 6)PC12 and 7) Ntera2 whole cell lysates using NBP1-71656.")
![Immunocytochemistry/Immunofluorescence: OPA1 Antibody (1E8-1D9) [NBP1-71656] - OPA1 antibody was tested in ARPE-19 cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/OPA1-Antibody-1E8-1D9-Immunocytochemistry-Immunofluorescence-NBP1-71656-img0007.jpg)
![Immunohistochemistry: OPA1 Antibody (1E8-1D9) [NBP1-71656] - Analysis of OPA1 on mouse skin using NBP1-71656.](http://images.novusbio.com/fullsize/OPA1-Antibody-1E8-1D9-Immunohistochemistry-NBP1-71656-img0008.jpg)
![Simple Western: OPA1 Antibody (1E8-1D9) [NBP1-71656] - Image shows a specific band for OPA1 in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/OPA1-Antibody-1E8-1D9-Simple-Western-NBP1-71656-img0010.jpg)
![Simple Western: OPA1 Antibody (1E8-1D9) - BSA Free [NBP1-71656] - Image shows a specific band for OPA1 in 0.1 ug/uL mouse hippocampus tissue lysate. Primary antibody dilution: 1:200. Image from verified customer review.](http://images.novusbio.com/fullsize/OPA1-Antibody-1E8-1D9-BSA-Free-Simple-Western-NBP1-71656-img0011.jpg)
![Western Blot: CCR5 Antibody [NBP2-31374] - Image of anti-CCR5 antibody. Whole cell protein from Daudi (1), K562 (2) and THP-1 (3) cell lines were separated by SDS-PAGE and protein transfered to PVDF. The membrane was probed with anti-CCR5 antibody at 2 ug/mL and detected with an anti-rabbit-HRP secondary antibody and chemiluminescence. CCR5 is shown at 40 kDa.](http://images.novusbio.com/fullsize/CCR5-Antibody-Western-Blot-NBP2-31374-img0003.jpg "Western Blot: CCR5 Antibody [NBP2-31374] - Image of anti-CCR5 antibody. Whole cell protein from Daudi (1), K562 (2) and THP-1 (3) cell lines were separated by SDS-PAGE and protein transfered to PVDF. The membrane was probed with anti-CCR5 antibody at 2 ug/mL and detected with an anti-rabbit-HRP secondary antibody and chemiluminescence. CCR5 is shown at 40 kDa.")
![Immunohistochemistry-Paraffin: CCR5 Antibody [NBP2-31374] - Detection of CCR5 and virus on brain tissue. CCR5 expression was analyzed using Immunohistochemistry on the layer III of frontal cortex from macaques that were uninfected (A) and untreated controls, and Meth-only treated (B). Pictures were at 16x magnification. Sites of interest (rectangles) were further magnified. Image collected and cropped by CiteAb from the following publication (//bmcimmunol.biomedcentral.com/articles/10.1186/s12865-016-0145-0), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CCR5-Antibody-Immunohistochemistry-Paraffin-NBP2-31374-img0007.jpg)
![Western Blot: CCR5 Antibody [NBP2-31374] - Detection of CCR5 partial recombinant protein by using CCR5 antibody at a concentration of 0.5 ug/mL.](http://images.novusbio.com/fullsize/CCR5-Antibody-Western-Blot-NBP2-31374-img0001.jpg)
![Immunohistochemistry-Paraffin: CCR5 Antibody [NBP2-31374] - Analysis of CCR5 protein in a section of malignant stromal tumor of small bowel from human using CCR5 antibody at a concentration of 5 ug/mL.](http://images.novusbio.com/fullsize/CCR5-Antibody-Immunohistochemistry-Paraffin-NBP2-31374-img0002.jpg)
![Immunohistochemistry-Paraffin: CCR5 Antibody [NBP2-31374] - Staining in a Rhesus macaque brain. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CCR5-Antibody-Immunohistochemistry-Paraffin-NBP2-31374-img0005.jpg)
![Immunohistochemistry-Paraffin: CCR5 Antibody [NBP2-31374] - Human skin section. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CCR5-Antibody-Immunohistochemistry-Paraffin-NBP2-31374-img0006.jpg)
![Simple Western: CCR5 Antibody [NBP2-31374] - Lane view shows a specific band for CCR5 in 0.5 mg/mL of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/CCR5-Antibody-Simple-Western-NBP2-31374-img0004.jpg)
![Immunohistochemistry: CCR5 Antibody [NBP2-31374] - Detection of CCR5 & virus on brain tissue. CCR5 expression was analyzed using Immunohistochemistry on the layer III of frontal cortex from macaques that were a Uninfected & untreated controls, b Meth-only treated, c SIV-only infected, & d SIV-infected & Meth-treated. e SIV-infected & Meth-treated. Pictures were at 16× magnification. Sites of interest (rectangles) were further magnified. f Brain viral load (Student’s t test) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27107567), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-31374_rabbit-polyclonal-ccr5-antibody-310202415345331.jpg)
![Immunohistochemistry: CCR5 Antibody [NBP2-31374] - Detection of CCR5 & virus on brain tissue. CCR5 expression was analyzed using Immunohistochemistry on the layer III of frontal cortex from macaques that were a Uninfected & untreated controls, b Meth-only treated, c SIV-only infected, & d SIV-infected & Meth-treated. e SIV-infected & Meth-treated. Pictures were at 16× magnification. Sites of interest (rectangles) were further magnified. f Brain viral load (Student’s t test) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27107567), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-31374_rabbit-polyclonal-ccr5-antibody-310202415371940.jpg)
![Immunohistochemistry: CCR5 Antibody [NBP2-31374] - Detection of CCR5 & virus on brain tissue. CCR5 expression was analyzed using Immunohistochemistry on the layer III of frontal cortex from macaques that were a Uninfected & untreated controls, b Meth-only treated, c SIV-only infected, & d SIV-infected & Meth-treated. e SIV-infected & Meth-treated. Pictures were at 16× magnification. Sites of interest (rectangles) were further magnified. f Brain viral load (Student’s t test) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27107567), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-31374_rabbit-polyclonal-ccr5-antibody-310202415291975.jpg)
![Immunohistochemistry: CCR5 Antibody [NBP2-31374] - Detection of CCR5 & virus on brain tissue. CCR5 expression was analyzed using Immunohistochemistry on the layer III of frontal cortex from macaques that were a Uninfected & untreated controls, b Meth-only treated, c SIV-only infected, & d SIV-infected & Meth-treated. e SIV-infected & Meth-treated. Pictures were at 16× magnification. Sites of interest (rectangles) were further magnified. f Brain viral load (Student’s t test) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27107567), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-31374_rabbit-polyclonal-ccr5-antibody-31020241517527.jpg)
![Immunohistochemistry: CCR5 Antibody [NBP2-31374] - Detection of CCR5 & virus on brain tissue. CCR5 expression was analyzed using Immunohistochemistry on the layer III of frontal cortex from macaques that were a Uninfected & untreated controls, b Meth-only treated, c SIV-only infected, & d SIV-infected & Meth-treated. e SIV-infected & Meth-treated. Pictures were at 16× magnification. Sites of interest (rectangles) were further magnified. f Brain viral load (Student’s t test) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27107567), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-31374_rabbit-polyclonal-ccr5-antibody-310202415304245.jpg)
![Western Blot: 5-HT3A Antibody [NB100-56351] - The expression of 5-HT3A receptor increased in the E2 + FS group compared to that in the oil + non-FS group. *P < 0.05 versus the oil + non-FS group. E2: estradiol; FS: forced swim. Image collected and cropped by CiteAb from the following publication (//journals.sagepub.com/doi/10.1177/1744806919859723), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/5-HT3A-Antibody-Western-Blot-NB100-56351-img0013.jpg "<b>Biological Strategies Validation. </b>Western Blot: 5-HT3A Antibody [NB100-56351] - The expression of 5-HT3A receptor increased in the E2 + FS group compared to that in the oil + non-FS group. *P < 0.05 versus the oil + non-FS group. E2: estradiol; FS: forced swim. Image collected and cropped by CiteAb from the following publication (//journals.sagepub.com/doi/10.1177/1744806919859723), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: 5-HT3A Antibody [NB100-56351] - Analysisof 5-HT3A in human testis using 5-HT3A antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/5-HT3A-Antibody-Immunohistochemistry-Paraffin-NB100-56351-img0010.jpg)
![Western Blot: 5-HT3A Antibody [NB100-56351] - Analysis of 5-HT3A in A: human, B: mouse and C: rat brain tissue lysate using 5-HT3A antibody at 3 ug/mL.](http://images.novusbio.com/fullsize/5-HT3A-Antibody-Western-Blot-NB100-56351-img0012.jpg)
![Simple Western: 5-HT3A Antibody [NB100-56351] - Image shows a specific band for 5HT3A in 0.5 mg/mL of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/5-HT3A-Antibody-Simple-Western-NB100-56351-img0007.jpg)
