Neuroscience reagents: Novus Biologicals

NB100-82086

RelA/NFkB p65 [p Ser276] Antibody

(1 Review)

(6 Publications)

0.05 ml

NB100-82086

Immunohistochemistry-Paraffin: RelA/NFkB p65 [p Ser276] Antibody [NB100-82086] - Human breast carcinoma tissue.

Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NB100-82086] - Western Blot analysis of various cells using NB100-82086 diluted at 1:1000

Immunohistochemistry-Paraffin: RelA/NFkB p65 [p Ser276] Antibody [NB100-82086] - Analysis of paraffin- embedded human breast carcinoma tissue using NB100-82086 incubated with blocking peptide (right)

Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 [p Ser276] Antibody [NB100-82086] - Immunofluorescence staining of methanol-fixed MEF cells untreated or treated with TNF using NB100-82086

Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NB100-82086] - Lane 1: HEK293. Lane 2: Lentivirus mediated NFkB overexpression. We image submitted by a verified customer review.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP1-89766

Glutaminase Antibody - BSA Free

(5 Publications)

0.1 ml

NBP1-89766

Immunohistochemistry-Paraffin: Glutaminase Antibody [NBP1-89766] - Staining of human cerebral cortex shows moderate to strong granular cytoplasmic positivity in neurons.

Immunohistochemistry-Paraffin: Glutaminase Antibody [NBP1-89766] - Staining of human duodenum shows moderate granular cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: Glutaminase Antibody [NBP1-89766] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.

Immunohistochemistry-Paraffin: Glutaminase Antibody [NBP1-89766] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

<b>Biological Strategies Validation. </b>Glutaminase-Antibody-Western-Blot-NBP1-89766-img0016.jpg

Western Blot: Glutaminase Antibody [NBP1-89766] - Region-based expression of the pyruvate recycling pathway marker protein glutaminase (GLT) the VMN of icv Lz-pretreated male & female rats. GLT protein was measured by Western blot in the rostral (a; F(7,40) = 12.20; p < 0.0001), middle (b; F(7,40) = 17.39; p < 0.0001), & caudal (c; F(7,40) = 11.67; p < 0.0001) VMN of V/V, V/INS, Lz/V, & Lz/INS groups of male (M; left-hand side) & female (F; right-hand side) rats. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. For each VMN segment, data depict mean normalized GAD O.D. values ± S.E.M. Results are summarized in the table at bottom; ↑ & ↓ denote a relative increase or decrease, respectively, between treatment groups; no change (NC) between groups is also indicated Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33238883), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NLS1183

GPER/GPR30 Antibody - BSA Free

(2 Reviews)

(6 Publications)

0.05 ml

NLS1183

Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS1183] - Analysis of anti-GPER1 / GPR30 antibody with human, pancreas, islet.

Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS1183] - Analysis of anti-GPER1 / GPR30 antibody with human adrenal.

Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS1183] - Human Skin, Melanoma formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS1183] - Anti-GPER1 / GPR30 antibody IHC of human colon, carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS1183] - Anti-GPER1 / GPR30 antibody IHC of human Lung, Small Cell Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS1183] - Brain, Parkinson's Lewy Body

Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS1183] - Brain, cerebellum, Purkinje neuron

Reactivity:

Hu, Mu, Pm

Species Glossary

Reactivity: Hu, Mu, Pm

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP1-77679

IGF-I R/IGF1R Antibody - BSA Free

(3 Publications)

0.1 ml

NBP1-77679

Immunohistochemistry-Paraffin: IGF-I R Antibody [NBP1-77679] - IHC analysis of a formalin fixed paraffin embedded tissue section of the human kidney cancer xenograft using 1:200 dilution of IFG-I R antibody (NBP1-77679). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin.

Simple Western: IGF-I R Antibody [NBP1-77679] - Simple Western lane view shows a specific band for IGF-1 R in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Reactivity:

Hu, Mu, Rt, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Pm

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-77679AF350
Alexa Fluor 405	NBP1-77679AF405
Alexa Fluor 488	NBP1-77679AF488
Alexa Fluor 532	NBP1-77679AF532
Alexa Fluor 594	NBP1-77679AF594
Alexa Fluor 647	NBP1-77679AF647
Alexa Fluor 700	NBP1-77679AF700
Alexa Fluor 750	NBP1-77679AF750
Allophycocyanin	NBP1-77679APC
Biotin	NBP1-77679B
CoraFluor 1	NBP1-77679CL1
DyLight 350	NBP1-77679UV
DyLight 405	NBP1-77679V
DyLight 488	NBP1-77679G
DyLight 550	NBP1-77679R
DyLight 594	NBP1-77679DL594
DyLight 650	NBP1-77679C
DyLight 680	NBP1-77679FR
DyLight 755	NBP1-77679IR
FITC	NBP1-77679F
HRP	NBP1-77679H
Janelia Fluor 525	NBP1-77679JF525
Janelia Fluor 549	NBP1-77679JF549
Janelia Fluor 646	NBP1-77679JF646
Janelia Fluor 669	NBP1-77679JF669
PE	NBP1-77679PE
PerCP	NBP1-77679PCP
mFluor Violet 450 SE	NBP1-77679MFV450
mFluor Violet 500 SE	NBP1-77679MFV500
mFluor Violet 610 SE	NBP1-77679MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-77679SS

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NBP2-22139

Sonic Hedgehog/Shh Antibody - BSA Free

(5 Publications)

0.1 ml

NBP2-22139

Immunohistochemistry: Sonic Hedgehog Antibody [NBP2-22139] - IHC analysis of Sonic Hedgehog in mouse kidney.

Reactivity:

Hu, Mu, Rt, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Pm

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-22139AF350
Alexa Fluor 405	NBP2-22139AF405
Alexa Fluor 488	NBP2-22139AF488
Alexa Fluor 532	NBP2-22139AF532
Alexa Fluor 594	NBP2-22139AF594
Alexa Fluor 647	NBP2-22139AF647
Alexa Fluor 700	NBP2-22139AF700
Alexa Fluor 750	NBP2-22139AF750
Allophycocyanin	NBP2-22139APC
Biotin	NBP2-22139B
CoraFluor 1	NBP2-22139CL1
DyLight 350	NBP2-22139UV
DyLight 405	NBP2-22139V
DyLight 488	NBP2-22139G
DyLight 550	NBP2-22139R
DyLight 594	NBP2-22139DL594
DyLight 650	NBP2-22139C
DyLight 680	NBP2-22139FR
DyLight 755	NBP2-22139IR
FITC	NBP2-22139F
HRP	NBP2-22139H
Janelia Fluor 525	NBP2-22139JF525
Janelia Fluor 549	NBP2-22139JF549
Janelia Fluor 646	NBP2-22139JF646
Janelia Fluor 669	NBP2-22139JF669
PE	NBP2-22139PE
PerCP	NBP2-22139PCP
mFluor Violet 450 SE	NBP2-22139MFV450
mFluor Violet 500 SE	NBP2-22139MFV500
mFluor Violet 610 SE	NBP2-22139MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-22139SS

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NB300-204

HIP1 Antibody (1B11) - BSA Free

(1 Review)

(7 Publications)

0.1 ml

NB300-204

Reactivity:

Hu, Mu, Ba, Rt(-)

Species Glossary

Reactivity: Hu, Mu, Ba, Rt(-)

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB300-204SS

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NB100-2381

VEGF Antibody - BSA Free

(7 Publications)

0.1 ml

NB100-2381

HIF-1 alpha protein and HIF-1 alpha target genes are elevated in SCs following retrovirus administration. Expression of mRNA for VP16, HIF-1 alpha , and beta -actin in transfected SCs was confirmed by PCR in VP16 and VP16-HIF SCs (A). Protein expression of VP16, HIF-1 alpha , HIF-2 alpha , VEGF, enolase, and protein loading controls (GFP or beta -actin) in transfected SCs was assessed by western blotting (B). Protein expression was normalized to the loading control. Relative intensity of VP16 expression is shown in C, and the fold change in protein expression relative to control SCs is shown for HIF-1 alpha (D) HIF-2 alpha (E), VEGF (F), and enolase (G). Values are as follows: VP16 (control: 0.0 +/- 0.0; VP16: 0.40 +/- 0.01; VP16-HIF: 0.41 +/- 0.00); HIF-1 alpha (control: 1.0 +/- 0.04; VP16: 1.0 +/- 0.04; VP16-HIF: 5.9 +/- 0.01); HIF-2 alpha (control: 1.0 +/- 0.01; VP16: 1.0 +/- 0.01; VP16-HIF: 1.0 +/- 0.02); VEGF (control: 1.0 +/- 0.01; VP16: 1.02 +/- 0.02; VP16-HIF: 2.2 +/- 0.01); enolase (control: 1.0 +/- 0.01; VP16: 1.0 +/- 0.04; VP16-HIF: 1.7 +/- 0.00); n = 3/group. Mean +/- SEM, *p < 0.0001. Figure Contributions: Veena Kandaswamy generated the VP16-HIF cells. Veena Kandaswamy performed the PCRs. Ying Dai performed the western blottings. Ying Dai and Caitlin Hill analyzed the data. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31488552), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Bv, Ca, Ch, Eq, Gp

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Ca, Ch, Eq, Gp

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-2381AF350
Alexa Fluor 405	NB100-2381AF405
Alexa Fluor 488	NB100-2381AF488
Alexa Fluor 532	NB100-2381AF532
Alexa Fluor 594	NB100-2381AF594
Alexa Fluor 647	NB100-2381AF647
Alexa Fluor 700	NB100-2381AF700
Alexa Fluor 750	NB100-2381AF750
Allophycocyanin	NB100-2381APC
Biotin	NB100-2381B
CoraFluor 1	NB100-2381CL1
DyLight 350	NB100-2381UV
DyLight 405	NB100-2381V
DyLight 488	NB100-2381G
DyLight 550	NB100-2381R
DyLight 594	NB100-2381DL594
DyLight 650	NB100-2381C
DyLight 680	NB100-2381FR
DyLight 755	NB100-2381IR
FITC	NB100-2381F
HRP	NB100-2381H
Janelia Fluor 525	NB100-2381JF525
Janelia Fluor 549	NB100-2381JF549
Janelia Fluor 646	NB100-2381JF646
Janelia Fluor 669	NB100-2381JF669
PE	NB100-2381PE
PerCP	NB100-2381PCP
mFluor Violet 450 SE	NB100-2381MFV450
mFluor Violet 500 SE	NB100-2381MFV500
mFluor Violet 610 SE	NB100-2381MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-2381SS

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NBP1-28635

Dtk/TYRO3 Antibody - BSA Free

(5 Publications)

0.1 ml

NBP1-28635

Flow Cytometry: Dtk/TYRO3 Antibody [NBP1-28635] - An intracellular stain was performed on HeLa cells with Dtk/TYRO3 Antibody NBP1-28635AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Flow (Intracellular): Dtk/TYRO3 Antibody [NBP1-28635] - An intracellular stain was performed on U-937 cells with Dtk/TYRO3 Antibody NBP1-28635AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-28635AF350
Alexa Fluor 405	NBP1-28635AF405
Alexa Fluor 488	NBP1-28635AF488
Alexa Fluor 532	NBP1-28635AF532
Alexa Fluor 594	NBP1-28635AF594
Alexa Fluor 647	NBP1-28635AF647
Alexa Fluor 700	NBP1-28635AF700
Alexa Fluor 750	NBP1-28635AF750
Allophycocyanin	NBP1-28635APC
Allophycocyanin/Cy7	NBP1-28635APCCY7
Biotin	NBP1-28635B
CoraFluor 1	NBP1-28635CL1
DyLight 350	NBP1-28635UV
DyLight 405	NBP1-28635V
DyLight 488	NBP1-28635G
DyLight 550	NBP1-28635R
DyLight 594	NBP1-28635DL594
DyLight 650	NBP1-28635C
DyLight 680	NBP1-28635FR
DyLight 755	NBP1-28635IR
FITC	NBP1-28635F
HRP	NBP1-28635H
Janelia Fluor 525	NBP1-28635JF525
Janelia Fluor 549	NBP1-28635JF549
Janelia Fluor 646	NBP1-28635JF646
Janelia Fluor 669	NBP1-28635JF669
PE	NBP1-28635PE
PE/Atto594	NBP1-28635PEATT594
PE/Cy5.5	NBP1-28635PECY55
PE/Cy7	NBP1-28635PECY7
PerCP	NBP1-28635PCP
mFluor Violet 450 SE	NBP1-28635MFV450
mFluor Violet 500 SE	NBP1-28635MFV500
mFluor Violet 610 SE	NBP1-28635MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-28635SS

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NB300-186

IGSF4A/SynCAM1/CADM1 Antibody - BSA Free

(2 Publications)

0.1 ml

NB300-186

$Western Blot: IGSF4A/SynCAM1/CADM1 Antibody [NB300-186] - Rat brain and HEK cell lysates showing different glycosylation patterns. Brain fractions give banding patterns of a core glycosylation population around 50kDa and a slightly higher glycosylated population around 65 kDa. The heterologous HEK cell lysates give a single, fully glycosylated, band around 107kDa.$

Simple Western: IGSF4A/SynCAM1/CADM1 Antibody [NB300-186] - Simple Western lane view shows a specific band for SynCAM in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

$Effect of SH, CN and ESI protocol on synaptic structural markers expression in the crude membrane fraction of the mPFC of PND-75 male (top) and female (bottom) rats. Protein levels of PSD95 (A), SynCAM 1 (B) in male rats and PSD95 (C), SynCAM 1 (D) in female rats are expressed as percentages of SH-CTRL and represent the mean +/- S.E.M. of six rats per group (SynCAM: 1 outlier in CN-CTRL males). Representative immunoblots (bottom) are shown for each protein in the respective panels (E, F). *p<0.05 vs. SH-CTRL, $p<0.05, $$p<0.01, $$$p<0.001 vs CN+CTRL (Two-way ANOVA followed by Tukey’s multiple comparisons test). SH, Standard Housing; CN, Communal Nesting; CTRL, controls no ESI; ESI, Early Social Isolation. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/38835543), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

$Effect of SH, CN and ESI protocol on synaptic structural markers expression in the crude membrane fraction of the mPFC of PND35 male (top) and female (middle) rats. Protein levels of PSD95 (A), SynCAM 1 (B) in male rats and PSD95 (C), SynCAM 1 (D) in female rats are expressed as percentages of SH-CTRL and represent the mean +/- S.E.M. of six rats per group. Representative immunoblots (bottom) are shown for each protein in the respective panels (E, F). *p<0.05, **p<0.01, ***p<0.001 vs. SH-CTRL, $p<0.05, $$p<0.01, $$$p<0.001 vs CN+CTRL (Two-way ANOVA followed by Tukey’s multiple comparisons test). SH, Standard Housing; CN, Communal Nesting; CTRL, controls no ESI; ESI, Early Social Isolation. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/38835543), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

$Effect of SH, CN and ESI protocol on synaptic structural markers expression in the crude membrane fraction of the mPFC of PND-75 male (top) and female (bottom) rats. Protein levels of PSD95 (A), SynCAM 1 (B) in male rats and PSD95 (C), SynCAM 1 (D) in female rats are expressed as percentages of SH-CTRL and represent the mean +/- S.E.M. of six rats per group (SynCAM: 1 outlier in CN-CTRL males). Representative immunoblots (bottom) are shown for each protein in the respective panels (E, F). *p<0.05 vs. SH-CTRL, $p<0.05, $$p<0.01, $$$p<0.001 vs CN+CTRL (Two-way ANOVA followed by Tukey’s multiple comparisons test). SH, Standard Housing; CN, Communal Nesting; CTRL, controls no ESI; ESI, Early Social Isolation. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/38835543), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Reactivity:

Hu, Rt

Species Glossary

Reactivity: Hu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB300-186SS

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NBP2-02577

Profilin 1 Antibody (OTI1D5)

(3 Publications)

0.1 ml

NBP2-02577

Immunohistochemistry-Paraffin: Profilin 1 Antibody (1D5) [NBP2-02577] - Staining of paraffin-embedded Human pancreas tissue using anti-Profilin 1 mouse monoclonal antibody.

Flow Cytometry: Profilin 1 Antibody (1D5) [NBP2-02577] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Profilin 1 antibody, and then analyzed by flow cytometry.

Western Blot: Profilin 1 Antibody (1D5) [NBP2-02577] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Profilin 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Profilin 1.

Western Blot: Profilin 1 Antibody (1D5) [NBP2-02577] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Profilin 1 monoclonal antibody.

Western Blot: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - Analysis of extracts (10ug) from 10 Human tissue by using anti-PFN1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

Immunohistochemistry-Paraffin: Profilin 1 Antibody (1D5) [NBP2-02577] - Staining of paraffin-embedded Human Kidney tissue using anti-Profilin 1 mouse monoclonal antibody.

Flow Cytometry: Profilin 1 Antibody (1D5) [NBP2-02577] - Analysis of Hela cells, using anti-Profilin 1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry: Profilin 1 Antibody (1D5) [NBP2-02577] - Analysis of Jurkat cells, using anti-Profilin 1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

$Immunoprecipitation: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - RASSF1A depletion does not affect nuclear F‐actin levelsWestern blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with truncated RASSF1A mutants.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRASSF1A & siRASSF1A together with XPO6 plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRNA‐resistant FLAG‐RASSF1A plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siMST2. Quantification of nuclear actin & profilin relative to Lamin A/C is shown. Error bars derive from three independent experiments & represent the SEM.Western blot analysis of exportin‐6 (XPO6) & importin‐9 (IPO9) in the absence of RASSF1A. GAPDH was used as a loading control.Confocal images of endogenous filamentous actin (F‐actin) in siCTRL & siRASSF1A cells using phalloidin staining (Alexa Fluor 568‐conjugated, red). DNA was stained with DAPI. Scale bars = 10 μm.Western blot analysis of actin, profilin, GAPDH & Lamin A/C levels in nuclear & cytoplasmic fractions of MDA‐MB‐231 cells treated with DMSO or 5′‐aza‐dC. The graph shows the nuclear levels of actin & profilin relative to Lamin A/C in MDA‐MB‐231 cells expressing RASSF1A. Error bars derive from two independent experiments & represent the SEM.Data information: Two‐tailed Student's t‐test was used for statistical analysis.*P < 0.05, **P < 0.01, ***P < 0.001.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31414556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

$Immunoprecipitation: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - RASSF1A is involved in actin & profilin nuclear export processHeLa cells treated with control or RASSF1A siRNA were fractionated into cytoplasmic & nuclear extracts. Lysates from each fraction were probed for actin & profilin alongside GAPDH (as a marker of the cytoplasmic fraction) & Lamin A/C (as a marker of the nuclear fraction). Right: quantification of nuclear actin & profilin relative to Lamin A/C is shown. Error bars derive from two independent experiments & represent the SEM.Immunofluorescence images of profilin in control & RASSF1A siRNA‐transfected HeLa cells. Right: the profilin localisation was scored as nuclear/cytoplasmic or predominantly cytoplasmic in approximately 100 cells. Error bars derive from three independent experiments & represent the SEM.Confocal images of endogenous monomeric globular actin (G‐actin) in siCTRL & siRASSF1A cells using DNase I staining (Alexa Fluor 488‐conjugated, green). Scale bars = 10 μm.Western blot analysis of actin, profilin, GAPDH & Lamin A/C levels in nuclear & cytoplasmic fractions of MDA‐MB‐231 cells treated with control pcDNA3 or RASSF1A vector. The graph shows the nuclear levels of actin & profilin relative to Lamin A/C in MDA‐MB‐231 cells expressing RASSF1A. Error bars derive from two independent experiments & represent the SEM.Data information: Two‐tailed Student's t‐test was used for statistical analysis. *P < 0.05, **P < 0.01.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31414556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

$Immunoprecipitation: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - RASSF1A depletion does not affect nuclear F‐actin levelsWestern blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with truncated RASSF1A mutants.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRASSF1A & siRASSF1A together with XPO6 plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRNA‐resistant FLAG‐RASSF1A plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siMST2. Quantification of nuclear actin & profilin relative to Lamin A/C is shown. Error bars derive from three independent experiments & represent the SEM.Western blot analysis of exportin‐6 (XPO6) & importin‐9 (IPO9) in the absence of RASSF1A. GAPDH was used as a loading control.Confocal images of endogenous filamentous actin (F‐actin) in siCTRL & siRASSF1A cells using phalloidin staining (Alexa Fluor 568‐conjugated, red). DNA was stained with DAPI. Scale bars = 10 μm.Western blot analysis of actin, profilin, GAPDH & Lamin A/C levels in nuclear & cytoplasmic fractions of MDA‐MB‐231 cells treated with DMSO or 5′‐aza‐dC. The graph shows the nuclear levels of actin & profilin relative to Lamin A/C in MDA‐MB‐231 cells expressing RASSF1A. Error bars derive from two independent experiments & represent the SEM.Data information: Two‐tailed Student's t‐test was used for statistical analysis.*P < 0.05, **P < 0.01, ***P < 0.001.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31414556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Reactivity:

Hu, Mu, Rt, Ca, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Ca, Pm

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-73605AF350
Alexa Fluor 405	NBP2-73605AF405
Alexa Fluor 488	NBP2-73605AF488
Alexa Fluor 532	NBP2-73605AF532
Alexa Fluor 594	NBP2-73605AF594
Alexa Fluor 647	NBP2-73605AF647
Alexa Fluor 700	NBP2-73605AF700
Alexa Fluor 750	NBP2-73605AF750
Allophycocyanin	NBP2-73605APC
Biotin	NBP2-73605B
CoraFluor 1	NBP2-73605CL1
DyLight 350	NBP2-73605UV
DyLight 405	NBP2-73605V
DyLight 488	NBP2-73605G
DyLight 550	NBP2-73605R
DyLight 594	NBP2-73605DL594
DyLight 650	NBP2-73605C
DyLight 680	NBP2-73605FR
DyLight 755	NBP2-73605IR
FITC	NBP2-73605F
HRP	NBP2-73605H
Janelia Fluor 525	NBP2-73605JF525
Janelia Fluor 549	NBP2-73605JF549
Janelia Fluor 646	NBP2-73605JF646
Janelia Fluor 669	NBP2-73605JF669
PE	NBP2-73605PE
PerCP	NBP2-73605PCP
mFluor Violet 450 SE	NBP2-73605MFV450
mFluor Violet 500 SE	NBP2-73605MFV500
mFluor Violet 610 SE	NBP2-73605MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-73605

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NBP2-60735

LC3B Antibody (1251B) - BSA Free

0.1 mg

NBP2-60735

Immunocytochemistry/Immunofluorescence: LC3B Antibody (1251B) [NBP2-60735] - HeLa cells were treated with 50uM CQ (Chloroquine) overnight, fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti- [Catalog # NBP2-60735] at 2 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

<b>Biological Strategies Validation. </b>Western Blot: LC3B Antibody (1251B) [NBP2-60735] - Total protein from HeLa and Neuro2A cells treated with or without 50 uM chloroquine (CQ) for 24 hours was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF (Polyvinylidene difluoride) membrane and blocked in 5% non-fat milk in TBST (Tris-buffered saline, 0.1% Tween 20). The membrane was probed with 12.0 ug/ml anti-LC3B Antibody (1251B) [Catalog # NBP2-60735] in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Immunohistochemistry-Paraffin: LC3B Antibody (1251B) [NBP2-60735] - Analysis of a FFPE (Formalin-Fixed Paraffin-Embedded) tissue section of mouse brain using anti-LC3B antibody (clone 1251B) [Catalog # NBP2-60735] at 5ug/ml concentration (1:200 dilution). The primary antibody binding to LC3 in cells was detected using HRP conjugated anti-Rabbit secondary antibody with DAB (3,3'Diaminobenzidine) reagent, and the sections were further counterstained with hematoxylin for labeling cellular nuclei. This LC3 antibody generated a diffused staining in all cell types (except the endothelial cells of blood vessels) and the signal was strongest in a subset of neuronal cells. A few cells depicted LC3 puncta formation/dotted staining in apparently autophagic cells.

<b>Biological Strategies Validation. </b>Flow (Intracellular): LC3B Antibody (1251B) [NBP2-60735] - HeLa cells were either (A) untreated or (B) treated with 50uM chloroquine for 24 hours. An intracellular stain was performed with anti-LC3B (1251B) antibody [Catalog # NBP2-60735] (blue) and a matched isotype control [Catalog # MAB1050] (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by rabbit IgG APC-conjugated secondary antibody (F0111, R&D Systems).

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-60735AF350
Alexa Fluor 405	NBP2-60735AF405
Alexa Fluor 488	NBP2-60735AF488
Alexa Fluor 532	NBP2-60735AF532
Alexa Fluor 594	NBP2-60735AF594
Alexa Fluor 647	NBP2-60735AF647
Alexa Fluor 700	NBP2-60735AF700
Alexa Fluor 750	NBP2-60735AF750
Allophycocyanin	NBP2-60735APC
Biotin	NBP2-60735B
CoraFluor 1	NBP2-60735CL1
DyLight 350	NBP2-60735UV
DyLight 405	NBP2-60735V
DyLight 488	NBP2-60735G
DyLight 550	NBP2-60735R
DyLight 594	NBP2-60735DL594
DyLight 650	NBP2-60735C
DyLight 680	NBP2-60735FR
DyLight 755	NBP2-60735IR
FITC	NBP2-60735F
HRP	NBP2-60735H
Janelia Fluor 525	NBP2-60735JF525
Janelia Fluor 549	NBP2-60735JF549
Janelia Fluor 646	NBP2-60735JF646
Janelia Fluor 669	NBP2-60735JF669
PE	NBP2-60735PE
PerCP	NBP2-60735PCP
mFluor Violet 450 SE	NBP2-60735MFV450
mFluor Violet 500 SE	NBP2-60735MFV500
mFluor Violet 610 SE	NBP2-60735MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-60735SS
Azide and BSA Free	NBP2-80831

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NB300-325

GAPDH Antibody

(2 Reviews)

(4 Publications)

0.1 ml

NB300-325

Western Blot: GAPDH Antibody [NB300-325] - Whole cell lysate (50 ug) from HeLa (H) and 293T (50 ug) probed with GAPDH Antibody diluted at 0.04 ug/ml

Western Blot: GAPDH Antibody [NB300-325] - Detection of Mouse GAPDH Whole cell lysate (5, 15 and 50 ug) from mouse NIH3T3 cells. Another Affinity purified rabbit anti-GAPDH used at 0.04 mcg/ml. Chemiluminescence with an exposure time of 30 seconds.

Simple Western: GAPDH Antibody [NB300-325] - Simple Western lane view shows a specific band for GAPDH in 0.05 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Western Blot: GAPDH Antibody [NB300-325] - GCNY-medicated serum decreased Nrf2 levels & inflammatory cytokines of NF-kappa B & IL-6. (A) Intracellular relative ROS levels in HMC-3 cells treated with different concentrations (0, 50, 100, & 200 μm) of H2O2 for 4 h. Auto-fluo refers to the auto-fluorescence intensity value of HMC-3 cells. (B,C) The mRNA & protein levels of Nrf2 were expressed by HMC-3 treated with different concentrations of H2O2. (D) The significantly elevated whole protein level in NC + H2O2 group cells indicates an increased proliferation of HMC-3 cells compared to the NC group cells & (E) ROS levels were attenuated in cells treated by GCNY-medicated serum. (F–H) Through automated Western blot analysis, GCNY group cells expressed lower levels of Nrf2, NF-kappa s p65, pNF-kappa B p65 (ser536), & IL-6 compared with NC + H2O2 group cells. ns, no significance, *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/36188472), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Marker

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Kit Type

Lysate Type

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

RelA/NFkB p65 [p Ser276] Antibody

Glutaminase Antibody - BSA Free

GPER/GPR30 Antibody - BSA Free

IGF-I R/IGF1R Antibody - BSA Free

Sonic Hedgehog/Shh Antibody - BSA Free

HIP1 Antibody (1B11) - BSA Free

VEGF Antibody - BSA Free

Dtk/TYRO3 Antibody - BSA Free

IGSF4A/SynCAM1/CADM1 Antibody - BSA Free

Profilin 1 Antibody (OTI1D5)

LC3B Antibody (1251B) - BSA Free

GAPDH Antibody