| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71134 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71225 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-74571SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-92327SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-75477SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89468-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44421 | |||
| BSA Free | NBP2-42388-25ul | |||
![Western Blot: LIM1 Antibody (OTI2D5) [NBP2-01926] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIM1.](http://images.novusbio.com/fullsize/LIM1-Antibody-2D5-Western-Blot-NBP2-01926-img0022.jpg "Western Blot: LIM1 Antibody (OTI2D5) [NBP2-01926] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIM1.")
![Immunocytochemistry/Immunofluorescence: LIM1 Antibody (OTI2D5) [NBP2-01926] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY LIM1.](http://images.novusbio.com/fullsize/LIM1-Antibody-2D5-Immunocytochemistry-NBP2-01926-img0024.jpg)
![Immunohistochemistry-Paraffin: LIM1 Antibody (OTI2D5) [NBP2-01926] - Staining of paraffin-embedded Human prostate tissue using anti-LIM1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/LIM1-Antibody-2D5-Immunohistochemistry-Paraffin-NBP2-01926-img0021.jpg)
![Flow Cytometry: LIM1 Antibody (OTI2D5) [NBP2-01926] - Analysis of Jurkat cells, using anti-LHX1 antibody (Red) compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/LIM1-Antibody-OTI2D5-Flow-Cytometry-NBP2-01926-img0025.jpg)
![Immunohistochemistry-Paraffin: LIM1 Antibody (OTI2D5) [NBP2-01926] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-LIM1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/LIM1-Antibody-2D5-Immunohistochemistry-Paraffin-NBP2-01926-img0012.jpg)
![Immunohistochemistry-Paraffin: LIM1 Antibody (OTI2D5) [NBP2-01926] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-LIM1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/LIM1-Antibody-2D5-Immunohistochemistry-Paraffin-NBP2-01926-img0019.jpg)
![Immunohistochemistry-Paraffin: LIM1 Antibody (OTI2D5) [NBP2-01926] - Staining of paraffin-embedded Human Kidney tissue using anti-LIM1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/LIM1-Antibody-2D5-Immunohistochemistry-Paraffin-NBP2-01926-img0014.jpg)
![Immunohistochemistry-Paraffin: LIM1 Antibody (OTI2D5) [NBP2-01926] - Staining of paraffin-embedded Human liver tissue using anti-LIM1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/LIM1-Antibody-2D5-Immunohistochemistry-Paraffin-NBP2-01926-img0015.jpg)
![Immunohistochemistry-Paraffin: LIM1 Antibody (OTI2D5) [NBP2-01926] - Staining of paraffin-embedded Human pancreas tissue using anti-LIM1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/LIM1-Antibody-2D5-Immunohistochemistry-Paraffin-NBP2-01926-img0020.jpg)
![Flow Cytometry: LIM1 Antibody (OTI2D5) [NBP2-01926] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LIM1 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/LIM1-Antibody-2D5-Flow-Cytometry-NBP2-01926-img0023.jpg)
![Western Blot: OCT4 Antibody (OTI9B7) [NBP1-47923] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OCT4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OCT4](http://images.novusbio.com/fullsize/OCT4-Antibody-OTI9B7-Western-Blot-NBP1-47923-img0007.jpg "Western Blot: OCT4 Antibody (OTI9B7) [NBP1-47923] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OCT4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OCT4")
![Immunocytochemistry/Immunofluorescence: OCT4 Antibody (OTI9B7) [NBP1-47923] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY 41186.](http://images.novusbio.com/fullsize/OCT4-Antibody-OTI9B7-Immunocytochemistry-Immunofluorescence-NBP1-47923-img0006.jpg)
![Immunohistochemistry-Paraffin: OCT4 Antibody (OTI9B7) [NBP1-47923] - Staining of paraffin-embedded Human Ovary tissue using anti-41186 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/OCT4-Antibody-OTI9B7-Immunohistochemistry-Paraffin-NBP1-47923-img0004.jpg)
![Western Blot: OCT4 Antibody (OTI9B7) [NBP1-47923] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OCT4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OCT4.](http://images.novusbio.com/fullsize/OCT4-Antibody-OTI9B7-Western-Blot-NBP1-47923-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: OCT4 Antibody (OTI9B7) [NBP1-47923] - Representative IF IHC-stained sections of MDBMSCC. Expression of both SOX2 (red) and OCT4 (green), appearing as orange, was seen on cells within the tumor nests and the stroma, and the endothelium of the microvessels within the stroma. Scale bars: 20 um. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fsurg.2016.00046), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/OCT4-Antibody-OTI9B7-Immunocytochemistry-Immunofluorescence-NBP1-47923-img0008.jpg)
![Immunohistochemistry-Paraffin: OCT4 Antibody (OTI9B7) [NBP1-47923] - Staining of paraffin-embedded Human endometrium tissue using anti-41186 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/OCT4-Antibody-OTI9B7-Immunohistochemistry-Paraffin-NBP1-47923-img0002.jpg)
![Immunohistochemistry-Paraffin: OCT4 Antibody (OTI9B7) [NBP1-47923] - Staining of paraffin-embedded Human lung tissue using anti-41186 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/OCT4-Antibody-OTI9B7-Immunohistochemistry-Paraffin-NBP1-47923-img0003.jpg)
![Immunohistochemistry: OCT4 Antibody (OTI9B7) [NBP1-47923] - Representative DAB IHC-stained sections of MDBMSCC demonstrating nuclear expression of EMA of cells within the tumor nests [(A), brown]. Expression of SOX2 was seen in cells within the tumor nests [(B), brown, thick arrows] & the stroma [(B), brown, arrowheads], & on the endothelium of the microvessels within the stroma [(B), brown, thin arrows]. Expression of SALL4 was limited to cells within the tumor nests [(C), brown, thick arrows] & the endothelium of the microvessels [(C), brown, thin arrows]. OCT4 was also expressed in cells within tumor nests [(D), brown, thick arrows] & the stroma [(D), brown, arrowheads], & the endothelium of the microvessels within the stroma [(D), brown, thin arrows]. Expression of pSTAT3 was detected on cells within the tumor nests [(E), brown, thick arrows] & the stroma [(E), brown, arrowheads], & the endothelium of the microvessels within the stroma [(E), brown, thin arrows]. NANOG was also seen in cells within the tumor nests [(F), brown, thick arrows] & the stroma [(F), brown, arrowheads], & the endothelium of the microvessels within the stroma [(F), brown, thin arrows]. CD44 expression was seen as membranous staining of the tumor nest cells [(G), brown]. Original magnification: 400×. Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/Article/10.3389/fsurg.2016.00046/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-47923_mouse-oct4-mab-oti9b7-310202416165645.jpg)
![Representative IF IHC-stained sections of MDBMSCC demonstrating the expression of pSTAT3 [(A), red] and EMA [(A), green] by cells within the tumor nests. There was a CSC subpopulation remonstrating nuclear co-expression of STAT3 [(B), red] and CD34 [(B), green], appearing as orange, on the endothelium of the microvessels within the stroma; and another subpopulation staining only positively for pSTAT3 within the stroma [(B), red]. Nuclear expression of NANOG [(C), red] was demonstrated on the endothelium of the microvessels which expressed CD34 [(C), green] within stroma. The NANOG+ cells [(C), red] that do not express CD34 were seen within the tumor nests and the stroma. SOX2 [(D), red] was also expressed by cells within tumor nests and the stroma, and the endothelium of the microvessels expressing CD34 [(D), green]. Nuclear expression of both SOX2 [(E), red] and SALL4 [(E), green], appearing as orange, was seen on the cells within the tumor nests and the stroma. Expression of both SOX2 [(F), red] and OCT4 [(F), green], appearing as orange, was seen on cells within the tumor nests and the stroma, and the endothelium of the microvessels within the stroma. pSTAT3 [(G), red] and membranous staining CD44 [(G), green] were co-expressed by cells within the tumor nests. Scale bars: 20 μm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/27532037), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-47923_mouse-oct4-mab-oti9b7-immunocytochemistry-immunofluorescence-13220261927378.jpg)
![Western Blot: CPT1A Antibody [NB100-53791] - Semiquantitative immunoblotting of CPT1A. Protein expression was investigated in the HB of WT EAE (n=6) and Cpt1a P479L EAE mice (n=6). The data were normalized to the density of the beta actin control. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31527712/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CPT1A-Antibody-Western-Blot-NB100-53791-img0002.jpg "Western Blot: CPT1A Antibody [NB100-53791] - Semiquantitative immunoblotting of CPT1A. Protein expression was investigated in the HB of WT EAE (n=6) and Cpt1a P479L EAE mice (n=6). The data were normalized to the density of the beta actin control. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31527712/) licensed under a CC-BY license.")
![Western Blot: CPT1A Antibody [NB100-53791] - Staining of MCF7 cell lysate (35 ug protein in RIPA buffer). Antibody at 2 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/CPT1A-Antibody-Western-Blot-NB100-53791-img0001.jpg)
![Immunohistochemistry-Paraffin: CPT1A Antibody [NB100-53791] - Negative Control showing staining of paraffin embedded Human Liver, with no primary antibody.](http://images.novusbio.com/fullsize/nb100-53791_goat-polyclonal-cpt1a-antibody-16520239183313.jpg)
![Immunohistochemistry-Paraffin: CPT1A Antibody [NB100-53791] - (5ug/ml) staining of paraffin embedded Human Liver. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/nb100-53791_goat-polyclonal-cpt1a-antibody-16520239183326.jpg)
![Western Blot: Striatin Antibody [NB110-74571] - Immunoblot of PP2A immunoprecipitate with NB110-74571.](http://images.novusbio.com/fullsize/Striatin-Antibody-Western-Blot-NB110-74571-img0004.jpg "Western Blot: Striatin Antibody [NB110-74571] - Immunoblot of PP2A immunoprecipitate with NB110-74571.")
![Immunocytochemistry/Immunofluorescence: Striatin Antibody [NB110-74571] - Immunofluorescent staining of NIH3T3 cells ± blocking peptide using NB110-74571.](http://images.novusbio.com/fullsize/Striatin-Antibody-Immunocytochemistry-Immunofluorescence-NB110-74571-img0003.jpg)
![Western Blot: EGR2 Antibody [NB100-92327] - Analysis of pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:PC12 whole cell lysate(40ug) Lane3:NIH-3T3 whole cell lysate(40ug) Lane3:Jurkat whole cell lysate(40ug)](http://images.novusbio.com/fullsize/EGR2-Antibody-Western-Blot-NB100-92327-img0004.jpg "Western Blot: EGR2 Antibody [NB100-92327] - Analysis of pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:PC12 whole cell lysate(40ug) Lane3:NIH-3T3 whole cell lysate(40ug) Lane3:Jurkat whole cell lysate(40ug)")
![Immunocytochemistry/Immunofluorescence: EGR2 Antibody [NB100-92327] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-EGR2 at 5ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/EGR2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-92327-img0005.jpg)
![Immunohistochemistry-Paraffin: EGR2 Antibody [NB100-92327] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse brain using EGR2 antibody at 1:200 dilution. The signal was detected using HRP-conjugated anti-rabbit secondary antibody and DAB based detection reagent, and the sections were further counterstained with hematoxylin. This EGR2 antibody generated primarily a nuclear staining signal in the neurons and the glial cells, however, some cytoplasmic staining was also observed. The cytoplasmic signal potentially represents the translational or 14-3-3sigma interacting cytoplasmic pool of EGR2.](http://images.novusbio.com/fullsize/EGR2-Antibody-Immunohistochemistry-Paraffin-NB100-92327-img0006.jpg)
![Western Blot: EGR2 Antibody [NB100-92327] - Egr-2 Antibody in extracts from HUVEC cells.](http://images.novusbio.com/fullsize/EGR2-Antibody-Western-Blot-NB100-92327-img0001.jpg)
![Western Blot: HSP27 Antibody [NBP1-75477] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/ml of Rabbit Anti-Human HSP27 Polyclonal Antibody (Catalog # NBP1-75477) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HSP27-Antibody-Knockout-Validated-NBP1-75477-img0010.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP1-75477] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/ml of Rabbit Anti-Human HSP27 Polyclonal Antibody (Catalog # NBP1-75477) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP1-75477] - Analysis of Jurkat cell lysate (30ug) using anti-Hsp27 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Hsp27-Antibody-Western-Blot-NBP1-75477-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody [NBP1-75477] - Hsp27 Antibody [NBP1-75477] - HSP27 antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/Hsp27-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-75477-img0006.jpg)
![Immunohistochemistry: Hsp27 Antibody [NBP1-75477] - Staining of HSP27 in mouse heart using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Hsp27-Antibody-Immunohistochemistry-NBP1-75477-img0007.jpg)
![Western Blot: Hsp27 Antibody [NBP1-75477] - Analysis of HSP27 in human skeletal muscle lysate.](http://images.novusbio.com/fullsize/Hsp27-Antibody-Western-Blot-NBP1-75477-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: MEF2C Antibody [NBP1-89468] - Staining of human cell line RH-30 shows localization to nucleoplasm & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/MEF2C-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89468-img0003.jpg "Immunocytochemistry/Immunofluorescence: MEF2C Antibody [NBP1-89468] - Staining of human cell line RH-30 shows localization to nucleoplasm & vesicles. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: LAMC2 Antibody (CL2980) [NBP2-42388] - Staining in human fallopian tube and liver tissues. Corresponding LAMC2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/LAMC2-Antibody-CL2980-Immunohistochemistry-Paraffin-NBP2-42388-img0023.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: LAMC2 Antibody (CL2980) [NBP2-42388] - Staining in human fallopian tube and liver tissues. Corresponding LAMC2 RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: LAMC2 Antibody (CL2980) [NBP2-42388] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-LAMC2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/LAMC2-Antibody-CL2980-Western-Blot-NBP2-42388-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: LAMC2 Antibody (CL2980) [NBP2-42388] - Staining of A-431 cells using the Anti-LAMC2 monoclonal antibody, showing specific staining in the cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/LAMC2-Antibody-CL2980-Immunocytochemistry-Immunofluorescence-NBP2-42388-img0025.jpg)
![Western Blot: LAMC2 Antibody (CL2980) [NBP2-42388] - Analysis of purified human recombinant Laminin-332, Laminin-421, Laminin-511, Laminin-121 and Laminin-221.](http://images.novusbio.com/fullsize/LAMC2-Antibody-CL2980-Western-Blot-NBP2-42388-img0015.jpg)
![Immunohistochemistry-Paraffin: LAMC2 Antibody (CL2980) [NBP2-42388] - Staining of human skin shows moderate positivity in basement membrane of epidermis.](http://images.novusbio.com/fullsize/LAMC2-Antibody-CL2980-Immunohistochemistry-Paraffin-NBP2-42388-img0018.jpg)
![Immunohistochemistry-Paraffin: LAMC2 Antibody (CL2980) [NBP2-42388] - Staining of human fallopian tube shows moderate positivity in basement membrane of glandular cells.](http://images.novusbio.com/fullsize/LAMC2-Antibody-CL2980-Immunohistochemistry-Paraffin-NBP2-42388-img0019.jpg)
![Immunohistochemistry-Paraffin: LAMC2 Antibody (CL2980) [NBP2-42388] - Staining of human small intestine shows moderate positivity in basement membrane of glandular cells.](http://images.novusbio.com/fullsize/LAMC2-Antibody-CL2980-Immunohistochemistry-Paraffin-NBP2-42388-img0020.jpg)
![Immunohistochemistry-Paraffin: LAMC2 Antibody (CL2980) [NBP2-42388] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/LAMC2-Antibody-CL2980-Immunohistochemistry-Paraffin-NBP2-42388-img0021.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody (J144) [NB100-74564] - Vimentin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Vimentin at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Vimentin-Antibody-J144-Immunocytochemistry-Immunofluorescence-NB100-74564-img0005.jpg "Immunocytochemistry/Immunofluorescence: Vimentin Antibody (J144) [NB100-74564] - Vimentin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Vimentin at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.")
![Immunohistochemistry-Paraffin: Vimentin Antibody (J144) [NB100-74564] - Biopsies of deparaffinized Human melanoma tissue. Cells were probed without (control) or with or an antibody recognizing Vimentin](http://images.novusbio.com/fullsize/Vimentin-Antibody-J144-Immunohistochemistry-Paraffin-NB100-74564-img0003.jpg)
![Western Blot: Vimentin Antibody (J144) [NB100-74564] - Analysis of rat brain cells.](http://images.novusbio.com/fullsize/Vimentin-Antibody-J144-Western-Blot-NB100-74564-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody (J144) [NB100-74564] - Vimentin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Vimentin at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Vimentin-Antibody-J144-Immunocytochemistry-Immunofluorescence-NB100-74564-img0007.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (J144) [NB100-74564] - Both normal and cancer biopsies of deparaffinized Human tonsil tissue.](http://images.novusbio.com/fullsize/Vimentin-Antibody-J144-Immunohistochemistry-Paraffin-NB100-74564-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody (J144) [NB100-74564] - Vimentin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Vimentin at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Vimentin-Antibody-J144-Immunocytochemistry-Immunofluorescence-NB100-74564-img0006.jpg)
![Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP2-15336] - PG-4 whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A1 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-1-A1-ALDH1A1-Antibody-Western-Blot-NBP2-15336-img0021.jpg "Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP2-15336] - PG-4 whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A1 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP2-15336] - Analysis of methanol-fixed A549, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/ALDH1A1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15336-img0001.jpg)
![Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP2-15336] - Detects ALDH1A1 protein by Western blot analysis. A. 50 ug rat liver lysate/extract 10 % SDS-PAGE Dilution: 1:5000](http://images.novusbio.com/fullsize/ALDH1A1-Antibody-Western-Blot-NBP2-15336-img0012.jpg)
![Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP2-15336] - Detects ALDH1A1 protein by Western blot analysis. A. 50 ug mouse liver lysate/extract 10 % SDS-PAGE Dilution: 1:5000](http://images.novusbio.com/fullsize/ALDH1A1-Antibody-Western-Blot-NBP2-15336-img0015.jpg)
![Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP2-15336] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A1 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-1-A1-ALDH1A1-Antibody-Western-Blot-NBP2-15336-img0018.jpg)
![Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP2-15336] - Human gastric cancer, using ALDH1A1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/ALDH1A1-Antibody-Immunohistochemistry-Paraffin-NBP2-15336-img0002.jpg)
![Immunohistochemistry-Paraffin: ATG12 Antibody [NBP2-15501] - Human lung cancer. ATG12 stained by ATG12 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/ATG12-Antibody-Immunohistochemistry-Paraffin-NBP2-15501-img0015.jpg "Immunohistochemistry-Paraffin: ATG12 Antibody [NBP2-15501] - Human lung cancer. ATG12 stained by ATG12 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: ATG12 Antibody [NBP2-15501] - Sample (30 ug of whole cell lysate) A: NIH-3T3 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/ATG12-Antibody-Western-Blot-NBP2-15501-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ATG12 Antibody [NBP2-15501] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ATG12 antibody.](http://images.novusbio.com/fullsize/ATG12-Antibody-Western-Blot-NBP2-15501-img0011.jpg)
![Western Blot: ATG12 Antibody [NBP2-15501] - Sample (50 ug of whole cell lysate) A: Rat brain 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ATG12-Antibody-Western-Blot-NBP2-15501-img0014.jpg)
![Immunohistochemistry-Paraffin: ATG12 Antibody [NBP2-15501] - Rat brain. ATG12 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/ATG12-Antibody-Immunohistochemistry-Paraffin-NBP2-15501-img0008.jpg)
![Immunoprecipitation: ATG12 Antibody [NBP2-15501] - Immunoprecipitation of Atg 12-Atg5 protein from 293T whole cell extracts using 5 ug of ATG12 antibody. Western blot analysis was performed using ATG12 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/ATG12-Antibody-Immunoprecipitation-NBP2-15501-img0006.jpg)
![Western Blot: ATG12 Antibody [NBP2-15501] -Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with ATG12 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp2-15501_rabbit-polyclonal-atg12-antibody-western-blot-81202481244..jpg)
![Western Blot: Brg1 Antibody [NB100-2594] - Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRG1/SMARCA4 antibodyused for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30seconds.](http://images.novusbio.com/fullsize/Brg1-Antibody-Western-Blot-NB100-2594-img0019.jpg "Western Blot: Brg1 Antibody [NB100-2594] - Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRG1/SMARCA4 antibodyused for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30seconds.")
![Immunohistochemistry-Paraffin: Brg1 Antibody [NB100-2594] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit antiBRG1/SMARCA4 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Brg1-Antibody-Immunohistochemistry-Paraffin-NB100-2594-img0017.jpg)
![Western Blot: Brg1 Antibody [NB100-2594] - Whole cell lysate (15 ug) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRG1/SMARCA4 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Brg1-Antibody-Western-Blot-NB100-2594-img0018.jpg)
![Immunohistochemistry-Paraffin: Brg1 Antibody [NB100-2594] - Section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit antiBRG1/SMARCA4 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Brg1-Antibody-Immunohistochemistry-Paraffin-NB100-2594-img0016.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Brg1 Antibody [NB100-2594] - Detection of human BRG1-SMARCA4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BRG1-SMARCA4 antibody NB100-2594 used for IP at 3 ug per reaction. BRG1-SMARCA4 was also immunoprecipitated by rabbit anti-BRG1-SMARCA4 antibody NBP-22234. For blotting immunoprecipitated BRG1-SMARCA4, NB100-2594 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Brg1-Antibody-Immunoprecipitation-NB100-2594-img0020.jpg)