EGR2 Antibody

Western Blot: EGR2 Antibody [NB100-92327] - Analysis of pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:PC12 whole cell lysate(40ug) Lane3:NIH-3T3 whole cell lysate(40ug) Lane3:Jurkat whole cell lysate(40ug)

Immunocytochemistry/Immunofluorescence: EGR2 Antibody [NB100-92327] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-EGR2 at 5ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry-Paraffin: EGR2 Antibody [NB100-92327] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse brain using EGR2 antibody at 1:200 dilution. The signal was detected using HRP-conjugated anti-rabbit secondary antibody and DAB based detection reagent, and the sections were further counterstained with hematoxylin. This EGR2 antibody generated primarily a nuclear staining signal in the neurons and the glial cells, however, some cytoplasmic staining was also observed. The cytoplasmic signal potentially represents the translational or 14-3-3sigma interacting cytoplasmic pool of EGR2.

Western Blot: EGR2 Antibody [NB100-92327] - Egr-2 Antibody in extracts from HUVEC cells.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

EGR2 Antibody Summary

• Immunogen: Synthetic peptide made to an internal portion of the human EGR2 protein (between amino acids 370-420) [UniProt P11161].
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EGR2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 - 2 ug/ml
  • Immunocytochemistry/Immunofluorescence 5 - 10 ug/ml
  • Immunohistochemistry 2 - 5 ug/ml
  • Immunohistochemistry-Paraffin 2 - 5 ug/ml
• Application Notes: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for EGR2 Antibody

• AT591
• CMT1D
• CMT4E
• early growth response 2 (Krox-20 homolog, Drosophila)
• early growth response 2
• early growth response protein 2
• EGR-2
• FLJ14547
• Krox-20 homolog, Drosophila
• KROX-20, Drosophila, homolog (early growth response-2)
• KROX20DKFZp686J1957
• Zinc finger protein Krox-20

Background

Egr proteins function in transcription regulatory activities surrounding cellular growth, differentiation and function. The deduced amino acid sequences of human Egr-2 and mouse Egr-1 are 92% identical in the zinc finger region but show no homology elsewhere. Egr-2 is a sequence-specific DNA-binding transcription factor that binds two specific DNA sites located in the promoter region of HoxA4 and localizes to the nucleus. Defects in the Egr-2 protein are a cause of congenital hypomyelination neuropathy (CHN). CHN is characterized clinically by early onset of hypotonia, areflexia, distal muscle weakness and very slow nerve conduction velocities. Mutations in the gene that encodes Egr-2 (EGR2) also cause of Dejerine-Sottas syndrome (DSS), which is also known as Dejerine-Sottas neuropathy (DSN) or hereditary motor and sensory neuropathy III (HMSN3). DSS patients exhibit severe early onset motor and sensory neuropathy with very slow nerve conduction velocities and elevated cerebrospinal fluid protein concentrations.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for EGR2 Antibody (NB100-92327) (1)

Average Rating: 1

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Susumu Tasaki IHC-Fr Mouse 12/05/2019

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Frozen mouse sciatic nerves,Frozen mouse sciatic nerve
• Species: Mouse
• Lot: A-2

Comments

• Comments: Novus Innovators Program - new species or application used on a primary antibody.

FAQs for EGR2 Antibody (NB100-92327). (Showing 1 - 1 of 1 FAQ).

1. We would like to try this antibody for staining in Xenopus. The mentioned immunogenic region shows high sequence identity with Xenopus EGR2. Would it be possible to obtain the exact epitope to predict cross reactivity?
   • I have now been informed that the immunogen sequence for this antibody corresponds to the amino acid range 391-440 of P11161. This shows high homology to the Xenopus sequence, so it is likely that the antibody would bind to Xenopus protein.

Bioinformatics

• Gene Symbol: EGR2
• Entrez:
  • 1959(Human)
  • 114090(Rat)
• Uniprot:
  • P11161(Human)