| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-139SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77679SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56411SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-55244SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-111SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77687SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54792SS | |||
| Azide and BSA Free | NBP2-80556 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70169 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-60735SS | |||
| Azide and BSA Free | NBP2-80831 | |||
![Western Blot: TDO2 Antibody [H00006999-B01P] - Specificity of antibodies for tryptophan 2,3-dioxygenase (TDO) by Western blot. The total protein (20 ug) of the dentate gyrus, extracted from the wild-type (lanes 1 and 3) and Tdo-/- mice (lane 2), was subjected to 4% to 12% gradient sodium dodecyl sulfate polyacrylamide gel electrophoresis, and transferred to polyvinylidene difluoride membranes. TDO was detected as described in the Methods section. Note that a 45-kDa band was detected with the anti-TDO antibody. The positions of molecular weight markers are shown on the left. Lane 3: negative control without the primary antibody. beta-Tubulin is a positive control for the Western blot. Image collected and cropped by CiteAb from the following publication (//molecularbrain.biomedcentral.com/articles/10.1186/1756-6606-3-26), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TDO2-Antibody-Western-Blot-H00006999-B01P-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: TDO2 Antibody [H00006999-B01P] - Specificity of antibodies for tryptophan 2,3-dioxygenase (TDO) by Western blot. The total protein (20 ug) of the dentate gyrus, extracted from the wild-type (lanes 1 and 3) and Tdo-/- mice (lane 2), was subjected to 4% to 12% gradient sodium dodecyl sulfate polyacrylamide gel electrophoresis, and transferred to polyvinylidene difluoride membranes. TDO was detected as described in the Methods section. Note that a 45-kDa band was detected with the anti-TDO antibody. The positions of molecular weight markers are shown on the left. Lane 3: negative control without the primary antibody. beta-Tubulin is a positive control for the Western blot. Image collected and cropped by CiteAb from the following publication (//molecularbrain.biomedcentral.com/articles/10.1186/1756-6606-3-26), licensed under a CC-BY license.")
![<b>Genetic Strategies Validation. </b>Western Blot: TDO2 Antibody [H00006999-B01P] - Analysis of TDO2 expression in transfected 293T cell line by TDO2 polyclonal antibody. Lane 1: TDO2 transfected lysate(44.66 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/TDO2-Antibody-Western-Blot-H00006999-B01P-img0005.jpg)
![Immunohistochemistry: TDO2 Antibody [H00006999-B01P] - Immunoreactivity of TDO in the SVZ, the olfactory bulb, and the cerebellum. In the cerebellum, the immunoreactivity for TDO was observed in granule cells and Purkinje cells of wild type mice, but these positive structures disappeared in Tdo-/- mice. The cytoarchitectural organization of Tdo-/- mice was the same as that of wild type mice. Image collected and cropped by CiteAb from the following publication (//molecularbrain.biomedcentral.com/articles/10.1186/1756-6606-3-26), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TDO2-Antibody-Immunocytochemistry-Immunofluorescence-H00006999-B01P-img0009.jpg)
![Western Blot: TDO2 Antibody [H00006999-B01P] - Analysis of TDO2 expression in human liver.](http://images.novusbio.com/fullsize/TDO2-Antibody-Western-Blot-H00006999-B01P-img0004.jpg)
![Immunohistochemistry: TDO2 Antibody [H00006999-B01P] - Immunoreactivity of TDO in the SVZ, the olfactory bulb, and the cerebellum. A TDO was not stained in the SVZ. The immunoreactivities for TDO in wild type and Tdo-/- mice were background level. Image collected and cropped by CiteAb from the following publication (//molecularbrain.biomedcentral.com/articles/10.1186/1756-6606-3-26), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TDO2-Antibody-Immunocytochemistry-Immunofluorescence-H00006999-B01P-img0007.jpg)
![Immunohistochemistry: TDO2 Antibody [H00006999-B01P] - Immunoreactivity of TDO in the SVZ, the olfactory bulb, and the cerebellum. TDO was not stained in the olfactory bulb (OB). The immunoreactivities for TDO in wild type and Tdo-/- mice were background level. Image collected and cropped by CiteAb from the following publication (//molecularbrain.biomedcentral.com/articles/10.1186/1756-6606-3-26), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TDO2-Antibody-Immunocytochemistry-Immunofluorescence-H00006999-B01P-img0008.jpg)
![Immunohistochemistry: TDO2 Antibody [H00006999-B01P] - Specificity of antibodies for tryptophan 2,3-dioxygenase (TDO) by immunofluorescent staining. The hippocampal sections of wild-type and Tdo-/- mice were stained with anti-TDO antibody. In sections of Tdo -/- mice, immunofluorescence signals were not detected in granule cells, interneurons (arrowhead), CA1, or CA3 cells, suggesting that the secondary antibody used in this study bound specifically to the primary antibody (mouse IgG). g, granule cell layer; h, hilus. Image collected and cropped by CiteAb from the following publication (//molecularbrain.biomedcentral.com/articles/10.1186/1756-6606-3-26), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TDO2-Antibody-Immunohistochemistry-H00006999-B01P-img0010.jpg)
![Immunocytochemistry/ Immunofluorescence: TDO2 Antibody [H00006999-B01P] - Immunoreactivity of TDO in the SVZ, the olfactory bulb, & the cerebellum. A & B: TDO was not stained in the SVZ (A) & the olfactory bulb (OB) (B). The immunoreactivities for TDO in wild type & Tdo-/- mice were background level. C: In the cerebellum, the immunoreactivity for TDO was observed in granule cells & Purkinje cells of wild type mice, but these positive structures disappeared in Tdo-/- mice. The cytoarchitectural organization of Tdo-/- mice was the same as that of wild type mice. E, external plexiform layer; G, granule cell layer; Gm, glomerular layer; I, internal plexiform layer; LV, lateral ventricle; Mi, mitral cell layer; Mo, molecular layer; P, Purkinje cell layer; St, striatum; Su, subependymal zone. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/20815922), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00006999-b01p_mouse-polyclonal-tdo2-antibody-310202415384127.jpg)
![Immunocytochemistry/ Immunofluorescence: TDO2 Antibody [H00006999-B01P] - Immunoreactivity of TDO in the SVZ, the olfactory bulb, & the cerebellum. A & B: TDO was not stained in the SVZ (A) & the olfactory bulb (OB) (B). The immunoreactivities for TDO in wild type & Tdo-/- mice were background level. C: In the cerebellum, the immunoreactivity for TDO was observed in granule cells & Purkinje cells of wild type mice, but these positive structures disappeared in Tdo-/- mice. The cytoarchitectural organization of Tdo-/- mice was the same as that of wild type mice. E, external plexiform layer; G, granule cell layer; Gm, glomerular layer; I, internal plexiform layer; LV, lateral ventricle; Mi, mitral cell layer; Mo, molecular layer; P, Purkinje cell layer; St, striatum; Su, subependymal zone. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/20815922), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00006999-b01p_mouse-polyclonal-tdo2-antibody-310202415171317.jpg)
![Immunocytochemistry/ Immunofluorescence: TDO2 Antibody [H00006999-B01P] - Immunoreactivity of TDO in the SVZ, the olfactory bulb, & the cerebellum. A & B: TDO was not stained in the SVZ (A) & the olfactory bulb (OB) (B). The immunoreactivities for TDO in wild type & Tdo-/- mice were background level. C: In the cerebellum, the immunoreactivity for TDO was observed in granule cells & Purkinje cells of wild type mice, but these positive structures disappeared in Tdo-/- mice. The cytoarchitectural organization of Tdo-/- mice was the same as that of wild type mice. E, external plexiform layer; G, granule cell layer; Gm, glomerular layer; I, internal plexiform layer; LV, lateral ventricle; Mi, mitral cell layer; Mo, molecular layer; P, Purkinje cell layer; St, striatum; Su, subependymal zone. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/20815922), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00006999-b01p_mouse-polyclonal-tdo2-antibody-310202415394527.jpg)
![Western Blot: TDO2 Antibody [H00006999-B01P] - Specificity of antibodies for tryptophan 2,3-dioxygenase (TDO) by Western blot (A) & immunofluorescent staining (B). A: The total protein (20 μg) of the dentate gyrus, extracted from the wild-type (lanes 1 & 3) & Tdo-/- mice (lane 2), was subjected to 4% to 12% gradient sodium dodecyl sulfate polyacrylamide gel electrophoresis, & transferred to polyvinylidene difluoride membranes. TDO was detected as described in the Methods section. Note that a 45-kDa band was detected with the anti-TDO antibody. The positions of molecular weight markers are shown on the left. Lane 3: negative control without the primary antibody. beta -Tubulin is a positive control for the Western blot. B: The hippocampal sections of wild-type & Tdo-/- mice were stained with anti-TDO antibody. In sections of Tdo-/- mice, immunofluorescence signals were not detected in granule cells, interneurons (arrowhead), CA1, or CA3 cells, suggesting that the secondary antibody used in this study bound specifically to the primary antibody (mouse IgG). g, granule cell layer; h, hilus. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/20815922), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00006999-b01p_mouse-polyclonal-tdo2-antibody-310202415483615.jpg)
![Immunohistochemistry: TDO2 Antibody [H00006999-B01P] - Specificity of antibodies for tryptophan 2,3-dioxygenase (TDO) by Western blot (A) & immunofluorescent staining (B). A: The total protein (20 μg) of the dentate gyrus, extracted from the wild-type (lanes 1 & 3) & Tdo-/- mice (lane 2), was subjected to 4% to 12% gradient sodium dodecyl sulfate polyacrylamide gel electrophoresis, & transferred to polyvinylidene difluoride membranes. TDO was detected as described in the Methods section. Note that a 45-kDa band was detected with the anti-TDO antibody. The positions of molecular weight markers are shown on the left. Lane 3: negative control without the primary antibody. beta -Tubulin is a positive control for the Western blot. B: The hippocampal sections of wild-type & Tdo-/- mice were stained with anti-TDO antibody. In sections of Tdo-/- mice, immunofluorescence signals were not detected in granule cells, interneurons (arrowhead), CA1, or CA3 cells, suggesting that the secondary antibody used in this study bound specifically to the primary antibody (mouse IgG). g, granule cell layer; h, hilus. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/20815922), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00006999-b01p_mouse-polyclonal-tdo2-antibody-310202415485113.jpg)
![Western Blot: Apolipoprotein A5 Antibody (1G5G9) [NB400-139] - Analysis of ApoA5 in HepG2 lysates.](http://images.novusbio.com/fullsize/Apolipoprotein-A5-Antibody-1G5G9-Western-Blot-NB400-139-img0005.jpg "Western Blot: Apolipoprotein A5 Antibody (1G5G9) [NB400-139] - Analysis of ApoA5 in HepG2 lysates.")
![Immunocytochemistry/Immunofluorescence: Apolipoprotein A5 Antibody (1G5G9) [NB400-139] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Apolipoprotein A5 (1G5G9) at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Apolipoprotein-A5-Antibody-1G5G9-Immunocytochemistry-Immunofluorescence-NB400-139-img0006.jpg)
![Flow Cytometry: Apolipoprotein A5 Antibody (1G5G9) [NB400-139] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Apolipoprotein A5 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Apolipoprotein-A5-Antibody-1G5G9-Flow-Cytometry-NB400-139-img0004.jpg)
![Western Blot: Apolipoprotein A5 Antibody (1G5G9) [NB400-139] - Analysis using anti- human APOA5 monoclonal antibody against human serum total protein (lane 2). Lane 1 is a MW standard.](http://images.novusbio.com/fullsize/Apolipoprotein-A5-Antibody-1G5G9-Western-Blot-NB400-139-img0003.jpg)
![Western Blot: Apolipoprotein A5 Antibody (1G5G9) [NB400-139] - Detection of Apo A5 in HepG2 cell lysate with NB400-139.](http://images.novusbio.com/fullsize/Apolipoprotein-A5-Antibody-1G5G9-Western-Blot-NB400-139-img0002.jpg)
![Western Blot: IGF-I R Antibody [NBP1-77679] - Analysis of IGF-1R in 1. HeLa, 2. Ntera2, 3. A431, 4. HepG2, 5. MCF7, 6. NIH/3T3, 7. SK-BR-3 and 8. COS7](http://images.novusbio.com/fullsize/IGF-I-R-Antibody-Western-Blot-NBP1-77679-img0009.jpg "Western Blot: IGF-I R Antibody [NBP1-77679] - Analysis of IGF-1R in 1. HeLa, 2. Ntera2, 3. A431, 4. HepG2, 5. MCF7, 6. NIH/3T3, 7. SK-BR-3 and 8. COS7")
![Immunocytochemistry/Immunofluorescence: IGF-I R Antibody [NBP1-77679] - IGF-1 R antibody was tested in A431 cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/IGF-I-R-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77679-img0008.jpg)
![Immunohistochemistry-Paraffin: IGF-I R Antibody [NBP1-77679] - IHC analysis of a formalin fixed paraffin embedded tissue section of the human kidney cancer xenograft using 1:200 dilution of IFG-I R antibody (NBP1-77679). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin.](http://images.novusbio.com/fullsize/IGF-I-R-Antibody-Immunohistochemistry-Paraffin-NBP1-77679-img0010.jpg)
![Simple Western: IGF-I R Antibody [NBP1-77679] - Simple Western lane view shows a specific band for IGF-1 R in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/IGF-I-R-Antibody-Simple-Western-NBP1-77679-img0007.jpg)
![Western Blot: GRP78/HSPA5 Antibody [NB100-56411] - Analysis of GRP78/HSPA5 in A) HeLa, B) human liver, C) mouse liver, and D) rat liver lysate using NB100-56411 at 2 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were use for this test.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NB100-56411-img0004.jpg "Western Blot: GRP78/HSPA5 Antibody [NB100-56411] - Analysis of GRP78/HSPA5 in A) HeLa, B) human liver, C) mouse liver, and D) rat liver lysate using NB100-56411 at 2 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were use for this test.")
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NB100-56411] - Analysis of GRP78/HSPA5 in formalin-fixed, paraffin-embedded mouse liver tissue using this antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NB100-56411-img0002.jpg)
![Western Blot: GRP78/HSPA5 Antibody [NB100-56411] - Analysis of GRP78/HSPA5 in mouse liver tissue enriched using Endoplasmic Reticulum Enrichment Kit and 1 ug/ml of NB100-56411 and 0.25 ug/ml of anti-GAPDH loading control. Lane A contains 20 ugs of whole mouse liver lysate, lane B contains 20 ugs of total ER fraction, and lane C contains 20 ugs of rough ER fraction.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NB100-56411-img0003.jpg)
![Simple Western: GRP78/HSPA5 Antibody [NB100-56411] - Simple Western lane view shows a specific band for GRP78/HSPA5 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Simple-Western-NB100-56411-img0005.jpg)
![Western Blot: INSIG-1 Antibody [NB110-55244] - Detection of Insig 1 in HepG2 lyaste using NB110-55244.](http://images.novusbio.com/fullsize/INSIG-1-Antibody-Western-Blot-NB110-55244-img0002.jpg "Western Blot: INSIG-1 Antibody [NB110-55244] - Detection of Insig 1 in HepG2 lyaste using NB110-55244.")
![Immunocytochemistry/Immunofluorescence: INSIG-1 Antibody [NB110-55244] - INSIG-1 antibody was tested in HepG2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/INSIG-1-Antibody-Immunocytochemistry-Immunofluorescence-NB110-55244-img0003.jpg)
![Western Blot: Endothelial Lipase Antibody [NB400-111] - Human endothelial lipase detected in transfected 293 lysates (MW 57 kDa)](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-Western-Blot-NB400-111-img0007.jpg "Western Blot: Endothelial Lipase Antibody [NB400-111] - Human endothelial lipase detected in transfected 293 lysates (MW 57 kDa)")
![Immunocytochemistry/Immunofluorescence: Endothelial Lipase Antibody [NB400-111] - Antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-Immunocytochemistry-Immunofluorescence-NB400-111-img0001.jpg)
![Western Blot: Endothelial Lipase Antibody [NB400-111] - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY LIPG cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIPG.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-Western-Blot-NB400-111-img0006.jpg)
![Western Blot: IRE1 alpha Antibody Pack [NBP2-50067] - Western Blot: IRE1 alpha [p Ser724] Antibody [NB100-2323] - Western blot of anti-IRE1 alpha (pSer724) using Lot AN and AO of NB100-2323. HeLa cells were treated (+) or untreated (-) with 10 mM DTT for 60 min to activate the UPR. Total protein was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% BSA in TBST. The membrane was probed with 2.0 ug/ml antibody in 5% BSA, and detected with an anti-rabbit HRP secondary antibody using chemiluminecence. Theoretical molecular weight: 110 kDa.](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Pack-Western-Blot-NBP2-50067-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: IRE1 alpha Antibody Pack [NBP2-50067] - Western Blot: IRE1 alpha [p Ser724] Antibody [NB100-2323] - Western blot of anti-IRE1 alpha (pSer724) using Lot AN and AO of NB100-2323. HeLa cells were treated (+) or untreated (-) with 10 mM DTT for 60 min to activate the UPR. Total protein was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% BSA in TBST. The membrane was probed with 2.0 ug/ml antibody in 5% BSA, and detected with an anti-rabbit HRP secondary antibody using chemiluminecence. Theoretical molecular weight: 110 kDa.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: IRE1 alpha Antibody Pack [NBP2-50067] - P-Ire1a activation correlates with WFS1 cell expression. Cryosections of retina from 12 month old Wfs1+/+ and Wfs1-/- mouse were immunostained with anti-P-Ire1a antibody (red). DAPI was used for staining of cell nuclei (blue). RPE, retinal pigment epithelium; ONL, outer nuclear layer; INL, inner nuclear layer, RGC, retinal ganglion cells. Scale bars=50 um. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0097222) licensed under a CC-BY license. IRE1 alpha [p Ser724] Antibody [NB100-2323]](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Pack-Immunohistochemistry-NBP2-50067-img0006.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: IRE1 alpha Antibody Pack [NBP2-50067] - Western Blot detection of Total IRE1 Alpha in transfected Min6 cells using IRE1 alpha Antibody [NB100-2324]](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Pack-Western-Blot-NBP2-50067-img0002.jpg)
![Immunohistochemistry: IRE1 alpha Antibody Pack [NBP2-50067] - XBP1 and TG2 deficiency promote increased IRE1a protein levels in cultured VSMCs and in neointima in situ. (D) Xbp1WT and Xbp1 delta WT and (E) Tgm2 WT and Tgm2 delta carotid artery sections were analyzed by IHC for total IRE1a. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0212235) licensed under a CC-BY license. IRE1 alpha Antibody [NB100-2324]](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Pack-Immunohistochemistry-NBP2-50067-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: ABCB5 Antibody [NBP1-77687] - ABCB5 antibody was tested in A431 cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/ABCB5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77687-img0004.jpg "Immunocytochemistry/Immunofluorescence: ABCB5 Antibody [NBP1-77687] - ABCB5 antibody was tested in A431 cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).")
![Immunohistochemistry: ABCB5 Antibody [NBP1-77687] - Analysis of ABCB5 in mouse spleen using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/ABCB5-Antibody-Immunohistochemistry-NBP1-77687-img0003.jpg)
![Western Blot: ABCA1 Antibody (1276B) [NBP2-54792] - Mouse Raw264.7 and human THP-1 cells were treated with and without the LXR agonist TO901317 (1 uM) for 24 hours. Total protein was then separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/ml anti-ABCA1 in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/ABCA1-Antibody-1276B-Western-Blot-NBP2-54792-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: ABCA1 Antibody (1276B) [NBP2-54792] - Mouse Raw264.7 and human THP-1 cells were treated with and without the LXR agonist TO901317 (1 uM) for 24 hours. Total protein was then separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/ml anti-ABCA1 in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: ABCA1 Antibody (1276B) [NBP2-54792] - Analysis of a FFPE tissue section of mouse liver using ABCA1 antibody (1276B) Lot ckjv02 at 1:100 dilution. The primary antibody bound to ABCA1 antigen in the tissue section was detected using a HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. This ABCA1 antibody generated specific staining in the membranes of the hepatocytes and the Kupffer cells. As expected for ABCA1, the staining signal was stronger in the Kupffer cells which are the tissue macrophages in liver.](http://images.novusbio.com/fullsize/ABCA1-Antibody-1276B-Immunohistochemistry-Paraffin-NBP2-54792-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: ABCA1 Antibody (1276B) [NBP2-54792] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-ABCA1 at 10 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/ABCA1-Antibody-1276B-Immunocytochemistry-Immunofluorescence-NBP2-54792-img0003.jpg)
![Flow (Intracellular): ABCA1 Antibody (1276B) [NBP2-54792] - An intracellular stain was performed on HepG2 Cells with ABCA1 (1276B) antibody NBP2-54792 (blue) and a matched isotype control MAB1050 (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by rabbit IgG APC-conjugated secondary antibody (F0111, R&D Systems).](http://images.novusbio.com/fullsize/ABCA1-Antibody-1276B-Flow-Intracellular-NBP2-54792-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: ABCA1 Antibody (1276B) [NBP2-54792] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-ABCA1 at 10 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/ABCA1-Antibody-1276B-Immunocytochemistry-Immunofluorescence-NBP2-54792-img0002.jpg)
![Immunohistochemistry-Paraffin: ABCA1 Antibody (1276B) [NBP2-54792] - Analysis of a FFPE tissue section of mouse liver using ABCA1 antibody (1276B) Lot ckjv0116091 at 1:50 dilution. The primary antibody bound to ABCA1 antigen in the tissue section was detected using a HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. This ABCA1 antibody generated specific staining in the membranes of the hepatocytes and the Kupffer cells. As expected for ABCA1, the staining signal was stronger in the Kupffer cells which are the tissue macrophages in liver.](http://images.novusbio.com/fullsize/ABCA1-Antibody-1276B-Immunohistochemistry-Paraffin-NBP2-54792-img0005.jpg)
![Western Blot: 15-Lipoxygenase 1 Antibody (OTI3G8) [NBP2-01740] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-ALOX15 monoclonal antibody.(1:200)](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-OTI3G8-Western-Blot-NBP2-01740-img0012.jpg "Western Blot: 15-Lipoxygenase 1 Antibody (OTI3G8) [NBP2-01740] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-ALOX15 monoclonal antibody.(1:200)")
![Immunocytochemistry/Immunofluorescence: 15-Lipoxygenase 1 Antibody (OTI3G8) [NBP2-01740] - COS7 cells](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-3G8-Immunocytochemistry-Immunofluorescence-NBP2-01740-img0011.jpg)
![Immunohistochemistry-Paraffin: 15-Lipoxygenase 1 Antibody (OTI3G8) [NBP2-01740] - Staining of paraffin-embedded Human endometrium tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-3G8-Immunohistochemistry-Paraffin-NBP2-01740-img0008.jpg)
![Western Blot: 15-Lipoxygenase 1 Antibody (OTI3G8) [NBP2-01740] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY 15 Lipoxygenase 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-15 Lipoxygenase 1.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-3G8-Western-Blot-NBP2-01740-img0001.jpg)
![Western Blot: 15-Lipoxygenase 1 Antibody (OTI3G8) [NBP2-01740] - Analysis of extracts (35ug) from 9 different cell lines by using anti-15 Lipoxygenase 1 monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-3G8-Western-Blot-NBP2-01740-img0009.jpg)
![Immunohistochemistry-Paraffin: 15-Lipoxygenase 1 Antibody (OTI3G8) [NBP2-01740] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-3G8-Immunohistochemistry-Paraffin-NBP2-01740-img0002.jpg)
![Immunohistochemistry-Paraffin: 15-Lipoxygenase 1 Antibody (OTI3G8) [NBP2-01740] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-3G8-Immunohistochemistry-Paraffin-NBP2-01740-img0003.jpg)
![Immunohistochemistry-Paraffin: 15-Lipoxygenase 1 Antibody (OTI3G8) [NBP2-01740] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-3G8-Immunohistochemistry-Paraffin-NBP2-01740-img0004.jpg)
![Immunohistochemistry-Paraffin: 15-Lipoxygenase 1 Antibody (OTI3G8) [NBP2-01740] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-3G8-Immunohistochemistry-Paraffin-NBP2-01740-img0005.jpg)
![Immunohistochemistry-Paraffin: 15-Lipoxygenase 1 Antibody (OTI3G8) [NBP2-01740] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-3G8-Immunohistochemistry-Paraffin-NBP2-01740-img0006.jpg)
![Immunohistochemistry-Paraffin: 15-Lipoxygenase 1 Antibody (OTI3G8) [NBP2-01740] - Staining of paraffin-embedded Human breast tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-3G8-Immunohistochemistry-Paraffin-NBP2-01740-img0007.jpg)
![Western Blot: LC3B Antibody (1251B) [NBP2-60735] - Western blot analysis of HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50uM Chloroquine for 18 hours. PVDF (polyvinylidene difluoride) membrane was probed with 0.1 ug/mL of anti-LC3B monoclonal antibody (Catalog # NBP2-60735) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for LC3B at approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Knockout-Validated-NBP2-60735-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: LC3B Antibody (1251B) [NBP2-60735] - Western blot analysis of HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50uM Chloroquine for 18 hours. PVDF (polyvinylidene difluoride) membrane was probed with 0.1 ug/mL of anti-LC3B monoclonal antibody (Catalog # NBP2-60735) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for LC3B at approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Simple Western: LC3B Antibody (1251B) [NBP2-60735] - Lane view shows lysates of NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 50uM Chloroquine for 18 hours, loaded at 0.2 mg/mL. A specific band was detected for LC3B at approximately 17 kDa (as indicated) using 5 ug/mL of Rabbit Anti-Human/Mouse/Rat anti-LC3B Monoclonal Antibody (1251B) [Catalog # NBP2-60735]. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Simple-Western-NBP2-60735-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: LC3B Antibody (1251B) [NBP2-60735] - HeLa cells were treated with 50uM CQ (Chloroquine) overnight, fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti- [Catalog # NBP2-60735] at 2 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Immunocytochemistry-Immunofluorescence-NBP2-60735-img0007.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: LC3B Antibody (1251B) [NBP2-60735] - Total protein from HeLa and Neuro2A cells treated with or without 50 uM chloroquine (CQ) for 24 hours was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF (Polyvinylidene difluoride) membrane and blocked in 5% non-fat milk in TBST (Tris-buffered saline, 0.1% Tween 20). The membrane was probed with 12.0 ug/ml anti-LC3B Antibody (1251B) [Catalog # NBP2-60735] in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Western-Blot-NBP2-60735-img0010.jpg)
![Immunohistochemistry-Paraffin: LC3B Antibody (1251B) [NBP2-60735] - Analysis of a FFPE (Formalin-Fixed Paraffin-Embedded) tissue section of mouse brain using anti-LC3B antibody (clone 1251B) [Catalog # NBP2-60735] at 5ug/ml concentration (1:200 dilution). The primary antibody binding to LC3 in cells was detected using HRP conjugated anti-Rabbit secondary antibody with DAB (3,3'Diaminobenzidine) reagent, and the sections were further counterstained with hematoxylin for labeling cellular nuclei. This LC3 antibody generated a diffused staining in all cell types (except the endothelial cells of blood vessels) and the signal was strongest in a subset of neuronal cells. A few cells depicted LC3 puncta formation/dotted staining in apparently autophagic cells.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Immunohistochemistry-Paraffin-NBP2-60735-img0011.jpg)
![<b>Biological Strategies Validation. </b>Flow (Intracellular): LC3B Antibody (1251B) [NBP2-60735] - HeLa cells were either (A) untreated or (B) treated with 50uM chloroquine for 24 hours. An intracellular stain was performed with anti-LC3B (1251B) antibody [Catalog # NBP2-60735] (blue) and a matched isotype control [Catalog # MAB1050] (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by rabbit IgG APC-conjugated secondary antibody (F0111, R&D Systems).](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Flow-Intracellular-NBP2-60735-img0009.jpg)
![Western Blot: GAPDH Antibody [NB300-325] - Detection of mouse and rat GAPDH by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-GAPDH antibody NB300-325 used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-325-img0006.jpg "Western Blot: GAPDH Antibody [NB300-325] - Detection of mouse and rat GAPDH by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-GAPDH antibody NB300-325 used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunocytochemistry/Immunofluorescence: GAPDH Antibody [NB300-325] - GAPDH antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/GAPDH-Antibody-Immunocytochemistry-Immunofluorescence-NB300-325-img0003.jpg)
![Western Blot: GAPDH Antibody [NB300-325] - Whole cell lysate (50 ug) from HeLa (H) and 293T (50 ug) probed with GAPDH Antibody diluted at 0.04 ug/ml](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-325-img0001.jpg)
![Western Blot: GAPDH Antibody [NB300-325] - Detection of Mouse GAPDH Whole cell lysate (5, 15 and 50 ug) from mouse NIH3T3 cells. Another Affinity purified rabbit anti-GAPDH used at 0.04 mcg/ml. Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-325-img0005.jpg)
![Simple Western: GAPDH Antibody [NB300-325] - Simple Western lane view shows a specific band for GAPDH in 0.05 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Simple-Western-NB300-325-img0004.jpg)
![Western Blot: GAPDH Antibody [NB300-325] - GCNY-medicated serum decreased Nrf2 levels & inflammatory cytokines of NF-kappa B & IL-6. (A) Intracellular relative ROS levels in HMC-3 cells treated with different concentrations (0, 50, 100, & 200 μm) of H2O2 for 4 h. Auto-fluo refers to the auto-fluorescence intensity value of HMC-3 cells. (B,C) The mRNA & protein levels of Nrf2 were expressed by HMC-3 treated with different concentrations of H2O2. (D) The significantly elevated whole protein level in NC + H2O2 group cells indicates an increased proliferation of HMC-3 cells compared to the NC group cells & (E) ROS levels were attenuated in cells treated by GCNY-medicated serum. (F–H) Through automated Western blot analysis, GCNY group cells expressed lower levels of Nrf2, NF-kappa s p65, pNF-kappa B p65 (ser536), & IL-6 compared with NC + H2O2 group cells. ns, no significance, *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/36188472), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-325_rabbit-polyclonal-gapdh-antibody-310202416212345.jpg)
![Western Blot: GAPDH Antibody [NB300-325] - GCNY-medicated serum decreased Nrf2 levels & inflammatory cytokines of NF-kappa B & IL-6. (A) Intracellular relative ROS levels in HMC-3 cells treated with different concentrations (0, 50, 100, & 200 μm) of H2O2 for 4 h. Auto-fluo refers to the auto-fluorescence intensity value of HMC-3 cells. (B,C) The mRNA & protein levels of Nrf2 were expressed by HMC-3 treated with different concentrations of H2O2. (D) The significantly elevated whole protein level in NC + H2O2 group cells indicates an increased proliferation of HMC-3 cells compared to the NC group cells & (E) ROS levels were attenuated in cells treated by GCNY-medicated serum. (F–H) Through automated Western blot analysis, GCNY group cells expressed lower levels of Nrf2, NF-kappa s p65, pNF-kappa B p65 (ser536), & IL-6 compared with NC + H2O2 group cells. ns, no significance, *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/36188472), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-325_rabbit-polyclonal-gapdh-antibody-310202416212378.jpg)
![Western Blot: GAPDH Antibody [NB300-325] - GCNY-medicated serum decreased Nrf2 levels & inflammatory cytokines of NF-kappa B & IL-6. (A) Intracellular relative ROS levels in HMC-3 cells treated with different concentrations (0, 50, 100, & 200 μm) of H2O2 for 4 h. Auto-fluo refers to the auto-fluorescence intensity value of HMC-3 cells. (B,C) The mRNA & protein levels of Nrf2 were expressed by HMC-3 treated with different concentrations of H2O2. (D) The significantly elevated whole protein level in NC + H2O2 group cells indicates an increased proliferation of HMC-3 cells compared to the NC group cells & (E) ROS levels were attenuated in cells treated by GCNY-medicated serum. (F–H) Through automated Western blot analysis, GCNY group cells expressed lower levels of Nrf2, NF-kappa s p65, pNF-kappa B p65 (ser536), & IL-6 compared with NC + H2O2 group cells. ns, no significance, *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/36188472), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-325_rabbit-polyclonal-gapdh-antibody-310202416205140.jpg)
![Western Blot: GAPDH Antibody [NB300-325] - GCNY-medicated serum decreased Nrf2 levels & inflammatory cytokines of NF-kappa B & IL-6. (A) Intracellular relative ROS levels in HMC-3 cells treated with different concentrations (0, 50, 100, & 200 μm) of H2O2 for 4 h. Auto-fluo refers to the auto-fluorescence intensity value of HMC-3 cells. (B,C) The mRNA & protein levels of Nrf2 were expressed by HMC-3 treated with different concentrations of H2O2. (D) The significantly elevated whole protein level in NC + H2O2 group cells indicates an increased proliferation of HMC-3 cells compared to the NC group cells & (E) ROS levels were attenuated in cells treated by GCNY-medicated serum. (F–H) Through automated Western blot analysis, GCNY group cells expressed lower levels of Nrf2, NF-kappa s p65, pNF-kappa B p65 (ser536), & IL-6 compared with NC + H2O2 group cells. ns, no significance, *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/36188472), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-325_rabbit-polyclonal-gapdh-antibody-31020241621812.jpg)