| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54790SS | |||
| Azide and BSA Free | NBP2-80644 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33819-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56521SS | |||
| Azide and BSA Free | NBP2-33216 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-71691SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76854-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52716-0.025mg | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP1-51140B | |||
![Western Blot: CD36 Antibody (1283D) [NBP2-54790] - Western blot shows lysates of human placenta tissue and human platelets. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human CD36 Monoclonal antibody (catalog number NBP2-54790) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). A specific band was detected for CD36/SR-B3 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/CD36-SR-B3-Antibody-1283D-Western-Blot-NBP2-54790-img0023.jpg "Western Blot: CD36 Antibody (1283D) [NBP2-54790] - Western blot shows lysates of human placenta tissue and human platelets. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human CD36 Monoclonal antibody (catalog number NBP2-54790) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). A specific band was detected for CD36/SR-B3 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions.")
![Immunohistochemistry-Paraffin: CD36 Antibody (1283D) [NBP2-54790] - CD36 was detected in immersion fixed paraffin-embedded sections of human heart using Rabbit Anti-Human CD36/SR-B3 Monoclonal Antibody for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte(TM) HRP Polymer Antibody. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cardiomyocyte membranes.](http://images.novusbio.com/fullsize/CD36-SR-B3-Antibody-1283D-Immunohistochemistry-Paraffin-NBP2-54790-img0002.jpg)
![Western Blot: CD36 Antibody (1283D) [NBP2-54790] - Total protein from human skin and adipose tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-CD36 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/CD36-SR-B3-Antibody-1283D-Western-Blot-NBP2-54790-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD36 Antibody (1283D) [NBP2-54790] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-CD36 (1283D) at 5.0 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective..](http://images.novusbio.com/fullsize/CD36-SR-B3-Antibody-1283D-Immunocytochemistry-Immunofluorescence-NBP2-54790-img0003.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - 3T3-L1 adipocytes were pretreated and treated with ML348 (10 uM) for 1 h, followed by BSA or oleate treatment for another 1 h. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD36-Antibody-1283D-BSA-Free-Western-Blot-NBP2-54790-img0024.jpg)
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - LYN phosphorylates DHHC5 & is required for the endocytosis of FAs.a 3T3-L1 adipocytes pretreated with serum-free medium for 4 h & PP2 (15 μM) for 1 h, followed by treatment with BSA or oleate (100 μM) for 5 min. Cells harvested & immunoprecipitated with anti-DHHC5 antibody to detect phosphorylation of Tyr91. b 3T3-L1 adipocytes transduced with indicated shRNA set up & treated with oleate for 5 min. Cells harvested to detect Tyr91 phosphorylation as in (a). c On day 0, HEK293T cells set up at 2.5 × 105 cells per 6-cm dish. On day 2, cells transfected with 0.5 μg of WT, Y91E, or Y91F of DHHC5-Flag/pCDH-puro and/or 0.5 μg LYN/pCDNA3.3. On day 3, cells harvested & immunoprecipitated with anti-Flag M2 beads to detect phosphorylation of DHHC5 (pY). Control & LYN knockdown adipocytes pretreated & treated with BSA or oleate (100 μM) for 1 h, followed by immunostaining (d), surface biotinylation (e), & Acyl-RAC (f) assays. Control & CD36 knockdown 3T3-L1 adipocytes pretreated & treated with oleate (100 μM) for indicated time (g) or 5 min (h). Cells harvested & subjected to immunoprecipitation of LYN to detect phosphorylation of LYN (Y396). i, j WT & Cd36−/− SVFs set up & subjected to FA uptake as in Fig. 3h, i, except that cells pretreated with PP2 (15 μM). Each value represents mean ± SEM obtained from 20 cells. Two-sided Student’s t test was performed between DMSO & PP2 treated cells. k WT & Cd36−/− adipocytes set up & subjected to 3H-oleate uptake as in Fig. 3j, except that cells pretreated with PP2 (15 μM). Each value represents mean ± SEM obtained from 3samples. Two-sided Student’s t test was performed between DMSO & PP2 treated cells. These experiments repeated at least twice. Source data provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-3102024161365.jpg)
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - LYN phosphorylates DHHC5 & is required for the endocytosis of FAs.a 3T3-L1 adipocytes pretreated with serum-free medium for 4 h & PP2 (15 μM) for 1 h, followed by treatment with BSA or oleate (100 μM) for 5 min. Cells harvested & immunoprecipitated with anti-DHHC5 antibody to detect phosphorylation of Tyr91. b 3T3-L1 adipocytes transduced with indicated shRNA set up & treated with oleate for 5 min. Cells harvested to detect Tyr91 phosphorylation as in (a). c On day 0, HEK293T cells set up at 2.5 × 105 cells per 6-cm dish. On day 2, cells transfected with 0.5 μg of WT, Y91E, or Y91F of DHHC5-Flag/pCDH-puro and/or 0.5 μg LYN/pCDNA3.3. On day 3, cells harvested & immunoprecipitated with anti-Flag M2 beads to detect phosphorylation of DHHC5 (pY). Control & LYN knockdown adipocytes pretreated & treated with BSA or oleate (100 μM) for 1 h, followed by immunostaining (d), surface biotinylation (e), & Acyl-RAC (f) assays. Control & CD36 knockdown 3T3-L1 adipocytes pretreated & treated with oleate (100 μM) for indicated time (g) or 5 min (h). Cells harvested & subjected to immunoprecipitation of LYN to detect phosphorylation of LYN (Y396). i, j WT & Cd36−/− SVFs set up & subjected to FA uptake as in Fig. 3h, i, except that cells pretreated with PP2 (15 μM). Each value represents mean ± SEM obtained from 20 cells. Two-sided Student’s t test was performed between DMSO & PP2 treated cells. k WT & Cd36−/− adipocytes set up & subjected to 3H-oleate uptake as in Fig. 3j, except that cells pretreated with PP2 (15 μM). Each value represents mean ± SEM obtained from 3samples. Two-sided Student’s t test was performed between DMSO & PP2 treated cells. These experiments repeated at least twice. Source data provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-310202416114783.jpg)
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - FAs trigger internalization of CD36.a, b On day 8 of differentiation, 3T3-L1 adipocytes were pretreated with serum-free medium for 4 h, & then treated with BSA-conjugated oleate (100 μM) for indicated time. a One set of cells was subjected to immunostaining with anti-CD36 & anti-CAV1 antibodies. LipidTOX was used to label lipid droplets. Images were taken under a Zeiss LSM-780 microscope in a 3D Z-stack mode & reconstructed using Imaris 9.2.0. b The other set of cells was subjected to surface biotinylation assay & blotted with indicated antibodies. c, d On day 4 of differentiation, 3T3-L1 cells were infected with lentivirus encoding scrambled shRNA or shRNAs against CD36 or CAV1. On day 5, cells were selected with 5 μg/ml puromycin. On day 8, cells were pretreated as in (a) & treated with oleate (100 μM) for 4 h, followed by immunostaining with anti-CD36 & anti-CAV1 antibodies (c), or surface biotinylation assay (d). e, f 3T3-L1 adipocytes were pretreated as in (a) & treated with BSA-conjugated FAs with different chain lengths or saturation (100 μM) for 4 h. Cells were subjected to immunostaining with anti-CD36 antibody (e), or surface biotinylation assay (f). After oleate treatment for 4 h, 3T3-L1 adipocytes were switched to serum-free medium for indicated time & harvested for immunostaining (g) & surface biotinylation (h). The scale bars were as indicated in each figure. These experiments were repeated at least three times. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-310202416121990.jpg)
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - FAs trigger internalization of CD36.a, b On day 8 of differentiation, 3T3-L1 adipocytes were pretreated with serum-free medium for 4 h, & then treated with BSA-conjugated oleate (100 μM) for indicated time. a One set of cells was subjected to immunostaining with anti-CD36 & anti-CAV1 antibodies. LipidTOX was used to label lipid droplets. Images were taken under a Zeiss LSM-780 microscope in a 3D Z-stack mode & reconstructed using Imaris 9.2.0. b The other set of cells was subjected to surface biotinylation assay & blotted with indicated antibodies. c, d On day 4 of differentiation, 3T3-L1 cells were infected with lentivirus encoding scrambled shRNA or shRNAs against CD36 or CAV1. On day 5, cells were selected with 5 μg/ml puromycin. On day 8, cells were pretreated as in (a) & treated with oleate (100 μM) for 4 h, followed by immunostaining with anti-CD36 & anti-CAV1 antibodies (c), or surface biotinylation assay (d). e, f 3T3-L1 adipocytes were pretreated as in (a) & treated with BSA-conjugated FAs with different chain lengths or saturation (100 μM) for 4 h. Cells were subjected to immunostaining with anti-CD36 antibody (e), or surface biotinylation assay (f). After oleate treatment for 4 h, 3T3-L1 adipocytes were switched to serum-free medium for indicated time & harvested for immunostaining (g) & surface biotinylation (h). The scale bars were as indicated in each figure. These experiments were repeated at least three times. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-31020241612242.jpg)
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - FAs trigger internalization of CD36.a, b On day 8 of differentiation, 3T3-L1 adipocytes were pretreated with serum-free medium for 4 h, & then treated with BSA-conjugated oleate (100 μM) for indicated time. a One set of cells was subjected to immunostaining with anti-CD36 & anti-CAV1 antibodies. LipidTOX was used to label lipid droplets. Images were taken under a Zeiss LSM-780 microscope in a 3D Z-stack mode & reconstructed using Imaris 9.2.0. b The other set of cells was subjected to surface biotinylation assay & blotted with indicated antibodies. c, d On day 4 of differentiation, 3T3-L1 cells were infected with lentivirus encoding scrambled shRNA or shRNAs against CD36 or CAV1. On day 5, cells were selected with 5 μg/ml puromycin. On day 8, cells were pretreated as in (a) & treated with oleate (100 μM) for 4 h, followed by immunostaining with anti-CD36 & anti-CAV1 antibodies (c), or surface biotinylation assay (d). e, f 3T3-L1 adipocytes were pretreated as in (a) & treated with BSA-conjugated FAs with different chain lengths or saturation (100 μM) for 4 h. Cells were subjected to immunostaining with anti-CD36 antibody (e), or surface biotinylation assay (f). After oleate treatment for 4 h, 3T3-L1 adipocytes were switched to serum-free medium for indicated time & harvested for immunostaining (g) & surface biotinylation (h). The scale bars were as indicated in each figure. These experiments were repeated at least three times. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-310202416114740.jpg)
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - FAs trigger internalization of CD36.a, b On day 8 of differentiation, 3T3-L1 adipocytes were pretreated with serum-free medium for 4 h, & then treated with BSA-conjugated oleate (100 μM) for indicated time. a One set of cells was subjected to immunostaining with anti-CD36 & anti-CAV1 antibodies. LipidTOX was used to label lipid droplets. Images were taken under a Zeiss LSM-780 microscope in a 3D Z-stack mode & reconstructed using Imaris 9.2.0. b The other set of cells was subjected to surface biotinylation assay & blotted with indicated antibodies. c, d On day 4 of differentiation, 3T3-L1 cells were infected with lentivirus encoding scrambled shRNA or shRNAs against CD36 or CAV1. On day 5, cells were selected with 5 μg/ml puromycin. On day 8, cells were pretreated as in (a) & treated with oleate (100 μM) for 4 h, followed by immunostaining with anti-CD36 & anti-CAV1 antibodies (c), or surface biotinylation assay (d). e, f 3T3-L1 adipocytes were pretreated as in (a) & treated with BSA-conjugated FAs with different chain lengths or saturation (100 μM) for 4 h. Cells were subjected to immunostaining with anti-CD36 antibody (e), or surface biotinylation assay (f). After oleate treatment for 4 h, 3T3-L1 adipocytes were switched to serum-free medium for indicated time & harvested for immunostaining (g) & surface biotinylation (h). The scale bars were as indicated in each figure. These experiments were repeated at least three times. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-310202416114741.jpg)
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - CD36-mediated caveolar endocytosis transports FAs into cells.a–c 3T3-L1 adipocytes were pretreated with serum-free medium for 4 h, & then treated with BSA-conjugated PacFA (50 μM) for 20 min, followed by UV crosslinking on ice for 30 min. a, b Cells were subjected to click chemistry using an N3-Alexa Fluro 488, & immunostained with anti-CD36 antibody. Colocalization of PacFA & CD36 was quantified from 24 cells over three independent experiments & plotted in (b). The value represents mean ± SEM. c Cells were lysed & subjected to click chemistry assay using N3-biotin. PacFA-labeled proteins were captured with streptavidin beads & subjected to western blot using anti-CD36 & anti-FABP4 antibodies. d WT, Cav1−/−, Cd36−/−, & Cav1−/−;Cd36−/− SVFs were isolated & differentiated into adipocytes & treated with 100 μM 3H-oleate (specific activity, 2268 dpm/nmol) for 1 h. Lipid fractions were extracted from the cells & subjected to scintillation counting. The radioactive counting was normalized to protein content. Each value represents mean ± SEM obtained from three samples. Two-sided Student’s t test was performed between WT & each of the knockout cells. These experiments were repeated twice. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-310202416113520.jpg)
![Western Blot: CXCL8/IL-8 Antibody [NBP2-33819] - Analysis in human cell line EFO-21.](http://images.novusbio.com/fullsize/CXCL8-IL-8-Antibody-Western-Blot-NBP2-33819-img0005.jpg "Western Blot: CXCL8/IL-8 Antibody [NBP2-33819] - Analysis in human cell line EFO-21.")
![Immunocytochemistry/Immunofluorescence: CXCL8/IL-8 Antibody [NBP2-33819] - Staining of human cell line U-251 MG shows localization to the Golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CXCL8-IL-8-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33819-img0006.jpg)
![Immunohistochemistry-Paraffin: CXCL8/IL-8 Antibody [NBP2-33819] - Staining of human rectum shows moderate positivity in plasma.](http://images.novusbio.com/fullsize/CXCL8-IL-8-Antibody-Immunohistochemistry-Paraffin-NBP2-33819-img0010.jpg)
![Immunohistochemistry-Paraffin: CXCL8/IL-8 Antibody [NBP2-33819] - Staining of human fallopian tube shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/CXCL8-IL-8-Antibody-Immunohistochemistry-Paraffin-NBP2-33819-img0007.jpg)
![Immunohistochemistry-Paraffin: CXCL8/IL-8 Antibody [NBP2-33819] - Staining of human lymph node shows weak to moderate cytoplasmic positivity in a subset of non-germinal center cells.](http://images.novusbio.com/fullsize/CXCL8-IL-8-Antibody-Immunohistochemistry-Paraffin-NBP2-33819-img0008.jpg)
![Immunohistochemistry-Paraffin: CXCL8/IL-8 Antibody [NBP2-33819] - Staining of human placenta shows moderate positivity in plasma.](http://images.novusbio.com/fullsize/CXCL8-IL-8-Antibody-Immunohistochemistry-Paraffin-NBP2-33819-img0009.jpg)
![Western Blot: IRF6 Antibody [NBP1-51911] - HEK293 overexpressing IRF6 and probed with (mock transfection in first lane).](http://images.novusbio.com/fullsize/IRF6-Antibody-Western-Blot-NBP1-51911-img0003.jpg "Western Blot: IRF6 Antibody [NBP1-51911] - HEK293 overexpressing IRF6 and probed with (mock transfection in first lane).")
![Immunohistochemistry-Paraffin: IRF6 Antibody [NBP1-51911] - Staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/IRF6-Antibody-Immunohistochemistry-Paraffin-NBP1-51911-img0006.jpg)
![Western Blot: IRF6 Antibody [NBP1-51911] - (0.2ug/ml) of Human Ovary lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.](http://images.novusbio.com/fullsize/IRF6-Antibody-Western-Blot-NBP1-51911-img0001.jpg)
![Immunohistochemistry-Paraffin: IRF6 Antibody [NBP1-51911] - staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/IRF6-Antibody-Immunohistochemistry-Paraffin-NBP1-51911-img0004.jpg)
![Western Blot: TLR2 Antibody - Azide Free [NBP2-24861] - Analysis of TLR2 in transfected cell lysate using this antibody.](http://images.novusbio.com/fullsize/TLR2-Antibody---Azide-Free-Western-Blot-NBP2-24861-img0001.jpg "Western Blot: TLR2 Antibody - Azide Free [NBP2-24861] - Analysis of TLR2 in transfected cell lysate using this antibody.")
![Immunohistochemistry: TLR2 Antibody - Azide Free [NBP2-24861] - Analysis of mouse pancreas tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml. Image using the standard format of this product.](http://images.novusbio.com/fullsize/TLR2-Antibody---Azide-Free-Immunohistochemistry-NBP2-24861-img0003.jpg)
![Immunohistochemistry: TLR2 Antibody - Azide Free [NBP2-24861] - Representative photographs of immunohistochemistry for tubular TLR2 in G) ctrl, H) ctrl + empa, I) diabetic (dm), J) dm + empagliflozin, K) dm + telmisartan groups (Magnification = original X 200) and quantification of tubular TLR2 expression by Image J (L). (Data are expressed as mean +/- SEM with *=P<0.05 vs ctrl, **=P<0.001 vs ctrl and #=P<0.05 vs dm). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0108994) licensed under a CC-BY license. Image using the standard format of this product.](http://images.novusbio.com/fullsize/TLR2-Antibody---Azide-Free-Immunohistochemistry-NBP2-24861-img0002.jpg)
![Western Blot: PU.1/Spi-1 Antibody [NBP2-27163] - PU.1/Spi1 Antibody [NBP2-27163] - Analysis of PU.1/Spi1 in U937 cell lysate in the 1) absence and 2) presence of immunizing peptide using NBP2-27163.](http://images.novusbio.com/fullsize/PU.1-Spi-1-Antibody-Western-Blot-NBP2-27163-img0005.jpg "Western Blot: PU.1/Spi-1 Antibody [NBP2-27163] - PU.1/Spi1 Antibody [NBP2-27163] - Analysis of PU.1/Spi1 in U937 cell lysate in the 1) absence and 2) presence of immunizing peptide using NBP2-27163.")
![<b>Biological Strategies Validation. </b>Flow Cytometry: PU.1/Spi-1 Antibody [NBP2-27163] - PU.1/Spi1 Antibody [NBP2-27163] - Intracellular analysis of PU.1 in mouse RAW cells (top) and PMA treated (20 ng/ml, overnight) human ThP1 cells (bottom) using 1.25 ug/10^6 cells. Green peak represents isotype control this antibody ; red represents anti-PU.1 antibody.](http://images.novusbio.com/fullsize/PU.1-Spi-1-Antibody-Flow-Cytometry-NBP2-27163-img0006.jpg)
![Western Blot: PU.1/Spi1 Antibody [NBP2-27163] - analysis of 293T cell lysate (30ug) using anti-PU.1/Spi1 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/PU.1-Spi1-Antibody-Western-Blot-NBP2-27163-img0003.jpg)
![Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody [NBP2-16025] - Human gastric cancer, using CTGF antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/CTGF-CCN2-Antibody-Immunohistochemistry-Paraffin-NBP2-16025-img0007.jpg "Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody [NBP2-16025] - Human gastric cancer, using CTGF antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: CTGF/CCN2 Antibody [NBP2-16025] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CTGF antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CTGF-CCN2-Antibody-Western-Blot-NBP2-16025-img0011.jpg)
![Western Blot: DNMT3A Antibody (64B814.1) [NB100-56521] - Analysis of Dnmt3a in (A) Dnmt3a transfected and (B) untransfected 293 cell lysate using Dnmt3a antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/DNMT3A-Antibody-64B814-1-Western-Blot-NB100-56521-img0005.jpg "Western Blot: DNMT3A Antibody (64B814.1) [NB100-56521] - Analysis of Dnmt3a in (A) Dnmt3a transfected and (B) untransfected 293 cell lysate using Dnmt3a antibody at 1 ug/ml.")
![Flow (Intracellular): DNMT3A Antibody (64B814.1) [NB100-56521] - Analysis of Alexa Fluor (R) 488 conjugate of NB100-56521. An intracellular stain was performed on HeLa cells with DNMT3A antibody (64B814.1) NB100-56521AF488 (blue) and a matched isotype control NBP2-27287AF488 (orange).](http://images.novusbio.com/fullsize/DNMT3A-Antibody-64B814-1-Flow-Intracellular-NB100-56521-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Dicer Antibody [NBP1-71691] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Dicer at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Dicer-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-71691-img0008.jpg "Immunocytochemistry/Immunofluorescence: Dicer Antibody [NBP1-71691] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Dicer at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Immunohistochemistry-Paraffin: Dicer Antibody [NBP1-71691] - Analysis of Dicer in paraffin embedded human colorectal cancer tissue using anti-Dicer antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Dicer-Antibody-Immunohistochemistry-Paraffin-NBP1-71691-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Dicer Antibody [NBP1-71691] - Dicer antibody was tested in HeLa cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Dicer-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-71691-img0005.jpg)
![Immunohistochemistry: Dicer Antibody [NBP1-71691] - Staining of Dicer in mouse prostate using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Dicer-Antibody-Immunohistochemistry-NBP1-71691-img0006.jpg)
![Western Blot: ZBP1/DLM-1/DAI Antibody [NBP1-76854] - Mouse small intestine tissue lysate with Z DNA binding protein antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.](http://images.novusbio.com/fullsize/ZBP1-DLM-1-DAI-Antibody-Western-Blot-NBP1-76854-img0001.jpg "Western Blot: ZBP1/DLM-1/DAI Antibody [NBP1-76854] - Mouse small intestine tissue lysate with Z DNA binding protein antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.")
![Immunohistochemistry-Paraffin: ZBP1/DLM-1/DAI Antibody [NBP1-76854] - Human small intestine tissue with Z DNA binding protein antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/ZBP1-DLM-1-DAI-Antibody-Immunohistochemistry-Paraffin-NBP1-76854-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: ZBP1/DLM-1/DAI Antibody - BSA Free [NBP1-76854] - Immunofluorescence of ZBP1/DLM-1/DAI in Human Small Intestine tissue with ZBP1/DLM-1/DAI antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76854_rabbit-polyclonal-zbp1-dlm-1-dai-antibody-2711202420432396.jpg)
![ZBP1 is mainly expressed in macrophages and alters the ratio of M1 and M2 macrophages in sepsis‐induced myocardial dysfunction. A single‐nucleus mRNA sequencing (snRNA‐seq) was performed in hearts from LPS‐treated WT or Zbp1−/− mice (for each group, single‐cell suspensions from three hearts were pooled as one sample). (A) UMAP plot of cell clusters in myocardial tissues from WT and Zbp1−/− mice induced by LPS. CM, cardiomyocyte; EC, endothelial cell; FB, fibroblast; Mac, macrophage; NP, neutrophil. (B,C) UMAP plot and dot plot of Zbp1 expression in myocardial tissues from WT and Zbp1−/− mice induced by LPS. (D,E) UMAP plot of macrophage in myocardial tissues from WT and Zbp1−/− mice induced by LPS. M1, M1 macrophage; M2, M2 macrophage. (F–H) Heart tissues were labelled with M1 macrophage marker iNOS and M2 macrophage marker CD206. Tissues were counterstained with DAPI. Representative staining images (F) and quantification of positive cells (G,H) are shown [scale bar = 50 um, n = 5]. Mean +/- SEM; *P < 0.05, ***P < 0.001, ns, no significance. WT, wild‐type; ZBP1, Z‐DNA binding protein 1; LPS, lipopolysaccharide; snRNA‐seq, single‐nucleus mRNA sequencing. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/40289345), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-76854_rabbit-polyclonal-zbp1-dlm-1-dai-antibody-immunocytochemistry-immunofluorescence-13220261920717.jpg)
![Western Blot: IRAK4 Antibody (2H9) [NBP2-37575] - Analysis using IRAK4 mouse mAb against THP-1 (1), Hela (2), K562 (3), MCF-7 (4), RAW264.7 (5), Jurkat (6) and Cos7 (7) cell lysate.](http://images.novusbio.com/fullsize/IRAK4-Antibody-2H9-Western-Blot-NBP2-37575-img0003.jpg "Western Blot: IRAK4 Antibody (2H9) [NBP2-37575] - Analysis using IRAK4 mouse mAb against THP-1 (1), Hela (2), K562 (3), MCF-7 (4), RAW264.7 (5), Jurkat (6) and Cos7 (7) cell lysate.")
![Immunohistochemistry-Paraffin: IRAK4 Antibody (2H9) [NBP2-37575] - Analysis of human kidney cancer tissues using IRAK4 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/IRAK4-Antibody-2H9-Immunohistochemistry-NBP2-37575-img0006.jpg)
![Flow Cytometry: IRAK4 Antibody (2H9) [NBP2-37575] - Analysis of Hela cells using IRAK4 mouse mAb (blue) and negative control (red).](http://images.novusbio.com/fullsize/IRAK4-Antibody-2H9-Flow-Cytometry-NBP2-37575-img0004.jpg)
![Western Blot: IRAK4 Antibody (2H9) [NBP2-37575] - Analysis using IRAK4 mAb against human IRAK4 (AA: 21-198) recombinant protein. (Expected MW is 45.4 kDa)](http://images.novusbio.com/fullsize/IRAK4-Antibody-2H9-Western-Blot-NBP2-37575-img0002.jpg)
![Immunohistochemistry-Paraffin: IRAK4 Antibody (2H9) [NBP2-37575] - Analysis of human lung cancer tissues using IRAK4 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/IRAK4-Antibody-2H9-Immunohistochemistry-NBP2-37575-img0005.jpg)
![ELISA: IRAK4 Antibody (2H9) [NBP2-37575] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/IRAK4-Antibody-2H9-ELISA-NBP2-37575-img0001.jpg)
![Flow Cytometry: Complement Component C1qA Antibody (JL-1) [NBP1-51139] - Intracellular flow cytometry for C1q in peritoneal macrophages. Anti-C1q was conjugated to PE using a Lightning Link kit for use in flow cytometry. Macrophages are gated on CD45+CD11b+ and T cells are gated on CD45+CD3+ lymphocytes. Dead cells were excluded, and Fc receptors were blocked to prevent non-specific interactions. This is image was submitted via customer review.](http://images.novusbio.com/fullsize/Complement-Component-C1qA-Antibody-JL-1-Flow-Cytometry-NBP1-51139-img0002.jpg "Flow Cytometry: Complement Component C1qA Antibody (JL-1) [NBP1-51139] - Intracellular flow cytometry for C1q in peritoneal macrophages. Anti-C1q was conjugated to PE using a Lightning Link kit for use in flow cytometry. Macrophages are gated on CD45+CD11b+ and T cells are gated on CD45+CD3+ lymphocytes. Dead cells were excluded, and Fc receptors were blocked to prevent non-specific interactions. This is image was submitted via customer review.")
