| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-49891-0.1mg | |||
| BSA Free | NBP2-49891-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-152SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54790SS | |||
| Azide and BSA Free | NBP2-80644 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54582-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97662-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54405-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24539SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76480-0.025mg | |||
![Flow Cytometry: CD3 Antibody (CA17.2A12) [NBP2-49891] - Figure A. Purified Rat anti Canine CD90 detected with Goat anti Rat IgG2a DyLight 649 and Mouse IgG1 FITC isotype control. Figure B. Purified Rat anti Canine CD90 detected with Goat anti Rat IgG2a PE and Mouse anti Canine CD3 FITC.](http://images.novusbio.com/fullsize/CD3-Antibody-CA17-2A12-Flow-Cytometry-NBP2-49891-img0009.jpg "Flow Cytometry: CD3 Antibody (CA17.2A12) [NBP2-49891] - Figure A. Purified Rat anti Canine CD90 detected with Goat anti Rat IgG2a DyLight 649 and Mouse IgG1 FITC isotype control. Figure B. Purified Rat anti Canine CD90 detected with Goat anti Rat IgG2a PE and Mouse anti Canine CD3 FITC.")
![Flow Cytometry: CD3 Antibody (CA17.2A12) [NBP2-49891] - Figure A: APC conjugated rat anti-Canine CD4 and FITC conjugated mouse IgG1 isotype control. Figure B: APC conjugated rat anti-Canine CD4 and FITC conjugated mouse anti-Canine CD3.](http://images.novusbio.com/fullsize/CD3-Antibody-CA17-2A12-Flow-Cytometry-NBP2-49891-img0001.jpg)
![Flow Cytometry: CD3 Antibody (CA17.2A12) [NBP2-49891] - Figure A: RPE conjugated Rat anti-Dog CD4 and Mouse IgG1 isotype control labeled with Goat anti-Mouse FITC. Figure B: RPE conjugated Rat anti-Dog CD4 and Mouse anti-Dog CD3 labeled with Goat anti Mouse DyLight FITC.](http://images.novusbio.com/fullsize/CD3-Antibody-CA17-2A12-Flow-Cytometry-NBP2-49891-img0002.jpg)
![Flow Cytometry: CD3 Antibody (CA17.2A12) [NBP2-49891] - Figure A. Alexa Fluor 700 conjugated Rat anti Canine CD8 and FITC conjugated Mouse IgG1 isotype control. Figure B. Alexa Fluor 700 conjugated Rat anti Canine CD8 and FITC conjugated Mouse anti Canine CD3.](http://images.novusbio.com/fullsize/CD3-Antibody-CA17-2A12-Flow-Cytometry-NBP2-49891-img0003.jpg)
![Flow Cytometry: CD3 Antibody (CA17.2A12) [NBP2-49891] - Figure A. FITC conjugated Mouse anti Canine CD3 and RPE conjugated Rat IgG2a isotype control. Figure B. FITC conjugated Mouse anti Canine CD3 and RPE conjugated Rat anti Canine CD4.](http://images.novusbio.com/fullsize/CD3-Antibody-CA17-2A12-Flow-Cytometry-NBP2-49891-img0004.jpg)
![Flow Cytometry: CD3 Antibody (CA17.2A12) [NBP2-49891] - Figure A. RPE conjugated Rat anti Canine CD4 and FITC conjugated Mouse IgG1 isotype control. Figure B. RPE conjugated Rat anti Canine CD4 and FITC conjugated Mouse anti Canine CD3.](http://images.novusbio.com/fullsize/CD3-Antibody-CA17-2A12-Flow-Cytometry-NBP2-49891-img0005.jpg)
![Flow Cytometry: CD3 Antibody (CA17.2A12) [NBP2-49891] - Figure A. RPE conjugated Rat anti Canine CD8 and FITC conjugated Mouse IgG1 isotype control. Figure B. RPE conjugated Rat anti Canine CD8 and FITC conjugated Mouse anti Canine CD3.](http://images.novusbio.com/fullsize/CD3-Antibody-CA17-2A12-Flow-Cytometry-NBP2-49891-img0006.jpg)
![Flow Cytometry: CD3 Antibody (CA17.2A12) [NBP2-49891] - Figure A. FITC conjugated Mouse anti Canine CD3 and RPE conjugated Rat IgG1 isotype control. Figure B. FITC conjugated Mouse anti Canine CD3 and RPE conjugated Rat anti Canine CD8.](http://images.novusbio.com/fullsize/CD3-Antibody-CA17-2A12-Flow-Cytometry-NBP2-49891-img0007.jpg)
![Flow Cytometry: CD3 Antibody (CA17.2A12) [NBP2-49891] - Figure A. FITC conjugated Mouse anti Canine CD3 and purified Rat IgG2a isotype control detected with Goat anti Rat IgG2a DyLight 649. Figure B. FITC conjugated Mouse anti Canine CD3 and purified Mouse anti Canine CD90 detected with Goat anti Rat IgG2a DyLight 649.](http://images.novusbio.com/fullsize/CD3-Antibody-CA17-2A12-Flow-Cytometry-NBP2-49891-img0008.jpg)
![Western Blot: Cathepsin E Antibody [NB400-152] - Detection of Cathepsin E in 10 ug of murine reticulocyte lysate using NB400-152.](http://images.novusbio.com/fullsize/Cathepsin-E-Antibody-Western-Blot-NB400-152-img0005.jpg "Western Blot: Cathepsin E Antibody [NB400-152] - Detection of Cathepsin E in 10 ug of murine reticulocyte lysate using NB400-152.")
![Immunohistochemistry: Cathepsin E Antibody [NB400-152] - Analysis of Cathepsin E in human renal cancer using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Cathepsin-E-Antibody-Immunohistochemistry-NB400-152-img0006.jpg)
![Western Blot: Cathepsin E Antibody [NB400-152] - Analysis of Cathepsin E on mouse stomach lysate.](http://images.novusbio.com/fullsize/Cathepsin-E-Antibody-Western-Blot-NB400-152-img0001.jpg)
![Western Blot: Cathepsin E Antibody [NB400-152] - The expression & methylation status of Ctse gene in CD4+ T cells isolated from MRL/lpr lupus-prone (MRL) & C57BL/6 (B6) mice. (A) mRNA expression of Ctse in CD4+, CD8+, B cells & macrophages in B6 & MRL mice. ***p < 0.001. (B) Western blot analyses of CTSE in CD4+, CD8+, B cells & macrophages in B6, MRL & mice. (C) Methylated region expanding 583 bp in Ctse intron 1 in B6 mice demonstrated by UCSC Genome Browser. In MRL mice, 583 bp region is hypomethylated. Duplicate reads are indicated by arrows. (D) Nucleotide sequence within 583 bp flanking region of Ctse intron 1 in B6 mice. Among 13 CpGs (red & underlined), 11th CpG (boxed) includes mCGCG binding motif for Kaiso. After genomic DNAs were bisulphite converted, amplified by PCR using Primer F1 & R1, subcloned, & isolated clones sequenced. 98.6% of CpGs (71 out of 72 colonies) in B6 mice & 76.9% CpGs (50 of 65) in MRL mice were methylated. (E) The structure of Kaiso, SMRT (Silencing Mediator of Retinoic acid & Thyroid hormone receptors)/N-CoR (Nuclear hormone receptor Co-Repressor), & HDAC3 (Histone deacetylase 3) complex. BTB (for BR-C, ttk & bab), POZ (for Pox virus & Zinc finger) & C2H2 zinc-finger domains are shown. (F) DNA sequencing of bisulphite converted DNAs in Ctse intron 1 in B6 & MRL mice. Percentage of methylation of 11th CpG is shown (n = 5). ***p < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30816218), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb400-152_rabbit-polyclonal-cathepsin-e-antibody-310202416161819.jpg)
![Western Blot: CD36 Antibody (1283D) [NBP2-54790] - Western blot shows lysates of human placenta tissue and human platelets. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human CD36 Monoclonal antibody (catalog number NBP2-54790) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). A specific band was detected for CD36/SR-B3 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/CD36-SR-B3-Antibody-1283D-Western-Blot-NBP2-54790-img0023.jpg "Western Blot: CD36 Antibody (1283D) [NBP2-54790] - Western blot shows lysates of human placenta tissue and human platelets. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human CD36 Monoclonal antibody (catalog number NBP2-54790) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). A specific band was detected for CD36/SR-B3 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions.")
![Immunohistochemistry-Paraffin: CD36 Antibody (1283D) [NBP2-54790] - CD36 was detected in immersion fixed paraffin-embedded sections of human heart using Rabbit Anti-Human CD36/SR-B3 Monoclonal Antibody for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte(TM) HRP Polymer Antibody. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cardiomyocyte membranes.](http://images.novusbio.com/fullsize/CD36-SR-B3-Antibody-1283D-Immunohistochemistry-Paraffin-NBP2-54790-img0002.jpg)
![Western Blot: CD36 Antibody (1283D) [NBP2-54790] - Total protein from human skin and adipose tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-CD36 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/CD36-SR-B3-Antibody-1283D-Western-Blot-NBP2-54790-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD36 Antibody (1283D) [NBP2-54790] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-CD36 (1283D) at 5.0 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective..](http://images.novusbio.com/fullsize/CD36-SR-B3-Antibody-1283D-Immunocytochemistry-Immunofluorescence-NBP2-54790-img0003.jpg)
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - LYN phosphorylates DHHC5 & is required for the endocytosis of FAs.a 3T3-L1 adipocytes pretreated with serum-free medium for 4 h & PP2 (15 μM) for 1 h, followed by treatment with BSA or oleate (100 μM) for 5 min. Cells harvested & immunoprecipitated with anti-DHHC5 antibody to detect phosphorylation of Tyr91. b 3T3-L1 adipocytes transduced with indicated shRNA set up & treated with oleate for 5 min. Cells harvested to detect Tyr91 phosphorylation as in (a). c On day 0, HEK293T cells set up at 2.5 × 105 cells per 6-cm dish. On day 2, cells transfected with 0.5 μg of WT, Y91E, or Y91F of DHHC5-Flag/pCDH-puro and/or 0.5 μg LYN/pCDNA3.3. On day 3, cells harvested & immunoprecipitated with anti-Flag M2 beads to detect phosphorylation of DHHC5 (pY). Control & LYN knockdown adipocytes pretreated & treated with BSA or oleate (100 μM) for 1 h, followed by immunostaining (d), surface biotinylation (e), & Acyl-RAC (f) assays. Control & CD36 knockdown 3T3-L1 adipocytes pretreated & treated with oleate (100 μM) for indicated time (g) or 5 min (h). Cells harvested & subjected to immunoprecipitation of LYN to detect phosphorylation of LYN (Y396). i, j WT & Cd36−/− SVFs set up & subjected to FA uptake as in Fig. 3h, i, except that cells pretreated with PP2 (15 μM). Each value represents mean ± SEM obtained from 20 cells. Two-sided Student’s t test was performed between DMSO & PP2 treated cells. k WT & Cd36−/− adipocytes set up & subjected to 3H-oleate uptake as in Fig. 3j, except that cells pretreated with PP2 (15 μM). Each value represents mean ± SEM obtained from 3samples. Two-sided Student’s t test was performed between DMSO & PP2 treated cells. These experiments repeated at least twice. Source data provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-3102024161365.jpg)
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - LYN phosphorylates DHHC5 & is required for the endocytosis of FAs.a 3T3-L1 adipocytes pretreated with serum-free medium for 4 h & PP2 (15 μM) for 1 h, followed by treatment with BSA or oleate (100 μM) for 5 min. Cells harvested & immunoprecipitated with anti-DHHC5 antibody to detect phosphorylation of Tyr91. b 3T3-L1 adipocytes transduced with indicated shRNA set up & treated with oleate for 5 min. Cells harvested to detect Tyr91 phosphorylation as in (a). c On day 0, HEK293T cells set up at 2.5 × 105 cells per 6-cm dish. On day 2, cells transfected with 0.5 μg of WT, Y91E, or Y91F of DHHC5-Flag/pCDH-puro and/or 0.5 μg LYN/pCDNA3.3. On day 3, cells harvested & immunoprecipitated with anti-Flag M2 beads to detect phosphorylation of DHHC5 (pY). Control & LYN knockdown adipocytes pretreated & treated with BSA or oleate (100 μM) for 1 h, followed by immunostaining (d), surface biotinylation (e), & Acyl-RAC (f) assays. Control & CD36 knockdown 3T3-L1 adipocytes pretreated & treated with oleate (100 μM) for indicated time (g) or 5 min (h). Cells harvested & subjected to immunoprecipitation of LYN to detect phosphorylation of LYN (Y396). i, j WT & Cd36−/− SVFs set up & subjected to FA uptake as in Fig. 3h, i, except that cells pretreated with PP2 (15 μM). Each value represents mean ± SEM obtained from 20 cells. Two-sided Student’s t test was performed between DMSO & PP2 treated cells. k WT & Cd36−/− adipocytes set up & subjected to 3H-oleate uptake as in Fig. 3j, except that cells pretreated with PP2 (15 μM). Each value represents mean ± SEM obtained from 3samples. Two-sided Student’s t test was performed between DMSO & PP2 treated cells. These experiments repeated at least twice. Source data provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-310202416114783.jpg)
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - FAs trigger internalization of CD36.a, b On day 8 of differentiation, 3T3-L1 adipocytes were pretreated with serum-free medium for 4 h, & then treated with BSA-conjugated oleate (100 μM) for indicated time. a One set of cells was subjected to immunostaining with anti-CD36 & anti-CAV1 antibodies. LipidTOX was used to label lipid droplets. Images were taken under a Zeiss LSM-780 microscope in a 3D Z-stack mode & reconstructed using Imaris 9.2.0. b The other set of cells was subjected to surface biotinylation assay & blotted with indicated antibodies. c, d On day 4 of differentiation, 3T3-L1 cells were infected with lentivirus encoding scrambled shRNA or shRNAs against CD36 or CAV1. On day 5, cells were selected with 5 μg/ml puromycin. On day 8, cells were pretreated as in (a) & treated with oleate (100 μM) for 4 h, followed by immunostaining with anti-CD36 & anti-CAV1 antibodies (c), or surface biotinylation assay (d). e, f 3T3-L1 adipocytes were pretreated as in (a) & treated with BSA-conjugated FAs with different chain lengths or saturation (100 μM) for 4 h. Cells were subjected to immunostaining with anti-CD36 antibody (e), or surface biotinylation assay (f). After oleate treatment for 4 h, 3T3-L1 adipocytes were switched to serum-free medium for indicated time & harvested for immunostaining (g) & surface biotinylation (h). The scale bars were as indicated in each figure. These experiments were repeated at least three times. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-310202416121990.jpg)
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - FAs trigger internalization of CD36.a, b On day 8 of differentiation, 3T3-L1 adipocytes were pretreated with serum-free medium for 4 h, & then treated with BSA-conjugated oleate (100 μM) for indicated time. a One set of cells was subjected to immunostaining with anti-CD36 & anti-CAV1 antibodies. LipidTOX was used to label lipid droplets. Images were taken under a Zeiss LSM-780 microscope in a 3D Z-stack mode & reconstructed using Imaris 9.2.0. b The other set of cells was subjected to surface biotinylation assay & blotted with indicated antibodies. c, d On day 4 of differentiation, 3T3-L1 cells were infected with lentivirus encoding scrambled shRNA or shRNAs against CD36 or CAV1. On day 5, cells were selected with 5 μg/ml puromycin. On day 8, cells were pretreated as in (a) & treated with oleate (100 μM) for 4 h, followed by immunostaining with anti-CD36 & anti-CAV1 antibodies (c), or surface biotinylation assay (d). e, f 3T3-L1 adipocytes were pretreated as in (a) & treated with BSA-conjugated FAs with different chain lengths or saturation (100 μM) for 4 h. Cells were subjected to immunostaining with anti-CD36 antibody (e), or surface biotinylation assay (f). After oleate treatment for 4 h, 3T3-L1 adipocytes were switched to serum-free medium for indicated time & harvested for immunostaining (g) & surface biotinylation (h). The scale bars were as indicated in each figure. These experiments were repeated at least three times. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-31020241612242.jpg)
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - FAs trigger internalization of CD36.a, b On day 8 of differentiation, 3T3-L1 adipocytes were pretreated with serum-free medium for 4 h, & then treated with BSA-conjugated oleate (100 μM) for indicated time. a One set of cells was subjected to immunostaining with anti-CD36 & anti-CAV1 antibodies. LipidTOX was used to label lipid droplets. Images were taken under a Zeiss LSM-780 microscope in a 3D Z-stack mode & reconstructed using Imaris 9.2.0. b The other set of cells was subjected to surface biotinylation assay & blotted with indicated antibodies. c, d On day 4 of differentiation, 3T3-L1 cells were infected with lentivirus encoding scrambled shRNA or shRNAs against CD36 or CAV1. On day 5, cells were selected with 5 μg/ml puromycin. On day 8, cells were pretreated as in (a) & treated with oleate (100 μM) for 4 h, followed by immunostaining with anti-CD36 & anti-CAV1 antibodies (c), or surface biotinylation assay (d). e, f 3T3-L1 adipocytes were pretreated as in (a) & treated with BSA-conjugated FAs with different chain lengths or saturation (100 μM) for 4 h. Cells were subjected to immunostaining with anti-CD36 antibody (e), or surface biotinylation assay (f). After oleate treatment for 4 h, 3T3-L1 adipocytes were switched to serum-free medium for indicated time & harvested for immunostaining (g) & surface biotinylation (h). The scale bars were as indicated in each figure. These experiments were repeated at least three times. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-310202416114740.jpg)
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - FAs trigger internalization of CD36.a, b On day 8 of differentiation, 3T3-L1 adipocytes were pretreated with serum-free medium for 4 h, & then treated with BSA-conjugated oleate (100 μM) for indicated time. a One set of cells was subjected to immunostaining with anti-CD36 & anti-CAV1 antibodies. LipidTOX was used to label lipid droplets. Images were taken under a Zeiss LSM-780 microscope in a 3D Z-stack mode & reconstructed using Imaris 9.2.0. b The other set of cells was subjected to surface biotinylation assay & blotted with indicated antibodies. c, d On day 4 of differentiation, 3T3-L1 cells were infected with lentivirus encoding scrambled shRNA or shRNAs against CD36 or CAV1. On day 5, cells were selected with 5 μg/ml puromycin. On day 8, cells were pretreated as in (a) & treated with oleate (100 μM) for 4 h, followed by immunostaining with anti-CD36 & anti-CAV1 antibodies (c), or surface biotinylation assay (d). e, f 3T3-L1 adipocytes were pretreated as in (a) & treated with BSA-conjugated FAs with different chain lengths or saturation (100 μM) for 4 h. Cells were subjected to immunostaining with anti-CD36 antibody (e), or surface biotinylation assay (f). After oleate treatment for 4 h, 3T3-L1 adipocytes were switched to serum-free medium for indicated time & harvested for immunostaining (g) & surface biotinylation (h). The scale bars were as indicated in each figure. These experiments were repeated at least three times. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-310202416114741.jpg)
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - CD36-mediated caveolar endocytosis transports FAs into cells.a–c 3T3-L1 adipocytes were pretreated with serum-free medium for 4 h, & then treated with BSA-conjugated PacFA (50 μM) for 20 min, followed by UV crosslinking on ice for 30 min. a, b Cells were subjected to click chemistry using an N3-Alexa Fluro 488, & immunostained with anti-CD36 antibody. Colocalization of PacFA & CD36 was quantified from 24 cells over three independent experiments & plotted in (b). The value represents mean ± SEM. c Cells were lysed & subjected to click chemistry assay using N3-biotin. PacFA-labeled proteins were captured with streptavidin beads & subjected to western blot using anti-CD36 & anti-FABP4 antibodies. d WT, Cav1−/−, Cd36−/−, & Cav1−/−;Cd36−/− SVFs were isolated & differentiated into adipocytes & treated with 100 μM 3H-oleate (specific activity, 2268 dpm/nmol) for 1 h. Lipid fractions were extracted from the cells & subjected to scintillation counting. The radioactive counting was normalized to protein content. Each value represents mean ± SEM obtained from three samples. Two-sided Student’s t test was performed between WT & each of the knockout cells. These experiments were repeated twice. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-310202416113520.jpg)
![Immunocytochemistry/Immunofluorescence: CD27/TNFRSF7 Antibody (LPFS2/1611) [NBP2-53196] - Immunofluorescence staining of Ramos cells using CD27/TNFRSF7 Antibody (LPFS2/1611) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Red Dot.](http://images.novusbio.com/fullsize/CD27-TNFRSF7-Antibody-LPFS2-1611-Immunocytochemistry-Immunofluorescence-NBP2-53196-img0003.jpg "Immunocytochemistry/Immunofluorescence: CD27/TNFRSF7 Antibody (LPFS2/1611) [NBP2-53196] - Immunofluorescence staining of Ramos cells using CD27/TNFRSF7 Antibody (LPFS2/1611) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Red Dot.")
![Immunohistochemistry-Paraffin: CD27/TNFRSF7 Antibody (LPFS2/1611) [NBP2-53196] - Formalin-fixed, paraffin-embedded human Colon stained with CD27/TNFRSF7 Antibody (LPFS2/1611).](http://images.novusbio.com/fullsize/CD27-TNFRSF7-Antibody-LPFS2-1611-Immunohistochemistry-Paraffin-NBP2-53196-img0006.jpg)
![Flow Cytometry: CD27/TNFRSF7 Antibody (LPFS2/1611) [NBP2-53196] - Flow Cytometric Analysis of Ramos cells using CD27/TNFRSF7 Mouse Monoclonal Antibody (LPFS2/1611) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CD27-TNFRSF7-Antibody-LPFS2-1611-Flow-Cytometry-NBP2-53196-img0008.jpg)
![Immunohistochemistry-Paraffin: CD27/TNFRSF7 Antibody (LPFS2/1611) [NBP2-53196] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD27 Monoclonal Antibody (LPFS2/1611).](http://images.novusbio.com/fullsize/CD27-TNFRSF7-Antibody-LPFS2-1611-Immunohistochemistry-Paraffin-NBP2-53196-img0001.jpg)
![Immunohistochemistry-Paraffin: CD27/TNFRSF7 Antibody (LPFS2/1611) [NBP2-53196] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD27/TNFRSF7 Antibody (LPFS2/1611).](http://images.novusbio.com/fullsize/CD27-TNFRSF7-Antibody-LPFS2-1611-Immunohistochemistry-Paraffin-NBP2-53196-img0004.jpg)
![Immunohistochemistry-Paraffin: CD27/TNFRSF7 Antibody (LPFS2/1611) [NBP2-53196] - Formalin-fixed, paraffin-embedded human Spleen stained with CD27/TNFRSF7 Antibody (LPFS2/1611).](http://images.novusbio.com/fullsize/CD27-TNFRSF7-Antibody-LPFS2-1611-Immunohistochemistry-Paraffin-NBP2-53196-img0005.jpg)
![Protein Array: CD27/TNFRSF7 Antibody (LPFS2/1611) [NBP2-53196] - Analysis of Protein Array containing more than 19,000 full-length human proteins using CD27/TNFRSF7 Antibody (LPFS2/1611) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD?s) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD?s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/CD27-TNFRSF7-Antibody-LPFS2-1611-Protein-Array-NBP2-53196-img0007.jpg)
![Flow Cytometry: F4/80 Antibody (BM8) [NBP1-60140] - Splenocytes isolated from a IL1R-/- mouse (IL1b receptor KO) were stained with and CD45-e450 (1:100 in FACS buffer) for 30 minutes. Cells were then washed and analyzed by flow cytometry. Left panel shows the isotype control. This image was submitted via a customer review. Image from the PE version of this antibody.](http://images.novusbio.com/fullsize/F4-80-Antibody-BM8-Flow-Cytometry-NBP1-60140-img0006.jpg "Flow Cytometry: F4/80 Antibody (BM8) [NBP1-60140] - Splenocytes isolated from a IL1R-/- mouse (IL1b receptor KO) were stained with and CD45-e450 (1:100 in FACS buffer) for 30 minutes. Cells were then washed and analyzed by flow cytometry. Left panel shows the isotype control. This image was submitted via a customer review. Image from the PE version of this antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Frozen: F4/80 Antibody (BM8) [NBP1-60140] - Red: F4/80, Green: CD31/PECAM-1, Blue: DAPI+ nuclei. Frozen mouse lung tissues of WT B6 and IL1R--/- (knockout) mice were blocked with 1% BSA in PBS and were double stained with PE-conjugated F4/80 and Alex647-conjugated CD31/PECAM-1 antibody (MEC 7.46) for 2 hours at the room temperature. This image was submitted via a customer review. Image from the PE version of this antibody.](http://images.novusbio.com/fullsize/F4-80-Antibody-BM8-Immunohistochemistry-Frozen-NBP1-60140-img0007.jpg)
![Immunohistochemistry: F4/80 Antibody (BM8) [NBP1-60140] - Expression on macrophages in mouse liver. Staining of frozen tissue section with antibody BM8.](http://images.novusbio.com/fullsize/F4-80-Antibody-BM8-Immunohistochemistry-NBP1-60140-img0003.jpg)
![Flow Cytometry: F4/80 Antibody (BM8) [NBP1-60140] - Analysis using the FITC conjugate of NBP1-60140. Thioglycolate-elicited Balb/c mouse peritoneal macrophages stained with BM8 FITC.](http://images.novusbio.com/fullsize/F4-80-Antibody-BM8-Flow-Cytometry-NBP1-60140-img0005.jpg)
![ELISA: Mouse Complement C3a ELISA Kit (Colorimetric) [NBP2-70037] - Samples were spiked with high concentrations of Mouse Complement C3a and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-70037_mouse-complement-c3a-elisa-kit-colorimetric-13220241626433.jpg "ELISA: Mouse Complement C3a ELISA Kit (Colorimetric) [NBP2-70037] - Samples were spiked with high concentrations of Mouse Complement C3a and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Complement C3a ELISA Kit (Colorimetric) [NBP2-70037] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-70037_mouse-complement-c3a-elisa-kit-colorimetric-432024941599.jpg)
![Flow Cytometry: LAG-3 Antibody (11E3) [NBP1-97662] - Analysis of 10^6 MCF-7 cells stained using LAG-3 (human) monoclonal antibody (11E3) at a concentration of 50ug/mL.](http://images.novusbio.com/fullsize/LAG-3-Antibody-11E3-Flow-Cytometry-NBP1-97662-img0001.jpg "Flow Cytometry: LAG-3 Antibody (11E3) [NBP1-97662] - Analysis of 10^6 MCF-7 cells stained using LAG-3 (human) monoclonal antibody (11E3) at a concentration of 50ug/mL.")
![Western Blot: NLRC3 Antibody [NBP2-75593] - Analysis of NLRC3 on human thymus tissue lysates using anti-NLRC3 antibody at 1/500 dilution.](http://images.novusbio.com/fullsize/NLRC3-Antibody-Western-Blot-NBP2-75593-img0009.jpg "Western Blot: NLRC3 Antibody [NBP2-75593] - Analysis of NLRC3 on human thymus tissue lysates using anti-NLRC3 antibody at 1/500 dilution.")
![Immunocytochemistry/Immunofluorescence: NLRC3 Antibody [NBP2-75593] - Staining NLRC3 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NLRC3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-75593-img0004.jpg)
![Immunohistochemistry-Paraffin: NLRC3 Antibody [NBP2-75593] - Analysis of paraffin-embedded mouse colon tissue using anti-NLRC3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/NLRC3-Antibody-Immunohistochemistry-Paraffin-NBP2-75593-img0008.jpg)
![Flow Cytometry: NLRC3 Antibody [NBP2-75593] - Analysis of MCF-7 cells with NLRC3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/NLRC3-Antibody-Flow-Cytometry-NBP2-75593-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: NLRC3 Antibody [NBP2-75593] - Staining NLRC3 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NLRC3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-75593-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: NLRC3 Antibody [NBP2-75593] - Staining NLRC3 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NLRC3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-75593-img0003.jpg)
![Immunohistochemistry-Paraffin: NLRC3 Antibody [NBP2-75593] - Analysis of paraffin-embedded human colon cancer tissue using anti-NLRC3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/NLRC3-Antibody-Immunohistochemistry-Paraffin-NBP2-75593-img0005.jpg)
![Immunohistochemistry-Paraffin: NLRC3 Antibody [NBP2-75593] - Analysis of paraffin-embedded human spleen tissue using anti-NLRC3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/NLRC3-Antibody-Immunohistochemistry-Paraffin-NBP2-75593-img0006.jpg)
![Immunohistochemistry-Paraffin: NLRC3 Antibody [NBP2-75593] - Analysis of paraffin-embedded human tonsil tissue using anti-NLRC3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/NLRC3-Antibody-Immunohistochemistry-Paraffin-NBP2-75593-img0007.jpg)
![Western Blot: CD3 epsilon Antibody (PC3/188A) [NBP2-53386] - Western Blot Analysis of human Jurkat Cell lysate using CD3 epsilon antibody (PC3/188A).](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-PC3-188A-Western-Blot-NBP2-53386-img0007.jpg "Western Blot: CD3 epsilon Antibody (PC3/188A) [NBP2-53386] - Western Blot Analysis of human Jurkat Cell lysate using CD3 epsilon antibody (PC3/188A).")
![Immunohistochemistry-Paraffin: CD3 epsilon Antibody (PC3/188A) [NBP2-53386] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD3 epsilon Monoclonal Antibody (PC3/188A).](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-PC3-188A-Immunohistochemistry-Paraffin-NBP2-53386-img0001.jpg)
![Western Blot: CD3 epsilon Antibody (PC3/188A) [NBP2-53386] - Western Blot Analysis of human Thymus lysate using Purified CD3 epsilon Antibody (PC3/188A).](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-PC3-188A-Western-Blot-NBP2-53386-img0005.jpg)
![Western Blot: CD3 epsilon Antibody (PC3/188A) [NBP2-53386] - Western Blot Analysis of human Jurkat cell lysate using CD3 epsilon Antibody (PC3/188A).](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-PC3-188A-Western-Blot-NBP2-53386-img0006.jpg)
![Flow Cytometry: CD3 epsilon Antibody (PC3/188A) [NBP2-53386] - Analysis with canine whole blood cells. From a verified customer review.](http://images.novusbio.com/fullsize/nbp2-53386_mouse-monoclonal-cd3-epsilon-antibody-pc3-188a-177202495940.jpg)
![Western Blot: TLR13 Antibody [NBP2-24539] - analysis of TLR13 in rat corpus (tissue is from male rat reproductive tract) lysate in the 1) absence and 2) presence of immunizing peptide using TLR13 antibody at 1 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/TLR13-Antibody-Western-Blot-NBP2-24539-img0001.jpg "Western Blot: TLR13 Antibody [NBP2-24539] - analysis of TLR13 in rat corpus (tissue is from male rat reproductive tract) lysate in the 1) absence and 2) presence of immunizing peptide using TLR13 antibody at 1 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Flow Cytometry: TLR13 Antibody [NBP2-24539] - Intracellular analysis of TLR13 in the TLR13 stable cell line this antibody using TLR13 antibody at 1 ug/10^6 cells (green represents vector control, red represents TLR13 antibody).](http://images.novusbio.com/fullsize/TLR13-Antibody-Flow-Cytometry-NBP2-24539-img0002.jpg)
![Western Blot: Dkk-1 Antibody (2A5) [H00022943-M11] - Analysis of DKK1 expression in U-2 OS (Cat # L022V1).](http://images.novusbio.com/fullsize/Dkk-1-Antibody-2A5-Western-Blot-H00022943-M11-img0012.jpg "Western Blot: Dkk-1 Antibody (2A5) [H00022943-M11] - Analysis of DKK1 expression in U-2 OS (Cat # L022V1).")
![Immunohistochemistry-Paraffin: Dkk-1 Antibody (2A5) [H00022943-M11] - Analysis of monoclonal antibody to DKK1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/Dkk-1-Antibody-2A5-Immunohistochemistry-Paraffin-H00022943-M11-img0009.jpg)
![Immunoprecipitation: Dkk-1 Antibody (2A5) [H00022943-M11] - Analysis of DKK1 transfected lysate using anti-DKK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with DKK1 rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/Dkk-1-Antibody-2A5-Immunoprecipitation-H00022943-M11-img0010.jpg)
![ELISA: Dkk-1 Antibody (2A5) [H00022943-M11] - Detection limit for recombinant GST tagged DKK1 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Dkk-1-Antibody-2A5-ELISA-H00022943-M11-img0013.jpg)
![Western Blot: CXCR4 Antibody [NBP1-76480] - Independent Antibody Validation (IAV) via Protein Expression Profile Loading: 15 ug of lysates per lane. Antibodies: NBP1-77067 (1 ug/mL), NBP1-76480 (1 ug/mL), and beta-actin (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-76480-img0009.jpg "<b>Independent Antibodies Validation. </b>Western Blot: CXCR4 Antibody [NBP1-76480] - Independent Antibody Validation (IAV) via Protein Expression Profile Loading: 15 ug of lysates per lane. Antibodies: NBP1-77067 (1 ug/mL), NBP1-76480 (1 ug/mL), and beta-actin (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST.")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/ Immunofluorescence: CXCR4 Antibody - BSA Free [NBP1-76480] - Figure 5 Immunofluorescence Validation of CXCR4 Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling CXCR4 with at 4 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing both membrane and cytoplasmic staining on HeLa cell line.](http://images.novusbio.com/fullsize/nbp1-76480_rabbit-polyclonal-cxcr4-antibody-2711202419562119.jpg)
![Immunohistochemistry-Paraffin: CXCR4 Antibody [NBP1-76480] - Staining of CXCR4 in mouse spleen tissue with CXCR4 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Immunohistochemistry-Paraffin-NBP1-76480-img0006.jpg)
![Western Blot: CXCR4 Antibody [NBP1-76480] - HeLa total cell lysate.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-76480-img0002.jpg)
![Western Blot: CXCR4 Antibody [NBP1-76480] - Animal Species ReactivityLoading: Lysates/proteins at 20 ug per lane.Antibodies: NBP1-77067 (2 ug/mL) or NBP1-76480 (2 ug/mL). 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-76480-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: CXCR4 Antibody [NBP1-76480] - Validation with CXCR4 siRNA Knockdown. HeLa cells were transfected with control siRNAs (lane 1) or CXCR4 siRNAs (lane 2) Loading: 10 ug of HeLa whole cell lysates per lane. Antibodies: NBP1-76480 (2 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Knockdown-Validated-NBP1-76480-img0008.jpg)