| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-75978SS | |||
| BSA Free | NBP2-75978 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-75977SS | |||
| Azide and BSA Free | NBP2-80764 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-41012-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB120-10505-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-74679SS | |||
![Western Blot: CREB [p Ser133] Antibody [NB300-273] - Rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~45 kDa CREB phosphorylated at Ser133 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase lambda-Ptase, 800 units/1mg protein for 30 min).](http://images.novusbio.com/fullsize/CREB-[p-Ser133]-Antibody-Western-Blot-NB300-273-img0007.jpg "Western Blot: CREB [p Ser133] Antibody [NB300-273] - Rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~45 kDa CREB phosphorylated at Ser133 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase lambda-Ptase, 800 units/1mg protein for 30 min).")
![Immunohistochemistry-Frozen: CREB [p Ser133] Antibody [NB300-273] - IHC Analysis of pCREB S133 (red) in frozen sections from mouse piriform cortex. Cell nuclei are visualized with DAPI DNA stain (blue). NB300-273 was used at a dilution of 1:1000 and incubated for 48 hours at 4C. No antigen retrieval step was performed.](http://images.novusbio.com/fullsize/CREB-[p-Ser133]-Antibody-Immunohistochemistry-Frozen-NB300-273-img0006.jpg)
![Western Blot: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of Iba1 on THP-1 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Western-Blot-NBP2-75397-img0008.jpg "Western Blot: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of Iba1 on THP-1 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Staining AIF-1/Iba1 in SH-SY5Y cells using (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100, PBS.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunocytochemistry-Immunofluorescence-NBP2-75397-img0002.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Immunofluorescence analysis of paraffin-embedded rat brain tissue labeling Iba1 with Rabbit anti-AIF-1/Iba1 antibody washed with PBS, and then probed with the primary antibody (green) at 1/100 dilution overnight at 4 , washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0015.jpg)
![Flow Cytometry: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of THP-1 cells withAIF-1:Iba1 Antibody (JM36-62) at 1:100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Flow-Cytometry-NBP2-75397-img0001.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded human lung cancer tissue using AIF-1/Iba1 Antibody (JM36-62). Counterstained with hematoxylin.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0003.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded human spleen tissue using AIF-1/Iba1 Antibody (JM36-62). Counterstained with hematoxylin.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0004.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded mouse brain tissue using AIF-1/Iba1 Antibody (JM36-62). Counterstained with hematoxylin.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0005.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded mouse spleen tissue using AIF-1/Iba1 Antibody (JM36-62). Counterstained with hematoxylin.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0006.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded rat lung tissue using AIF-1/Iba1 Antibody (JM36-62). Counterstained with hematoxylin.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0007.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Immunofluorescence staining of paraffin- embedded human spleen tissue using anti-AIF-1/Iba1rabbit polyclonal antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.(sodium citrate buffer (pH6) for 20 mins.) The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with at 1/50 dilution for 10 hours at 4C and detected using Alexa Fluor(R) 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0009.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Immunofluorescence staining of paraffin- embedded mouse brain tissue using anti-AIF-1/Iba1 antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with at 1/50 dilution for 10 hours at 4 and detected using Alexa Fluor(R) 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0010.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-AIF-1/Iba1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0011.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded human kidney tissue with Rabbit anti-AIF-1/Iba1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0012.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded human spleen tissue with Rabbit anti-AIF-1/Iba1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0013.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Iba1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0014.jpg)
![Western Blot: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Western-Blot-NBP2-80765-img0001.jpg "Western Blot: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Immunofluorescent staining of HIF-1 alpha using catalog number NBP2-75977 (clone 2443B). Staining was observed in Hela cells treated with DFO but not in untreated Hela cells. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80765-img0002.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - HIF-1 alpha (clone 2443C) was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-Human HIF-1 alpha Monoclonal Antibody (Catalog # NBP2-75978) at 1.0 ug/mL for 1 hour at room temperature followed by incubation with the](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80765-img0003.jpg)
![Simple Western: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Lane view shows a specific band for HIF-1 alpha in HepG2 +/- DFO cell lysate using 20 ug/mL. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 116 KDa. Image from an internal validation. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Simple-Western-NBP2-80765-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HIF-1 alpha knockout HeLa cell line (KO) untreated (-) or treated (+) with 1 mM DFO for overnight. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human HIF-1 alpha Monoclonal Antibody (Catalog # NBP2-75978) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HIF-1 alpha at approximately 120 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Knockout-Validated-NBP2-80765-img0004.jpg)
![Western Blot: P2X7/P2RX7 Antibody [NBP1-20180] - WB on brain lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:5000 incubated at 4C overnight.](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Western-Blot-NBP1-20180-img0006.jpg "Western Blot: P2X7/P2RX7 Antibody [NBP1-20180] - WB on brain lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:5000 incubated at 4C overnight.")
![Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat DRG. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen.Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Immunohistochemistry-Paraffin-NBP1-20180-img0022.jpg)
![Western Blot: P2X7/P2RX7 Antibody [NBP1-20180] - Rat brain lysate using Rabbit antibody to P2RX7 at 1:500 dilution. Incubated 30 min at RT with shake. Blocking: 0.5% LFDM in 1x PBS containing 0.1% Tween-20](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Western-Blot-NBP1-20180-img0005.jpg)
![Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat cerebellum. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Immunohistochemistry-Paraffin-NBP1-20180-img0010.jpg)
![Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat cerebellum. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Immunohistochemistry-Paraffin-NBP1-20180-img0011.jpg)
![Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat DRG. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Immunohistochemistry-Paraffin-NBP1-20180-img0012.jpg)
![Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat DRG. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Immunohistochemistry-Paraffin-NBP1-20180-img0013.jpg)
![Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat cerebellum. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Immunohistochemistry-Paraffin-NBP1-20180-img0014.jpg)
![Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat cerebellum. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Immunohistochemistry-Paraffin-NBP1-20180-img0015.jpg)
![Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat cerebellum. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Immunohistochemistry-Paraffin-NBP1-20180-img0016.jpg)
![Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat cerebellum. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Immunohistochemistry-Paraffin-NBP1-20180-img0017.jpg)
![Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Immunohistochemistry-Paraffin-NBP1-20180-img0018.jpg)
![Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Immunohistochemistry-Paraffin-NBP1-20180-img0019.jpg)
![Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Immunohistochemistry-Paraffin-NBP1-20180-img0020.jpg)
![Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat cerebellum. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Immunohistochemistry-Paraffin-NBP1-20180-img0021.jpg)
![Western Blot: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membranes were blotted with AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Western-Blot-NBP3-13648-img0010.jpg "<b>Independent Antibodies Validation. </b>Western Blot: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membranes were blotted with AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - Rat brain. AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Immunohistochemistry-Paraffin-NBP3-13648-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Frozen: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - AIF-1/Iba1 antibody [HL22] detects AIF-1/Iba1 protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse spinal cord. Green: AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:200. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Immunohistochemistry-Frozen-NBP3-13648-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - Mouse hippocampus. AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Immunohistochemistry-Paraffin-NBP3-13648-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - Rat cerebellum. AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Immunohistochemistry-Paraffin-NBP3-13648-img0003.jpg)
![Immunohistochemistry-Frozen: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - AIF-1/Iba1 antibody [HL22] detects AIF-1/Iba1 protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse brain. Green: AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:100. Red: AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22-RT] diluted at 1:100. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Immunohistochemistry-Frozen-NBP3-13648-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Iba1 stained by Iba1 antibody [HL22] diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp3-13648_rabbit-aif-1-iba1-mab-hl22-immunocytochemistry-immunofluorescence-244202411033..jpg)
![Iba1 antibody [HL22] detects Iba1 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.Iba1 stained by Iba1 antibody [HL22] (NBP3-13648) conjugated with DyLight488 diluted at 1:200.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/nbp3-13648_rabbit-aif-1-iba1-mab-hl22-immunohistochemistry-paraffin-261120251850420.jpg)
![Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody [HL22] (NBP3-13648) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-13648_rabbit-aif-1-iba1-mab-hl22-western-blot-2611202518454020.jpg)
![Western Blot: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Western-Blot-NBP2-75977-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HIF-1 alpha knockout HeLa cell line (KO) untreated (-) or treated (+) with 1 mM DFO for overnight. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human HIF-1 alpha Monoclonal Antibody (NBP2-75977) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HIF-1 alpha at an approximatel molecular weight of 120 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control.This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Knockdown-Validated-NBP2-75977-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Immunofluorescent staining of HIF-1 alpha using catalog number NBP2-75977 (clone 2443B). Staining was observed in Hela cells treated with DFO but not in untreated Hela cells.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Immunocytochemistry-Immunofluorescence-NBP2-75977-img0001.jpg)
![Simple Western: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Lane view shows a specific band for HIF-1 alpha in HepG2 +/- DFO cell lysate using 20ug/ml. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 116 KDa. Image from an internal validation.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Simple-Western-NBP2-75977-img0004.jpg)
![Simple Western: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Simple Western lane view shows lysates of MCF +/- DMOG, BioSpherix MSCs in hypoxic conditions, and BioSpherix MSCs in normoxic conditions loaded at 0.5 mg/ml. A band was detected for HIF-1 alpha at approximately 116 kDa (as indicated) using NBP2-75977 (1:100 dilution) followed by Anti-Rabbit Secondary Antibody (042-206, ProteinSimple). This experiment was conducted under standard assay conditions, and using the 12-230 kDa separation module (SW-W004). Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody. Image from an internal validation.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Simple-Western-NBP2-75977-img0005.jpg)
![Flow Cytometry: CYBB/NOX2 Antibody (NL7) [NBP1-41012] - Using the Alexa Fluor 647 direct conjugate An intracellular stain was performed on Daudi cells with CYBB/NOX2 (NL7) antibody NBP1-41012AF647 (blue) and a matched isotype control NBP1-97005AF647 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/CYBB-NOX2-Antibody-NL7-Flow-Cytometry-NBP1-41012-img0004.jpg "Flow Cytometry: CYBB/NOX2 Antibody (NL7) [NBP1-41012] - Using the Alexa Fluor 647 direct conjugate An intracellular stain was performed on Daudi cells with CYBB/NOX2 (NL7) antibody NBP1-41012AF647 (blue) and a matched isotype control NBP1-97005AF647 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.")
![Flow Cytometry: CYBB/NOX2 Antibody (NL7) [NBP1-41012] - Staining of human peripheral blood granulocytes with Mouse anti Human Cytochrome b-245 Heavy Chain. Permeabilised with Leucoperm](http://images.novusbio.com/fullsize/CYBB-NOX2-Antibody-NL7-Flow-Cytometry-NBP1-41012-img0001.jpg)
![Flow Cytometry: CYBB/NOX2 Antibody (NL7) [NBP1-41012] - Analysis using the PE conjugate of NBP1-41012. Staining of CYBB in human peripheral blood using anti-CYBB antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/CYBB-NOX2-Antibody-NL7-Flow-Cytometry-NBP1-41012-img0003.jpg)
![Flow (Intracellular): CYBB/NOX2 Antibody (NL7) [NBP1-41012] - An intracellular stain was performed on THP-1 cells with CYBB/NOX2 (NL7) antibody NBP1-41012AF647 (blue) and a matched isotype control(orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/CYBB-NOX2-Antibody-NL7-Flow-Intracellular-NBP1-41012-img0005.jpg)
![Western Blot: CREB Antibody [NBP2-22516] - Analysis of GH4 cell extract.](http://images.novusbio.com/fullsize/CREB-Antibody-Western-Blot-NBP2-22516-img0001.jpg "Western Blot: CREB Antibody [NBP2-22516] - Analysis of GH4 cell extract.")
![Immunocytochemistry/Immunofluorescence: CREB Antibody [NBP2-22516] - Analysis of CREB (green) showing staining in the nucleus of Neuro-2a cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/CREB-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-22516-img0004.jpg)
![Immunohistochemistry-Paraffin: CREB Antibody [NBP2-22516] - Analysis showing staining in the nucleus of human glioma (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/CREB-Antibody-Immunohistochemistry-Paraffin-NBP2-22516-img0007.jpg)
![Chromatin Immunoprecipitation: CREB Antibody [NBP2-22516] - Analysis performed using cross-linked chromatin from colon carcinoma cells treated with serum. IP was performed using a multiplex microplate Matrix ChIP assay of a CREB polyclonal antibody. Chromatin aliquots from cells were used per ChIP pull-down. Quantitative PCR data one in quadruplicate using 1ul of eluted DNA in 2ul SYBR real-time PCR reactions containing primers to amplify -15kb upstream of Egr1 or exon-1 or exon-2 of Egr1.Quantitation of immunoprecipitated chromatin is presented as signal relative to the total amount of input chromatin. Results represent the mean +/- SEM. A schematic representation of the Egr-1 locus is shown; xes represent exons (black boxes = translated regions, white boxes = untranslated regions), the zigzag line represents an intron, and the straight line represents upstream sequence. Regions amplified by Egr-1 primers are represented by black bars. Data courtesy of the Innovators Program.](http://images.novusbio.com/fullsize/CREB-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP2-22516-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CREB Antibody [NBP2-22516] - Analysis of CREB (green) showing staining in the nucleus of SK-N-MC cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/CREB-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-22516-img0003.jpg)
![Immunohistochemistry-Paraffin: CREB Antibody [NBP2-22516] - Analysis showing staining in the nucleus of mouse brain tissue (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/CREB-Antibody-Immunohistochemistry-Paraffin-NBP2-22516-img0005.jpg)
![Immunohistochemistry-Paraffin: CREB Antibody [NBP2-22516] - Analysis showing staining in the nucleus of human lung adenocarcinoma (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/CREB-Antibody-Immunohistochemistry-Paraffin-NBP2-22516-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: BDNF Antibody (35928.11) [NB120-10505] - Human thyroid tissue was incubated with anti-BDNF antibody at a dilution of 1:100 overnight at 4C and detected with Alexa Fluor 594 Donkey anti-mouse 1:500. Nuclei stained with DAPI. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/BDNF-Antibody-35928-11-Immunocytochemistry-Immunofluorescence-NB120-10505-img0001.jpg "Immunocytochemistry/Immunofluorescence: BDNF Antibody (35928.11) [NB120-10505] - Human thyroid tissue was incubated with anti-BDNF antibody at a dilution of 1:100 overnight at 4C and detected with Alexa Fluor 594 Donkey anti-mouse 1:500. Nuclei stained with DAPI. ICC/IF image submitted by a verified customer review.")
![Western Blot: HIF-1 alpha [Hydroxy Pro564] Antibody [NB110-74679] - Detection (left panel) of HIF-1 alpha [Hydroxy Pro564] in nuclear extracts of A549 cells treated with MG132 (a proteasome inhibitor). Hydroxyproline is not recognized on HIF-1alpha when cells are first treated with DFO, a prolyl hydroxylase inhibitor that prevents HIF hydroxylation. Control staining is shown (right panel) using conventional anti-HIF-1alpha. The asterisk marks a band approximately 110 kDa molecular weight in size corresponding to HIF-1 alpha. The primary antibody was used at a 1:1000 dilution in 2% BLOTTO.](http://images.novusbio.com/fullsize/HIF-1-alpha-[Hydroxy-Pro564]-Antibody-Western-Blot-NB110-74679-img0004.jpg "Western Blot: HIF-1 alpha [Hydroxy Pro564] Antibody [NB110-74679] - Detection (left panel) of HIF-1 alpha [Hydroxy Pro564] in nuclear extracts of A549 cells treated with MG132 (a proteasome inhibitor). Hydroxyproline is not recognized on HIF-1alpha when cells are first treated with DFO, a prolyl hydroxylase inhibitor that prevents HIF hydroxylation. Control staining is shown (right panel) using conventional anti-HIF-1alpha. The asterisk marks a band approximately 110 kDa molecular weight in size corresponding to HIF-1 alpha. The primary antibody was used at a 1:1000 dilution in 2% BLOTTO.")
![Western Blot: P2X7/P2RX7 Antibody [NBP1-37775] - Staining of Human Brain (Frontal Cortex) lysate (35 ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Western-Blot-NBP1-37775-img0005.jpg "Western Blot: P2X7/P2RX7 Antibody [NBP1-37775] - Staining of Human Brain (Frontal Cortex) lysate (35 ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-37775] - Staining of paraffin embedded Human Kidney. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Immunohistochemistry-Paraffin-NBP1-37775-img0007.jpg)
![Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-37775] - Staining of paraffin embedded Human Brain Cortex. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Immunohistochemistry-Paraffin-NBP1-37775-img0006.jpg)
![Western Blot: BDNF Antibody (SJ12-09) [NBP2-67410] - Analysis of BDNF on different lysates using anti-BDNF antibody at 1/1,000 dilution. Positive control: Lane 1: SHG-44 Lane 2: A172 Lane 3: Mouse brain](http://images.novusbio.com/fullsize/BDNF-Antibody-SJ12-09-Western-Blot-NBP2-67410-img0005.jpg "Western Blot: BDNF Antibody (SJ12-09) [NBP2-67410] - Analysis of BDNF on different lysates using anti-BDNF antibody at 1/1,000 dilution. Positive control: Lane 1: SHG-44 Lane 2: A172 Lane 3: Mouse brain")
![Immunocytochemistry/Immunofluorescence: BDNF Antibody (SJ12-09) [NBP2-67410] - ICC staining of BDNF in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary ant](http://images.novusbio.com/fullsize/BDNF-Antibody-SJ12-09-Immunocytochemistry-Immunofluorescence-NBP2-67410-img0006.jpg)
![Immunohistochemistry-Paraffin: BDNF Antibody (SJ12-09) [NBP2-67410] - Analysis of paraffin-embedded mouse testis tissue using anti-BDNF antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/BDNF-Antibody-SJ12-09-Immunohistochemistry-Paraffin-NBP2-67410-img0004.jpg)
![Immunohistochemistry-Paraffin: BDNF Antibody (SJ12-09) [NBP2-67410] - Analysis of paraffin-embedded human lung tissue using anti-BDNF antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/BDNF-Antibody-SJ12-09-Immunohistochemistry-Paraffin-NBP2-67410-img0001.jpg)
![Immunohistochemistry-Paraffin: BDNF Antibody (SJ12-09) [NBP2-67410] - Analysis of paraffin-embedded mouse brain tissue using anti-BDNF antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/BDNF-Antibody-SJ12-09-Immunohistochemistry-Paraffin-NBP2-67410-img0002.jpg)
![Immunohistochemistry-Paraffin: BDNF Antibody (SJ12-09) [NBP2-67410] - Analysis of paraffin-embedded mouse heart tissue using anti-BDNF antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/BDNF-Antibody-SJ12-09-Immunohistochemistry-Paraffin-NBP2-67410-img0003.jpg)