| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-77666SS | |||
| Azide and BSA Free | NBP2-80718 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-18816SS | |||
| Azide and BSA Free | NBP3-18817 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Allophycocyanin | NB7494APC | |||
| FITC | NB7496 | |||
| HRP | NB7497 | |||
| Janelia Fluor 525 | NB7494JF525 | |||
| Janelia Fluor 669 | NB7494JF669 | |||
| PE | NB7494PE | |||
| PerCP | NB7494PCP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP2-22358 | |||
| DyLight 350 | NB7237UV | |||
| DyLight 405 | NB7237V | |||
| DyLight 488 | NB7237G | |||
| DyLight 550 | NB7237R | |||
| DyLight 594 | NB7237DL594 | |||
| DyLight 650 | NB7237C | |||
| DyLight 680 | NB7237FR | |||
| DyLight 755 | NB7237IR | |||
| FITC | NB7237F | |||
| HRP | NB7238 | |||
| Janelia Fluor 525 | NB7237JF525 | |||
| Janelia Fluor 669 | NB7237JF669 | |||
| PE | NB7237PE | |||
| PerCP | NB7237PCP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Allophycocyanin | NB7463APC | |||
| Biotin | NB7463B | |||
| DyLight 350 | NB7463UV | |||
| DyLight 405 | NB7463V | |||
| DyLight 488 | NB7463G | |||
| DyLight 550 | NB7463R | |||
| DyLight 594 | NB7463DL594 | |||
| DyLight 650 | NB7463C | |||
| DyLight 680 | NB7463FR | |||
| DyLight 755 | NB7463IR | |||
| FITC | NB7465 | |||
| HRP | NB7466 | |||
| Janelia Fluor 525 | NB7463JF525 | |||
| Janelia Fluor 669 | NB7463JF669 | |||
| PE | NB7463PE | |||
| PerCP | NB7463PCP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76538-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-27405 | |||
| BSA Free | NBP2-27404SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87781-25ul | |||
![Western Blot: Endoglin/CD105 Antibody (MJ7/18) [NB100-77666] - Total protein from mouse placenta was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocking in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-CD105 (NB100-77666) in 1% milk/TBST and detected with an anti-rat HRP conjugated secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MJ7-18-Western-Blot-NB100-77666-img0001.jpg "Western Blot: Endoglin/CD105 Antibody (MJ7/18) [NB100-77666] - Total protein from mouse placenta was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocking in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-CD105 (NB100-77666) in 1% milk/TBST and detected with an anti-rat HRP conjugated secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (MJ7/18) [NB100-77666] - Using the DyLight 550 direct conjugate Staining of CD105 (endoglin) in 4T1 tumors after radioimmunotherapy shows CD105 expression (red), blood vessels (green), and nuclei (blue).](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MJ7-18-Immunocytochemistry-Immunofluorescence-NB100-77666-img0004.jpg)
![Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody (MJ7/18) [NB100-77666] - Analysis of a FFPE mouse ductus deference using a 1:50 dilution of CD105 antibody (clone MJ7/18) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) was performed using Aperio AT2 (Leica Biosystems). Staining was observed in the pseudostratified columnar epithelial cells. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MJ7-18-Immunohistochemistry-Paraffin-NB100-77666-img0005.jpg)
![Flow Cytometry: Endoglin/CD105 Antibody (MJ7/18) [NB100-77666] - Analysis of Biotin conjugate of NB100-77666. Surface staining of bEND.3 cell line with 0.25 ug of Anti-Mouse CD105 (Endoglin) Biotin followed by Streptavidin PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MJ7-18-Flow-Cytometry-NB100-77666-img0002.jpg)
![Flow (Cell Surface): Endoglin/CD105 Antibody (MJ7/18) [NB100-77666] - A surface stain was performed on MS-1 Cells with Endoglin/CD105 (MJ7/18) antibody NB100-77666 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by rat F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0113, R&D Systems).](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MJ7-18-Flow-Cell-Surface-NB100-77666-img0003.jpg)
![Western Blot: STING/TMEM173 Antibody (2922D) [NBP3-18816] - Western blot shows cell lysates K562, THP-1, C2C12, and C6. Membrane was probed with 1 ug/mL of (Catalog # NBP3-18816) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for STING/TMEM173 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. Internally validated Western blot.](http://images.novusbio.com/fullsize/STING-TMEM173-Antibody-2922D-Western-Blot-NBP3-18816-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: STING/TMEM173 Antibody (2292D) [NBP3-18816] - STING/TMEM173 was detected in immersion fixed U937 human myeloid leukaemia cell line but is not detected in Daudi cell line using Rabbit Anti-Human STING/TMEM173 Monoclonal Antibody (Catalog # NBP3-18816) at 1 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights(TM) 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/STING-TMEM173-Antibody-2292D-Immunocytochemistry-Immunofluorescence-NBP3-18816-img0002.jpg)
![Immunohistochemistry: STING/TMEM173 Antibody (2292D) [NBP3-18816] - STING/TMEM173 was detected in immersion fixed paraffin-embedded sections of mouse lung tissue using Rabbit Anti-Human STING/TMEM173 Monoclonal Antibody at 0.5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to pneumocytes and alveolar cells. Staining with VisUCyte HRP Polymer Detection Reagents.](http://images.novusbio.com/fullsize/STING-TMEM173-Antibody-2292D-Immunohistochemistry-NBP3-18816-img0001.jpg)
![Simple Western: STING/TMEM173 Antibody (2922D) [NBP3-18816] - Simple Western Lane view shows K562 and THP-1 whole cell lysate (WCL). A specific band was detected for hSTING/TMEM173 antibody (NBP3-18816) at approximately 44 kDa (as indicated) using 10 ug/mL of hSTING/TMEM173 antibody. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/STING-TMEM173-Antibody-2922D-Simple-Western-NBP3-18816-img0004.jpg)
![Western Blot: beta 2-Microglobulin Antibody [NBP2-15566] - Lane 1: HEK cells. Lane 2: HEK cells with siRNA against beta 2-Microglobulin antibody. Lane 3: HEK cells stimulated for increased beta 2-Microglobulin expression. Image from verified customer review.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-Western-Blot-NBP2-15566-img0015.jpg "Western Blot: beta 2-Microglobulin Antibody [NBP2-15566] - Lane 1: HEK cells. Lane 2: HEK cells with siRNA against beta 2-Microglobulin antibody. Lane 3: HEK cells stimulated for increased beta 2-Microglobulin expression. Image from verified customer review.")
![Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody [NBP2-15566] - Mouse spleen. B2M stained by Beta 2 Microglobulin antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-Immunohistochemistry-Paraffin-NBP2-15566-img0016.jpg)
![Western Blot: beta 2-Microglobulin Antibody [NBP2-15566] - Rat tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with beta 2 Microglobulin antibody diluted at 1:500.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-Western-Blot-NBP2-15566-img0008.jpg)
![Western Blot: beta 2-Microglobulin Antibody [NBP2-15566] - Mouse plasma (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with beta 2 Microglobulin antibody diluted at 1:1500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-Western-Blot-NBP2-15566-img0012.jpg)
![Western Blot: beta 2-Microglobulin Antibody [NBP2-15566] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with beta 2-Microglobulin antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-Western-Blot-NBP2-15566-img0014.jpg)
![Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody [NBP2-15566] - Cal27 xenograft. beta 2 Microglobulin antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-Immunohistochemistry-Paraffin-NBP2-15566-img0005.jpg)
![Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody [NBP2-15566] - Human endometrial cancer. beta 2 Microglobulin antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-Immunohistochemistry-Paraffin-NBP2-15566-img0007.jpg)
![Immunoprecipitation: beta 2-Microglobulin Antibody [NBP2-15566] - HeLa whole cell extract A. 30 ug HeLa whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of B2M protein by 4 ug beta 2 Microglobulin antibody 15 % SDS-PAGE The mmunoprecipitated B2M protein was detected by beta 2 Microglobulin antibody diluted at 1:500.[EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-Immunoprecipitation-NBP2-15566-img0010.jpg)
![Western Blot: beta 2-Microglobulin Antibody [NBP2-15566] - Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with beta 2 Microglobulin antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp2-15566_rabbit-polyclonal-beta-2-microglobulin-antibody-western-blot-16122024101912..png)
![Immunohistochemistry-Frozen: Myeloperoxidase/MPO Antibody (8F4) [NBP1-51148] - Frozen 8 um rat liver tissue section fixed in formalin. MPO antibody at 1:50 (green). Multiplexed with a CD68 antibody (red). DAPI staining in blue. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Myeloperoxidase-MPO-Antibody-8F4-Immunohistochemistry-Frozen-NBP1-51148-img0001.jpg "Immunohistochemistry-Frozen: Myeloperoxidase/MPO Antibody (8F4) [NBP1-51148] - Frozen 8 um rat liver tissue section fixed in formalin. MPO antibody at 1:50 (green). Multiplexed with a CD68 antibody (red). DAPI staining in blue. IHC-Fr image submitted by a verified customer review.")
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Scanning electron microscopy (SEM) of FACS sorted B cells. P. alecto splenocytes were surface-stained for CD11b, MHC-II and Ig antibodies and sorted as CD11b- MHC-II+ sIg+ (B cells), CD11b+ (myeloid cells) and CD11b- MHC-II- sIg- cells (T cells). Sorted B cells were processed for viewing under a scanning electron microscope. Scanned electron micrograph of sorted Pteropus alecto MHC-II+sIg+ B cells. Bar represents 2 um. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fimmu.2019.00489/full), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Goat-anti-Bat-IgG-H+L-Secondary-Antibody-Flow-Cytometry-NB7237-img0002.jpg "Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Scanning electron microscopy (SEM) of FACS sorted B cells. P. alecto splenocytes were surface-stained for CD11b, MHC-II and Ig antibodies and sorted as CD11b- MHC-II+ sIg+ (B cells), CD11b+ (myeloid cells) and CD11b- MHC-II- sIg- cells (T cells). Sorted B cells were processed for viewing under a scanning electron microscope. Scanned electron micrograph of sorted Pteropus alecto MHC-II+sIg+ B cells. Bar represents 2 um. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fimmu.2019.00489/full), licensed under a CC-BY license.")
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Strategy for immunophenotyping of lymphocytes in P. alecto.Bat splenocytes were stained with cross-reactive antibodies and analysed by FACS. Strategy to identify B cells based on CD3- MHCII+ and IgG+ staining. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep37796), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Goat-anti-Bat-IgG-H+L-Secondary-Antibody-Flow-Cytometry-NB7237-img0001.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Bat T cell production of cytokines & cytolytic factors upon mitogenic stimulation.Bat splenocytes (a–c,g–i) or PBMCs (d–f) were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Detection of intracellular TNF (a), IFN-gamma (b), IL-10 (c) was performed at the protein level, & production of IL- 2 (d), granzyme B (e), perforin (f), IL-17a (g), IL-22 (h) & TGF-beta 1 (i) was detected at the mRNA level by Flow-FISH. Dot plots obtained with one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-310202415395915.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Production of cytokines by B & NK cells upon mitogenic stimulation.Bat splenocytes or PBMCs were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Cells were gated on CD3− MHCII+ (B cells) (a,b) or CD3− Tbet+ Eomes+ (NK cells). Production of intracellular TNF (a,c), IL-10 (b), IFN-gamma (d) was done at the protein level, & production of granzyme B & perforin (e) was performed at the mRNA level by Flow-FISH. Dot plots from one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-31020241534359.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Bat T cell production of cytokines & cytolytic factors upon mitogenic stimulation.Bat splenocytes (a–c,g–i) or PBMCs (d–f) were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Detection of intracellular TNF (a), IFN-gamma (b), IL-10 (c) was performed at the protein level, & production of IL- 2 (d), granzyme B (e), perforin (f), IL-17a (g), IL-22 (h) & TGF-beta 1 (i) was detected at the mRNA level by Flow-FISH. Dot plots obtained with one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-310202415175281.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Bat T cell production of cytokines & cytolytic factors upon mitogenic stimulation.Bat splenocytes (a–c,g–i) or PBMCs (d–f) were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Detection of intracellular TNF (a), IFN-gamma (b), IL-10 (c) was performed at the protein level, & production of IL- 2 (d), granzyme B (e), perforin (f), IL-17a (g), IL-22 (h) & TGF-beta 1 (i) was detected at the mRNA level by Flow-FISH. Dot plots obtained with one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-31020241535683.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Intracellular & surface Ig staining of P. alecto splenocytes. Dead cells were excluded using Live/Dead eFluor 506 dye & live cells were gated for singlets using forward scatter height (FSC-H) & area (FSC-A). For all the staining approaches (surface only, intracellular only, or surface + intracellular), a distinct sIg+ CD3+/− population is observed. Greater than 85% of this population is MHCII+. Comparable percentages of these cells were obtained with surface staining only vs. intracellular staining only, thus strongly suggesting that this population is truly a sIg+ B cell population. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30930908), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-310202415175251.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Production of cytokines by B & NK cells upon mitogenic stimulation.Bat splenocytes or PBMCs were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Cells were gated on CD3− MHCII+ (B cells) (a,b) or CD3− Tbet+ Eomes+ (NK cells). Production of intracellular TNF (a,c), IL-10 (b), IFN-gamma (d) was done at the protein level, & production of granzyme B & perforin (e) was performed at the mRNA level by Flow-FISH. Dot plots from one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-310202415345310.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Production of cytokines by B & NK cells upon mitogenic stimulation.Bat splenocytes or PBMCs were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Cells were gated on CD3− MHCII+ (B cells) (a,b) or CD3− Tbet+ Eomes+ (NK cells). Production of intracellular TNF (a,c), IL-10 (b), IFN-gamma (d) was done at the protein level, & production of granzyme B & perforin (e) was performed at the mRNA level by Flow-FISH. Dot plots from one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-310202415284518.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Detection of T cell subsets by combined Flow-FISH & antibody staining.Bat splenocytes were stained with CD3, Tbet, Eomes & Gata3 antibodies followed by in-situ hybridization with probes specific for CD4mRNA (a) & CD8mRNA (b). One data set is shown & is representative of 2 independent experiments performed with 2 different bat spleens. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-31020241534323.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Bat T cell production of cytokines & cytolytic factors upon mitogenic stimulation.Bat splenocytes (a–c,g–i) or PBMCs (d–f) were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Detection of intracellular TNF (a), IFN-gamma (b), IL-10 (c) was performed at the protein level, & production of IL- 2 (d), granzyme B (e), perforin (f), IL-17a (g), IL-22 (h) & TGF-beta 1 (i) was detected at the mRNA level by Flow-FISH. Dot plots obtained with one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-310202415371931.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Bat T cell production of cytokines & cytolytic factors upon mitogenic stimulation.Bat splenocytes (a–c,g–i) or PBMCs (d–f) were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Detection of intracellular TNF (a), IFN-gamma (b), IL-10 (c) was performed at the protein level, & production of IL- 2 (d), granzyme B (e), perforin (f), IL-17a (g), IL-22 (h) & TGF-beta 1 (i) was detected at the mRNA level by Flow-FISH. Dot plots obtained with one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-310202415345349.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Strategy for immunophenotyping of lymphocytes in P. alecto.Bat splenocytes were stained with cross-reactive antibodies & analysed by FACS. (a) Gating strategy based on live cells, singlets & lymphocyte region. (b) CD3+ cell population analysed for the expression of transcription factors Gata3, Tbet & Eomes. (c) Strategy to identify B cells based on CD3− MHCII+ & IgG+ staining. (d) Strategy to identify NK cells based on CD3−, Eomes+ & Tbet+ staining. One set of data obtained from one bat spleen is shown, & is representative of 4 different bat spleens analyzed. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-31020241537191.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Strategy for immunophenotyping of lymphocytes in P. alecto.Bat splenocytes were stained with cross-reactive antibodies & analysed by FACS. (a) Gating strategy based on live cells, singlets & lymphocyte region. (b) CD3+ cell population analysed for the expression of transcription factors Gata3, Tbet & Eomes. (c) Strategy to identify B cells based on CD3− MHCII+ & IgG+ staining. (d) Strategy to identify NK cells based on CD3−, Eomes+ & Tbet+ staining. One set of data obtained from one bat spleen is shown, & is representative of 4 different bat spleens analyzed. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-310202415392567.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Detection of T cell subsets by combined Flow-FISH & antibody staining.Bat splenocytes were stained with CD3, Tbet, Eomes & Gata3 antibodies followed by in-situ hybridization with probes specific for CD4mRNA (a) & CD8mRNA (b). One data set is shown & is representative of 2 independent experiments performed with 2 different bat spleens. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-310202415291918.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Bat T cell production of cytokines & cytolytic factors upon mitogenic stimulation.Bat splenocytes (a–c,g–i) or PBMCs (d–f) were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Detection of intracellular TNF (a), IFN-gamma (b), IL-10 (c) was performed at the protein level, & production of IL- 2 (d), granzyme B (e), perforin (f), IL-17a (g), IL-22 (h) & TGF-beta 1 (i) was detected at the mRNA level by Flow-FISH. Dot plots obtained with one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-310202415291938.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Bat T cell production of cytokines & cytolytic factors upon mitogenic stimulation.Bat splenocytes (a–c,g–i) or PBMCs (d–f) were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Detection of intracellular TNF (a), IFN-gamma (b), IL-10 (c) was performed at the protein level, & production of IL- 2 (d), granzyme B (e), perforin (f), IL-17a (g), IL-22 (h) & TGF-beta 1 (i) was detected at the mRNA level by Flow-FISH. Dot plots obtained with one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-310202415175290.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Bat T cell production of cytokines & cytolytic factors upon mitogenic stimulation.Bat splenocytes (a–c,g–i) or PBMCs (d–f) were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Detection of intracellular TNF (a), IFN-gamma (b), IL-10 (c) was performed at the protein level, & production of IL- 2 (d), granzyme B (e), perforin (f), IL-17a (g), IL-22 (h) & TGF-beta 1 (i) was detected at the mRNA level by Flow-FISH. Dot plots obtained with one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-310202415345340.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Production of cytokines by B & NK cells upon mitogenic stimulation.Bat splenocytes or PBMCs were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Cells were gated on CD3− MHCII+ (B cells) (a,b) or CD3− Tbet+ Eomes+ (NK cells). Production of intracellular TNF (a,c), IL-10 (b), IFN-gamma (d) was done at the protein level, & production of granzyme B & perforin (e) was performed at the mRNA level by Flow-FISH. Dot plots from one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-31020241539458.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Bat T cell production of cytokines & cytolytic factors upon mitogenic stimulation.Bat splenocytes (a–c,g–i) or PBMCs (d–f) were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Detection of intracellular TNF (a), IFN-gamma (b), IL-10 (c) was performed at the protein level, & production of IL- 2 (d), granzyme B (e), perforin (f), IL-17a (g), IL-22 (h) & TGF-beta 1 (i) was detected at the mRNA level by Flow-FISH. Dot plots obtained with one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-310202415371920.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Strategy for immunophenotyping of lymphocytes in P. alecto.Bat splenocytes were stained with cross-reactive antibodies & analysed by FACS. (a) Gating strategy based on live cells, singlets & lymphocyte region. (b) CD3+ cell population analysed for the expression of transcription factors Gata3, Tbet & Eomes. (c) Strategy to identify B cells based on CD3− MHCII+ & IgG+ staining. (d) Strategy to identify NK cells based on CD3−, Eomes+ & Tbet+ staining. One set of data obtained from one bat spleen is shown, & is representative of 4 different bat spleens analyzed. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-310202415291963.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Expression of MHCII molecules by CD3+ T cells.(a) FACS analysis of splenocytes from one bat based on detection of MHCII & CD3 molecules. (b) Individual percentages of MHCII+ T cells in spleen, MLN, PBMCs & BM from 3–4 bats. The mean (horizontal bar) & SEM are shown. (c) Dot plot featuring Tbet & Eomes expression in MHCII+ & MHCII− CD3+ splenocytes from one bat. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-31020241536374.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Expression of MHCII molecules by CD3+ T cells.(a) FACS analysis of splenocytes from one bat based on detection of MHCII & CD3 molecules. (b) Individual percentages of MHCII+ T cells in spleen, MLN, PBMCs & BM from 3–4 bats. The mean (horizontal bar) & SEM are shown. (c) Dot plot featuring Tbet & Eomes expression in MHCII+ & MHCII− CD3+ splenocytes from one bat. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-310202415363745.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Production of cytokines by B & NK cells upon mitogenic stimulation.Bat splenocytes or PBMCs were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Cells were gated on CD3− MHCII+ (B cells) (a,b) or CD3− Tbet+ Eomes+ (NK cells). Production of intracellular TNF (a,c), IL-10 (b), IFN-gamma (d) was done at the protein level, & production of granzyme B & perforin (e) was performed at the mRNA level by Flow-FISH. Dot plots from one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-310202415371966.jpg)
![Immunocytochemistry/Immunofluorescence: Goat anti-Human Kappa Light Chain Secondary Antibody [NB7463] - JJN3 and 8226 Human myeloma cells stained with NB7463. Cells fixed in 4%PFA 10min, wash PBS X 3. Permeabilized 0.5% Triton X-100 5min; wash PBS X 3. Block 10% donkey serum 1hr. 1:10,000 NB7463 O/N at 4C; wash PBS X 3 and 1:2000 donkey F(ab')2 Alexa Fluor Cy3 anti-goat IgG (H+L) 1 hour at room temp; wash PBS X 3. Olympus/BIOVIEW Cy3 filter 300ms exposure. Image from verified customer review.](http://images.novusbio.com/fullsize/Goat-anti-Human-Kappa-Light-Chain-Secondary-Antibody-Immunocytochemistry-Immunofluorescence-NB7463-img0001.jpg "Immunocytochemistry/Immunofluorescence: Goat anti-Human Kappa Light Chain Secondary Antibody [NB7463] - JJN3 and 8226 Human myeloma cells stained with NB7463. Cells fixed in 4%PFA 10min, wash PBS X 3. Permeabilized 0.5% Triton X-100 5min; wash PBS X 3. Block 10% donkey serum 1hr. 1:10,000 NB7463 O/N at 4C; wash PBS X 3 and 1:2000 donkey F(ab')2 Alexa Fluor Cy3 anti-goat IgG (H+L) 1 hour at room temp; wash PBS X 3. Olympus/BIOVIEW Cy3 filter 300ms exposure. Image from verified customer review.")
![Western Blot: RHAMM/CD168 Antibody - BSA Free [NBP1-76538] - Loading: 10 μg of lysate Antibodies: RHAMM/CD168 , 1 ug/mL and beta-actin, 1ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/antibody/nbp1-76538_rabbit-polyclonal-rhamm-cd168-antibody-western-blot-119202416527..gif "<b>Genetic Strategies Validation. </b>Western Blot: RHAMM/CD168 Antibody - BSA Free [NBP1-76538] - Loading: 10 μg of lysate Antibodies: RHAMM/CD168 , 1 ug/mL and beta-actin, 1ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.")
![<b>Genetic Strategies Validation. </b>Western Blot: RHAMM/CD168 Antibody [NBP1-76538] - Analysis in rat stomach tissue lysate with antibody at 0.5 ug/mL.](http://images.novusbio.com/fullsize/RHAMM-CD168-Antibody-Western-Blot-NBP1-76538-img0004.jpg)
![Immunohistochemistry: RHAMM/CD168 Antibody [NBP1-76538] - Human stomach tissue.](http://images.novusbio.com/fullsize/RHAMM-CD168-Antibody-Immunohistochemistry-NBP1-76538-img0003.jpg)
![Flow Cytometry: RHAMM/CD168 Antibody [NBP1-76538] - Human megakaryocytes. 47,000 CD41+ CD42+ human iPS-derived megakaryocytes [Moreau et al., 2016] were stained for 20 min at room temperature. Cells were then washed in 500 uL PBS/BSA/EDTA and viable DAPI cells were analysed using a Galios flow cytometer. Flow cytometry image submitted by a verified customer review. Image from the Alexa Fluor 700 version of this antibody.](http://images.novusbio.com/fullsize/RHAMM-CD168-Antibody-Flow-Cytometry-NBP1-76538-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: RHAMM/CD168 Antibody - BSA Free [NBP1-76538] - Immunofluorescence of RHAMM/CD168 in human stomach tissue with RHAM antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76538_rabbit-polyclonal-rhamm-cd168-antibody-2711202420243358.jpg)
![Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-60657] - Lane 1: Goat IgG Load: 50 ug per lane Secondary antibody: Goat IgG (H&L) Antibody DyLight (TM) 488 conjugated Pre-Adsorbed at 1:1,000 for 60 min at RT Block for30 min at RT Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa Image using the DyLight 488 form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP2-60657-img0012.jpg "Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-60657] - Lane 1: Goat IgG Load: 50 ug per lane Secondary antibody: Goat IgG (H&L) Antibody DyLight (TM) 488 conjugated Pre-Adsorbed at 1:1,000 for 60 min at RT Block for30 min at RT Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa Image using the DyLight 488 form of this antibody.")
![Immunocytochemistry/Immunofluorescence: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-60657] - Image shows detection of PD-1 in mouse frozen tissue sections (salivary gland injected with human T cells) using DyLight™ 488 conjugated donkey anti-goat IgG (H+L) secondary antibody (green). Image from verified customer review. Image using the DyLight 488 form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP2-60657-img0007.jpg)
![Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-60657] - Lane 1: Goat IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Texas Red donkey secondary antibody at 1:1,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Goat IgG. Other band(s): None. Image using the Texas Red form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP2-60657-img0001.jpg)
![Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-60657] - Rabbit anti-Transferrin, Goat-anti-Alpha-1-Anti-Trypsin, and Mouse-a-GST. Detection shown was using DyLight549 Donkey anti-Rabbit IgG, DyLight 488 Donkey anti-Mouse IgG, and DyLight 649 Donkey anti-Goat IgG at 1:10000 (in blocking buffer min RT). Blots were washed, rinsed in methanol and dried. Image using the DyLight 649 form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP2-60657-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-60657] - Image shows anti-Histone detection using a DyLight™ 488 conjugate (green). Anti-Tubulin was detected using a DyLight™ 549 conjugate (red). Nuclei were counter-stained using DAPI (blue). Image using the DyLight 488 form of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP2-60657-img0006.jpg)
![Fluorophore-linked immunosorbent assay: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-60657] - Using the DyLight 405 format of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Fluorophore-linked-immunosorbent-assay-NBP2-60657-img0013.jpg)
![ELISA: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP2-60657] - ELISA Results of Donkey Donkey anti-Goat IgG (H+L) Secondary antibody (Pre-adsorbed) tested against purified Goat IgG MX7. Each well was coated in duplicate with 1.0 ug of Goat IgG as well as Chicken IgG, Guinea Porcine IgG, Hamster IgG, Equine IgG, Mouse IgG, Rabbit IgG, and Rat IgG. The working dilution is 1:56,000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer, Goat Anti-Donkey IgG Mx7 HRP conjugated and TMB substrate.](http://images.novusbio.com/fullsize/Donkey-anti-Goat-IgG-H+L-Secondary-Antibody-Pre-adsorbed-ELISA-NBP2-60657-img0014.jpg)
![Western Blot: TLR3 Antibody (27N3D4) [NBP2-27404] - Analysis of TLR3 using TLR3 monoclonal antibody. Human intestine (A), human spleen (B), and mouse RAW lysate (C) probed with TLR3 antibody at 2 ug/ml. I goat anti-rat Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/TLR3-Antibody-27N3D4-Western-Blot-NBP2-27404-img0001.jpg "Western Blot: TLR3 Antibody (27N3D4) [NBP2-27404] - Analysis of TLR3 using TLR3 monoclonal antibody. Human intestine (A), human spleen (B), and mouse RAW lysate (C) probed with TLR3 antibody at 2 ug/ml. I goat anti-rat Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Flow Cytometry: TLR3 Antibody (27N3D4) [NBP2-27404] - Analysis of TLR3 using TLR3 monoclonal antibody. Mouse splenocytes were probed with TLR3 antibody (red) and isotype control antibody (green) at 1 ug/10^6 cells.](http://images.novusbio.com/fullsize/TLR3-Antibody-27N3D4-Flow-Cytometry-NBP2-27404-img0009.jpg)
![Flow Cytometry: TLR3 Antibody (27N3D4) [NBP2-27404] - Analysis using Azide Free version of NBP2-27404. TLR3 using TLR3 monoclonal antibody. Mouse RAW cells and splenocytes were probed with TLR3 antibody (red) and isotype control antibody (green) at 1 ug/10^6 cells.](http://images.novusbio.com/fullsize/TLR3-Antibody-27N3D4-Flow-Cytometry-NBP2-27404-img0006.jpg)
![Flow Cytometry: TLR3 Antibody (27N3D4) [NBP2-27404] - Analysis using Azide Free version of NBP2-27404. TLR3 using TLR3 monoclonal antibody. Mouse RAW cells were probed with TLR3 antibody (red) and isotype control antibody (green) at 0.1 ug/10^6 cells.](http://images.novusbio.com/fullsize/TLR3-Antibody-27N3D4-Flow-Cytometry-NBP2-27404-img0007.jpg)
![Flow Cytometry: TLR3 Antibody (27N3D4) [NBP2-27404] - Analysis of TLR3 using TLR3 monoclonal antibody. Mouse RAW cells were probed with TLR3 antibody (red) and isotype control antibody (green) at 1 ug/10^6 cells.](http://images.novusbio.com/fullsize/TLR3-Antibody-27N3D4-Flow-Cytometry-NBP2-27404-img0008.jpg)
![Flow Cytometry: TLR3 Antibody (27N3D4) - BSA Free [NBP2-27404] - An intracellular stain was performed on THP-1 cells with TLR3 Antibody [27N3D4] NBP2-27405AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.](http://images.novusbio.com/fullsize/nbp2-27404_rat-monoclonal-tlr3-antibody-27n3d4-177202495942.jpg)
![Flow Cytometry: TLR3 Antibody (27N3D4) - BSA Free [NBP2-27404] - An intracellular stain was performed on Raw264.7 cells with TLR3 [27N3D4] Antibody NBP2-27405AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.](http://images.novusbio.com/fullsize/nbp2-27404_rat-monoclonal-tlr3-antibody-27n3d4-17720241044614.jpg)
![Immunohistochemistry-Paraffin: Myelin PLP Antibody [NBP1-87781] - Staining in human cerebral cortex and pancreas tissues using anti-PLP1 antibody. Corresponding PLP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Myelin-PLP-Antibody-Immunohistochemistry-Paraffin-NBP1-87781-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Myelin PLP Antibody [NBP1-87781] - Staining in human cerebral cortex and pancreas tissues using anti-PLP1 antibody. Corresponding PLP1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Myelin PLP Antibody [NBP1-87781] - Border of a demyelinated lesion. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Myelin-PLP-Antibody-Immunohistochemistry-Paraffin-NBP1-87781-img0013.jpg)
![Immunohistochemistry-Paraffin: Myelin PLP Antibody [NBP1-87781] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/Myelin-PLP-Antibody-Immunohistochemistry-Paraffin-NBP1-87781-img0010.jpg)
![Immunohistochemistry-Paraffin: Myelin PLP Antibody [NBP1-87781] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Myelin-PLP-Antibody-Immunohistochemistry-Paraffin-NBP1-87781-img0011.jpg)