Goat anti-Mouse IgG2a Heavy Chain Secondary Antibody

• Reactivity: Mu
• Applications: WB, ELISA, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Goat anti-Mouse IgG2a Heavy Chain Secondary Antibody Summary

• Immunogen: Mouse IgG2a
• Specificity: By immunoelectrophoresis and ELISA this reacts specifically with mouse IgG2a. This may cross react with IgG2a from other species
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000-1:30000(Primary) 1:100-1:500(for coating plates)
  • Immunocytochemistry/Immunofluorescence 1:200- 1:2000
  • Immunohistochemistry 1:200- 1:2000
  • Immunohistochemistry-Paraffin 1:200- 1:2000
  • Western Blot 1:1000-1:30000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG2a Antibody (NB7513)(13)

Publications using NB7513

• Choi JH, Kim JY, Yi MH Et al. Anisakis pegreffii Extract Induces Airway Inflammation with Airway Remodeling in a Murine Model System Biomed Res Int 2021-09-28 [PMID: 34580637] (ELISA)
  
  Details:
  Citation using the Biotin version of this antibody.
• Li Z, Cao Y, Li Y et al. Vaccine delivery alerts innate immune systems for more immunogenic vaccination JCI Insight 2021-03-10 [PMID: 33690222] (Mouse)
  
  Details:
  Citation using the HRP format of this antibody.
• Rodriguez-Morales O, Cabrera-Mata JJ, Carrillo-Sanchez SDC et al Electrolyzed Oxidizing Water Modulates the Immune Response in BALB/c Mice Experimentally Infected with Trypanosoma cruzi Pathogens 2020-11-26 [PMID: 33238401]
  
  Details:
  Citation using the HRP version of this antibody.
• Machahua C, Montes-Worboys A, Llatjos R et al. Increased AGE-RAGE ratio in idiopathic pulmonary fibrosis Respir Res 2016-11-07 [PMID: 27816054]
• Hayashi T, Murase A. Polarization toward Th1-type response in active, but not in inactive, lupus inhibits late allergic rhinitis in lupus-prone female NZBxNZWF(1) mice. Inflammation. [PMID: 22743757]
  
  Details:
  Citation using the FITC form of this antibody.
• Burbach BJ, Srivastava R, Medeiros RB et al. Distinct regulation of integrin-dependent T cell conjugate formation and NF-kappa B activation by the adapter protein ADAP. J Immunol 2008-10-01 [PMID: 18802088]
• Ehlers M, Fukuyama H, McGaha TL et al. TLR9/MyD88 signaling is required for class switching to pathogenic IgG2a and 2b autoantibodies in SLE. J Exp Med. [PMID: 16492804]
  
  Details:
  Citation using the HRP form of this antibody.
• Shimizu S, Sugiyama N, Masutani K et al. Membranous glomerulonephritis development with Th2-type immune deviations in MRL/lpr mice deficient for IL-27 receptor (WSX-1). J Immunol 2005-12-01 [PMID: 16301622]
• Miyazaki Y, Inoue H, Matsumura M et al. Exacerbation of experimental allergic asthma by augmented Th2 responses in WSX-1-deficient mice. J Immunol 2005-08-01 [PMID: 16081811]
• Bonanni D, Rindi L, Lari N et al. Immunogenicity of mycobacterial PPE44 (Rv2770c) in Mycobacterium bovis BCG-infected mice. J Med Microbiol 2005-05-01 [PMID: 15824420]
• Goodfellow JA, Bowes T, Sheikh K et al. Overexpression of GD1a ganglioside sensitizes motor nerve terminals to anti-GD1a antibody-mediated injury in a model of acute motor axonal neuropathy. J Neurosci. [PMID: 15716397]
  
  Details:
  Citation using the FITC form of this antibody.
• Fukuyama H, Nimmerjahn F, Ravetch JV et al. The inhibitory Fcgamma receptor modulates autoimmunity by limiting the accumulation of immunoglobulin G+ anti-DNA plasma cells. Nat Immunol. [PMID: 15592473]
  
  Details:
  Citation using the HRP form of this antibody.
• Shimizu S, Nakashima H, Masutani K et al. Anti-monocyte chemoattractant protein-1 gene therapy attenuates nephritis in MRL/lpr mice. Rheumatology (Oxford) 2004-09-01 [PMID: 15213333]