Immunology reagents: Novus Biologicals

NBP1-76769

PD-L1 Antibody - BSA Free

(5 Reviews)

(42 Publications)

0.1 mg

NBP1-76769

Immunohistochemistry-Paraffin: PD-L1 Antibody [NBP1-76769] - CD274 (PD-L1) expression in the HNSCC patients from the TCGA database , HNSCC tissue samples and the HNSCC cells. Immunostaining of PD-L1 obtained from HNSCC tumor cells, immune cells and tumor margin tissues in HNSCC tissue samples (magnification A-200, scale bars 50AI1/4m) : low tumor staining; moderate tumor staining; high tumor staining have been observed. Image collected and cropped by Citeab from the following publication (Lactoferricin B reverses cisplatin resistance in head and neck squamous cell carcinoma cells through targeting PD-L1. Cancer Med (2018)) licensed under a CC-BY license.

Western Blot: PD-L1 Antibody [NBP1-76769] - PD-L1 expression was evaluated in gastric cancer cells. Levels of PD-L1 protein were assessed in normal human gastric epithelial cells and 8 gastric cancer cell lines by Western blots. Image collected and cropped by Citeab from the following publication (Autophagy inhibition enhances PD-L1 expression in gastric cancer. J Exp Clin Cancer Res (2019)) licensed under a CC-BY license.

<b>Biological Strategies Validation. </b>Western Blot: PD-L1 Antibody [NBP1-76769] - Upregulated expression of PD-L1 in cisplatin-resistant cells through IL-6/STAT3. Image collected and cropped by Citeab from the following publication (Lactoferricin B reverses cisplatin resistance in head and neck squamous cell carcinoma cells through targeting PD-L1. Cancer Med (2018)) licensed under a CC-BY license.

Western Blot: PD-L1 Antibody [NBP1-76769] - CD274 (PD-L1) expression in the HNSCC patients from the TCGA databas HNSCC cells. CD274 expression with survival and its relation with therapy in the HNSCC patients from the TCGA database. Expression of CD274 gene and PD-L1 protein in the established cisplatin-resistant HNSCC cells and cisplatin sensitive cells by qRT-PCR and WB (F, H): CD274 (PD-L1) expressed in cisplatin resistant cell. Image collected and cropped by Citeab from the following publication (Lactoferricin B reverses cisplatin resistance in head and neck squamous cell carcinoma cells through targeting PD-L1. Cancer Med (2018)) licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: PD-L1 Antibody [NBP1-76769] - U-251 MG cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NBP1-76769 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Flow Cytometry: PD-L1 Antibody [NBP1-76769] - An intracellular stain was performed on U-251 MG cells with PD-L1 Antibody NBP1-76769 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

Western Blot: PD-L1 Antibody [NBP1-76769] - Expression of CD274 in Human melanoma SK-MEL-28 cell line upon treatment with 200 mM ethanol. Dilution: 1:1,000 in PBS with 5% BSA. Secondary Ab: anti-Rabbit IgG 1:5,000. Western blot image submitted by a verified customer review.

Immunohistochemistry-Paraffin: PD-L1 Antibody [NBP1-76769] - B7-H1/PD-L1/CD274 Antibody [NBP1-76769] - Human tonsil tissue with PD-L1 antibody at 5 ug/mL.

Immunohistochemistry-Paraffin: PD-L1 Antibody [NBP1-76769] - Staining of human heart tissue with antibody at 2.5 ug/mL.

Immunohistochemistry-Paraffin: PD-L1 Antibody [NBP1-76769] - PD-L1/B7-H1 Antibody [NBP1-76769] - PD-L1 in rat heart tissue with at 5 ug/mL.

Flow Cytometry: PD-L1 Antibody [NBP1-76769] - B7-H1/PD-L1/CD274 Antibody [NBP1-76769] - Analysis of A-20 cells using B7-H1/PD-L1/CD274 antibody at 0.5 ug/mL. Green: Isotype control. Red : B7-H1/PD-L1/CD274 antibody.

Immunofluorescence: PD-L1 Antibody [NBP1-76769] - Human Heart cells with PD-L1 antibody at 20 ug/mL.

Dual RNAscope ISH-IHC: PD-L1 Antibody [NBP1-76769] - PDCD1 mRNA (red) and PD-L1 protein (green) were detected in formalin-fixed paraffin-embedded tissue sections of human lung cancer. ACD's Integrated Co-Detection Workflow was performed using ACD RNAScope Probe Hs-PDCD1 and PD-L1 antibody at 1:100 dilution. Tissue was stained on Leica Bond RX using RNAscope (TM) 2.5 LS Reagent Kit-RED, BOND Polymer Refine Detection (DAB) and Hematoxylin, BOND Polymer Refine Red Detection and Hematoxylin and RNAscope (TM) 2.5 LS Green Accessory Pack. Tissue was counterstained with 50% hematoxylin (blue).

Abrogation of autophagy by siRNAs targeting autophagy-related genes in gastric cancer cells induced tumor-intrinsic PD-L1 expression. a Inhibition of autophagy by knockdown of ATG5 in AGS & NCI-n87 gastric cancer cells induced the expression of PD-L1 in the presence & absence of INF-gamma (200 U/mL) as shown by flow cytometry analysis at 48 h post-transfection. b The induction of PD-L1 was confirmed by Western blots at 72 h post-transfection. The knockdown efficacies of ATG5 & ATG7 siRNA were verified. The conversion of LC3B-I to LC3B-II was reduced. Results were averaged & blots were representative of 4 independent experiments. The ratio of PD-L1 MFI minus isotype control was shown as mean ± S.D. relative to Ctrl from 4 independent experiments, *p < 0.05 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30925913), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Effects of autophagy inhibitors in combination with IFN-gamma on expression of PD-L1 in gastric cancer cell lines. a The effect of chloroquine (CQ) or 3-MA on expression of PD-L1 with or without INF-gamma for 24 h was determined by flow cytometry assays. In AGS & NCI-n87 cells, MFI as the indication of PD-L1 expression level can be further increased by the treatment of INF-gamma . b Levels of LC3B-I/II, p62/SQSTM1 & PD-L1 were determined by Western blots in AGS & NCI-n87 cells treated by CQ, 3-MA, bafilomycin A1 (Baf) or rapamycin (Rap) for 24 h. c Positive staining of PD-L1 (red) & LC3 positive puncta (green) was determined by immunofluorescence in AGS & NCI-n87 cells treated by autophagy inhibitors & activator as in (b). d Rapamycin decreased the levels of PD-L1 protein in AGS & NCI-n87 cells as shown by flow cytometry. Results were averaged & blots were representative of 4 independent experiments, *p < 0.05, **p < 0.01 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30925913), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: PD-L1 Antibody - BSA Free [NBP1-76769] - ROS mediated PD-L1 upregulation in FFA-treated LO2 cells. (a) Intracellular ROS in LO2 cells before & after FFA treatment measured by DCFH-DA. (b–d) Expression levels of NOX2 & NOX4 measured using qRT-PCR & western blot. (e) JC-1 probes were used to detect mitochondrial membrane potential. (f–j) Intracellular ROS in LO2 cells pretreated with siRNA against NOX4, MitoTEMPO (10 μM), or NAC (5 mM) followed by FFA treatment, measured by DCFH-DA, PD-L1 expression determined by western blot. ∗∗P < 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35615575), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: PD-L1 Antibody - BSA Free [NBP1-76769] - Inhibition of NF-kappa B or STAT3 downregulated PD-L1 expression in adi-CM model. a PD-L1 protein levels in HepG2 cells treated with different concentration of NF-kappa B inhibitor, withaferin A (WA). b PD-L1 protein levels in HepG2 cells treated with different concentration of STAT3 inhibitor, BP-1-102. c PD-L1 protein levels in B16-F1 cells incubated with WA. Data are expressed as mean ± SEM, n = 6, **P < 0.01, ***P < 0.001 Vs adi-CM group. ##P < 0.01 & ###P < 0.001 Vs DMSO group Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32477009), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: PD-L1 Antibody - BSA Free [NBP1-76769] - PD-L1 expression levels in liver samples & FFA-treated LO2 cells. (a–d) PD-L1 expression & CD8+ T cells in the liver tissues determined by immunohistochemistry. (e) Intracellular lipid accumulation after FFA treatment (0.8 mM) for 24 hour measured by Oil Red staining. (f–i) Expression levels of PD-L1 detected by qRT-PCR, western blot, & immunofluorescence. ∗∗P < 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35615575), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: PD-L1 Antibody - BSA Free [NBP1-76769] - ROS/ZNF24/PD-L1 pathway activation in NAFLD models. (a) Intracellular ROS in hepatocytes in NAFLD significantly increased. (b) JC-1 probes were used to detect mitochondrial membrane potential. (c & d) NOX4 & ZNF24 expressions were determined by immunohistochemistry. (e & f) ROS/ZNF24/PD-L1 pathway activation examined by western blot. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35615575), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: PD-L1 Antibody - BSA Free [NBP1-76769] - PD-L1 limits liver injury in NAFLD models. (a & b) Western blot determined the effect of siRNA against PD-L1. (c) After PD-L1 knockdown, mRNA expression levels of markers of T cell activation measured by qRT-PCR. (d & e) Hepatocyte injury was evaluated by the Tunel assay & ALT/AST measurement. (f) Graph illustrating that both ROS/ZNF24 pathway activation & UBE2I-mediated ZNF24 sumoylation suppression induced by FFA promoted PD-L1 expression. ∗P < 0.05, ∗∗P < 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35615575), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: PD-L1 Antibody - BSA Free [NBP1-76769] - PD-L1 knockdown aggravated the damage of CD8 + T cells to FFA-treated LO2 cells. (a & b) Western blot determined the effect of siRNA against PD-L1. Coculturing of FFA-treated LO2 cells & CD8 + T cells in vitro. (c) mRNA expression levels of markers of T cell activation measured by qRT-PCR, (d) LO2 cell injury was evaluated by LDH assay, (e) AST or ALT in the supernatants measured by commercial assay kits. # represents that CD8 + T cells were incubated separately with LO2 cells, but supernatants were put together. ∗P < 0.05, ∗∗P < 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35615575), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: PD-L1 Antibody - BSA Free [NBP1-76769] - Upregulated expression of PD‐L1 in cisplatin‐resistant cells through IL‐6/STAT3. The cisplatin resistant CAL27cis & Detroit‐562cis cells had increased IL‐6 mRNA & IL‐6 protein production compared to parental cells by qRT‐PCR & ELISA (A, B). CAL27cis & Detroit‐562cis were cultured with IL‐6 (10 ng/mL, human recombinant IL‐6, R&D Systems, Minneapolis, USA) for 48 h (C). An anti‐human IL‐6 polyclonal antibody (R&D Systems, Minneapolis, USA) was used to neutralize the biological activities of IL‐6 (E, F). To inhibit signal transduction, CAL27cis & Detroit‐562cis cells were pre‐incubated for 1 h with STAT3 inhibitor V (Sigma‐Aldrich, Germany, 500 nmol/L), & then incubated with recombinant human IL‐6 (10 ng/mL) for 24 h before analysis. Data are shown as mean ± SD of 3 independent triplicate cultures (A, B, D, E, F, H). *P < .05, **P < .01 & ***P < .001, compared with the results of control without IL‐6 (D), control IgG (E, F) & with IL‐6 alone (H) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29761938), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: PD-L1 Antibody - BSA Free [NBP1-76769] - ZNF24 promoted PD-L1 expression through binding to its promoter in FFA-treated LO2 cells. (a) A schematic of the target sites (wild & mutant) of ZNF24 in the promoter of PD-L1. (b–d) Dual-luciferase reporter assays performed in LO2 cells transfected with WT or MT plasmid containing ZNF24-binding sites in the PD-L1 promoter using Lipofectamine 2000 after ZNF24 overexpression. (e–g) ZNF24 expression after FFA treatment determined by qRT-PCR & western blot. (h & i) ZNF24 expression in LO2 cells pretreated with siRNA against NOX4, MitoTEMPO (10 μM), or NAC (5 mM) followed by FFA treatment, measured by western blot. (j & k) ZNF24 & PD-L1 expression in LO2 cells pretreated with siRNA against ZNF24, followed by FFA treatment detected by western blot. ∗∗P < 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35615575), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: PD-L1 Antibody - BSA Free [NBP1-76769] - LfcinB reduced IL‐6‐dependent PD‐L1 expression in cisplatin‐resistant HNSCC cells (A‐C) & inhibited the xenograft growth from CAL27cis & Detroit‐562cis (D, E). The markedly decreased IL‐6 (A) by ELISA & PD‐L1 by WB (B, C) in the acquired cisplatin‐resistant cells were detected. In figure A, C & D, data are shown as mean ± SD of 3 independent triplicate cultures. *P < .05 & **P < .01, compared with the results of control without IL‐6 (A, C). To investigate the effects of LfcinB on in vivo growth of CAL27cis & Detroit‐562cis, we treated nude mice carrying CAL27cis (D) & Detroit‐562cis (E) xenografts with once‐daily injection of 0.75 mg LfcinB (n = 5). Tumor growth of HNSCC xenografts displayed significant reduction after 3 days of LfcinB treatment, compared to control with saline (*P < .05 for LfcinB) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29761938), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: PD-L1 Antibody - BSA Free [NBP1-76769] - Identification of ZNF24–UBE2I protein interactions. (a & b) Analysis of protein interactions between ZNF24 & UBE2I using the BioGRID database, further confirmed by Co-IP assays. (c–f) UBE2I expression in LO2 cells treated by FFA or pretreated with siRNA against NOX4, MitoTEMPO (10 μM), or NAC (5 mM) followed by FFA treatment measured by western blot. (g & h) After FFA treatment, Sumo-1: ZNF24 & ZNF24 expression levels detected by western blot. (i & j) After UBE2I overexpression, Sumo-1: ZNF24, ZNF24, PD-L1, & UBE2I expression levels were determined by western blot. (k–m) Dual-luciferase reporter assays performed in LO2 cells transfected with WT plasmid containing ZNF24-binding sites in the PD-L1 promoter using Lipofectamine 2000 after ZNF24 overexpression with or without Sumo-1 overexpression. ∗∗P < 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35615575), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

PD-L1 was detected in immersion fixed U-2 OS human osteosarcoma cell line using Rabbit anti-PD-L1 Antigen Affinity Purified Polyclonal Antibody conjugated to Alexa Fluor® 647 (Catalog # NBP1-76769AF647) (light blue) at 10 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , , , , , ,

Applications: , , , , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-76769AF350
Alexa Fluor 405	NBP1-76769AF405
Alexa Fluor 488	NBP1-76769AF488
Alexa Fluor 532	NBP1-76769AF532
Alexa Fluor 594	NBP1-76769AF594
Alexa Fluor 647	NBP1-76769AF647
Alexa Fluor 700	NBP1-76769AF700
Alexa Fluor 750	NBP1-76769AF750
Allophycocyanin	NBP1-76769APC
Biotin	NBP1-76769B
CoraFluor 1	NBP1-76769CL1
DyLight 350	NBP1-76769UV
DyLight 405	NBP1-76769V
DyLight 488	NBP1-76769G
DyLight 550	NBP1-76769R
DyLight 594	NBP1-76769DL594
DyLight 650	NBP1-76769C
DyLight 680	NBP1-76769FR
DyLight 755	NBP1-76769IR
FITC	NBP1-76769F
HRP	NBP1-76769H
Janelia Fluor 525	NBP1-76769JF525
Janelia Fluor 549	NBP1-76769JF549
Janelia Fluor 646	NBP1-76769JF646
Janelia Fluor 669	NBP1-76769JF669
PE	NBP1-76769PE
PerCP	NBP1-76769PCP
mFluor Violet 450 SE	NBP1-76769MFV450
mFluor Violet 500 SE	NBP1-76769MFV500
mFluor Violet 610 SE	NBP1-76769MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-76769SS

compare

(select up to 3 products from this page to compare)

NB600-1441

CD3 Antibody (SP7)

(3 Reviews)

(50 Publications)

0.5 ml

NB600-1441

<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: CD3 Antibody (SP7) [NB600-1441] - Mouse lung tissue stained against C3aR normal conditions (left) and knockdown conditions (right). IHC-P image submitted by a verified customer review.

Reactivity:

Hu, Mu, Po, Ca

Species Glossary

Reactivity: Hu, Mu, Po, Ca

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

compare

(select up to 3 products from this page to compare)

NBP1-76701

TIM-1/KIM-1/HAVCR Antibody - BSA Free

(38 Publications)

0.1 mg

NBP1-76701

Western Blot: TIM-1/KIM-1/HAVCR Antibody - BSA Free [NBP1-76701] - TIM-1/KIM-1/HAVCR Antibody [NBP1-76701] - Loading: 15 ug of NIH/3T3 cell lysates. Antibodies: TIM-1 3809 (8 ug/mL), overnight incubation at 4 C in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

<b>Independent Antibodies Validation. </b>Western Blot: TIM-1/KIM-1/HAVCR Antibody - BSA Free [NBP1-76701] - TIM-1/KIM-1/HAVCR Antibody [NBP1-76701] - Loading: 15 ug of lysates per lane. Antibodies: TIM-1 3809 (8 ug/mL), TIM-13811 (1 ug/mL), beta-actin (1 ug/mL), and GAPDH (0.02 ug/mL), overnightincubation at 4C (3809) or 1h incubation at RT in 5% NFDM/TBST. Secondary:Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

<b>Genetic Strategies Validation. </b>Western Blot: TIM-1/KIM-1/HAVCR Antibody - BSA Free [NBP1-76701] - TIM-1/KIM-1/HAVCR Antibody [NBP1-76701] - HeLa cells were transfected with control siRNAs (lane 1) or TIM-1 siRNAs (lane 2) Loading: 15 ug of HeLa whole cell lysates per lane. Antibodies: 3809 (8 ug/mL), 1 hincubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot: TIM-1/KIM-1/HAVCR Antibody - BSA Free [NBP1-76701] - Figure 1 Western Blot Validation in Human Cell LinesLoading: 15 u of lysates per lane.Antibodies: TIM-1 (8 u/mL), overnight incubation at 4; C in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunocytochemistry/ Immunofluorescence: TIM-1/KIM-1/HAVCR Antibody - BSA Free [NBP1-76701] - Validation of TIM-1/KIM-1/HAVCR in Human Uterus Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed human uterus cells labeling TIM-1/KIM-1/HAVCR with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing both membrane and cytoplasmic staining on human uterus cells.

KIM1 mediates the cell entry of SARS-CoV-2-RBD. (A) Representative images and quantitative data of cell surface attachment of SARS-CoV-2-RBD in wild-type (WT) and KIM1 knockout (KIM1-KO) HK-2 cells. Scale bar, 20 μm. ***P < 0.001. (B) Representative images and quantitative data of cell surface attachment of SARS-CoV-2-RBD in HEK293T cells. Scale bar, 20 μm. OE KIM1, overexpression of KIM1. **P < 0.01. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/33493263), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-76701AF350
Alexa Fluor 405	NBP1-76701AF405
Alexa Fluor 488	NBP1-76701AF488
Alexa Fluor 532	NBP1-76701AF532
Alexa Fluor 594	NBP1-76701AF594
Alexa Fluor 647	NBP1-76701AF647
Alexa Fluor 700	NBP1-76701AF700
Alexa Fluor 750	NBP1-76701AF750
Allophycocyanin	NBP1-76701APC
Biotin	NBP1-76701B
CoraFluor 1	NBP1-76701CL1
DyLight 350	NBP1-76701UV
DyLight 405	NBP1-76701V
DyLight 488	NBP1-76701G
DyLight 550	NBP1-76701R
DyLight 594	NBP1-76701DL594
DyLight 650	NBP1-76701C
DyLight 680	NBP1-76701FR
DyLight 755	NBP1-76701IR
FITC	NBP1-76701F
HRP	NBP1-76701H
Janelia Fluor 525	NBP1-76701JF525
Janelia Fluor 549	NBP1-76701JF549
Janelia Fluor 646	NBP1-76701JF646
Janelia Fluor 669	NBP1-76701JF669
PE	NBP1-76701PE
PerCP	NBP1-76701PCP
mFluor Violet 450 SE	NBP1-76701MFV450
mFluor Violet 500 SE	NBP1-76701MFV500
mFluor Violet 610 SE	NBP1-76701MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-76701SS

compare

(select up to 3 products from this page to compare)

NB300-504

NFATC2/NFAT1 Antibody (25A10.D6.D2) - BSA Free

(14 Publications)

100 μg

NB300-504

Chromatin Immunoprecipitation: NFATC2/NFAT1 Antibody (25A10.D6.D2) [NB300-504] - IP performed using Matrix ChIP assay with 1.0ul/100ul well of NFATc2 mab. Chromatin aliquots from ~1x10^5 cells used per ChIP pull-down. Quantitative PCR data done x4 using 1ul of eluted DNA in 2ul SYBR real-time PCR reactions containing primers to amplify exon-1 or exon-28 of LAMC1. PCR calibration curves generated per primer pair from a series of sheared total genomic DNA. ChIP presented relative to the total amount of input chromatin. Results represent mean +/- SEM for 3 experiments. Representation of rat LAMC1 locus shown above data where boxes represent exons (black = translated regions, white = untranslated regions); zigzag line represents an intron, & straight line represents upstream sequence. Regions amplified by LAMC1 primers are represented by black bars. Data courtesy of the Innovators Program.

Immunocytochemistry/Immunofluorescence: NFATC2/NFAT1 Antibody (25A10.D6.D2) [NB300-504] - Analysis of NFATc2 using NFATc2 Monoclonal Antibody (25A10.D6.D2) shows staining in Hela Cells. NFATc2 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing NFATc2 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Immunohistochemistry-Paraffin: NFATC2/NFAT1 Antibody (25A10.D6.D2) [NB300-504] - Cancer biopsies of deparaffinized Human colon carcinoma tissues.

Immunohistochemistry-Paraffin: NFATC2/NFAT1 Antibody (25A10.D6.D2) [NB300-504] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB300-504AF350
Alexa Fluor 405	NB300-504AF405
Alexa Fluor 488	NB300-504AF488
Alexa Fluor 532	NB300-504AF532
Alexa Fluor 594	NB300-504AF594
Alexa Fluor 647	NB300-504AF647
Alexa Fluor 700	NB300-504AF700
Alexa Fluor 750	NB300-504AF750
Allophycocyanin	NB300-504APC
Biotin	NB300-504B
CoraFluor 1	NB300-504CL1
DyLight 350	NB300-504UV
DyLight 405	NB300-504V
DyLight 488	NB300-504G
DyLight 550	NB300-504R
DyLight 594	NB300-504DL594
DyLight 650	NB300-504C
DyLight 680	NB300-504FR
DyLight 755	NB300-504IR
FITC	NB300-504F
HRP	NB300-504H
Janelia Fluor 525	NB300-504JF525
Janelia Fluor 549	NB300-504JF549
Janelia Fluor 646	NB300-504JF646
Janelia Fluor 669	NB300-504JF669
PE	NB300-504PE
PerCP	NB300-504PCP
mFluor Violet 450 SE	NB300-504MFV450
mFluor Violet 500 SE	NB300-504MFV500
mFluor Violet 610 SE	NB300-504MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB300-504-0.025mg

compare

(select up to 3 products from this page to compare)

NBP2-25196

CD19 Antibody (CB19) - BSA Free

(1 Review)

(12 Publications)

0.1 mg

NBP2-25196

Immunocytochemistry/Immunofluorescence: CD19 Antibody (CB19) [NBP2-25196] - The CD19 (CB19) antibody was tested in Daudi cells at a 1:40 dilution against Dylight 488 (Green). Actin and nuclei were counterstained against Phalloidin 568 (Red) and DAPI (Blue), respectively.

Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - Surface staining of 10^6 human lymphocytes using 0.2 ug of CD19 antibody (red) and isotype control (green). Cell surface staining kit was used for this test.

Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - Analysis using the FITC conjugate of NBP2-25196. Staining of CD19 in 10^6 human lymphocytes using 10 ul (0.25 ug) of this antibody. Shaded histogram represents cells without antibody; green represents isotype control ; red represents anti-CD19 antibody.

Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - Analysis using the PE conjugate of NBP2-25196. Staining of CD19 in 1x10^6 human PBMC using 10 ul (0.25 ug) of was used to test this product. Propidium iodide negative lymphocyte population gated for analysis.

Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - Analysis using the Alexa Fluor (R) 700 conjugate of NBP2-25196. Staining of human PBMC. Image from verified customer review.

Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - Analysis using the DyLight 405 conjugate of NBP2-25196. Staining of CD19 in human PBMCs using anti-CD19 antibody. Image from verified customer review.

Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - A surface stain was performed on human peripheral blood lymphocytes with TACI/TNFRSF13B/CVID (1A1) antibody NB600-1189PE and a matched isotype control. Cells were incubated in an antibody dilution of 2 ug/mL for 20 minutes at room temperature. A co-stain was performed with NBP2-25196AF647.

Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - A surface stain was performed on human peripheral blood lymphocytes with CD9 (HI9a) antibody NB100-77915AF647 and a matched isotype control NBP2-27287AF488. Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. A co-stain was performed with NBP2-26646.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Alt Catalog#

IMG-6321A

Alt Catalog# IMG-6321A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-25196AF350
Alexa Fluor 405	NBP2-25196AF405
Alexa Fluor 488	NBP2-25196AF488
Alexa Fluor 532	NBP2-25196AF532
Alexa Fluor 594	NBP2-25196AF594
Alexa Fluor 647	NBP2-25196AF647
Alexa Fluor 700	NBP2-25196AF700
Alexa Fluor 750	NBP2-25196AF750
Allophycocyanin	NBP2-25196APC
Allophycocyanin/Cy7	NBP2-25196APCCY7
Biotin	NBP2-25196B
CoraFluor 1	NBP2-25196CL1
DyLight 350	NBP2-25196UV
DyLight 405	NBP2-25196V
DyLight 488	NBP2-25196G
DyLight 550	NBP2-25196R
DyLight 594	NBP2-25196DL594
DyLight 650	NBP2-25196C
DyLight 680	NBP2-25196FR
DyLight 755	NBP2-25196IR
FITC	NBP2-25196F
HRP	NBP2-25196H
Janelia Fluor 525	NBP2-25196JF525
Janelia Fluor 549	NBP2-25196JF549
Janelia Fluor 646	NBP2-25196JF646
Janelia Fluor 669	NBP2-25196JF669
PE	NBP2-26646
PE/Atto594	NBP2-25196PEATT594
PE/Cy5.5	NBP2-25196PECY55
PE/Cy7	NBP2-25196PECY7
PerCP	NBP2-25196PCP
mFluor Violet 450 SE	NBP2-25196MFV450
mFluor Violet 500 SE	NBP2-25196MFV500
mFluor Violet 610 SE	NBP2-25196MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-25196SS
Azide and BSA Free	NBP2-80634

compare

(select up to 3 products from this page to compare)

NBP2-25186

CD3 Antibody (OKT3) - BSA Free

(10 Publications)

0.1 mg

NBP2-25186

Flow Cytometry: CD3 Antibody (OKT3) [NBP2-25186] - Analysis using Azide Free version of NBP2-25186. CD3+ hPMBCs using the APC conjugate of this antibody. A cell surface stain was performed on CD3+ hPBMCs with V alpha 24 J alpha 18 TCR antibody (6B11) NBP2-00267PE (top image) and a matched isotype control

Flow Cytometry: CD3 Antibody (OKT3) [NBP2-25186] - Analysis of CD3 expression in hPBMC's gated to lymphocyte population at 1 x 10^6 cells/mL. Cells were stained with NBP2-24867PE (orange) conjugate at a 1:500 dilution. Results are shown with mIgG (PE conjugate) isotype control (blue). Image using the PE format of this antibody.

Flow Cytometry: CD3 Antibody (OKT3) [NBP2-25186] - Analysis of PerCP conjugate of NBP2-25186. A surface stain was performed on Jurkat cells with CD3 (OKT3) antibody NBP2-24867PCP (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room

Flow Cytometry: CD3 Antibody (OKT3) [NBP2-25186] - Analysis using the Alexa Fluor(R) 488 conjugate of NBP2-24867. Detection of CD3 expression in hPBMc's gated to lymphocyte population at 1 x 10^6 cells/mL. Cells were stained with NBP2-24867AF488 (orange) conjugate at a 1:500 dilution. Results are shown with mIgG2 (AF488 conjugate) isotype control (blue). Image using the Low Endotoxin and Azide Free form of this antibody.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Alt Catalog#

IMG-6240A

Alt Catalog# IMG-6240A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-24867AF350
Alexa Fluor 405	NBP2-24867AF405
Alexa Fluor 488	NBP2-24867AF488
Alexa Fluor 532	NBP2-24867AF532
Alexa Fluor 594	NBP2-24867AF594
Alexa Fluor 647	NBP2-24867AF647
Alexa Fluor 700	NBP2-24867AF700
Alexa Fluor 750	NBP2-24867AF750
Allophycocyanin	NBP2-24867APC
DyLight 350	NBP2-24867UV
DyLight 405	NBP2-24867V
DyLight 488	NBP2-24867G
DyLight 550	NBP2-24867R
DyLight 594	NBP2-25186DL594
DyLight 650	NBP2-24867C
DyLight 680	NBP2-24867FR
DyLight 755	NBP2-24867IR
FITC	NBP2-24867F
Janelia Fluor 549	NBP2-24867JF549
Janelia Fluor 646	NBP2-24867JF646
PE	NBP2-24867PE
PerCP	NBP2-24867PCP
mFluor Violet 450 SE	NBP2-24867MFV450
mFluor Violet 500 SE	NBP2-24867MFV500
mFluor Violet 610 SE	NBP2-24867MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Low Endotoxin, Azide and BSA Free	NBP2-24867
BSA Free	NBP2-25186SS
Low Endotoxin, Azide and BSA Free	NBP2-24867SS

compare

(select up to 3 products from this page to compare)

NBP1-43262

PD-L1 Antibody (MIH5) - BSA Free

(1 Review)

(11 Publications)

0.1 mg

NBP1-43262

Flow Cytometry: PD-L1/B7-H1 Antibody (MIH5) [NBP1-43262] - B7-H1/PD-L1/CD274 Antibody (MIH5) [NBP1-43262] - Staining of mouse splenocytes with Anti-Mouse B7-H1/PD-L1/CD274) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Flow Cytometry: PD-L1/B7-H1 Antibody (MIH5) [NBP1-43262] - Analysis using the DyLight 488 conjugate of NBP1-43262. Staining of PD-L1 in human primary colon cancer cell using anti-PD-L1 antibody. Image from verified customer review.

Flow Cytometry: PD-L1/B7-H1 Antibody (MIH5) [NBP1-43262] - Analysis using the DyLight 488 conjugate of NBP1-43262. Staining of PD-L1 in HT29 cells using anti-PD-L1 antibody. The data shows the detection of total PD-L1 status in the cancer cells. Image from verified customer review.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , , ,

Applications: , , , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-43262AF350
Alexa Fluor 405	NBP1-43262AF405
Alexa Fluor 488	NBP1-43262AF488
Alexa Fluor 532	NBP1-43262AF532
Alexa Fluor 594	NBP1-43262AF594
Alexa Fluor 647	NBP1-43262AF647
Alexa Fluor 700	NBP1-43262AF700
Alexa Fluor 750	NBP1-43262AF750
Allophycocyanin	NBP1-43262APC
Biotin	NBP1-43262B
CoraFluor 1	NBP1-43262CL1
DyLight 350	NBP1-43262UV
DyLight 405	NBP1-43262V
DyLight 488	NBP1-43262G
DyLight 550	NBP1-43262R
DyLight 594	NBP1-43262DL594
DyLight 650	NBP1-43262C
DyLight 680	NBP1-43262FR
DyLight 755	NBP1-43262IR
FITC	NBP1-43262F
HRP	NBP1-43262H
Janelia Fluor 525	NBP1-43262JF525
Janelia Fluor 549	NBP1-43262JF549
Janelia Fluor 646	NBP1-43262JF646
Janelia Fluor 669	NBP1-43262JF669
PE	NBP1-43262PE
PE/Cy5.5	NBP1-43262PECY55
PE/Cy7	NBP1-43262PECY7
PerCP	NBP1-43262PCP
mFluor Violet 450 SE	NBP1-43262MFV450
mFluor Violet 500 SE	NBP1-43262MFV500
mFluor Violet 610 SE	NBP1-43262MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-43262-0.5mg
BSA Free	NBP1-43262-25ug

compare

(select up to 3 products from this page to compare)

NBP2-24965

CD19 Antibody (1D3) - Azide and BSA Free

(3 Publications)

100 μg

NBP2-24965

Flow (Cell Surface): CD19 Antibody (1D3) [NBP2-24965] - Analysis using the Allophycocyanin conjugate of NBP2-24965. Staining of CD19 in BALB/c mouse splenocytes using 1 ug of this antibody. Green represents isotype control; red represents anti-CD19 APC.

Flow Cytometry: CD19 Antibody (1D3) [NBP2-24965] - Analysis using the Allophycocyanin conjugate of NBP2-24965. Staining of CD19 in BALB/c mouse splenocytes. Cells were stained with anti-CD4 PE this antibody and either 1 ug of isotype control antibody (left) or 1 ug of anti-CD19 APC (right).

Flow Cytometry: CD19 Antibody (1D3) [NBP2-24965] - Analysis using the PE conjugate of NBP2-24965. Staining of CD19 in BALB/c mouse splenocytes. Cells were stained with anti-CD4 FITC this antibody and either 1 ug of isotype control antibody (left, ) or 1 ug of anti-CD19 PE (right).

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Alt Catalog#

IMG-6294A

Alt Catalog# IMG-6294A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-24965AF350
Alexa Fluor 405	NBP2-24965AF405
Alexa Fluor 488	NBP2-24965AF488
Alexa Fluor 532	NBP2-24965AF532
Alexa Fluor 594	NBP2-24965AF594
Alexa Fluor 647	NBP2-24965AF647
Alexa Fluor 700	NBP2-24965AF700
Alexa Fluor 750	NBP2-24965AF750
Allophycocyanin	NBP2-24965APC
Biotin	NBP2-24965B
CoraFluor 1	NBP2-24965CL1
DyLight 350	NBP2-24965UV
DyLight 405	NBP2-24965V
DyLight 488	NBP2-24965G
DyLight 550	NBP2-24965R
DyLight 594	NBP2-24965DL594
DyLight 650	NBP2-24965C
DyLight 680	NBP2-24965FR
DyLight 755	NBP2-24965IR
FITC	NBP2-24965F
HRP	NBP2-24965H
Janelia Fluor 525	NBP2-24965JF525
Janelia Fluor 549	NBP2-24965JF549
Janelia Fluor 646	NBP2-24965JF646
Janelia Fluor 669	NBP2-24965JF669
PE	NBP2-24965PE
PerCP	NBP2-24965PCP
mFluor Violet 450 SE	NBP2-24965MFV450
mFluor Violet 500 SE	NBP2-24965MFV500
mFluor Violet 610 SE	NBP2-24965MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-24965SS
Azide and BSA Free	NBP2-80633

compare

(select up to 3 products from this page to compare)

NBP2-53387

CD3 epsilon Antibody (C3e/1308)

(4 Publications)

100 μg

NBP2-53387

Flow Cytometry: CD3 epsilon Antibody (C3e/1308) [NBP2-53387] - Flow Cytometric Analysis of Jurkat cells using CD3 epsilon Antibody (C3e/1308) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Western Blot: CD3 epsilon Antibody (C3e/1308) [NBP2-53387] - Western Blot of recombinant CD3e and Jurkat Cell Lysate using CD3e Monoclonal Antibody (C3e/1308)

Immunohistochemistry-Paraffin: CD3 epsilon Antibody (C3e/1308) [NBP2-53387] - Staining of T cells in human FFPE tonsil tissue using AF647-conjugated CD3 epsilon Antibody (Catalog # NBP2-54392AF647). Antigen Retrieval at pH 9. Antibody incubation over night at 4C. Image from a verified customer review.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-54392AF350
Alexa Fluor 405	NBP2-54392AF405
Alexa Fluor 488	NBP2-54392AF488
Alexa Fluor 532	NBP2-54392AF532
Alexa Fluor 594	NBP2-54392AF594
Alexa Fluor 647	NBP2-54392AF647
Alexa Fluor 700	NBP2-54392AF700
Alexa Fluor 750	NBP2-54392AF750
Allophycocyanin	NBP2-54392APC
Allophycocyanin/Cy7	NBP2-54392APCCY7
Biotin	NBP2-54392B
CoraFluor 1	NBP2-54392CL1
DyLight 350	NBP2-54392UV
DyLight 405	NBP2-54392V
DyLight 488	NBP2-54392G
DyLight 550	NBP2-54392R
DyLight 594	NBP2-54392DL594
DyLight 650	NBP2-54392C
DyLight 680	NBP2-54392FR
DyLight 755	NBP2-54392IR
FITC	NBP2-54392F
HRP	NBP2-54392H
Janelia Fluor 525	NBP2-54392JF525
Janelia Fluor 549	NBP2-54392JF549
Janelia Fluor 646	NBP2-54392JF646
Janelia Fluor 669	NBP2-54392JF669
PE	NBP2-54392PE
PE/Atto594	NBP2-54392PEATT594
PE/Cy5.5	NBP2-54392PECY55
PE/Cy7	NBP2-54392PECY7
PerCP	NBP2-54392PCP
mFluor Violet 450 SE	NBP2-54392MFV450
mFluor Violet 500 SE	NBP2-54392MFV500
mFluor Violet 610 SE	NBP2-54392MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-54392-100ug

compare

(select up to 3 products from this page to compare)

NBP2-15791

PD-L1 Antibody - BSA Free

(3 Publications)

0.1 ml

NBP2-15791

<b>Genetic Strategies Validation. </b>Western Blot: PD-L1 Antibody [NBP2-15791] - PD-L1/B7-H1 Antibody [NBP2-15791] - Non-transfected (-) and transfected (+) A431 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody. HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

<b>Biological Strategies Validation. </b>Western Blot: PD-L1/B7-H1 Antibody [NBP2-15791] - Untreated (-) and treated (+) MDA-MB-231 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody.

Western Blot: PD-L1 Antibody [NBP2-15791] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunocytochemistry/Immunofluorescence: PD-L1 Antibody [NBP2-15791] - MDA-MB-231 (left) and HeLa (right) cells were fixed in ice-cold MeOH for 5 min.Green: PD-L1 stained by PD-L1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.

Immunohistochemistry-Paraffin: PD-L1 Antibody [NBP2-15791] - Human ovarian cancer. PD-L1 stained by PD-L1 antibody diluted at 1:4000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

<b>Independent Antibodies Validation. </b>Western Blot: PD-L1 Antibody [NBP2-15791] - PD-L1/B7-H1 Antibody [NBP2-15791] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membranes were blotted with PD-L1 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. NBP2-15791 on the left and competitor's antibody on the right.

Immunohistochemistry-Paraffin: PD-L1/B7-H1 Antibody [NBP2-15791] - PD-L1 proteinat cell membrane in human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. PD-L1 antibody diluted at 1:1000.

<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PD-L1 Antibody [NBP2-15791] - Detection of cell membranes in PD-L1 protein-expressing cell lines by immunohistochemical analysis. Antibodies: PD-L1 antibody, and competitor's antibody. Samples: Negative (-), low positive (+), intermediate positive (++) and strong positive (+++) cell line cores assessed using Quantitative Digital Pathology.

Western Blot: PD-L1 Antibody [NBP2-15791] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody (NBP2-15791) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

<b>Genetic Strategies Validation. </b>Western Blot: PD-L1 Antibody [NBP2-15791] - Wild-type (WT) and PD-L1 knockout (KO) MDA-MB-231 cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody (NBP2-15791) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

compare

(select up to 3 products from this page to compare)

H00004068-M01

SH2D1A Antibody (1C9) - Azide and BSA Free

(1 Review)

(9 Publications)

0.1 mg

H00004068-M01

Western Blot: SH2D1A Antibody (1C9) [H00004068-M01] - SH2D1A monoclonal antibody (M01), clone 1C9 Analysis of SH2D1A expression in Jurkat.

ELISA: SH2D1A Antibody (1C9) [H00004068-M01] - Detection limit for recombinant GST tagged SH2D1A is 0.3 ng/ml as a capture antibody.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

compare

(select up to 3 products from this page to compare)

NBP2-25200

CD2 Antibody (RPA-2.10) - BSA Free

(1 Review)

0.1 mg

NBP2-25200

Flow Cytometry: CD2 Antibody (RPA-2.10) [NBP2-25200] - Analysis using the Alexa Fluor (R) 488 conjugate of NBP2-25200. Staining of CD2 in 10^6 human lymphocytes using 10 ul (0.25 ug) of this antibody. Shaded histogram represents cells without antibody; green represents isotype control ; red represents anti-CD2.

Flow Cytometry: CD2 Antibody (RPA-2.10) [NBP2-25200] - Analysis using the Allophycocyanin conjugate of NBP2-25200. Staining of normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control APC (open histogram) or Anti-Human CD2 APC (filled histogram). Cells in the lymphocyte gate were used for analysis.

Flow Cytometry: CD2 Antibody (RPA-2.10) [NBP2-25200] - Analysis using the Biotin conjugate of NBP2-25200. Staining of normal human peripheral blood cells with 0.125 ug of Mouse IgG1 kappa Isotype Control Biotin (open histogram) or 0.125 ug of Anti-Human CD2 Biotin (filled histogram) followed by Streptavidin PE.

Flow Cytometry: CD2 Antibody (RPA-2.10) [NBP2-25200] - Analysis using the FITC conjugate of NBP2-25200. Staining of Human peripheral blood lymphocytes with RPA-2.10 FITC.

Flow Cytometry: CD2 Antibody (RPA-2.10) [NBP2-25200] - Analysis using the PE/Cy5 conjugate of NBP2-25200. Staining of normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control PE-Cy5 (open histogram) or Anti-Human CD2 PE-Cy5 (filled histogram). Total cells were used for analysis.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Alt Catalog#

IMG-6296A

Alt Catalog# IMG-6296A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-25200AF350
Alexa Fluor 405	NBP2-25200AF405
Alexa Fluor 488	NBP2-25200AF488
Alexa Fluor 532	NBP2-25200AF532
Alexa Fluor 594	NBP2-25200AF594
Alexa Fluor 647	NBP2-25200AF647
Alexa Fluor 700	NBP2-25200AF700
Alexa Fluor 750	NBP2-25200AF750
Allophycocyanin	NBP2-25200APC
Biotin	NBP2-25200B
CoraFluor 1	NBP2-25200CL1
DyLight 350	NBP2-25200UV
DyLight 405	NBP2-25200V
DyLight 488	NBP2-25200G
DyLight 550	NBP2-25200R
DyLight 594	NBP2-25200DL594
DyLight 650	NBP2-25200C
DyLight 680	NBP2-25200FR
DyLight 755	NBP2-25200IR
FITC	NBP2-25200F
HRP	NBP2-25200H
Janelia Fluor 525	NBP2-25200JF525
Janelia Fluor 549	NBP2-25200JF549
Janelia Fluor 646	NBP2-25200JF646
Janelia Fluor 669	NBP2-25200JF669
PE	NBP2-25200PE
PerCP	NBP2-25200PCP
mFluor Violet 450 SE	NBP2-25200MFV450
mFluor Violet 500 SE	NBP2-25200MFV500
mFluor Violet 610 SE	NBP2-25200MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-25200SS
Azide and BSA Free	NBP2-80635

compare

(select up to 3 products from this page to compare)

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Marker

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Kit Type

Lysate Type

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

PD-L1 Antibody - BSA Free

CD3 Antibody (SP7)

TIM-1/KIM-1/HAVCR Antibody - BSA Free

NFATC2/NFAT1 Antibody (25A10.D6.D2) - BSA Free

CD19 Antibody (CB19) - BSA Free

CD3 Antibody (OKT3) - BSA Free

PD-L1 Antibody (MIH5) - BSA Free

CD19 Antibody (1D3) - Azide and BSA Free

CD3 epsilon Antibody (C3e/1308)

PD-L1 Antibody - BSA Free

SH2D1A Antibody (1C9) - Azide and BSA Free

CD2 Antibody (RPA-2.10) - BSA Free