| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| HRP | NBP1-97358 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35812-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59941-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20789 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87949-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-46817-25ul | |||
![Western Blot: His Tag Antibody [NBP1-25939] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used for WB at 0.2 ug/ml (1:5,000). Detection: Chemiluminescence withan exposure time of 3 minutes.](http://images.novusbio.com/fullsize/His-Tag-Antibody-Western-Blot-NBP1-25939-img0001.jpg "Western Blot: His Tag Antibody [NBP1-25939] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used for WB at 0.2 ug/ml (1:5,000). Detection: Chemiluminescence withan exposure time of 3 minutes.")
![Western Blot: Rabbit IgG Isotype Control [NBP1-97041] - Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule (NBP2-36463). Lane 2: Rabbit IgG F(ab) Fragment (NBP1-97016). Lane 3: Rabbit IgG F(c) Fragment (NBP1-96784). Lane 4: Rabbit IgM Whole Molecule (NBP1-96968). Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Blocked in blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins. (NBP1-72732) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.](http://images.novusbio.com/fullsize/Rabbit-IgG-Isotype-Control-Western-Blot-NBP1-97041-img0006.jpg "Western Blot: Rabbit IgG Isotype Control [NBP1-97041] - Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule (NBP2-36463). Lane 2: Rabbit IgG F(ab) Fragment (NBP1-97016). Lane 3: Rabbit IgG F(c) Fragment (NBP1-96784). Lane 4: Rabbit IgM Whole Molecule (NBP1-96968). Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Blocked in blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins. (NBP1-72732) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.")
![Western Blot: Rabbit IgG Isotype Control [NBP1-97041]](http://images.novusbio.com/fullsize/Rabbit-IgG-Isotype-Control-Western-Blot-NBP1-97041-img0002.jpg)
![SDS-Page: Rabbit IgG Isotype Control [NBP1-97041] - Lane 1: Rabbit IgG - Non-Reduced. Lane 2: Rabbit IgG - Reduced. Load: 1.0 ug per lane. Predicted/Observed Size: Non-reduced - 130 kDa, Reduced - 55 and 28 kDa for Rabbit IgG.](http://images.novusbio.com/fullsize/Rabbit-IgG-Isotype-Control-SDS-Page-NBP1-97041-img0001.jpg)
![SDS-Page: Rabbit IgG Isotype Control [NBP1-97041] - SDS-PAGE of Rabbit IgG Isotype control. Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rabbit IgG Isotype control. Lane 2: Reduced Rabbit IgG F(ab) Fragment. Lane 3: Reduced Rabbit IgG F(c) Fragment. Lane 4: Reduced Rabbit IgM Whole Molecule. Load: 1 ug for F(ab) and F(c); 1.2 ug for IgG and IgM. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.](http://images.novusbio.com/fullsize/Rabbit IgG Isotype Control-SDS-Page-NBP1-97041-img0008.jpg)
![SDS-Page: Rabbit IgG Isotype Control [NBP1-97041] - SDS-PAGE of Rabbit IgG Isotype control. Lane 1: Non-reduced Rabbit IgG Isotype control. Lane 2: 5uL OPAL Pre-stained Marker. Lane 3: Reduced Rabbit IgG Isotype control. Load: 1ug per lane. Predicted/Observed size: Non-reduced at 150-170 kDa , Reduced at 55, 25 kDa.](http://images.novusbio.com/fullsize/Rabbit-IgG-Isotype-Control-SDS-Page-NBP1-97041-img0007.jpg)
![Western Blot: ND OLLAS/DYKDDDDK Vector [NBA1-07084] - Western blot comparison of binding sensitivity of Novus Biologicals' monoclonal antibodies anti-OLLAS NBP1-06713, anti-DYKDDDDK NBP1-06712 and the FLAG-M2* monoclonal antibody from Sigma-Aldrich. *FLAG TM and ANTI-FLAG TM are registered trademarks of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.](http://images.novusbio.com/fullsize/ND-OLLAS-DYKDDDDK-Vector-Western-Blot-NBA1-07084-img0003.jpg "Western Blot: ND OLLAS/DYKDDDDK Vector [NBA1-07084] - Western blot comparison of binding sensitivity of Novus Biologicals' monoclonal antibodies anti-OLLAS NBP1-06713, anti-DYKDDDDK NBP1-06712 and the FLAG-M2* monoclonal antibody from Sigma-Aldrich. *FLAG TM and ANTI-FLAG TM are registered trademarks of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.")
![ND OLLAS/DYKDDDDK Vector [NBA1-07084] - NBA1-07084: Vector Map of pCMV-ND OLLAS](http://images.novusbio.com/fullsize/ND-OLLAS-DYKDDDDK-Vector-NBA1-07084-img0004.jpg)
![Western Blot: HA Tag Antibody [NBP1-70006] - Detection of 1.0 ug of 12-Epitope Tag Protein Marker Lysate containing the HA epitope tag. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The lysate was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with anti-HA tag antibody for 1 h at room temperature followed by washes and reaction with a 1:20,000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 for 30 min at room temperature.](http://images.novusbio.com/fullsize/HA-Epitope-Tag-Antibody-Western-Blot-NBP1-70006-img0004.jpg "Western Blot: HA Tag Antibody [NBP1-70006] - Detection of 1.0 ug of 12-Epitope Tag Protein Marker Lysate containing the HA epitope tag. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The lysate was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with anti-HA tag antibody for 1 h at room temperature followed by washes and reaction with a 1:20,000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 for 30 min at room temperature.")
![Immunohistochemistry-Paraffin: HA Tag Antibody [NBP1-70006] - Arrowheads point to expression of HA tagged proteins in endothelial cells of mouse aorta. Sections of 4 um were prepared from representative paraffin blocks. Sections were then deparaffinized and rehydrated with xylene and alcohol. Citrate buffer antigen retrieval was performed for 30 min in a boiling jar. Anti-HA was diluted in blocking buffer at 1:2,000 and reacted at 4C overnight followed by signal detection using horseradish peroxidase with DAB as the chromogenic substrate. Tissue was counterstained with Mayer's hematoxylin. Personal Communication, Behzad Yeganeh,U. Manitoba, Winnipeg, Canada.](http://images.novusbio.com/fullsize/HA-Epitope-Tag-Antibody-Immunohistochemistry-Paraffin-NBP1-70006-img0001.jpg)
![Western Blot: Maltose Binding Protein Antibody - BSA Free [NBP1-97370] - Western Blot of Maltose Binding protein antibody - BSA Free.Lane 1: 1.0 ug of recombinant protein containing the Maltose Binding Protein.Primary Antibody: Polyclonal rabbit-anti-Maltose Binding Protein at 0.5-1.0 ug/ml for 1 h at room temperature.Secondary Antibody: 1:2500 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] for 30 min at room temperature. Imaging: LICOR's Odyssey(R) Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results. Predicted MW: ~42 kDa. A minor band at corresponding to multimers of this protein is also evident.](http://images.novusbio.com/fullsize/Maltose-Binding-Protein-Antibody---BSA-Free-Western-Blot-NBP1-97370-img0006.jpg "Western Blot: Maltose Binding Protein Antibody - BSA Free [NBP1-97370] - Western Blot of Maltose Binding protein antibody - BSA Free.Lane 1: 1.0 ug of recombinant protein containing the Maltose Binding Protein.Primary Antibody: Polyclonal rabbit-anti-Maltose Binding Protein at 0.5-1.0 ug/ml for 1 h at room temperature.Secondary Antibody: 1:2500 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] for 30 min at room temperature. Imaging: LICOR's Odyssey(R) Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results. Predicted MW: ~42 kDa. A minor band at corresponding to multimers of this protein is also evident.")
![Western Blot: Maltose Binding Protein Antibody - BSA Free [NBP1-97370] - Western Blot showing detection of Maltose Binding protein antibody - BSA Free.Lane 1: MW markers. Lane 2: Maltose Binding Protein [0.05 ug]. Protein was run on a 4-20% gel and transferred to 0.45 um nitrocellulose. Blocking with 1% BSA-TTBS 30 min at 20C. Primary Antibody: Anti-MBP (RABBIT) antibody was used at 1:1000 overnight at 4C. Secondary Antibody: Anti-Rabbit IgG (GOAT) IRDye800(R) conjugated antibody was used at 1:20,000 in Blocking Buffer for Fluorescent Western Blotting for 30 min at 20C. Imaged on the LiCor Odyssey imaging system. Predicted MW: ~42 kDa, the other bands present are recombinant MBP breakdown.](http://images.novusbio.com/fullsize/Maltose-Binding-Protein-Antibody---BSA-Free-Western-Blot-NBP1-97370-img0005.jpg)
![N/A: Maltose Binding Protein Antibody - BSA Free [NBP1-97370]](http://images.novusbio.com/fullsize/Maltose-Binding-Protein-Antibody---BSA-Free-N-A-NBP1-97370-img0004.jpg)
![Anti-MBP epitope tag polyclonal antibody detects MBP-tagged recombinant proteins by western blot. Polyclonal rabbit-anti-MBP epitope tag at 0.5-1.0 ug/ml was used to detect 1.0 ug of recombinant protein containing the MBP epitope tag. The apparent molecular weight of this band is 42 kDa. A minor band at corresponding to multimers of this protein is also evident. A 4-20% gradient gel was used to separate the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] (code ) for 30 min at room temperature. LICOR's Odyssey(R) Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.](http://images.novusbio.com/fullsize/nbp1-97370_rabbit-polyclonal-maltose-binding-protein-antibody-255202313135159.jpg)
![Western Blot: c-Myc Overexpression Lysate (Adult Normal) [NBL1-13414] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for c-Myc.](http://images.novusbio.com/fullsize/c-Myc-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13414-img0002.jpg "Western Blot: c-Myc Overexpression Lysate (Adult Normal) [NBL1-13414] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for c-Myc.")
![Immunocytochemistry/ Immunofluorescence: GSTA1 Antibody [NBP3-35812] - Immunofluorescence analysis of C6 cells using GSTA1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35812_rabbit-gsta1-pab-912202415111840.jpg "Immunocytochemistry/ Immunofluorescence: GSTA1 Antibody [NBP3-35812] - Immunofluorescence analysis of C6 cells using GSTA1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunocytochemistry/ Immunofluorescence: GSTA1 Antibody [NBP3-35812] - Immunofluorescence analysis of L929 cells using GSTA1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35812_rabbit-gsta1-pab-912202414213747.jpg)
![Western Blot: GSTA1 Antibody [NBP3-35812] - Western blot analysis of various lysates using GSTA1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-35812_rabbit-gsta1-pab-91220241418113.jpg)
![Immunocytochemistry/ Immunofluorescence: GSTA1 Antibody [NBP3-35812] - Immunofluorescence analysis of U-2 OS cells using GSTA1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35812_rabbit-gsta1-pab-912202415121348.jpg)
![Western Blot: A. aeolicus BPL/BioID2 Antibody (SS 3A5-E2) [NBP2-59941] - Analysis in total cell lysate of parental HeLa cell line (lane 1) or HeLa polyclonal cell line stably expressing BioID2-TorsinA delta E302/3 without (lane 2) or with induced expression (lane 3) were transferred to a nitrocellulose membrane and blotted for BPL R40G/BioID2 with SS 3A5-E2 antibody. The predicted molecular weight of BioID2-Torsin A Delta E302/3 fusion protein is 65 kDa](http://images.novusbio.com/fullsize/A-aeolicus-BPL-BioID2-Antibody-SS-3A5-E2-Western-Blot-NBP2-59941-img0007.jpg "Western Blot: A. aeolicus BPL/BioID2 Antibody (SS 3A5-E2) [NBP2-59941] - Analysis in total cell lysate of parental HeLa cell line (lane 1) or HeLa polyclonal cell line stably expressing BioID2-TorsinA delta E302/3 without (lane 2) or with induced expression (lane 3) were transferred to a nitrocellulose membrane and blotted for BPL R40G/BioID2 with SS 3A5-E2 antibody. The predicted molecular weight of BioID2-Torsin A Delta E302/3 fusion protein is 65 kDa")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: A. aeolicus BPL/BioID2 Antibody (SS 3A5-E2) [NBP2-59941] - A) Parental HEK293T or (B) monoclonal HEK293T cell line with BioID2 knocked-in into at least one LMNA gene alleles (resulting in BioID2 Lamin A/C fusion protein) were immunofluorescently labelled with anti-BPL R40G/BioID2 SS 3A5-E2 monoclonal antibody (green). The nuclei were visualized with Hoechst.](http://images.novusbio.com/fullsize/A-aeolicus-BPL-BioID2-Antibody-SS-3A5-E2-Immunocytochemistry-Immunofluorescence-NBP2-59941-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: A. aeolicus BPL/BioID2 Antibody (SS 3A5-E2) [NBP2-59941] - Staining of HeLa polyclonal cell line expressing BioID2 tagged to TorsinA delta E302/3 with SS 3A5-E2 monoclonal antibody. Cells were fixed in (A) cold methanol or (B) 4% PFA and stained with SS anti-BPL R40G/BioID2 3A5-E2 antibody (in green). The nuclei were counter-stained with Hoechst.](http://images.novusbio.com/fullsize/A-aeolicus-BPL-BioID2-Antibody-SS-3A5-E2-Immunocytochemistry-Immunofluorescence-NBP2-59941-img0002.jpg)
![SDS-Page: Recombinant Human Cystatin C Protein [NBP2-61381]](http://images.novusbio.com/fullsize/Recombinant-Human-Cystatin-C-Protein-SDS-Page-NBP2-61381-img0002.jpg "SDS-Page: Recombinant Human Cystatin C Protein [NBP2-61381]")
![Immunohistochemistry-Paraffin: Enteropeptidase/Enterokinase Antibody [NBP1-87949] - Staining of human colon shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/Enteropeptidase-Enterokinase-Antibody-Immunohistochemistry-Paraffin-NBP1-87949-img0006.jpg "Immunohistochemistry-Paraffin: Enteropeptidase/Enterokinase Antibody [NBP1-87949] - Staining of human colon shows no positivity in glandular cells as expected.")
![Immunohistochemistry-Paraffin: Enteropeptidase/Enterokinase Antibody [NBP1-87949] - Staining of human duodenum shows moderate membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Enteropeptidase-Enterokinase-Antibody-Immunohistochemistry-Paraffin-NBP1-87949-img0005.jpg)
![Western Blot: GSTA1 Antibody [NBP2-46817] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10, Lane 2: Human cell line RT-4, Lane 3: Human cell line U-251 MG, Lane 4: Human plasma, Lane 5: Human Analysis of human liver tissue.](http://images.novusbio.com/fullsize/GSTA1-Antibody-Western-Blot-NBP2-46817-img0003.jpg "Western Blot: GSTA1 Antibody [NBP2-46817] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10, Lane 2: Human cell line RT-4, Lane 3: Human cell line U-251 MG, Lane 4: Human plasma, Lane 5: Human Analysis of human liver tissue.")
![Immunocytochemistry/Immunofluorescence: GSTA1 Antibody [NBP2-46817] - Staining of human cell line Hep G2 shows localization to cytosol.](http://images.novusbio.com/fullsize/GSTA1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-46817-img0004.jpg)
![Immunohistochemistry: GSTA1 Antibody [NBP2-46817] - Staining of human Analysis of human liver tissue. shows strong cytoplasmic and nuclear positivity in hepatocytes.](http://images.novusbio.com/fullsize/GSTA1-Antibody-Immunohistochemistry-NBP2-46817-img0001.jpg)