ND OLLAS/DYKDDDDK Epitope Tag Epitope Tag Kit

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Western Blot: ND OLLAS/DYKDDDDK Epitope Tag Kit [NBK1-19033] - Comparison of binding sensitivity of Novus Biologicals' monoclonal antibodies OLLAS NBP1-06713, anti-DYKDDDDK NBP1-06712 and the FLAG-M2 monoclonal antibody ...read more
ND OLLAS/DYKDDDDK Epitope Tag Kit [NBK1-19033] - Vector Map of pCMV-SD OLLAS

Product Details

Summary
Reactivity NASpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IP

Order Details

ND OLLAS/DYKDDDDK Epitope Tag Epitope Tag Kit Summary

Kit Type
Epitope Tag Kit

Applications/Dilutions

Application Notes
This kit is useful for immunofluorescence, immunoprecipitation, Western blot, immunocytochemistry, and immunohistochemistry-frozen sections.

Reactivity Notes

Tag specific

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.

Kit Components

Alternate Names for ND OLLAS/DYKDDDDK Epitope Tag Epitope Tag Kit

  • OLLA-2

Background

The pCMV-N-DYK-OLLAS vector provides a convenient vector to express recombinant proteins under control of the CMV promoter with both an N-terminal OLLAS tag and a DYKDDDDK tag. Using this vector the protein will be expressed in the cytoplasm. We recommend inserting your protein of interest into the vector in frame using the Xba I and EcoR I/Not I restriction sites shown above. Bacterial selection can be performed using Kanamycin, while Neomycin resistance can be used for screening mammalian transfectants. The OLLAS tag has been shown to be detectable at ~100-fold lower levels that similar commercially available tags using the OLLA-2 monoclonal antibody. The OLLAS tag has also been demonstrated to provide superior functionality whether used as an N-terminal, C-terminal, or internal tag on recombinant and fusion proteins without interfering with the biological activity of the tagged protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Additional ND OLLAS/DYKDDDDK Epitope Tag Products

ND OLLAS/DYKDDDDK Epitope Tag NBK1-19033

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