ND OLLAS/DYKDDDDK Epitope Tag Epitope Tag Kit


Western Blot: ND OLLAS/DYKDDDDK Epitope Tag Kit [NBK1-19033] - Comparison of binding sensitivity of Novus Biologicals' monoclonal antibodies OLLAS NBP1-06713, anti-DYKDDDDK NBP1-06712 and the FLAG-M2 monoclonal antibody ...read more
ND OLLAS/DYKDDDDK Epitope Tag Kit [NBK1-19033] - Vector Map of pCMV-SD OLLAS

Product Details

Reactivity NASpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IP
Concentration is not relevant for this product. Please see the protocols for proper use of this product.

Order Details

ND OLLAS/DYKDDDDK Epitope Tag Epitope Tag Kit Summary

Kit Type
Epitope Tag Kit

Packaging, Storage & Formulations

Store at 4C. Do not freeze.


  • Western Blot
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunoprecipitation
Application Notes
This kit is useful for immunofluorescence, immunoprecipitation, Western blot, immunocytochemistry, and immunohistochemistry-frozen sections.

Kit Components

Reactivity Notes

Tag specific

Alternate Names for ND OLLAS/DYKDDDDK Epitope Tag Epitope Tag Kit

  • OLLA-2


The pCMV-N-DYK-OLLAS vector provides a convenient vector to express recombinant proteins under control of the CMV promoter with both an N-terminal OLLAS tag and a DYKDDDDK tag. Using this vector the protein will be expressed in the cytoplasm. We recommend inserting your protein of interest into the vector in frame using the Xba I and EcoR I/Not I restriction sites shown above. Bacterial selection can be performed using Kanamycin, while Neomycin resistance can be used for screening mammalian transfectants. The OLLAS tag has been shown to be detectable at ~100-fold lower levels that similar commercially available tags using the OLLA-2 monoclonal antibody. The OLLAS tag has also been demonstrated to provide superior functionality whether used as an N-terminal, C-terminal, or internal tag on recombinant and fusion proteins without interfering with the biological activity of the tagged protein.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for ND OLLAS/DYKDDDDK Epitope Tag Kit (NBK1-19033) (0)

There are no publications for ND OLLAS/DYKDDDDK Epitope Tag Kit (NBK1-19033).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for ND OLLAS/DYKDDDDK Epitope Tag Kit (NBK1-19033) (0)

There are no reviews for ND OLLAS/DYKDDDDK Epitope Tag Kit (NBK1-19033). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ND OLLAS/DYKDDDDK Epitope Tag Kit (NBK1-19033) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional ND OLLAS/DYKDDDDK Epitope Tag Products

ND OLLAS/DYKDDDDK Epitope Tag NBK1-19033

Research Areas for ND OLLAS/DYKDDDDK Epitope Tag Kit (NBK1-19033)

Find related products by research area.

Blogs on ND OLLAS/DYKDDDDK Epitope Tag

There are no specific blogs for ND OLLAS/DYKDDDDK Epitope Tag, but you can read our latest blog posts.

Contact Information

Product PDFs

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NBK1-19033 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.