Biotin Antibody [PE]

• Reactivity: All-NA
• Applications: WB, ELISA, Flow, ICC/IF, KO
• Clonality: Polyclonal
• Host: Goat
• Conjugate: PE
• Concentration: LYOPH

Biotin Antibody [PE] Summary

• Description: Store vial at 4C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
  
  Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA)-Immunoglobulin and Protease free
  Color: Red Excitation Wavelength: 488 Emission Wavelength: 575
  Conjugation Chemistry: SATA/MBS
  
  This antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Phycoerythrin, Biotin conjugated IgG and Biotin conjugated Bovine Serum Albumin.
• Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (b-KLH)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:50-1:200
  • Flow Cytometry 1.0 ug/1000000 cells
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Knockout Validated
  • Western Blot 1:50-1:200
• Application Notes: This product detects biotin and biocytin by ELISA, Immuno­histo­chemistry (IHC), and the antibody also recognizes biotin conjugated to various immunoglobulins. The antibody may be used in amplification techniques, immunoblotting, in situ nucleic acid hybridization, flow cytometry or Immunofluorescence microscopy.

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at 4C in the dark. Do not freeze.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
• Preservative: 0.05% Sodium Azide
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute with 1.0 ml deionized water (or equivalent)

Alternate Names for Biotin Antibody [PE]

• Biotin
• Vitamin B4

Background

Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.