AU5 Epitope Tag Antibody


Western Blot: AU5 Epitope Tag Antibody [NB600-461] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.2 ug/ml (1:5,000).

Product Details

Reactivity NASpecies Glossary
Applications WB, ELISA, ICC/IF, IP
1.0 mg/ml

Order Details

AU5 Epitope Tag Antibody Summary

Goats were immunized with AU5 (TDFYLK) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.
Immunogen affinity purified
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  • Western Blot 1:1000-1:10000
  • ELISA Primary-1:1000-1:30000; Coating: 1:100-1:500
  • Immunocytochemistry/Immunofluorescence 1:100-1:400
  • Immunoprecipitation 1 - 4ug/mg lysate
Application Notes
ELISA: Coating: Use at a dilution of 1/100 - 1/500. Primary: Use at a dilution of 1/1,000 - 1/30,000. ICC: Use at a dilution of 1/100 - 1/400. WB: Colorimetric: Use at a dilution of 1/1,000 - 1/10,000. Chemiluminescent: Use at a dilution of 1/1,000 - 1/30,000.

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
0.09% Sodium Azide
1.0 mg/ml
Immunogen affinity purified

Alternate Names for AU5 Epitope Tag Antibody

  • AU5 epitope tag
  • TDFYLK epitope tag
  • TDFYLK tag


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for AU5 Epitope Tag Antibody (NB600-461). (Showing 1 - 1 of 1 FAQ).

  1. Do you have any kinetic data on the antibody affinity (i.e. Kd, Kon, Koff)?
    • The determination of Kd, on-rates, and off-rates are only possible with pure protein preparations where concentration and amounts are known and controlled. This is possible when measuring monoclonal antibody affinity to its target protein. However, polyclonal populations, such as this product, are not uniform and contain a mixture. Unfortunately, measuring the Kd of such a mixture is not possible.

Secondary Antibodies


Isotype Controls

Additional AU5 Epitope Tag Antibody Products

AU5 Epitope Tag NB600-461

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