AU5 Epitope Tag Antibody

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Western Blot: AU5 Epitope Tag Antibody [NB600-461] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.2 ug/ml (1:5,000).

Product Details

Summary
Reactivity NASpecies Glossary
Applications WB, ELISA, ICC/IF, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

AU5 Epitope Tag Antibody Summary

Immunogen
Goats were immunized with AU5 (TDFYLK) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • ELISA Primary-1:1000-1:30000; Coating: 1:100-1:500
  • Immunocytochemistry/Immunofluorescence 1:100-1:400
  • Immunoprecipitation 1 - 4ug/mg lysate
Application Notes
ELISA: Coating: Use at a dilution of 1/100 - 1/500. Primary: Use at a dilution of 1/1,000 - 1/30,000. ICC: Use at a dilution of 1/100 - 1/400. WB: Colorimetric: Use at a dilution of 1/1,000 - 1/10,000. Chemiluminescent: Use at a dilution of 1/1,000 - 1/30,000.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for AU5 Epitope Tag Antibody

  • AU5 epitope tag
  • TDFYLK epitope tag
  • TDFYLK tag

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for AU5 Epitope Tag Antibody (NB600-461). (Showing 1 - 1 of 1 FAQ).

  1. Do you have any kinetic data on the antibody affinity (i.e. Kd, Kon, Koff)?
    • The determination of Kd, on-rates, and off-rates are only possible with pure protein preparations where concentration and amounts are known and controlled. This is possible when measuring monoclonal antibody affinity to its target protein. However, polyclonal populations, such as this product, are not uniform and contain a mixture. Unfortunately, measuring the Kd of such a mixture is not possible.

Secondary Antibodies

 

Isotype Controls

Additional AU5 Epitope Tag Products

AU5 Epitope Tag NB600-461

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