SD OLLAS/DYKDDDDK Epitope Tag Kit Summary
Packaging, Storage & Formulations
Store at 4C. Do not freeze.
Concentration is not relevant for this product. Please see the protocols for proper use of this product.
This kit is useful for immunofluorescence, immunoprecipitation, Western blot, immunocytochemistry, and immunohistochemistry-frozen sections.
Alternate Names for SD OLLAS/DYKDDDDK Epitope Tag Kit
The pCMV-NS-OLLAS vector provides a convenient vector to express recombinant proteins under control of the CMV promoter with both an N-terminal OLLAS tag and a DYKDDDDK tag. Using this vector the protein will be secreted from the cell due to the inclusion of a signal peptide. We recommend inserting your protein of interest into the vector in frame using the Xba I and EcoR I/Not I restriction sites shown above. Bacterial selection can be performed using Kanamycin, while Neomycin resistance can be used for screening mammalian transfectants. The OLLAS tag has been shown to be detectable at ~100-fold lower levels that similar commercially available tags using the OLLA-2 monoclonal antibody. The OLLAS tag has also been demonstrated to provide superior functionality whether used as an N-terminal, C-terminal, or internal tag on recombinant and fusion proteins without interfering with the biological activity of the tagged protein.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed
for 6 months from date of receipt.
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