SD OLLAS/DYKDDDDK Expression and Detection Kit


Western Blot: SD OLLAS/DYKDDDDK Epitope Tag Kit [NBK1-19035] - Comparison of binding sensitivity of Novus Biologicals' monoclonal antibodies OLLAS NBP1-06713, anti-DYKDDDDK NBP1-06712 and the FLAG-M2 monoclonal antibody more
SD OLLAS/DYKDDDDK Epitope Tag Kit [NBK1-19035] - Vector Map of pCMV-SD OLLAS

Product Details

Reactivity All-NASpecies Glossary
Applications WB, ICC/IF, IHC, IP

Order Details

SD OLLAS/DYKDDDDK Expression and Detection Kit Summary

Kit Type
Expression and Detection Kit


  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunoprecipitation
  • Western Blot

Reactivity Notes

Tag specific

Packaging, Storage & Formulations

Store at 4C. Do not freeze.

Kit Components

Alternate Names for SD OLLAS/DYKDDDDK Expression and Detection Kit

  • OLLA-2


The pCMV-NS-OLLAS vector provides a convenient vector to express recombinant proteins under control of the CMV promoter with both an N-terminal OLLAS tag and a DYKDDDDK tag. Using this vector the protein will be secreted from the cell due to the inclusion of a signal peptide. We recommend inserting your protein of interest into the vector in frame using the Xba I and EcoR I/Not I restriction sites shown above. Bacterial selection can be performed using Kanamycin, while Neomycin resistance can be used for screening mammalian transfectants. The OLLAS tag has been shown to be detectable at ~100-fold lower levels that similar commercially available tags using the OLLA-2 monoclonal antibody. The OLLAS tag has also been demonstrated to provide superior functionality whether used as an N-terminal, C-terminal, or internal tag on recombinant and fusion proteins without interfering with the biological activity of the tagged protein.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Additional SD OLLAS/DYKDDDDK Products

Array NBK1-19035

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