Biotin Antibody [Alkaline Phosphatase]

• Reactivity: All-NA
• Applications: WB, ELISA, IHC, KO
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alkaline Phosphatase

Biotin Antibody [Alkaline Phosphatase] Summary

• Description: Store vial at 4C before opening. DO NOT FREEZE. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
  
  This product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum and Biotinylated IgG.
• Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (b-KLH)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:3000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Knockout Validated
  • Western Blot 1:500-1:2500
• Application Notes: This product has been tested by ELISA an is suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
• Preservative: 0.1% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Biotin Antibody [Alkaline Phosphatase]

• Biotin
• Vitamin B4

Background

Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Biotin Antibody (NB120-6652). (Showing 1 - 1 of 1 FAQ).

1. We have a customer who is interested in NB120-6652 but is in need of an unconjugated product. Do you by chance sell this same Ab in the unconjugated form?
   • We don't have this specific one in the unconjugated form however, we do have other antibodies against Biotin that are unconjugated and may work for your customer.