![Immunocytochemistry/Immunofluorescence: Strep II Tag Antibody (661) [NBP2-43719] - Analysis of Strep/GFP-tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Red: Strep-recombinant protein stained by Strep-tag antibody diluted at 1:500. Green: GFP is expressed in the tranfected cell. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Strep-II-Tag-Antibody-661-Immunocytochemistry-Immunofluorescence-NBP2-43719-img0001.jpg "Immunocytochemistry/Immunofluorescence: Strep II Tag Antibody (661) [NBP2-43719] - Analysis of Strep/GFP-tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Red: Strep-recombinant protein stained by Strep-tag antibody diluted at 1:500. Green: GFP is expressed in the tranfected cell. Blue: Hoechst 33342 staining.")
| Reactivity | ETSpecies Glossary |
| Applications | WB, ICC/IF, IP |
| Clone | 661 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Immunogen | Immunogen Amino acid sequence - ASWSHPQFEKGA |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS, 20% Glycerol |
| Preservative | No Preservative |
| Purity | Protein G purified |
| Publication using NBP2-43719 | Applications | Species |
|---|---|---|
| Yun HYH Molecular Characterization of Oncogenic H3. 3 K27M and G34R Histone Mutants Thesis 2021-01-01 |
Secondary Antibodies |
Isotype Controls |
Research Areas for Strep II Tag Antibody (NBP2-43719)Find related products by research area.
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![Immunoprecipitation: Strep II Tag Antibody (661) [NBP2-43719] - Analysis of 293T whole cell lysate/extractA : 20 ug whole cell lysate/extract of strep tagged protein expressing 293T cellsB : Control with 2.5 ug of pre-immune mouse IgGC : Immunoprecipitation of strep tagged protein by 2.5 ug of strep tag antibody 10% SDS-PAGE The immunoprecipitated strep tagged protein was detected by strep tag antibody diluted at 1 : 3000. EasyBlot anti-mouse IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/Strep-II-Tag-Antibody-661-Immunoprecipitation-NBP2-43719-img0002.jpg "Immunoprecipitation: Strep II Tag Antibody (661) [NBP2-43719] - Analysis of 293T whole cell lysate/extractA : 20 ug whole cell lysate/extract of strep tagged protein expressing 293T cellsB : Control with 2.5 ug of pre-immune mouse IgGC : Immunoprecipitation of strep tagged protein by 2.5 ug of strep tag antibody 10% SDS-PAGE The immunoprecipitated strep tagged protein was detected by strep tag antibody diluted at 1 : 3000. EasyBlot anti-mouse IgG (HRP) was used as a secondary reagent.")
![Western Blot: Strep II Tag Antibody (661) [NBP2-43719] -Non-transfected (-) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Strep epitope Tag antibody [661] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp2-43719_mouse-monoclonal-strep-ii-tag-antibody-661-western-blot-273202482449..jpg "Western Blot: Strep II Tag Antibody (661) [NBP2-43719] -Non-transfected (-) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Strep epitope Tag antibody [661] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
