| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48089 | |||
| Azide and BSA Free | NBP2-86682 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| DyLight 800 | NBP3-11666 | |||
| FITC | NBP3-11665 | |||
| HRP | NBP3-11668 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-37823-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59941-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52703-0.025mg | |||
![Western Blot: N OLLAS Vector [NBA1-07083] - Western blot comparison of binding sensitivity of Novus Biologicals monoclonal antibodies OLLAS NBP1-06713, anti-DYKDDDDK NBP1-06712 and the FLAG-M2 monoclonal antibody from Sigma-Aldrich. FLAG TM and ANTI-FLAG TM are registered trademarks of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.](http://images.novusbio.com/fullsize/N-OLLAS-Vector-Western-Blot-NBA1-07083-img0002.jpg "Western Blot: N OLLAS Vector [NBA1-07083] - Western blot comparison of binding sensitivity of Novus Biologicals monoclonal antibodies OLLAS NBP1-06713, anti-DYKDDDDK NBP1-06712 and the FLAG-M2 monoclonal antibody from Sigma-Aldrich. FLAG TM and ANTI-FLAG TM are registered trademarks of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.")
![N OLLAS Vector [NBA1-07083] - Vector map of pCMV-N OLLAS](http://images.novusbio.com/fullsize/N-OLLAS-Vector-NBA1-07083-img0003.jpg)
![Western Blot: SD OLLAS/DYKDDDDK Vector [NBA1-07085] - Western blot comparison of binding sensitivity of Novus Biologicals monoclonal antibodies OLLAS NBP1-06713, anti-DYKDDDDK NBP1-06712 and the FLAG-M2 monoclonal antibody from Sigma-Aldrich. FLAG TM and ANTI-FLAG TM are registered trademarks of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.](http://images.novusbio.com/fullsize/SD-OLLAS-DYKDDDDK-Vector-Western-Blot-NBA1-07085-img0001.jpg "Western Blot: SD OLLAS/DYKDDDDK Vector [NBA1-07085] - Western blot comparison of binding sensitivity of Novus Biologicals monoclonal antibodies OLLAS NBP1-06713, anti-DYKDDDDK NBP1-06712 and the FLAG-M2 monoclonal antibody from Sigma-Aldrich. FLAG TM and ANTI-FLAG TM are registered trademarks of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.")
![SD OLLAS/DYKDDDDK Vector [NBA1-07085] - Vector Map of pCMV-SD OLLAS](http://images.novusbio.com/fullsize/SD-OLLAS-DYKDDDDK-Vector-NBA1-07085-img0002.jpg)
![Western Blot: His Tag Antibody (33D10.D2.G8) [NBP1-42781] - Analysis using Biotin conjugate of NBP1-42781. Lane 1: 100 ng Purified histidine-tagged recombinant protein. Lane 2: 200 ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100 ng Purified GST-tagged recombinant protein. Lane 4: 100 ng Purified FLAG-tagged recombinant protein. Primary antibody: Mouse anti-6xHIS Tag antibody at 1:5,000 overnight at 4C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20,000 for 30 min at RT. Block: 5% BLOTTO for 1 hr at RT.](http://images.novusbio.com/fullsize/His-Tag-Antibody-33D10-D2-G8-Western-Blot-NBP1-42781-img0020.jpg "Western Blot: His Tag Antibody (33D10.D2.G8) [NBP1-42781] - Analysis using Biotin conjugate of NBP1-42781. Lane 1: 100 ng Purified histidine-tagged recombinant protein. Lane 2: 200 ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100 ng Purified GST-tagged recombinant protein. Lane 4: 100 ng Purified FLAG-tagged recombinant protein. Primary antibody: Mouse anti-6xHIS Tag antibody at 1:5,000 overnight at 4C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20,000 for 30 min at RT. Block: 5% BLOTTO for 1 hr at RT.")
![ELISA: His Tag Antibody (33D10.D2.G8) [NBP1-42781] - ELISA of Mouse anti-6xHIS Tag Antibody. Antigen: HIS-tagged purified protein and E. coli cell lysates expressing HIS-Tagged construct, GST- and RON-tagged purified proteins. Coating amount: 0.15ug per well. Primary antibody: 6xHIS Tag antibody at 100 ug/mL. Dilution series: 2-fold. Mid-point concentration: 200 ng/mL. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000. Substrate: TMB.](http://images.novusbio.com/fullsize/His-Tag-Antibody-33D10-D2-G8-ELISA-NBP1-42781-img0021.jpg)
![Immunocytochemistry/ Immunofluorescence: Luciferase Antibody [NBP2-19295] - Firefly Luciferase antibody detects Firefly Luciferase protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Firefly Luciferase stained by Firefly Luciferase antibody diluted at 1:500.Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/antibody/nbp2-19295_rabbit-polyclonal-luciferase-antibody-immunocytochemistry-immunofluorescence-298202414621..jpg "Immunocytochemistry/ Immunofluorescence: Luciferase Antibody [NBP2-19295] - Firefly Luciferase antibody detects Firefly Luciferase protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Firefly Luciferase stained by Firefly Luciferase antibody diluted at 1:500.Blue: Fluoroshield with DAPI.")
![Western Blot: Luciferase Antibody [NBP2-19295] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Firefly Luciferase antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp2-19295_rabbit-polyclonal-luciferase-antibody-western-blot-298202414820..jpg)
![Immunoprecipitation: Strep II Tag Antibody (661) [NBP2-43719] - Analysis of 293T whole cell lysate/extractA : 20 ug whole cell lysate/extract of strep tagged protein expressing 293T cellsB : Control with 2.5 ug of pre-immune mouse IgGC : Immunoprecipitation of strep tagged protein by 2.5 ug of strep tag antibody 10% SDS-PAGE The immunoprecipitated strep tagged protein was detected by strep tag antibody diluted at 1 : 3000. EasyBlot anti-mouse IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/Strep-II-Tag-Antibody-661-Immunoprecipitation-NBP2-43719-img0002.jpg "Immunoprecipitation: Strep II Tag Antibody (661) [NBP2-43719] - Analysis of 293T whole cell lysate/extractA : 20 ug whole cell lysate/extract of strep tagged protein expressing 293T cellsB : Control with 2.5 ug of pre-immune mouse IgGC : Immunoprecipitation of strep tagged protein by 2.5 ug of strep tag antibody 10% SDS-PAGE The immunoprecipitated strep tagged protein was detected by strep tag antibody diluted at 1 : 3000. EasyBlot anti-mouse IgG (HRP) was used as a secondary reagent.")
![Strep epitope Tag antibody [GT661] detects Strep-recombinant protein by immunofluorescent analysis. Sample: Strep/GFP-tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Red: Strep-recombinant protein stained by Strep epitope Tag antibody [GT661] diluted at 1:500. Green: GFP is expressed in the tranfected cell. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-43719_mouse-monoclonal-strep-ii-tag-antibody-661-immunocytochemistry-immunofluorescence-261120251983610.jpg)
![Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Strep epitope Tag antibody [GT661] (NBP2-43719) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43719_mouse-monoclonal-strep-ii-tag-antibody-661-western-blot-2611202518213324.jpg)
![Immunohistochemistry-Paraffin: c-Myc Antibody (SPM237) [NBP2-45145] - Human Cervical Carcinoma stained with c-myc Monoclonal Antibody (SPM237).](http://images.novusbio.com/fullsize/c-Myc-Antibody-SPM237-Immunohistochemistry-Paraffin-NBP2-45145-img0001.jpg "Immunohistochemistry-Paraffin: c-Myc Antibody (SPM237) [NBP2-45145] - Human Cervical Carcinoma stained with c-myc Monoclonal Antibody (SPM237).")
![Western Blot: Mouse Pure-Blot anti-Rabbit IgG (H+L) Secondary Antibody (eB182) [DyLight 680] [NBP3-11667] - Lane 1: Rabbit IgG, Non-denatured. Lane 2: Rabbit IgG, Denatured. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Mouse Pure-Blot anti-Rabbit IgG (H+L) Secondary antibody (eB182) [DyLight 680] at 1:1,000 for 60 min at RT. Block for 30 min at RT. Predicted: 160 kDa for non-denatured; observed: 170-180 kDa for non-denatured. Band migrates at slightly higher molecular weight.](http://images.novusbio.com/fullsize/Mouse-Pure-Blot-anti-Rabbit-IgG-H+L-Secondary-Antibody-eB182-DyLight-680-Western-Blot-NBP3-11667-img0002.jpg "Western Blot: Mouse Pure-Blot anti-Rabbit IgG (H+L) Secondary Antibody (eB182) [DyLight 680] [NBP3-11667] - Lane 1: Rabbit IgG, Non-denatured. Lane 2: Rabbit IgG, Denatured. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Mouse Pure-Blot anti-Rabbit IgG (H+L) Secondary antibody (eB182) [DyLight 680] at 1:1,000 for 60 min at RT. Block for 30 min at RT. Predicted: 160 kDa for non-denatured; observed: 170-180 kDa for non-denatured. Band migrates at slightly higher molecular weight.")
![Western Blot: Mouse Pure-Blot anti-Rabbit IgG (H+L) Secondary Antibody (eB182) [DyLight 680] [NBP3-11667] - Jurkat cell lysate (0.5 ml of 1x10e7 cells/ml) was incubated with rabbit anti-human Stat1 and immunoprecipitated using Protein G, Protein A and Anti-Rabbit Ig IP Beads. Precipitate from 5x10e5 cells was subjected to electrophoresis, transferred to a PVDF membrane, and Western blotted with anti-Stat1 using Mouse Pure-Blot anti-Rabbit IgG (H+L) Secondary antibody (eB182) [DyLight 680].](http://images.novusbio.com/fullsize/Mouse-Pure-Blot-anti-Rabbit-IgG-H+L-Secondary-Antibody-eB182-DyLight-680-Western-Blot-NBP3-11667-img0003.jpg)
![Western Blot of Fluorescent TrueBlot(R): Anti-Rabbit IgG DyLight 680 Conjugated. Lane 1: Rabbit IgG, Non-denatured. Lane 2: Rabbit IgG, Denatured. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Mouse Pure-Blot anti-Rabbit IgG (H+L) Secondary Antibody (eB182) [DyLight 680]antibody at 1:1,000 for 60 min at RT. Block for 30 min at RT. Predicted: 160 kDa for non-denatured; observed: 170-180 kDa for non-denatured. Band migrates at slightly higher molecular weight.](http://images.novusbio.com/fullsize/nbp3-11667_mouse-monoclonal-mouse-pure-blot-anti-rabbit-igg-h-l-secondary-antibody-eb182-dylight-680-245202310564640.jpg)
![Mouse Pure-Blot anti-Rabbit IgG (H+L) Secondary Antibody (eB182) [DyLight 680] / Western Blot: Jurkat cell lysate (0.5 ml of 1x10e7 cells/ml) was incubated with rabbit anti-human Stat1 and immunoprecipitated using Protein G, Protein A and Anti-Rabbit Ig IP Beads. Precipitate from 5x10e5 cells was subjected to electrophoresis, transferred to a PVDF membrane, and Western blotted with anti-Stat1 using Rabbit TrueBlot(R): Anti-Rabbit IgG HRP](http://images.novusbio.com/fullsize/nbp3-11667_mouse-monoclonal-mouse-pure-blot-anti-rabbit-igg-h-l-secondary-antibody-eb182-dylight-680-255202313135150.jpg)
![Immunoprecipitation: STAT6 Antibody [NBP3-14726] - Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from UACC-62 cells.Antibodies: Rabbit anti-STAT6 recombinant monoclonalantibody used for IP at 20ul/mg lysate. STAT6 was also immunoprecipitated by otherantibodies against different epitopes of STAT6. For blotting immunoprecipitatedSTAT6, was used at 1:1000. Chemiluminescencewith an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/STAT6-Antibody-Immunoprecipitation-NBP3-14726-img0001.jpg "Immunoprecipitation: STAT6 Antibody [NBP3-14726] - Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from UACC-62 cells.Antibodies: Rabbit anti-STAT6 recombinant monoclonalantibody used for IP at 20ul/mg lysate. STAT6 was also immunoprecipitated by otherantibodies against different epitopes of STAT6. For blotting immunoprecipitatedSTAT6, was used at 1:1000. Chemiluminescencewith an exposure time of 30 seconds.")
![Western Blot: DYKDDDDK Epitope Tag Antibody (FG4R) [NBP2-37823] - Analysis using [NBP2-37823] at a 1:1000 (1ug/mL) dilution, probed against HEK293 cells transfected with DYKDDDDK-tagged protein vector; untransfected (1) and transfected (2). Observed molecular weight ~130 kDa.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-FG4R-Western-Blot-NBP2-37823-img0011.jpg "Western Blot: DYKDDDDK Epitope Tag Antibody (FG4R) [NBP2-37823] - Analysis using [NBP2-37823] at a 1:1000 (1ug/mL) dilution, probed against HEK293 cells transfected with DYKDDDDK-tagged protein vector; untransfected (1) and transfected (2). Observed molecular weight ~130 kDa.")
![Immunocytochemistry/Immunofluorescence: DYKDDDDK Epitope Tag Antibody (FG4R) [NBP2-37823] - Analysis of Chlamydia trachomatis FLAG-tagged IncA (blue) in HeLa cells. Cells were infected with a GFP-expressing strain of Chlamydia trachomatis containing FLAG-IncA under the control of the Tetracycline (Tet) promoter, and either left untreated (0ng/ml Tet) or treated with anhydrotetracycline (10ng/ml Tet). Cells were fixed with 2% paraformaldehyde in PBS for 30 min at RT, permeabilized and blocked in 10% goat serum, 0.1% BSA, 0.1% saponin in PBS pH 7.4 for 30 mins at RT, then probed with a DYKDDDDK Epitope Tag Antibody (FG4R) [NBP2-37823] at 1:500 for 1 hr at RT. Cells were washed with PBS, and incubated with an AlexaFluor 647-conjugated goat anti-mouse IgG secondary antibody at 1:2000 for 1 hr at RT. Images were taken on a fluorescent microscope using a 60X oil-immersion objective. Scale bars represent 20um. Data courtesy of the Innovators Program.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-FG4R-Immunocytochemistry-Immunofluorescence-NBP2-37823-img0009.jpg)
![Western Blot: DYKDDDDK Epitope Tag Antibody (FG4R) [NBP2-37823] - Analysis of 30ug of whole cell lysates from HeLa cells infected with a strain of Chlamydia trachomatis expressing FLAG-IncA under the control of a Tetracycline promoter (Tet), and either left untreated (-) or treated with anhydrotetracycline (+), onto an SDS-PAGE gel, using [NBP2-37823]. Observed molecular weight ~30 kDa.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-FG4R-Western-Blot-NBP2-37823-img0012.jpg)
![<b>Biological Strategies Validation. </b>Immunoprecipitation: DYKDDDDK Epitope Tag Antibody (FG4R) [NBP2-37823] - Analysis of Chlamydia trachomatis FLAG-tagged IncA in HeLa cells infected with a strain of Chlamydia trachomatis expressing FLAG-IncA under control of a Tetracycline (Tet) promoter, untreated (-) or treated with anhydrotetracycline (+). Complexes formed by incubating 1ml of HeLa cell lysate with 3ug of [NBP2-37823] overnight at 4C. Immune complexes were captured on 20ul Protein G agarose and eluted by boiling in Laemmli sample buffer. Samples were resolved on a 10% Bis-Tris acrylamide gel, transferred to a PVDF membrane, and blocked with 5% milk in TBST for 1 hr at RT. Membrane was probed with a rabbit anti-FLAG pAb at 1:200 in 3% BSA in TBST overnight at 4C, washed in TBST, and incubated with an HRP-conjugated donkey anti-rabbit IgG secondary Ab at 1:15000 in 1% milk in TBST for 1 hr at RT. Chemiluminescence performed using SuperSignal West Dura. Data courtesy of Innovators Program.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-FG4R-Immunocytochemistry-Immunofluorescence-NBP2-37823-img0010.jpg)
![Western Blot: A. aeolicus BPL/BioID2 Antibody (SS 3A5-E2) [NBP2-59941] - Analysis in total cell lysate of parental HeLa cell line (lane 1) or HeLa polyclonal cell line stably expressing BioID2-TorsinA delta E302/3 without (lane 2) or with induced expression (lane 3) were transferred to a nitrocellulose membrane and blotted for BPL R40G/BioID2 with SS 3A5-E2 antibody. The predicted molecular weight of BioID2-Torsin A Delta E302/3 fusion protein is 65 kDa](http://images.novusbio.com/fullsize/A-aeolicus-BPL-BioID2-Antibody-SS-3A5-E2-Western-Blot-NBP2-59941-img0007.jpg "Western Blot: A. aeolicus BPL/BioID2 Antibody (SS 3A5-E2) [NBP2-59941] - Analysis in total cell lysate of parental HeLa cell line (lane 1) or HeLa polyclonal cell line stably expressing BioID2-TorsinA delta E302/3 without (lane 2) or with induced expression (lane 3) were transferred to a nitrocellulose membrane and blotted for BPL R40G/BioID2 with SS 3A5-E2 antibody. The predicted molecular weight of BioID2-Torsin A Delta E302/3 fusion protein is 65 kDa")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: A. aeolicus BPL/BioID2 Antibody (SS 3A5-E2) [NBP2-59941] - A) Parental HEK293T or (B) monoclonal HEK293T cell line with BioID2 knocked-in into at least one LMNA gene alleles (resulting in BioID2 Lamin A/C fusion protein) were immunofluorescently labelled with anti-BPL R40G/BioID2 SS 3A5-E2 monoclonal antibody (green). The nuclei were visualized with Hoechst.](http://images.novusbio.com/fullsize/A-aeolicus-BPL-BioID2-Antibody-SS-3A5-E2-Immunocytochemistry-Immunofluorescence-NBP2-59941-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: A. aeolicus BPL/BioID2 Antibody (SS 3A5-E2) [NBP2-59941] - Staining of HeLa polyclonal cell line expressing BioID2 tagged to TorsinA delta E302/3 with SS 3A5-E2 monoclonal antibody. Cells were fixed in (A) cold methanol or (B) 4% PFA and stained with SS anti-BPL R40G/BioID2 3A5-E2 antibody (in green). The nuclei were counter-stained with Hoechst.](http://images.novusbio.com/fullsize/A-aeolicus-BPL-BioID2-Antibody-SS-3A5-E2-Immunocytochemistry-Immunofluorescence-NBP2-59941-img0002.jpg)
![Western Blot: AU5 Epitope Tag Antibody [NB600-461] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.2 ug/ml (1:5,000).](http://images.novusbio.com/fullsize/AU5-Epitope-Tag-Antibody-Western-Blot-NB600-461-img0002.jpg "Western Blot: AU5 Epitope Tag Antibody [NB600-461] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.2 ug/ml (1:5,000).")