A. aeolicus BPL/BioID2 Antibody (SS QD1)

Images

 
Western Blot: A. aeolicus BPL/BioID2 Antibody (SS QD1) [NBP2-59940] - Analysis in total cell lysate of parental HeLa cell line (lane 1) or HeLa polyclonal cell line expressing BioID2 tagged to TorsinA delta E302/3 (6) ...read more
Immunocytochemistry/ Immunofluorescence: A. aeolicus BPL/BioID2 Antibody (SS QD1) [NBP2-59940] - Staining of HeLa cell line expressing doxycycline (dox) - inducible BioID2 tagged to TorsinA delta E302/3 with SS QD1 ...read more
Simple Western: A. aeolicus BPL/BioID2 Antibody (SS QD1) [NBP2-59940] - Lane 1: lysate from untransfected HEK293 cells. Lane 2: lysate from HEK293 cells transfected with BioID2-PTEN. A specific signal for BioID2-PTEN is ...read more

Product Details

Summary
Reactivity BaSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clone
SS QD1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

A. aeolicus BPL/BioID2 Antibody (SS QD1) Summary

Immunogen
GST fused to A. aeolicus BPL (Biotin Protein Ligase) R40G (BioID2)
Specificity
This antibody is reactive with A. aeolicus and has not been tested with other species. The birA Entrez Gene number is NP_213397. UniProt number is O66837.
Isotype
IgG2 Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • Simple Western
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
Application Notes
This antibody is reactive with A. aeolicus and has not been tested on other species. Simple Western was validated through a customer review.
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP2-59940 in the following applications:

Reactivity Notes

Aquifex aeolicus

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Notes

Positive Control: Cells overexpressing A. aeolicus BPL R40G (BioID2) construct.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for A. aeolicus BPL/BioID2 Antibody (NBP2-59940) (0)

There are no publications for A. aeolicus BPL/BioID2 Antibody (NBP2-59940).
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Review for A. aeolicus BPL/BioID2 Antibody (NBP2-59940) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-59940:
Filter by Applications
Simple Western
(1)
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Images Ratings Applications Species Date Details
Simple Western A. aeolicus BPL/BioID2 NBP2-59940
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4
reviewed by:
Kurt Haas
Simple Western Human 11/15/2018
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Summary

ApplicationSimple Western
Sample TestedHEK293 cells transfected with BioID2-PTEN
SpeciesHuman
LotCRT/18/24

Comments

CommentsFor Simple Western (see review image):

Lysates were diluted to 0.3125mg/mL before combining with 5x Fluorescent Master Mix and subjecting samples to Simple Western using default assay parameters (Protein Simple). Primary antibody to BioID2 (NBP2-59940) that was used in assay was diluted to 5ug/mL (1:200) in Antibody Diluent 2 (Protein Simple).

Product review code: SW-1WB

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for A. aeolicus BPL/BioID2 Antibody (NBP2-59940) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-59940AF350
Alexa Fluor 405 NBP2-59940AF405
Alexa Fluor 488 NBP2-59940AF488
Alexa Fluor 532 NBP2-59940AF532
Alexa Fluor 594 NBP2-59940AF594
Alexa Fluor 647 NBP2-59940AF647
Alexa Fluor 700 NBP2-59940AF700
Alexa Fluor 750 NBP2-59940AF750
Biotin NBP2-59940B
DyLight 350 NBP2-59940UV
DyLight 405 NBP2-59940V
DyLight 488 NBP2-59940G
DyLight 550 NBP2-59940R
DyLight 594 NBP2-59940DL594
DyLight 650 NBP2-59940C
DyLight 680 NBP2-59940FR
DyLight 755 NBP2-59940IR
FITC NBP2-59940F
HRP NBP2-59940H
Janelia Fluor 549 NBP2-59940JF549
Janelia Fluor 646 NBP2-59940JF646

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Kurt Haas
11/15/2018
Application: Simple Western
Species: Human