A. aeolicus BPL/BioID2 Antibody (SS QD1) - BSA Free

Western Blot: A. aeolicus BPL/BioID2 Antibody (SS QD1) [NBP2-59940] - Analysis in total cell lysate of parental HeLa cell line (lane 1) or HeLa polyclonal cell line expressing BioID2 tagged to TorsinA delta E302/3 (6) without (lane 2) or with induced expression (lane 3) were transferred to a nitrocellulose membrane and blotted for BPL R40G/BioID2 with SS QD1 antibody. The predicted molecular weight of BioID2-TorsinA delta E302/3 fusion protein is 65 kDa.

Immunocytochemistry/Immunofluorescence: A. aeolicus BPL/BioID2 Antibody (SS QD1) [NBP2-59940] - Staining of HeLa cell line expressing doxycycline (dox) - inducible BioID2 tagged to TorsinA delta E302/3 with SS QD1 monoclonal antibody. Uninduced (-dox) or induced (+dox) cells were fixed in (A) cold methanol or (B) 4% PFA and stained with anti-BPL R40G/BioID2 SS QD1 antibody (in green). The nuclei were counter-stained with Hoechst.

Simple Western: A. aeolicus BPL/BioID2 Antibody (SS QD1) [NBP2-59940] - Lane 1: lysate from untransfected HEK293 cells. Lane 2: lysate from HEK293 cells transfected with BioID2-PTEN. A specific signal for BioID2-PTEN is visible at ~94kDa.

• Reactivity: Ba
• Applications: WB, Simple Western, ICC/IF
• Clone: SS QD1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

A. aeolicus BPL/BioID2 Antibody (SS QD1) - BSA Free Summary

• Immunogen: GST fused to A. aeolicus BPL/BioID2
• Isotype: IgG2 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Simple Western
  • Western Blot 1:100 - 1:2000
• Application Notes: Positive Control: Cells overexpressing A. aeolicus BPL/BioID2 construct.
• Theoretical MW: 27 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Aquifex aeolicus

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for A. aeolicus BPL/BioID2 Antibody (NBP2-59940)(1)

Publication using NBP2-59940

• Malinova D, Wasim L, Newman R Et al. Endophilin A2 regulates B-cell endocytosis and is required for germinal center and humoral responses EMBO reports 2021-07-29 [PMID: 34323351] (ICC/IF)

Review for A. aeolicus BPL/BioID2 Antibody (NBP2-59940) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Kurt Haas Simple Western Human 11/15/2018

Summary

• Application: Simple Western
• Sample Tested: HEK293 cells transfected with BioID2-PTEN
• Species: Human
• Lot: CRT/18/24

Comments

• Comments: For Simple Western (see review image):
  
  Lysates were diluted to 0.3125mg/mL before combining with 5x Fluorescent Master Mix and subjecting samples to Simple Western using default assay parameters (Protein Simple). Primary antibody to BioID2 (NBP2-59940) that was used in assay was diluted to 5ug/mL (1:200) in Antibody Diluent 2 (Protein Simple).
  
  Product review code: SW-1WB