Biotin Antibody [Texas Red]

Western Blot: Biotin Antibody [Texas Red] [NB110-3647] - Lane 1: Biotin conjugated Guinea Pig IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Texas Red goat secondary antibody at 1:1,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Guinea Pig IgG. Other band(s): None.

• Reactivity: All-NA
• Applications: WB, ELISA, ICC/IF, KO
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Texas Red

Biotin Antibody [Texas Red] Summary

• Description: For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room
  
  This product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, biotinylated bovine serum albumin and biotinylated IgG.
• Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (b-KLH)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Fluorophore-linked immunosorbent assay 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:500-1:500
  • Knockout Validated
  • Western Blot 1:100-1:2000
• Application Notes: This product has been tested by dot blot and western blot. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
• Preservative: 0.01% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Biotin Antibody [Texas Red]

• Biotin
• Vitamin B4

Background

Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.