| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50416-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50034SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| HRP | NBP2-16773 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59941-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52637-0.025mg | |||
![Immunohistochemistry-Paraffin: Goat IgG Isotype Control [NB410-28088] - Human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (NB410-28088; left) and Goat Anti-Type III Collagen-UNLB (NBP1-26547; right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP DAB, and hematoxylin.](http://images.novusbio.com/fullsize/Goat-IgG-Isotype-Control-Immunohistochemistry-Paraffin-NB410-28088-img0005.jpg "Immunohistochemistry-Paraffin: Goat IgG Isotype Control [NB410-28088] - Human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (NB410-28088; left) and Goat Anti-Type III Collagen-UNLB (NBP1-26547; right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP DAB, and hematoxylin.")
![Immunohistochemistry-Paraffin: Goat IgG Isotype Control [NB410-28088] - Human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (NB410-28088; left) and Goat Anti-Type IV Collagen-UNLB (NBP1-26549 right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP DAB, and hematoxylin.](http://images.novusbio.com/fullsize/Goat-IgG-Isotype-Control-Immunohistochemistry-Paraffin-NB410-28088-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: GFP Antibody [NBP2-50034] - Analysis of transfected HEK293 cells with a GFP-construct, in green, and stained with chicken pAb to GFP, NBP2-50034, dilution 1:1,000, in red. The blue is DAPI staining of nuclear DNA. NBP2-50034 antibody reveals GFP protein expressed only in transfected cells, and as a result these cells appear golden yellow in color.](http://images.novusbio.com/fullsize/GFP-Antibody-Immunofluorescence-NBP2-50034-img0003.jpg "Immunocytochemistry/Immunofluorescence: GFP Antibody [NBP2-50034] - Analysis of transfected HEK293 cells with a GFP-construct, in green, and stained with chicken pAb to GFP, NBP2-50034, dilution 1:1,000, in red. The blue is DAPI staining of nuclear DNA. NBP2-50034 antibody reveals GFP protein expressed only in transfected cells, and as a result these cells appear golden yellow in color.")
![GFP Antibody [NBP2-50034] - Analysis of HEK293 cell lysates using goat pAb to GFP, dilution 1:1,000 (Green): [1] protein standard, [2] non-transfected control cells, [3] cells transfected with a GFP construct and [4] cells transfected with an mCherry construct. Strong band at ~27kDa corresponds to GFP protein detected only in cells transfected with GFP construct. This antibody does not recognize the mCherry protein. The blot was simultaneously probed with chicken pAb to HSP60, dilution 1:10,000 (Red). The single band at 60kDa represents HSP60 protein expressed in all preparations.](http://images.novusbio.com/fullsize/GFP-Antibody-NBP2-50034-img0004.jpg)
![Western Blot: His Tag Antibody [NBP1-25939] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used for WB at 0.2 ug/ml (1:5,000). Detection: Chemiluminescence withan exposure time of 3 minutes.](http://images.novusbio.com/fullsize/His-Tag-Antibody-Western-Blot-NBP1-25939-img0001.jpg "Western Blot: His Tag Antibody [NBP1-25939] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used for WB at 0.2 ug/ml (1:5,000). Detection: Chemiluminescence withan exposure time of 3 minutes.")
![Western Blot: S-Tag Epitope Tag Antibody [NB600-511] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/ml (1:25,000).](http://images.novusbio.com/fullsize/S-Tag-Epitope-Tag-Antibody-Western-Blot-NB600-511-img0002.jpg "Western Blot: S-Tag Epitope Tag Antibody [NB600-511] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/ml (1:25,000).")
![Western Blot: HSV Tag Antibody [NB600-513] - Analysis using the HRP conjugate of NB600-513. Detection of 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/ml (1:25,000).](http://images.novusbio.com/fullsize/HSV-Tag-Antibody-Western-Blot-NB600-513-img0003.jpg "Western Blot: HSV Tag Antibody [NB600-513] - Analysis using the HRP conjugate of NB600-513. Detection of 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/ml (1:25,000).")
![Western Blot: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588] - Lane 1: Mouse IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Rhodamine goat secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Goat IgG. Other band(s): none.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-F-ab-2-Secondary-Antibody-Western-Blot-NB7588-img0006.jpg "Western Blot: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588] - Lane 1: Mouse IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Rhodamine goat secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Goat IgG. Other band(s): none.")
![Immunocytochemistry/Immunofluorescence: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588]](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-F-ab-2-Secondary-Antibody-Immunocytochemistry-Immunofluorescence-NB7588-img0002.jpg)
![Immunohistochemistry: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588] - Analysis of paraffin embedded human colonic tissue. Image from verified customer review.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-F-ab-2-Secondary-Antibody-Immunohistochemistry-NB7588-img0005.jpg)
![Flow Cytometry: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588]](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-F-ab-2-Secondary-Antibody-Flow-Cytometry-NB7588-img0004.jpg)
![Immunohistochemistry: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588] - Analysis of mouse frozen tissue. Goat anti-Mouse Cy5 was used as a secondary antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-F-ab-2-Secondary-Antibody-Immunohistochemistry-NB7588-img0003.jpg)
![Dot Blot: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588] - Dots are Mouse IgG: (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: none. Secondary Antibody: Goat anti-Mouse IgG Secondary Antibody (Pre-absorbed) Conjugate at 1ug/mL blocked for 1hr at RT. Imaged with BioRad ChemiDoc, Fluorescein filter. Image from the Cy5.5 version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-F-ab-2-Secondary-Antibody-Dot-Blot-NB7588-img0008.jpg)
![Dot Blot: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588] - Mouse IgG. Load: Lane 1 - 50ng Lane 2 - 16.67ng Lane 3 - 5.56ng Lane 4 - 1.85ng Lane 5 - 0.62ng](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-F-ab-2-Secondary-Antibody-Dot-Blot-NB7588-img0007.jpg)
![Western Blot: HA Tag Antibody [NBP2-21581] - Analysis in human HEK293T cells overexpressed with Ly6K-HA. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/HA-Tag-Antibody-Western-Blot-NBP2-21581-img0017.jpg "Western Blot: HA Tag Antibody [NBP2-21581] - Analysis in human HEK293T cells overexpressed with Ly6K-HA. Western blot image submitted by a verified customer review.")
![Flow Cytometry: HA Tag Antibody [NBP2-21581] - HEK293T cells overexpressed with Ly6K-HA stained with HA Epitope Tag Antibody (red) or secondary only (black). Flow cytometry image submitted by a verified customer review.](http://images.novusbio.com/fullsize/HA-Tag-Antibody-Flow-Cytometry-NBP2-21581-img0013.jpg)
![Simple Western: His Tag Antibody (359) [NBP2-43536] - Human purified protein. Simple Western image submitted by a verified customer review.](http://images.novusbio.com/fullsize/His-Tag-Antibody-359-Simple-Western-NBP2-43536-img0009.jpg "Simple Western: His Tag Antibody (359) [NBP2-43536] - Human purified protein. Simple Western image submitted by a verified customer review.")
![Immunocytochemistry/Immunofluorescence: His Tag Antibody (359) [NBP2-43536] - Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: 6X His tag stained by 6X His tag antibody [359] diluted at 1:1000.Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/His-Tag-Antibody-359-Immunocytochemistry-Immunofluorescence-NBP2-43536-img0013.jpg)
![Western Blot: His Tag Antibody (359) [NBP2-43536] - 6x His antibody detects 6x His protein by Western blot analysis. A. Non-induced cell lysates from E. coli expressing HIS-tagged proteins. B. IPTG-induced cell lysates from E. coli expressing HIS-tagged proteins. 15 % SDS-PAGE. 6x His antibody dilution: 1:5000.](http://images.novusbio.com/fullsize/His-Tag-Antibody-359-Western-Blot-NBP2-43536-img0011.jpg)
![Western Blot: His Tag Antibody (359) [NBP2-43536] - Analysis of cell lysate/extract of His-fused gene transfected HeLa cells A. 1/20 lysate/extract of transfected HeLa cells B. Control with 2 ug of preimmune mouse IgG C. Immunoprecipitation of His-fused protein by 2 ug of 6X His tag antibody [359] 12% SDS-PAGE The immunoprecipitated His-fused protein was detected by 6X His tag antibody [359] diluted at 1:1000.](http://images.novusbio.com/fullsize/6-Histidine-Epitope-Tag-Antibody-359-Western-Blot-NBP2-43536-img0003.jpg)
![ELISA: His Tag Antibody (359) [NBP2-43536] - 6X His tag antibody [359] detects His-tagged recombinant proteins by indirect ELISA analysis. Various dilutions of His-tagged recombinant proteins coated onto the 96-well plate were detected by using 0.5 ug/mL 6X His tag antibody [359] . Bound Abs were further detected by using HRP-conjugated goat anti-mouse IgG with HRP substrates.](http://images.novusbio.com/fullsize/6-Histidine-Epitope-Tag-Antibody-359-ELISA-NBP2-43536-img0001.jpg)
![Western Blot: His Tag Antibody (359) [NBP2-43536] - 6x His antibody detects 6x His protein by Western blot analysis.<br />Lane A and B were IPTG-induced cell lysate from E. coli expressing different GST-tagged proteins. <br />Lane C to G were IPTG induced cell lysate from E. coli expressing different His-tagged protein. <br />12 % SDS-PAGE<br />6x His antibody (NBP2-43536) dilution: 1:5000](http://images.novusbio.com/fullsize/nbp2-43536_mouse-monoclonal-his-tag-antibody-359-91020242075922.jpg)
![Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with 6X His tag antibody [GT359] (NBP2-43536) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43536_mouse-monoclonal-his-tag-antibody-359-western-blot-261120251933919.jpg)
![Western Blot: A. aeolicus BPL/BioID2 Antibody (SS 3A5-E2) [NBP2-59941] - Analysis in total cell lysate of parental HeLa cell line (lane 1) or HeLa polyclonal cell line stably expressing BioID2-TorsinA delta E302/3 without (lane 2) or with induced expression (lane 3) were transferred to a nitrocellulose membrane and blotted for BPL R40G/BioID2 with SS 3A5-E2 antibody. The predicted molecular weight of BioID2-Torsin A Delta E302/3 fusion protein is 65 kDa](http://images.novusbio.com/fullsize/A-aeolicus-BPL-BioID2-Antibody-SS-3A5-E2-Western-Blot-NBP2-59941-img0007.jpg "Western Blot: A. aeolicus BPL/BioID2 Antibody (SS 3A5-E2) [NBP2-59941] - Analysis in total cell lysate of parental HeLa cell line (lane 1) or HeLa polyclonal cell line stably expressing BioID2-TorsinA delta E302/3 without (lane 2) or with induced expression (lane 3) were transferred to a nitrocellulose membrane and blotted for BPL R40G/BioID2 with SS 3A5-E2 antibody. The predicted molecular weight of BioID2-Torsin A Delta E302/3 fusion protein is 65 kDa")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: A. aeolicus BPL/BioID2 Antibody (SS 3A5-E2) [NBP2-59941] - A) Parental HEK293T or (B) monoclonal HEK293T cell line with BioID2 knocked-in into at least one LMNA gene alleles (resulting in BioID2 Lamin A/C fusion protein) were immunofluorescently labelled with anti-BPL R40G/BioID2 SS 3A5-E2 monoclonal antibody (green). The nuclei were visualized with Hoechst.](http://images.novusbio.com/fullsize/A-aeolicus-BPL-BioID2-Antibody-SS-3A5-E2-Immunocytochemistry-Immunofluorescence-NBP2-59941-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: A. aeolicus BPL/BioID2 Antibody (SS 3A5-E2) [NBP2-59941] - Staining of HeLa polyclonal cell line expressing BioID2 tagged to TorsinA delta E302/3 with SS 3A5-E2 monoclonal antibody. Cells were fixed in (A) cold methanol or (B) 4% PFA and stained with SS anti-BPL R40G/BioID2 3A5-E2 antibody (in green). The nuclei were counter-stained with Hoechst.](http://images.novusbio.com/fullsize/A-aeolicus-BPL-BioID2-Antibody-SS-3A5-E2-Immunocytochemistry-Immunofluorescence-NBP2-59941-img0002.jpg)
![Western Blot: E-Tag Epitope Tag Antibody [NB600-518] - Detection of E-Tag-tagged Protein by Western Blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, goat anti-E-Tag antibody NB600-518 used for WB at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/E-Tag-Epitope-Tag-Antibody-Western-Blot-NB600-518-img0004.jpg "Western Blot: E-Tag Epitope Tag Antibody [NB600-518] - Detection of E-Tag-tagged Protein by Western Blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, goat anti-E-Tag antibody NB600-518 used for WB at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Simple Western: His Tag Antibody (3D5) - Chimeric [NBP2-52637] - His Tag Antibody (3D5) [NBP2-52637] - Human purified protein. Simple Western image submitted by a verified customer review.](http://images.novusbio.com/fullsize/His-Tag-Antibody-3D5-Simple-Western-NBP2-52637-img0002.jpg "Simple Western: His Tag Antibody (3D5) - Chimeric [NBP2-52637] - His Tag Antibody (3D5) [NBP2-52637] - Human purified protein. Simple Western image submitted by a verified customer review.")
![Western Blot: His Tag Antibody (3D5) - Chimeric - Azide and BSA Free [NBP2-52637] - His tag expressing plasmid transfected (A) and non-transfected (B) HEK293 cells lysate (3g protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with NBP2-52637 at 0.01ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp2-52637_rabbit-monoclonal-his-tag-antibody-3d5-chimeric-74202522141228.png)
![Immunocytochemistry /Immunofluorescence: His Tag Antibody (3D5) - Chimeric - Azide and BSA Free [NBP2-52637] - Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells transfected with His tag expressing plasmid (A) and non-transfected HEK293 cells (B), permeabilized with 0.15% Triton stained with NBP2-52637 (1:200 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue).](http://images.novusbio.com/fullsize/nbp2-52637_rabbit-monoclonal-his-tag-antibody-3d5-chimeric-74202522141211.png)