His Tag Antibody (AD1.1.10) [Biotin]

Images

 
Western Blot: 6-Histidine Epitope Tag Antibody (AD1.1.10) [Biotin] [NB100-63172] - Western blot of HIS tagged protein probed with mouse anti histidine tag : Biotin and visualised with Streptavidin : HRP.

Product Details

Summary
Reactivity Hu, Dr, NASpecies Glossary
Applications WB, ELISA
Clone
AD1.1.10
Clonality
Monoclonal
Host
Mouse
Conjugate
Biotin

His Tag Antibody (AD1.1.10) [Biotin] Summary

Immunogen
PAX6 transcription factor linked to histidine tag
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • ELISA 1:500-1:1000
Application Notes
This 6-Histidine Epitope Tag Antibody (AD1.1.10) is useful for Western blot, Immunohistochemistry on frozen sections, ELISA, Flow Cytometry and Immunoprecipitation. This antibody is routinely tested in Western blot using Histidine-tagged recombinant proteins.
Readout System

Reactivity Notes

This antibody is reactive to histidine tags.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for His Tag Antibody (AD1.1.10) [Biotin]

  • 6 His epitope tag
  • 6X His
  • 6X-His
  • H
  • HHHHHH epitope tag
  • HHHHHH tag
  • His Tag
  • HIS
  • polyHistidine
  • Poly-histidine

Background

The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

HRP Labeled NB100-63173

Additional His Tag Products

His Tag NB100-63172

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